CN105548138B - The detection method of brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum - Google Patents
The detection method of brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum Download PDFInfo
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- CN105548138B CN105548138B CN201610021401.4A CN201610021401A CN105548138B CN 105548138 B CN105548138 B CN 105548138B CN 201610021401 A CN201610021401 A CN 201610021401A CN 105548138 B CN105548138 B CN 105548138B
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- yeast activity
- sample cell
- raman spectrum
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- zymotic fluid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/14—Beverages
- G01N33/146—Beverages containing alcohol
Abstract
The invention discloses the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum, including pump, sample cell, triple valve, flow cell, laser, optical fiber, Raman probe, speculum, spectrometer, computer；The laser is connected to Raman probe by optical fiber, and the head of the Raman probe is connected with the first surface of sample cell, and the speculum is arranged on the second surface of the sample cell, and the first surface is parallel to each other with second surface；The spectrometer is connected to computer by communication interface；The sample cell is made of clear material.The invention also discloses the brewing fermentation liquid yeast activity online test method based on Raman spectrum.Zymotic fluid yeast activity carries out on-line checking during the present invention realizes wine brewing, and to instructing winery optimization fermentation process to have great significance, the present invention can accurately and rapidly measure zymotic fluid yeast activity.
Description
Technical field
The present invention relates to the Detection ＆ Controling field of brewing fermentation process, the more particularly to brewing fermentation based on Raman spectrum
Liquid yeast activity on-line measuring device and method.
Background technology
Fermentation is one of mostly important, most critical link during making wine, and the quality of zymotic fluid yeast quality is directly closed
Be to its tunning yield is how many and quality.The quality of yeast is generally evaluated with activity and vigor, and activity refers to
The viability of cell, yeast activity refer to the physiological status of yeast cells, also are understood as adaptation energy of the living cells to environment
Power and metabolic capability.Yeast activity is the yeast of vigor only stablize, high an important factor for influencing fermenting property and taste,
The wine of high quality can just be brewed.Therefore, how to realize the on-line checking to zymotic fluid yeast activity, be always that liquor industry closes
The heart and the emphasis of research.
Traditional yeast activity measure mainly has blood counting chamber cultivation, vigor titration, acidifying amylograph, oxygen
Absorption process, CO2Method for releasing, magnesium ion method for releasing, methylenum careuleum reduction method etc..But these methods are all there are some defects and not
Foot, as sensitivity is low；Most assay methods are off-line measurement, some miscellaneous bacterias may be introduced in measurement process, influence measurement knot
Fruit；Detection time is longer, causes obtained feedback information to relatively lag behind, it is impossible to reflect the change of yeast activity in time, from
And influence the real time monitoring to fermentation process；Sometimes also need to add some fluorescent dyes, be highly detrimental to production process
Line detects.
In brewing fermentation field, due to the complexity and diversity of fermentation broth contents, develop at present for zymotic fluid
The device of composition detection is considerably less, without a kind of device can realize to during brewing fermentation zymotic fluid yeast activity it is online
Detection.
Raman spectrum has quick, efficient, lossless point as a kind of spectral detection means received significant attention in recent years
Analysis, without pre-treatment, it is pollution-free the advantages that, be successfully applied to petrochemical industry, medicine, pharmacy, environmental protection, archaeology mirror
The fields such as fixed, food security.But this method is all not applied to the quantitative analysis of yeast activity so far, more without for making wine
During zymotic fluid yeast activity on-line checking.
The content of the invention
In order to overcome the disadvantages mentioned above of the prior art and deficiency, it is an object of the invention to provide one kind to be based on Raman spectrum
Brewing fermentation liquid yeast activity on-line measuring device, can to during wine brewing zymotic fluid yeast activity carry out on-line checking, can
Accurately and rapidly measure zymotic fluid yeast activity.
Another object of the present invention is to provide the brewing fermentation liquid yeast activity on-line checking based on above-mentioned Raman spectrum
The brewing fermentation liquid yeast activity online test method based on Raman spectrum of device.
The purpose of the present invention is achieved through the following technical solutions：
Brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum, including pump, sample cell, triple valve, stream
Logical pond, laser, optical fiber, Raman probe, speculum, spectrometer, computer；
The arrival end of the pump is connected with the sample tap of fermentation tank, and the port of export of pump is connected with the injection port of sample cell；Institute
The outlet of sample cell is stated with the import of triple valve to be connected, two of triple valve outlets respectively with fermentation tank and flow cell phase
Even；
The laser is connected to Raman probe, the head of the Raman probe and the first surface of sample cell by optical fiber
It is connected, the speculum is arranged on the second surface of the sample cell, and the first surface is parallel to each other with second surface；Institute
State spectrometer and computer is connected to by communication interface；The sample cell is made of clear material；
The Raman probe is connected by optical fiber with spectrometer.
The brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum, further includes display；It is described
Computer is connected to display by communication port.
The laser is visible laser or infrared light laser.
The Raman probe is the Raman probe of focus adjustable.
The sample cell is made of quartz material.
Brewing fermentation liquid yeast activity online test method based on Raman spectrum, comprises the following steps：
(1) pump is opened, triple valve leads to flow cell, and zymotic fluid to be measured is introduced sample cell；
(2) after sample cell gradually fills zymotic fluid, laser is opened, the Raman light of zymotic fluid is gathered by Raman probe
Modal data；After denoising, smooth, baseline correction are carried out to Raman spectrum data, based on successive projection algorithm extraction nucleic acid, protein
With the characteristic spectrum of lipid；
(3) zymotic fluid in sample cell circulates pond by triple valve, and training sample is collected in flow cell, uses vigor
Zymotic fluid yeast activity in the method measure flow cell of titration；
(4) using the intensity of nucleic acid, protein and lipid profile peak as independent variable, with the fermentation of the method measure of vigor titration
Liquid yeast activity is dependent variable, and yeast activity and nucleic acid, protein and lipid profile peak intensity are established with chemometrics method
Quantitative Analysis Model；
(5) triple valve leads to fermentation tank, and zymotic fluid circulates between fermentation tank and sample cell, passes through Raman probe
The Raman spectrum data of collection zymotic fluid in real time, after denoising, smooth, baseline correction are carried out to Raman spectrum data, based on continuous
The characteristic spectrum of projection algorithm extraction nucleic acid, protein and lipid；
(6) quantitative analysis of the characteristic spectrum for nucleic acid, protein and the lipid for obtaining step (5) described in step (4)
Model prediction yeast activity.
The brewing fermentation liquid yeast activity online test method based on Raman spectrum, carry out after step (6) with
Lower step：
(7) prediction result of step (6) is shown over the display.
The principle of the present invention is：
The basis of Raman spectrum quantitative analysis is that there are certain ratio pass for raman scattering intensity and its concentration of test analyte
System.And the change of zymotic fluid yeast activity and its nucleic acid, protein and the relevant characteristic peak of lipid during brewing fermentation
Change directly related.Therefore, quantitative analysis can be established with reference to chemometrics method by Raman spectrum on-line measuring device
Model, realizes the on-line checking to zymotic fluid yeast activity during wine brewing.
Compared with prior art, the present invention has the following advantages and beneficial effect：
(1) the device of the invention be it is closed, circulation, so not only can to avoid pollution of other microorganisms to yeast, but also
Can be to avoid the wasting of resources (in addition to training sample gathers).
(2) present invention can replace conventional offline detection method, realize the on-line checking of zymotic fluid yeast activity.
(3) test process of the invention need not destroy sample, contact sample, it is not necessary to add any fluorescent dye, belong to
In " green " analytical technology.
(4) the device of the invention is compact-sized, small, and device is applicable not only to brewing industry production, is also applied for reality
Test room small-sized fermentation experiment.
(5) the device of the invention is safeguarded fairly simple, can be with long time continuous working.
(6) in the actual production process, training sample can be constantly updated, to ensure the accuracy of calibration model and sane
Property.
Brief description of the drawings
Fig. 1 is the schematic diagram of the brewing fermentation liquid yeast activity on-line measuring device of the invention based on Raman spectrum.
Fig. 2 is the flow chart of the brewing fermentation liquid yeast activity online test method of the invention based on Raman spectrum.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment
The brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum of the present embodiment, including pump 3, sample cell
4th, triple valve 5, flow cell 6, laser 7, optical fiber 8, Raman probe 9, speculum 10, spectrometer 11, computer 12 and display
13。
The arrival end of the pump 3 is connected with fermentation tank 1 (built-in zymotic fluid 2) sample tap, pumps 3 port of export and sample cell 4
Injection port be connected, the outlet of the sample cell 4 is connected with the import of triple valve 5, two of the triple valve 5 outlets respectively with
Fermentation tank 1 is connected with flow cell 6, and the flow cell 6 is used for placing the zymotic fluid for treating conventional method measurement or is detained for discharging
In the zymotic fluid of sample cell；
The laser 7 is connected by optical fiber 8 with Raman probe 9, and the of the head of the Raman probe 9 and sample cell 4
One surface is connected, and the speculum 10 is arranged on the second surface of the sample cell 4, the first surface and second surface phase
It is mutually parallel；Sample cell 4 is used for placing the zymotic fluid for treating Raman spectroscopy scans；
The Raman probe 9 is connected to spectrometer 11 by another optical fiber 8, and the spectrometer 11 is connected by communication interface
Computer 12 is connected to, the computer 12 is connected to display 13 by communication port, and the display 13 is used for zymotic fluid ferment
The display of female activity level.
In the present embodiment, laser is semiconductor laser, and output light is visible or infrared light.The Raman probe
Focal length can be adjusted, and ensure that light beam can accurately focus on measured zone during measurement；The display is light-emitting diode display.
The brewing fermentation liquid yeast activity online test method based on Raman spectrum of the present embodiment, comprises the following steps：
(1) pump is opened, triple valve leads to flow cell, and zymotic fluid to be measured slowly introduces sample cell；
(2) after sample cell gradually fills zymotic fluid, laser is opened, the Raman light of zymotic fluid is gathered by Raman probe
Modal data, carries out Raman spectrum initial data the pretreatments such as denoising, smooth, baseline correction by software WIRE 3.3, is based on
Successive projection algorithm extracts and nucleic acid, protein and the relevant characteristic spectrum of lipid；
(3) zymotic fluid in sample cell circulates pond by triple valve, and a certain number of training are collected in flow cell
Sample, the method titrated with vigor measure zymotic fluid yeast activity in flow cell；
(4) using the intensity of nucleic acid, protein and lipid profile peak as independent variable, with the fermentation of the method measure of vigor titration
Liquid yeast activity is dependent variable, and yeast activity and nucleic acid, protein and lipid profile peak intensity are established with chemometrics method
Quantitative Analysis Model；
(5) triple valve leads to fermentation tank, and zymotic fluid circulates between fermentation tank and sample cell, passes through Raman probe
The Raman spectrum data of collection zymotic fluid in real time, after denoising, smooth, baseline correction are carried out to Raman spectrum data, based on continuous
The characteristic spectrum of projection algorithm extraction nucleic acid, protein and lipid；
(6) quantitative analysis of the characteristic spectrum for nucleic acid, protein and the lipid for obtaining step (5) described in step (4)
Model prediction yeast activity；
(7) prediction result of step (6) is shown over the display.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from the embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (6)
1. the detection method of the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum, it is characterised in that
The brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum includes pump, sample cell, triple valve, stream
Logical pond, laser, optical fiber, Raman probe, speculum, spectrometer, computer；
The arrival end of the pump is connected with the sample tap of fermentation tank, and the port of export of pump is connected with the injection port of sample cell；The sample
The outlet in product pond is connected with the import of triple valve, and two outlets of the triple valve are connected with fermentation tank and flow cell respectively；
The laser is connected to Raman probe by optical fiber, and the head of the Raman probe is connected with the first surface of sample cell
Connect, the speculum is arranged on the second surface of the sample cell, and the first surface is parallel to each other with second surface；The light
Spectrometer is connected to computer by communication interface；The sample cell is made of clear material；
The Raman probe is connected by optical fiber with spectrometer；
Comprise the following steps：
(1) pump is opened, triple valve leads to flow cell, and zymotic fluid to be measured is introduced sample cell；
(2) after sample cell gradually fills zymotic fluid, laser is opened, the Raman spectrum number of zymotic fluid is gathered by Raman probe
According to；After denoising, smooth, baseline correction are carried out to Raman spectrum data, based on successive projection algorithm extraction nucleic acid, protein and fat
The characteristic spectrum of class；
(3) zymotic fluid in sample cell circulates pond by triple valve, and training sample is collected in flow cell, is titrated with vigor
Method measure flow cell in zymotic fluid yeast activity；
(4) using the intensity of nucleic acid, protein and lipid profile peak as independent variable, with the zymotic fluid ferment of the method measure of vigor titration
Female vigor is dependent variable, and determining for yeast activity and nucleic acid, protein and lipid profile peak intensity is established with chemometrics method
Measure analysis model；
(5) triple valve leads to fermentation tank, and zymotic fluid circulates between fermentation tank and sample cell, real-time by Raman probe
The Raman spectrum data of zymotic fluid is gathered, after denoising, smooth, baseline correction are carried out to Raman spectrum data, based on successive projection
The characteristic spectrum of algorithm extraction nucleic acid, protein and lipid；
(6) Quantitative Analysis Model of the characteristic spectrum for nucleic acid, protein and the lipid for obtaining step (5) described in step (4)
Predict yeast activity.
2. the detection side of the brewing fermentation liquid yeast activity on-line measuring device according to claim 1 based on Raman spectrum
Method, it is characterised in that followed the steps below after step (6)：
(7) prediction result of step (6) is shown over the display.
3. the detection side of the brewing fermentation liquid yeast activity on-line measuring device according to claim 1 based on Raman spectrum
Method, it is characterised in that further include display；The computer is connected to display by communication port.
4. the detection side of the brewing fermentation liquid yeast activity on-line measuring device according to claim 1 based on Raman spectrum
Method, it is characterised in that the laser is visible laser or infrared light laser.
5. the detection side of the brewing fermentation liquid yeast activity on-line measuring device according to claim 1 based on Raman spectrum
Method, it is characterised in that the Raman probe is the Raman probe of focus adjustable.
6. the detection side of the brewing fermentation liquid yeast activity on-line measuring device according to claim 1 based on Raman spectrum
Method, it is characterised in that the sample cell is made of quartz material.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3865861A1 (en) * | 2020-02-13 | 2021-08-18 | Kaiser Optical Systems Inc. | Real-time monitoring of wine fermentation properties using raman spectroscopy |
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| CN106932378A (en) * | 2017-03-29 | 2017-07-07 | 浙江大学 | The on-line detecting system and method for a kind of sour gas composition based on Raman spectrum |
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| CN107561058B (en) * | 2017-09-12 | 2020-11-17 | 青岛啤酒股份有限公司 | Method for evaluating metabolic similarity of flavor substances of industrial Lager yeast strains |
| CN110079446B (en) * | 2019-02-28 | 2022-05-17 | 保定蕴生生物科技有限公司 | Fermentation tank for on-line monitoring of microbial fermentation products |
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| CN111141724A (en) * | 2020-03-19 | 2020-05-12 | 茅台学院 | On-line detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum |
| CN112730376A (en) * | 2020-12-17 | 2021-04-30 | 山东省科学院生物研究所 | Device for detecting polysaccharide in fermentation liquor based on Raman spectrum, method and application thereof |
| CN112903662A (en) * | 2021-04-13 | 2021-06-04 | 南京信息工程大学 | Portable laser Raman spectrum detection device for liquid phase real-time detection |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104020150A (en) * | 2014-03-19 | 2014-09-03 | 苏州久王环保科技有限公司 | Device and method for measuring concentration of ammonium salt based on laser Raman spectrum technology |
| CN203824912U (en) * | 2014-03-19 | 2014-09-10 | 苏州久王环保科技有限公司 | Ammonium salt concentration measuring device based on laser-Raman spectrum technology |
| CN205333518U (en) * | 2016-01-13 | 2016-06-22 | 华南理工大学 | Making wine zymotic fluid yeast vigor on -line measuring device based on raman spectroscopy |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030030800A1 (en) * | 2001-07-15 | 2003-02-13 | Golden Josh H. | Method and system for the determination of arsenic in aqueous media |
-
2016
- 2016-01-13 CN CN201610021401.4A patent/CN105548138B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104020150A (en) * | 2014-03-19 | 2014-09-03 | 苏州久王环保科技有限公司 | Device and method for measuring concentration of ammonium salt based on laser Raman spectrum technology |
| CN203824912U (en) * | 2014-03-19 | 2014-09-10 | 苏州久王环保科技有限公司 | Ammonium salt concentration measuring device based on laser-Raman spectrum technology |
| CN205333518U (en) * | 2016-01-13 | 2016-06-22 | 华南理工大学 | Making wine zymotic fluid yeast vigor on -line measuring device based on raman spectroscopy |
Non-Patent Citations (2)
| Title |
|---|
| Raman Spectroscopy and Chemometrics for On-Line Control of Glucose Fermentation by Saccharomyces cerevisiae;Thiago C. A ´ vila et al.;《Biotechnol. Prog.》;20120921;第28卷(第6期);摘要、第1599页右栏倒数第1段-1600页左栏第1段及图2 * |
| 活力滴定法评价啤酒酵母活力的探讨;孙军勇等;《啤酒科技》;20110228(第2期);第16-18页 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3865861A1 (en) * | 2020-02-13 | 2021-08-18 | Kaiser Optical Systems Inc. | Real-time monitoring of wine fermentation properties using raman spectroscopy |
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