CN102279263B - CCD-type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers - Google Patents
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Abstract
The invention relates to a CCD-type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers and an analysis method. According to the invention, a CCD digital image technology is used for carrying out instant shooting, processing and reading upon tested targets. The system comprises an image collecting device, a data processing device, and a result outputting device. Bilateral handshaking is respectively carried out between a motherboard handshaking terminal and image processing and reading software, and between a motherboard handshaking terminal and an industrial camera in the image collecting device. The system and the method are advantaged in that: an illumination light source with a patent technology, a self calibrate algorithm, and an image noise reduction algorithm are adopted, such that the stability, consistency and accuracy of reading results can be effectively ensured, and subjective deviation caused by human eye interpretation can be solved; an integral industrial control computer application architecture, with which the invention applied for an independent intellectual property right, is integrated in the instrument system; the parts such as modularized LED illumination, CCD camera, data processing system, can be selected according to user requirements of different performances and prices; according to the invention, parts of the software are developed on a mainstream Windows platform, such that subsequent function upgrading and expanding can be promoted.
Description
Technical field
The present invention relates to a kind of CCD of employing Digital image technology to detected target immediately take, a kind of CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers and the analytical approach of processing and reading, be applicable to the quick judgement of early pregnancy, influenza, drugs isocolloid gold immunochromatography diagnose test paper result.
Background technology
At present, on market, there is multiple colloidal gold immunochromatographimethod diagnose test paper analytic system or instrument.Analogous instrument or equipment that foreign vendor releases as GEM Premier, precision is high, good stability, easy to use.But be only applicable to the test strips product test strips bundle sale of oneself production of manufacturer (many with) of particular type.The i-CHROMA Reader immunofluorescence analysis instrument of Korea S, detection sensitivity can reach pg/ml, but the price of a cover system exceedes 100,000 Renminbi.
What domestic manufacturer early released adopts the quantitative gold-marking immunity analyser of wavelength specific laser as light source in addition, as the DJM series of products of Shanghai Daheng Optical Fine Mechinery Co., Ltd's release, its reading accuracy is higher, but range of readings is narrow, can only be used for the test strips of particular type (color).
Adopt the golden scalar quantity detector of tintmeter principle, as the HCG-DOT of Shanghai Ao Pu biological medicine company limited production.When each start work, need manual operation Standard colour board to carry out system calibration; In addition, adopt photodiode as signals collecting parts, cannot carry out accurate quantitative test, and the test strips product that can only use this producer to provide.
Summary of the invention
Purpose of design: avoid the weak point in background technology, design a kind of CCD of employing Digital image technology to detected target immediately take, a kind of CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers and the analytical approach of processing and reading.
Design proposal: in order to realize above-mentioned purpose of design.The application is according to the market demand, and after deep feasibility analysis, independent development " CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers ".This system adopts digital imaging technology, absorption photometry principle, by light source design, self calibration and the numerical value filter algorithm of patented technology, has solved a difficult problem of colloidal gold immunochromatographimethod diagnose test paper being carried out to quantitative test.When system works, first reacted colloidal gold immunochromatographimethod diagnose test paper is sent in the target pallet of instrument fixing; The bright test strips of the steady illumination illumination surface being provided by LED illuminating chamber, reflected light converts digital picture to through image acquisition component; Finally, software, according to the digital picture obtaining, calculates the respective value of the p-wire in test strips.
1, the instrument in the application has mainly adopted absorption photometry principle, and the reacted test strips of immunochromatography is carried out to reading.Introduce its principle below.
2, the application adopts absorption photometry: absorption photometry is the absorbance by measuring solution, determines the concentration of substance solution according to lambert's one law of Beer.
1760, Lambert pointed out with experiment, and when light is when the transparent medium, the degree that weakens of light is directly proportional by the light path of medium to light.1852, Beer studies have shown that, the number of the extinction particle that the degree of absorption of light runs into light in transparent medium is directly proportional, and in solution, is directly proportional to the concentration of extinction particle.
Absorbance:
Transmittance:
3, immunochromatography reaction: the structure of colloidal gold immunochromatographimethod diagnose test paper mainly comprises sample pad, pad, analyzing film, p-wire, thing nature controlling line, adsorptive pads.Wherein, nanogold particle is combined and is fixed in pad with bioactive molecule.As shown in Figure 3.
In the time detecting, first the sample drop that contains target checking matter is added in sample pad, sample enters pad by infiltration and syphonic effect, and the analyzing film of flowing through, flows to adsorptive pads.In this process, on analyzing film p-wire and nature controlling line, complicated immune response will be there is.
After immunochromatography reaction finishes, on p-wire and nature controlling line, be all fixed with nanogold particle, and on p-wire in the content of nanogold particle and sample the concentration of target checking matter have corresponding relation.If not containing target checking matter, just there is no combining nano gold grain on p-wire in sample.
4, system forms: " CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers " is made up of image acquisition device, data processor, result follower; Wherein, image acquisition device is made up of target pallet, LED illuminating chamber, optical flat catoptron, industrial camera; Data processor is processed with reading software and is formed by motherboard, image; Result follower is made up of mini-printer, liquid crystal display, as described in Figure 1.Instrument working parameter mainly comprises: image acquisition control parameter, reading Analysis parameter and calibration parameter.Image acquisition control parameter mainly contains time shutter, contrast, white balance, noise reduction number of samples etc.; Reading reading Analysis parameter mainly contains the setting of reading region, RGB triple channel weighted value, the setting of data effective range etc.; Calibration parameter has gain, side-play amount etc.Instrument readings result is the p-wire on colloidal gold immunochromatographimethod diagnose test paper, the numeric representation of nature controlling line.In the time of instrument long-play, the operating characteristic of lighting source and image acquisition component is inevitably for example, by the variation occurring to a certain degree, brightness; In addition, image acquisition component, can be with the noise (hot-tempered point) of some in the digital picture obtaining due to the work characteristics of electronic imaging system.Test datas show in a large number, and this class variation can finally have influence on reading result.For weakening or eliminating this class and disturb, this project Instrument Design brightness self-calibration function, image multiframe decrease of noise functions.The corresponding running parameter such as brightness locking reference value, noise reduction number of samples can be by user according to actual needs, arranges and adjust, to realize the reliable and stable of instrument readings result.
The colloidal gold immunochromatographimethod diagnose test paper that different size or different manufacturers are produced, color, lines position, line thickness that its p-wire, nature controlling line present are different.Therefore, when instrument test and analysis, for improving reading degree of accuracy, reach optimum Working, need to adjust respectively and optimize by different product types.The application designs and allows user according to the characteristic of test strips, pre-set corresponding one group of running parameter; Meanwhile, user can also preset many group running parameters, with the test strips of corresponding plurality of specifications.Quick switching while facilitating real work.
Technical scheme 1: a kind of CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers, is made up of image acquisition device, data processor, result follower; Motherboard handshake terminal in data processor is carried out bidirectional data exchange with image processing and the industrial camera in reading software and image acquisition device respectively.
Technical scheme 2: a kind of CCD type colloidal gold immunochromatographimethod diagnose test paper quantitative analysis method, light is sent by LED illuminating chamber, be radiated in reacted test strips, after test strips surface diffuse reflectance, light reflexes to industrial camera by optical flat mirror, industrial camera is taken generating digital image, is then sent to computer software and carries out analyzing and processing, finally obtains p-wire on colloidal gold immunochromatographimethod diagnose test paper and the reading numerical value of nature controlling line.
The present invention is compared with background technology, the one, instrument system adopts the lighting source design, self-calibration algorithm, image noise reduction algorithm of patented technology etc., effectively guarantee stability, consistance and the degree of accuracy of reading result, the subjective offset issue while having solved effectively human eye interpretation; The 2nd, instrument system the is integrated integral type application of industrial control computer framework of the application's independent intellectual property right, the parts such as the LED illumination of modular design, CCD camera, data handling system can be according to user's request, and the selection of different performance, price is provided; The 3rd, the application's software section adopts the exploitation of main flow windows platform, is convenient to follow-up function upgrading and expansion.
Accompanying drawing explanation
Fig. 1 is CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers block diagram.
Fig. 2 is the structural representation that Fig. 1 forms.
Fig. 3 is the schematic diagram of colloidal gold immunochromatographimethod diagnose test paper structure.
Fig. 4 is the execution schematic flow sheet of image processing and reading software.
Fig. 5 is the image schematic diagram in p-wire region.
Fig. 6 is the Luminance Distribution schematic diagram in p-wire region.
Fig. 7 is the block diagram of Fig. 1 implementing process.
Fig. 8 is optical module assembling schematic diagram.
Fig. 9 is colour atla schematic diagram.
Figure 10 is the structural representation of luminous source system.
Figure 11 is the schematic diagram of annular transparent base.
Figure 12 is that noise reduction is closed schematic diagram.
Figure 13 is that noise reduction is opened schematic diagram.
Figure 14 uses the reacted test strips A of same concentrations standard items, B, C.
Embodiment
Embodiment 1: with reference to accompanying drawing 1-11.A kind of CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers, is made up of image acquisition device, data processor, result follower; Motherboard handshake terminal in data processor is carried out bidirectional data exchange with image processing and the industrial camera in reading software and image acquisition device respectively.Image acquisition device is made up of target pallet, LED illuminating chamber, optical flat catoptron, industrial camera, and the face of accepting of optical flat catoptron and reflecting surface are that 45 degree distribute and the LED illuminating chamber that is positioned at optical flat catoptron below is radiated at the optical flat mirror surface that light vertical reflection to 45 degree on measured target distributes and measured target image is reflexed to industrial camera camera lens by the optical flat mirror surface distributing by 45 degree.The highly reliable 12 of low cost road white light LEDs ring illumination light source, adopt the independent adjustable white light LEDs in annular distribution 12 road, and the white diffuse reflection coating that visible light wave range reflection coefficient is greater than to 95%, realize the more uniform steady illumination to detected target.Thereby stability and the consistance of native system are improved.Software section carries out processing and the reading of image, operates under Windows system, and the flow process of its execution as shown in Figure 4.By place standard color card in CCD camera view area, software systems can be analyzed the standard color card region in the digital picture obtaining, and obtain brightness of image data; By more predefined brightness reference value and the actual brightness data obtaining, can learn the intensity deviation situation of current instrument; Software systems control electronic shutter speed, adjusts the time shutter, can change the luminance level of digital picture.According to current intensity deviation situation, suitably increase or reduce the time shutter, the intensity deviation of instrument can be limited in a less interval, thereby realize the stability of instrument readings.The three-channel brightness decay amplitude of red, green, blue (R, G, B) in colloidal gold immunochromatographimethod diagnose test paper p-wire region is different, due to G passage amplitude of variation maximum, the weight that therefore we can arrange R, G, B triple channel brightness data is: 0,1,0.Only use G passage brightness data to carry out analyzing and processing, thereby improve the detection sensitivity of this instrument system to the type test strips.The structure of colloidal gold immunochromatographimethod diagnose test paper mainly comprises sample pad, pad, analyzing film, p-wire, thing nature controlling line, adsorptive pads, and wherein, nanogold particle is combined and is fixed in pad with bioactive molecule.12 white light LEDs are placed in annular transparent base, and in pedestal, outer surface uniform fold diffuse reflection coating inner side is thinner, and skin is thicker; P-wire, the nature controlling line of detected target colloid gold immunochromatography diagnose test paper are placed in lighting source central lower; The light that white light LEDs sends, after outside diffuse reflection coating multiple reflections in transparent base, will form a more uniform circular illumination in pedestal, thereby realize the Uniform Illumination to detected target.
" CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers " have quick, easy, quantitatively, advantage accurately, can be widely used in various big hospital, health inspection body carries out result judgement to test strips different classes of or different manufacturers such as early pregnancy, influenza, drugs.Utilize the measuring accuracy of this system high, the advantage such as stable and consistent, also can use in disease surveillance mechanism, medical laboratory etc.Fig. 2 is the composition structural representation of instrument.
Light is sent by LED illuminating chamber, is radiated in reacted test strips.After test strips surface diffuse reflectance, light reflexes to industrial camera by optical flat mirror.Industrial camera is taken generating digital image, is then sent to computer software and carries out analyzing and processing.Finally obtain p-wire on colloidal gold immunochromatographimethod diagnose test paper and the reading numerical value of nature controlling line.
On test strips p-wire and nature controlling line, due to the absorption characteristic of nanogold particle to light, the scattered light signal that therefore p-wire (T line) and nature controlling line (C line) are located will be less than the signal in other region of test strips.Instant image is carried out after brightness analysis, on brightness distribution curve, should have two low value region corresponding with p-wire and nature controlling line.According to the size of these two regional luminance numerical value, can judge having or not and concentration of target checking matter in principle.
This instrument image is processed the test strips view data collecting with reading software basis, first calculates respectively the brightness value T_1 of p-wire and the brightness value C_1 of nature controlling line; Meanwhile, according to the background brightness value R_1 of scattered light on the p-wire obtaining without colloid gold particle region (background) brightness value outside p-wire; Consider the visual characteristic of human eye interpretation, we adopt the difference of background luminance value and p-wire, nature controlling line brightness value: R_1-T_1, R_1-C_1, represent p-wire in test strips and the power of nature controlling line.When being fixed on nanogold particle on p-wire when more, human eye interpretation is that p-wire is stronger, and the reading that this instrument system draws can be larger; Otherwise, when being fixed on nanogold particle on p-wire when less, human eye interpretation be p-wire a little less than, the reading that this instrument system draws also can be less.
By detecting the standard model of a series of concentration known, can draw the corresponding relation between this instrument system reading and standard model concentration.Set up the process of corresponding relation, we are referred to as calibration.The colloidal gold immunochromatographimethod diagnose test paper dissimilar or different manufacturers is produced, consistance, stability and the resolution characteristic to variable concentrations sample of its test result are different.Therefore, the calibration results is associated with the type of test strips.This instrument image is processed with reading Software for Design and is allowed user's the calibration results to be set to running parameter, during for actual read number.And allow user that many group running parameters are set simultaneously, the test strips of corresponding multiple types, choosing when facilitating real work.
" CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers " is made up of jointly hardware and software two parts.Software section carries out processing and the reading of image, operates under Windows system, and the flow process of its execution as shown in Figure 4.Consider the requirement of instrument application environment, need that a kind of illumination is stable, thermal value is low, light source that can long time continuous working.Light emitting diode, so that its volume is little, low in energy consumption, luminescence efficiency is high, the feature of controlling is convenient in brightness, becomes first-selection.On this project instrument, the application has adopted the high quality white light LED of day sub-chemistry (NICHIA).For the light that LED sends can be relatively evenly distributed in detection target, the application has adopted plurality of LEDs, annular spread to add the array mode of diffuse reflection coating; For guaranteeing LED stable luminescence, special LED feed circuit are designed.Consider performance requirement and versatility requirement that native system uses, on image is processed, the application has adopted specific aim design.
Noise reduction: because the operating characteristic of electronic equipment causes the digital picture of detected target with the noise of some.By great many of experiments, the application analyzes the rule trend that noise distributes, and has designed software noise reduction algorithm.Effectively improve the picture quality that CCD camera is taken, guaranteed the precision of reading result;
Strengthen: the light that the p-wire in test strips and nature controlling line absorb has selectivity, thereby the light reflecting under white light is no longer white light, and with color.To reading be meaningfully absorbed light number, by digital picture R, G, the each channel brightness value of B being carried out to certain increase and decrease, mask irrelevant reflection ray, can strengthen the recognition ability to detected target.
As shown in Figure 6, the three-channel brightness decay amplitude of the red, green, blue in colloidal gold immunochromatographimethod diagnose test paper p-wire region (R, G, B) is different.Due to G passage amplitude of variation maximum, the weight that therefore we can arrange R, G, B triple channel brightness data is: 0,1,0.Only use G passage brightness data to carry out analyzing and processing, thereby improve the detection sensitivity of this instrument system to the type test strips.
Below Fig. 7, from the technological process of production, critical process and safeguard measure, process modification plan aspect, process program measure is described.
Wherein, with the emphasis that is assembled into of optical module, the assemble flow of this assembly is (Fig. 8):
Innovative point one: high precision real-time luminosity, contrast, white balance self calibration.Make full use of the data operation processing power that computer system is powerful, by using standard color card, realtime graphic detection, camera brightness, contrast, white balance parameter automatic feedback control, eliminate the signal drift that this project instrument system image acquisition associated components brings because of electron device operating characteristic in the time working long hours, thereby improved the precision and stability of system.
The digital picture that the reading result of this instrument system obtains based on image acquisition component.Therefore, image acquisition associated components when work unstable, all can cause the digital picture obtaining to have certain difference, thereby affect final reading.Find through great many of experiments, affect following two broad aspect of mainly containing of instrument system measuring accuracy and stability:
(1) stability of lighting source.Because the electron devices such as LED and LED feed circuit are after working long hours, there will be the drift of running parameter.The luminescence efficiency of such as LED, feed circuit electric current, voltage etc.; In addition, the whitewash that illuminating chamber diffuse reflection coating is used, along with the increase of time, also there will be weather stain.When these factors can cause measuring, lighting condition is unstable, shows as that brightness is abnormal, colour cast etc.
(2) stability of CCD camera.Because the principle of work of CCD camera is, by optoelectronic device, light signal is converted to electric signal, in the time that instrument system works long hours, equally also can there is change to a certain degree in the working index of electron device.Finally can show brightness, contrast, R, G, B triple channel color unstable of the digital picture of output.The CCD camera price with good job stability is also higher.And the good 3-CCD camera of color rendition, its price significantly exceedes single CCD camera of general applications especially.
By place standard color card in CCD camera view area, software systems can be analyzed the standard color card region in the digital picture obtaining, and obtain brightness of image data; By more predefined brightness reference value and the actual brightness data obtaining, can learn the intensity deviation situation of current instrument; Software systems control electronic shutter speed, adjusts the time shutter, can change the luminance level of digital picture.According to current intensity deviation situation, suitably increase or reduce the time shutter, the intensity deviation of instrument can be limited in a less interval, thereby realize the stability of instrument readings.
Feature one: broken through the way that traditional test strips quantified system analysis needs regular manual maintenance.The self calibration of having realized instrument system is non-maintaining; Feature two: standard color card is included in the digital picture of system shooting.Can be used as simultaneously and judge the whether normal foundation of working state of system, for processing the reference that extremely provides strong, shown in Fig. 9.
As shown in figure 10, native system, through to 3500 continuous-readings of standard grayscale test card (the 1st grade, the 10th grade of Edmund Optics a53-712) (5 seconds, each reading interval), proves that self-calibration function can be by the vary stable of the brightness of image of CCD camera shooting, contrast, white balance in a less scope.
Innovative point two: the highly reliable 12 of low cost road white light LEDs ring illumination light source.Adopt the independent adjustable white light LEDs in annular distribution 12 road, and the white diffuse reflection coating that visible light wave range reflection coefficient is greater than to 95%, realize the more uniform steady illumination to detected target.Thereby stability and the consistance of native system are improved.The digital picture that CCD camera is taken detected target (being colloidal gold immunochromatographimethod diagnose test paper) is the basis of native system analysis and calculating.And CCD camera imaging, in principle, be detected target by light illuminating after, its reflected light enters the camera lens of camera, and is gathered and obtained by camera light electrical part.Therefore, the lighting condition of detected target will directly affect the quality of the final digital picture of exporting of CCD camera.LED (Lighting Emitting Diode) illumination is LED lighting, is a kind of semiconductor solid luminescence device.It is to utilize solid semiconductor chip as luminescent material, in semiconductor, occurs compoundly to emit superfluous energy and cause photo emissions by charge carrier, directly sends red, yellow, blue, green, blue or green, orange, purple, white light.The feature such as energy-saving and environmental protection, life-span that LED has are long, volume is little, makes it become the preferred lighting source solution of native system.But, in actual quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers, use LED illumination, also need to solve homogeneity, the stability problem of illumination.
As shown in figure 11,12 white light LEDs are placed in annular transparent base, outer surface uniform fold diffuse reflection coating (inner side is thinner, and skin is thicker) in pedestal.P-wire, the nature controlling line of detected target (colloidal gold immunochromatographimethod diagnose test paper) are placed in lighting source central lower.The light that white light LEDs sends, after outside diffuse reflection coating multiple reflections in transparent base, will form a more uniform circular illumination in pedestal, thereby realize the Uniform Illumination to detected target.
LED is typical current mode element, and in the time opening on operating voltage, all acute variation with incoming current likely of the subtle change of voltage, cause the acute variation of power input and output intensity.For the current mode element of this class, from simple technical standpoint, preferably can adopt constant current source power supply.But constant current source power supply circuit structure complexity, cost costliness, application is anti-rather than a lot.By the resistance of choose reasonable current-limiting resistance, adopting constant pressure source can be also LED power supply.This type of drive is simple in structure, uses flexibly.
This project adopts multichannel independence voltage stabilizing design, and can realize millivolt level output fine setting.When instrument system is after use after a while, can, by adjusting the output voltage of feed circuit, proofread and correct the variation of LED working index.
Innovative point three: the abnormal numerical value filter algorithm of analyzing based on dispersion
Native system is based on digital image processing techniques.When software systems work, using pixel as base unit.The p-wire of colloidal gold immunochromatographimethod diagnose test paper, nature controlling line, on the image obtaining, are rendered as a specific region.Because this region comprises multiple pixels conventionally, therefore, the brightness value in region exists the sampling method problem to multiple pixel brightness values.Conventional way is to average.That is: in regional luminance value=region each pixel brightness value and/pixel number.But in real work, colloidal gold immunochromatographimethod diagnose test paper, because of the reason of manufacturing process, exists nanogold particle problem pockety on p-wire, nature controlling line; Meanwhile, because test sample book (urine, blood, saliva etc.) is conventionally with impurity, can in test strips, form stain; In addition, due to operating personnel and operating environment, once in a while also can be to bringing impurity in test strips, as floating dust, hair etc.Above-mentioned impact, is embodied on the brightness value in p-wire, nature controlling line region, can show as abnormal (higher or on the low side) of multiple pixel brightness values.
The deviation of reading causing for weakening this type of factor, this project Software for Design based on dispersion analyze abnormal numerical value filter algorithm.When the brightness value of some pixel in p-wire, nature controlling line region is during below or above limited range (being determined by the predefined dispersion control of user parameter), the data of this location of pixels will be excluded in calculating, not as sampling spot.For example, common broken string region, its brightness value can be obviously higher; Collaurum build-up areas, its brightness value can be obviously on the low side.
For example, as shown in figure 14.Use the reacted test strips A of same concentrations standard items, B, C.Closing and opening the reading result of algorithm.
| Test strips A | Test strips B | Test strips C | Mean value | |
| Close the reading of algorithm | 65.94 | 66.02 | 66.59 | 66.18 |
| Open the reading of algorithm | 73.61 | 71.12 | 73.77 | 72.83 |
Can find out from reading numerical value, open algorithm and can effectively reduce broken string region and drag down the impact of reading.
Through lot of experiment validation, this algorithm has the following advantages with respect to conventional area Sampling Method
Advantage one: the reliability can improve system works time, reduce the frequency of occurrences of abnormal reading result, promote effectively the practical value of native system;
Advantage two: the tolerance that has effectively strengthened the test strips of the different qualities of production that native system provides different vendor.During to nanogold particle test strips product pockety reading, system results also can keep highly consistent with human eye sentence read result.
Embodiment 2: on the basis of embodiment 1, the quantitative analysis method of a kind of CCD type colloidal gold immunochromatographimethod diagnose test paper, it is characterized in that: light is sent by LED illuminating chamber, be radiated in reacted test strips, after test strips surface diffuse reflectance, light reflexes to industrial camera by optical flat mirror, and industrial camera is taken generating digital image, then be sent to computer software and carry out analyzing and processing, finally obtain p-wire on colloidal gold immunochromatographimethod diagnose test paper and the reading numerical value of nature controlling line.On test strips p-wire and nature controlling line, due to the absorption characteristic of nanogold particle to light, the scattered light signal at therefore p-wire--T line and nature controlling line--C line place will be less than the signal in other region of test strips, instant image is carried out after brightness analysis, on brightness distribution curve, should there are two low value region corresponding with p-wire and nature controlling line, in principle according to the size of these two regional luminance numerical value, can judge having or not and concentration of target checking matter, that is: in the time detecting, first the sample drop that contains target checking matter is added in sample pad, sample enters pad by infiltration and syphonic effect, the analyzing film of flowing through, flow to adsorptive pads, in this process, on analyzing film p-wire and nature controlling line, complicated immune response will be there is, after immunochromatography reaction finishes, on p-wire and nature controlling line, be all fixed with nanogold particle, and on p-wire in the content of nanogold particle and sample the concentration of target checking matter have corresponding relation, if not containing target checking matter, just there is no combining nano gold grain on p-wire in sample.
It is to be understood that: it is to be understood that: although above-described embodiment is to the mentality of designing of the present invention detailed text description of contrasting; but these text descriptions; the just simple description to mentality of designing of the present invention; rather than restriction to mentality of designing of the present invention; any combination, increase or modification that does not exceed mentality of designing of the present invention, all falls within the scope of protection of the present invention.
Claims (4)
1. a CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers, is characterized in that being made up of image acquisition device, data processor, result follower; Motherboard handshake terminal in data processor is carried out bidirectional data exchange with image processing and the industrial camera in reading software and image acquisition device respectively; Image acquisition device is made up of target pallet, LED illuminating chamber, optical flat catoptron, industrial camera, and the face of accepting of optical flat catoptron and reflecting surface are that 45 degree distribute and the LED illuminating chamber that is positioned at optical flat catoptron below is radiated at the optical flat mirror surface that light vertical reflection to 45 degree on measured target distributes and measured target image is reflexed to industrial camera camera lens by the optical flat mirror surface distributing by 45 degree; 12 road white light LEDs ring illumination light sources, adopt the independent adjustable white light LEDs in annular distribution 12 road, and the white diffuse reflection coating that visible light wave range reflection coefficient is greater than to 95%, realize the more uniform steady illumination to detected target, thereby improved stability and the consistance of native system; By place standard color card in CCD camera view area, software systems can be analyzed the standard color card region in the digital picture obtaining, and obtain brightness of image data; By more predefined brightness reference value and the actual brightness data obtaining, can learn the intensity deviation situation of current instrument; Software systems control electronic shutter speed, adjust the time shutter, can change the luminance level of digital picture, according to current intensity deviation situation, suitably increase or reduce the time shutter, the intensity deviation of instrument can be limited in a less interval, thereby realize the stability of instrument readings.
2. CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers according to claim 1, is characterized in that: software section carries out processing and the reading of image, and operate under Windows system, the flow process of its execution is as follows:
3. CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers according to claim 1, it is characterized in that: the three-channel brightness decay amplitude of red, green, blue (R, G, B) in colloidal gold immunochromatographimethod diagnose test paper p-wire region is different, due to G passage amplitude of variation maximum, therefore the weight that we can arrange R, G, B triple channel brightness data is: 0,1,0, only use G passage brightness data to carry out analyzing and processing, thereby improve the detection sensitivity of this instrument system to the type test strips.
4. CCD type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers according to claim 3, it is characterized in that: the structure of colloidal gold immunochromatographimethod diagnose test paper mainly comprises sample pad, pad, analyzing film, p-wire, nature controlling line, adsorptive pads, wherein, nanogold particle is combined and is fixed in pad with bioactive molecule.
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