CN103203257B - Method for manufacturing paper micro-fluidic chip - Google Patents
Method for manufacturing paper micro-fluidic chip Download PDFInfo
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- CN103203257B CN103203257B CN201310077796.6A CN201310077796A CN103203257B CN 103203257 B CN103203257 B CN 103203257B CN 201310077796 A CN201310077796 A CN 201310077796A CN 103203257 B CN103203257 B CN 103203257B
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Abstract
The invention provides a method for manufacturing a paper micro-fluidic chip. The method includes injecting a material used for forming micro-channels into a penholder of a sample injection pen and sealing the material in the penholder by sealing oil after the material is injected into the penholder; distributing the material on paper by means of writing by the aid of the sample injection pen according to shapes of the micro-channels; and baking the paper after the material seeps into the paper so that the material seeping into the paper is solidified to obtain the micro-fluidic chip. The sample injection pen further comprises a nib, a sample outflow hole is arranged at the front end of the nib, a rotatable ball is arranged in the sample outflow hole, and the rear end of the nib is communicated with the penholder. The trace material used for forming the micro-channels is distributed on a substrate of the chip by means of writing by the aid of the sample injection pen, the micro-fluidic chip is obtained by means of solidifying the material after the material seeps into the substrate, and the method has the advantages of low cost, simplicity in operation, flexibility in use, controllability and the like.
Description
Technical field
The invention belongs to biological technology application, be specifically related to a kind of method for the preparation of papery micro-fluid chip.
Background technology
Micro-fluid chip based on paper have cost low, the feature such as arbitrarily can to dispose, and do not need the external world to provide the power of liquid flow due to the capillarity in paper, therefore enjoy favor.But the method for processing microchannel at present on paper is single, generally needs to utilize wax spray printer.The method cost is high, needs computer to assist, underaction, can not meet the needs of market for low cost, flexible processing method.
Summary of the invention
The object of the present invention is to provide a kind of simple, convenient, flexibly, the method preparing papery micro-fluid chip of low cost.
For achieving the above object, present invention employs following technical scheme:
1) material for the formation of microchannel is injected the penholder of application of sample pen, described application of sample pen also comprises nib, the front end of nib offers sample tap hole, rotary ball is provided with in sample tap hole, the rear end of nib is connected with penholder, after injecting described material, with Seal Oil by material seal in penholder;
2) according to the shape application of sample pen of microchannel, described material is write on paper;
3) when material infiltrates after in paper, paper is toasted, makes the material cured in infiltration paper, obtain micro-fluid chip.
Described application of sample pen also comprises the jockey for connecting nib and penholder.
Described writing refers to the surface of end in contact paper before nib, then drives nib to move on paper, the ball of written front end is rotated, and drive the material outflow in penholder, the material of outflow is coated on paper.
The diameter of described ball is 100nm-1cm.
The application of sample weight range of described application of sample pen is 10-1000nL/cm.
The described material for the formation of microchannel is the mixture of dimethyl silicone polymer and crosslinking agent.
The present invention utilize application of sample pen write in chip base trace the material for the formation of microchannel, after infiltration enters substrate, by solidification obtain micro-fluid chip, have with low cost, operate simple and easy, use the features such as flexible, controlled.
Accompanying drawing explanation
Fig. 1 is the structural representation of application of sample pen of the present invention; In Fig. 1: 1 nib, 2 penholders, 3 balls, 4 jockeys, 5 for the formation of the material of microchannel, 6 Seal Oil;
Fig. 2 is papery micro-fluid chip pictorial diagram (plane) prepared by the present invention;
Fig. 3 is papery micro-fluid chip pictorial diagram (curved surface) prepared by the present invention;
Fig. 4 is the using state figure of the micro-fluid chip of papery shown in Fig. 2; Wherein, a is the initial position that red pigment adds on micro-fluid chip, and b is the distributing position of red pigment on micro-fluid chip;
Fig. 5 is the change of an application of sample of the present invention application of sample amount with writing speed;
Fig. 6 is the change of an application of sample of the present invention application of sample amount with front end ball size;
Fig. 7 is the change of an application of sample of the present invention application of sample amount with penholder inner stuffing (material for the formation of microchannel) height.
Detailed description of the invention
Below in conjunction with drawings and Examples, the invention will be further described.
The method preparing papery micro-fluid chip of the present invention, comprise the following steps: the penholder 2 1) material for the formation of microchannel being injected application of sample pen, the described material for the formation of microchannel is the mixture of dimethyl silicone polymer and crosslinking agent, described application of sample pen also comprises nib 1, the front end of nib 1 offers sample tap hole, rotary ball 3 is provided with in sample tap hole, the rear end of nib 1 is connected with penholder 2, after injecting described material, with Seal Oil 6 by material seal in penholder 2, described application of sample pen also comprises the jockey 4 for connecting nib 1 and penholder 2, see Fig. 1, 2) according to the shape application of sample pen of microchannel, described material is write on paper, described writing refers to the surface of end in contact paper before nib 1, then nib 1 is driven to move on paper, the ball 3 of written 1 front end is rotated, the material in penholder 2 is driven to flow out, the material flowed out is coated on paper, can realize micro sample-adding, 3) when material infiltrates after in paper, paper is toasted, makes the material cured in infiltration paper, obtain micro-fluid chip.
The diameter of described ball 2 is 100nm-1cm.The application of sample weight range of described application of sample pen is 10-1000nL/cm.The application of sample amount of application of sample pen is changed by adjustment writing speed (Fig. 5), ball diameter (Fig. 6), penholder inner stuffing height (Fig. 7), writing speed refers to the speed of written front end movement on paper, writing speed can be controlled by driving mechanism, simultaneously, filler supplementary mechanism can be connected in penholder rear end, make the height that penholder inner stuffing keeps stable.
Embodiment
The structure of biological application of sample pen as shown in Figure 1, be made up of 6 parts, nib 1 mainly provides the support point of ball 3, penholder 2 is for stored sample (mixture of dimethyl silicone polymer and crosslinking agent), ball 3 provides enough shearing forces in substrate during frictional rotation, drive sample flows out, and jockey 4 is for connecting penholder 2 and nib 1, and Seal Oil 6 prevents sample from volatilizing.When biological application of sample pen is write in substrate (being similar to the writing process of ball pen or gel ink pen), the ball 3 of written 1 front end rotates, and drives the mixture of dimethyl silicone polymer and crosslinking agent to flow out.
Utilize biological application of sample pen to prepare papery micro-fluid chip, comprise the following steps:
Step 1: the mixture (10 μ L-2mL) of appropriate dimethyl silicone polymer and crosslinking agent is injected penholder, thereafter with mineral oil sealing, avoids evaporating.
Step 2: hold this biological application of sample pen and write on paper, makes the front end in contact paper of nib, and then drive nib to move on paper, the ball of written front end is rotated, drive the mixture of dimethyl silicone polymer and crosslinking agent to flow out, the mixture of outflow is coated on paper.Write line route microchannel is designed and determines.
Step 3: when mixture infiltrates after in paper, paper is put into baking oven and toasts, 80 DEG C are toasted 50 minutes, and object makes dimethyl silicone polymer shaping fixing.
Example 1 utilizes biological application of sample pen to prepare straight channel on paper
Step 1: the mixture of 2mL dimethyl silicone polymer and crosslinking agent (SYLGARD184) is injected penholder, thereafter with mineral oil sealing, avoids evaporating.
Step 2: according to the microchannel shape of design, hold biological application of sample pen and write on paper, writing circuit is straight line.
Step 3: when mixture infiltrates after in paper, paper is put into baking oven and toasts, 80 DEG C are toasted 50 minutes, obtain papery micro-fluid chip, see Fig. 2.
Step 4: add 20 microlitre orchils on papery micro-fluid chip, see Fig. 4 a, after 10 minutes, orchil according to the direction flowing of design, and is dispersed in microchannel, see Fig. 4 b, shows that micro-fluid chip can normally use.
Example 2 utilizes biological application of sample pen to prepare bending channel on paper
Step 1: the mixture of 10 μ L dimethyl silicone polymers and crosslinking agent (SYLGARD184) is injected penholder, thereafter with mineral oil sealing, avoids evaporating.
Step 2: according to the microchannel shape of design, hold biological application of sample pen and write on paper, writing circuit is curve.
Step 3: when mixture infiltrates after in paper, paper is put into baking oven and toasts, 80 DEG C are toasted 50 minutes, obtain papery micro-fluid chip, see Fig. 3.
Claims (4)
1. prepare a method for papery micro-fluid chip, it is characterized in that, comprise the following steps:
1) material for the formation of microchannel is injected the penholder (2) of application of sample pen, described application of sample pen also comprises nib (1), the front end of nib (1) offers sample tap hole, rotary ball (3) is provided with in sample tap hole, the rear end of nib (1) is connected with penholder (2), after injecting described material, with Seal Oil (6) by material seal in penholder (2);
2) according to the shape application of sample pen of microchannel, described material is write on paper;
3) when material infiltrates after in paper, paper is toasted, makes the material cured in infiltration paper, obtain micro-fluid chip;
Described write refer to by nib (1) front end in contact paper surface, then nib (1) is driven to move on paper, the ball (3) of nib (1) front end is rotated, drive the material in penholder (2) to flow out, the material of outflow is coated on paper;
The described material for the formation of microchannel is the mixture of dimethyl silicone polymer and crosslinking agent.
2. a kind of method preparing papery micro-fluid chip according to claim 1, is characterized in that: described application of sample pen also comprises the jockey (4) for connecting nib (1) and penholder (2).
3. a kind of method preparing papery micro-fluid chip according to claim 1, is characterized in that: the diameter of described ball (3) is 100nm-1cm.
4. a kind of method preparing papery micro-fluid chip according to claim 1, is characterized in that: the application of sample weight range of described application of sample pen is 10-1000nL/cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310077796.6A CN103203257B (en) | 2013-03-12 | 2013-03-12 | Method for manufacturing paper micro-fluidic chip |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310077796.6A CN103203257B (en) | 2013-03-12 | 2013-03-12 | Method for manufacturing paper micro-fluidic chip |
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| CN103203257A CN103203257A (en) | 2013-07-17 |
| CN103203257B true CN103203257B (en) | 2015-03-04 |
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| CN201310077796.6A Expired - Fee Related CN103203257B (en) | 2013-03-12 | 2013-03-12 | Method for manufacturing paper micro-fluidic chip |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103394384B (en) * | 2013-08-12 | 2015-07-22 | 广东顺德西安交通大学研究院 | Paper-based microfluidic chip and preparation method thereof |
| CN104209153A (en) * | 2014-09-10 | 2014-12-17 | 南京邮电大学 | Preparation method of paper micro-fluidic chips |
| CN104677896A (en) * | 2015-01-31 | 2015-06-03 | 太原理工大学 | Preparation and application of paper-based microfluidic chip for colorimetric analysis |
| CN106443026A (en) * | 2015-08-10 | 2017-02-22 | 华东理工大学 | Rapid production method of paper-based microfluidic chip |
| CN105903501B (en) * | 2016-04-18 | 2018-12-28 | 南京大学 | A kind of dimethyl silicone polymer-paper compound chip and its preparation method and purposes |
| CN106268990B (en) * | 2016-07-26 | 2018-07-24 | 林捷琳 | A kind of paper micro-flow control chip preparation method based on dot matrix pressure sintering |
| CN106485250A (en) * | 2016-09-09 | 2017-03-08 | 华东理工大学 | A kind of recognition methods of paper chip gray value and its application |
| CN106475157B (en) * | 2016-09-29 | 2019-03-01 | 西安交通大学 | A kind of paper base micro-fluid chip wax crayon device and its application |
| CN109482247B (en) * | 2018-10-18 | 2021-04-13 | 哈尔滨工业大学（深圳） | Micro-fluidic chip manufacturing process and micro-fluidic chip |
| CN113198554A (en) * | 2021-05-14 | 2021-08-03 | 广州大学 | Method for manufacturing micro-fluidic chip formed rapidly on chip |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5911533A (en) * | 1997-11-24 | 1999-06-15 | Eastman Kodak Company | Microfluidic writing pen |
| CN101099683A (en) * | 2003-08-21 | 2008-01-09 | 环球科技（新西兰）有限公司 | Sampling device |
| CN101585507A (en) * | 2009-06-23 | 2009-11-25 | 中国科学院上海微系统与信息技术研究所 | Method for producing through-hole structure in PDMS micro-fluidic chip |
| CN102614948A (en) * | 2012-04-05 | 2012-08-01 | 北京金智捷生物科技有限公司 | Micro-fluidic chip and manufacturing method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RO128117B1 (en) * | 2011-06-16 | 2019-04-30 | Institutul Naţional De Cercetare-Dezvoltare Pentru Microtehnologie | Process of 2d and 3d nanolithography of the optically-assisted fountain pen type |
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2013
- 2013-03-12 CN CN201310077796.6A patent/CN103203257B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5911533A (en) * | 1997-11-24 | 1999-06-15 | Eastman Kodak Company | Microfluidic writing pen |
| CN101099683A (en) * | 2003-08-21 | 2008-01-09 | 环球科技（新西兰）有限公司 | Sampling device |
| CN101585507A (en) * | 2009-06-23 | 2009-11-25 | 中国科学院上海微系统与信息技术研究所 | Method for producing through-hole structure in PDMS micro-fluidic chip |
| CN102614948A (en) * | 2012-04-05 | 2012-08-01 | 北京金智捷生物科技有限公司 | Micro-fluidic chip and manufacturing method thereof |
Non-Patent Citations (1)
| Title |
|---|
| Programmable diagnostic devices made from paper and tape;Andres W.Martinez等;《The Royal Society of Chemistry》;20100730(第10期);第2499-2504页 * |
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| CN103203257A (en) | 2013-07-17 |
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