CN104096609A - Colloidal crystal paper chip and preparation method - Google Patents
Colloidal crystal paper chip and preparation method Download PDFInfo
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- CN104096609A CN104096609A CN201410348630.8A CN201410348630A CN104096609A CN 104096609 A CN104096609 A CN 104096609A CN 201410348630 A CN201410348630 A CN 201410348630A CN 104096609 A CN104096609 A CN 104096609A
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Abstract
The invention discloses a colloidal crystal paper chip and a preparation method thereof. The colloidal crystal paper chip is characterized in that the chip is prepared by construction of hydrophilic and hydrophobic patterns on the surface of a colloidal crystal with a piece of colloidal crystal paper serving as a base chip material. The preparation method comprises the steps as follows: 1, preparing a colloidal crystal film; 2, preparing hydrophilic and hydrophobic channel patterns on the colloidal crystal paper; 3, fixing a chemical color reagent or a biological probe in a detection area. The colloidal crystal paper chip has the advantages that the preparation method is simple and convenient, the porosity is regular and controllable, the separation efficiency is high and the like, can be used for realizing multi-index on-site immediate separation detection and is expected to be applied to the aspects of environmental pollutant detection, rapid food safety detection, biochemical medical detection and the like.
Description
Technical field
The present invention relates to photon crystal material technology, refill chip technology, be specifically related to a kind of colloidal crystal refill sheet and technology of preparing thereof.
Background technology
For adapting to clinical diagnosis and the instant demand detecting, chip technology arises at the historic moment.The importance of lab-on-a-chip and application be by extensive cognition, present chip research mainly concentrate on being easy to get of material, inexpensive, be easy to modify, be easy to store and transport, and refill sheet has well embodied these advantages.Since whitesides group in 2007 proposes the concept of refill sheet for the first time, refill sheet, as a kind of novel detection technique, has demonstrated its huge vitality aspect microminiaturization, portability.Paper can be used as the backing material of separation and Fast Detection Technique, paper using replaces the material sheets such as glass, silicon, quartz, high polymer as substrate, by process parent/hydrophobicity microchannel network and the correlation analysis parts with a fixed structure on paper, the microminiaturization in Realization analysis laboratory, integrated and portability, for each applications such as environmental monitoring, food safety detection, clinical medicine provide new technology platform.But the research of refill sheet is still in initial stage, there are many problems to overcome and to solve.Can the experimental result that obtain as refill sheet and being applied in of having had depend on the voidage of filter paper material used and the size of pore-size distribution and uniformity coefficient to a great extent.Due to the restriction of paper technology at present, commercial filter paper voidage size and pore-size distribution are conventionally wider, within can only being controlled at a certain probable ranges, uniformity is poor, this will affect the uniformity of fluid greatly, in addition the repeatability of filter paper is poor, different batches, and the paper repeatability of different manufacturers is bad.All the separating effect on refill sheet and testing result produce and have larger impact for these.
Colloidal crystal is to assemble by one or more monodispersed colloidal nano particles the class material with two dimension or ordered 3 D structure forming.What in colloidal crystal, occupy each lattice-site is colloidal solid one by one, instead of molecule, atom or the ion in crystal conventionally.Because the long-range order of colloidal particle is arranged, formed regular orderly hole in the inside of colloidal crystal, and the size of these holes can realize by the size of adjusting colloidal nano particle easily.
Therefore, in the present invention, we replace the filter paper using in current refill sheet with colloidal crystal, have prepared a kind of colloidal crystal refill sheet.
Summary of the invention
Technical problem: the object of the present invention is to provide a kind of colloidal crystal refill sheet and technology of preparing, solve voidage and the impact of inequality of pore size on separating effect and detection sensitivity of filter paper in existing refill chip technology.
Technical scheme: in order to solve these problems of the prior art, technical scheme provided by the invention is:
Colloidal crystal refill sheet of the present invention is to obtain taking colloidal crystal paper as base material, and described colloidal crystal paper is the orderly self assembly of colloidal nanoparticles and obtaining, and the particle diameter of described colloidal nanoparticles is between 50nm-10 μ m.
Described colloidal crystal is the one in polystyrene, silica, titanium dioxide, polymethyl methacrylate, polyethyl acrylate or polyethylene.
Described colloidal crystal paper is opal structural, or counter opal structure.
Colloidal crystal refill sheet of the present invention and preparation method preparation method specifically comprise following step:
1) preparation of colloidal crystal paper: single dispersion colloid particle solution uses the method for vertical deposition or electrostatic self-assembled to prepare colloidal crystal paper;
2) on colloidal crystal paper, use photoetching technique, wax seal technology or plasma treatment technology to form the channel network pattern of close and distant water;
3) at the fixing chemochromic reagent of surveyed area or bioprobe, prepare colloidal crystal refill sheet.
The mass percent of described colloidal nanoparticles is between 10%～90%.
Beneficial effect: with respect to scheme of the prior art, advantage of the present invention is:
The present invention, by colloidal crystal paper is replaced to filter paper, successfully realizes control and the optimization of pore size and porosity, has solved fluid consistency problem in existing refill sheet.Chip manufacture method is simple to operate, repeatable high, with low cost.The principles such as chip detection can chemical colour reaction reaction, biological immune reaction, enzymic catalytic reaction or nucleic acid hybridization realize, and therefore have huge application prospect in each fields such as environmental monitoring, food safety detection, clinical medicine.
Brief description of the drawings
Fig. 1 colloidal crystal refill sheet schematic diagram.
Detailed description of the invention
This chip is taking colloidal crystal paper as base material, and described colloidal crystal paper is the orderly self assembly of colloidal nanoparticles and obtaining, and described colloidal crystal paper can be realized by the method such as vertical deposition method, electrostatic self-assembled.Described colloidal crystal paper can be opal structural, or counter opal structure.
Another object of the present invention is to provide a kind of method described in preparation method of colloidal crystal refill sheet to comprise following steps:
(1) preparation of colloidal crystal paper: single dispersion colloid particle solution uses the method for vertical deposition or electrostatic self-assembled to prepare colloidal crystal paper.
(2) on colloidal crystal paper, use the methods such as photoetching technique, wax seal technology or plasma treatment technology to form the channel network pattern of close and distant water.
(3) at the fixing chemochromic reagent of surveyed area or bioprobe (as enzyme, nucleic acid, antigen-antibody etc.), prepare colloidal crystal refill sheet.
Preferably, the particle diameter of the colloidal nanoparticles in described method is between 50nm-10 μ m, and described colloidal nanoparticles is the one in polystyrene, silica, titanium dioxide, polymethyl methacrylate, polyethyl acrylate, polyethylene.
Below in conjunction with specific embodiment, such scheme is described further.Should be understood that these embodiment are not limited to limit the scope of the invention for the present invention is described.The implementation condition adopting in embodiment can be done further adjustment according to the condition of concrete detection, and not marked implementation condition is generally the condition in normal experiment.Any those skilled in the art, without departing from the spirit and scope of the present invention, should make various amendments and change.Therefore protection scope of the present invention should be considered as appending claims limited range.
The preparation of embodiment 1 colloidal crystal refill sheet
(1) preparation of colloidal crystal paper: the silicon dioxide colloid nano particle of monodispersed 500nm is carried out to centrifugal purification, regulating the mass percent of colloidal nanoparticles in colloidal particle solution is 40%, and the surface of glass slide that uses the method for vertical deposition to cross in hydrophilic treated forms uniform colloidal crystal paper.
(2) on colloidal crystal paper, use wax printing technique to form the channel network pattern of close and distant water, heating makes solid wax be melted into Liquid Penetrant in photonic crystal paper, forms hydrophobic barrier, makes the colloidal crystal refill sheet with hydrophilic channel.Drip sample solution at injection port, due to the hydrophily of edge hydrophobic effect and fluid passage, sample solution will enter test point along hydrophilic channel.
(3) in the fixing chemochromic reagent of hydrophily surveyed area, prepare colloidal crystal refill sheet.。
The preparation of embodiment 2 colloidal crystal refill sheets
(1) preparation of colloidal crystal paper: the polystyrene colloid particle of monodispersed 400nm is carried out to centrifugal purification, regulating the mass percent of colloidal nanoparticles in colloidal particle solution is 30%, and the surface of glass slide that uses the method for electrostatic self-assembled to cross in hydrophilic treated forms uniform colloidal crystal paper.
(2) on colloidal crystal paper, use plasma treatment technology to form the channel network pattern of close and distant water, make the colloidal crystal refill sheet with hydrophilic channel.
(3) at hydrophily surveyed area fixed nucleic acid probe, prepare colloidal crystal refill sheet.
The preparation of embodiment 3 colloidal crystal refill sheets
(1) preparation of colloidal crystal paper: the polymethyl methacrylate colloidal particle of monodispersed 1um is carried out to centrifugal purification, regulating the mass percent of colloidal nanoparticles in colloidal particle solution is 20%, and the surface of glass slide that uses the method for vertical deposition to cross in hydrophilic treated forms uniform colloidal crystal paper.
(2) on colloidal crystal paper, use photoetching technique to form the channel network pattern of close and distant water.First optical cement is coated in and on colloidal crystal paper, gets rid of evenly, make filter paper become hydrophobicity, then, under the booster action of mask, utilize uviol lamp to carry out photo-crosslinking polymerization to the optical cement on filter paper, form hydrophobic " dam ".Remove unexposed optical cement, thereby on filter paper, formed close and distant water network channel, make colloidal crystal refill sheet.
(3), at hydrophily surveyed area sessile antibody, prepare colloidal crystal refill sheet.
Claims (5)
1. a colloidal crystal refill sheet, is characterized in that this refill sheet is to obtain taking colloidal crystal paper as base material, and described colloidal crystal paper is the orderly self assembly of colloidal nanoparticles and obtaining, and the particle diameter of described colloidal nanoparticles is between 50nm-10 μ m.
2. a kind of colloidal crystal refill sheet as claimed in claim 1, is characterized in that described colloidal crystal is the one in polystyrene, silica, titanium dioxide, polymethyl methacrylate, polyethyl acrylate or polyethylene.
3. a kind of colloidal crystal refill sheet as claimed in claim 1, is characterized in that described colloidal crystal paper is opal structural, or counter opal structure.
4. colloidal crystal refill sheet as claimed in claim 1 and a preparation method preparation method, is characterized in that this preparation method specifically comprises following step:
1) preparation of colloidal crystal paper: single dispersion colloid particle solution uses the method for vertical deposition or electrostatic self-assembled to prepare colloidal crystal paper;
2) on colloidal crystal paper, use photoetching technique, wax seal technology or plasma treatment technology to form the channel network pattern of close and distant water;
3) at the fixing chemochromic reagent of surveyed area or bioprobe, prepare colloidal crystal refill sheet.
5. the preparation method of colloidal crystal paper as claimed in claim 4, is characterized in that the mass percent of described colloidal nanoparticles is between 10%～90%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410348630.8A CN104096609A (en) | 2014-07-21 | 2014-07-21 | Colloidal crystal paper chip and preparation method |
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| CN201410348630.8A CN104096609A (en) | 2014-07-21 | 2014-07-21 | Colloidal crystal paper chip and preparation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104597237A (en) * | 2015-01-13 | 2015-05-06 | 东南大学 | Photonic crystal-based high-sensitivity immunochromatographic method |
| CN109292816A (en) * | 2018-09-20 | 2019-02-01 | 济南大学 | The preparation method of paper base sea urchin shape titanium dioxide |
| CN111024669A (en) * | 2019-12-27 | 2020-04-17 | 南京工业大学 | Microfluid chip based on multilevel ordered structure photonic crystal paper and preparation method thereof |
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| CN1470319A (en) * | 2003-06-25 | 2004-01-28 | 南京大学 | Capillary attration colloidal microball self-organization and two-dimensional, three-dimensional colloidal crystal preparing method |
| CN1544308A (en) * | 2003-11-24 | 2004-11-10 | 吉林大学 | Soft lithography and tearing technology for colloid crystal microprocessing of pattern |
| CN1710105A (en) * | 2005-06-24 | 2005-12-21 | 东南大学 | Multilayer colloid crystal based biomolecule detecting method |
| CN103879953A (en) * | 2014-04-02 | 2014-06-25 | 东南大学 | Wax-based blade coating patterning method |
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2014
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1470319A (en) * | 2003-06-25 | 2004-01-28 | 南京大学 | Capillary attration colloidal microball self-organization and two-dimensional, three-dimensional colloidal crystal preparing method |
| CN1544308A (en) * | 2003-11-24 | 2004-11-10 | 吉林大学 | Soft lithography and tearing technology for colloid crystal microprocessing of pattern |
| CN1710105A (en) * | 2005-06-24 | 2005-12-21 | 东南大学 | Multilayer colloid crystal based biomolecule detecting method |
| CN103879953A (en) * | 2014-04-02 | 2014-06-25 | 东南大学 | Wax-based blade coating patterning method |
Non-Patent Citations (1)
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| KURT WOSTYN, YUXIA ZHAO, GAETAN DE SCHAETZEN, LOUIS HELLMANS等: "insertion of a two-dimensional cavity into a self-assembled colloidal crystal", 《LANGMUIR》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104597237A (en) * | 2015-01-13 | 2015-05-06 | 东南大学 | Photonic crystal-based high-sensitivity immunochromatographic method |
| CN109292816A (en) * | 2018-09-20 | 2019-02-01 | 济南大学 | The preparation method of paper base sea urchin shape titanium dioxide |
| CN111024669A (en) * | 2019-12-27 | 2020-04-17 | 南京工业大学 | Microfluid chip based on multilevel ordered structure photonic crystal paper and preparation method thereof |
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Application publication date: 20141015 |