CN106525826A - Molecule smart-phone rapid test method based on colorful dominant wavelength and complementary wavelength - Google Patents
Molecule smart-phone rapid test method based on colorful dominant wavelength and complementary wavelength Download PDFInfo
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- CN106525826A CN106525826A CN201610914030.2A CN201610914030A CN106525826A CN 106525826 A CN106525826 A CN 106525826A CN 201610914030 A CN201610914030 A CN 201610914030A CN 106525826 A CN106525826 A CN 106525826A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
The invention discloses a molecule smart-phone rapid test method based on the colorful dominant wavelength and the complementary wavelength. The rapid test method is used for preparing a sample capable of generating color substances, is used for a device for collecting developing product pictures and a smart-phone detection procedure of the colorful dominant wavelength and the complementary wavelength of a solution and displaying the colorful dominant wavelength or the complementary wavelength corresponding to different solutions. The molecule smart-phone rapid test method is used for detecting the colorful dominant wavelength and the complementary wavelength of a colorimetric-analysis smart phone, rapid quantitative detection of biological chemistry molecules is achieved, and the molecule smart-phone rapid test method has the advantages of being easy and convenient to operate, low in cost, wide in application range, suitable for majority users and the like.
Description
Technical field
The present invention relates to a kind of smart mobile phone fast determining method of colorimetric analysis, specifically, be it is a kind of by testing sample with
In the clean transparent reaction vessels of the mixed color product solution injection of developer, then it is placed under LED area light source and uses
Smart mobile phone is taken pictures, and detects that program calculates the dominant wavelength of solution colour to be measured or complementary wavelength by smart mobile phone, real
The existing quantitative detecting method to sample relevant parameter.
Background technology
With the raising of socio-economic development, scientific progress and living standards of the people, water quality of the people to Drinking Water
Require to improve constantly, it is therefore proposed that a kind of water quality method for quick is particularly important.The index of water quality detection is included very
Many, wherein, Drinking Water mainly considers the impact to health, and its water standard includes that some physical indexs, chemistry refer to
Mark and microbiological indicator.The concentration of pH and nitrite is the important index of two of which, and different indexs has different standards,
The pH scopes for such as requiring water sample are 6.5 ~ 8.5, and the toxicologic index of nitrite is 1mg/L etc..Detection water quality index has not
Same detection method.Micro constitutent in water mainly includes red, orange, green, blue, yellow (ROGBY) mainly with water quality detection instrument analyzing, wherein, has
Gas chromatography, liquid chromatography, high performance liquid chromatography, chromatography of ions etc..The method for also having some indexs conventional has point
Light photometry, analysis by titration, gravimetry, electrochemical methods etc..Though such detection method has more accurate inspection
Result is surveyed, but its testing cost is high, it is long to take, need professional to operate, it is also limited to some large-scale testing agencies, it is impossible to general
And in the middle of family of domestic consumer.
In recent years, colorimetric method is used widely in quick detection.Colorimetric method is a kind of quantitative analysis method, general to wrap
Include two steps：One is to select appropriate developer and component reaction to be measured, forms colored compound；Two is to compare or measure to have
The color depth of color compound, and then determine the content of component to be measured.It is in the research of quick detection and the handsome choosing of early stage, a kind of
New colorimetric analysis is that the application of digital picture colorimetric method is more and more extensive, and its principle includes IMAQ and picture processing two
Individual part.Conventional IMAQ instrument has scanner, CCD or cmos imaging instrument, digital camera, smart mobile phone etc.；And process
Picture then according to the characteristics of chromogenic reaction selecting using different color model, this process by professional photo handling software or
Write mobile phone A PP to complete.With optical colorimetry compared with photoelectric colorimetry, this colorimetric method is easy to operate, it is time-consuming less, cost
It is low, accuracy of detection between optical colorimetry and photoelectric colorimetry, therefore, its application receive significant attention.
For digital picture colorimetric method, in recent years, researcher develops some method for quick in succession.Such as Meng etc.
People（X. Meng, C. W. Schultz, C. Cui, X. Li, H. Yu, On-site chip-based
colorimetric quantitation of organophosphorus pesticides using an office
scanner,Sensors and Actuators B: Chemical, 2015, 215: 577-583）Swept using ordinary flat
The digital picture that instrument obtains the residual detection liquid of agriculture in microfluidic channel is retouched, the Y value of picture is then analyzed, completes residual to agriculture to determine
Amount detection.Li et al.（ Li X, Tian J F, Shen W. Anal Bioanal Chem, 2010, 396(1): 495-
501.）Detection water sample Nitrite ion concentration when, in Photoshop by picture from RGB patten transformations be grayscale mode,
And then the relation between analysis gray-scale intensity and nitrite ion concentration.Richard et al.（Murdock R C, Shen L,
Optimization of a paper-based ELISA for a human performance biomarker.
Analytical chemistry, 2013, 85(23): 11634-11642.）Obtained in filter paper microchannel using panel computer
The digital picture of thing to be checked, realizes IgG in human saliva by analyzing the change of rgb value（Immunoglobulin (Ig)）Detection.
However, above-mentioned detection method restricted application, for tone occurs the chromogenic reaction of significant change, analyzes RGB
Value, gray value or CMY values are just no longer suitable for.And Abe et al.（K. Abe , K. Suzuki, D.Citterio, Inkjet-
printed microfluidic multianalyte chemical sensing paper. Analytical
chemistry, 2008, 80(18): 6928-6934）Microfluidic channel is prepared on filter paper using inkjet technology,
Picture collection is carried out to the colour developing on filter paper by scanner, then by Lab colorimeter systems analyzing color, it is achieved thereby that
Detection to Multiple components in human urine.Shen et al.（Shen L, Hagen J A, Papautsky I, Point-of-
care colorimetric detection with a smartphone[J]. Lab on a Chip, 2012, 12
(21): 4240-4243.）The three-dimensional relationship between different pH value and the chromaticity coordinate x-y of its color product is established, and it is this
Three-dimensional relationship it is determined that sample pH value when can be relatively complicated.Yetisen et al.（Yetisen A K, Martinez-
Hurtado J L, Garcia-Melendrez A, A smartphone algorithm with inter-phone
repeatability for the analysis of colorimetric tests[J]. Sensors and
Actuators B: Chemical, 2014, 196: 156-160.）Each standard items is developed the color on 1931 chromaticity diagrams of CIE
The line of the chromaticity coordinate point of color is used as normal line afterwards, by related algorithm be calculated sample pH value and protein it is dense
Degree.Li Haiyan et al.（Li Haiyan, Zhang Jiaoliang, Li Xiaochun：Colorimetric determination based on dominant wavelength and complementary wavelength is detected；
Analysis test journal, 2016,11）MATLAB is combined to pH value of solution and NO using the dominant wavelength and complementary wavelength of color2 -1Concentration is entered
Row quantitative determination.
Above method of operation in operation step is more, time-consuming for detection, and IMAQ is separately carried out with image procossing, therefore uncomfortable
Close field quick detection, some remote districts or or the area that relatively falls behind launch water quality detection work it is also more difficult.
And it is widely available with smart mobile phone, smart mobile phone is applied to detection by increasing researcher, and by IMAQ
It is integrated in one with image procossing.Such as Lopez-Ruiz et al.（ N. Lopez-Ruiz, V. F. Curto ,M .M
Erenas, Smartphone-based simultaneous pH and nitrite colorimetric
determination for paper microfluidic devices. Analytical chemistry, 2014, 86
(19): 9554-9562）Picture collection is carried out to the colour developing in microchannel on filter paper with mobile phone, afterwards by cell phone software to figure
Piece analysis realizes the quantitative determination of pH value and nitrous acid.Shen et al.（L. Shen , J .A. Hagen , I.
Papautsky , Point-of-care colorimetric detection with a smartphone. Lab on a
Chip, 2012, 12(21): 4240-4243）Picture collection is carried out using smart mobile phone, different pH value is established and is developed the color with which
Three-dimensional relationship between the chromaticity coordinate x-y of product.St é fani et al.（Andrade S I E, Lima M B, Barreto
I S,A digital image-based flow-batch analyzer for determining Al (III) and Cr
(VI) in water.Microchemical Journal, 2013, 109:106-111.）It is being equipped with using IP Camera
Take pictures in the closed enclosure of fluorescent lamp, by analyzing RGB values, realize the detection of aluminium ion and chromium ion in running water.Which has
Inexpensive, relatively time-consuming short advantage, but it is relatively complicated when processing.Proposed based on this present invention a kind of based on smart mobile phone
Using the digital picture colorimetric method of dominant wavelength or complementary wavelength, using smart mobile phone and APP is write so that testing result
Directly it is presented on mobile phone interface, highly shortened detection time.
The content of the invention
It is an object of the invention to provide a kind of molecular intelligence mobile phone fast determining method based on dominant wavelength and complementary wavelength,
Realization fast and accurately detected to biochemical samples anywhere or anytime based on smart mobile phone, and with easy to operate, into
This is cheap, the characteristics of applied widely.
The detection reaction vessels mentioned in the present invention are glass cuvette, PDMS microchannels or other clean transparent devices
Ware.Realize that the technical scheme taken by above-mentioned purpose is as follows.
A kind of molecular intelligence mobile phone fast determining method of base based on dominant wavelength and complementary wavelength, the fast determining method be by
What the following steps were carried out：
For the sample preparation of color product can be generated；
For gathering the device of color product picture；
For the smart mobile phone detection program of solution colour dominant wavelength and complementary wavelength, the main ripple of the corresponding color of different solutions is shown
Long or complementary wavelength；
Further, supplementary technology scheme is as follows：
The preparation for generating the sample of color product is by can generate color product and product shade has bright
The sample of aobvious change is constituted.
The color product picture is, under LED area light source, to be taken pictures by the camera figure reaction vessels of smart mobile phone
Obtain.
The fast detection method is the condition for making sample that complete chromogenic reaction can occur in detection sample reaction vessels
Under, using smart mobile phone App to taking pictures to reaction vessels, mobile phone A pp intercepts after reaction to obtain picture, picture is carried out point
Analysis, realizes Quantitative detection.
Realize a kind of smart mobile phone dominant wavelength and complementary wavelength based on colorimetric analysis that the invention described above is provided
Fast determining method, testing sample to be measured and corresponding developer are mixed in reaction vessels ware, LED area light source is then placed on
Under, which is taken pictures using smart mobile phone camera, and be analyzed by the APP for having write and fit Calibration equation and obtain
One testing result of testing sample solution, is shown in result interface.Dominant wavelength and complementary wavelength of the present invention using color,
And program is detected using smart mobile phone, realize the detection to solution relevant parameter.This fast determining method has low cost, operability
By force, the features such as detection time is short.Therefore there is good application prospect in fields such as environment measuring, water quality detections, it is particularly possible to should
Use the detection such as ambient water quality of remote districts.
Description of the drawings
Fig. 1 is present invention detection microchannel transparent base layer.
Fig. 2 is present invention detection microchannel transparent channel layer.
Fig. 3 is present invention detection microchannel transparent covering layer.
Fig. 4 is that the present invention adds color product.
Fig. 5 is smart mobile phone detection program runnable interface of the present invention.
In figure：1：Transparent base layer；2：Transparent channel layer；3：Transparent covering layer；3-1：Color product well；3-2：It is aobvious
Color product passage；4：Liquid-transfering gun；5：Smart mobile phone detects program runnable interface；5-1：Main interface；5-2：Preview interface；5-3：
Result interface.
Specific embodiment
The specific embodiment that exemplary embodiment further illustrates the present invention is made below.
Embodiment 1
Implement a kind of molecular intelligence mobile phone fast determining method based on dominant wavelength and complementary wavelength, comprise the following steps：
（1）The preparation of different pH value：0.2mol/L sodium dihydrogen phosphates are prepared with AMSP, citric acid is matched somebody with somebody
Then the two mixed and pH value be obtained from 4.0 to 8.5 by 0.1mol/L solution processed in varing proportions（PH intervals 0.5）Solution.
0.1mol/L sodium bicarbonate solutions and 0.1mol/L sodium carbonate liquors is prepared respectively with sodium acid carbonate and sodium carbonate, and the two is with difference
Ratio mixing is obtained the solution that pH value is 9.0,9.5,10.0.The pH value of all solution is surveyed by 3100 pH meters of STARTER
It is fixed.
（2）The preparation of pH developers：Thymol blue 0.01g, methyl red 0.32g, Bromothymol blue 1.20g is taken respectively,
The two is mixed and is ground well by phenolphthalein 1.20g, is taken anhydrous alcohol solution that 200mL concentration is 95% fully, and is added 150mL ultrapure
Water dilutes.Finally with NaOH prepare 0.1mol/L sodium hydroxide solution come neutralize previous solu to just show green,
And add ultra-pure water constant volume to 400mL.
（3）The solution of different pH value and the mixing of developer：The cushioning liquid of 3mL difference pH value is respectively placed in into colorimetric cylinder
Interior, the nitrite ion for taking 0.1mL is added thereto, and shakes up so as to which chromogenic reaction fully occurs.Subsequently, take the injection of 1mL color products
In the microchannel for designing.
（4）To sample Uniform Illumination：Above-mentioned PDMS microchannels are placed on into LED area light source（18*18cm, 20W）Under.
（5）Smart mobile phone detects program reading process：The cuvette first smart mobile phone being fixed under LED area light source
Front.Click on icon on mobile phone screen and enter main interface, main interface includes four buttons, respectively Start, History,
Instructions、Quit.Click on Start buttons and enter mobile phone camera preview interface, one now in preview interface, can be shown
Individual big red rectangle frame, is dispersed with the narrow rectangle frame of six redness again in this rectangle inframe, each rectangle frame is aligned preview circle
After bar-shaped frame on face, Capture buttons are clicked on, program can intercept automatically the picture of red rectangle inframe and be saved in mobile phone
In, Analyse buttons are then clicked on, program can calculate the dominant wavelength of each bar shaped inframe color automatically, and according to titer
PH value and corresponding dominant wavelength fit Calibration equation, with reference to this Calibration equation dominant wavelength corresponding with liquid to be checked
The pH value of testing sample solution is calculated, is finally directly displayed testing result on interface.
Embodiment 2
Method in embodiment 1 is used for detect the concentration of water Nitrite ion, is comprised the following steps：
（1）The preparation of the sodium nitrite solution of variable concentrations：The natrium nitrosum powder of 0.025 g is taken, the ultrapure of 25 mL is dissolved into
In water, the sodium nitrite solution of 1000 mg/L is obtained.As mother liquor, respectively compound concentration be 0,1,2,5,10,12,15,
20th, the sodium nitrite solution of 25,30,40 mg/L.
（2）The preparation of nitrite developer：Take 0.1722 g sulfanilamide (SN), 0.0518 g hydrochloride naphthodiamides and 0.317g lemons
Lemon acid, is dissolved in the methyl alcohol of 20 mL, keeps in dark place at low temperature before use.
（3）The natrium nitrosum of variable concentrations and the mixing of developer：The sodium nitrite solution of 3mL variable concentrations is put respectively
In colorimetric cylinder, the nitrite ion for taking 0.7mL is added thereto, and shakes up so as to which chromogenic reaction fully occurs.Subsequently, 1mL colour developings are taken
In the microchannel that product injection is designed.
（4）To sample Uniform Illumination：Above-mentioned PDMS microchannels are placed on into LED area light source（18*18cm, 20W）Under.
（5）Smart mobile phone detects program reading process：The cuvette first smart mobile phone being fixed under LED area light source
Front.Click on icon on mobile phone screen and enter main interface, main interface includes four buttons, respectively Start, History,
Instructions、Quit.Click on Start buttons and enter mobile phone camera preview interface, one now in preview interface, can be shown
Individual big red rectangle frame, is dispersed with six red narrow bar frames again in this rectangle inframe, each rectangle frame is aligned preview circle
After bar-shaped frame on face, Capture buttons are clicked on, program can intercept automatically the picture of red rectangle inframe and be saved in mobile phone
In, Analyse buttons are then clicked on, program can calculate the complementary wavelength of each bar shaped inframe color automatically, and according to standard
The NO of liquid2 -1Concentration and corresponding color complementary wavelength fit Calibration equation, with reference to this Calibration equation face corresponding with liquid to be checked
Color complementary wavelength calculates the NO of testing sample solution2 -1Concentration, finally directly displays testing result on interface.
Claims (5)
1. a kind of molecular intelligence mobile phone fast determining method based on dominant wavelength and complementary wavelength, the fast determining method is by following
What step was carried out：
For the sample preparation of color product can be generated；
For gathering the device of color product picture, picture is gathered for taking photograph of intelligent mobile phone；
For the smart mobile phone detection program of solution colour dominant wavelength and complementary wavelength, the main ripple of the corresponding color of different solutions is shown
Long or complementary wavelength.
2. fast determining method as claimed in claim 1, the fast determining method follow these steps to carry out：
For the sample preparation of color product can be generated, it is with the change tone of the relevant parameter of sample by its color product
The sample that significant change occurs is constituted；
For being put into equipped with sample to be detected under the device of the collection color product, the aobvious of sample amounts detection is realized
Colour response, the color of example reaction vessel are able to be read corresponding data result by mobile phone reading device, after intercepting reaction
Pictorial information, the picture to obtaining is analyzed, and by compared with results of comparison, shows testing result；
The smart mobile phone detection program for solution colour dominant wavelength and complementary wavelength, shows testing result, is by Java
Language is write and is operated on Android phone platform.
3. fast determining method as claimed in claim 1, the reaction vessels are cuvette, PDMS microchannels or other see-through looks
Put.
4. fast determining method as claimed in claim 1, the device of the collection color product picture, adopt for taking photograph of intelligent mobile phone
Collection picture, is that the reaction vessels that will be equipped with color product are placed under LED area light source and complete.
5. fast determining method as claimed in claim 1 or 2, the fast detection method is sample is occurred in example reaction vessel
Completely under conditions of chromogenic reaction, the reaction vessels after chromogenic reaction are taken pictures using mobile phone A pp, cut according to mobile phone A pp
Negate should after picture, picture is analyzed, Quantitative detection is realized.
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Cited By (5)
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| CN107727651A (en) * | 2017-08-29 | 2018-02-23 | 太原理工大学 | The quick high-precision fixed amount detecting devices of pH in scene and method based on smart machine |
| CN107917876A (en) * | 2017-10-16 | 2018-04-17 | 太原理工大学 | Antibiotic detection device and method based on nanogold aptamers structure |
| CN109254000A (en) * | 2018-10-25 | 2019-01-22 | 太原理工大学 | Array urine multiple determination apparatus and method based on smart machine colorimetric analysis |
| JP2020525793A (en) * | 2017-06-27 | 2020-08-27 | ライフ テクノロジーズ ホールディング プライベート リミテッド | Method for analyzing a liquid sample, microplate reader, and computer program |
| CN114264604A (en) * | 2021-12-23 | 2022-04-01 | 国家体育总局体育科学研究所 | Athlete urine color analysis system |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2020525793A (en) * | 2017-06-27 | 2020-08-27 | ライフ テクノロジーズ ホールディング プライベート リミテッド | Method for analyzing a liquid sample, microplate reader, and computer program |
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| CN107727651A (en) * | 2017-08-29 | 2018-02-23 | 太原理工大学 | The quick high-precision fixed amount detecting devices of pH in scene and method based on smart machine |
| CN107917876A (en) * | 2017-10-16 | 2018-04-17 | 太原理工大学 | Antibiotic detection device and method based on nanogold aptamers structure |
| CN107917876B (en) * | 2017-10-16 | 2020-12-22 | 太原理工大学 | Antibiotic detection device and method based on nanogold-aptamer structure |
| CN109254000A (en) * | 2018-10-25 | 2019-01-22 | 太原理工大学 | Array urine multiple determination apparatus and method based on smart machine colorimetric analysis |
| CN114264604A (en) * | 2021-12-23 | 2022-04-01 | 国家体育总局体育科学研究所 | Athlete urine color analysis system |
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