CN103191679B - A kind of preparation method of microscale hydrogel - Google Patents
A kind of preparation method of microscale hydrogel Download PDFInfo
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- CN103191679B CN103191679B CN201310078473.9A CN201310078473A CN103191679B CN 103191679 B CN103191679 B CN 103191679B CN 201310078473 A CN201310078473 A CN 201310078473A CN 103191679 B CN103191679 B CN 103191679B
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- hydrogel precursor
- hydrogel
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Abstract
The invention provides a kind of preparation method of microscale hydrogel: the penholder 1) hydrogel precursor being injected application of sample pen, described application of sample pen also comprises nib, the front end of nib offers sample tap hole, rotary ball is provided with in sample tap hole, the rear end of nib is connected with penholder, after injecting hydrogel precursor, by Seal Oil, hydrogel precursor is sealed in penholder; 2) with application of sample pen, hydrogel precursor is write in substrate; 3) process is cured to suprabasil hydrogel precursor, the present invention utilizes application of sample pen in substrate, write minor amount of water gel precursors, then make hydrogel precursor crosslinked fixing, form microscale hydrogel, the method has that stock utilization is high, with low cost, easy to use and flexible, processing controllability high.
Description
Technical field
The invention belongs to biological technology application, be specifically related to a kind of preparation method operating the microscale hydrogel of simple and easy, low cost.
Background technology
Hydrogel is the macromolecule network structure of moisture content superelevation (being generally more than 90%), has the feature such as good biocompatibility, intelligence, is widely used in the field such as biology, medical science.Microgel refers to the hydrogel material (being generally micron, Nano grade) within the scope of minute yardstick.Microgel, because size is little and be porous network structure, can be used for medicine carrying, as the pharmaceutical carrier of slowly-releasing, target in medical domain.Meanwhile, due to its good biocompatibility, also can load cell, as " biological brick " in organizational project, build tissue.
Prepare means mainly photomask method, micro-fluidic method and the cell printing method of microgel at present.Wherein, photomask method is method that is the most ripe and extensive use, can be used for the microgel making various shape and yardstick, but this method is only applicable to the gel rubber material of photo-crosslinking, seriously limits the application of this method.Micro-fluidic method and cell printing method can be used for the gel preparation of temperature crosslink.But these three kinds of methods all relate to complicated and tediously long operating process and expensive equipment, and cost of manufacture is high.Therefore, simple to operate, method flexibly, have wide range of applications the extensive use of low cost minute yardstick gel process for preparing to microgel significant.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of microscale hydrogel of easy and simple to handle, flexible, low cost.
For achieving the above object, present invention employs following technical scheme:
1) hydrogel precursor is injected the penholder of application of sample pen, described application of sample pen also comprises nib, the front end of nib offers sample tap hole, rotary ball is provided with in sample tap hole, the rear end of nib is connected with penholder, after injecting hydrogel precursor, by Seal Oil, hydrogel precursor is sealed in penholder;
2) with application of sample pen, hydrogel precursor is write in substrate;
3) process is cured to suprabasil hydrogel precursor.
Described application of sample pen also comprises the jockey for connecting nib and penholder.
Described writing refers to end in contact substrate before nib, then drives nib to move in substrate, the ball of written front end is rotated, and drive the hydrogel precursor in penholder to flow out, the hydrogel precursor of outflow is coated in substrate.
The diameter of described ball is 100nm-1cm.
The application of sample weight range of described application of sample pen is 10-1000nL/cm.
Described solidification process comprises ultraviolet lighting, intensification or cooling.
Beneficial effect of the present invention is:
The present invention utilizes application of sample pen in substrate, write minor amount of water gel precursors, then makes hydrogel precursor crosslinked fixing, forms microscale hydrogel, and the method has that stock utilization is high, with low cost, easy to use and flexible, processing controllability high.
Accompanying drawing explanation
Fig. 1 is the structural representation of application of sample pen of the present invention; In Fig. 1: 1 nib, 2 penholders, 3 balls, 4 jockeys, 5 hydrogel precursors, 6 Seal Oil;
Fig. 2 is wire polyethylene glycol hydrogel effect (in figure, scale is 500 μm) prepared by the present invention;
Fig. 3 is the effect (in figure, scale is 500 μm) of point-like temperature crosslink gelatin microscale hydrogel prepared by the present invention;
Fig. 4 is the change of an application of sample of the present invention application of sample amount with writing speed;
Fig. 5 is the change of an application of sample of the present invention application of sample amount with front end ball size;
Fig. 6 is the change of an application of sample of the present invention application of sample amount with penholder inner stuffing (hydrogel precursor) height.
Detailed description of the invention
Below in conjunction with drawings and Examples, the invention will be further described.
The preparation method of microscale hydrogel of the present invention, comprise the following steps: the penholder 2 1) hydrogel precursor being injected application of sample pen, described application of sample pen also comprises nib 1, the front end of nib 1 offers sample tap hole, rotary ball 3 is provided with in sample tap hole, the rear end of nib 1 is connected with penholder 2, after injecting hydrogel precursor, with Seal Oil 6(mineral oil) hydrogel precursor is sealed in penholder 2, described application of sample pen also comprises the jockey 4 for connecting nib 1 and penholder 2, see Fig. 1; 2) with application of sample pen, hydrogel precursor is write in substrate (as glass plate); Described writing refers to end in contact substrate before nib 1, then drives nib 1 to move in substrate, the ball 3 of written 1 front end is rotated, and drive the hydrogel precursor in penholder 2 to flow out, the hydrogel precursor of outflow is coated in substrate, can realize micro sample-adding; 3) being cured process to suprabasil hydrogel precursor makes suprabasil hydrogel precursor crosslinked fixing; Described solidification process comprises ultraviolet lighting, intensification or cooling.
The diameter of described ball 3 is 100nm-1cm.The application of sample weight range of described application of sample pen is 10-1000nL/cm.The application of sample amount of application of sample pen is changed by hydrogel precursor height (Fig. 6) in adjustment writing speed (Fig. 4), ball diameter (Fig. 5), base material or penholder, writing speed refers to the speed of written front end movement in substrate, writing speed can be controlled by driving mechanism, simultaneously, hydrogel precursor supplementary mechanism can be connected in penholder rear end, make the height that hydrogel precursor in penholder keeps stable.
Embodiment
The structure of biological application of sample pen as shown in Figure 1, be made up of 6 parts, nib 1 mainly provides the support point of ball 3, penholder 2 is for storing hydrogel precursor, ball 3 provides enough shearing forces in substrate during frictional rotation, drive hydrogel precursor 5 to flow out, jockey 4 is for connecting penholder 2 and nib 1, and Seal Oil 6 prevents sample (hydrogel precursor) from volatilizing.When biological application of sample pen is write in substrate (being similar to the writing process of ball pen or gel ink pen), the ball 3 of written 1 front end rotates, and drives hydrogel precursor 5 to flow out.
Utilize biological application of sample pen to prepare hydrogel, comprise the following steps:
Step 1: appropriate required process water gel precursors (10 μ L-2mL) is injected penholder, uses thereafter mineral oil (Seal Oil) to seal, avoid evaporating.
Step 2: hold this biological application of sample pen and write on a glass, make the front end in contact substrate of nib, then nib is driven to move in substrate, the ball of written front end is rotated, drive hydrogel precursor flows out, the hydrogel precursor flowed out is coated in substrate, and hydrogel precursor can write into the difformity such as point-like, wire flexibly.
Step 3: utilize chemical reaction to make suprabasil hydrogel precursor crosslinked fixing (different according to hydrogel kind, as ultraviolet lighting, intensification or cooling etc.), then can form the microscale hydrogel of various shape.
Example 1 utilizes biological application of sample pen to prepare photo-crosslinking polyethylene glycol microscale hydrogel
Step 1: 200 μ L polyethylene glycol hydrogel precursors are injected penholder, thereafter with mineral oil sealing, avoids evaporating.Polyethylene glycol hydrogel precursor composed as follows: the molecular weight of 10% is the polyethylene glycol of 1000, and the 2-hydroxy-2-methyl-1-phenyl-1-acetone of 0.5%, surplus is water.
Step 2: hold this biological application of sample pen and write point-like on a glass.
Step 3: utilize ultraviolet source irradiation 30 seconds, makes suprabasil hydrogel precursor crosslinked fixing, then forms the minute yardstick polyethylene glycol hydrogel (Fig. 2) of point-like.
Example 2 utilizes a biological application of sample preparation temperature cross-linked gelatin microscale hydrogel
Step 1: 200 μ L gelatin precursors are injected penholder, thereafter with mineral oil sealing, avoid evaporating, gelatin precursor is the pigskin gelatin aqueous solution of 3%.
Step 2: hold this biological application of sample pen and write wire on a glass.
Step 3: be cooled to 4 degrees Celsius and suprabasil hydrogel precursor is cross-linked, then can form the microscale hydrogel (Fig. 3) of wire.
Claims (4)
1. a preparation method for microscale hydrogel, is characterized in that, comprises the following steps:
1) hydrogel precursor is injected the penholder (2) of application of sample pen, described application of sample pen also comprises nib (1), the front end of nib (1) offers sample tap hole, rotary ball (3) is provided with in sample tap hole, the rear end of nib (1) is connected with penholder (2), after injecting hydrogel precursor, by Seal Oil (6), hydrogel precursor is sealed in penholder (2);
2) with application of sample pen, hydrogel precursor is write in substrate;
3) process is cured to suprabasil hydrogel precursor;
It is 2cm/s or 4cm/s that writing speed carries out control by driving mechanism, meanwhile, connects hydrogel precursor supplementary mechanism, make the height that hydrogel precursor in penholder keeps stable in penholder rear end;
The diameter of described ball (3) is 100nm-1cm; The application of sample weight range of described application of sample pen is 10-1000nL/cm.
2. the preparation method of a kind of microscale hydrogel according to claim 1, is characterized in that: described application of sample pen also comprises the jockey (4) for connecting nib (1) and penholder (2).
3. the preparation method of a kind of microscale hydrogel according to claim 1, it is characterized in that: described in write and refer to front for nib (1) end in contact substrate, then nib (1) is driven to move in substrate, the ball (3) of nib (1) front end is rotated, drive the hydrogel precursor in penholder (1) to flow out, the hydrogel precursor of outflow is coated in substrate.
4. the preparation method of a kind of microscale hydrogel according to claim 1, is characterized in that: described solidification process comprises ultraviolet lighting, intensification or cooling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310078473.9A CN103191679B (en) | 2013-03-12 | 2013-03-12 | A kind of preparation method of microscale hydrogel |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310078473.9A CN103191679B (en) | 2013-03-12 | 2013-03-12 | A kind of preparation method of microscale hydrogel |
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| Publication Number | Publication Date |
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| CN103191679A CN103191679A (en) | 2013-07-10 |
| CN103191679B true CN103191679B (en) | 2015-10-28 |
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| CN201310078473.9A Expired - Fee Related CN103191679B (en) | 2013-03-12 | 2013-03-12 | A kind of preparation method of microscale hydrogel |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1874902A (en) * | 2003-11-07 | 2006-12-06 | 樱花彩色产品株式会社 | Coating tool |
| CN201782232U (en) * | 2010-07-27 | 2011-04-06 | 雅蒂斯企业有限公司 | Daubing pen structure |
| CN102209640A (en) * | 2008-11-11 | 2011-10-05 | 株式会社樱花彩色笔 | Applicator tip and applicator |
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2013
- 2013-03-12 CN CN201310078473.9A patent/CN103191679B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1874902A (en) * | 2003-11-07 | 2006-12-06 | 樱花彩色产品株式会社 | Coating tool |
| CN102209640A (en) * | 2008-11-11 | 2011-10-05 | 株式会社樱花彩色笔 | Applicator tip and applicator |
| CN201782232U (en) * | 2010-07-27 | 2011-04-06 | 雅蒂斯企业有限公司 | Daubing pen structure |
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| CN103191679A (en) | 2013-07-10 |
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