CN106323977A - Mobile terminal-based color-change diagnosis test paper quantitative imaging system - Google Patents
Mobile terminal-based color-change diagnosis test paper quantitative imaging system Download PDFInfo
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- CN106323977A CN106323977A CN201610707152.4A CN201610707152A CN106323977A CN 106323977 A CN106323977 A CN 106323977A CN 201610707152 A CN201610707152 A CN 201610707152A CN 106323977 A CN106323977 A CN 106323977A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention discloses a mobile terminal-based color-change diagnosis test paper quantitative imaging system. The solution of an unknown solution can be accurately measured by use of colorimetric information in a relatively independent light source environment, and a cheap, fast and accurate health data measurement platform is provided; in the platform, a mobile terminal serves as a display and operation interface, and the installation process is simple and convenient; the measurement can be carried out simply by use of a mobile phone, an equipment shell, a test strip bracket and some sensing strips, for example, the measurement of glucose concentration or luteinizing hormone level in urine. The system disclosed by the invention is an integrated mobile platform based on traditional color-change diagnosis image analysis technology, and the platform can realize measurement more quantitatively than traditional color change diagnosis and can be clinically applied while integrating two functions of high-precision measurement and data visualization; and moreover, the cost is controlled in an acceptable range, and the mobile computing ability provides possibility of capturing, extracting and gathering the measured data in trend analysis from medicine respect.
Description
Technical field
The present invention relates to detection paper technical field, a kind of variable color based on mobile terminal diagnosis reagent paper is fixed
Amount imaging system.
Background technology
The variable color diagnosis of prior art has a wide range of applications in chemical concentrations is estimated.But, survey with by specialty
Chemical solution is accurately measured and is compared by amount equipment, and variable color diagnosis is measured often qualitatively, and non-quantitation.If we are only
It is desirable to show that is analyzed a conclusion qualitatively, then this method will be maximally effective in a series of reading.This
Mean that traditional variable color diagnostic method is suitable only for the autognostic carrying out pre-diagnosis at home.Need to survey because it can only provide
The limited information of examination chemical solution.
Mobile phone, as modal mobile terminal in life, is added up according to International Data Corporation (IDC) (IDC), by 2015 second
In season, smart mobile phone market, the whole world increases by 13%, reaches the shipment amount of 300,000,000 4,150 ten thousand.Increasing along with high-end smartphones demand
Adding, the mobile phone cost being today equipped with high pixel camera is more and more lower.Meanwhile, the development of mobile computing ability is to a great extent
Achieve the real-time of image procossing.Therefore, being combined in order to popular of cheap mobile technology and chemical sensitisation bar
Research topic, particularly carries out variable color diagnosis with smart mobile phone to the concentration of chemical solution.Some research and utilization smart mobile phones become
Inspection of complexion is disconnected carries out multiple sensing analysis, and some researcheres develop cl concn in mobile platform monitoring water, also have some researcheres
Then utilize mobile platform, by color sensing determination pH, oxygen content.Meanwhile, we are look for a chance by existing
The chemical solvent concentration representing health index, in smart mobile phone, is measured, as glucose solution is dense by sensor bar Technology application
Degree.
In the sensor bar of prior art, being furnished with the sensor array of 10 types, each of which has been embedded into one
Enzyme, for chemical reaction.When these arrays directly contact target solution, chemical reaction will occur, thus induce top layers
Array generation color change.Under normal/room lighting environment, the change of these colors can be observed the most easily
Arrive.After reaction completes, utilize provided with reference to figure, we can immediately read out the approximate extents of solution concentration.But, this
Individual result is difficult to be accurate to determine quantitative analysis, therefore also needs to significantly improve.
The detection of solution concentration is directly connected to medical science pre-detection, is a very important research.The most
Through there being many be in or medical worker monitors health indicator in clinic, such as the product of concentration of glucose for patient.With that
A little analytical equipments advanced in the lab are compared, and chemical sensitisation bar is the most widely used instrument.But due to its effectiveness
Restriction, it can only provide a result qualitatively.But, along with the continuous enhancing of health of people consciousness, to clinical analysis
Precise requirements also improves constantly, it means that we need a new solution that can provide quantitative data.
There is the research of many colorimetric sensings at present.Someone develops a kind of instrument so that we can at one on a large scale
Under environment, the colorimetric analysis of quantized color is carried out the measurement of pinpoint accuracy.He extracts chromatic value from the picture of mobile phone shooting,
The calibration curve of creation analysis substrate concentration rather than directly use RGB intensity, thus overcome deficiency that simple R GB analyzes it
Place, for example, it is possible to overcome the hyposensitivity problem of black color.This device is firstly the need of reference standard color reference and right
Lighting environment is proofreaded, and then measures the rgb value of mapping point interested, according to International Commission on Illumination (CIE) 1931 face
Colour space color rendering angle value carries out compareing and being quantified.Finally, complete the regression Calculation on three dimensions, and obtain measurement
Value.Provide a good mode to carry out colorimetric measurement, and take into full account the impact of lighting condition；But, this instrument
Still have several drawbacks in that its application of obstruction clinically.First, calculating nonlinear regression measured value in three dimensions is an one-tenth
The method of this costliness, it means that it needs to perform this algorithm for a long time.Secondly, this research discusses ambient light, it is believed that
By using color reference chart can make up the position because of light source, temperature, or the deficiency that outdoor lighting environment causes, because not
Between intensity measured under same environment light condition, there is linear relationship.But, this conclusion is the most universal.According to ours
Observing, unless included that time of exposure, exposure rate, light sensitivitys, even focal length etc. are all consistent at interior lighting condition, otherwise calibrating base
Standard is not necessarily linear.Finally, this research ignores the position of hand-held intelligent mobile phone camera and angle to test strip measurement
The material impact of value.
In sum, above prior art there is also and cannot realize variable color diagnosis reagent paper quantitative analysis, although can portion
Decompose certainly this technical problem, but the cost calculating nonlinear regression measured value is much more expensive, and ignore and much illuminate bar
Part and the impact of mobile terminal parameter, therefore the conclusion of its quantitative analysis is the most unreliable.
Summary of the invention
In view of this, the variable color diagnosis reagent paper that cannot realize low cost that the present invention is directed to the existence of above-mentioned prior art is quantitative
Analyze, the inaccurate technical problem of quantitative analysis conclusion, it is provided that a kind of variable color diagnosis reagent paper that can accurately realize low cost
Variable color based on the mobile terminal diagnosis reagent paper quantitative imaging system of quantitative analysis.
Variable color based on the mobile terminal diagnosis reagent paper that the technical solution of the present invention is to provide a kind of following structure is fixed
Amount imaging system, including mobile terminal and lighting control box, described mobile terminal is arranged on lighting control box, described movement
Outwardly, its photographic head direction inwardly, and arranges in described lighting control box and has completed reagent reacting at the operation interface of terminal
Sensing testing bar；
Mobile terminal described in employing completed in being opposite to lighting control box reagent reacting sensing testing bar carry out
Shooting, and extract image；Position the area-of-interest of extracted image, extract the Pixel Information of described area-of-interest；According to
Current lighting conditions, is carried out data calibration, then is estimated by precalculated linear regression model (LRM) the Pixel Information extracted
Meter reagent concentration.
As improvement, sensing testing bar is isolated by described lighting control box with ambient so that it is can only touch shifting
The light of the flash lamp of dynamic terminal；And on the inwall of lighting control box, utilize reflectorized material to form blister convex.This design energy
Enough allow test avoid the interference of ambient, simultaneously these protruding reflectance increasing Lighting control chamber interior walls, thus reach point
Dissipate the purpose of flash of light, reduce the light focusing effect as a single ray model.
As improvement, the area-of-interest of the extracted image in described location, completed by use OpenCV storehouse, institute
The OpenCV storehouse stated provides various feature detection operation, to realize the location of area-of-interest.The position of test zone is in input
Image is relatively uniform, it means that we can be carried out positioning and gathering pixel by calculating image translation simply
Information, and can automatically identify that the required region monitored is highly important.
As improvement, according to the result of described estimation reagent concentration, with the title of captured image, automatically result is presented
On mobile terminals, according to testDate, collect the data of each user, draw trendgram.The figure produced during calculating
As data visualization, improve the observability of result, and be easy to understand its variation tendency.
As improvement, described precalculated linear regression model (LRM) is estimated reagent concentration, is to carry out according to two colour gamuts
Analyzing, two described colour gamuts include the RGB color territory characterizing red, green, blue and characterize the HSB color of hue, saturation, intensity
Territory, described H represents tone, and scope is from 0-360, and it represents colour wheel angle, and S represents saturation, and it characterizes colored to ash
The distance of color, B represents brightness, and it characterizes the bright-dark degree of color.
As improvement, the use in OpenCV storehouse includes rim detection, described rim detection, uses multi-level algorithm detection figure
Various edges in Xiang, first use 5x5 Gaussian filter convolution to drop, then find the intensity gradient of image, then perform non-
Maximum suppression and hysteresis threshold, to find optimal result as edge.The principle of the program is, double check detects
The edge of image, it is seen that outer is open loop, it means that it can not connect to return formation closed loop, finds the algorithm meeting of profile
Eliminating these edges, only retain the profile closed, the program selects the profile of maximum area and displays it.
As improvement, the use in described OpenCV storehouse also includes that FAST feature detection, described FAST feature detection are
One high speed corner detection algorithm, will not introduce flagrant noise, the described FAST feature detection angle to described profile
Detect, and obtain the coordinate at each angle, make overall profile more accurate.
As improvement, described quantitative imaging system also includes that sensor bar support, described sensor bar support are provided with for passing
The locating slot that sensing strip is placed, described sensing testing bar is placed in locating slot, described sensor bar support and Lighting control
The rear side which fits of case, to completely cut off ambient.So sensing testing bar can either be positioned, be easy to again form shooting rationally
Shooting space.
As improvement, described sensing testing bar is Glucose strips or ovulation test paper.
Using above structure, the present invention compared with prior art, has the advantage that the employing present invention, relatively independent
Lighting environment under utilize colorimetric information can with the concentration of correct measurement unknown solution, and provide one cheap, the most accurate
Measurement health data platform, this platform is using mobile terminal as display and operation interface, and installation process is simple and convenient, only needs
Want mobile phone, a device housings, a test strip support and some sensor bar just can start to measure, such as, in urine
Concentration of glucose or lutropin level, the present invention is based on tradition variable color diagnostic image analysis technology integrated
Mobile platform, this platform can provide the measurement the most quantitative than tradition variable color diagnosis, and can be used for clinic, take into account height
The measurement of precision and two functions of data visualization, and by cost control at one more receptive within the scope of, mobile meter
The probability of measurement data that calculation ability also provides the seizure to trend analysis from medical angle, extracts and assembles.
Accompanying drawing explanation
Fig. 1 is the front schematic view of lighting control box；
Fig. 2 is the schematic rear view of lighting control box；
Fig. 3 is the schematic diagram of HSB；
As it can be seen, 1, mobile terminal, 2, lighting control box.
Detailed description of the invention
The invention will be further described with specific embodiment below in conjunction with the accompanying drawings, but the present invention is not restricted to these
Embodiment.
The present invention contains any replacement, amendment, equivalent method and scheme made in the spirit and scope of the present invention.For
Make the public that the present invention to be had to understand thoroughly, in present invention below preferred embodiment, concrete details is described in detail, and
The description not having these details for a person skilled in the art can also understand the present invention completely.
As it is shown in figure 1, a kind of based on mobile terminal the variable color diagnosis reagent paper quantitative imaging system of the present invention, including mobile
Terminal 1 and lighting control box 2, described mobile terminal is arranged on lighting control box, the operation interface court of described mobile terminal
Outward, inwardly, and setting has completed the sensing testing bar of reagent reacting in described lighting control box 2 in its photographic head direction；
Mobile terminal 1 described in employing completed in being opposite to lighting control box 2 reagent reacting sensing testing bar enter
Row shooting, and extract image；Position the area-of-interest of extracted image, extract the Pixel Information of described area-of-interest；Root
According to current lighting conditions, the Pixel Information extracted is carried out data calibration, then by precalculated linear regression model (LRM)
Estimate reagent concentration.
Sensing testing bar is isolated by described lighting control box with ambient so that it is can only touch the sudden strain of a muscle of mobile terminal
The light of light modulation；And on the inwall of lighting control box, utilize reflectorized material to form blister convex.This is designed to allow test exempt from
In the interference of ambient, these protruding reflectance increasing Lighting control chamber interior wall simultaneously, thus reach the mesh of dispersion flash of light
, reduce the light focusing effect as a single ray model.
The area-of-interest of the extracted image in described location, by using OpenCV storehouse to complete, described
OpenCV storehouse provides various feature detection operation, to realize the location of area-of-interest.The position of test zone is at input picture
In be relatively uniform, it means that we can be carried out positioning and gather pixel letter by calculating image translation simply
Breath, and can automatically identify that the required region monitored is highly important.
According to the result of described estimation reagent concentration, with the title of captured image, automatically result is presented on mobile whole
On end, according to testDate, collect the data of each user, draw trendgram.The view data produced during calculating can
Depending on changing, improve the observability of result, and be easy to understand its variation tendency.
Reagent concentration estimated by described precalculated linear regression model (LRM), is analyzed according to two colour gamuts, institute
Two colour gamuts stated include the RGB color territory characterizing red, green, blue and characterize the HSB colour gamut of hue, saturation, intensity, described H
Representing tone, scope is from 0-360, and it represents colour wheel angle, and S represents saturation, and it characterizes the colored distance to Lycoperdon polymorphum Vitt, B
Representing brightness, it characterizes the bright-dark degree of color.
The use in OpenCV storehouse includes rim detection, described rim detection, and use in multi-level algorithm detection image is each
Plant edge, first use 5x5 Gaussian filter convolution to drop, then find the intensity gradient of image, then perform non-maximum and press down
System and hysteresis threshold, to find optimal result as edge.The principle of the program is, the limit of double check detection image
Edge, it is seen that outer is open loop, it means that it can not connect to return formation closed loop, the algorithm finding profile can eliminate these
Edge, only retains the profile closed, and the program selects the profile of maximum area and displays it.
The use in described OpenCV storehouse also includes that FAST feature detection, described FAST feature detection are a high speed angle
Fall detection algorithm, will not introduce flagrant noise, and the angle of described profile is detected by described FAST feature detection, and
Obtain the coordinate at each angle, make overall profile more accurate.
Described quantitative imaging system also includes that sensor bar support, described sensor bar support are provided with and puts for sensing testing bar
The locating slot put, described sensing testing bar is placed in locating slot, and described sensor bar support is joined after lighting control box
Close, to completely cut off ambient.So sensing testing bar can either be positioned, be easy to again form shooting and reasonably shoot sky
Between.
Described sensing testing bar is Glucose strips or ovulation test paper.
Below only preferred embodiment of the present invention is described, but is not to be construed as limitations on claims.This
Invention is not only limited to above example, and its concrete structure allows to change.In a word, all guarantors in independent claims of the present invention
The various changes made in the range of protecting are the most within the scope of the present invention.
Claims (9)
1. variable color based on a mobile terminal diagnosis reagent paper quantitative imaging system, including mobile terminal and lighting control box, its
Be characterised by: described mobile terminal is arranged on lighting control box, the operation interface of described mobile terminal outwardly, its photographic head
Direction inwardly, and arranges the sensing testing bar having completed reagent reacting in described lighting control box；
Mobile terminal described in employing completed in being opposite to lighting control box reagent reacting sensing testing bar shoot,
And extract image；Position the area-of-interest of extracted image, extract the Pixel Information of described area-of-interest；Shine according to current
Bright condition, carries out data calibration, then estimates reagent by precalculated linear regression model (LRM) the Pixel Information extracted
Concentration.
Variable color based on mobile terminal the most according to claim 1 diagnosis reagent paper quantitative imaging system, it is characterised in that: institute
Sensing testing bar is isolated by the lighting control box stated with ambient so that it is can only touch the light of the flash lamp of mobile terminal
Line；And on the inwall of lighting control box, utilize reflectorized material to form blister convex.
Variable color based on mobile terminal the most according to claim 1 and 2 diagnosis reagent paper quantitative imaging system, its feature exists
In: the area-of-interest of the extracted image in described location, by using OpenCV storehouse to complete, described OpenCV storehouse carries
For various feature detection operation, to realize the location of area-of-interest.
Variable color based on mobile terminal the most according to claim 2 diagnosis reagent paper quantitative imaging system, it is characterised in that: root
According to the result of described estimation reagent concentration, with the title of captured image, automatically result is presented on mobile terminals, according to survey
On the examination date, collect the data of each user, draw trendgram.
Variable color based on mobile terminal the most according to claim 1 diagnosis reagent paper quantitative imaging system, it is characterised in that: institute
Reagent concentration estimated by the precalculated linear regression model (LRM) stated, and is analyzed according to two colour gamuts, two described colors
Territory includes the RGB color territory characterizing red, green, blue and characterizes the HSB colour gamut of hue, saturation, intensity, and described H represents tone, model
Enclosing from 0-360, it represents colour wheel angle, and S represents saturation, and it characterizes the colored distance to Lycoperdon polymorphum Vitt, and B represents brightness, it
Characterize the bright-dark degree of color.
Variable color based on mobile terminal the most according to claim 3 diagnosis reagent paper quantitative imaging system, it is characterised in that:
The use in OpenCV storehouse includes rim detection, described rim detection, uses the various edges in multi-level algorithm detection image, first
Use 5x5 Gaussian filter convolution to drop, then find the intensity gradient of image, then perform the suppression of non-maximum and delayed threshold
Value, to find optimal result as edge.The principle of the program is, the edge of double check detection image, it is seen that
Outer is open loop, it means that it can not connect to return formation closed loop, and the algorithm finding profile can eliminate these edges, only protects
Staying the profile of closing, the program selects the profile of maximum area and displays it.
Variable color based on mobile terminal the most according to claim 6 diagnosis reagent paper quantitative imaging system, it is characterised in that: institute
The use in the OpenCV storehouse stated also includes that FAST feature detection, described FAST feature detection are that a high speed corner detection is calculated
Method, will not introduce flagrant noise, and the angle of described profile is detected, and obtained each by described FAST feature detection
The coordinate at angle, makes overall profile more accurate.
Variable color based on mobile terminal the most according to claim 1 diagnosis reagent paper quantitative imaging system, it is characterised in that: institute
The quantitative imaging system stated also includes that sensor bar support, described sensor bar support are provided with the location placed for sensing testing bar
Groove, described sensing testing bar is placed in locating slot, and described sensor bar support and the rear side which fits of lighting control box, with isolation
Ambient.
Variable color based on mobile terminal the most according to claim 8 diagnosis reagent paper quantitative imaging system, it is characterised in that: institute
The sensing testing bar stated is Glucose strips or ovulation test paper.
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| CN109187946B (en) * | 2018-10-22 | 2022-02-15 | 湖南达道生物工程有限公司 | Foldable colloidal gold/immunofluorescence test device and test method |
| CN109596606A (en) * | 2018-12-12 | 2019-04-09 | 华南理工大学 | A kind of ascorbic acid colorimetric detection method based on degradability nano enzyme |
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