CN103203257A - Method for manufacturing paper micro-fluidic chip - Google Patents
Method for manufacturing paper micro-fluidic chip Download PDFInfo
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- CN103203257A CN103203257A CN2013100777966A CN201310077796A CN103203257A CN 103203257 A CN103203257 A CN 103203257A CN 2013100777966 A CN2013100777966 A CN 2013100777966A CN 201310077796 A CN201310077796 A CN 201310077796A CN 103203257 A CN103203257 A CN 103203257A
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Abstract
The invention provides a method for manufacturing a paper micro-fluidic chip. The method includes injecting a material used for forming micro-channels into a penholder of a sample injection pen and sealing the material in the penholder by sealing oil after the material is injected into the penholder; distributing the material on paper by means of writing by the aid of the sample injection pen according to shapes of the micro-channels; and baking the paper after the material seeps into the paper so that the material seeping into the paper is solidified to obtain the micro-fluidic chip. The sample injection pen further comprises a nib, a sample outflow hole is arranged at the front end of the nib, a rotatable ball is arranged in the sample outflow hole, and the rear end of the nib is communicated with the penholder. The trace material used for forming the micro-channels is distributed on a substrate of the chip by means of writing by the aid of the sample injection pen, the micro-fluidic chip is obtained by means of solidifying the material after the material seeps into the substrate, and the method has the advantages of low cost, simplicity in operation, flexibility in use, controllability and the like.
Description
Technical field
The invention belongs to biological technology application, be specifically related to a kind of method for the preparation of the papery micro-fluid chip.
Background technology
But the micro-fluid chip based on paper has characteristics such as the low disposable of cost, and because the capillarity in the paper does not need the external world that the power of liquid flow is provided, therefore enjoys favor.Yet the method in paper processing microchannel is single at present, generally need utilize the wax spray printer.This method cost height needs computer to assist, and underaction can not satisfy market for the needs of low-cost, flexible processing method.
Summary of the invention
The object of the present invention is to provide a kind of method for preparing the papery micro-fluid chip simply, conveniently, flexibly, cheaply.
For achieving the above object, the present invention has adopted following technical scheme:
1) material that will be used to form the microchannel injects the penholder of application of sample pen, described application of sample pen also comprises nib, the front end of nib offers sample flow and portals, in portalling, sample flow is provided with rotary ball, the rear end of nib is connected with penholder, after injecting described material, with Seal Oil with material seal in penholder;
2) described material is write on paper with the application of sample pen according to the shape of microchannel;
3) after material infiltrates in the paper, paper is toasted, make the material cured of infiltrating in the paper, get micro-fluid chip.
Described application of sample pen also comprises for connecting the jockey of nib with penholder.
The described surface that refers to end in contact paper before the nib of writing, it is mobile at paper to drive nib then, and the ball of written front end is rotated, and the material that drives in the penholder flows out, and the material of outflow is coated on the paper.
The diameter of described ball is 100nm-1cm.
The application of sample weight range of described application of sample pen is 10-1000nL/cm.
The described material that is used to form the microchannel is the mixture of dimethyl silicone polymer and crosslinking agent.
The present invention utilizes the application of sample pen to write the micro-material that is used to form the microchannel in chip base, after material infiltrates through substrate, obtains micro-fluid chip by curing, has characteristics such as with low cost, that operation is simple and easy, use is flexible, controlled.
Description of drawings
Fig. 1 is the structural representation of application of sample pen of the present invention; Among Fig. 1: 1 nib, 2 penholders, 3 balls, 4 jockeys, 5 are used to form the material of microchannel, 6 Seal Oil;
Fig. 2 is the papery micro-fluid chip pictorial diagram (plane) of the present invention's preparation;
Fig. 3 is the papery micro-fluid chip pictorial diagram (curved surface) of the present invention's preparation;
Fig. 4 is the use state diagram of papery micro-fluid chip shown in Figure 2; Wherein, a is that red pigment adds the initial position on the micro-fluid chip, and b is the distributing position of red pigment on micro-fluid chip;
Fig. 5 is that an application of sample of the present invention application of sample amount is with the variation of writing speed;
Fig. 6 is that an application of sample of the present invention application of sample amount is with the variation of front end ball size;
Fig. 7 is the variation of an application of sample of the present invention application of sample amount with penholder inner stuffing (being used to form the material of microchannel) height.
The specific embodiment
The invention will be further described below in conjunction with drawings and Examples.
The method for preparing the papery micro-fluid chip of the present invention, may further comprise the steps: the material that 1) will be used to form the microchannel injects the penholder 2 of application of sample pen, the described material that is used to form the microchannel is the mixture of dimethyl silicone polymer and crosslinking agent, described application of sample pen also comprises nib 1, the front end of nib 1 offers sample flow and portals, in portalling, sample flow is provided with rotary ball 3, the rear end of nib 1 is connected with penholder 2, after injecting described material, with Seal Oil 6 with material seal in penholder 2, described application of sample pen also comprises for connecting the jockey 4 of nib 1 with penholder 2, referring to Fig. 1; 2) described material is write on paper with the application of sample pen according to the shape of microchannel, the described surface that refers to end in contact paper before the nib 1 of writing, it is mobile at paper to drive nib 1 then, the ball 3 of written 1 front end is rotated, the material that drives in the penholder 2 flows out, the material that flows out is coated on the paper, can realize micro sample-adding; 3) after material infiltrates in the paper, paper is toasted, make the material cured of infiltrating in the paper, get micro-fluid chip.
The diameter of described ball 2 is 100nm-1cm.The application of sample weight range of described application of sample pen is 10-1000nL/cm.By adjusting the application of sample amount of writing speed (Fig. 5), ball diameter (Fig. 6), penholder inner stuffing height (Fig. 7) change application of sample pen, writing speed refers to that written front end is in the mobile speed of paper, writing speed can be controlled by driving mechanism, simultaneously, can connect the filler supplementary mechanism in the penholder rear end, make the penholder inner stuffing keep stable height.
Embodiment
The structure of biological application of sample pen as shown in Figure 1, formed by 6 parts, nib 1 mainly provides the support point of ball 3, penholder 2 is used for stored sample (mixture of dimethyl silicone polymer and crosslinking agent), ball 3 provides enough shearing forces during frictional rotation in substrate, the drive sample flows out, and jockey 4 is used for connecting penholder 2 and nib 1, and Seal Oil 6 prevents the sample volatilization.When biological application of sample pen (is similar to the writing process of ball pen or gel ink pen) when substrate is write, the ball 3 of written 1 front end rotates, and drives the mixture outflow of dimethyl silicone polymer and crosslinking agent.
Utilize biological application of sample pen to prepare the papery micro-fluid chip, may further comprise the steps:
Step 1: the mixture (10 μ L-2mL) of an amount of dimethyl silicone polymer and crosslinking agent is injected penholder, with the mineral oil sealing, avoid evaporating thereafter.
Step 2: hold this biological application of sample pen and write at paper, make the preceding end in contact paper of nib, it is mobile at paper to drive nib then, and the ball of written front end is rotated, and the mixture of drive dimethyl silicone polymer and crosslinking agent flows out, and the mixture of outflow is coated on the paper.Write line route microchannel is designed and is decided.
Step 3: after mixture infiltrates in the paper, paper is put into baking oven toast, 80 ℃ were toasted 50 minutes, and purpose is that the dimethyl silicone polymer moulding is fixed.
Example 1 utilizes biological application of sample pen to prepare straight channel at paper
Step 1: the mixture of 2mL dimethyl silicone polymer and crosslinking agent (SYLGARD184) is injected penholder, with the mineral oil sealing, avoid evaporating thereafter.
Step 2: according to the microchannel shape of design, hold biological application of sample pen and write at paper, writing circuit is straight line.
Step 3: after mixture infiltrates in the paper, paper is put into baking oven toast, 80 ℃ were toasted 50 minutes, and got the papery micro-fluid chip, referring to Fig. 2.
Step 4: add 20 microlitre orchils on the papery micro-fluid chip, referring to Fig. 4 a, after 10 minutes, orchil flows according to the direction of design, and is dispersed in the microchannel, referring to Fig. 4 b, shows that micro-fluid chip can normally use.
Example 2 utilizes biological application of sample pen to prepare bending channel at paper
Step 1: the mixture of 10 μ L dimethyl silicone polymers and crosslinking agent (SYLGARD184) is injected penholder, with the mineral oil sealing, avoid evaporating thereafter.
Step 2: according to the microchannel shape of design, hold biological application of sample pen and write at paper, writing circuit is curve.
Step 3: after mixture infiltrates in the paper, paper is put into baking oven toast, 80 ℃ were toasted 50 minutes, and got the papery micro-fluid chip, referring to Fig. 3.
Claims (6)
1. a method for preparing the papery micro-fluid chip is characterized in that, may further comprise the steps:
1) material that will be used to form the microchannel injects the penholder (2) of application of sample pen, described application of sample pen also comprises nib (1), the front end of nib (1) offers sample flow and portals, in portalling, sample flow is provided with rotary ball (3), the rear end of nib (1) is connected with penholder (2), after injecting described material, with Seal Oil (6) with material seal in penholder (2);
2) described material is write on paper with the application of sample pen according to the shape of microchannel;
3) after material infiltrates in the paper, paper is toasted, make the material cured of infiltrating in the paper, get micro-fluid chip.
2. according to the described a kind of method for preparing the papery micro-fluid chip of claim 1, it is characterized in that: described application of sample pen also comprises for connecting the jockey (4) of nib (1) with penholder (2).
3. according to the described a kind of method for preparing the papery micro-fluid chip of claim 1, it is characterized in that: the described surface that refers to nib (1) preceding end in contact paper of writing, it is mobile at paper to drive nib (1) then, the ball (3) of nib (1) front end is rotated, the material that drives in the penholder (1) flows out, and the material of outflow is coated on the paper.
4. according to the described a kind of method for preparing the papery micro-fluid chip of claim 1, it is characterized in that: the diameter of described ball (3) is 100nm-1cm.
5. according to the described a kind of method for preparing the papery micro-fluid chip of claim 1, it is characterized in that: the application of sample weight range of described application of sample pen is 10-1000nL/cm.
6. according to the described a kind of method for preparing the papery micro-fluid chip of claim 1, it is characterized in that: the described material that is used to form the microchannel is the mixture of dimethyl silicone polymer and crosslinking agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310077796.6A CN103203257B (en) | 2013-03-12 | 2013-03-12 | Method for manufacturing paper micro-fluidic chip |
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| CN201310077796.6A CN103203257B (en) | 2013-03-12 | 2013-03-12 | Method for manufacturing paper micro-fluidic chip |
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| CN103203257A true CN103203257A (en) | 2013-07-17 |
| CN103203257B CN103203257B (en) | 2015-03-04 |
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| CN201310077796.6A Expired - Fee Related CN103203257B (en) | 2013-03-12 | 2013-03-12 | Method for manufacturing paper micro-fluidic chip |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103394384A (en) * | 2013-08-12 | 2013-11-20 | 广东顺德西安交通大学研究院 | Paper-based microfluidic chip and preparation method thereof |
| CN104209153A (en) * | 2014-09-10 | 2014-12-17 | 南京邮电大学 | Preparation method of paper micro-fluidic chips |
| CN104677896A (en) * | 2015-01-31 | 2015-06-03 | 太原理工大学 | Preparation and application of paper-based microfluidic chip for colorimetric analysis |
| CN105903501A (en) * | 2016-04-18 | 2016-08-31 | 南京大学 | Polydimethylsiloxane-paper composite chip and preparation method and application thereof |
| CN106268990A (en) * | 2016-07-26 | 2017-01-04 | 林捷琳 | A kind of paper micro-flow control chip preparation method based on dot matrix pressure sintering |
| CN106443026A (en) * | 2015-08-10 | 2017-02-22 | 华东理工大学 | Rapid production method of paper-based microfluidic chip |
| CN106485250A (en) * | 2016-09-09 | 2017-03-08 | 华东理工大学 | A kind of recognition methods of paper chip gray value and its application |
| CN106475157A (en) * | 2016-09-29 | 2017-03-08 | 西安交通大学 | A kind of paper substrate micro-fluid chip wax crayon device and its application |
| CN109482247A (en) * | 2018-10-18 | 2019-03-19 | 哈尔滨工业大学（深圳） | A kind of micro-fluidic chip manufacturing process and micro-fluidic chip |
| CN113198554A (en) * | 2021-05-14 | 2021-08-03 | 广州大学 | Method for manufacturing micro-fluidic chip formed rapidly on chip |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5911533A (en) * | 1997-11-24 | 1999-06-15 | Eastman Kodak Company | Microfluidic writing pen |
| CN101099683A (en) * | 2003-08-21 | 2008-01-09 | 环球科技（新西兰）有限公司 | Sampling device |
| CN101585507A (en) * | 2009-06-23 | 2009-11-25 | 中国科学院上海微系统与信息技术研究所 | Method for producing through-hole structure in PDMS micro-fluidic chip |
| CN102614948A (en) * | 2012-04-05 | 2012-08-01 | 北京金智捷生物科技有限公司 | Micro-fluidic chip and manufacturing method thereof |
| WO2012173506A2 (en) * | 2011-06-16 | 2012-12-20 | Institutul National De Cercetare-Dezvoltare Pentru Microtehnologie | Procedure of 2d and 3d optically assisted fountain pen nanolithography and aperture pen nanolithography |
-
2013
- 2013-03-12 CN CN201310077796.6A patent/CN103203257B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5911533A (en) * | 1997-11-24 | 1999-06-15 | Eastman Kodak Company | Microfluidic writing pen |
| CN101099683A (en) * | 2003-08-21 | 2008-01-09 | 环球科技（新西兰）有限公司 | Sampling device |
| CN101585507A (en) * | 2009-06-23 | 2009-11-25 | 中国科学院上海微系统与信息技术研究所 | Method for producing through-hole structure in PDMS micro-fluidic chip |
| WO2012173506A2 (en) * | 2011-06-16 | 2012-12-20 | Institutul National De Cercetare-Dezvoltare Pentru Microtehnologie | Procedure of 2d and 3d optically assisted fountain pen nanolithography and aperture pen nanolithography |
| CN102614948A (en) * | 2012-04-05 | 2012-08-01 | 北京金智捷生物科技有限公司 | Micro-fluidic chip and manufacturing method thereof |
Non-Patent Citations (1)
| Title |
|---|
| ANDRES W.MARTINEZ等: "Programmable diagnostic devices made from paper and tape", 《THE ROYAL SOCIETY OF CHEMISTRY》, no. 10, 30 July 2010 (2010-07-30), pages 2499 - 2504 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103394384A (en) * | 2013-08-12 | 2013-11-20 | 广东顺德西安交通大学研究院 | Paper-based microfluidic chip and preparation method thereof |
| CN103394384B (en) * | 2013-08-12 | 2015-07-22 | 广东顺德西安交通大学研究院 | Paper-based microfluidic chip and preparation method thereof |
| CN104209153A (en) * | 2014-09-10 | 2014-12-17 | 南京邮电大学 | Preparation method of paper micro-fluidic chips |
| CN104677896A (en) * | 2015-01-31 | 2015-06-03 | 太原理工大学 | Preparation and application of paper-based microfluidic chip for colorimetric analysis |
| CN106443026A (en) * | 2015-08-10 | 2017-02-22 | 华东理工大学 | Rapid production method of paper-based microfluidic chip |
| CN105903501A (en) * | 2016-04-18 | 2016-08-31 | 南京大学 | Polydimethylsiloxane-paper composite chip and preparation method and application thereof |
| CN106268990A (en) * | 2016-07-26 | 2017-01-04 | 林捷琳 | A kind of paper micro-flow control chip preparation method based on dot matrix pressure sintering |
| CN106268990B (en) * | 2016-07-26 | 2018-07-24 | 林捷琳 | A kind of paper micro-flow control chip preparation method based on dot matrix pressure sintering |
| CN106485250A (en) * | 2016-09-09 | 2017-03-08 | 华东理工大学 | A kind of recognition methods of paper chip gray value and its application |
| CN106475157A (en) * | 2016-09-29 | 2017-03-08 | 西安交通大学 | A kind of paper substrate micro-fluid chip wax crayon device and its application |
| CN106475157B (en) * | 2016-09-29 | 2019-03-01 | 西安交通大学 | A kind of paper base micro-fluid chip wax crayon device and its application |
| CN109482247A (en) * | 2018-10-18 | 2019-03-19 | 哈尔滨工业大学（深圳） | A kind of micro-fluidic chip manufacturing process and micro-fluidic chip |
| CN109482247B (en) * | 2018-10-18 | 2021-04-13 | 哈尔滨工业大学（深圳） | Micro-fluidic chip manufacturing process and micro-fluidic chip |
| CN113198554A (en) * | 2021-05-14 | 2021-08-03 | 广州大学 | Method for manufacturing micro-fluidic chip formed rapidly on chip |
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| CN103203257B (en) | 2015-03-04 |
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