CN107121542B - A kind of immunity-chromatography test strip quantitative detection system and method based on mobile phone - Google Patents
A kind of immunity-chromatography test strip quantitative detection system and method based on mobile phone Download PDFInfo
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- CN107121542B CN107121542B CN201710293313.4A CN201710293313A CN107121542B CN 107121542 B CN107121542 B CN 107121542B CN 201710293313 A CN201710293313 A CN 201710293313A CN 107121542 B CN107121542 B CN 107121542B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04M—TELEPHONIC COMMUNICATION
- H04M1/00—Substation equipment, e.g. for use by subscribers
- H04M1/72—Mobile telephones; Cordless telephones, i.e. devices for establishing wireless links to base stations without route selection
- H04M1/724—User interfaces specially adapted for cordless or mobile telephones
- H04M1/72403—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04M—TELEPHONIC COMMUNICATION
- H04M1/00—Substation equipment, e.g. for use by subscribers
- H04M1/72—Mobile telephones; Cordless telephones, i.e. devices for establishing wireless links to base stations without route selection
- H04M1/724—User interfaces specially adapted for cordless or mobile telephones
- H04M1/72403—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality
- H04M1/72406—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality by software upgrading or downloading
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04M—TELEPHONIC COMMUNICATION
- H04M1/00—Substation equipment, e.g. for use by subscribers
- H04M1/72—Mobile telephones; Cordless telephones, i.e. devices for establishing wireless links to base stations without route selection
- H04M1/724—User interfaces specially adapted for cordless or mobile telephones
- H04M1/72403—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality
- H04M1/7243—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality with interactive means for internal management of messages
- H04M1/72439—User interfaces specially adapted for cordless or mobile telephones with means for local support of applications that increase the functionality with interactive means for internal management of messages for image or video messaging
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Abstract
The invention discloses a kind of immunity-chromatography test strip quantitative detection system and method based on mobile phone, system includes image acquisition hardware device and mobile phone application module；User obtains strip image by image acquisition hardware module and mobile phone camera, the calculating of the processing and strip concentration value of strip image is carried out using mobile phone application module later, and use the upload in application module and download function, the information interchange for realizing patient, relatives and medical staff tripartite, obtains medical advice and guidance in time.The present invention has the advantages that：1) it can ensure the accuracy and validity of quantitative detection；2) patient from far-off regions can be facilitated to see a doctor, realize tele-medicine, optimize medical treatment configuration；3) mitigate working doctor amount, by the automatically derived concentration of software, without artificial chemical examination；4) medical treatment cost is reduced.
Description
Technical field
The present invention relates to immunity-chromatography test strip quantitative test technique field, especially a kind of immunity-chromatography test based on mobile phone
Strip quantitative detection system and method.
Background technology
Trend of the immunochormatography due to meeting " bedside detection " that modern medicine is advocated has specificity
By force, operating method it is simple, it is efficient, instructed without professional person, become a kind of most common effluent the features such as high sensitivity
Immunochromatography rapid detection method.Immunity-chromatography test strip determination techniques were quickly grown in recent years, and application range is growing, almost
All aspects of immunology diagnosis detection are covered, but it can only provide qualitative results, it is difficult to meet the market demand, thus occur
A variety of quantitative detecting methods for immunity-chromatography test strip.
Among numerous methods, the concern that the strip quantitative detecting method based on image is subject to is most, has caused Research Center
And enterprise research boom.Then the acquisition of strip image uses a generally by special CCD or COMS imaging sensors
People's computer or costliness processor carry out next step processing, obtained result also without progress next step utilization, if
It is standby also to lack portability and practicability, it has a single function.
Currently, mobile phone has become people’s lives necessity, strip figure can be directly acquired by cell-phone camera function
As information, the processing of strip image is carried out using the powerful processing capacity of mobile phone.On the other hand, online medical treatment cooperation is more and more wider
It is accepted by the public, patient can be exchanged by internet without going to a doctor on the spot with doctor, this " sees alleviating to a certain degree
It is sick difficult " the problem of, the resident for facilitating remote districts obtains medical advice and guidance in time, it is possible to utilize the communication of mobile phone
Function realizes the timely exchange and data sharing of testing result.But matching between the stability and image processing algorithm of mobile phone imaging
Conjunction is still difficult point, therefore the present invention proposes a kind of general immunity-chromatography test strip quantitative detection system based on mobile phone.
Invention content
The deficiency for aiming to overcome that the prior art of invention provides a kind of immunity-chromatography test strip based on mobile phone and quantitatively examines
Examining system and method not only contribute to improve mobile phone imaging definition and stability, and can be suitably used for each smart mobile phone,
Concentration value can be calculated according to obtained strip image.Testing result can be uploaded and notify relatives and medical care people by user
Member, after medical staff diagnoses, user and relatives can also download and read the medical advice and refer to that medical staff provides
It leads, the present invention is easily operated, and applied widely, using effect is good.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of immunity-chromatography test strip quantitative detection system based on mobile phone, including：Mobile phone, elastic fixing clip, rail plate,
Flight data recorder and strip；The elastic fixing clip is connected with the mobile phone for being carried out according to the width of mobile phone and the position of camera
It is mobile；The rail plate is fixedly connected with the elastic fixing clip；The flight data recorder is slidably disposed within the sliding and leads
On rail；The mobile phone includes camera and application module, and the application module includes the figure that image taking is carried out to the strip
The image processing unit that is handled as shooting unit, to the image of shooting carries out concentration value calculating to processing result image
Concentration value computing unit, the testing result uploading unit that concentration value is sent and medical treatment guidance opinion download unit；It is described
Strip is fixed adjustably to the flight data recorder inner top；The flight data recorder bottom is equipped with an aperture；The application module carries out
When image taking, the detection window of the camera, aperture and strip is in same straight line.
Preferably, the flight data recorder is ferrous material.
Preferably, the flight data recorder inner top is equipped with the slot of one side opening and is inserted into for the strip, the slot
Bottom is equipped with opening.
Preferably, the quantitative detection system further includes a magnetic strip fixed block；The magnetism strip fixed block is placed
It is used to the strip being fixed to the irony flight data recorder in strip bottom.
Preferably, the mobile phone further includes a convex mirror；The convex mirror is close to the camera and focuses for mobile phone.
Preferably, two green light LED lamps are placed with above bottom in the flight data recorder.
Preferably, it is placed with scattering sheet respectively above the two green light LEDs lamp.
Application module of the present invention mainly realizes that image taking, image procossing, concentration value calculate and testing result uploads
With medical treatment guidance opinion download function, including image capturing unit, image processing unit, concentration value computing unit, testing result
Uploading unit and medical treatment guidance opinion download unit.
Described image shooting unit can analyze current image quality quality, when imaging meets accessible regulation, carry
Show that user can shoot；If being unsatisfactory for processing requirement, image can not be shot.
Described image processing unit includes image gray processing, noise reduction deblurring, extraction detection window and extraction detection line
And nature controlling line.
Described image gray processing includes that the strip image that will have been shot carries out gray processing processing, makes the value of each pixel
In 0 to 255 section.
The noise reduction deblurring includes removing image using weighted mean filter and medium filtering for strip feature of image
In Gaussian noise and salt-pepper noise.
The extraction detection window includes：First rough detection window edge is determined using edge detection operator；It is right again
The pixel grey scale of specific region carries out accumulation calculating, obtains the boundary coordinate of detection window, is carried from image according to boundary coordinate
Take out detection window.
The extraction detection line and nature controlling line include being directed to detection window and strip feature of image, use adaptive threshold
Fuzzy saltus step cell neural network algorithm, extracts the detection line and nature controlling line of strip.
Detection line and nature controlling line information are quantified, are passed through according to langbobier law by the concentration value computing unit
The working curve of characteristic value and concentration after quantization obtains the concentration levels of strip to be measured.
The testing result uploading unit and medical treatment guidance opinion download unit can realize the message interconnection sum number between doctors and patients
It is shared according to base resource.Its detection information can be uploaded to database by patient after login account, facilitate medical staff and relatives
Solve patient profiles.Medical staff for sufferer testing result after making medical diagnosis, by medical treatment guidance opinion and detailed inspection
It observes and predicts announcement and is uploaded to database.Sufferer and relatives will receive the information push for coming from application module later, notify it in time
Medical treatment guidance opinion and examining report are downloaded, to carry out the diagnosis and treatment of next step.
A kind of immunity-chromatography test strip quantitative detecting method based on mobile phone, including：
Step a opens mobile phone camera, elastic fixing clip is fixed on phone housing, moving left and right elastic fixing clip makes
The aperture for obtaining camera and flight data recorder is located at same plummet face；By flight data recorder along rail plate sliding until the aperture of flight data recorder
It is directed at camera, is fixed with screw；It is then inserted into detection strip, sliding strip makes its detection window face camera, profit
Strip is fixed in flight data recorder with magnetic strip fixed block and avoids shaking；Two green light LED lamps are opened, adjust brightness until lamp
Light is soft to be evenly distributed on detection window；
Step b, mobile phone application module, which receives, starts request, if focusing failure, it is " right to be shown in the mobile phone screen upper right corner
Coke failure, please focuses again "；If focused successfully, detection window imaging clearly is then shown " now in the mobile phone screen upper right corner
Can be shot ", after receiving shooting request, taken clearly pending image；
Step c is received after determining request, and mobile phone application module carries out image processing unit and concentration value calculates, including：
Gray processing is carried out using the pending image of shooting as input；The drop of image is carried out using weighted mean filter and medium filtering
It makes an uproar；Use the strategy extraction detection window of edge detection operator and specific region pixel grey scale accumulation calculating；Use adaptive thresholding
The fuzzy saltus step cell neural network algorithm of value extracts detection line and nature controlling line；By detection line and the nature controlling line information amount of progress
Change, the concentration levels of strip to be measured are obtained by the working curve of characteristic value and concentration after quantization；
Step d, mobile phone application module shows the existing concentration of strip to be measured in jump page, and reception is checked oneself report and asked
It asks, uploading detection result is asked to relatives' request and uploading detection result to doctor；
Step f, mobile phone application module receive the medical diagnosis result and medical treatment guidance opinion of physician feedback, notice detection knot
Fruit uploader downloads medical diagnosis result and medical treatment guidance opinion.
The beneficial effects of the invention are as follows：
(1) it can ensure the accuracy and validity of quantitative detection；
(2) patient from far-off regions can be facilitated to see a doctor, realize tele-medicine, optimize medical treatment configuration；
(3) mitigate working doctor amount, by the automatically derived concentration of software, without artificial chemical examination；
(4) medical treatment cost is reduced.
Invention is further described in detail with reference to the accompanying drawings and embodiments；But the present invention's is a kind of based on mobile phone
Immunity-chromatography test strip quantitative detection system and method are not limited to embodiment.
Description of the drawings
Fig. 1 is the structural schematic diagram of present system；
Fig. 2 is the flight data recorder internal structure schematic diagram of present system；
Fig. 3 is mobile phone application module page Fig. 1 of the present invention；
Fig. 4 is mobile phone application module page Fig. 2 of the present invention；
Fig. 5 is the work flow diagram of the present invention.
Specific implementation mode
A kind of immunity-chromatography test strip quantitative detection system based on mobile phone of the present invention, including image acquisition hardware device and hand
Machine application module, the mobile phone are smart mobile phone, include camera and convex mirror, and can realize mobile phone by software programming
The function of application module.
Shown in referring to Fig. 1 and Fig. 2, described image acquisition hardware device includes mobile phone 101, elastic fixing clip 102, slides and lead
Rail 103 and flight data recorder 104.The rail plate 103 is fixedly connected with elastic fixing clip 102；The elastic fixing clip 102 can root
It is moved according to the width of mobile phone 101 and the position of camera；The flight data recorder 104 can slidably reciprocate directly in rail plate 103
The camera position of mobile phone 101 is directed to the aperture 205 of flight data recorder.Be close to 101 camera of mobile phone is a piece of convex mirror
206, it is focused for mobile phone 101.
Described image acquisition hardware device further includes magnetic strip fixed block 201, conventional strip 202, scattering sheet 203 and green
Light LED light 204.The flight data recorder 104 is ferrous material, and inner top is equipped with the slot of one side opening for the strip
202 are inserted into, and the slot bottom is equipped with its detection window when opening makes the strip 202 be inserted into and is not blocked.The magnetic examination
Fixed block 201 is placed on the bottom of the strip 202, since flight data recorder 104 is ferrous material, the magnetism strip fixed block
Strip 202 can be fixed on flight data recorder inner top by 201, avoid its shaking.The green light LED lamp 204 includes two, is placed
It, can to adjust lamplight brightness, (LED light be by being attached to button below according to external environment in the flight data recorder 104 above bottom
Battery powered).The scattering sheet 203 includes two, is individually positioned in two green light LED lamps, 204 top, two symmetrical scattering sheets
203 enable light to be evenly distributed in strip 202.Because of the position difference of different mobile phone cameras, it is adjusted
It is inserted into the depth of strip 202, the mobile flight data recorder 104 on guide rail 103 so that camera, aperture and strip detection window are in
Same straight line, the image acquisition hardware device can obtain the image of clear high resolution.
The mobile phone application module includes image capturing unit, image processing unit, concentration value computing unit, testing result
Uploading unit and medical treatment guidance opinion download unit.
Described image shooting unit can analyze current image quality quality, when imaging meets accessible regulation, carry
Show that user can shoot；If being unsatisfactory for processing requirement, image can not be shot.
Described image processing unit includes image gray processing, noise reduction deblurring, extraction detection window and extraction detection line
And nature controlling line.
Described image gray processing includes that the strip image that will have been shot carries out gray processing processing, makes the value of each pixel
In 0 to 255 section.
The noise reduction deblurring includes removing image using weighted mean filter and medium filtering for strip feature of image
In Gaussian noise and salt-pepper noise.
The extraction detection window includes：First rough detection window edge is determined using edge detection operator；It is right again
The pixel grey scale of specific region carries out accumulation calculating, obtains the boundary coordinate of detection window, is carried from image according to boundary coordinate
Take out detection window.
The extraction detection line and nature controlling line include being directed to detection window and strip feature of image, use adaptive threshold
Fuzzy saltus step cell neural network algorithm, extracts the detection line and nature controlling line of strip.
Detection line and nature controlling line information are quantified, are passed through according to langbobier law by the concentration value computing unit
The working curve of characteristic value and concentration after quantization obtains the concentration levels of strip to be measured.
The testing result uploading unit and medical treatment guidance opinion download unit can realize the message interconnection sum number between doctors and patients
It is shared according to base resource.Its detection information can be uploaded to database by patient after login account, facilitate medical staff and relatives
Solve patient profiles.Medical staff for sufferer testing result after making medical diagnosis, by medical treatment guidance opinion and detailed inspection
It observes and predicts announcement and is uploaded to database.Sufferer and relatives will receive the information push for coming from application module later, notify it in time
Medical treatment guidance opinion and examining report are downloaded, to carry out the diagnosis and treatment of next step.
It is illustrated in figure 3 mobile phone application module page Fig. 1 of the present invention, when the prompt 303 for occurring to shoot now,
It clicks and determines 302, application module starts to identify that image carries out image procossing, calculates concentration value；It can be with to abandon this time shooting
Cancellation 301 is clicked to adjust again.
It is illustrated in figure 4 mobile phone application module page Fig. 2 of the present invention, existing concentration is shown in application module upper right side
401, patient, which can click, checks oneself report 402, this application module is automatically connected in hospital database, can be found according to concentration and oneself
Oneself related indication diagnosis report greatly facilitates patient to understand the state of an illness, without waiting in line, for suffering from same disease for a long time
Patient, such as diabetic learn whether the state of an illness improves without going to hospital to register, only need to be in using the present invention it can be learnt that
Concentration understands own situation by comparison；On the other hand, if itself can not accurate analysing patient's condition, or have Dyslexia
Personage can select to be sent to doctor 404 or be sent to relatives 403, and doctor is according to medical experience and hospital database information point
Conditions of patients is analysed, and related advisory can be provided, patient is allowed to take drug and the intake that is careful in one's diet.Greatly facilitate remote mountain
The resident in area sees a doctor, and optimizes medical treatment configuration.
As shown in figure 5, a kind of immunity-chromatography test strip quantitative detecting method based on mobile phone, includes the following steps：
Mobile phone camera is opened, elastic force geometrical clamp is fixed on phone housing, elastic force geometrical clamp is moved left and right and to image
The aperture of head and flight data recorder is located at same plummet face；Allow flight data recorder along rail plate sliding until the aperture alignment of flight data recorder is taken the photograph
As head, it is fixed on rail plate with screw and avoids shaking；It is then inserted into detection strip, sliding strip makes detection window just
To camera, chromatography strip is fixed in flight data recorder using magnetic strip fixed block (such as magnet) and avoids shaking, is finally imaged
Head, aperture and detection window should be in line；Two LED light are opened, adjust brightness until light is soft to be uniformly distributed in detection
Window；
Opening mobile phone application module occurs " focusing and failing, please again if focusing failure in the mobile phone screen upper right corner
Focusing " printed words；There is " can be shot now " word to defocused, detection window imaging clearly, the mobile phone screen upper right corner in success
Sample presses shooting, you can obtains clearly pending image；
It clicks and determines, application module brings into operation image processing function, including：Using the pending image of shooting as input
Carry out gray processing；The noise reduction of image is carried out using weighted mean filter and medium filtering；Use edge detection operator and given zone
The strategy extraction detection window of domain pixel grey scale accumulation calculating；Use the fuzzy saltus step cell neural network algorithm of adaptive threshold
Extract detection line and nature controlling line；Detection line and nature controlling line information are quantified, characteristic value after quantization and concentration are passed through
Working curve obtains the concentration levels of strip to be measured；
The upper right side of mobile phone jump page, screen shows existing concentration, and to check oneself report button, (patient can basis for centre
The information that various concentration represents in the database of hospital is compared with oneself existing concentration), it can also select to be sent to relatives,
The two buttons of doctor, their possible possibly can not accurately understand some professional terms for the elderly, can select
Relatives are sent to, doctor is sent to by it.
Doctor receive information push after, according to medical experience and concentration data library obtain examining report feed back to patient and
In relatives' hand, if there is something special, patient can also be contacted according to the patient's number provided in database.
A kind of immunity-chromatography test strip based on mobile phone that above-described embodiment only is used for further illustrating the present invention quantitatively detects
System and method, but the invention is not limited in embodiments, it is every that above example is made according to the technical essence of the invention
Any simple modification, equivalent change and modification, each fall in the protection domain of technical solution of the present invention.
Claims (6)
1. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone, which is characterized in that including：Mobile phone (101), elasticity are solid
Clamp (102), rail plate (103), flight data recorder (104) and strip (202)；The elastic fixing clip (102) and the mobile phone
(101) it is connected for being moved according to the width of mobile phone (101) and the position of camera；The rail plate (103) and institute
Elastic fixing clip (102) is stated to be fixedly connected；The flight data recorder (104) is slidably disposed on the rail plate (103)；Institute
It includes camera and application module to state mobile phone (101), and the application module includes carrying out image taking to the strip (202)
Image capturing unit, the image processing unit handled to the image of shooting carry out concentration value calculating to processing result image
Concentration value computing unit, testing result uploading unit that concentration value is sent and medical treatment guidance opinion download unit；Institute
It states strip (202) and is fixed adjustably to the flight data recorder (104) inner top；Flight data recorder (104) bottom is equipped with an aperture
(205)；When the application module carries out image taking, the detection window of the camera, aperture (205) and strip is in same
Straight line；
The flight data recorder (104) is ferrous material；
Flight data recorder (104) inner top is equipped with the slot of one side opening for the strip (202) insertion, the slot bottom
Portion is equipped with opening；
The quantitative detection system further includes a magnetic strip fixed block (201)；The magnetism strip fixed block (201) is placed on
Strip (202) bottom is used to the strip (202) being fixed to the irony flight data recorder (104).
2. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 1, it is characterised in that：Institute
It is magnet to state magnetic strip fixed block (201).
3. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 1, it is characterised in that：Institute
It further includes a convex mirror (206) to state mobile phone；The convex mirror (206) is close to the camera and focuses for mobile phone.
4. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 1, it is characterised in that：Institute
It states and is placed with two green light LED lamps (204) above the interior bottom of flight data recorder (104).
5. a kind of immunity-chromatography test strip quantitative detection system based on mobile phone according to claim 4, it is characterised in that：Institute
It states and is placed with scattering sheet (203) respectively above two green light LED lamps (204).
6. a kind of immunity-chromatography test strip quantitative detecting method based on mobile phone is based on mobile phone based on one kind described in claim 4
Immunity-chromatography test strip quantitative detection system, which is characterized in that including：
Step a opens mobile phone camera, elastic fixing clip is fixed on phone housing, elastic fixing clip is moved left and right and to take the photograph
As the aperture of head and flight data recorder is located at same plummet face；By flight data recorder along rail plate sliding until the aperture of flight data recorder is aligned
Camera is fixed with screw；It is then inserted into detection strip, sliding strip makes its detection window face camera, utilizes magnetic
Strip is fixed in flight data recorder by property strip fixed block to avoid shaking；Two green light LED lamps are opened, adjust brightness until light is soft
Be evenly distributed on detection window；
Step b, mobile phone application module, which receives, starts request, if focusing failure, shows that " focusing is lost in the mobile phone screen upper right corner
It loses, please focuses again "；If focused successfully, detection window imaging clearly, then showing in the mobile phone screen upper right corner " now can be with
Shot ", after receiving shooting request, taken clearly pending image；
Step c is received after determining request, and mobile phone application module carries out image processing unit and concentration value calculates, including：It will clap
The pending image taken the photograph carries out gray processing as input；The noise reduction of image is carried out using weighted mean filter and medium filtering；Make
With the strategy extraction detection window of edge detection operator and specific region pixel grey scale accumulation calculating；Use the mould of adaptive threshold
Paste saltus step cell neural network algorithm extracts detection line and nature controlling line；Detection line and nature controlling line information are quantified, passed through
The working curve of characteristic value and concentration after quantization obtains the concentration levels of strip to be measured；
Step d, mobile phone application module show the existing concentration of strip to be measured in jump page, and receive check oneself report request, on
Testing result is passed to ask to doctor to relatives' request and uploading detection result；
Step f, mobile phone application module receive the medical diagnosis result and medical treatment guidance opinion of physician feedback, notify in testing result
Biography person downloads medical diagnosis result and medical treatment guidance opinion.
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| CN110220891A (en) * | 2019-05-24 | 2019-09-10 | 华南农业大学 | A kind of portable mycotoxin immunochromatography quantitative testing device and detection method |
| CN111402347A (en) * | 2020-03-20 | 2020-07-10 | 吴刚 | New crown pneumonia epidemic situation prevention and control system based on Internet of things |
| US10991190B1 (en) | 2020-07-20 | 2021-04-27 | Abbott Laboratories | Digital pass verification systems and methods |
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| CN105181959A (en) * | 2015-06-25 | 2015-12-23 | 中国科学院电子学研究所 | Immunochromatography test strip quantitative detection apparatus and immunochromatography test strip quantitative detection method |
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