CN111141724A - On-line detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum - Google Patents
On-line detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum Download PDFInfo
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- CN111141724A CN111141724A CN202010194513.6A CN202010194513A CN111141724A CN 111141724 A CN111141724 A CN 111141724A CN 202010194513 A CN202010194513 A CN 202010194513A CN 111141724 A CN111141724 A CN 111141724A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The invention discloses a saccharomyces cerevisiae fermentation broth yeast activity online detection device based on Raman spectrum, relates to the technical field of brewing, solves the problem of low detection accuracy of the Raman spectrum detection device in the prior art, and has the technical key points that: the device comprises a first water pump, a three-way valve is connected to the first water pump through a water pipe, the three-way valve is connected to a detection box through a water pipe, the detection box is arranged on a spectrum detection device, a filtering device used for filtering wine liquid is further arranged between the first water pump and the three-way valve, the three-way valve is further connected with a second water pump, the second water pump is connected to a clean water pool through a water pipe, the three-way valve is an electromagnetic valve, and a channel between the filtering device and a channel between the second water pump and the detection box is; the invention can improve the accuracy of the measurement result.
Description
Technical Field
The invention relates to the technical field of brewing, in particular to a device for detecting the activity of saccharomyces cerevisiae fermentation liquor yeast on line based on Raman spectrum.
Background
Fermentation is one of the most important and critical links in the brewing process, and the quality of the fermentation broth yeast is directly related to the yield and quality of the fermentation product. The quality of yeast is generally evaluated by activity and viability, wherein activity refers to the viability of the cells, and yeast viability refers to the physiological state of the yeast cells, and can also be understood as the adaptability and metabolic capability of the living cells to the environment. The yeast activity is an important factor influencing the fermentation performance and taste, and high-quality wine can be brewed only by stable and high-activity yeast. Therefore, how to realize online detection of the activity of the fermentation broth yeast is always the key point of concern and research in the brewing industry.
Chinese patent publication No. CN105548138B discloses an online detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum, which comprises a pump, a sample cell, a three-way valve, a flow cell, a laser, an optical fiber, a Raman probe, a reflector, a spectrometer and a computer; the laser is connected with the Raman probe through an optical fiber, the head of the Raman probe is connected with the first surface of the sample cell, the reflector is arranged on the second surface of the sample cell, and the first surface and the second surface are parallel to each other; the spectrometer is connected to the computer through a communication interface; the sample cell is made of a transparent material. The device realizes online detection of the activity of the fermentation liquor yeast in the brewing process, has important significance for guiding the brewery to optimize the fermentation process, and can accurately and quickly detect the activity of the fermentation liquor yeast. However, the device directly uses the drainage sample from the fermentation tank, and the fermentation liquor which is directly drained is turbid liquid, and the Raman spectrum mainly detects transparent liquid, so that the device has low accuracy of online detection, and therefore, the device for online detection of the activity of the saccharomyces cerevisiae fermentation liquor yeast based on the Raman spectrum is provided.
Disclosure of Invention
The invention aims to solve the technical problem of providing an online detection device for the activity of saccharomyces cerevisiae fermentation liquor yeast based on Raman spectrum, which comprises a first water pump, wherein the first water pump is connected with a three-way valve through a water pipe, the three-way valve is connected to a detection box through a water pipe, the detection box is arranged on a spectrum detection device, a filter device for filtering wine liquid is arranged between the first water pump and the three-way valve, the three-way valve is also connected with a second water pump, the second water pump is connected to a clean water tank through a water pipe, the three-way valve is an electromagnetic valve, and the three-way valve intermittently opens the filter device and a channel between the second water pump and the detection box, so that the problem of low detection accuracy of the Raman spectrum.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a making wine zymotic fluid yeast vigor on-line measuring device based on raman spectrum, including first water pump, first water pump has the three-way valve through water piping connection, the three-way valve passes through the water piping connection to detect on the box, it establishes on spectrum detection device to detect the box, still be equipped with the filter equipment who is used for filtering wine liquid between first water pump and the three-way valve, the three-way valve still is connected with the second water pump, the second water pump passes through water piping connection to clean water tank on, the three-way valve is the solenoid valve, and the three-way valve intermittent type nature opens the passageway between filter equipment.
As a further scheme of the invention, a water pipe connected with a liquid inlet of the first water pump is connected to a liquid outlet of the fermentation tank through a manual three-way valve.
As a further scheme of the invention, a filter screen for filtering turbid substances in the wine liquid is arranged in the filter device, and the aperture of the filter screen is larger than the protein diameter of the wine liquid in the fermentation tank.
As a further scheme of the invention, distilled water is arranged in the cleaning water tank.
As a further scheme of the invention, the detection box is a square box body, the two ends of the detection box are both provided with interfaces for wine liquid circulation, the upper end interface of the detection box is connected to a liquid outlet of the three-way valve, and the lower end interface of the detection box is connected to the waste liquid pool.
As a further scheme of the invention, the lower end of the detection box is also provided with a second electromagnetic valve for closing an upper interface of the detection box.
As a further proposal of the invention, the filter device is also provided with a sediment outlet which is connected to the liquid inlet of the fermentation tank through a water pipe.
In conclusion, compared with the prior art, the invention has the following beneficial effects:
according to the invention, the wine liquid to be measured is filtered, and the cleaning process is added between the two adjacent measurement processes, so that the two adjacent measurement samples cannot influence each other, and meanwhile, the transparent liquid can improve the measurement precision.
Drawings
FIG. 1 is a schematic structural diagram of an online detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectroscopy.
FIG. 2 is a schematic structural diagram of a detection box in an online detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum.
Reference numerals: 1-fermentation tank, 2-first water pump, 3-filtering device, 4-three-way valve, 5-second water pump, 6-clean water tank, 7-detection box, 8-spectrum detection device, 81-laser, 82-Raman probe, 83-reflector, 84-spectrometer, 85-computer and 9-water pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Example 1
As shown in figure 1, the on-line detection device for the saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum comprises a first water pump 2, wherein the first water pump 2 is connected with a three-way valve 4 through a water pipe 9, the three-way valve 4 is connected to a detection box 7 through a water pipe 9, the detection box 7 is arranged on a spectrum detection device 8, a filter device 3 for filtering wine liquid is further arranged between the first water pump 2 and the three-way valve 4, the three-way valve 4 is further connected with a second water pump 5, the second water pump 5 is connected to a cleaning water tank 6 through a water pipe 9, the three-way valve 4 is an electromagnetic valve, and the three-way valve 4 intermittently opens a channel between the filter device 3 and the detection box 7;
a water pipe 9 connected with a liquid inlet of the first water pump 2 is connected to a liquid outlet of the fermentation tank 1 through a manual three-way valve, so that a worker can conveniently extract wine in the fermentation tank 1 and close a channel between the fermentation tank 1 and the first water pump 2;
a liquid outlet of the first water pump 2 is connected to a liquid inlet of the filtering device 3, a filter screen for filtering turbid substances in the wine liquid is arranged in the filtering device 3, and the aperture of the filter screen is larger than the diameter of protein of the wine liquid in the fermentation tank 1, so that the protein in the wine liquid can pass through the filtering device 3;
the three-way valve 4 is provided with two groups of liquid inlets and a group of liquid outlets, the liquid inlets on the three-way valve 4 are connected to the liquid outlets of the filtering device 3 and the second water pump 5, the liquid outlet of the three-way valve 4 is connected to the liquid inlet of the detection box 7, the three-way valve 4 intermittently opens a channel between the filtering device 3 and the detection box 7 and a channel between the second water pump 5 and the detection box 7, so that the detection box 7 is filled with wine liquid to be detected intermittently, and meanwhile, the detection box 7 is intermittently cleaned, so that test samples at two adjacent times are not influenced mutually, and distilled;
as shown in fig. 2, the detection box 7 is a square box body, both ends of the detection box 7 are provided with interfaces for wine to flow through, an upper end interface of the detection box 7 is connected to a liquid outlet of the three-way valve 4, a lower end interface of the detection box 7 is connected to the waste liquid pool, and the lower end of the detection box 7 is further provided with a second electromagnetic valve for closing a channel between the detection box 7 and the waste liquid pool;
the spectrum detection device 8 comprises a laser 81, a Raman probe 82 and a spectrometer 84, the Raman probe 82 is arranged on one surface of the detection box 7, a reflective mirror 83 is arranged on one surface of the detection box 7 far away from the Raman probe 82, the Raman probe 82 is also electrically connected with the spectrometer 84, the spectrometer 84 is electrically connected with a computer 85, the laser 81 emits laser with specific frequency, the laser emitted by the laser 81 irradiates the detection box 7 through reflection of the reflective mirror 83, a sample in the detection box 7 enables the laser to be scattered, the Raman probe 82 receives the scattered laser, the spectrometer 84 analyzes a Raman spectrum through the laser received by the Raman probe 82 and transmits the Raman spectrum to the computer 85, and the computer 85 analyzes the yeast activity of the sample to be detected through the frequency of the spectrum.
Example 2
As shown in figure 1, the on-line detection device for the saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum comprises a first water pump 2, wherein the first water pump 2 is connected with a three-way valve 4 through a water pipe 9, the three-way valve 4 is connected to a detection box 7 through a water pipe 9, the detection box 7 is arranged on a spectrum detection device 8, a filter device 3 for filtering wine liquid is further arranged between the first water pump 2 and the three-way valve 4, the three-way valve 4 is further connected with a second water pump 5, the second water pump 5 is connected to a cleaning water tank 6 through a water pipe 9, the three-way valve 4 is an electromagnetic valve, and the three-way valve 4 intermittently opens a channel between the filter device 3 and the detection box 7;
preferably, in this embodiment, the filtering device 3 is further provided with a precipitate outlet, and the precipitate outlet is connected to the liquid inlet of the fermentation tank 1 through a water pipe 9, so that the filtered liquid can return to the fermentation tank 1, and the waste of materials is reduced;
other structures in this embodiment are the same as those in embodiment 1.
In summary, the working principle of the invention is as follows:
filter equipment 3 can filter the large granule thing in the sample that awaits measuring for the sample that awaits measuring is transparent, thereby makes spectrum detection device 8 can accurately measure the spectral frequency of sample in detecting box 7, has improved measuring accuracy, and the inside that detects box 7 can be washd in the setting of second water pump 5, prevents that adjacent twice measuring sample from influencing each other, thereby influences measuring result.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a making wine zymotic fluid yeast vigor on-line measuring device based on raman spectrum, including first water pump (2), first water pump (2) are connected with three-way valve (4) through water pipe (9), three-way valve (4) are connected to on detecting box (7) through water pipe (9), it establishes on spectrum detection device (8) to detect box (7), a serial communication port, still be equipped with filter equipment (3) that are used for filtering the wine liquid between first water pump (2) and three-way valve (4), three-way valve (4) still are connected with second water pump (5), second water pump (5) are connected to on clean pond (6) through water pipe (9), three-way valve (4) are the solenoid valve, and three-way valve (4) intermittent type nature open filter equipment (3) and second water pump (5) and detect the passageway between box (7).
2. The on-line detection device for the saccharomyces cerevisiae fermentation broth yeast activity based on the raman spectrum according to claim 1, wherein a water pipe (9) connected with a liquid inlet of the first water pump (2) is connected to a liquid outlet of the fermentation tank (1) through a manual three-way valve.
3. The on-line detection device for the saccharomyces cerevisiae fermentation liquor yeast activity based on the raman spectrum as claimed in claim 1, wherein a filter screen for filtering turbid substances in the liquor is arranged in the filter device (3), and the pore diameter of the filter screen is larger than the protein diameter of the liquor in the fermentation tank (1).
4. The on-line detection device for saccharomyces cerevisiae fermentation liquor yeast activity based on raman spectrum according to claim 1, wherein distilled water is arranged in the cleaning water tank (6).
5. The on-line detection device for the saccharomyces cerevisiae fermentation broth yeast activity based on the raman spectrum according to claim 1, wherein the detection box (7) is a square box body, both ends of the detection box (7) are provided with interfaces for wine liquid circulation, the upper end interface of the detection box (7) is connected to a liquid outlet of the three-way valve (4), and the lower end interface of the detection box (7) is connected to a waste liquid pool.
6. The on-line detection device for the saccharomyces cerevisiae fermentation broth yeast activity based on the raman spectrum as claimed in claim 5, wherein the lower end of the detection box (7) is further provided with a second electromagnetic valve for closing an upper interface of the detection box (7).
7. The on-line detection device for the saccharomyces cerevisiae fermentation liquor yeast activity based on the raman spectrum according to any one of claims 1 to 6, wherein a precipitate outlet is further arranged on the filtering device (3), and the precipitate outlet is connected to a liquid inlet of the fermentation tank (1) through a water pipe (9).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010194513.6A CN111141724A (en) | 2020-03-19 | 2020-03-19 | On-line detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum |
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| CN202010194513.6A CN111141724A (en) | 2020-03-19 | 2020-03-19 | On-line detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112730376A (en) * | 2020-12-17 | 2021-04-30 | 山东省科学院生物研究所 | Device for detecting polysaccharide in fermentation liquor based on Raman spectrum, method and application thereof |
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| CN108753576A (en) * | 2018-06-15 | 2018-11-06 | 山东天力能源股份有限公司 | A kind of fermentation tank on-line period dilution system and its technique |
| CN110261510A (en) * | 2019-07-12 | 2019-09-20 | 上海舜宇恒平科学仪器有限公司 | A kind of on-line automatic sampling device detected for volatile organic matter in water |
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2020
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1306208A (en) * | 2001-02-26 | 2001-08-01 | 康达（成都）电子有限公司 | Automatic on-line monitor for yellow water acidity of pit of spirit factory |
| CN101477034A (en) * | 2009-01-13 | 2009-07-08 | 中国科学院南海海洋研究所 | On-line high-spectrum monitoring instrument for water trace element |
| US20140295532A1 (en) * | 2011-10-24 | 2014-10-02 | Bend Research, Inc. | Systems and methods for producing bioproducts |
| CN105548138A (en) * | 2016-01-13 | 2016-05-04 | 华南理工大学 | On-line wine making fermentation liquid yeast activity detection device and method based on Raman spectrum |
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| Publication number | Priority date | Publication date | Assignee | Title |
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