CN101726587A - Instrument for indentifying and quantitatively analyzing image of immune test paper in moving process - Google Patents
Instrument for indentifying and quantitatively analyzing image of immune test paper in moving process Download PDFInfo
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- CN101726587A CN101726587A CN200810201133A CN200810201133A CN101726587A CN 101726587 A CN101726587 A CN 101726587A CN 200810201133 A CN200810201133 A CN 200810201133A CN 200810201133 A CN200810201133 A CN 200810201133A CN 101726587 A CN101726587 A CN 101726587A
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Abstract
The invention relates to an instrument for indentifying and quantitatively analyzing the image of a piece of immune test paper in the moving process, comprising a detected sample delivering and positioning device, an area array solid image sensor and an image identifying system. Compared with other static systems for quantitatively analyzing the image of a piece of immune test paper, the instrument can be used for consecutively and quantitatively analyzing the image of the immune test paper and is suitable for analyzing the bulk samples.
Description
The immune test paper conduct is the medical science detection means quickly and easily, and in vast family and hospital, places such as blood station are able to popularization and application, and constantly have more kind to be developed.But present immune test paper almost all is to qualitatively judge with naked eyes, and quantitative information can not be provided.And in medical science detected, a lot of projects had both needed in time to detect quickly and easily, also needed quantitative detection information.If can solve the detection by quantitative problem of immune test paper, will being accepted by vast clinical detection department and family more widely of immune test paper will detect a developing direction that gets a good chance of of industry for medical science.
The immune test paper detection technique is the same with enzyme linked immunological absorption (ELISA), has antibody antigen and reacts peculiar high specific and high sensitivity.Both reaction methods are identical, as sandwich method, and indirect method, competition law etc.Different is, ELISA is a liquid-phase reaction system, and the signal power represents that by the depth of solution colour in the hole running time is long, is fit to batch processing.And immune test paper is the immobilon-p reaction, and the signal power is represented that by the shade of lines on the film or some reaction is fit to on-the-spot instant the detection fast.If can carry out quantitative test to the shade of lines or point, then just can just carry out discriminatory analysis to concentration of reactants.
To main three kinds of the method that immune test paper carries out quantitative test, a kind of is to add magnetic bead in label, utilizes magnetic field detector that reaction zone is carried out signal processing and analysis.The optical signalling that produces during a kind of irradiation that detects the specific wavelength light beam obtains the strong and weak signals of line color on the film.Another kind method is exactly to utilize image-forming component to obtain the associated picture signal of immune test paper, through relevant data analysis, draws the feature quantitative information of reaction zone, and the data computation mode by specific draws quantitative analysis results.On the imaging quantitative test direction of test paper, there are many technical schemes at present.
Patent 200410034105.5,200420049579.2nd adopts the infrared excitation light source, and through excitation light path, phosphor pattern receiving light path and image processing system are formed.Its exciting light line focus becomes line, is radiated on the up-converting phosphor immune chromatography test paper, after particular optical is handled, carries out coherent signal by the image-forming block collection then, and the transmitted image disposal system is analyzed.
Patent 200510030549.6 is by associated optical system laser to be divided into two two-beams that certain intervals arranged, be radiated at respectively on the detection band and quality control band of test paper, after exciting, by related optical component optical signalling is sent into one dimension CCD element, the signal with one dimension CCD carries out data analysis by relevant single-chip microcomputer then.
Patent 03137101.9 is to adopt the contact type image scanner to obtain the colloid gold chromatographic test paper image, by mould/number conversion, the signal digital of the optical density that obtains is used for the correlated digital disposal system analyzes.
Patent 03154445.2 also adopts the image scan element to obtain the digitized video of test paper lines, carries out the correlated characteristic analysis by comprising digital signal processor at interior microprocessor, and the neural dosing unit of utilization network carries out discriminatory analysis.
People such as the Master degree candidate Zhang Feng of Jilin University have also announced their development in the relevant thesis of delivering in 2005.Promptly adopt video CCD as image acquisition component, carry out mould/number conversion by the video image processing module after, be that single-chip microcomputer W77E58 carries out analysis of image data and handles by 51 of enhancement mode, and then carry out the result on the display module and show.This instrument also can pass through mode switch, as the effect of image pick-up card, by the USB mouth with image data transmission to PC, carry out the related data analyzing and processing.
In the multi-joint test paper analyser of urine, analytic target is the square thieving paper that is attached on the plastic strip, and this test paper is to be purpose with chemical color reaction.The urine test paper analyser is mainly analyzed the test paper color with light beam diffuse reflection analytical technology at present.Also have part producer to begin to adopt the digital image analysis technology, the image to the picked-up of the urine test paper in static state or moving process particular area carries out batch quantity analysis to this regional chemical reaction color.
The present invention is the image recognition and the quantitative analysis instrument of immune test paper in the moving process.Relative other static immune test paper quantified system analysis, the present invention can carry out the quantitative test of immune test paper continuously, is fit to the batch sample is analyzed.Characteristics of the present invention are:
1. analytic target of the present invention carries out the dynamic image picked-up in moving process.
2. analytic target of the present invention is the immune response signal that is in the immune test paper reaction zone, and both lines on the immune test paper also can be the points on the immune test paper.
3. image component of the present invention is a face battle array solid state image sensor (as CCD/CMOS), can obtain high-resolution digital picture apace.
4. among the present invention, the picture signal of solid state image sensor (as CCD/CMOS) picked-up is by usb protocol or directly be transferred to microprocessor, has omitted the mould/number conversion step of the video data between sensor and microprocessor.
5. the digital picture among the present invention is carried out image recognition and related data analysis by high speed microprocessor, draws concrete signal characteristic value.By the eigenwert of ad hoc approach reference standard sample, can analyze the immune substance concentration of concrete sample.
The specific embodiment explanation:
Affirm that with Taiwan the U30C type industry camera of Information Science Co., Ltd is as image acquisition element, 6 LED lamps are arranged as radiation source in its edge, camera and lamp source are placed in the box body of one five face closure, and its bottom is the travelling belt that has the immune test paper locating device.When immune test paper passed through the bottom of industrial camera with travelling belt, U30C carried out image acquisition to the test paper that is in the box body base plane.This test paper is to carry out the colloidal gold immune chromatography test that early pregnancy detects, and analytic target is the red lines of the C/T of test paper reaction zone.
The image recognition analytic system of this instrument be with the ARM9 of Korea S Samsung type S3C2410 processor as core, integrated 64M internal memory, the NORFlash of 2M, the NAND Flash of 64M, and comprise USB, LCD, the embedded IC plate of relevant external tapping such as serial ports.The digital rgb image that U30C gathers is transferred to S3C2410 by usb protocol.By being the analysis operation software of kernel with Linux, this digital picture is carried out specific discriminance analysis then, extract the eigenwert of the reaction C/T line color depth.
Arrange instrument that the HCG sample of serial concentration known is carried out image taking, draw the eigenwert of each test paper respectively, carry out curve fitting with concentration and its eigenwert of program specific in the instrument then, set up typical curve, be stored in the instrument series of samples.
The HCG test paper of getting unknown concentration then carries out image taking, draws its eigenwert by instrumental analysis, then with reference to the HCG typical curve of storage, draws the HCG concentration of this test paper.This result can show by the display screen on the instrument, also can carry out data by the printer that is connected with instrument and print preservation.
Claims (5)
1. this instrument detecting sample is the immune test paper of band special spectrum label, in the immune response district of test strips, can be by the main signal of line or some anabolic reaction, and the reaction main signal of being made up of line or point can be one, also can be a plurality of.
2. the special spectrum signal thing of this instrument detecting both can be a collaurum, also can be the label with other color, or other fluorescent/phosphorescent label.
3. the face battle array solid state image sensor of this instrument can be based on the CCD or the CMOS of face battle array.
4. the image identification system of this instrument is based on band high-speed computation function, internal memory and related management function microprocessor and the image recognition routine analyzer developed, this routine analyzer draws the eigenwert of immune test paper reaction signal with the correlated digital image analysis method, is purpose to analyze immunoreactive sample material concentration.
5. the microprocessor in this instrument had both comprised 16,32 all kinds of single-chip microcomputers and ARM, and microprocessors such as MIPS also comprise 64 and above computer computing machine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN200810201133A CN101726587A (en) | 2008-10-13 | 2008-10-13 | Instrument for indentifying and quantitatively analyzing image of immune test paper in moving process |
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| CN200810201133A CN101726587A (en) | 2008-10-13 | 2008-10-13 | Instrument for indentifying and quantitatively analyzing image of immune test paper in moving process |
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| CN101726587A true CN101726587A (en) | 2010-06-09 |
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| CN200810201133A Pending CN101726587A (en) | 2008-10-13 | 2008-10-13 | Instrument for indentifying and quantitatively analyzing image of immune test paper in moving process |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102279263A (en) * | 2011-05-06 | 2011-12-14 | 杭州顿力医疗器械股份有限公司 | CCD-type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers |
| CN103076449A (en) * | 2013-01-21 | 2013-05-01 | 中国检验检疫科学研究院 | Colloidal gold label detector |
| CN103197060A (en) * | 2013-03-24 | 2013-07-10 | 湖南远泰生物技术有限公司 | Autonomous recognition, positioning and quantitative detecting system and detecting method for colloidal gold immunochromatographic boxes |
| CN104954639A (en) * | 2014-09-23 | 2015-09-30 | 天津起跑线生物信息技术有限公司 | Digital-imaging-technology-based distributed medical detection system |
| CN105628912A (en) * | 2014-11-03 | 2016-06-01 | 天津市普瑞仪器有限公司 | Immune chromatographic analyzer |
-
2008
- 2008-10-13 CN CN200810201133A patent/CN101726587A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102279263A (en) * | 2011-05-06 | 2011-12-14 | 杭州顿力医疗器械股份有限公司 | CCD-type quantitative analysis system of colloidal gold immunity chromatography diagnosis test papers |
| CN103076449A (en) * | 2013-01-21 | 2013-05-01 | 中国检验检疫科学研究院 | Colloidal gold label detector |
| CN103197060A (en) * | 2013-03-24 | 2013-07-10 | 湖南远泰生物技术有限公司 | Autonomous recognition, positioning and quantitative detecting system and detecting method for colloidal gold immunochromatographic boxes |
| CN104954639A (en) * | 2014-09-23 | 2015-09-30 | 天津起跑线生物信息技术有限公司 | Digital-imaging-technology-based distributed medical detection system |
| CN105628912A (en) * | 2014-11-03 | 2016-06-01 | 天津市普瑞仪器有限公司 | Immune chromatographic analyzer |
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Application publication date: 20100609 |