CN109324046A - Test strips reaction density detection method, device, storage medium and computer equipment - Google Patents
Test strips reaction density detection method, device, storage medium and computer equipment Download PDFInfo
- Publication number
- CN109324046A CN109324046A CN201710641708.9A CN201710641708A CN109324046A CN 109324046 A CN109324046 A CN 109324046A CN 201710641708 A CN201710641708 A CN 201710641708A CN 109324046 A CN109324046 A CN 109324046A
- Authority
- CN
- China
- Prior art keywords
- test strips
- reaction density
- light metering
- brightness value
- metering parameter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 253
- 238000001514 detection method Methods 0.000 title claims abstract description 59
- 238000002835 absorbance Methods 0.000 claims abstract description 52
- 238000000034 method Methods 0.000 claims abstract description 14
- 238000002310 reflectometry Methods 0.000 claims description 14
- 230000004044 response Effects 0.000 claims description 10
- 238000004590 computer program Methods 0.000 claims description 6
- 230000008569 process Effects 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 9
- 238000010586 diagram Methods 0.000 description 6
- 238000007689 inspection Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 4
- 230000008033 biological extinction Effects 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- FRPHFZCDPYBUAU-UHFFFAOYSA-N Bromocresolgreen Chemical compound CC1=C(Br)C(O)=C(Br)C=C1C1(C=2C(=C(Br)C(O)=C(Br)C=2)C)C2=CC=CC=C2S(=O)(=O)O1 FRPHFZCDPYBUAU-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000012812 general test Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- PRZSXZWFJHEZBJ-UHFFFAOYSA-N thymol blue Chemical compound C1=C(O)C(C(C)C)=CC(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C(=CC(O)=C(C(C)C)C=2)C)=C1C PRZSXZWFJHEZBJ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention provides a kind of test strips reaction density detection method, device, storage medium and its computer equipment, its specifically: acquisition test strips react image, the color in image is reacted according to test strips, identify hue regions in the test strips reaction image, obtain the corresponding light metering parameter of the hue regions, the light metering parameter includes brightness value or absorbance, according to the light metering parameter and default light metering parameter and test strips reaction density corresponding relationship, test strip reaction density.In whole process, it is based on brightness value or absorbance, test strips reaction density is obtained using rigorous data acquisition and procession mode, avoids the interference of human factor, it being capable of accurate test strip reaction density.
Description
Technical field
The present invention relates to detection technique field is examined, more particularly to test strips reaction density detection method, device, storage
Medium and computer equipment.
Background technique
Dry analysis technology is referred to and fluid test sample is applied directly to as not for wet-chemical technique
It is specific chemical anti-with being caused on the commercialized dried reagent item of the specific production of project using the moisture of sample as solvent
It answers, so that the method for carrying out chemical analysis, is a kind of analysis method based on enzyme process, and has dry reagent chemistry or immobilization
Title.
Currently, mainly being detected to some test strips based on the dry chemical detection in dry analysis technical foundation
And it is analyzed.Such as detecting for test strips reaction density is mainly that qualitative detection test strips reaction density is negative or is sun
Property, still can be with test paper strip for semi-quantitative detecting reaction density-,-/+,+, ++, +++ or ++++.
The detection of test strips reaction density for a long time relies primarily on range estimation, i.e., with the colour developing or inspection on human eye observation's test paper
The shade of survey line can only accomplish simple qualitatively judgement, and human factor is affected to the judgement of testing result, encounters
Measurand content is very low, when developing the color very shallow, often relies on naked eyes and determines that result is difficult, it is easy to cause to judge by accident, generate
The test strips reaction density testing result of mistake.
Summary of the invention
Based on this, it is necessary to for the testing result inaccuracy problem of general test strips reaction density detection method, provide
A kind of accurate test strips reaction density detection method of testing result, device, storage medium and its computer equipment.
A kind of test strips reaction density detection method, comprising steps of
It acquires test strips and reacts image；
The color in image is reacted according to test strips, identifies hue regions in the test strips reaction image；
The corresponding light metering parameter of the hue regions is obtained, the light metering parameter includes brightness value or absorbance；
According to the light metering parameter and default light metering parameter and test strips reaction density corresponding relationship, Test paper
Reaction density.
A kind of test strips reaction density detection device, comprising:
Image capture module, for acquiring test strips reaction image；
Identification module identifies tone in the test strips reaction image for reacting the color in image according to test strips
Region；
Parameter acquisition module, for obtaining the corresponding light metering parameter of the hue regions, the light metering parameter includes
Brightness value or absorbance；
Detection module, for corresponding with test strips reaction density according to the light metering parameter and default light metering parameter
Relationship, test strip reaction density.
Test strips reaction density detection method of the present invention and device, acquisition test strips are reacted image, are reacted according to test strips
Color in image identifies hue regions in the test strips reaction image, obtains the corresponding light metering ginseng of the hue regions
Number, the light metering parameter includes brightness value or absorbance, according to the light metering parameter and default light metering parameter and examination
Paper slip reaction density corresponding relationship, test strip reaction density.In whole process, it is based on brightness value or absorbance, using tight
Careful data acquisition and procession mode obtains test strips reaction density, avoids the interference of human factor, being capable of accurate Test paper
Reaction density.
In addition, being stored thereon with computer program the present invention also provides a kind of computer readable storage medium, which is located
It manages when device executes and realizes such as the step of the above method.
In addition, the present invention also provides a kind of computer equipment, including memory, processor and storage are on a memory and can
The computer program run on a processor, processor are realized when executing program such as the step of the above method.
Above-mentioned computer readable storage medium and computer equipment, wherein test strips reaction density detection method, is based on bright
Angle value or absorbance obtain test strips reaction density using rigorous data acquisition and procession mode, avoid the dry of human factor
It disturbs, it being capable of accurate test strip reaction density.
Detailed description of the invention
Fig. 1 is the flow diagram of test strips reaction density detection method one embodiment of the present invention；
Fig. 2 is that sample to be tested is dropped in test strips, and each area schematic after color reaction occurs for test strips；
Fig. 3 is the flow diagram of second embodiment of test strips reaction density detection method of the present invention；
Fig. 4 is the flow diagram of the one of specific application example of test strips reaction density detection method of the present invention；
Fig. 5 is the flow diagram of the one of specific application example of test strips reaction density detection method of the present invention
Fig. 6 is the structural schematic diagram of test strips reaction density detection device one embodiment of the present invention；
Fig. 7 is the structural schematic diagram of second embodiment of test strips reaction density detection device of the present invention.
Specific embodiment
As shown in Figure 1, a kind of test strips reaction density detection method, comprising steps of
S200: acquisition test strips react image.
Sample to be tested drips in test strips, and color reaction, such as common PH test paper can generally occur for test strips.PH test paper
On have three kinds of methyl red, bromocresol green and thymol blue indicator, they in the solution of different PH can by one set pattern
Rule discoloration.PH test paper is very widely used, when the pH value of test solution, dips prepare liquid point with the glass bar of clean dried
It drips the color in the middle part of test paper, after observation variation is stablized and judges the property of solution with standard color comparison card comparison.Specifically,
Test strips may include dry chemical scrip, colloidal gold immuno-chromatography test paper strip and test card etc..
Herein, image capture device can be used directly to shoot, acquire the image data after test strips color reaction,
Such as digital camera, CCD (Charge-coupled Device, charge coupled cell) camera and intelligent hand can be passed through
The equipment such as machine directly shoot the image after test strips color reaction.
S400: reacting the color in image according to test strips, identifies hue regions in the test strips reaction image.
There is the color different from test strips true qualities, needle after test strips color reaction in the image of step S200 acquisition
(image procossing) is further processed to the image of shooting, so that it may collect the tone zone after test strips color reaction in test strips
Domain.Specifically, hue regions refer to the region that color reaction occurs in test strips, can characterize test strips reaction density.
Continuing by taking PH test paper as an example, test strips itself are generally yellow, and when drippage has alkaline solution, color reaction occurs for PH test paper,
Become blue；When drippage has acid solution to be, color reaction occurs for PH test paper, becomes red.Since the sample to be tested of drippage is molten
Liquid is limited, and PH test paper will not all drip sample to be tested solution, i.e., true qualities yellow area and test strips can be presented on PH test paper
Blue region (alkaline sample to be tested solution) after reaction；Or the red area after true qualities yellow area and test strips reaction is presented
(acid sample to be tested solution).Further, as shown in Fig. 2, in Fig. 2, in entire test paper strip area A, B area occurs
The region of test strips reaction, the color presented are the color after test strips reaction, and corresponding image is hue regions；
The region C is the region (not dripped sample to be tested) that test strips reaction does not occur, and the color presented is test strips intrinsic colour.
S600: obtaining the corresponding light metering parameter of the hue regions, and the light metering parameter includes brightness value or extinction
Degree.
Brightness refers to the physical quantity of (reflective) power of illuminator (refractive body) surface light emitting.Human eye light from a direction
Singly source, the light source area ratio of light intensity and human eye institute " seeing " in this direction, is defined as the brightness of the light source unit, i.e.,
Luminous intensity in the projected area of position.Absorbance refers to that light passes through the incident intensity and the light before solution or a certain substance
Pass through the denary logarithm of the transmitted intensity ratio after solution or substance.Specifically, color is got in step S400
After adjusting region, image processing software can be used, obtains the corresponding light metering parameter of hue regions, such as can pass through
Photoshop image processing software obtains the corresponding light metering parameter of hue regions, and light metering parameter can specifically include bright
Angle value or absorbance.
S800: according to the light metering parameter and default light metering parameter and test strips reaction density corresponding relationship, inspection
Test paper slip reaction density.
Default light metering parameter and test strips reaction density corresponding relationship are preset corresponding relationships, specifically can be with
It is obtained based on the analyses such as historical empirical data and test data.Specifically, it presets light metering parameter and reacts dense with test strips
Spending corresponding relationship may include brightness value-test strips reaction density corresponding relationship, and/or, absorbance-test strips reaction density pair
It should be related to.It can specifically be selected according to the parameter that light metering parameter includes in step S600, i.e., when light metering in step S600
When parameter includes brightness value, step S800 selection is according to brightness value and brightness value-test strips reaction density corresponding relationship, detection
Test strips reaction density；When light metering parameter includes absorbance in step S600, step S800 selection according to absorbance and
Absorbance-test strips reaction density corresponding relationship, test strip reaction density.
Specifically, test strip reaction density may include two levels, and one is to carry out qualitative detection, that is, detect
Test strips reaction density is characterized as negative or is positive；The other is half-quantitative detection, i.e. test strip reaction density are carried out,
Test strips reaction density can be usually characterized using the quantity of "+" and is characterized as positive degree, such as can be "+", " ++ "
And " +++ " etc..It is closed herein according to the light metering parameter and default light metering parameter are corresponding with test strips reaction density
System, test strip reaction density, furthermore it is also possible to which further half-quantitative detection is characterized as positive degree.It is obtaining
After testing result, result output can be will test.
Test strips reaction density detection method of the present invention, acquisition test strips are reacted image, are reacted in image according to test strips
Color, identify hue regions in test strips reaction image, obtain the corresponding light metering parameter of the hue regions, it is described
Light metering parameter includes brightness value or absorbance, is reacted according to the light metering parameter and default light metering parameter with test strips
Concentration corresponding relationship, test strip reaction density.In whole process, it is based on brightness value or absorbance, using rigorous data
Acquisition obtains test strips reaction density with processing mode, avoids the interference of human factor, accurately test strip can react dense
Degree.
As shown in figure 3, the light metering parameter includes absorbance in one of the embodiments, step S600 includes:
S620: the corresponding brightness value of the hue regions is obtained；
S640: according to the brightness value, the corresponding reflectivity of the hue regions is obtained；
S660: by the reflectivity, the corresponding absorbance of the hue regions is obtained.
The corresponding absorbance of hue regions can be directly acquired using image software, after can also being converted using brightness value
It obtains.In the present embodiment, we convert to obtain corresponding absorbance by brightness value.In the present embodiment, pass through first
Image processing software directly acquires the corresponding brightness value of hue regions, for example, can by Photoshop image processing software come
The corresponding brightness value of hue regions is obtained, the corresponding reflectivity in carving region is penetrated further according to brightness value calculating, due to reflectivity, extinction
Degree and light transmittance and be equal to 1, for test strips its light transmittance can approximation to regard as be 0, i.e. absorbance=1- reflection
Rate.The process of above-mentioned acquisition absorbance can be obtained using being calculated according to parameter and specific formula for calculation, can also pass through processing
Software is obtained using lookup table mode.
The light metering parameter includes brightness value in one of the embodiments, it is described according to the light metering parameter with
And default light metering parameter and test strips reaction density corresponding relationship, the step of test strip reaction density, include:
According to the brightness value and predetermined luminance value and test strips reaction density corresponding relationship, test strip reaction is dense
Degree.
It is further, described according to the brightness value and predetermined luminance value and test strips reaction density corresponding relationship,
Before the step of test strip reaction density further include:
Step 1: the corresponding brightness value of test strips differential responses concentration is obtained；
Step 2: according to the corresponding brightness value of the test strips differential responses concentration, it is dense to construct brightness value-test strips reaction
Spend relation curve；
Step 3: it according to the brightness value-test strips reaction density relation curve, obtains predetermined luminance value and test strips is anti-
Answer concentration corresponding relationship.
In practical applications, quasi- inspection ingredient can be configured to standard from high to low (or from low to high) according to concentration
Gradient liquid, the normal gradients liquid correspond to different test strips reaction densities, such as can be corresponding in turn to-,-/+,+, ++, +++.It should
Normal gradients liquid is dropped in respectively in different test strips, acquires the brightness value after each test strips color reaction.It is non-essential, needle
The sample to be tested of same concentrations can be dropped in respectively in multiple identical test strips, obtains corresponding brightness respectively
Value, final averaged carry out the more acurrate brightness value obtained under test strips differential responses concentration.The test strips reaction that will acquire
Concentration and brightness value are plotted in chart, construct brightness value-concentration relationship curve, brightness value-concentration relationship based on the building
Curve can obtain brightness value and test strips reaction density corresponding relationship using multinomial fit approach.
Light metering parameter includes absorbance in one of the embodiments, described according to the light metering parameter and pre-
If the step of light metering parameter and test strips reaction density corresponding relationship, test strip reaction density, includes:
According to the absorbance and default absorbance and test strips reaction density corresponding relationship, test strip reaction is dense
Degree.
It is further, described according to the absorbance and default absorbance and test strips reaction density corresponding relationship,
Before the step of test strip reaction density further include:
Step 1: the corresponding absorbance of test strips differential responses concentration is obtained；
Step 2: according to the corresponding absorbance of the test strips differential responses concentration, it is dense to construct absorbance-test strips reaction
Spend relation curve；
Step 3: it according to the absorbance-test strips reaction density relation curve, obtains default absorbance and test strips is anti-
Answer concentration corresponding relationship.
In practical applications, quasi- inspection ingredient can be configured to standard from high to low (or from low to high) according to concentration
Gradient liquid, the normal gradients liquid correspond to different test strips reaction densities, such as can be corresponding in turn to-,-/+,+, ++, +++.It should
Normal gradients liquid is dropped in respectively in different test strips, acquires the absorbance after each test strips color reaction.It is non-essential, needle
The sample to be tested of same concentrations can be dropped in respectively in multiple identical test strips, obtains corresponding extinction respectively
Degree, final averaged carry out the more acurrate absorbance obtained under test strips differential responses concentration.The test strips reaction that will acquire
Concentration and absorbance are plotted in chart, construct absorbance-concentration relationship curve, absorbance-concentration relationship based on the building
Curve can obtain absorbance and test strips reaction density corresponding relationship using multinomial fit approach.It is non-essential, corresponding relationship
It can be function corresponding relationship, can also directly be characterized as the corresponding relationship of curve chart.
For the technical solution for further explaining in detail test strips reaction density detection method of the present invention, use separately below
Two embodiments, and be illustrated in conjunction with attached drawing 4 and Fig. 5.
Embodiment one: light metering parameter includes brightness value, as shown in figure 4, test strips reaction density detection method of the present invention
The following steps are included:
1.1, quasi- inspection ingredient is configured to normal gradients liquid according to concentration from high to low, the corresponding difference of normal gradients liquid
Test strips reaction density, such as can be corresponding in turn to-,-/+,+, ++, +++；
1.2, normal gradients drop is fallen in each test strips, color reaction occurs in each test strips, acquisition test strips are anti-
Answer image；
1.3, the color in image is reacted according to test strips, identifies hue regions in the test strips reaction image；
1.4, the brightness value of hue regions is obtained；
1.5, brightness value-concentration relationship curve is drawn, brightness value and test strips reaction density corresponding relationship are obtained and is stored；
1.6, when needing to carry out the detection of test strips reaction density, the figure after test strips color reaction is shot by CCD
Picture；
1.7, the color in image is reacted according to test strips, identifies hue regions in the test strips reaction image.
1.8, the brightness value of hue regions is obtained；
1.9, qualitative to sentence according to the brightness value and test strips reaction density corresponding relationship stored in brightness value and step 1.5
Disconnected test strips reaction density is positive (+) or is negative (-)；Further, according to the brightness value and test paper stored in step 1.5
Reaction density corresponding relationship, test paper strip for semi-quantitative detecting reaction density is-/+,+, ++ or +++.
Embodiment two: light metering parameter includes absorbance, as shown in figure 5, test strips reaction density detection method of the present invention
The following steps are included:
2.1, quasi- inspection ingredient is configured to normal gradients liquid according to concentration from high to low, the corresponding difference of normal gradients liquid
Test strips reaction density, such as can be corresponding in turn to-,-/+,+, ++, +++；
2.2, normal gradients drop is fallen in each test strips, color reaction occurs in each test strips, acquisition test strips are anti-
Answer image；
2.3, the color in image is reacted according to test strips, identifies hue regions in the test strips reaction image；
2.4, the absorbance of hue regions is obtained；
2.5, absorbance-concentration relationship curve is drawn, absorbance and test strips reaction density corresponding relationship are obtained and is stored；
2.6, when needing to carry out the detection of test strips reaction density, the figure after test strips color reaction is shot by CCD
Picture；
2.7, the color in image is reacted according to test strips, identifies hue regions in the test strips reaction image.
2.8, the brightness value of hue regions is obtained；
2.9, according to brightness value, reflectivity is calculated；
3.0, by reflectivity, the absorbance of hue regions is calculated；
3.1, qualitative to sentence according to the absorbance and test strips reaction density corresponding relationship stored in absorbance and step 2.5
Disconnected test strips reaction density is positive (+) or is negative (-)；
3.2 is further, according to the absorbance and test strips reaction density corresponding relationship stored in step 2.5, sxemiquantitative
Test strip reaction density be-/+,+, ++ or +++.
As shown in fig. 6, a kind of test strips reaction density detection device, comprising:
Image capture module 200, for acquiring test strips reaction image；
Identification module 400 identifies color in the test strips reaction image for reacting the color in image according to test strips
Adjust region；
Parameter acquisition module 600, for obtaining the corresponding light metering parameter of the hue regions, the light metering parameter packet
Include brightness value or absorbance；
Detection module 800, for according to the light metering parameter and default light metering parameter and test strips reaction density
Corresponding relationship, test strip reaction density.
Test strips reaction density detection device of the present invention, image capture module 200 acquires test strips and reacts image, according to examination
Paper slip reacts the color in image, and identification module 400 identifies hue regions in the test strips reaction image, parameter acquisition module
600 obtain the corresponding light metering parameter of the hue regions, and the light metering parameter includes brightness value or absorbance, detection module
800 according to the light metering parameter and default light metering parameter and test strips reaction density corresponding relationship, and test strip is anti-
Answer concentration.In whole process, it is based on brightness value or absorbance, it is anti-that test strips are obtained using rigorous data acquisition and procession mode
Concentration is answered, the interference of human factor is avoided, it being capable of accurate test strip reaction density.
As shown in fig. 7, the light metering parameter includes absorbance in one of the embodiments, the parameter obtains mould
Block 600 includes:
Brightness value acquiring unit 620, for obtaining the corresponding brightness value of the hue regions；
Reflectivity computing unit 640, for obtaining the corresponding reflectivity of the hue regions according to the brightness value；
Absorbance computing unit 660, for obtaining the corresponding absorbance of the hue regions by the reflectivity.
The detection module 800 includes: in one of the embodiments,
First detection unit, for according to the light metering parameter and default light metering parameter and test strips reaction density
Corresponding relationship, qualitative detection test strips reaction density are negative or are positive；
Or,
Second detection unit, for according to the light metering parameter and default light metering parameter and test strips reaction density
Corresponding relationship, test paper strip for semi-quantitative detecting reaction density, the test strips reaction density include-,-/+,+, ++ and +++.
The light metering parameter includes brightness value in one of the embodiments, and the detection module 800 is also used to basis
The brightness value and predetermined luminance value and test strips reaction density corresponding relationship, test strip reaction density.
Further, test strips reaction density detection device of the present invention further include:
Preset value obtains module, for obtaining the corresponding brightness value of test strips differential responses concentration, according to the test strips
The corresponding brightness value of differential responses concentration constructs brightness value-test strips reaction density relation curve, according to the brightness value-examination
Paper slip reaction density relation curve obtains predetermined luminance value and test strips reaction density corresponding relationship.
In addition, being stored thereon with computer program the present invention also provides a kind of computer readable storage medium, which is located
It manages when device executes and realizes such as the step of the above method.
In addition, the present invention also provides a kind of computer equipment, including memory, processor and storage are on a memory and can
The computer program run on a processor, processor are realized when executing program such as the step of the above method.
Above-mentioned computer readable storage medium and computer equipment, wherein test strips reaction density detection method, is based on bright
Angle value or absorbance obtain test strips reaction density using rigorous data acquisition and procession mode, avoid the dry of human factor
It disturbs, it being capable of accurate test strip reaction density.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of test strips reaction density detection method, which is characterized in that comprising steps of
It acquires test strips and reacts image；
The color in image is reacted according to test strips, identifies hue regions in the test strips reaction image；
The corresponding light metering parameter of the hue regions is obtained, the light metering parameter includes brightness value or absorbance；
According to the light metering parameter and default light metering parameter and test strips reaction density corresponding relationship, test strip is anti-
Answer concentration.
2. test strips reaction density detection method according to claim 1, which is characterized in that the light metering parameter includes
Absorbance, described the step of obtaining the hue regions corresponding light metering parameter include:
Obtain the corresponding brightness value of the hue regions；
According to the brightness value, the corresponding reflectivity of the hue regions is obtained；
By the reflectivity, the corresponding absorbance of the hue regions is obtained.
3. test strips reaction density detection method according to claim 1, which is characterized in that the light metering parameter includes
Brightness value, it is described according to the light metering parameter and default light metering parameter and test strips reaction density corresponding relationship, detection
The step of test strips reaction density includes:
According to the brightness value and predetermined luminance value and test strips reaction density corresponding relationship, test strip reaction density.
4. test strips reaction density detection method according to claim 3, which is characterized in that described according to the brightness value
And predetermined luminance value and test strips reaction density corresponding relationship, the step of test strip reaction density before further include:
Obtain the corresponding brightness value of test strips differential responses concentration；
According to the corresponding brightness value of the test strips differential responses concentration, brightness value-test strips reaction density relation curve is constructed；
According to the brightness value-test strips reaction density relation curve, it is corresponding with test strips reaction density to obtain predetermined luminance value
Relationship.
5. test strips reaction density detection method according to claim 1, which is characterized in that described according to the light metering
The step of parameter and default light metering parameter and test strips reaction density corresponding relationship, test strip reaction density includes:
According to the light metering parameter and default light metering parameter and test strips reaction density corresponding relationship, qualitative detection test paper
Reaction density is negative or is positive；
Or,
According to the light metering parameter and default light metering parameter and test strips reaction density corresponding relationship, half-quantitative detection examination
Paper slip reaction density, the test strips reaction density include-,-/+,+, ++ and +++.
6. a kind of test strips reaction density detection device characterized by comprising
Image capture module, for acquiring test strips reaction image；
Identification module identifies hue regions in the test strips reaction image for reacting the color in image according to test strips；
Parameter acquisition module, for obtaining the corresponding light metering parameter of the hue regions, the light metering parameter includes brightness
Value or absorbance；
Detection module, for being closed according to the light metering parameter and default light metering parameter are corresponding with test strips reaction density
System, test strip reaction density.
7. test strips reaction density detection device according to claim 6, which is characterized in that the light metering parameter includes
Absorbance, the parameter acquisition module include:
Brightness value acquiring unit, for obtaining the corresponding brightness value of the hue regions；
Reflectivity computing unit, for obtaining the corresponding reflectivity of the hue regions according to the brightness value；
Absorbance computing unit, for obtaining the corresponding absorbance of the hue regions by the reflectivity.
8. test strips reaction density detection device according to claim 6, which is characterized in that the detection module includes:
First detection unit, for corresponding with test strips reaction density according to the light metering parameter and default light metering parameter
Relationship, qualitative detection test strips reaction density are negative or are positive；
Or,
Second detection unit, for corresponding with test strips reaction density according to the light metering parameter and default light metering parameter
Relationship, test paper strip for semi-quantitative detecting reaction density, the test strips reaction density include-,-/+,+, ++ and +++.
9. a kind of computer readable storage medium, is stored thereon with computer program, which is characterized in that the program is held by processor
It is realized when row such as the step of any one of claim 1-5 the method.
10. a kind of computer equipment, can run on a memory and on a processor including memory, processor and storage
Computer program, which is characterized in that the processor is realized as described in any one of claim 1-5 when executing described program
The step of method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710641708.9A CN109324046A (en) | 2017-07-31 | 2017-07-31 | Test strips reaction density detection method, device, storage medium and computer equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710641708.9A CN109324046A (en) | 2017-07-31 | 2017-07-31 | Test strips reaction density detection method, device, storage medium and computer equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109324046A true CN109324046A (en) | 2019-02-12 |
Family
ID=65245024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710641708.9A Pending CN109324046A (en) | 2017-07-31 | 2017-07-31 | Test strips reaction density detection method, device, storage medium and computer equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109324046A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040022678A1 (en) * | 2000-03-17 | 2004-02-05 | Yoshiyuki Komagoe | Test paper |
| US20060069552A1 (en) * | 2004-08-20 | 2006-03-30 | Spitler Mark T | Apparatus and method for displaying signals of a detector |
| JP2007285988A (en) * | 2006-04-20 | 2007-11-01 | Wako Pure Chem Ind Ltd | Analyzing device and measuring method |
| CN101387601A (en) * | 2007-09-15 | 2009-03-18 | 周中人 | PC type immune test paper image recognition and quantitative analyzer |
| CN102279263A (en) * | 2011-05-06 | 2011-12-14 | 杭州顿力医疗器械股份有限公司 | CCD-type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers |
| CN102879562A (en) * | 2012-09-27 | 2013-01-16 | 上海奥普生物医药有限公司 | Colloidal gold immunofiltration quantitive detection method and reagent kit |
| CN103267843A (en) * | 2013-06-08 | 2013-08-28 | 张沈平 | Colloidal gold test paper as well as corresponding colloidal gold analyzer and testing method |
| CN103335955A (en) * | 2013-06-19 | 2013-10-02 | 华南农业大学 | Water quality on-line monitoring method and device |
| CN103984964A (en) * | 2014-06-05 | 2014-08-13 | 爱威科技股份有限公司 | Image identification method and system for test strip |
| CN107066779A (en) * | 2016-10-18 | 2017-08-18 | 应继伟 | Self-service body parameter inspection method and system based on biological specimen color |
-
2017
- 2017-07-31 CN CN201710641708.9A patent/CN109324046A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040022678A1 (en) * | 2000-03-17 | 2004-02-05 | Yoshiyuki Komagoe | Test paper |
| US20060069552A1 (en) * | 2004-08-20 | 2006-03-30 | Spitler Mark T | Apparatus and method for displaying signals of a detector |
| JP2007285988A (en) * | 2006-04-20 | 2007-11-01 | Wako Pure Chem Ind Ltd | Analyzing device and measuring method |
| CN101387601A (en) * | 2007-09-15 | 2009-03-18 | 周中人 | PC type immune test paper image recognition and quantitative analyzer |
| CN102279263A (en) * | 2011-05-06 | 2011-12-14 | 杭州顿力医疗器械股份有限公司 | CCD-type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers |
| CN102879562A (en) * | 2012-09-27 | 2013-01-16 | 上海奥普生物医药有限公司 | Colloidal gold immunofiltration quantitive detection method and reagent kit |
| CN103267843A (en) * | 2013-06-08 | 2013-08-28 | 张沈平 | Colloidal gold test paper as well as corresponding colloidal gold analyzer and testing method |
| CN103335955A (en) * | 2013-06-19 | 2013-10-02 | 华南农业大学 | Water quality on-line monitoring method and device |
| CN103984964A (en) * | 2014-06-05 | 2014-08-13 | 爱威科技股份有限公司 | Image identification method and system for test strip |
| CN107066779A (en) * | 2016-10-18 | 2017-08-18 | 应继伟 | Self-service body parameter inspection method and system based on biological specimen color |
Non-Patent Citations (2)
| Title |
|---|
| MARIA JOSÉ GONZÁLEZ-GUERRERO ET AL.: "Paper-based microfluidic biofuel cell operating under glucose concentrations within physiological range", 《BIOSENSORS AND BIOELECTRONICS》 * |
| 朱健铭等: "特征光谱吸收法测量人血清前列腺特异性抗原浓度的研究", 《光谱学与光谱分析》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108051434B (en) | Color recognition-based quantitative detection method for concentration of liquid to be detected | |
| TWI795453B (en) | Methods and devices for performing an analytical measurement based on a color formation reaction | |
| CN109324039A (en) | Test strips reaction density detection method, device, storage medium and computer equipment | |
| CN106546581A (en) | Detection paper card intelligent checking system and detection paper card intelligent analysis method | |
| CN107884396B (en) | Optical microscope-based ascorbic acid concentration online sensing analysis method and auxiliary color developing agent | |
| KR101624583B1 (en) | Urine Examination Method and Related Devices | |
| CN112074725A (en) | Detection test paper reader system based on accurate colorimetry | |
| WO2015038717A1 (en) | System for diagnostic testing of liquid samples | |
| CN106323977A (en) | Mobile terminal-based color-change diagnosis test paper quantitative imaging system | |
| CN107240089B (en) | Method for detecting concentration of analyte | |
| WO2014178062A2 (en) | Method and system for analysing body fluids | |
| CN104198695A (en) | Method for analyzing developing result of colloidal gold test strip | |
| CN105044087B (en) | Urine examination device for fast detecting and method | |
| CN110072470A (en) | Method and apparatus for estimating device for testing ovulation date | |
| CN111060502A (en) | Method for detecting sample substance concentration based on test paper image recognition | |
| WO2018081142A1 (en) | Assessment and control of reagents in automated slide preparation | |
| US20240288414A1 (en) | Water quality testing method and water quality testing apparatus | |
| CN112964652A (en) | Rapid detection device, system and detection method for solution colorimetric analysis | |
| CN112595711A (en) | Titration end point determination device | |
| Bao et al. | A remote computing based point-of-care colorimetric detection system with a smartphone under complex ambient light conditions | |
| CN106525826A (en) | Molecule smart-phone rapid test method based on colorful dominant wavelength and complementary wavelength | |
| CN205374301U (en) | Measure device of solution strength | |
| CN102735683A (en) | Reading instrument of pH test paper | |
| CN114441512B (en) | Vaginal secretion detector, dry chemical detection device and dry chemical detection method | |
| KR20180055320A (en) | Method of water examination using household water examination kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190212 |
|
| RJ01 | Rejection of invention patent application after publication |