CN105548138A - On-line wine making fermentation liquid yeast activity detection device and method based on Raman spectrum - Google Patents
On-line wine making fermentation liquid yeast activity detection device and method based on Raman spectrum Download PDFInfo
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- CN105548138A CN105548138A CN201610021401.4A CN201610021401A CN105548138A CN 105548138 A CN105548138 A CN 105548138A CN 201610021401 A CN201610021401 A CN 201610021401A CN 105548138 A CN105548138 A CN 105548138A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
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- G—PHYSICS
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Abstract
The invention discloses an on-line wine making fermentation liquid yeast activity detection device based on a Raman spectrum. The device comprises a pump, a sample pool, a three-way valve, a communication pool, a laser, an optical fiber, a Raman probe, a reflecting mirror and a computer; the laser is connected to the Raman probe through the optical fiber, the head of the Raman probe is connected with the first surface of the sample pool, the reflecting mirror is arranged on the second surface of the sample pool, and the first surface and the second surface are parallel to each other; a spectrometer is connected to the computer through a communication interface; the sample pool is made of a transparent material. The invention further discloses an on-line wine making fermentation liquid yeast activity detection method based on the Raman spectrum. According to the device and method, on-line wine making fermentation liquid yeast activity detection is achieved in the wine making process, the important significance on guiding a brewery to optimize the fermentation process is achieved, and the fermentation liquid yeast activity can be accurately and quickly detected.
Description
Technical field
The present invention relates to the Detection & Controling field of brewing fermentation process, particularly based on brewing fermentation liquid yeast activity on-line measuring device and the method for Raman spectrum.
Background technology
Fermentation is one of link of the most important in wine brewing process, most critical, and the quality of fermentation liquor yeast quality is directly connected to the how many and quality of the output of its tunning.The quality of yeast is generally evaluated with active and vigor, and activity is the viability of phalangeal cell, and yeast activity refers to the physiological status of yeast cells, also can be regarded as living cells to the adaptive faculty of environment and metabolic capability.Yeast activity is the key factor affecting fermenting property and taste, only has yeast that is stable, high vigor, just can brew high-quality wine.Therefore, how to realize the on-line checkingi to fermentation liquor yeast activity, be the emphasis that liquor industry is concerned about and is studied always.
Traditional yeast activity measures and mainly contains blood counting chamber cultivation, vigor titrimetry, acidifying amylograph, the absorption process of oxygen, CO
2method for releasing, magnesium ion method for releasing, methylenum careuleum reducing process etc.But these methods all exist some defects and deficiency, as sensitivity is low; Most assay method is off-line measurement, may introduce some miscellaneous bacterias, affect measurement result in measuring process; Detection time is longer, causes obtained feedback information to relatively lag behind, and can not reflect the change of yeast activity in time, thus the real-time monitoring of impact to sweat; Sometimes also need to add some fluorescent dyes, be unfavorable for very much the on-line checkingi of production run.
In brewing fermentation field, due to the complexity and diversity of fermentation broth contents, develop the device detected for fermentation broth contents at present considerably less, there is no the on-line checkingi that a kind of device can realize fermentation liquor yeast activity in brewing fermentation process.
Raman spectrum is as a kind of spectral detection means being subject to extensive concern in recent years; have fast, efficient, nondestructive analysis, without the need to pre-treatment, the advantage such as pollution-free, be successfully applied to the fields such as petrochemical complex, medical science, pharmacy, environmental protection, archaeology qualification, food security.But this method is not all applied to the quantitative test of yeast activity so far, more not used for the on-line checkingi of fermentation liquor yeast activity in wine brewing process.
Summary of the invention
In order to overcome the above-mentioned shortcoming of prior art with not enough, the object of the present invention is to provide a kind of brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum, on-line checkingi can be carried out to fermentation liquor yeast activity in wine brewing process, fermentation liquor yeast activity can be measured accurately and rapidly.
The brewing fermentation liquid yeast activity online test method based on Raman spectrum of the brewing fermentation liquid yeast activity on-line measuring device provided based on above-mentioned Raman spectrum is provided.
Object of the present invention is achieved through the following technical solutions:
Based on the brewing fermentation liquid yeast activity on-line measuring device of Raman spectrum, comprise pump, sample cell, T-valve, flow cell, laser instrument, optical fiber, Raman probe, catoptron, spectrometer, computing machine;
The inlet end of described pump is connected with the sample tap of fermentation tank, and delivery side of pump end is connected with the injection port of sample cell; The outlet of described sample cell is connected with the import of T-valve, and two outlets of described T-valve are connected with flow cell with fermentation tank respectively;
Described laser instrument is by Fiber connection in Raman probe, and the head of described Raman probe is connected with the first surface of sample cell, and described catoptron is located on the second surface of described sample cell, and described first surface and second surface are parallel to each other; Described spectrometer is connected to computing machine by communication interface; Described sample cell is made up of transparent material;
Described Raman probe is connected with spectrometer by optical fiber.
The described brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum, also comprises display; Described computing machine is connected to display by communication port.
Described laser instrument is visible laser or infrared light laser instrument.
Described Raman probe is the Raman probe of focus adjustable.
Described sample cell is made up of quartz material.
Based on the brewing fermentation liquid yeast activity online test method of Raman spectrum, comprise the following steps:
(1) open pump, T-valve leads to flow cell, and fermentation liquor to be measured is introduced sample cell;
(2) after sample cell fills fermentation liquor gradually, open laser instrument, gathered the Raman spectrum data of fermentation liquor by Raman probe; After denoising, level and smooth, baseline correction are carried out to Raman spectrum data, extract the characteristic spectrum of nucleic acid, protein and lipid based on successive projection algorithm;
(3) fermentation liquor in sample cell to circulate pond through T-valve, in flow cell, collect training sample, measures fermentation liquor yeast activity in flow cell by the method for vigor titration;
(4) with the intensity at nucleic acid, protein and lipid profile peak for independent variable, the fermentation liquor yeast activity measured with the method for vigor titration, for dependent variable, sets up the Quantitative Analysis Model of yeast activity and nucleic acid, protein and lipid profile peak intensity with chemometrics method;
(5) T-valve leads to fermentation tank, fermentation liquor circulates between fermentation tank and sample cell, by the Raman spectrum data of Raman probe Real-time Collection fermentation liquor, after denoising, level and smooth, baseline correction are carried out to Raman spectrum data, extract the characteristic spectrum of nucleic acid, protein and lipid based on successive projection algorithm;
(6) the Quantitative Analysis Model prediction yeast activity described in the characteristic spectrum step (4) of the nucleic acid, protein and the lipid that step (5) are obtained.
The described brewing fermentation liquid yeast activity online test method based on Raman spectrum, carry out following steps after step (6):
(7) predicting the outcome of step (6) is shown over the display.
Principle of the present invention is:
The basis of Raman spectrum quantitative test is that the raman scattering intensity of test analyte and its concentration exist certain proportionate relationship.And the change of the characteristic peak that the change of fermentation liquor yeast activity is relevant to its nucleic acid, protein and lipid is directly related in brewing fermentation process.Therefore, by Raman spectrum on-line measuring device, Quantitative Analysis Model can be set up in conjunction with chemometrics method, realize the on-line checkingi to fermentation liquor yeast activity in wine brewing process.
Compared with prior art, the present invention has the following advantages and beneficial effect:
(1) device of the present invention is airtight, circulation, other microorganisms so both can have been avoided the pollution of yeast, can avoid again the wasting of resources (except training sample collection).
(2) the present invention can replace conventional offline detection method, realizes the on-line checkingi of fermentation liquor yeast activity.
(3) test process of the present invention does not need to destroy sample, contact sample, does not need to add any fluorescent dye, belongs to " green " analytical technology.
(4) apparatus structure of the present invention is compact, and volume is little, and device is not only applicable to brewing industry and produces, and is applicable to laboratory room small-sized fermenting experiment yet.
(5) device of the present invention is safeguarded fairly simple, can long time continuous working.
(6) in actual production process, training sample can be constantly updated, to guarantee accuracy and the robustness of calibration model.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the brewing fermentation liquid yeast activity on-line measuring device that the present invention is based on Raman spectrum.
Fig. 2 is the process flow diagram of the brewing fermentation liquid yeast activity online test method that the present invention is based on Raman spectrum.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment
The brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum of the present embodiment, comprises pump 3, sample cell 4, T-valve 5, flow cell 6, laser instrument 7, optical fiber 8, Raman probe 9, catoptron 10, spectrometer 11, computing machine 12 and display 13.
The inlet end of described pump 3 is connected with fermentation tank 1 (in-built fermentation liquor 2) sample tap, the endpiece of pump 3 is connected with the injection port of sample cell 4, the outlet of described sample cell 4 is connected with the import of T-valve 5, two outlets of described T-valve 5 are connected with flow cell 6 with fermentation tank 1 respectively, and described flow cell 6 is used for placing the fermentation liquor for the treatment of that conventional method is measured or is used for discharging the fermentation liquor being trapped in sample cell;
Described laser instrument 7 is connected with Raman probe 9 by optical fiber 8, and the head of described Raman probe 9 is connected with the first surface of sample cell 4, and described catoptron 10 is located on the second surface of described sample cell 4, and described first surface and second surface are parallel to each other; Sample cell 4 is used for placing the fermentation liquor treating Raman spectroscopy scans;
Described Raman probe 9 is connected to spectrometer 11 by another optical fiber 8, described spectrometer 11 is connected to computing machine 12 by communication interface, described computing machine 12 is connected to display 13 by communication port, and described display 13 is for the display of fermentation liquor yeast activity level.
In the present embodiment, laser instrument is semiconductor laser, and output light is visible or infrared light.The focal length of described Raman probe can regulate, and when ensureing to measure, light beam accurately can focus on measured zone; Described display is light-emitting diode display.
The brewing fermentation liquid yeast activity online test method based on Raman spectrum of the present embodiment, comprises the following steps:
(1) open pump, T-valve leads to flow cell, and fermentation liquor to be measured slowly introduces sample cell;
(2) after sample cell fills fermentation liquor gradually, open laser instrument, the Raman spectrum data of fermentation liquor is gathered by Raman probe, by software WIRE3.3, the pre-service such as denoising, level and smooth, baseline correction are carried out to Raman spectrum raw data, extract the characteristic spectrum relevant to nucleic acid, protein and lipid based on successive projection algorithm;
(3) fermentation liquor in sample cell to circulate pond through T-valve, collects the training sample of some in flow cell, measures fermentation liquor yeast activity in flow cell by the method for vigor titration;
(4) with the intensity at nucleic acid, protein and lipid profile peak for independent variable, the fermentation liquor yeast activity measured with the method for vigor titration, for dependent variable, sets up the Quantitative Analysis Model of yeast activity and nucleic acid, protein and lipid profile peak intensity with chemometrics method;
(5) T-valve leads to fermentation tank, fermentation liquor circulates between fermentation tank and sample cell, by the Raman spectrum data of Raman probe Real-time Collection fermentation liquor, after denoising, level and smooth, baseline correction are carried out to Raman spectrum data, extract the characteristic spectrum of nucleic acid, protein and lipid based on successive projection algorithm;
(6) the Quantitative Analysis Model prediction yeast activity described in the characteristic spectrum step (4) of the nucleic acid, protein and the lipid that step (5) are obtained;
(7) predicting the outcome of step (6) is shown over the display.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not limited by the examples; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1., based on the brewing fermentation liquid yeast activity on-line measuring device of Raman spectrum, it is characterized in that, comprise pump, sample cell, T-valve, flow cell, laser instrument, optical fiber, Raman probe, catoptron, spectrometer, computing machine;
The inlet end of described pump is connected with the sample tap of fermentation tank, and delivery side of pump end is connected with the injection port of sample cell; The outlet of described sample cell is connected with the import of T-valve, and two outlets of described T-valve are connected with flow cell with fermentation tank respectively;
Described laser instrument is by Fiber connection in Raman probe, and the head of described Raman probe is connected with the first surface of sample cell, and described catoptron is located on the second surface of described sample cell, and described first surface and second surface are parallel to each other; Described spectrometer is connected to computing machine by communication interface; Described sample cell is made up of transparent material;
Described Raman probe is connected with spectrometer by optical fiber.
2. the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum according to claim 1, is characterized in that, also comprise display; Described computing machine is connected to display by communication port.
3. the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum according to claim 1, it is characterized in that, described laser instrument is visible laser or infrared light laser instrument.
4. the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum according to claim 1, it is characterized in that, described Raman probe is the Raman probe of focus adjustable.
5. the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum according to claim 4, it is characterized in that, described sample cell is made up of quartz material.
6., based on the brewing fermentation liquid yeast activity online test method based on Raman spectrum of the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum described in any one of Claims 1 to 5, it is characterized in that, comprise the following steps:
(1) open pump, T-valve leads to flow cell, and fermentation liquor to be measured is introduced sample cell;
(2) after sample cell fills fermentation liquor gradually, open laser instrument, gathered the Raman spectrum data of fermentation liquor by Raman probe; After denoising, level and smooth, baseline correction are carried out to Raman spectrum data, extract the characteristic spectrum of nucleic acid, protein and lipid based on successive projection algorithm;
(3) fermentation liquor in sample cell to circulate pond through T-valve, in flow cell, collect training sample, measures fermentation liquor yeast activity in flow cell by the method for vigor titration;
(4) with the intensity at nucleic acid, protein and lipid profile peak for independent variable, the fermentation liquor yeast activity measured with the method for vigor titration, for dependent variable, sets up the Quantitative Analysis Model of yeast activity and nucleic acid, protein and lipid profile peak intensity with chemometrics method;
(5) T-valve leads to fermentation tank, fermentation liquor circulates between fermentation tank and sample cell, by the Raman spectrum data of Raman probe Real-time Collection fermentation liquor, after denoising, level and smooth, baseline correction are carried out to Raman spectrum data, extract the characteristic spectrum of nucleic acid, protein and lipid based on successive projection algorithm;
(6) the Quantitative Analysis Model prediction yeast activity described in the characteristic spectrum step (4) of the nucleic acid, protein and the lipid that step (5) are obtained.
7. the brewing fermentation liquid yeast activity online test method based on Raman spectrum according to claim 6, is characterized in that, carries out following steps after step (6):
(7) predicting the outcome of step (6) is shown over the display.
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Cited By (12)
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| CN106932378A (en) * | 2017-03-29 | 2017-07-07 | 浙江大学 | The on-line detecting system and method for a kind of sour gas composition based on Raman spectrum |
| CN106967785A (en) * | 2017-02-27 | 2017-07-21 | 江苏科技大学 | A kind of enzymatic isolation method produces the method for real-time monitoring of blood sugar reducing peptide process |
| CN107389657A (en) * | 2017-08-15 | 2017-11-24 | 江西农业大学 | Antiform oleic acid detection method of content and device in a kind of edible oil |
| CN107561058A (en) * | 2017-09-12 | 2018-01-09 | 青岛啤酒股份有限公司 | A kind of industrial Lager yeast strains flavor substance metabolism method for evaluating similarity |
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| CN110176165A (en) * | 2019-06-20 | 2019-08-27 | 中国石油大学(华东) | A kind of thermal simulation synthesis experiment method of " hydrocarbon-water-rock " interaction |
| CN110865065A (en) * | 2019-11-01 | 2020-03-06 | 季华实验室 | Real-time sampling analysis device adopting Raman spectrum analysis |
| CN111141724A (en) * | 2020-03-19 | 2020-05-12 | 茅台学院 | On-line detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum |
| CN112730376A (en) * | 2020-12-17 | 2021-04-30 | 山东省科学院生物研究所 | Device for detecting polysaccharide in fermentation liquor based on Raman spectrum, method and application thereof |
| CN112903662A (en) * | 2021-04-13 | 2021-06-04 | 南京信息工程大学 | Portable laser Raman spectrum detection device for liquid phase real-time detection |
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| CN105950336A (en) * | 2016-05-10 | 2016-09-21 | 朱兰英 | Glutinous rice wine and rapid determination method for properties of yeast microbes in glutinous rice wine |
| CN106967785A (en) * | 2017-02-27 | 2017-07-21 | 江苏科技大学 | A kind of enzymatic isolation method produces the method for real-time monitoring of blood sugar reducing peptide process |
| CN106967785B (en) * | 2017-02-27 | 2020-09-18 | 江苏科技大学 | Real-time monitoring method for process of producing hypoglycemic peptide by enzymolysis method |
| CN106932378A (en) * | 2017-03-29 | 2017-07-07 | 浙江大学 | The on-line detecting system and method for a kind of sour gas composition based on Raman spectrum |
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| CN110079446A (en) * | 2019-02-28 | 2019-08-02 | 保定蕴生生物科技有限公司 | Microbial fermentation product monitors fermentor on-line |
| CN110079446B (en) * | 2019-02-28 | 2022-05-17 | 保定蕴生生物科技有限公司 | Fermentation tank for on-line monitoring of microbial fermentation products |
| CN110176165B (en) * | 2019-06-20 | 2022-02-15 | 中国石油大学(华东) | Thermal simulation comprehensive experiment method for hydrocarbon-water-rock interaction |
| CN110176165A (en) * | 2019-06-20 | 2019-08-27 | 中国石油大学(华东) | A kind of thermal simulation synthesis experiment method of " hydrocarbon-water-rock " interaction |
| CN110865065A (en) * | 2019-11-01 | 2020-03-06 | 季华实验室 | Real-time sampling analysis device adopting Raman spectrum analysis |
| CN110865065B (en) * | 2019-11-01 | 2023-05-16 | 季华实验室 | Real-time sampling analysis device adopting Raman spectrum analysis |
| CN113252634A (en) * | 2020-02-13 | 2021-08-13 | 凯塞光学系统股份有限公司 | Real-time monitoring of wine fermentation characteristics using raman spectroscopy |
| US11231324B2 (en) | 2020-02-13 | 2022-01-25 | Kaiser Optical Systems Inc. | Real-time monitoring of wine fermentation properties using Raman spectroscopy |
| CN111141724A (en) * | 2020-03-19 | 2020-05-12 | 茅台学院 | On-line detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum |
| CN112730376A (en) * | 2020-12-17 | 2021-04-30 | 山东省科学院生物研究所 | Device for detecting polysaccharide in fermentation liquor based on Raman spectrum, method and application thereof |
| WO2022127203A1 (en) * | 2020-12-17 | 2022-06-23 | 山东省科学院生物研究所 | Apparatus and method for measuring polysaccharides in fermentation broth on basis of raman spectra, and application of apparatus and method |
| CN112903662A (en) * | 2021-04-13 | 2021-06-04 | 南京信息工程大学 | Portable laser Raman spectrum detection device for liquid phase real-time detection |
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