CN104198695A - Method for analyzing developing result of colloidal gold test strip - Google Patents
Method for analyzing developing result of colloidal gold test strip Download PDFInfo
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 82
- 238000000034 method Methods 0.000 title claims abstract description 45
- 238000012360 testing method Methods 0.000 title claims abstract description 23
- 238000011161 development Methods 0.000 claims description 52
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 50
- 239000010931 gold Substances 0.000 claims description 12
- 229910052737 gold Inorganic materials 0.000 claims description 12
- 238000012545 processing Methods 0.000 claims description 6
- 238000012113 quantitative test Methods 0.000 claims description 6
- 238000004364 calculation method Methods 0.000 claims description 5
- 230000008878 coupling Effects 0.000 claims description 4
- 238000010168 coupling process Methods 0.000 claims description 4
- 238000005859 coupling reaction Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 12
- 229960001399 clenbuterol hydrochloride Drugs 0.000 abstract description 6
- OPXKTCUYRHXSBK-UHFFFAOYSA-N clenbuterol hydrochloride Chemical compound Cl.CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 OPXKTCUYRHXSBK-UHFFFAOYSA-N 0.000 abstract description 6
- 238000004445 quantitative analysis Methods 0.000 abstract description 2
- 229960001117 clenbuterol Drugs 0.000 description 4
- STJMRWALKKWQGH-UHFFFAOYSA-N clenbuterol Chemical compound CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 STJMRWALKKWQGH-UHFFFAOYSA-N 0.000 description 4
- 102000014914 Carrier Proteins Human genes 0.000 description 3
- 108010078791 Carrier Proteins Proteins 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 241001270131 Agaricus moelleri Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000048 adrenergic agonist Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 102000014974 beta2-adrenergic receptor activity proteins Human genes 0.000 description 1
- 108040006828 beta2-adrenergic receptor activity proteins Proteins 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000011524 similarity measure Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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Abstract
The invention provides a method for analyzing developing result of a colloidal gold test strip, belongs to the technical field of colloidal gold quantitative analysis and aims to solve the problem in the prior art of qualitative observation result after clenbuterol hydrochloride detection by colloidal gold. The method comprises the following steps: A, the relation of the developing level of a testing line and the concentration of the clenbuterol hydrochloride is established; B, a developing region of a controlled line, the developing region of the testing line and a background referential region are identified; C, developing grayness with the same size of pixel range for the developing region of the controlled line, the developing region of the testing line and the background referential region is acquired respectively; D, the developing degrees of the testing line and the controlled line are obtained and the developing level of the testing line is calculated; E, the concentration of the clenbuterol hydrochloride is obtained through the developing level of the testing line according to the established relation. Through the adoption of the method, as the clenbuterol hydrochloride is detected by the colloidal gold, the quantitative numerical value of the concentration of the clenbuterol hydrochloride can be obtained by analyzing the developing result of the testing line of the colloidal gold test strip.
Description
Technical field
The invention belongs to collaurum quantitative analysis tech field, relate to a kind of method to colloidal gold strip colour developing interpretation of result.
Background technology
Clenobuterol hydrochloride, is commonly called as " clenbuterol hydrochloride ", and it is a kind of selectivity beta-2-adrenoceptor agonist, has the function that regulates animal nerve excitement and endotrophic element thereof heavily to distribute.In feed, add clenobuterol hydrochloride and can make domestic animal growth rapidly, but how in animal body relict sediment and not being degraded of clenobuterol hydrochloride, people has eaten the domestic animal that contains clenobuterol hydrochloride, there will be toxic reaction, therefore the Chinese government give stricts orders statement and must not in the feed of domestic animal, use clenobuterol hydrochloride, and association area is inspected by random samples.
At present the detection mode of clenobuterol hydrochloride residue is had a variety ofly, wherein collaurum detection mode, because it is quick and convenient and the time is short, becomes the main mode of current use.Collaurum detection technique is a kind of low cost, convenient and swift, sensitive efficient, Fast Detection Technique that stability is strong, is widely used in food and medicine, medicine and hygiene fields.Adopt specific binding immunoassay reaction technology can produce corresponding colloid gold test strip.Colloidal gold strip is by the change color visual display detecting result of p-wire in test strips, and by contrast standard colorimetric card, range estimation obtains qualitative detection result.
Although what colloidal gold strip can Rapid Detection of Clenbuterol is residual, it estimates interpretation artificially, and what obtain is qualitative results, therefore there will be a lot of errors can not accurately obtain the residual concentration of clenobuterol hydrochloride.
For this reason, Chinese patent literature discloses colloidal gold strip and production and the using method of the quick clenobuterol hydrochloride of sxemiquantitative that application number is 02139704.X.Its semi-quantitative detection method is as follows: with the carrier protein couplet thing of colloid gold label clenobuterol hydrochloride, evenly be sprayed on glass fibre paper, the carrier protein antiserum of anti-clenobuterol hydrochloride is to horizontal stripe shape and is fixed at many levels in chromatographic film, Article 1 antibody concentration is just neutralized completely with the carrier protein couplet thing of collaurum institute mark.In the time that detection sample is negative, test strip only shows a band, in the time containing clenobuterol hydrochloride in detection sample, demonstrates multi-ribbon, and band is more, and the clenobuterol hydrochloride concentration in sample is higher.Although this patent can be to a certain extent according to the content of clenobuterol hydrochloride in how many judgement samples of band, still by people for going range estimation to observe, can there is error and can not accurately read the content of clenobuterol hydrochloride.
Summary of the invention
The present invention is directed to existing technology and have the problems referred to above, proposed a kind of method to colloidal gold strip colour developing interpretation of result, the method technical matters to be solved is the quantitative value that after how realizing colloidal gold strip clenobuterol hydrochloride being detected, colloidal gold strip p-wire colour developing interpretation of result is drawn clenobuterol hydrochloride concentration.
The present invention realizes by following technical proposal: a kind of method to colloidal gold strip colour developing interpretation of result, this method is marked the image of the image acquisition units collection colloidal gold strip of readout instrument by gold, and by the control chip of gold mark readout instrument, colloidal gold test line colour developing result in image is carried out to quantitative test, it is characterized in that, this method comprises the following steps:
A, the default calculation equation that the colour developing level of p-wire is converted into corresponding clenobuterol hydrochloride concentration;
The color development area of B, the color development area that identifies control line in colloidal gold strip image, p-wire and reference background region;
C, in the color development area of p-wire, obtain pixel coverage one, in the color development area of control line, obtain pixel coverage two, in reference background region, obtain pixel coverage three, described pixel coverage one, pixel coverage two and pixel coverage three are big or small identical, the gray scale of the each pixel in pixel coverage one is added the colour developing gray scale as p-wire, the gray scale of the each pixel in pixel coverage two is added the colour developing gray scale as control line, and the gray scale of the each pixel in pixel coverage three is added to the colour developing gray scale of reference zone as a setting;
D, obtain the colour developing degree of p-wire by the colour developing gray scale of the colour developing gray scale subtracting background reference zone of p-wire, with the colour developing degree of the controlled line of colour developing gray scale of the colour developing gray scale subtracting background reference zone of control line, by the colour developing level that is compared to p-wire of the colour developing degree of the colour developing degree of p-wire and control line.
E, the colour developing level of the p-wire obtaining is updated in default equation, reads out the concentration of current clenobuterol hydrochloride after colloidal gold strip detects.
Read instrument colloidal gold strip by gold mark and carry out image acquisition, afterwards the image of colloidal gold strip is processed.Set up a p-wire colour developing level equation corresponding with clenobuterol hydrochloride concentration, this equation can accurately calculate by the colour developing level of p-wire the concentration of clenobuterol hydrochloride, realizes the quantitative test of colloidal gold test line colour developing result.P-wire and the colour developing level that the ratio of the colour developing degree of control line is p-wire, therefore need first to obtain the colour developing degree of two lines.Determine the color development area of p-wire and control line, in the color development area of two lines, obtain respectively the pixel coverage of formed objects, be respectively pixel coverage one and pixel coverage two, in reference background region, obtain again the pixel coverage three of formed objects, the colour developing gray scale of the colour developing gray scale of pixel coverage one and pixel coverage two is subtracted each other to the colour developing degree that draws p-wire and control line with the colour developing gray scale of pixel coverage three respectively, thereby draw the colour developing level of p-wire.This method obtains the colour developing level of p-wire and the colour developing level of p-wire is updated in specific equation by colloidal gold strip image is processed, converse the concentration of clenobuterol hydrochloride, thereby realize the concentration numerical value to accurately drawing clenobuterol hydrochloride after colloidal gold strip p-wire colour developing interpretation of result.
In the method for above-mentioned interpretation of result that colloidal gold strip is developed the color, in described steps A, default equation is y=0.026x
2-0.1964x+1.025, the colour developing level that wherein y is p-wire, the concentration that x is clenobuterol hydrochloride.Just can draw the concentration numerical value of clenobuterol hydrochloride comparatively accurately by this equation drawing after the colour developing level of p-wire, complete the quantitative test of the colour developing to p-wire.
In the method for above-mentioned interpretation of result that colloidal gold strip is developed the color, in described step B, adopt the central point of the color development area the controlled line color development area that identify control line based on Feature Correspondence Algorithm in colloidal gold strip image, obtained the central point of the color development area of p-wire by the central point of the color development area of control line to constant pixel distance of left, obtained the color development area of p-wire by the central point of the color development area of p-wire.Feature Correspondence Algorithm is the method comparatively widely of using in image is processed, thereby draws control line by this algorithm.Because the central point distance of p-wire in test strips in reality and control line is constant dimensions, therefore after making a video recording, be also constant pixel distance between the two, this pixel distance is the pixel distance of measuring Conversion Calculation and go out both central points by the colloidal gold strip image people who obtains, and repeatedly calculates and averages.
Above-mentioned to the method for colloidal gold strip colour developing interpretation of result in, unidentified go out when the color development area of control line, do not carry out next step image processing and send the not prompting of the collaurum image of coupling.Unidentified go out the color development area of control line show that current collaurum breaks down, need not continue chromogenic assay simultaneously prompting personnel change.
In the method for above-mentioned interpretation of result that colloidal gold strip is developed the color, the central point using the mid point of the distance of the color development area central point of the color development area central point of p-wire and control line as pixel coverage three.Determine after the color development area of p-wire and control line, the mid point of the line of selected both central points, as the central point of pixel coverage three, positions the pixel that need to choose reference background region like this.
In the method for above-mentioned interpretation of result that colloidal gold strip is developed the color, in described step C, the central point of pixel coverage one is identical with the color development area central point of p-wire, and the central point of pixel coverage two is identical with the color development area central point of control line.Above-mentioned action makes the central area that obtains the color development area that is respectively p-wire and control line of pixel coverage one and pixel coverage two, make to obtain out as the color feature in color range with better p-wire and control line.
In the method for above-mentioned interpretation of result that colloidal gold strip is developed the color, in described step C, by the gray scale of the each pixel in pixel coverage one and threshold value comparison, the gray scale that is greater than the pixel of threshold value is added the colour developing gray scale as p-wire, by the gray scale of the each pixel in pixel coverage two and threshold value comparison, the gray scale that is greater than the pixel of threshold value is added the colour developing gray scale as control line.May there is the incomplete situation that develops the color in colloidal gold test line, same control line is also likely in the situation that exists part not develop the color, therefore by by pixel each with threshold value comparison, make not have the pixel developing the color to be rejected, thus make the colour developing interpretation of result of p-wire more accurate.
In the method for above-mentioned interpretation of result that colloidal gold strip is developed the color, described threshold value is the gray-scale value of any one pixel in reference background region in colloidal gold strip image.When the color development area of p-wire develops the color when incomplete, do not have the place of colour developing identical with the pixel in reference background region, therefore select the gray-scale value of any one pixel in reference background region as threshold value.
In the method for above-mentioned interpretation of result that colloidal gold strip is developed the color, in described step e, the concentration of the clenobuterol hydrochloride reading out is shown by display screen.The concentration of the clenobuterol hydrochloride of reading is shown and informed testing staff by display screen.
In the method for above-mentioned interpretation of result that colloidal gold strip is developed the color, the concentration of the clenobuterol hydrochloride reading out reading is stored, and can support and host computer mutual information.After the concentration of clenobuterol hydrochloride is stored, can in host computer, read and file, be convenient to testing staff's subsequent operation.
Compared with prior art, the present invention has the following advantages:
1, the present invention obtains the colour developing level of p-wire by colloidal gold strip is carried out to image processing, for the colour developing result quantitative test to p-wire provides the data that develop the color accurately.
2, the present invention has set up the relational model of colour developing level and the Clenbuterol concentration of p-wire, thereby accurately draws the concentration of Clenbuterol.
3, the present invention does not need by artificial judgement, and just accurately numerical value is read, and has improved the detection of the concentration to Clenbuterol and has supported that batch detection has higher marketing prospect.
Brief description of the drawings
Fig. 1 is workflow schematic diagram of the present invention.
Embodiment
Be below specific embodiments of the invention, and by reference to the accompanying drawings technical scheme of the present invention be further described, but the present invention is not limited to these embodiment.
As shown in Figure 1, a kind of method to colloidal gold strip colour developing interpretation of result, this method is marked the image of the image acquisition units collection colloidal gold strip of readout instrument by gold, and by the control chip of gold mark readout instrument, colloidal gold test line colour developing result in image is carried out to quantitative test, this method comprises the following steps:
Steps A, the default calculation equation that the colour developing level of p-wire is converted into corresponding clenobuterol hydrochloride concentration, default equation is y=0.026x
2-0.1964x+1.025, the colour developing level that wherein y is p-wire, the concentration that x is clenobuterol hydrochloride;
Step B, identify the color development area of control line in colloidal gold strip image, the color development area of p-wire and reference background region, adopt the central point of the color development area the controlled line color development area that identify control line based on Feature Correspondence Algorithm in colloidal gold strip image, obtained the central point of the color development area of p-wire to constant pixel distance of left by the central point of the color development area of control line, obtained the color development area of p-wire by the central point of the color development area of p-wire, unidentified go out when the color development area of control line, do not carry out next step image processing and send the not prompting of the collaurum image of coupling,
Step C, in the color development area of p-wire, obtain pixel coverage one, in the color development area of control line, obtain pixel coverage two, in reference background region, obtain pixel coverage three, described pixel coverage one, pixel coverage two is identical with pixel coverage three sizes, the gray scale of the each pixel in pixel coverage one is added the colour developing gray scale as p-wire, the gray scale of the each pixel in pixel coverage two is added the colour developing gray scale as control line, the gray scale of the each pixel in pixel coverage three is added to the colour developing gray scale of reference zone as a setting, central point using the mid point of the distance of the color development area central point of the color development area central point of p-wire and control line as pixel coverage three, the central point of pixel coverage one is identical with the color development area central point of p-wire, the central point of pixel coverage two is identical with the color development area central point of control line, by the gray scale of the each pixel in pixel coverage one and threshold value comparison, the gray scale that is greater than the pixel of threshold value is added the colour developing gray scale as p-wire, by the gray scale of the each pixel in pixel coverage two and threshold value comparison, the gray scale that is greater than the pixel of threshold value is added the colour developing gray scale as control line, and threshold value is the gray-scale value of any one pixel in reference background region in colloidal gold strip image,
Step D, obtain the colour developing degree of p-wire by the colour developing gray scale of the colour developing gray scale subtracting background reference zone of p-wire, with the colour developing degree of the controlled line of colour developing gray scale of the colour developing gray scale subtracting background reference zone of control line, by the colour developing level that is compared to p-wire of the colour developing degree of the colour developing degree of p-wire and control line;
Step e, the colour developing level of the p-wire obtaining is updated in default equation, read out the concentration of current clenobuterol hydrochloride after colloidal gold strip detects, the concentration of the clenobuterol hydrochloride reading out is shown by display screen, and the concentration of the clenobuterol hydrochloride reading out reading is stored, and can support and host computer mutual information.
P-wire colour developing and control line develop the color exist certain associated, and control line is identical with p-wire color condition, for example illumination, temperature etc., therefore control line colored intensity is incorporated in result judgement, can effectively judge testing result, therefore, in this method, the colour developing level of setting up p-wire is carried out the color feature of characterization test line comprehensively.
In step B, need to identify the color development area of the color development area of p-wire, control line and reference background region, because the position of p-wire is different in different collaurums, but in actual conditions, the central point of control line and p-wire distance is a steady state value, and the width length of the lines of control line is certain, therefore first identify control line.By adopting the central point of the color development area the controlled line color development area that identify control line based on Feature Correspondence Algorithm in colloidal gold strip image.Process is as follows: directly utilize all available gradation of image information can improve estimated accuracy, therefore adopt the matching characteristic algorithm of original gray value, control line background colour developing is around dark, whole control line is done to as a whole identification, all pixel gray scales and as feature in control line, therefore masterplate similarity measure elect as in matching stencil all pixels and, masterplate is the color development area of default control line.Again by exhaustive search method, by masterplate translation in whole colloidal gold strip image, carry out correspondence search, figure under masterplate covers is subgraph, by the pixel grey scale of masterplate with the pixel grey scale of subgraph with compare, the more little more coupling of both differences, the region of choosing difference minimum is defined as the color development area of control line, length and width by control line are fixed, therefore the central point of controlled line.
In step C, in the color development area of p-wire, obtain respectively pixel coverage one, in the color development area of control line, obtain pixel coverage two, in reference background region, obtain pixel coverage three, due to three's pixel coverage need to carry out pixel grey scale and calculating, therefore select the pixel coverage of formed objects.In order better to select the color feature of p-wire and control line, therefore the central point of pixel coverage is identical with the central point of corresponding color development area.Pixel coverage obtain the size that should be less than or equal to color development area, and it is better more to approach the size of color development area.
This method reads instrument by gold mark and gathers colloidal gold strip image and image is processed.Gold mark reads instrument and comprises housing, is provided with the image acquisition units for gathering colloidal gold strip colour developing image and draws the control chip of clenobuterol hydrochloride concentration numerical value for the image gathering is carried out to Treatment Analysis in housing.Image acquisition units comprises the light tool shell of a sealing, on light tool shell, offer the slot for inserting colloidal gold strip, in light tool shell, being provided with provides the LED of light source lamp and for obtaining the cmos digital imageing sensor of colloidal gold strip colour developing image.On housing, offer the mouth of stepping down, light tool shell be arranged on housing step down mouthful place and slot mouth with step down mouthful just rightly, surface of shell is provided with display screen, CMOS camera connects the input end of controller, display screen connects the output terminal of controller.
Carry out the explanation to workflow of the present invention by detecting the clenobuterol hydrochloride mark-on solution that the concentration of pure feminine gender is 1ng/ml below:
The clenobuterol hydrochloride mark-on solution that needs are detected splashes on colloidal gold strip, after 3 minutes, colloidal gold strip is inserted into gold mark completely and reads in the slot of instrument, cmos digital imageing sensor obtains the image of colloidal gold strip and sends to control chip to process.In control chip, be preset with the calculation equation that the colour developing level of p-wire is converted into corresponding clenobuterol hydrochloride concentration, default equation is y=0.026x
2-0.1964x+1.025, the colour developing level that wherein y is p-wire, the concentration that x is clenobuterol hydrochloride.Sum up above-mentioned equation via test of many times and for the judgement of test findings.
As follows to the main process of image processing by control chip: to identify the color development area of control line in colloidal gold strip image, color development area and the reference background region of p-wire, unidentified go out when the color development area of control line, do not carry out next step image processing and send the not prompting of the collaurum image of coupling.In the color development area of p-wire, obtain pixel coverage one, in the color development area of control line, obtain pixel coverage two, in reference background region, obtain pixel coverage three.By the gray scale of the each pixel in pixel coverage one and threshold value comparison, the gray scale that is greater than the pixel of threshold value is added the colour developing gray scale as p-wire, by the gray scale of the each pixel in pixel coverage two and threshold value comparison, the gray scale that is greater than the pixel of threshold value is added the colour developing gray scale as control line, the gray scale of the each pixel in pixel coverage three is added to the colour developing gray scale of reference zone as a setting, and threshold value is the gray-scale value of any one pixel in reference background region in colloidal gold strip image.
Obtain the colour developing degree of p-wire by the colour developing gray scale of the colour developing gray scale subtracting background reference zone of p-wire, with the colour developing degree of the controlled line of colour developing gray scale of the colour developing gray scale subtracting background reference zone of control line, by the colour developing level that is compared to p-wire of the colour developing degree of the colour developing degree of p-wire and control line.Be 0.86 by the colour developing level that draws current colloidal gold strip after above-mentioned processing procedure.Y=0.86 is updated to y=0.026x
2in-0.1964x+1.025, obtain x=0.96, the concentration that is to say the clenobuterol hydrochloride quantitatively reading by the colour developing interpretation of result to p-wire is 0.96ng/ml.The clenobuterol hydrochloride mark-on solution of 1ng/ml is carried out to repetition to be measured for five times, obtain respectively the colour developing level of p-wire: 0.86,0.85,0.84,0.87,0.86, thus the concentration that reads clenobuterol hydrochloride is respectively: 0.96ng/ml, 1.03ng/ml, 1.10ng/ml, 0.89ng/ml, 0.96ng/ml.By finding out that above the concentration error that reads clenobuterol hydrochloride drawing by above-mentioned equation is less, can comparatively accurately the p-wire colour developing result of colloidal gold strip be analyzed quantitatively and be read.
The clenobuterol hydrochloride mark-on solution that is 2ng/ml to the concentration of pure feminine gender carries out repetition and measures for five times, obtain respectively the colour developing level of p-wire: 0.74,0.75,0.73,0.73,0.75, thus the concentration that reads clenobuterol hydrochloride is respectively: 1.95ng/ml, 1.85ng/ml, 2.06ng/ml, 2.06ng/ml, 1.85ng/ml.
The clenobuterol hydrochloride mark-on solution that is 3ng/ml to the concentration of pure feminine gender carries out repetition and measures for five times, obtain respectively the colour developing level of p-wire: 0.68,0.66,0.68,0.67,0.67, thus the concentration that reads clenobuterol hydrochloride is respectively: 2.78ng/ml, 3.30ng/ml, 2.78ng/ml, 2.99ng/ml, 2.99ng/ml.
As known from the above, the calculation equation that the colour developing level of the p-wire that this method is set up is converted into corresponding clenobuterol hydrochloride concentration can be used for any clenobuterol hydrochloride mark-on solution to detect, and error is less, and the data that quantitatively draw are accurate.
Finally the concentration of the clenobuterol hydrochloride reading out is shown by display screen, and the concentration of the clenobuterol hydrochloride reading out reading is stored, and can support and host computer mutual information.
Specific embodiment described herein is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various amendments or supplement or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present invention or surmount the defined scope of appended claims.
Claims (10)
1. the method to colloidal gold strip colour developing interpretation of result, this method is marked the image of the image acquisition units collection colloidal gold strip of readout instrument by gold, and by the control chip of gold mark readout instrument, colloidal gold test line colour developing result in image is carried out to quantitative test, it is characterized in that, this method comprises the following steps:
A, the default calculation equation that the colour developing level of p-wire is converted into corresponding clenobuterol hydrochloride concentration;
The color development area of B, the color development area that identifies control line in colloidal gold strip image, p-wire and reference background region;
C, in the color development area of p-wire, obtain pixel coverage one, in the color development area of control line, obtain pixel coverage two, in reference background region, obtain pixel coverage three, described pixel coverage one, pixel coverage two and pixel coverage three are big or small identical, the gray scale of the each pixel in pixel coverage one is added the colour developing gray scale as p-wire, the gray scale of the each pixel in pixel coverage two is added the colour developing gray scale as control line, and the gray scale of the each pixel in pixel coverage three is added to the colour developing gray scale of reference zone as a setting;
D, obtain the colour developing degree of p-wire by the colour developing gray scale of the colour developing gray scale subtracting background reference zone of p-wire, with the colour developing degree of the controlled line of colour developing gray scale of the colour developing gray scale subtracting background reference zone of control line, by the colour developing level that is compared to p-wire of the colour developing degree of the colour developing degree of p-wire and control line.
E, the colour developing level of the p-wire obtaining is updated in default equation, reads out the concentration of current clenobuterol hydrochloride after colloidal gold strip detects.
2. the method to colloidal gold strip colour developing interpretation of result according to claim 1, is characterized in that, in described steps A, default equation is y=0.026x
2-0.1964x+1.025, the colour developing level that wherein y is p-wire, the concentration that x is clenobuterol hydrochloride.
3. the method to colloidal gold strip colour developing interpretation of result according to claim 2, it is characterized in that, in described step B, adopt the central point of the color development area the controlled line color development area that identify control line based on Feature Correspondence Algorithm in colloidal gold strip image, obtained the central point of the color development area of p-wire by the central point of the color development area of control line to constant pixel distance of left, obtained the color development area of p-wire by the central point of the color development area of p-wire.
4. the method to colloidal gold strip colour developing interpretation of result according to claim 3, is characterized in that, unidentified go out when the color development area of control line, do not carry out next step image processing and send the not prompting of the collaurum image of coupling.
5. according to the method to colloidal gold strip colour developing interpretation of result described in claim 3 or 4, it is characterized in that the central point using the mid point of the distance of the color development area central point of the color development area central point of p-wire and control line as pixel coverage three.
6. the method to colloidal gold strip colour developing interpretation of result according to claim 5, it is characterized in that, in described step C, the central point of pixel coverage one is identical with the color development area central point of p-wire, and the central point of pixel coverage two is identical with the color development area central point of control line.
7. the method to colloidal gold strip colour developing interpretation of result according to claim 6, it is characterized in that, in described step C, by the gray scale of the each pixel in pixel coverage one and threshold value comparison, the gray scale that is greater than the pixel of threshold value is added the colour developing gray scale as p-wire, by the gray scale of the each pixel in pixel coverage two and threshold value comparison, the gray scale that is greater than the pixel of threshold value is added the colour developing gray scale as control line.
8. the method to colloidal gold strip colour developing interpretation of result according to claim 7, is characterized in that, described threshold value is the gray-scale value of any one pixel in reference background region in colloidal gold strip image.
9. the method to colloidal gold strip colour developing interpretation of result according to claim 8, is characterized in that, in described step e, the concentration of the clenobuterol hydrochloride reading out is shown by display screen.
10. the method to colloidal gold strip colour developing interpretation of result according to claim 9, is characterized in that, the concentration of the clenobuterol hydrochloride reading out reading is stored, and can support and host computer mutual information.
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