CN106645706A - Method for quantitatively analyzing colloidal gold detection reagent by mobile terminal - Google Patents
Method for quantitatively analyzing colloidal gold detection reagent by mobile terminal Download PDFInfo
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- CN106645706A CN106645706A CN201511009441.9A CN201511009441A CN106645706A CN 106645706 A CN106645706 A CN 106645706A CN 201511009441 A CN201511009441 A CN 201511009441A CN 106645706 A CN106645706 A CN 106645706A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention relates to a mobile-terminal analysis method capable of carrying out quantifying treatment on detection results of a colloidal gold detection reagent and provides a bran-new method for carrying out quantitative analysis on the colloidal gold detection reagent by a mobile terminal. The method comprises the steps: the mobile terminal is used for carrying out acquisition and reading on coded information and biological information on the colloidal gold detection reagent, and the information is synchronized to a cloud side server; the cloud side server receives the information, starts to carry out computational analysis and synchronizes an analyzed result to the mobile terminal, so that users can rapidly and accurately know the detection results and significance. According to the method, the problem that the existing colloidal gold quantitative analyzers are expensive in price, can only be applied to hospitals or special laboratories and are complicated in operation is solved. By implementing the method, the users can rapidly, simply and conveniently acquire the detection results and significance.
Description
Technical field
The present invention relates to a kind of method for carrying out quantification treatment to gold-immunochromatographyreagent reagent for assay testing result by mobile terminal.
Background technology
At present, antigen, antibody, albumen, virus, micro- is detected in traditional food security laboratory and Lab of Clinical Medicine
Biology, medicine, compound etc. are more to be detected using gold-immunochromatographyreagent reagent for assay, and testing result relies primarily on perusal, as a result
Can only be qualitative, it is impossible to carry out data analysiss, the error ratio that deviation and anthropic factor are caused is larger, and testing result lacks objectivity.
So gold-immunochromatographyreagent reagent for assay quantitative analysis method is developed in market in urgent need.
Dot immune gold filtration assay is a kind of Fast Detection Technique of rising in recent years, is anti-using the antigen-antibody principle that combines
A kind of detection technique answered.The technology have quick, sensitivity height, high specificity, good stability, result simple to operate it is accurate,
It is easy to the advantages of judging, for having higher-value to Lab of Clinical Medicine medical diagnosis on disease from food safety laboratory research.
With the progress of science and technology, in institute's generation, is just progressively read with instrument and equipment using the traditional method of visual results
Replace, the impact that result avoids to a certain extent artificial subjective factor is read with instrument and equipment, so that testing result is more
Accurately.
Current golden scalar quantity analyser reads the principle of result, typically in certain signal source (such as：It is monochromatic light, glimmering
Light etc.) in the presence of so that tracer reflects visible ray or sends the signal that fluorescence etc. can be detected, and is painted by sensor scan
The intensity curves of tracer processed carry out data analysiss；Or after being imaged to detection zone, then image is identified point
Analysis.
Fig. 1 is colloidal gold immunochromatographimethod detection card schematic diagram.In general, detection card 1 mainly includes well 2 and sees
Window 3 is examined, observation window 3 is provided with nature controlling line 4 (i.e. C lines) and detection line 5 (i.e. T lines).At present, the result point of detection card
Analysis method is the judgement that detects by an unaided eye, i.e., add on the well 2 and certain hour is waited after sample, and operator detects by an unaided eye matter
Control the color regime of line 4 and detection line 5 to judge testing result.This is a kind of method of Macroscopic analysis, exist resolution it is low,
Can only it is qualitative, can not be quantitative, testing result rely on the subjective experience of operator, detection have very big subjectivity, False Rate it is higher,
Testing result can only hand-kept, it is not easy to maintain, be only applicable to professional and the drawback such as use.Therefore, gold-immunochromatographyreagent reagent for assay
Mobile terminal quantitative analysis method is born with regard to this.
Fig. 2 is colloidal gold immunochromatographydetection detection test paper schematic diagram.In general, colloidal gold test 6 mainly includes adding
Sample area 7 and observation area 8, observation area 8 is provided with nature controlling line 9 (i.e. C lines) and detection line 10 (i.e. T lines).At present, detect
The interpretation of result method of reagent paper is the judgement that detects by an unaided eye, i.e., wait certain hour, operator after adding sample on sample application zone 7
The color regime of nature controlling line 9 and the detection line 10 of detecting by an unaided eye is judging testing result.This is a kind of method of Macroscopic analysis, is deposited
Resolution it is low, can only it is qualitative, can not be quantitative, testing result rely on the subjective experience of operator, detection have it is greatly subjective
Property, False Rate are higher, testing result can only hand-kept, it is not easy to maintain, be only applicable to professional and the drawback such as use.Therefore,
The quantitative analysis method of gold-immunochromatographyreagent reagent for assay mobile terminal is born with regard to this.
Fig. 3 is colloidal gold immunity percolation detection card schematic diagram.In general, detection card 11 mainly includes well with observation
Window 12, well is provided with Quality Control point 13 (i.e. C points) and test point 14 (i.e. T points) with observation window 12.At present, detect
The interpretation of result method of card is the judgement that detects by an unaided eye, i.e., wait certain hour after adding sample on well and observation window 12,
Operator detect by an unaided eye Quality Control point 13 and test point 14 color regime judging testing result.This is a kind of Macroscopic analysis
Method, exist resolution it is low, can only it is qualitative, can not be quantitative, testing result rely on operator subjective experience, detection has
Greatly subjectivity, False Rate be higher, testing result can only hand-kept, it is not easy to maintain, be only applicable to professional and the disadvantage such as use
End.Therefore, gold-immunochromatographyreagent reagent for assay mobile terminal quantitative analysis method is born with regard to this.
Because traditional gold colloidal quantitative analysis instrument is mostly used in specialized laboratory, its is bulky, complex operation, right
Personnel and use environment require the defect such as higher, therefore general population is difficult to operate without professional training, it is difficult to which gold colloidal is quantitatively divided
The result that analyzer shows is analyzed.Just become this hence with a kind of analysis method of gold-immunochromatographyreagent reagent for assay of mobile terminal design
The key of invention.
The content of the invention
Present invention aim to overcome that above-mentioned technical deficiency, so as to provide a kind of mobile terminal quantitative analyses gold colloidal
The method of detectable so that using the mobile terminal of this method can quickly draw accurate, stable, reliable testing result and
Meaning.
To realize object above, a kind of method of mobile terminal quantitative analyses gold-immunochromatographyreagent reagent for assay of present invention description is described
Gold-immunochromatographyreagent reagent for assay has coding information.
Further, it is characterized by there is a coding information on detectable surface, coding information can for numeral, character, letter,
One-dimension code, Quick Response Code, three-dimension code etc., but not limited to this.
Further, corresponding coordinate figure in the coding information correspondence cloud server.
The present invention also provides a kind of mobile terminal, it is characterized by equipment is equipped with the program of image collecting function or based on other journeys
The program module of sequence, can direct or indirect connection the Internet with data-transformation facility.
Further, the mobile terminal be capable of achieving data syn-chronization one kind based on Android, IOS, Windows,
The equipment of the systems such as BlackBerry, Symbian, JAVA, but not limited to this.
Further, the mobile terminal can be the equipment such as mobile phone, panel computer, notebook computer, intelligent watch,
But not limited to this.
The present invention also provides a kind of cloud server, the cloud server, it is characterized by installing coding information and biological letter
Breath processing routine server, by receive mobile terminal synchronization coding information and bio information, carry out gradation of image process,
Analyze bio information, respective coordinates figure is found out by coding information；And detection data is calculated according to coordinate diagram, then by detection knot
Fruit and meaning are back to mobile terminal.
Beneficial effect
The above-mentioned technical proposal of the present invention has the advantages that, is conducive to user to pass through mobile terminal and gold colloidal is detected
Reagent carries out coding information and biomedical information acquisition, and synchrodata is analyzed to cloud server, can obtain testing result and
Meaning is simultaneously back to mobile terminal, realizes the possibility for quickly and easily obtaining testing result and meaning whenever and wherever possible.
Description of the drawings
Fig. 1 is traditional colloidal gold immunochromatographimethod detection card schematic diagram；
Fig. 2 is traditional colloidal gold immunochromatographydetection detection test paper schematic diagram；
Fig. 3 is traditional colloidal gold immunity percolation detection card schematic diagram；
Fig. 4 is that colloidal gold immunochromatographimethod of the present invention detects card schematic diagram；
Fig. 5 is colloidal gold immunochromatographydetection detection test paper schematic diagram of the present invention；
Fig. 6 is that colloidal gold immunity percolation of the present invention detects card schematic diagram；
Fig. 7 is the workflow schematic diagram of traditional gold scalar quantity analyser；
Fig. 8 is testing result intensity contrast schematic diagram of the present invention；
Fig. 9 is testing result coordinate graph of the present invention intention；
Figure 10 is mobile terminal quantitative analyses colloid gold reagent detection method workflow schematic diagram；
Specific embodiment
Embodiment one
Fig. 4 is colloidal gold immunochromatographimethod detection card schematic diagram of the present invention.Detection card 1 mainly includes well 2 and observation window
3, observation window 3 is provided with nature controlling line 4 (i.e. C lines) and detection line 5 (i.e. T lines).With the traditional colloid gold immune shown in Fig. 1
There is coding information 15 on chromatography detection card difference, 1 surface of detection card.
Fig. 5 is colloidal gold immunochromatographydetection detection test paper schematic diagram of the present invention.Colloidal gold test 6 mainly includes sample application zone
7 and observation area 8, observation area 8 is provided with nature controlling line 9 (i.e. C lines) and detection line 10 (i.e. T lines).With the biography shown in Fig. 2
System colloidal gold immunochromatographydetection detection test paper difference, there is coding information 15 on the surface of reagent paper 6.
Fig. 6 is colloidal gold immunity percolation detection card schematic diagram of the present invention.Detection card 11 mainly includes well and observation window
12, well is provided with Quality Control point 13 (i.e. C points) and test point 14 (i.e. T points) with observation window 12.With the biography shown in Fig. 3
System colloidal gold immunity percolation detection card difference, there is coding information 15 on 11 surfaces of detection card.
In order to better illustrate the present invention, in embodiments of the present invention, coding information includes：
(1) manufacturing enterprise's title；
(2) the corresponding coordinate diagram of cloud server；
(3) batch number；
(4) date of manufacture；
(5) name to be checked claims.
Embodiment two
Gold-immunochromatographyreagent reagent for assay is operated to specifications, experiment is adopted at the appointed time after finishing using mobile terminal
Collection bio information, being synchronized to cloud server carries out Data Analysis Services.
In order to the present invention is better described, the mobile terminal is equipped with the program of image collecting function or based on other programs
Program module, be capable of achieving with one kind of cloud server data syn-chronization based on Android, IOS, Windows, BlackBerry,
The equipment of the systems such as Symbian, JAVA, but not limited to this.
Embodiment three
Cloud server receives the information of mobile terminal synchronization, and colour picture is converted to into gray scale picture, according to detection zone
Domain T optics gray values, Quality Control region C optics gray values, the optics gray value of contrast district are analyzed calculating, obtain numerical value.
The numerical value of acquisition is found out in cloud server corresponding coordinate diagram so as to drawing testing result and meaning and returning by coding information
To mobile terminal.
In order to the present invention is better described, the cloud server is the server for installing Bioinformatics program, is passed through
The coding information and bio information of mobile terminal synchronization are received, gradation of image process, analysis of biological information is carried out, coding letter is found out
Corresponding coordinate diagram is ceased, the testing result for obtaining and meaning are back to into mobile terminal with analysis by calculating.
Detectable Bioinformatics method includes：Coordinate diagram is according to the gold colloidal obtained under laboratory standard environment
Concentrations versus' figure of reagent optics gray value x, according to 255 grades of differentiations of gray scale, 255 concentration value y of correspondence.Detectable is examined
Survey region T, Quality Control region C colour developing constantly to deepen with time lengthening color.After stable reaction, Quality Control region C optics is read
Gray value c, detection zone T optics gray value t1, contrast district optics gray value t2.And c, t1, t2 are designated as respectively, count
Calculate its concentration value y correspondence optics gray value x, x=255-t2+t1.Using x values as quantitative analysis results, and exist according to the result
Correspondence y values are found out in coordinate diagram, for the concentration value of rational judgment detected material, i.e. testing result.
Finally it should be noted that：Above example, can be to this only to illustrative and not limiting technical scheme
It is bright to modify or equivalent, any modification or partial replacement without departing from the spirit and scope of the present invention, it all should contain
Cover in the claims of the present invention.
Claims (9)
1. a kind of method of mobile terminal quantitative analyses gold-immunochromatographyreagent reagent for assay, it is characterised in that：By mobile terminal, gold colloidal detection examination
Agent, cloud server, data analysis processing method are constituted, and its operating procedure is as follows：
Step 1：Gold-immunochromatographyreagent reagent for assay operation is carried out according to product description, waits question response to terminate standby；
Step 2：Coding information and biomedical information acquisition are carried out to the gold-immunochromatographyreagent reagent for assay that reaction terminates using mobile terminal；
Step 3：Mobile terminal synchronization coding information and bio information are to cloud server；
Step 4：Cloud server obtains testing result and meaning by Data Analysis Services；
Step 5：Cloud server returns testing result and meaning to mobile terminal.
2. the gold-immunochromatographyreagent reagent for assay according to claim 1, it is characterized by with gold colloidal, collargol, latex beadses, magnetic bead, glimmering
Light element etc. detects specific antigen, antibody, albumen, virus, microorganism, antibiotic, medicine, compound as tracer
Deng, but not limited to this.
3. the gold-immunochromatographyreagent reagent for assay according to claim 1, it is characterized by immunochromatographic method, immunity percolation method etc., reagent is detection
Card, Test paper, detection pen, detection bucket etc., but not limited to this.
4. the gold-immunochromatographyreagent reagent for assay according to claim 1, it is characterized by there is coding information on detectable surface, for recognition detection
Respective coordinates figure is found out in reagent type and beyond the clouds server.
5. there is coding information on gold-immunochromatographyreagent reagent for assay surface according to claim 4, can for numeral, character, letter, one-dimension code,
Quick Response Code, three-dimension code etc., but not limited to this.
6. the mobile terminal according to claim 1, it is characterized by program of the equipment equipped with coding information and biomedical information acquisition function or
Based on the program module of other programs, be capable of achieving with one kind of cloud server data syn-chronization based on Android, IOS, Windows,
The equipment of the systems such as BlackBerry, Symbian, JAVA, but not limited to this.
7. the mobile terminal according to claim 6, can be the equipment such as mobile phone, panel computer, notebook computer, intelligent watch,
But not limited to this.
8. the cloud server according to claim 1, it is characterized by the server of coding information and Bioinformatics program is installed,
The coding information and bio information of acquisition are drawn into testing result and meaning by series of algorithms, and is back to mobile terminal.
9. a kind of method of mobile terminal quantitative analyses gold-immunochromatographyreagent reagent for assay according to claim 1, methods described includes, mobile
Terminal capturing and coding information and bio information are simultaneously synchronized to cloud server, and cloud server is looked for according to coding information and bio information
Go out respective coordinates map analysis and go out testing result, then testing result and meaning are back to into mobile terminal.
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| CN201511009441.9A CN106645706A (en) | 2015-12-29 | 2015-12-29 | Method for quantitatively analyzing colloidal gold detection reagent by mobile terminal |
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Cited By (8)
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| CN107590413A (en) * | 2017-09-15 | 2018-01-16 | 胡晓甬 | Quick Response Code reagent detects carrier and its manufacture method, detection method, detecting system |
| CN107644237A (en) * | 2017-08-28 | 2018-01-30 | 天津派普大业仪器科技有限公司 | Multistage intelligent immunochromatography records interpreting system and its interpretation method |
| CN108387574A (en) * | 2018-02-28 | 2018-08-10 | 刘卓 | A kind of component detection method and system |
| CN108982450A (en) * | 2018-07-24 | 2018-12-11 | 上海雄图生物科技有限公司 | A kind of food safety fluorescence immunoassay quantifies poct detection system |
| CN111157673A (en) * | 2018-11-08 | 2020-05-15 | 江苏达骏生物科技有限公司 | Quantitative detection method and device |
| CN113156111A (en) * | 2021-04-16 | 2021-07-23 | 上海雄图生物科技有限公司 | Time-resolved fluoroimmunoassay quantitative POCT detection system for antibiotics in feed |
| WO2022041233A1 (en) * | 2020-08-31 | 2022-03-03 | 余伟华 | Pathogen and antibody detection system for mobile terminal |
| CN114911987A (en) * | 2022-05-24 | 2022-08-16 | 无锡市第五人民医院 | Autonomous analysis method and system for detection reagent strip |
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