CN101839849A - Amylose detector and method for detecting amylose - Google Patents
Amylose detector and method for detecting amylose Download PDFInfo
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- CN101839849A CN101839849A CN 201010136597 CN201010136597A CN101839849A CN 101839849 A CN101839849 A CN 101839849A CN 201010136597 CN201010136597 CN 201010136597 CN 201010136597 A CN201010136597 A CN 201010136597A CN 101839849 A CN101839849 A CN 101839849A
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Abstract
The invention provides an amylose detector, which comprises a laser emitter, a photoelectric detecting unit, a sample injector, an analog input module and a control unit, wherein detection solution after pretreatment is filled in the sample injector; the sample injector converts laser light emitted by the laser emitter into light signals, and transmits the light signals to the photoelectric detecting unit; the photoelectric detecting unit converts the light signals into electric signals and supplies the electric signals to the analog input module which converts the electric signals into digital signals and transmits the digital signals to the control unit; and the control unit calculates the amylose content according to a relation between the absorbance and a detection voltage. The amylose detector provided by the invention uses laser detection, so the accuracy is high, the stability is high, the volume is small and the composition is simple. The invention also provides a method for detecting amylose, which has the advantages of simple steps, short detection time and high accuracy for a detection result.
Description
Technical field
The present invention relates to the detecting instrument field, be specifically related to the detection method of a kind of amylose detector and amylose.
Background technology
Amylose be by glucose with α-1, the chain compound that the 4-glycosidic bond is polymerized can be a maltose by amylorrhexis, the content of amylose in starch is 10%～30%.Can be dissolved in hot water and not become pasty state, meet iodine and show blue.Amylose is the main nutrient composition of crops such as wheat, corn, rice, jowar, potato, and the key factor that how much influences food consumption quality and technological quality of its content also is simultaneously that the evaluation grain economy is worth one of important indicator of height.
(Amylose Content AC) is one of greatest factor of influencing rice cooking and processing characteristics to the amylose content of rice.Show as behind the low rice cooking of amylose content that viscosity is big, rice is soft and glossy, and can receive more water during the high rice cooking of amylose content and constantly expand, rice grain drying, fluffy and look dark.Amylose content is very big to the quality influence of rice noodles, and with the long-grained nonglutinous rice processing rice noodles of amylose content 18%～24%, operation is smooth, and rice noodles are pliable and tough smooth, and certain elasticity is arranged, and color and luster is good, and organoleptic quality is better.Amylose content is high more, and the rice noodles quality is good more, but amylose content gelatinization difficulty that becomes when too high.Amylose content is lower than 18%, and the rice noodles organoleptic quality is poor, and adding man-hour even can wire vent, and adherent phenomenon is more serious.Amylose content has significant effects to the rice made products exterior quality, and the food that the rice that amylose content is low is made is glittering and translucent, and color and luster is beautiful.And the food that the high rice of amylose content is made is intensely dark, and sense organ is bad.
The wheat amylose content has significant effects for numerous characteristics such as the crystallization of starch, viscosity, degree of shear.Wheat breed that amylose content is lower or flour have performance preferably on Q factors such as noodles softness, viscosity, slickness, mouthfeel and comprehensive grading.Little, the poor toughness of the steamed bun volume that the wheat flour that amylose content is high is made, the noodles of making are easily broken, and steamed bun that the flour that amylose content is moderate or on the low side is made and noodles have toughness and edible quality preferably.
The corn amylose is one of human main amylose source, is industrial amylose and is the primary raw material of the deep processing industry of raw material with the amylose.Be applied to more than 30 field, as industries such as paper, packing, oil, environmental protection, optical fiber, high precision printed-wiring board (PWB), electronic chips.
Two kinds of main colourimetry of method that amylose detects and near infrared spectroscopies.Colourimetry is to detect the classical approach of amylose content, is the reference method of most countries standard, though measurement result accurately and reliably, detection time is longer.Though it is fast that near-infrared method is measured speed, harmless to sample, need set up calibration model, need to obtain accurate values with reference to authoritative accredited laboratory standard value.Use the colorimetric method for determining amylose content, external starting early, as far back as 1958, Williams has just proposed to use the colorimetric method for determining rice grain amylose content, business-like instrument has been arranged at present, amylose automated analysis instrument FLAstar 5000 Analyzer of Sweden Foss company for example, the FS-IV chemistry automatic analyzer of U.S. O-I company.But these instruments cost an arm and a leg, and are difficult to promote the use of at home.And on the domestic research level that also rests on the scientific research model machine, there is not business-like amylose pick-up unit.Near infrared spectroscopy is measured amylose, external starting also early, business-like instrument is also arranged at present, Fourier's near infrared spectrometer of for example German Bruke company, the rice quality near infrared spectrometer of Sweden FOSS company, the near infrared light spectrum model of these instruments can not adapt to many kinds cereal type requirement of China, still is on the algorithm research level at present.
The instrument of domestic mensuration amylose mainly is the ultraviolet-visible spectrophotometer, and its principle is: starch and iodine form the iodine-starch compound, and have special color reaction.Amylopectin and iodine generate the brownish red compound, and amylose and iodine generate the mazarine compound.Under the constant condition of starch total amount, these two kinds of starch dispersion liquids are pressed different proportion to be mixed, under the condition of certain wavelength and acidity with the iodine effect, generation by purplish red to dark blue a series of colors, represent its different amyloses and amylopectin content ratio, according to Lambert-Beer's law, absorbance and amylose concentration are linear, available spectrophotometry.
The monochromatic light that the ultraviolet-visible spectrophotometer needs is obtained by the monochromator decomposition by the mixed light that halogen tungsten lamp (wavelength coverage is 320-1100nm) sends, and there is the contradiction between purity and the intensity in the monochromatic light that obtains like this.If the assurance light intensity, monochromatic light is impure, thereby nonmonochromatic light causes departing from of Lambert-Beer's law caused measuring error.If guarantee the purity of light, light intensity is not enough, and light penetrates the ability of solution, causes absorbance to diminish, and by transmittance error of indication theory as can be known, if absorbance is very little, measuring error is just very big.And ultraviolet-visible spectrophotometer automaticity is low, and a lot of links of operating process need rely on operating personnel's analysis experience to be implemented, and unavoidably produce the manual operation error.
Summary of the invention
The technical problem to be solved in the present invention is, a kind of amylose detector is provided, and easy to operate, volume is little, and the precision high stability is good; A kind of detection method of amylose, it is simple to detect step, and detection time is short, testing result precision height.
In order to overcome the above problems, the invention provides a kind of amylose detector, it is characterized in that, comprising: generating laser, photodetector unit, injector, analog input module and control module;
Wherein: the solution that pretreated detection is housed in the described injector;
Described injector is converted to light signal with the laser that described generating laser sends, and described light signal is delivered to described photodetector unit;
Described optical detecting unit offers described analog input module after described light signal is converted to electric signal, sends to described control module after being converted to digital signal by described analog input module;
Described control module is according to the relational expression between absorbance and the detection voltage
Calculate amylose content; Wherein A is an absorbance, u
0Be normal voltage, u is for detecting voltage.
Preferably, described photodetector unit is specially: photoelectric detective circuit and be installed in photoelectric cell on the described photoelectric detective circuit; Light signal is converted into current signal by photoelectric cell and is transferred to photoelectric detective circuit.
Preferably, described photoelectric detective circuit is specially: low input impedance current-voltage conversion circuit A
1And second amplifying circuit A
2Described A
1With described A
2Between the series connection one resistance R; A
1Current signal is converted to voltage signal sends to A
2, A
2Described voltage signal amplified and send to analog input module.
Preferably, described injector is specially: cuvette, peristaltic pump, tail pipe and draft tube, the solution that described peristaltic pump will need to detect is sent in the described cuvette by described tail pipe, behind the solution absorption portion laser in the described cuvette and will remain laser-transmitting to described photodetector unit, described peristaltic pump with the solution in the described cuvette by described draft tube sucking-off.
Preferably, described Wavelength of Laser is 600nm～650nm.
Preferably, the light path of described cuvette is 0.5cm～1.5cm.
A kind of detection method of amylose comprises:
A) will detect solution and carry out pre-service;
B) use the described detection solution of laser radiation, obtain light signal;
C) described light signal is converted into current signal, and then current signal is converted into voltage signal;
D) described voltage signal is obtained digital signal by mould/number conversion;
E) repeating step b)～d) detect mixed solution, the product to be tested solution of blank assay solution, amylose standard and amylopectin standard solution successively, and by the relational expression between absorbance and the detection voltage
Calculate amylose content; Wherein A is that A is an absorbance, u
0Be normal voltage, u is for detecting voltage.
Preferably, the detection solution described in the step a) is blank test solution, standard starch solution, testing sample solution.
Preferably, step a) is specially: the NaOH solution of a1) getting concentration and be 0.09mol/L mixes described blank test solution as blank test solution with distilled water, acetic acid, iodine reagent, is settled to the 10min that develops the color behind the 100mL with distilled water;
A2) concentration of getting 5～8 groups of different volumes respectively is that standard straight chain starch solution and the standard pullulan solution of 1mg/mL mixes, all obtain 5mL standard starch solution, described standard starch solution is mixed with distilled water, acetic acid, iodine reagent, be settled to the 10min that develops the color behind the 100mL with distilled water;
A3) with thick starch and absolute ethyl alcohol and 9mL concentration for the 1mol/L sodium hydroxide solution mixes, obtain the starch mixed liquor, described starch mixed liquor is disperseed to obtain dispersion liquid after the 10min cooling in boiling water bath;
Described dispersion liquid is mixed with sherwood oil, obtain the sherwood oil mixed liquor, leave standstill 10min～20min, petroleum ether layer is removed in the layering of described sherwood oil mixed liquor, obtains testing sample solution;
Described testing sample solution is mixed with distilled water, acetic acid, iodine reagent, be settled to the 10min that develops the color behind the 100mL with distilled water.
Preferably, step e) is specially: e1) repeating step b)～d) detect blank assay solution, standard starch solution, product to be tested solution respectively and obtain blank assay voltage u
0, standard test voltage u
11～u
16With product to be tested solution voltage u
2
E2) described control module is according to absorbance
The absorbance that calculates amylose standard test and amylopectin standard test is also by known standard absorbance match typical curve;
E3) utilize u again
11～u
16And u
2Calculate the absorbance of product to be tested, bring into and calculate and export amylose content to be tasted with discrimination in the described typical curve.
The invention provides a kind of amylose detector, comprising: generating laser, photodetector unit, injector, analog input module and control module; Wherein: the solution that pretreated detection is housed in the described injector; Described injector is converted to light signal with the laser that described generating laser sends, and described light signal is delivered to described photodetector unit; Described optical detecting unit offers described analog input module after described light signal is converted to electric signal, sends to described control module after being converted to digital signal by described analog input module; Described control module is according to the relational expression between absorbance and the detection voltage
Calculate amylose content; Wherein A is an absorbance, u
0Be normal voltage, u is for detecting voltage.Amylose detector provided by the invention uses laser detection, and the precision high stability is good, and it is simple that instrument volume group becomes.The present invention also provides a kind of detection method of amylose, comprising: a) will detect solution and carry out pre-service; B) use the described detection solution of laser radiation, obtain light signal; C) described light signal is converted into current signal, and then current signal is converted into voltage signal; D) described voltage signal is obtained digital signal by mould/number conversion; E) repeating step b)～d) detect mixed solution, the product to be tested solution of blank assay solution, amylose standard and amylopectin standard solution successively, and by the relational expression between absorbance and the detection voltage
Calculate amylose content; Wherein A is that A is an absorbance, u
0Be normal voltage, u is for detecting voltage.This method step is simple, and detection time is short, testing result precision height.
Description of drawings
Fig. 1 amylose detector provided by the invention synoptic diagram;
Fig. 2 amylose detector provided by the invention photoelectric detective circuit figure;
Fig. 3 amylose testing process provided by the invention figure;
The affected window of Fig. 4 amylose detector provided by the invention software;
Fig. 5 amylose detector provided by the invention software control platform window;
Fig. 6 amylose detector provided by the invention software standard specimen is set window;
Fig. 7 amylose detector provided by the invention software standard specimen information display window;
Fig. 8 amylose detector provided by the invention software testing sample quality settings window;
Fig. 9 amylose detector provided by the invention software testing sample information display window.
Embodiment
In order further to understand the present invention, below in conjunction with embodiment the preferred embodiments of the invention are described, but should be appreciated that just restriction of these descriptions for further specifying the features and advantages of the present invention rather than patent of the present invention being required.
The invention provides a kind of amylose detector, comprising: generating laser, photodetector unit, injector, analog input module, control module;
Wherein: the solution that pretreated detection is housed in the described injector;
Described injector is converted to light signal with the laser that described generating laser sends, and described light signal is delivered to described photodetector unit;
Described optical detecting unit offers described analog input module after described light signal is converted to electric signal, sends to described control module after being converted to digital signal by described analog input module;
Described control module is according to the relational expression between absorbance and the detection voltage
Calculate amylose content, wherein A is an absorbance, u
0Be normal voltage, u is for detecting voltage.
Starch detector provided by the invention according to principle be: starch and iodine form the iodine-starch compound, can have special color reaction.Amylopectin and iodine generate the brownish red compound, and amylose and iodine generate the mazarine compound.Under the constant condition of starch total amount, these two kinds of starch dispersion liquids are pressed different proportion to be mixed, under the condition of certain wavelength and acidity with the iodine effect, generation by purplish red to dark blue a series of colors, represent its different amyloses and amylopectin content ratio, linear according to absorbance and amylose concentration, the content of mensuration amylose.
According to the present invention, as shown in Figure 1,1 is that generating laser, 2 is that cuvette, 3 is that photoelectric detective circuit, 4 is that photoelectric cell, 5 is that tail pipe, 6 is that draft tube, 7 is that peristaltic pump, 8 is that analog input module, 9 is a control module.
Generating laser provided by the invention is a generating laser well known in the art, emitted laser is the single light beam of concentration of energy and color, can not cause the phenomenon that is inversely proportional between optical purity and the light intensity, the quality of having stablized light signal has also been stablized the quality of final detection result, the wavelength that the present invention uses is the laser of 600nm～650nm, laser in this wavelength coverage can be good at absorbing for middle starch solution part, thereby records accurate data more.
Injector provided by the invention is specially cuvette 2, tail pipe 5, attract deposit pipe 6, peristaltic pump 7, before detector begins test sample, peristaltic pump is provided with single-chip microcomputer can receive the startup of enabled instruction control peristaltic pump, by the velocity of rotation and the sample injection time of Single-chip Controlling pump, the LED charactron shows sampling volume.The panel that carries three buttons that open/stop, just change, reverse.Has the outer control interface that intelligent control is provided.From three buttons of its panel, the 7012 digital quantity I/O mouths that go between, control module can be controlled the I/O mouth by output order and realize opening/stop, just change, reversing of Based Intelligent Control pump.Just changeing exactly that wherein the solution in the peristaltic pump is entered into cuvette by tail pipe, counter-rotating then is that the solution in the cuvette flows back to peristaltic pump by draft tube.Cuvette light path provided by the invention is preferably 0.5cm～1.5cm, more preferably 0.8cm～1.2cm.
Photodetector unit provided by the invention specifically comprises photoelectric detective circuit and photoelectric cell, and wherein photoelectric cell is preferably the photoelectric conversion efficiency of silicon photovoltaic cell height, and the linearity of illumination characteristic is good, the response time of silicon photocell is 10.3s～10.6s.Spectral response range is 400nm-1100nm.Photodetector unit provided by the invention has been utilized silicon photocell relatively to be fit to receive visible light and has been had short-circuit current with the linear characteristic that increases of intensity of illumination, can more accurate conversion light signal.Linear within the specific limits between photronic short-circuit current and the incident intensity, promptly current signal is directly proportional with solution absorbency, and in photoelectric detective circuit, photoelectric cell inserts with the form of current source.Because current signal is very faint, select for use two-stage chopper-zero-stabilized integrated operational amplifier ICL7650 to form testing circuit.First order amplifying circuit A
1Be a low input impedance current-voltage conversion circuit, make silicon blue cell be operated in the electric current output area, have the better linearity relation, with the voltage signal of weak current signal transition for being directly proportional with it; Second level amplifying circuit A
2Voltage signal is amplified, make amplitude output signal between 0～5V.Fit adjustment 5K, the potentiometer of 50K makes testing circuit stablize the voltage of output 0～5V.
Analog input 7012 modules provided by the invention are data processing well known in the art and D/A switch module, voltage signal can be converted to the control module code and export to control module by corresponding serial ports, make the definite conversion of data integrity.Control module provided by the invention is preferably computing machine, because of it has powerful data processing and management control function, each step of control detection immediately, and with each parts of amylose detector unifiedly operate, management and reducing the detection step, time saving and energy saving, finally pass through
Calculate absorbance.
Amylose detector provided by the invention in use, to join in the peristaltic pump after the starch solution processing, start the peristaltic pump switch, in LCD display, observe the liquid level and the Control Flow of peristaltic pump, input open detection order in computing machine, the computer Recognition order also is transferred to analog input module, it receives the order back and judges order source and intention, and with command transfer to the single-chip microcomputer on the peristaltic pump, the Single-chip Controlling peristaltic pump is started working, and starch solution is injected cuvette.Open generating laser, adjust optical maser wavelength, laser passes two sidewalls of cuvette, penetrate on photoelectric cell, at this moment light signal can change into current signal at inside battery, and by photoelectric detective circuit current signal is converted into the voltage model, and signal can carry out D/A switch in analog input module then, transmit back computing machine via serial ports again, the data that computing machine is passed back according to analog input module are judged type and according to formula
Come the drawing curve, and calculation of starch content.
The invention provides a kind of amylose detection method, comprising: a) will detect solution and carry out pre-service;
B) use the described detection solution of laser radiation, obtain light signal;
C) described light signal is converted into current signal, and then current signal is converted into voltage signal;
D) described voltage signal is obtained digital signal by mould/number conversion;
E) repeating step b)～d) detect blank assay solution, amylose standard solution, amylopectin standard solution, product to be tested solution successively, and by the relational expression between absorbance and the detection voltage
Calculate amylose content; Wherein A is an absorbance, u
0Be normal voltage, u is for detecting voltage.
The detection method of amylose provided by the invention is based on amylose detector provided by the invention and implements, its principle is to utilize the light intensity that can absorb after the starch solution colour developing of different amylose contents also different, utilize laser to pass light signal behind the starch solution of colour developing like this through a series of transformation, obtain voltage signal, pass through formula again
Calculate the absorbance A of starch solution, again by with the contrast of standard working curve, obtain changing the content of the middle amylose of starch solution.
According to the present invention, at first prepare amylose standard solution, amylopectin standard solution, testing sample solution.Preparation method according to each solution of the present invention is:
At first demarcating the sodium hydroxide solution of 1mol/L and the acetum of 1mol/L is placed in the volumetric flask.Weighing then is 1: 10 iodine and potassium iodide solid, with dissolved in distilled water and rare analysing to 100mL, as the iodine storing solution.It is rare to 100mL to get 10mL iodine storing solution, as iodine reagent.
Demarcate the NaOH solution of 0.09mol/L then.
Prepare the amylose standard solution again, take by weighing weight 0.1000g purity and be 100% amylose, put into the 100mL volumetric flask, add the moistening sample of 1mL absolute ethyl alcohol, the sodium hydroxide solution that adds 9mL 1mol/L again disperses 10min in boiling water bath, after cooling off rapidly, and water constant volume 100mL.
Preparation amylose standard solution takes by weighing weight 0.1000g purity and is 100% amylopectin, puts into the 100mL volumetric flask, add the moistening sample of 1mL absolute ethyl alcohol, the sodium hydroxide solution that adds 9mL1mol/L again heats 10min in boiling water bath, after cooling off rapidly, and water constant volume 100mL.
Prepare testing sample solution at last, after testing sample sieved with 80 mesh sieve, take by weighing testing sample 0.1000g, put into the 100mL volumetric flask, add the moistening sample of 1mL absolute ethyl alcohol, the sodium hydroxide solution that adds 9mL 1mol/L again disperses 10min in boiling water bath, after cooling off rapidly, and water constant volume 100mL.
After each formulations prepared from solutions is finished, in order to reach the purpose of detection, at first to carry out baseline calibration to detector, to prepare blank detect solution and colour developing during calibration, the blank test of advancing the amylose detector detects and obtains baseline, then the amylose standard solution is mixed standard detection solution and the colour developing that obtains 6 different proportionings with the amylopectin standard solution according to proportioning, these 6 different standard detection solution are detected in the amylose detector, obtain data and draw 6 standard working curves.And then with the colour developing of product to be tested solution, it is detected in the amylose detector, the data that obtain compare the amylose content result who obtains product to be tested by Computer Processing and standard working curve.The solution that is about to configure according to the so-called colour developing of the present invention mixes and keeps 10min with acetum, iodine reagent, and solution shows the process of the reddish brown a series of colors between royal purple with feature.
The blank process that detects solution preparation and colour developing is specially: blank detects in the preparation and the volumetric flask of pre-service according to sodium hydroxide solution to a 100mL who gets 5mL0.9mol/L of solution, get acetum, the 1mL iodine reagent of 50mL distilled water, 1mL1mol/L successively, be settled to the 10min that develops the color behind the 100mL with distilled water.
The preparation of standard detection solution and process color are: get 6 100mL volumetric flasks, add 1mg/mL potato amylose standard solution 0,0.25,0.50,1.00,1.50,2.00mL respectively, add amylopectin standard solution 5,4.75,4.50,4.00,3.50,3.00mL more successively, total amount is 5mL.Other gets the volumetric flask of 1 100mL, adds 0.09mol/L sodium hydroxide solution 5mL and does blank.The acetate and the 1mL iodine reagent that in each bottle, add about 50mL water, 1mL1mol/L then successively.10min develops the color behind the water constant volume.
The color development treatment of product to be tested solution is: take by weighing the thick samples with starch of 0.1000g in the 100mL volumetric flask, add the 1mL absolute ethyl alcohol, abundant moistening sample adds the 9mL1mol/L sodium hydroxide solution again and disperse 10min in boiling water bath, cooling rapidly, water constant volume; Get the 20mL dispersion liquid and possess in the scale test tube in 50mL, add 7mL～10mL petroleum chain, intermittently shake 10min, static 15min with the suction pipe suction that is connected on the water pump, inhales and removes the top petroleum ether layer after the layering, repeats above operation 2～3 times; Get testing sample solution 5mL, put into the volumetric flask of 100mL.The acetic acid, the 1mL iodine reagent that add 50mL distilled water, 1mL1mol/L successively.Be settled to the 10min that develops the color behind the 100mL with distilled water.
Be divided into 3 stages according to testing process of the present invention, phase one is a baseline calibration, promptly blank the detection, the blank that colour developing is good detects solution and adds in the peristaltic pump, serial ports in window shown in Figure 4 selects to select in the option serial ports 1, select baseline calibration in the control desk, click then and determine, computing machine sends startup command and is transferred to analog input module by serial ports 1, after analyzing data through it, startup command is transferred to single-chip microcomputer on the peristaltic pump, single-chip microcomputer judges that the source of startup command begins to start peristaltic pump, blank is detected solution inject cuvette by tail pipe, open generating laser, adjust its emission Wavelength of Laser between 600nm～650nm, laser passes cuvette and penetrates on silicon photocell, begin a series of conversion of signals, obtain a voltage signal u at last
0Be transferred to analog input module by photoelectric detective circuit, by it voltage signal is carried out D/A switch and transmit back computing machine by serial ports 1 again, this is that data processing unit in the computing machine is analyzed the data that change voltage signal, and in Fig. 4 the left window Calibration Base Line, and preserve data.
Subordinate phase is the drafting of standard working curve, in the control desk of Fig. 4 window, select standard specimen to detect, in standard specimen numbering dialog box, fill in standard detection solution numbering then, the content of filling in the amylose in the standard detection solution in the amylose content dialog box as shown in Figure 6, click then and determine, computer control detects and starts and begin to detect 6 groups of standard detection solution according to the phase one step, obtains voltage u
11～u
16, then according to the relational expression that detects voltage and absorbance
Calculate 6 groups of standard detection solution absorbency A
1～A
6, can obtain standard working curve in Fig. 4 window left side according to known amylose content again.Wherein the x axle is an absorbance, and the y axle is an amylose content.Obtain saving result behind the standard working curve, computing machine can automatic store results.If the standard working curve result is dissatisfied or because operational issue has caused error, can delete measurement result by the deletion standard specimen option in the control desk, be noted that, do not test one group of standard detection solution and all want observations whether problem is arranged, if the result has problem in time deletion and duplicate detection.According to the present invention, the testing result of standard detection solution as shown in Figure 7.
Phase III is the detection of testing sample, in the control desk of Fig. 4 window, select sample detection, in ejecting the sample number into spectrum dialog box, fill in numbering then, in the sample quality dialog box, fill in the testing sample quality as shown in Figure 8, click then and determine, computer control detects and starts and begin to detect testing sample solution according to the phase one step, obtains voltage u, and according to the relational expression that detects voltage and absorbance
Obtain the absorbance A of testing sample, can draw the content of the amylose in the testing sample according to its position in standard working curve, the result as shown in Figure 9.
The invention provides a kind of amylose detector, it is characterized in that, comprising: generating laser, photodetector unit, injector, analog input module and control module; Wherein: the solution that pretreated detection is housed in the described injector; Described injector is converted to light signal with the laser that described generating laser sends, and described light signal is delivered to described photodetector unit; Described optical detecting unit offers described analog input module after described light signal is converted to electric signal, sends to described control module after being converted to digital signal by described analog input module; Described control module is according to the relational expression between absorbance and the detection voltage
Calculate amylose content; Wherein A is an absorbance, u
0Be normal voltage, u is for detecting voltage.Amylose detector provided by the invention uses laser detection, and the precision high stability is good, and it is simple that instrument volume group becomes.The present invention also provides a kind of detection method of amylose, comprising: a) will detect solution and carry out pre-service; B) use the described detection solution of laser radiation, obtain light signal; C) described light signal is converted into current signal, and then current signal is converted into voltage signal; D) described voltage signal is obtained digital signal by mould/number conversion; E) repeating step b)～d) detect mixed solution, the product to be tested solution of blank assay solution, amylose standard and amylopectin standard solution successively, and by the relational expression between absorbance and the detection voltage
Calculate amylose content; Wherein A is that A is an absorbance, u
0Be normal voltage, u is for detecting voltage.This method step is simple, and detection time is short, testing result precision height.
More than a kind of amylose detector provided by the invention and amylose detection method are described in detail; having used specific case herein sets forth principle of the present invention and embodiment; the explanation of above embodiment just is used for helping to understand method of the present invention and core concept thereof; should be understood that; for those skilled in the art; under the prerequisite that does not break away from the principle of the invention; can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of claim of the present invention.
Claims (10)
1. an amylose detector is characterized in that, comprising: generating laser, photodetector unit, injector, analog input module and control module;
Wherein: the solution that pretreated detection is housed in the described injector;
Described injector is converted to light signal with the laser that described generating laser sends, and described light signal is delivered to described photodetector unit;
Described optical detecting unit offers described analog input module after described light signal is converted to electric signal, sends to described control module after being converted to digital signal by described analog input module;
Described control module is according to the relational expression between absorbance and the detection voltage
Calculate amylose content; Wherein A is an absorbance, u
0Be normal voltage, u is for detecting voltage.
2. amylose detector according to claim 1 is characterized in that, described photodetector unit is specially: photoelectric detective circuit and be installed in photoelectric cell on the described photoelectric detective circuit; Light signal is converted into current signal by photoelectric cell and is transferred to photoelectric detective circuit.
3. amylose detector according to claim 2 is characterized in that, described photoelectric detective circuit is specially: low input impedance current-voltage conversion circuit A
1And second amplifying circuit A
2Described A
1With described A
2Between the series connection one resistance R; A
1Current signal is converted to voltage signal sends to A
2, A
2Described voltage signal amplified and send to analog input module.
4. amylose detector according to claim 1, it is characterized in that, described injector is specially: cuvette, peristaltic pump, tail pipe and draft tube, the solution that described peristaltic pump will need to detect is sent in the described cuvette by described tail pipe, behind the solution absorption portion laser in the described cuvette and will remain laser-transmitting to described photodetector unit, described peristaltic pump with the solution in the described cuvette by described draft tube sucking-off.
5. amylose detector according to claim 4 is characterized in that, described Wavelength of Laser is 600nm～650nm.
6. amylose detector according to claim 5 is characterized in that, the light path of described cuvette is 0.5cm～1.5cm.
7. the detection method of an amylose is characterized in that, comprising:
A) will detect solution and carry out pre-service;
B) use the described detection solution of laser radiation, obtain light signal;
C) described light signal is converted into current signal, and then current signal is converted into voltage signal;
D) described voltage signal is obtained digital signal by mould/number conversion;
E) repeating step b)～d) detect mixed solution, the product to be tested solution of blank assay solution, amylose standard and amylopectin standard solution successively, and by the relational expression between absorbance and the detection voltage
Calculate amylose content; Wherein A is that A is an absorbance, u
0Be normal voltage, u is for detecting voltage.
8. detection method according to claim 7 is characterized in that, the detection solution described in the step a) is blank test solution, standard starch solution, testing sample solution.
9. detection method according to claim 7, it is characterized in that, step a) is specially: the NaOH solution of a1) getting concentration and be 0.09mol/L mixes described blank test solution as blank test solution with distilled water, acetic acid, iodine reagent, is settled to the 10min that develops the color behind the 100mL with distilled water;
A2) concentration of getting 5～8 groups of different volumes respectively is that standard straight chain starch solution and the standard pullulan solution of 1mg/mL mixes, all obtain 5mL standard starch solution, described standard starch solution is mixed with distilled water, acetic acid, iodine reagent, be settled to the 10min that develops the color behind the 100mL with distilled water;
A3) with thick starch and absolute ethyl alcohol and 9mL concentration for the 1mol/L sodium hydroxide solution mixes, obtain the starch mixed liquor, described starch mixed liquor is disperseed to obtain dispersion liquid after the 10min cooling in boiling water bath;
Described dispersion liquid is mixed with sherwood oil, obtain the sherwood oil mixed liquor, leave standstill 10min～20min, petroleum ether layer is removed in the layering of described sherwood oil mixed liquor, obtains testing sample solution;
Described testing sample solution is mixed with distilled water, acetic acid, iodine reagent, be settled to the 10min that develops the color behind the 100mL with distilled water.
10. detection method according to claim 7 is characterized in that step h) be specially: e1) repeating step b)～d) detect blank assay solution, standard starch solution, product to be tested solution respectively and obtain blank assay voltage u
0, standard test voltage u
11～u
16With product to be tested solution voltage u
2
E2) described control module is according to absorbance
The absorbance that calculates amylose standard test and amylopectin standard test is also by known standard absorbance match typical curve;
E3) utilize u again
0And u
2Calculate the absorbance of product to be tested, bring into and calculate and export amylose content to be tasted with discrimination in the described typical curve.
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