CN106198406A - A kind of method of quick detection high whiteness flour wheat seed - Google Patents
A kind of method of quick detection high whiteness flour wheat seed Download PDFInfo
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- CN106198406A CN106198406A CN201610526685.2A CN201610526685A CN106198406A CN 106198406 A CN106198406 A CN 106198406A CN 201610526685 A CN201610526685 A CN 201610526685A CN 106198406 A CN106198406 A CN 106198406A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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Abstract
The method that the invention discloses a kind of quick detection high whiteness flour wheat seed, belongs to Semen Tritici aestivi flour whiteness detection technique field.Carry out as steps described below: every part of germ plasm resource takes wheat seed 100,15min is soaked with the phenol that mass concentration is 1.0%, taking out to be placed on is covered with in the culture dish that mass concentration is the filter paper that 1% phenol wet is crossed (ventral groove is downward), the filter paper that same phenol wet is crossed is covered on culture dish lid, building culture dish, put in the incubator of 37 DEG C, dye 25min, observing coloration result, said method is repeated 3 times；Standard of perfection: (1) achromophil seed is the wheat breed seed of high whiteness, the i.e. whiteness value wheat breed seed more than 80；(2) dye light brown or brown for low whiteness wheat breed seed, i.e. whiteness value wheat breed seed below 79.The present invention is convenient and swift, saves time quickly.Required instrument and equipment and the less minute of article only need 25min.
Description
Technical field
The invention belongs to Semen Tritici aestivi flour whiteness detection technique field, refer in particular to a kind of quickly high whiteness flour wheat seed of detection
Method.
Background technology
The method measuring Semen Tritici aestivi flour whiteness conventional at present has 2 kinds, and respectively whiteness instrument measures Flour whiteness with near
Infrared spectrum analyser measures Flour whiteness.Its operation principle and method are as follows:
(1) whiteness instrument measures Flour whiteness operation principle
Instrument utilizes integrating sphere to realize the measurement of absolute spectrum diffuse-reflectance rate, halogen tungsten lamp the light sent, through condenser lens
Becoming blue violet ray with light filter, enter integrating sphere, light, after integrating sphere inwall diffuse-reflectance, is radiated at the sample of test port
On, sample the light reflected is received by silicon cell after condenser lens, light hurdle color filter group, is converted into the signal of telecommunication.Separately have one
Road silicon cell receives the basal signal in spheroid.Two path signal is amplified respectively, mixed processing, measurement result digital-scroll technique.
Whiteness instrument Semen Tritici aestivi flour assay method step:
(1) weigh wheat seed 100g, use 130 DEG C to dry 40min after removing impurity and measure moisture.
(2) wheat wetting: after selected wheat cleaning, wheat wetting to Target moisture 15.0%, wheat wetting amount of water be=[(enters
Mill moisture-actual moisture)/(1-water into the mill)] × seed gross weight.Place pulverizing after 24h.
(3) pulverizing: shelling one time, reduction one time, calculate flour extraction.Laboratory is placed 1 week.
(4) measuring brightness: weigh 7.00g flour.Then Flour whiteness is measured with intelligence whiteness instrument.Measure its whiteness respectively
And record.
The shortcoming of the method is: sample is prepared loaded down with trivial details, and testing expenses are high, and technical difficulty is big, and the time is longer, and required instrument is relatively
Many and the most expensive, detection simultaneously needs grain weight big, limits the extensive application in basic unit's seed production, operation.
(2) near infrared ray reflective instrument measures the principle of Flour whiteness
It is based on reflectance spectrum technology that near-infrared measures the principle of Flour whiteness.During diffuse-reflectance, light and sample table
Face and interior molecules thereof interact.Sample surfaces is reflected again after finally carrying sample message, then anti-by detector detection
The optical signal penetrated, and then obtain unrestrained emission spectrum.Then the chemical score formed by test substance by traditional analysis method measures
Out, then by chemo metric software, the effective information of its spectrum is fully extracted, so by near infrared spectrum with
The composition information association of material is got up, and namely sets up a kind of qualitative or quantitative calibration model, finally near by unknown sample
The model that infrared spectrum input is built is predicted.
The method step of near-infrared analyzer mensuration Flour whiteness:
(1) sample is collected.Before setting up calibration model, first have to collect representative sample.Sample should be able to cover not
Know the content range that sample component is likely to occur, and the sample of each scope is preferably uniformly distributed, collect the content distribution of sample
The most then the suitability of institute's established model is the strongest.
(2) chemical analysis of sample standard value.Test material carries out chemical analysis by traditional method to sample component.
(3) collection of near infrared spectrum and pretreatment.The material of chemical score is had to carry out spectrum each setting up model
Gather.
(4) foundation of calibration model and checking.By chemometrics method set up test substance constituent content with
Quantitative calibration models between its spectrum or qualitative model, after calibration model is set up, it is necessary to verify it, to guarantee model
Accuracy and reliability.
(5) prediction unknown sample
After calibration model is set up, can be used for the analysis of unknown sample.By testing sample pulverizing, Flour milling method is surveyed with whiteness instrument
Determine Flour milling method.Then sample detection.
The shortcoming of the method: near-infrared spectrum technique is one indirectly analytical technology, and near infrared spectrum itself can not be right
The information such as the composition of sample, content are predicted, it is necessary to the chemistry formed by test substance by other traditional analysis methods
PH-value determination pH out, then sets up model by chemo metric software, and the near infrared spectrum of unknown sample inputs the mould built
The character of sample could be predicted by type.Need to consume substantial amounts of human and material resources and financial resources before setting up calibration model, in order to carry
The robustness of high model and the suitability, constantly should safeguard model and update.Instrument price is expensive simultaneously, it is difficult to general
And.
Summary of the invention
The present invention for overcoming above-mentioned the deficiencies in the prior art,
The method developing a kind of quick detection high whiteness flour wheat seed, is carried out as steps described below:
(1) every part of germ plasm resource takes wheat seed 100, soaks 15min with the phenol that mass concentration is 1.0%, takes out
It is placed on and is covered with in the culture dish that mass concentration is the filter paper that 1% phenol wet is crossed (ventral groove is downward), culture dish lid covers same
The filter paper that sample phenol wet is crossed, builds culture dish, puts in the incubator of 37 DEG C, and dye 25min, observes coloration result, above-mentioned side
Method is repeated 3 times.
Standard of perfection: (1) achromophil seed is high whiteness (whiteness value is more than 80) wheat breed seed.
(2) dye light brown or brown for (whiteness value is below 79) low whiteness wheat breed seed.
The accuracy rate of said method is 99%.
Advantages of the present invention
1. principle is reliable, and result is accurate.Phenol staining is the reaction of a kind of pure chemistry, it utilize the steam of phenol solution with
There is chemical reaction in the phenol oxidase in seed coat.The oxidase content of different kind kind Intradermal is different, Flour whiteness
High content is less, and the content that Flour whiteness is low is more.
The most convenient and swift, save time quickly.Required instrument and equipment and article less (incubator, culture dish, filter paper, tweezers) measure
Time only needs 25min.
3. convenient simplicity is measured, it is simple to penetration and promotion.The method is easy to for basic unit's scientific research breeding and little company breeder
Grasping, expense is relatively low simultaneously.It is easy to promote the use of in whole nation scientific research breeding unit.
The most with low cost.Phenol price is 20 yuan of 1 bottle of 500g, at least can use 500 times.Cost is 0.04 yuan every time.
Needed for test determination, instrument article are few simultaneously so that measure whiteness cost of consumption lower.
5. a reasonable Research approaches is provided for the detection of high whiteness flour purity.
6. the advantage in terms of scientific research breeding, the germ plasm resource with whiteness as criteria for classification is poor, is that whiteness genetic improvement exists
Difficulty in practice；The Wheat Germplasm Resources screening high whiteness be can quickly detect in this approach, Flour whiteness height, quality excavated
Excellent wheat germplasm, provides technical support for cultivating High quality glutenous wheat new varieties in high whiteness.And can be few at raw material, the used time
In the case of short, quickly recording the whiteness of all kind wheat flours, the detection for this index of whiteness provides the most convenient.
Accompanying drawing explanation
Fig. 1 utilizes this inventive method to detect 1-12 germ plasm resource coloration result；
Fig. 2 utilizes this inventive method to detect 12-24 germ plasm resource coloration result；
Fig. 3 utilizes this inventive method to detect 25--36 germ plasm resource coloration result；
Fig. 4 utilizes this inventive method to detect 37--48 germ plasm resource coloration result；
Fig. 5 utilizes this inventive method to detect 49-60 germ plasm resource coloration result；
Fig. 6 utilizes this inventive method to detect 61-72 germ plasm resource coloration result；
Fig. 7 utilizes this inventive method to detect 73-84 germ plasm resource coloration result；
Fig. 8 utilizes this inventive method to detect 85-96 germ plasm resource coloration result；
Fig. 9 utilizes this inventive method to detect 97-108 germ plasm resource coloration result；
Figure 10 utilizes this inventive method to detect 109-120 germ plasm resource coloration result；
Figure 11 utilizes this inventive method to detect 121-132 germ plasm resource coloration result；
Figure 12 utilizes this inventive method to detect 133-144 germ plasm resource coloration result；
Figure 13 utilizes this inventive method to detect 145-156 germ plasm resource coloration result；
Figure 14 utilizes this inventive method to detect 157-168 germ plasm resource coloration result；
Figure 15 utilizes this inventive method to detect 169-173 germ plasm resource coloration result.
Detailed description of the invention
Material: randomly choose 173 parts of wheat breed (strain) resources in seed resource storehouse
Reagent: 1% phenol solution (1g phenol is dissolved in 100ml water)
Utensil: culture dish, filter paper, tweezers, incubator
Method: every part of germ plasm resource takes wheat seed 100, is repeated 3 times, the phenol with 1.0% soaks 15min, takes out
It is placed on (ventral groove is downward) in the culture dish being covered with the filter paper that 1% phenol wet is crossed, culture dish lid covers same phenol wet
The filter paper moistened, builds culture dish, puts in the incubator of 37 DEG C, and dye 25min, observes coloration result.
Standard of perfection: (1) achromophil seed is high whiteness (whiteness value is more than 80) wheat breed seed.
(2) dye light brown or brown for (whiteness value is below 79) low whiteness wheat breed seed.The standard of the method
Really rate is 99%.
Qualification result see table 1,2,3
Table 1
Table 2
Table 3
Result verification: measure 173 parts with digital display whiteness instrument (elegant demeanour instruments and meters company limited, model: SBDY-1 are pleased in Shanghai)
Germ plasm resource Flour whiteness, the reliability of checking this method measurement result.
Method step: take above-mentioned 173 parts of wheat seeds
(1) every part of germ plasm resource weighs wheat seed 200g, uses 130 DEG C to dry 60min and measure moisture after removing impurity.
(2) wheat wetting: after selected wheat cleaning, wheat wetting to Target moisture 15.0%, wheat wetting amount of water be=[(enters
Mill moisture-actual moisture)/(1-water into the mill)] × seed gross weight, then place pulverizing after 24h.
(3) pulverizing: shelling one time, reduction one time, calculate flour extraction.Grind flour to place 1 week in laboratory.
(4) measuring brightness: weigh 7.00g flour.Then Flour whiteness is measured with intelligence whiteness instrument.Measure its whiteness respectively
And record.Qualification result is shown in Table 4,5,6
Table 4
Table 5
Table 6
3. the method application in practice.
(1) high whiteness Wheat Germplasm Resources is screened.High whiteness is carried out little for wheat breeding scientific research institution and seeds company
The screening of wheat germ plasm resource.
(2) flour mill quickly provides the wheat seed of high whiteness in purchase wheat seed, Semen Tritici aestivi flour production process,
During powder joined by flour, can quickly measure Semen Tritici aestivi flour whiteness in this way simultaneously, provide reference for joining powder.Save money
Source, improves efficiency.
(3) for the selection-breeding of R&D institution's high whiteness wheat breed.The improvement of wheat breeding Flour whiteness need to be from genetic breeding
Carry out in-depth study, mainly include the whiteness index measuring variety source on a large scale, screen, collect and to preserve whiteness excellent
Variety source, the high whiteness excellence wheat germplasm filtered out can be used for cultivating in strong gluten flour high whiteness new variety of wheat.Make
In the middle of thing breeding process during the Single-plant selection in crucial epoch, often grain weight is less, can be by the method with minimal amount of
Seed just can identify Semen Tritici aestivi individual plant or the strain of high whiteness.
4. the method advantage and prospect
(1) advantage:
1. principle is reliable, and result is accurate.Phenol staining is the reaction of a kind of pure chemistry, it utilize the steam of phenol solution with
There is chemical reaction in the phenol oxidase in seed coat.The oxidase content of different kind kind Intradermal is different, Flour whiteness
High content is less, and the content that Flour whiteness is low is more.
The most convenient and swift, save time quickly.Required instrument and equipment and article less (incubator, culture dish, filter paper, tweezers) measure
Time only needs 25min.
3. convenient simplicity is measured, it is simple to penetration and promotion.The method is easy to for basic unit's scientific research breeding and little company breeder
Grasping, expense is relatively low simultaneously.It is easy to promote the use of in whole nation scientific research breeding unit.
The most with low cost.Phenol price is 20 yuan of 1 bottle of 500g, at least can use 500 times.Cost is 0.04 yuan every time.
Needed for test determination, instrument article are few simultaneously so that measure whiteness cost of consumption lower.
5. a reasonable Research approaches is provided for the detection of high whiteness flour purity.
6. the advantage in terms of scientific research breeding, the germ plasm resource with whiteness as criteria for classification is poor, is that whiteness genetic improvement exists
Difficulty in practice；The Wheat Germplasm Resources screening high whiteness be can quickly detect in this approach, Flour whiteness height, quality excavated
Excellent wheat germplasm, provides technical support for cultivating High quality glutenous wheat new varieties in high whiteness.And can be few at raw material, the used time
In the case of short, quickly recording the whiteness of all kind wheat flours, the detection for this index of whiteness provides the most convenient.
(2) prospect:
Semen Tritici aestivi flour whiteness is to weigh the important indicator that flower characters is good and bad, is the major criterion dividing flour grade.I
It is 74.8 that state promotes mainly the average Flour whiteness of wheat breed, and the Flour whiteness sold on market commonly reaches more than 80 the most easy quilts
Accreditation.Flour whiteness high (>=80%) of less types, and mostly be the weak-gluten wheat kind of opaque, lack Flour whiteness high in
Muscle and strong gluten wheat variety, for meeting the market competitiveness of people's demand to high whiteness flour and raising milling product, almost
In host country, flour enterprises the most once used benzoyl peroxide class brightening agent to bleach flour, and brightening agent in a lot of flour
Content also severe overweight.Research shows, human body can be caused serious harm by the flour being eaten for a long time benzoyl peroxide bleaching.With
Developing rapidly of China's economy and improving constantly of living standards of the people, people are more and more higher to the cry of pollution-free food.Cause
This, cultivate or produce the pure white new variety of wheat of pink colour and meet consumer's requirement to Flour whiteness.To reduce making of brightening agent
With, the safety and health quality improving flour and flour-made food is extremely urgent.Therefore, during selection-breeding is high with popularization Flour whiteness by force
Muscle Semen Tritici aestivi is the task of top priority.
The method is simple, and instrument is few, low cost, can promote rapidly in national all breeding units, be used for
The high whiteness variety source of the most large-scale mensuration screens, collects and preserve.For then carrying out sexual hybridization or genetic recombination,
The genetic development of research whiteness, in depth research whiteness correlative factor, use tradition sexual hybridization to tie mutually with modern biotechnology
Close, the kind that selection-breeding whiteness is excellent.
Claims (1)
1. the method for a quick detection high whiteness flour wheat seed, it is characterised in that carry out as steps described below:
Every part of germ plasm resource takes wheat seed 100, soaks 15min with the phenol that mass concentration is 1.0%, and taking-up is placed on and is covered with
Mass concentration be the filter paper that 1% phenol wet is crossed culture dish in (ventral groove is downward), on culture dish lid cover same phenol wet
The filter paper moistened, builds culture dish, puts in the incubator of 37 DEG C, and dye 25min, observes coloration result, and said method is repeated 3 times；
Standard of perfection: (1) achromophil seed is the wheat breed seed of high whiteness, i.e. whiteness value wheat breed more than 80
Seed；(2) dye light brown or brown for low whiteness wheat breed seed, i.e. whiteness value wheat breed kind below 79
Son.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872462A (en) * | 2017-04-05 | 2017-06-20 | 河南科技学院 | The developer and application method of quick detection whiteness flour wheat seed resource high |
| CN110095329A (en) * | 2019-06-06 | 2019-08-06 | 山东农业大学 | A kind of colouring method of quick measurement wheat seed purity |
| CN113866176A (en) * | 2021-09-18 | 2021-12-31 | 北京中仪智控科技有限公司 | Full-automatic analyzer for imperfect grains |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1220079A (en) * | 1997-12-19 | 1999-06-23 | 孟宪剑 | Method for treating winter wheat seed |
| CN103424519A (en) * | 2012-05-15 | 2013-12-04 | 青岛天祥食品集团有限公司 | Inspection methods of wheat whiteness and wheat gluten |
| CN104099331A (en) * | 2013-04-12 | 2014-10-15 | 中国科学院遗传与发育生物学研究所 | Method for increasing germination rate of stored wheat and white degree of flour through endosperm specific silencing of expression of wheat lipoxygenase gene |
-
2016
- 2016-07-05 CN CN201610526685.2A patent/CN106198406A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1220079A (en) * | 1997-12-19 | 1999-06-23 | 孟宪剑 | Method for treating winter wheat seed |
| CN103424519A (en) * | 2012-05-15 | 2013-12-04 | 青岛天祥食品集团有限公司 | Inspection methods of wheat whiteness and wheat gluten |
| CN104099331A (en) * | 2013-04-12 | 2014-10-15 | 中国科学院遗传与发育生物学研究所 | Method for increasing germination rate of stored wheat and white degree of flour through endosperm specific silencing of expression of wheat lipoxygenase gene |
Non-Patent Citations (1)
| Title |
|---|
| 杨雪: "冬小麦多酚氧化酶活性选择对面粉白度及抗穗发芽的影响", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872462A (en) * | 2017-04-05 | 2017-06-20 | 河南科技学院 | The developer and application method of quick detection whiteness flour wheat seed resource high |
| CN110095329A (en) * | 2019-06-06 | 2019-08-06 | 山东农业大学 | A kind of colouring method of quick measurement wheat seed purity |
| CN113866176A (en) * | 2021-09-18 | 2021-12-31 | 北京中仪智控科技有限公司 | Full-automatic analyzer for imperfect grains |
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