CN108562750A - Blotting membrane item result interpretation method - Google Patents
Blotting membrane item result interpretation method Download PDFInfo
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- CN108562750A CN108562750A CN201810553862.5A CN201810553862A CN108562750A CN 108562750 A CN108562750 A CN 108562750A CN 201810553862 A CN201810553862 A CN 201810553862A CN 108562750 A CN108562750 A CN 108562750A
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- band
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- average color
- spleen
- yang
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
Abstract
The invention discloses a kind of blotting membrane item result interpretation method, one, the diagnostic reagent film item of protein immunoblot instrument is shot, obtain diagnostic reagent film image；Diagnostic reagent film item includes three Quality Control bands and multiple marker bands；Three Quality Control bands are respectively strong positive band, middle-jiao yang, function of the spleen and stomach band and weak positive band；Each marker band in multiple marker bands corresponds to a different markers respectively；Two, sectional drawing is carried out to strong positive band, middle-jiao yang, function of the spleen and stomach band, weak positive band and each marker band in diagnostic reagent film image respectively；Three, average color is sought respectively to each image intercepted in second step;Four, the average color of each marker band is compared with the average color of strong positive band, middle-jiao yang, function of the spleen and stomach band, weak positive band respectively, show that the marker yin and yang attribute marks.The invention has the advantages that by the yin and yang attribute of image recognition judgement symbol object, reaching makes interpretation method procedure, mass.
Description
Technical field
The present invention relates to Western blot detection human allergy sources, more particularly, to blotting membrane item result interpretation method.
Background technology
Immunoblotting is also known as Western blotting, be certain in the specific binding detection of complex sample according to antigen-antibody
The method of albumen.Blotting is a technology for being widely used in protein expression level in detection cell or tissue extract, this
Technology measures the protein level in biological sample by the combination of antibody and destination protein.Currently, existing Blot experiment
It is mostly manually operated by doctor, i.e.,：Sample-adding, reaction, cleaning, then observe by the naked eye interpretation blotting membrane item result.Pass through meat
Eye interpretation blotting membrane item is result it is required that doctor has higher professional standards and abundant experiment experience.Due to each doctor reality
The experience of testing has differences, therefore carries prodigious human factor to the accuracy of blotting membrane item result interpretation, and is unfavorable for interpretation
As a result digitlization preserves.Meanwhile result is checked comprising more than ten of marker band, doctor on protein immunoblot instrument film item
When, it can only one by one be checked by visually, efficiency is low.
Invention content
Present invention aims at a kind of blotting membrane item result interpretation method is provided, using image processing method, diagnosis is tried
The each marker band of agent film item carries out result interpretation, not only accurate but also convenient.
To achieve the above object, the present invention takes following technical proposals：
Blotting membrane item result interpretation method of the present invention, includes the following steps：
The first step first shoots the diagnostic reagent film item of protein immunoblot instrument, obtains diagnostic reagent film image；Institute
It includes three Quality Control bands and multiple marker bands to state diagnostic reagent film item；Three Quality Control bands are respectively strong positive item
Band, middle-jiao yang, function of the spleen and stomach band and weak positive band；Each marker band in multiple marker bands corresponds to different one respectively
A marker；
Second step, respectively to the strong positive band, middle-jiao yang, function of the spleen and stomach band, the weak positive band and each in the diagnostic reagent film image
Marker band carries out sectional drawing, and each image of interception is numbered and is preserved；
Third step seeks average color respectively to each described image intercepted in second step, i.e.,：Each pixel of image
Tri- values of RGB add up summation respectively, and last divided by number of pixels, calculation formula are：
；
Wherein：
Crgb:Average color；Cnr：The R channel values of nth pixel；Cng：The G channel values of nth pixel；Cnb：N-th of picture
The channel B value of element, n：The number of pixels of image;
4th step, by the average color of each marker band respectively with strong positive band, middle-jiao yang, function of the spleen and stomach band, weak positive band
Average color is compared；It, will when the average color of some marker band is more than the average color of strong positive band
The marker yin and yang attribute is labeled as strong sun +++；When the average color of some marker band is between strong positive band and middle-jiao yang, function of the spleen and stomach item
When the average color of band, which is labeled as middle-jiao yang, function of the spleen and stomach ++；When the average color of some marker band is situated between
When the average color of middle-jiao yang, function of the spleen and stomach band and weak positive band, by the marker yin and yang attribute labeled as weak sun+；When some marker item
When the average color of band is less than the average color of weak positive band, by the marker yin and yang attribute labeled as feminine gender --.
The described image format of shooting is JPG, BMP or PNG picture format.
The invention has the advantages that by the yin and yang attribute of image recognition judgement symbol object, the result figure of record diagnosis reagent membrane item
Picture, reaching makes interpretation method procedure, mass.Also, the result images of diagnostic reagent film item are preserved, after facilitating doctor
Continuous review.Meanwhile solving the deficiencies of doctor's unaided eye discrimination unstable result, require experience high, strongly professional.
Description of the drawings
Fig. 1 is the structural schematic diagram of diagnostic reagent film item of the present invention.
Specific implementation mode
It elaborates below in conjunction with the accompanying drawings to the embodiment of the present invention, the present embodiment before being with technical solution of the present invention
It puts and is implemented, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to down
State embodiment.
Blotting membrane item result interpretation method of the present invention, includes the following steps：
The first step first shoots the diagnostic reagent film item of protein immunoblot instrument, obtains diagnostic reagent film image, claps
The picture format taken the photograph is JPG, BMP or PNG picture format；Diagnostic reagent film item is as shown in Figure 1, include three 1 Hes of Quality Control band
17 marker bands 2；Three Quality Control bands 1 are respectively strong positive band, middle-jiao yang, function of the spleen and stomach band and weak positive band；17 markers
Each marker band in band 2 corresponds to a different markers respectively；
Second step, respectively to strong positive band, middle-jiao yang, function of the spleen and stomach band, weak positive band and each marker item in diagnostic reagent film image
Band carries out sectional drawing, and each image of interception is numbered and is preserved；
Third step seeks average color respectively to each image intercepted in second step, i.e.,：Each pixel of image RGB
Three values add up summation respectively, and last divided by number of pixels, calculation formula are：
；
Wherein：
Crgb:Average color；Cnr：The R channel values of nth pixel；Cng：The G channel values of nth pixel；Cnb：N-th of picture
The channel B value of element, n：The number of pixels of image;
The average color of each marker band is averaged with strong positive band, middle-jiao yang, function of the spleen and stomach band, weak positive band by the 4th step respectively
Color value is compared；When the average color of some marker band is more than the average color of strong positive band, by the mark
Will object yin and yang attribute is labeled as strong sun +++, i.e.,：（C1.R+ C1.G+ C1.B）<（C2.R+ C2.G+ C2.B）；Wherein：C1.R generations
Positive band color red color channel value, C1.G represent Qiang Yang band colors green channel values to table by force, and C1.B represents Qiang Yang band colors
Blue channel value；C2.R represents marker band color red color channel value, and C2.G represents marker band colors green channel value,
C2.B represents marker band color blue channel value.
When the average color of some marker band is between the average color of strong positive band and middle-jiao yang, function of the spleen and stomach band, by this
Marker yin and yang attribute is labeled as middle-jiao yang, function of the spleen and stomach ++；When the average color of some marker band is between middle-jiao yang, function of the spleen and stomach band and weak positive band
When average color, by the marker yin and yang attribute labeled as it is weak sun+；When the average color of some marker band is less than weak sun
When the average color of band, by the marker yin and yang attribute labeled as feminine gender.
Marker requires to select according to diagnosis, is typically chosen as human allergy source such as pollen, dandelion, bark, hamster secretion
Object, cat fur, dog skin, horsehair skin etc..
Claims (2)
1. a kind of blotting membrane item result interpretation method, it is characterised in that：Include the following steps：
The first step first shoots the diagnostic reagent film item of protein immunoblot instrument, obtains diagnostic reagent film image；Institute
It includes three Quality Control bands and multiple marker bands to state diagnostic reagent film item；Three Quality Control bands are respectively strong positive item
Band, middle-jiao yang, function of the spleen and stomach band and weak positive band；Each marker band in multiple marker bands corresponds to different one respectively
A marker；
Second step, respectively to the strong positive band, middle-jiao yang, function of the spleen and stomach band, the weak positive band and each in the diagnostic reagent film image
Marker band carries out sectional drawing, and each image of interception is numbered and is preserved；
Third step seeks average color respectively to each described image intercepted in second step, i.e.,：Each pixel of image
Tri- values of RGB add up summation respectively, and last divided by number of pixels, calculation formula are：
；
Wherein：
Crgb:Average color；Cnr：The R channel values of nth pixel；Cng：The G channel values of nth pixel；Cnb：N-th of picture
The channel B value of element, n：The number of pixels of image;
4th step, by the average color of each marker band respectively with strong positive band, middle-jiao yang, function of the spleen and stomach band, weak positive band
Average color is compared；It, will when the average color of some marker band is more than the average color of strong positive band
The marker yin and yang attribute is labeled as strong sun +++；When the average color of some marker band is between strong positive band and middle-jiao yang, function of the spleen and stomach item
When the average color of band, which is labeled as middle-jiao yang, function of the spleen and stomach ++；When the average color of some marker band is situated between
When the average color of middle-jiao yang, function of the spleen and stomach band and weak positive band, by the marker yin and yang attribute labeled as weak sun+；When some marker item
When the average color of band is less than the average color of weak positive band, by the marker yin and yang attribute labeled as feminine gender --.
2. blotting membrane item result interpretation method according to claim 1, it is characterised in that：The described image format of shooting is
JPG, BMP or PNG picture format.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110223290A (en) * | 2019-06-19 | 2019-09-10 | 深圳市亚辉龙生物科技股份有限公司 | Film appraisal procedure, device, computer equipment and storage medium |
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2018
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| CN102759617A (en) * | 2012-07-20 | 2012-10-31 | 无锡市东林科技发展有限责任公司 | Protein imprint automatic interpretation method and interpretation device thereof |
| CN104181295A (en) * | 2013-05-24 | 2014-12-03 | 南开大学 | Image processing method for quantitative analysis of multi-line colloidal gold test strip |
| CN104198695A (en) * | 2014-09-28 | 2014-12-10 | 杨伟群 | Method for analyzing developing result of colloidal gold test strip |
| CN107209934A (en) * | 2014-12-03 | 2017-09-26 | 文塔纳医疗系统公司 | Mthods, systems and devices for quantitative analysis heterogeneous biological marker profile |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110223290A (en) * | 2019-06-19 | 2019-09-10 | 深圳市亚辉龙生物科技股份有限公司 | Film appraisal procedure, device, computer equipment and storage medium |
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