CN107121542A - A kind of immunity-chromatography test strip quantitative detection system and method based on mobile phone - Google Patents
A kind of immunity-chromatography test strip quantitative detection system and method based on mobile phone Download PDFInfo
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- CN107121542A CN107121542A CN201710293313.4A CN201710293313A CN107121542A CN 107121542 A CN107121542 A CN 107121542A CN 201710293313 A CN201710293313 A CN 201710293313A CN 107121542 A CN107121542 A CN 107121542A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04M—TELEPHONIC COMMUNICATION
- H04M1/00—Substation equipment, e.g. for use by subscribers
- H04M1/72—Mobile telephones; Cordless telephones, i.e. devices for establishing wireless links to base stations without route selection
- H04M1/724—User interfaces specially adapted for cordless or mobile telephones
- H04M1/72403—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04M—TELEPHONIC COMMUNICATION
- H04M1/00—Substation equipment, e.g. for use by subscribers
- H04M1/72—Mobile telephones; Cordless telephones, i.e. devices for establishing wireless links to base stations without route selection
- H04M1/724—User interfaces specially adapted for cordless or mobile telephones
- H04M1/72403—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality
- H04M1/72406—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality by software upgrading or downloading
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04M—TELEPHONIC COMMUNICATION
- H04M1/00—Substation equipment, e.g. for use by subscribers
- H04M1/72—Mobile telephones; Cordless telephones, i.e. devices for establishing wireless links to base stations without route selection
- H04M1/724—User interfaces specially adapted for cordless or mobile telephones
- H04M1/72403—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality
- H04M1/7243—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality with interactive means for internal management of messages
- H04M1/72439—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality with interactive means for internal management of messages for image or video messaging
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Abstract
The invention discloses a kind of immunity-chromatography test strip quantitative detection system based on mobile phone and method, system includes image acquisition hardware device and mobile phone application module;User obtains strip image by image acquisition hardware module and mobile phone camera, the processing of strip image and the calculating of strip concentration value are carried out using mobile phone application module afterwards, and use the upload in application module and download function, the information interchange of patient, relatives and medical personnel tripartite are realized, medical advice and guidance are obtained in time.The present invention has the advantages that:1) accuracy and validity of quantitative detection be ensure that;2) it patient from far-off regions can conveniently see a doctor, realize tele-medicine, optimization medical treatment configuration;3) working doctor amount is mitigated, by the automatically derived concentration of software, without artificial chemical examination;4) medical treatment cost is reduced.
Description
Technical field
The present invention relates to immunity-chromatography test strip quantitative test technique field, particularly a kind of immunity-chromatography test based on mobile phone
Strip quantitative detection system and method.
Background technology
Trend of the immunochormatography due to meeting " bedside detection " that modern medicine is advocated, with specificity
By force, operating method is simple, efficiency high, instruct without professional person, the features such as sensitivity is high and as a kind of the most frequently used effluent
Immunochromatography quick determination method.Immunity-chromatography test strip determination techniques were quickly grown in recent years, and application expands day by day, almost
All aspects of immunology diagnosis detection are covered, but it can only provide qualitative results, it is difficult to the market demand is met, thus is occurred
A variety of quantitative detecting methods for immunity-chromatography test strip.
Among numerous methods, the concern that the strip quantitative detecting method based on image is subject at most, has triggered Research Center
And enterprise research boom.The collection of strip image is general by special CCD or COMS imaging sensors, then using individual
People's computer or the processor of costliness carry out the processing of next step, and obtained result does not also carry out the utilization of next step, if
Standby also to lack portability and practicality, function is single.
At present, mobile phone has become the daily necessities of people, can directly obtain strip figure by cell-phone camera function
As information, the processing of strip image is carried out using the powerful disposal ability of mobile phone.On the other hand, online medical treatment cooperation is more and more wider
It is accepted by the public, patient can be exchanged without going to a doctor on the spot with doctor by internet, this " is seen alleviating to a certain degree
It is sick difficult " the problem of, the resident for facilitating remote districts obtains medical advice and guidance in time, it is possible to utilize the communication of mobile phone
Function, realizes the timely exchange and data sharing of testing result.But matching somebody with somebody between the stability and image processing algorithm of mobile phone imaging
Conjunction is still difficult point, therefore the present invention proposes a kind of general immunity-chromatography test strip quantitative detection system based on mobile phone.
The content of the invention
That invents aims to overcome that the deficiency of prior art is quantitatively examined there is provided a kind of immunity-chromatography test strip based on mobile phone
Examining system and method, not only contribute to improve mobile phone imaging definition and stability, and can be suitably used for each smart mobile phone,
Strip image according to obtaining can calculate concentration value.Testing result can be uploaded and notify relatives and medical care people by user
Member, after medical personnel's diagnosis terminates, user and relatives can also download and read the medical advice and refer to that medical personnel provide
Lead, the present invention is easily operated, and applied widely, using effect is good.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of immunity-chromatography test strip quantitative detection system based on mobile phone, including:Mobile phone, elastic fixing clip, rail plate,
Flight data recorder and strip;The elastic fixing clip is connected with the mobile phone to be carried out for the position of the width according to mobile phone and camera
It is mobile;The rail plate is fixedly connected with the elastic fixing clip;The flight data recorder is slidably disposed within the slip and led
On rail;The mobile phone includes camera and application module, and the application module includes the figure that image taking is carried out to the strip
The graphics processing unit that is handled as shooting unit, to the image of shooting, concentration value calculating is carried out to processing result image
Concentration value computing unit, the testing result uploading unit that concentration value is transmitted and medical treatment guidance opinion download unit;It is described
Strip is fixed adjustably to the flight data recorder inner top;The flight data recorder bottom is provided with an aperture;The application module is carried out
During image taking, the detection window of the camera, aperture and strip is in same straight line.
It is preferred that, the flight data recorder is ferrous material.
It is preferred that, slot of the flight data recorder inner top provided with one side opening inserts for the strip, the slot
Bottom is provided with opening.
It is preferred that, the quantitative detection system also includes a magnetic strip fixed block;The magnetic strip fixed block is placed
It is used to the strip being fixed to the irony flight data recorder in strip bottom.
It is preferred that, the mobile phone also includes a convex mirror;The convex mirror is close to the camera and focused for mobile phone.
It is preferred that, it is placed with two green light LED lamps above the flight data recorder inner bottom part.
It is preferred that, scattering sheet is placed with respectively above the two green light LEDs lamp.
Application module of the present invention mainly realizes that image taking, image procossing, concentration value are calculated and testing result is uploaded
With medical treatment guidance opinion download function, including image capturing unit, graphics processing unit, concentration value computing unit, testing result
Uploading unit and medical treatment guidance opinion download unit.
Described image shooting unit can analyze current image quality quality, when imaging meets accessible regulation, carry
Show that user can shoot;If being unsatisfactory for processing requirement, it is impossible to shooting image.
Described image processing unit includes image gray processing, noise reduction deblurring, extracts detection window and extracts detection line
And nature controlling line.
Described image gray processing includes the strip image shot carrying out gray processing processing, makes the value of each pixel
In 0 to 255 interval.
The noise reduction deblurring includes removing image using weighted mean filter and medium filtering for strip feature of image
In Gaussian noise and salt-pepper noise.
The extraction detection window includes:First by the detection window edge of edge detection operator determination substantially;It is right again
The pixel grey scale of specific region carries out accumulation calculating, obtains the boundary coordinate of detection window, is carried according to boundary coordinate from image
Take out detection window.
The extraction detection line and nature controlling line include being directed to detection window and strip feature of image, use adaptive threshold
Fuzzy saltus step cell neural network algorithm, extracts the detection line and nature controlling line of strip.
Detection line and nature controlling line information are quantified, passed through according to langbobier law by the concentration value computing unit
The working curve of characteristic value and concentration after quantization draws the concentration levels of strip to be measured.
Testing result uploading unit and medical treatment guidance the opinion download unit can realize the message interconnection sum between doctors and patients
It is shared according to base resource.Its detection information can be uploaded to database by patient after login account, facilitate medical personnel and relatives
Solve patient profiles.Medical personnel for sufferer testing result after medical diagnosis is made, by medical treatment guidance opinion and detailed inspection
Observe and predict announcement and be uploaded to database.Sufferer and relatives can receive the information push for coming from application module afterwards, notify its timely
Medical treatment guidance opinion and examining report are downloaded, to carry out the diagnosis and treatment of next step.
A kind of immunity-chromatography test strip quantitative detecting method based on mobile phone, including:
Step a, opens mobile phone camera, elastic fixing clip is fixed on into phone housing, moving left and right elastic fixing clip makes
The aperture for obtaining camera and flight data recorder is located at same plummet face;Flight data recorder is slided into the aperture until flight data recorder along rail plate
Camera is directed at, is fixed with screw;Detection strip is then inserted into, sliding strip makes its detection window just to camera, profit
Strip is fixed in flight data recorder with magnetic strip fixed block and avoids rocking;Two green light LED lamps are opened, regulation brightness is until lamp
Light is soft to be evenly distributed on detection window;
Step b, mobile phone application module, which is received, starts request, if focusing failure, shows " right in the mobile phone screen upper right corner
Jiao's failure, please focuses again ";If focused successfully, detection window imaging clearly is then shown " now in the mobile phone screen upper right corner
Can be shot ", receive after shooting request, photographed clearly pending image;
Step c, is received after determination request, and mobile phone application module carries out graphics processing unit and concentration value is calculated, including:
Gray processing is carried out using the pending image of shooting as input;The drop of image is carried out using weighted mean filter and medium filtering
Make an uproar;Detection window is extracted using the strategy of edge detection operator and specific region pixel grey scale accumulation calculating;Use adaptive thresholding
The fuzzy saltus step cell neural network algorithm of value extracts detection line and nature controlling line;Detection line and nature controlling line information are measured
Change, the concentration levels of strip to be measured are drawn by the characteristic value and the working curve of concentration after quantization;
Step d, mobile phone application module shows the existing concentration of strip to be measured in jump page, and reception is checked oneself report and asked
Ask, uploading detection result is asked to relatives and uploading detection result is asked to doctor;
Step f, mobile phone application module receives the medical diagnosis result and medical treatment guidance opinion of physician feedback, notifies detection knot
Fruit uploader downloads medical diagnosis result and medical treatment guidance opinion.
The beneficial effects of the invention are as follows:
(1) accuracy and validity of quantitative detection be ensure that;
(2) it patient from far-off regions can conveniently see a doctor, realize tele-medicine, optimization medical treatment configuration;
(3) working doctor amount is mitigated, by the automatically derived concentration of software, without artificial chemical examination;
(4) medical treatment cost is reduced.
The present invention is described in further detail below in conjunction with drawings and Examples;But the present invention's is a kind of based on mobile phone
Immunity-chromatography test strip quantitative detection system and method are not limited to embodiment.
Brief description of the drawings
Fig. 1 is the structural representation of present system;
Fig. 2 is the flight data recorder internal structure schematic diagram of present system;
Fig. 3 is mobile phone application module page Fig. 1 of the present invention;
Fig. 4 is mobile phone application module page Fig. 2 of the present invention;
Fig. 5 is the workflow diagram of the present invention.
Embodiment
A kind of immunity-chromatography test strip quantitative detection system based on mobile phone of the present invention, including image acquisition hardware device and hand
Machine application module, the mobile phone is smart mobile phone, includes camera and convex mirror, and can realize mobile phone by software programming
The function of application module.
Referring to shown in Fig. 1 and Fig. 2, described image acquisition hardware device includes mobile phone 101, elastic fixing clip 102, slides and lead
Rail 103 and flight data recorder 104.The rail plate 103 is fixedly connected with elastic fixing clip 102;The elastic fixing clip 102 can root
Moved according to the width of mobile phone 101 and the position of camera;The flight data recorder 104 can slidably reciprocate directly in rail plate 103
Aperture 205 to flight data recorder is directed at the camera position of mobile phone 101.Be close to the camera of mobile phone 101 is a piece of convex mirror
206, focused on for mobile phone 101.
Described image acquisition hardware device also includes magnetic strip fixed block 201, conventional strip 202, scattering sheet 203 and green
Light LED 204.The flight data recorder 104 is ferrous material, and slot of its inner top provided with one side opening is for the strip
202 insertions, the slot bottom makes its detection window during the insertion of strip 202 not be blocked provided with opening.The magnetic examination
Bar fixed block 201 is placed on the bottom of the strip 202, because flight data recorder 104 is ferrous material, the magnetic strip fixed block
Strip 202 can be fixed on flight data recorder inner top by 201, it is to avoid it is rocked.The green light LED lamp 204 includes two, and it is placed
Above the inner bottom part of flight data recorder 104, lamplight brightness can be adjusted according to external environment condition, and (LED is by being attached to button below
Battery is powered).The scattering sheet 203 includes two, is individually positioned in the top of two green light LED lamp 204, two symmetrical scattering sheets
203 enable light to be evenly distributed in strip 202.Because of the position difference of different mobile phone cameras, it can adjust
The depth of strip 202 is inserted, the mobile flight data recorder 104 on guide rail 103 so that camera, aperture and strip detection window are in
Same straight line, the image acquisition hardware device can obtain the image of clear high resolution.
The mobile phone application module includes image capturing unit, graphics processing unit, concentration value computing unit, testing result
Uploading unit and medical treatment guidance opinion download unit.
Described image shooting unit can analyze current image quality quality, when imaging meets accessible regulation, carry
Show that user can shoot;If being unsatisfactory for processing requirement, it is impossible to shooting image.
Described image processing unit includes image gray processing, noise reduction deblurring, extracts detection window and extracts detection line
And nature controlling line.
Described image gray processing includes the strip image shot carrying out gray processing processing, makes the value of each pixel
In 0 to 255 interval.
The noise reduction deblurring includes removing image using weighted mean filter and medium filtering for strip feature of image
In Gaussian noise and salt-pepper noise.
The extraction detection window includes:First by the detection window edge of edge detection operator determination substantially;It is right again
The pixel grey scale of specific region carries out accumulation calculating, obtains the boundary coordinate of detection window, is carried according to boundary coordinate from image
Take out detection window.
The extraction detection line and nature controlling line include being directed to detection window and strip feature of image, use adaptive threshold
Fuzzy saltus step cell neural network algorithm, extracts the detection line and nature controlling line of strip.
Detection line and nature controlling line information are quantified, passed through according to langbobier law by the concentration value computing unit
The working curve of characteristic value and concentration after quantization draws the concentration levels of strip to be measured.
Testing result uploading unit and medical treatment guidance the opinion download unit can realize the message interconnection sum between doctors and patients
It is shared according to base resource.Its detection information can be uploaded to database by patient after login account, facilitate medical personnel and relatives
Solve patient profiles.Medical personnel for sufferer testing result after medical diagnosis is made, by medical treatment guidance opinion and detailed inspection
Observe and predict announcement and be uploaded to database.Sufferer and relatives can receive the information push for coming from application module afterwards, notify its timely
Medical treatment guidance opinion and examining report are downloaded, to carry out the diagnosis and treatment of next step.
Mobile phone application module page Fig. 1 of the present invention is illustrated in figure 3, when the prompting 303 for occurring to shoot now,
Click on and determine 302, application module starts identification image and carries out image procossing, calculates concentration value;Can be with to abandon this time shooting
Cancellation 301 is clicked on to adjust again.
Mobile phone application module page Fig. 2 of the present invention is illustrated in figure 4, existing concentration is shown in application module upper right side
401, patient, which can click on, checks oneself report 402, and this application module is automatically connected in hospital database, can be found according to concentration and oneself
Oneself related indication diagnosis report, greatly facilitates patient to understand the state of an illness, without waiting in line, for long-term with same disease
Patient, such as diabetic, learn whether the state of an illness takes a turn for the better without going to hospital to register, and need to only be in can be learnt using the present invention
Concentration, own situation is understood by contrast;On the other hand, if itself can not accurately analysing patient's condition, or have Dyslexia
Personage, can select to send to doctor 404 or send to relatives 403, doctor is according to medical experience and hospital database information point
Conditions of patients is analysed, and related advisory can be provided, allows patient to take medicine and the intake that is careful in one's diet.Greatly facilitate remote mountain
The resident in area sees a doctor, optimization medical treatment configuration.
As shown in figure 5, a kind of immunity-chromatography test strip quantitative detecting method based on mobile phone, comprises the following steps:
Mobile phone camera is opened, elastic force geometrical clamp is fixed on phone housing, elastic force geometrical clamp is moved left and right and causes shooting
The aperture of head and flight data recorder is located at same plummet face;Flight data recorder is allowed to be slided along rail plate until the aperture alignment of flight data recorder is taken the photograph
As head, it is fixed in screw on rail plate and avoids rocking;Detection strip is then inserted into, sliding strip makes detection window just
To camera, strip will be chromatographed using magnetic strip fixed block (such as magnet) it will be fixed in flight data recorder and avoid rocking, will finally image
Head, aperture and detection window should be in line;Two LEDs are opened, regulation brightness is uniformly distributed in detection until light is soft
Window;
Mobile phone application module is opened, if focusing failure, occur in the mobile phone screen upper right corner " focusing failure, please again
Focusing " printed words;There is " can be shot now " word to defocused, detection window imaging clearly, the mobile phone screen upper right corner in success
Sample, presses shooting, you can obtain clearly pending image;
Click on and determine, application module brings into operation image processing function, including:It regard the pending image of shooting as input
Carry out gray processing;The noise reduction of image is carried out using weighted mean filter and medium filtering;Use edge detection operator and given zone
The strategy of domain pixel grey scale accumulation calculating extracts detection window;Use the fuzzy saltus step cell neural network algorithm of adaptive threshold
Extract detection line and nature controlling line;Detection line and nature controlling line information are quantified, pass through the characteristic value after quantization and concentration
Working curve draws the concentration levels of strip to be measured;
Mobile phone jump page, the upper right side of screen shows existing concentration, and to check oneself report button, (patient can basis for centre
The information that various concentrations are represented in the database of hospital is contrasted with oneself existing concentration), it can also select to be sent to relatives,
The two buttons of doctor, their possible possibly can not accurately understand some professional terms for the elderly, can select
Relatives are sent to, are sent by it to doctor.
Doctor receive information push after, according to medical experience and concentration data storehouse draw examining report feed back to patient and
In relatives' hand, if there is something special, patient can also be contacted according to the patient's number provided in database.
Above-described embodiment is only used for further illustrating that a kind of immunity-chromatography test strip based on mobile phone of the present invention is quantitatively detected
System and method, but the invention is not limited in embodiment, every technical spirit according to the present invention is made to above example
Any simple modification, equivalent variations and modification, each fall within the protection domain of technical solution of the present invention.
Claims (9)
1. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone, it is characterised in that including:Mobile phone (101), elasticity are solid
Clamp (102), rail plate (103), flight data recorder (104) and strip (202);The elastic fixing clip (102) and the mobile phone
(101) it is connected for being moved according to the width of mobile phone (101) and the position of camera;The rail plate (103) and institute
Elastic fixing clip (102) is stated to be fixedly connected;The flight data recorder (104) is slidably disposed within the rail plate (103);Institute
Stating mobile phone (101) includes camera and application module, and the application module includes carrying out image taking to the strip (202)
Graphics processing unit that image capturing unit, the image to shooting are handled, concentration value calculating is carried out to processing result image
Concentration value computing unit, testing result uploading unit that concentration value is transmitted and medical treatment guidance opinion download unit;Institute
State strip (202) and be fixed adjustably to the flight data recorder (104) inner top;Flight data recorder (104) bottom is provided with an aperture
(205);When the application module carries out image taking, the detection window of the camera, aperture (205) and strip is in same
Straight line.
2. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 1, it is characterised in that:Institute
Flight data recorder (104) is stated for ferrous material.
3. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 2, it is characterised in that:Institute
Slot of flight data recorder (104) inner top provided with one side opening is stated so that the strip (202) is inserted, the slot bottom is provided with
Opening.
4. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 3, it is characterised in that:Institute
Stating quantitative detection system also includes a magnetic strip fixed block (201);The magnetic strip fixed block (201) is placed on strip
(202) bottom is used to the strip (202) being fixed to the irony flight data recorder (104).
5. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 4, it is characterised in that:Institute
Magnetic strip fixed block (201) is stated for magnet.
6. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 1, it is characterised in that:Institute
Stating mobile phone also includes a convex mirror (206);The convex mirror (206) is close to the camera and focused for mobile phone.
7. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 1, it is characterised in that:Institute
State and be placed with two green light LED lamps (204) above flight data recorder (104) inner bottom part.
8. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 7, it is characterised in that:Institute
State and be placed with scattering sheet (203) respectively above two green light LED lamps (204).
9. a kind of immunity-chromatography test strip quantitative detecting method based on mobile phone, it is characterised in that including:
Step a, opens mobile phone camera, elastic fixing clip is fixed on into phone housing, elastic fixing clip is moved left and right so that taking the photograph
As the aperture of head and flight data recorder is located at same plummet face;Flight data recorder is slided along rail plate until the aperture of flight data recorder is aligned
Camera, is fixed with screw;Detection strip is then inserted into, sliding strip makes its detection window just to camera, utilize magnetic
Strip is fixed in flight data recorder by property strip fixed block to be avoided rocking;Two green light LED lamps, regulation brightness are opened until light is soft
Be evenly distributed on detection window;
Step b, mobile phone application module, which is received, starts request, if focusing failure, shows that " focusing is lost in the mobile phone screen upper right corner
Lose, please focus again ";If focused successfully, detection window imaging clearly, then being shown in the mobile phone screen upper right corner " now can be with
Shot ", receive after shooting request, photographed clearly pending image;
Step c, is received after determination request, and mobile phone application module carries out graphics processing unit and concentration value is calculated, including:It will clap
The pending image taken the photograph carries out gray processing as input;The noise reduction of image is carried out using weighted mean filter and medium filtering;Make
Detection window is extracted with the strategy of edge detection operator and specific region pixel grey scale accumulation calculating;Use the mould of adaptive threshold
Paste saltus step cell neural network algorithm extracts detection line and nature controlling line;Detection line and nature controlling line information are quantified, passed through
The working curve of characteristic value and concentration after quantization draws the concentration levels of strip to be measured;
Step d, mobile phone application module shows the existing concentration of strip to be measured in jump page, and receive check oneself report request, on
Testing result is passed to ask to doctor to relatives' request and uploading detection result;
Step f, mobile phone application module receives the medical diagnosis result and medical treatment guidance opinion of physician feedback, notifies in testing result
Biography person downloads medical diagnosis result and medical treatment guidance opinion.
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