CN108753576A - A kind of fermentation tank on-line period dilution system and its technique - Google Patents
A kind of fermentation tank on-line period dilution system and its technique Download PDFInfo
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- 238000010790 dilution Methods 0.000 title claims abstract description 139
- 239000012895 dilution Substances 0.000 title claims abstract description 139
- 238000000855 fermentation Methods 0.000 title claims abstract description 29
- 230000004151 fermentation Effects 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000005070 sampling Methods 0.000 claims abstract description 94
- 238000001514 detection method Methods 0.000 claims abstract description 32
- 238000002156 mixing Methods 0.000 claims description 60
- 238000004140 cleaning Methods 0.000 claims description 46
- 230000002572 peristaltic effect Effects 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 28
- 239000000243 solution Substances 0.000 claims description 24
- 230000001954 sterilising effect Effects 0.000 claims description 19
- 238000005406 washing Methods 0.000 claims description 12
- 238000011144 upstream manufacturing Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 5
- 230000014759 maintenance of location Effects 0.000 claims description 5
- 230000000717 retained effect Effects 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 111
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 239000003085 diluting agent Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000012545 processing Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 1
- 238000000861 blow drying Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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Abstract
The present invention relates to a kind of fermentation tank on-line period dilution system and its techniques, it includes sampling system, dilution system and control system；Sampling system is connect with reactor, is automatically controlled by PLC, sampling is completed from reactor, and be filtered pretreatment to sample by filter.After having detected, steam and filtrated air can be passed through, entire sampling system is cleaned and sterilized, ensure that reactor is run with security and stability；Sample can be diluted in the detection range of detection sensor by dilution system, pass through the switching of valve so that dilution system can be run with high precision；The system and technique of the invention have many advantages, such as that safe, high degree of automation, precision are high.
Description
Technical field
The invention belongs to automatic on-line biochemistry detection technical fields, the specifically online sample obtained in real time in production process
Product, and suitably being diluted finally are detected a kind of fermentation tank on-line period dilution system and its work of Real-time Feedback result
Skill, the detection of online biochemical indicator suitable for biochemical produce.
Background technology
Procedure parameter is very important production target in biochemistry production, is related to the yield and quality etc. of final products.?
Line detection technique can obtain a large amount of required parameters in process of production, provide foundation for process real-time monitoring, ensure that
It is the stabilization of technical parameter in production process, controllable, it is also a large amount of artificial to be saved during production.It is rich with on-line parameter simultaneously
Richness also provides possibility for production intelligent control.
Existing online detection instrument has very much, and mainly for the physical-chemical parameters such as temperature, pH, dissolved oxygens, these equipment can
To be directly introduced in reactor, relevant parameter is directly obtained.But many biochemical parameters at present can't be straight in reaction process
Connect detection, mainly detection device does not adapt to the environment of reactor or material concentration in reactor not in the inspection of equipment
It surveys in range, therefore, in the prior art, also more biochemical parameter（Such as reduced sugar, total reducing sugar, organic acid, synthetic intermediate
Deng）It can not achieve real-time detection.
Invention content
To overcome the shortcomings of that above-mentioned existing online measuring technique, the present invention provide a kind of fermentation tank on-line period dilution system
And its technique.
The present invention adopts the following technical scheme that：A kind of fermentation tank on-line period dilution system, it is characterised in that：Including sampling
System, dilution system and control system；Sampling system one end is connect with reactor, and the other end is connect with dilution system；
The sampling system includes sampling pipe 18, for obtaining sample from reactor and conveying sample；
The dilution system includes sample fed unit to be diluted, sample amounts unit to be diluted, dilution fed unit, dilution
Liquid dosing unit, mixing pit 24；
Sample fed unit to be diluted is used to the sample in sampling system being fed to dilution system；
Sample amounts unit to be diluted includes triple valve A33, the triple valve B43 of two connections, and the ports triple valve A0 30, which correspond to, to be connected
The ports triple valve B0 40 are connect, the ports triple valve A1 31 connect sample fed unit to be diluted, the connection mixing of the ports triple valve B2 42
Pond 24,41 blowdown of the ports triple valve B1；
Dilution fed unit is used to dilution feeding dilution system；
Dilution dosing unit includes triple valve C53, the triple valve D63 of two connections, and the ports triple valve C0 50 are correspondingly connected with three
The ports port valve D0 60, the ports triple valve C1 51 connect dilution fed unit, and the ports triple valve D1 61 connect the ports triple valve A2
62 blowdown of the port 32, triple valve D2.
Further, in order to widen the detection range of equipment, greater proportion of product, the triple valve A and threeway are detected
The length of connecting pipe is variable between the ports A0-B0 between valve B；Connecting tube between the ports C0-D0 between triple valve C, triple valve D
The length in road is variable.
Further, it is filtered pretreatment in order to treat dilute sample, to sampling pipe pre-cleaning, improves accuracy,
Reduce error, the end of the sampling pipe is additionally provided with filter 13, and the filter bottom end is equipped with the blowdown with K6 valves 6
Pipe 2 15；Sampling pipe positioned at 13 upstream of filter is equipped with K1 valves 1 and K4 valves 4, is arranged between K1 valves and K4 valves
Blow-off pipe 1 with K3 valves 3,18 top of sampling pipe in 13 downstream of filter are connected with the exhaust pipe of K8 valves 8
Road.
Further, washing and sterilizing is carried out to sampling pipe in order to more convenient, is used convenient for next time, also avoided to reaction
Device impacts, and avoids influencing next accuracy of detection, compressed air pipe 1 and steam pipe are additionally provided in the sampling system
Road 21；One 19 beginning of the compressed air pipe is equipped with the sampling between K5 valves 5, its pipe end and K1 valves and K4 valves
Pipeline connects；The lateral that jet chimney 21 is equipped with K2 valves is connect with the compressed air pipe 1 of K5 valve downstreams, is steamed
Another lateral equipped with K7 valves 7 in steam pipe road connect with the sampling pipe in 13 downstream of filter and is communicated with discharge duct.
Further, for the ease of conveying sample to be diluted, the sample fed unit to be diluted includes sample to be diluted
Supply conduit and the first peristaltic pump 22 being located on the pipeline, the sample supply conduit to be diluted of the first peristaltic pump upstream are additionally provided with
K9 valves 9.
Further, it for the ease of cleaning dilution system, avoids influencing next accuracy of detection, 22 upstream of the first peristaltic pump is also
Equipped with Cleaning pipes one and Cleaning pipes two.
Further, for the ease of conveying dilution, dilution fed unit includes dilution supply conduit and is located at this
The second peristaltic pump 23 on pipeline.
Further, for the folding of control valve, the switching of triple valve, the rotation of peristaltic pump, sample and dilution
Into materials quantitative, the control system is PLC control system.
A kind of on-line automatic sampling dilution technique, including：
（1）Sampling, sampling system are sampled from reactor, and sample enters in sampling pipe 18；
（2）Dilution is quantitative, and sample in sampling system is fed dilution system by sample fed unit to be diluted, and sample passes through triple valve
A and triple valve B is input to triple valve B1 port blowdowns, closes sample fed unit to be diluted, switching three-way valve A and triple valve B
Port, while retaining the sample in triple valve A0-B0 pipelines；It is passed through compressed air, it will be in pipeline between retention triple valve A0-B0
Sample input mixing pit completes being quantitatively adding for sample；Dilution fed unit is defeated from triple valve C and triple valve D by dilution
Enter to triple valve D2 port blowdowns, closes diluent liquid fed unit, switching three-way valve C and the ports triple valve D, while retaining three
Dilution between the ports port valve C0-D0 in pipeline, is passed through compressed air, shifts the dilution in C0-D0 pipelines onto mixing pit, complete
At being quantitatively adding for dilution；
（3）Hybrid detection, sample and dilution to be diluted refer to after the mixing of mixing pit 24 into biochemistry is detected in detection sensor
Mark, and then obtain final result.
Further, enter sampling system, step for the ease of flexibly controlling liquid in reactor（1）It is middle to utilize reactor
Liquid dead weight or sampling pump sampling enter sampling system.
Further, a kind of technique of fermentation tank on-line period dilution system, including：
（1）Sampling, sampling system are sampled from reactor, and sample enters pre-cleaning in sampling pipe and filters, control system control
K1 valves, K3 valves, K4 valves, K6 valves, K8 valves are opened, and retaining samples are discharged；Sample is filtered by fore filter, is obtained
To pretreated sample, K4, K8 valve are finally closed, complete to sample and processing is filtered to sample.
（2）Dilution is quantitative, and pretreated sample is fed dilution system by sample fed unit to be diluted, and sample passes through three
Port valve A and triple valve B is input to triple valve B1 port blowdowns, closes sample fed unit to be diluted, switching three-way valve A and threeway
The ports valve B, while retaining the sample between the ports triple valve A0-B0 in pipeline；Logical compressed air, will be between retention triple valve A0-B0
Sample input mixing pit in pipeline, completes being quantitatively adding for sample；Dilution fed unit is by dilution from triple valve C and three
Port valve D is input to triple valve D2 port blowdowns, closes diluent liquid fed unit, switching three-way valve C and the ports triple valve D, together
When retention triple valve C0-D0 ports between dilution in pipeline, compressed air is passed through, by the dilution in triple valve C0-D0 pipelines
Liquid shifts mixing pit onto, completes being quantitatively adding for dilution；
（3）Hybrid detection, sample and dilution to be diluted refer to after mixing pit mixing into biochemistry is detected in detection sensor
Mark, and then obtain final result.
（4）Washing and sterilizing empties remaining sample by blow-off pipe, then opens K2 valves, K4 valves, K7 valves, leads to steam
It is rinsed sterilizing, after sterilizing, K2 valves, K7 valves is closed, opens K5 valves, aseptic compressed air is passed through, to entire
Pipeline is dried up and is cooled down, and is finally closed K3, K5, K6, K8 valve, is completed the washing and sterilizing of automatic sampling system；
Cleaning solution a is pumped by triple valve A and triple valve B in blowdown and mixing pit by the first peristaltic pump, empties mixing pit；Again
Cleaning solution b is pumped by triple valve A and triple valve B in mixing pit, empties mixing pit again, finally clear with cleaning solution a
Entire dilution system is washed, mixing pit is emptied, completes the cleaning of dilution system.
Technical solution provided by the invention has the following advantages that：
1. the on-line checking of biochemical parameter can be realized, do not influenced by reactor service condition, has also widened the inspection of detection device
Survey range；
2. system runs without influence reactor, sampling system can be effectively in isolated environment to the influence factor of reactor, such as
Microorganism, air etc.；
3. dilution system precision is high, dilution and detection error range≤5%；
4. equipment automatization is run, operating cost is low, and compared with prior art, system data acquisition density is high, saves a large amount of people
Work.
Description of the drawings
Fig. 1 is sampling system flow chart provided by the invention；
Fig. 2 is dilution system flow chart provided by the invention.
Wherein, 1.K1 valves, 2.K2 valves, 3.K3 valves, 4.K4 valves, 5.K5 valves, 6.K6 valves, 7.K7 valves,
8.K8 valves, 9.K9 valves, 10.K10 valves, 11.K11 valves, 12.K12 valves, 13. filters, 14. blow-off pipes one, 15.
Blow-off pipe two, 16. blow-off pipe, 3 17. blow-off pipe four, 18. sampling pipes, 19. compressed air pipes one, 20. compressed air pipes
Two, 21. jet chimneys, 22. first peristaltic pumps, 23. second peristaltic pumps, 24. mixing pits, 30. ports triple valve A0,31. threeways
The ports valve A1,32. ports triple valve A2,33. triple valve A, 40. ports triple valve B0,41. ports triple valve B1,42. triple valves
The ports B2,43. triple valve B, 50. ports triple valve C0,51. ports triple valve C1,52. ports triple valve C2,53. triple valve C,
60. the ports triple valve D0,61. ports triple valve D1,62. ports triple valve D2,63. triple valve D, 71. Cleaning pipes one, 72.
Cleaning pipes two.
Specific implementation mode
1, attached drawing 2 and embodiment are described further technical solution of the present invention below in conjunction with the accompanying drawings.
A kind of fermentation tank on-line period dilution system, including sampling system, dilution system and control system；The control system
System is PLC control system.
As shown in Figure 1, for the sampling system flow chart in fermentation tank on-line period dilution system provided by the invention, sampling
System one end is connect with reactor, and the other end is connect with dilution system；Reaction solution in reactor by tank bottom pressure pipeline from
Stream or sampling pump, sample is transported to from reactor in sampling system；Sampling system includes that sampling pipe 18 and setting are sampling
Valve on pipeline；
The end of the sampling pipe is equipped with filter 13 and in communication, connection sample to be diluted in the filter screen of filter 13
Fed unit, blow-off pipe two 15 of the filter bottom end connection with K6 valves 6.
Sampling pipe 18 positioned at 13 upstream of filter is equipped with K1 valves 1 and K4 valves 4, is set between K1 valves and K4 valves
The blow-off pipe 1 with valve K3 is set, the discharge duct of valve K8 is connected with above the sampling pipe in 13 downstream of filter.
The filter 13 is preferably fore filter.
The sampling system is additionally provided with compressed air pipe 1 and jet chimney 21；The compressed air pipe 1 begins
End connects sampling pipe 18 equipped with K5 valves 5, its pipe end, specifically, one 19 end of compressed air pipe and K1 valves 1 and
Sampling pipe between K4 valves 4 is connected and is communicated with blow-off pipe 1；Jet chimney 21 be equipped with K2 valves 2 lateral with
The compressed air pipe 1 in 5 downstream of K5 valves connects, jet chimney 21 another lateral and probe tube equipped with K7 valves 7
The end connection in road is specifically connected on the pipeline in fore filter downstream and is connected with the discharge duct equipped with K8 valves 8,
K8 valves are located on the outside of jet chimney, do not influence jet chimney circulation, and the folding for controlling discharge duct makes in sampling pipe
Sample operation it is more smooth, do not generate gas blocking.
Control system controls K1 valves, K3 valves, K4 valves, K6 valves, K8 valves and opens, and passes through K3 valves, K6 valves
The sample being detained in discharge sampling pipe, after a period of time, K3 valves, K6 valves are closed, and sample is filtered by fore filter, mistake
Sample after filter is kept in the filter, and pretreated sample is obtained, and finally closes K4, K8 valve, completes sampling and to sample
It is filtered processing.
Automatic on-line sampling system operation mechanism：By PLC automatic control system, control valve being opened and closing, using anti-
It answers liquid dead weight in device or is equipped with sampling pump sampling, liquid is filtered processing by fore filter, and filtered liquid exists
Detection to be diluted in filter.After the completion of to be detected, discharge all liq in sampling system, and logical steam to whole system into
Row cleaning and sterilizing, finally lead to aseptic compressed air, blow-drying system pipeline, system is on reactor without influence, effective isolated environment
In to the influence factor of reactor.
As shown in Fig. 2, dilution system includes sample fed unit to be diluted, sample amounts unit to be diluted, diluent liquid
Fed unit, diluent liquid dosing unit, also mixing pit.
Sample fed unit to be diluted is used for the sample delivery in sampling system to dilution system, including sample to be diluted
The sample to be diluted of supply conduit and the first peristaltic pump 22 being located on the pipeline, the connection of 22 inlet upstream of the first peristaltic pump is fed
Pipeline is additionally provided with K9 valves 9, and K9 valves are used to control the folding of pipeline at this, control the flowing of sample.
First peristaltic pump, 22 inlet upstream is also connected with the Cleaning pipes 1 equipped with K10 valves 10 and is equipped with K11 valves 11
Cleaning pipes 2 72, Cleaning pipes one 71 logical cleaning solution a, 2 72 cleaning solution b of Cleaning pipes.
Cleaning solution a is generally distilled water, and cleaning solution b is generally organic washing solvent, and different according to detection sample select
Different cleaning solutions.
Sample amounts unit to be diluted includes triple valve A33, the triple valve B43 of two connections, and there are three ends for triple valve tool
Mouthful, the ports triple valve A0 30 are correspondingly connected with the ports triple valve B0 40, and the ports triple valve A1 31 connect sample fed unit to be diluted
The first peristaltic pump 22 outlet, the ports triple valve B2 42 connect mixing pit 24, the ports triple valve B1 41 pass through blow-off pipe 3 16
Blowdown；
Dilution fed unit is used to dilution feeding dilution system, including dilution supply conduit and is located on the pipeline
Second peristaltic pump 23.
Dilution dosing unit includes triple valve C53, the triple valve D63 of two connections, and the ports triple valve C0 50, which correspond to, to be connected
The ports triple valve D0 60 are connect, the ports triple valve C1 51 connect the second peristaltic pump 23 of dilution fed unit, the ports triple valve C2
52 are connected with the compressed air pipe 2 20 of K12 valves 12, and the ports triple valve D1 61 connect the ports triple valve A2 32, triple valve
The ports D2 62 connect 17 blowdown of blow-off pipe.
It is worth noting that, the A0-B0 connecting pipe length between the ports triple valve A0 30 and the ports triple valve B0 40 can
Change；C0-D0 connecting pipes length between the ports triple valve C0 50 and the ports triple valve D0 60 can also change, and can pass through
The amount that sample and dilution is added in the adjustment of the distance between duct length, two triple valves is adjusted, detection model has greatly been widened
It encloses, it is high that data acquire density, has saved a large amount of manpowers, the ratio of the sample detected as needed, design sample and dilution
Amount, sets the length of corresponding pipeline between triple valve.
The sample after pretreatment filtration is drawn to automatic dilution system by the first peristaltic pump 22, sample passes through triple valve
A and B, until sample enters blow-off pipe three full of this segment pipe, closes the first peristaltic pump 22 and K9 by A1-A0-B0-B1 mouthfuls
The ports triple valve A1, B1 of triple valve A and B are switched to the ports triple valve A2, B2, while retaining triple valve A0-B0 by valve 9
Sample in pipeline opens K12 valves 12, is passed through aseptic compressed air, and by A2-A0-B0-B2, retention sample input is arrived
In mixing pit 24, sample being completed at this time and is quantitatively adding, volume of the addition between the A0-B0 of triple valve port in pipeline can be with
The amount that sample is added is adjusted by adjusting duct length.The connection mouth of triple valve C and D are switched to triple valve by same reason
Dilution is entered blow-off pipe by the ports C1 and D2, the second peristaltic pump 23 from the connection port C1-C0-D0-D2 of triple valve C and D
Four, the second peristaltic pump 23 is closed, the port C1, D2 of triple valve C and D are switched to the port C2, D1, while retaining triple valve C0-
Dilution in D0 pipelines opens K12 valves 12, compressed air is passed through, by triple valve C, D, A, B, by C2-C0-D0-
It D1-A2-B2 mouthfuls, shifts the dilution in triple valve C0-D0 pipelines onto mixing pit 24, is quantitatively adding dilution, likewise, can be with
The amount that diluent liquid is added is adjusted by adjusting duct length.After the mixing of mixing pit 24, so that it may dilute by a certain percentage to obtain
The sample released；After sample dilutes well, into the biochemical indicator detected in detection sensor in sample, and logarithm is converted
The final result of feedback parameter afterwards.
After parameter detecting is complete, need to clean sampling system and dilution system.Sampling system first open K3 valves and
K6 valves, the sample in emptying system, then K2 valves 2, K4 valves 4, K7 valves 7 are opened, it is passed through steam, residual sample is carried out
It rinses, and sterilization treatment is carried out to entire sampling system, prevent pollution reactor and influence next group detection as a result, sterilizing knot
Shu Hou closes K2, K7 valve, opens K5 valves 5, is passed through aseptic compressed air, entire pipeline is dried up and cooled down, finally
K3, K5, K6, K8 valve are closed, the washing and sterilizing of automatic sampling system is completed.
Dilution system also needs to clean, and by valve transfer, opens the first peristaltic pump 22 and K10 valves 10, will clean
Liquid a is pumped by triple valve A and triple valve B in blowdown and mixing pit 24, while emptying mixing pit, closes K10 valves, is opened
Cleaning solution b is pumped by triple valve A and triple valve B in mixing pit, empties mixing pit again by K11 valves, finally with cleaning
Liquid a cleans entire dilution system, empties mixing pit, completes the cleaning of dilution system.The cleaning of triple valve C and D can also use
Two peristaltic pumps 23 are pumped into water, and either cleaning solution is cleaned or other modes.
Dilution system mechanism：Automatic dilution is exactly to carry out a certain proportion of dilution to sample with water or other dilutions, is closed
Key point is exactly the accurate quantitative analysis to liquid, and the present invention is by using 2 groups（4）Three-way magnetic valve quantifies sample and dilution respectively
Water, to reach a certain proportion of purpose of dilution.The folding and switching of solenoid valve and peristaltic pump are controlled by PLC, control sample
Charging with water is with quantitatively, and the liquid after being quantified by compressed air blows into mixing pit, and the amount of sample and water then passes through 1 group
Pipeline length between triple valve controls.After the completion of dilution, pipeline is cleaned by cleaning solution, avoids influencing next time
Testing result is diluted, data precision improves.
The technique of the fermentation tank on-line period dilution system is：（1）Sampling, liquid is by conducting oneself with dignity or with being on the waiting list in reactor
Sample pumping sample, sample enter in sampling pipe 18；
（2）Dilution is quantitative, and sample to be diluted is fed dilution system by sample fed unit to be diluted, and sample passes through triple valve A33
With triple valve B43, until sample enters blow-off pipe 3 16 full of this segment pipe, sample to be diluted is closed by A1-A0-B0-B1 mouthfuls
Product fed unit, switching three-way valve A and triple valve B are retained to the ports triple valve A2, B2 in triple valve A0-B0 pipelines
Sample opens K12 valves 12, is passed through aseptic compressed air, will be retained by A2-A0-B0-B2 by triple valve C, D, A, B
In sample input to mixing pit 24 in A0-B0 pipelines, being quantitatively adding for sample is completed；By triple valve C53's and triple valve D63
Connect mouth and be switched to the ports triple valve C1 51 and the ports triple valve D2 62, dilution fed unit by dilution from triple valve C and
The connection mouth C1-C0-D0-D2 of triple valve D enters blow-off pipe 4 17, dilution fed unit device is then shut off, by threeway
C1, D2 mouth of valve C and D are switched to C2, D1 mouthfuls, while retaining the diluent liquid in triple valve C0-D0 pipelines, open K12 valves
12, it is passed through compressed air, by triple valve C, D, A, B, by C2-C0-D0-D1-A2-B2 mouthfuls, by the dilution in C0-D0 pipelines
Liquid shifts mixing pit 24 onto, completes being quantitatively adding for dilution；
（3）Hybrid detection, sample to be diluted and diluent liquid are after the mixing of mixing pit 24, the sample that has been diluted by a certain percentage
Product；Into in detection sensor detect sample in biochemical indicator, and logarithm converted after feedback parameter final result.
The technique（1）In further include pre-cleaning filtration step, sampling start when, pass through PLC robot control system(RCS)s, control K1 valves
Door, K3 valves, K4 valves, K6 valves, K8 valves are opened, and liquid reactor is pressed into sampling system, K3 valves, K6 are passed through
Retaining samples in sampling pipe are discharged in valve, after a period of time, close K3 valves, K6 valves, for sample by pressure, process is preposition
Filter filters, and obtains pretreated sample, finally closes K4 valves, K8 valves, completes to sample and be filtered sample
Processing.
Technique（3）After the completion of detection, which further includes washing and sterilizing system, is first turned on K3, K6 valve, empties pipeline
The sample of middle remnants, then K2, K4, K7 valve are opened, logical steam is rinsed residual sample, and is carried out to entire sampling system
Sterilization treatment after sterilizing, closes K2, K7 valve, opens K5 valves, be passed through aseptic compressed air, carried out to entire pipeline
Drying and cooling, finally close K3, K5, K6, K8 valve, complete the washing and sterilizing of automatic sampling system；
The first peristaltic pump 22 and K10 valves are opened, cleaning solution a, generally distilled water are pumped by triple valve A and triple valve B
Into blowdown and mixing pit 24, while by the emptying rate of liquid in mixing pit, K10 valves 10 are then shut off, open K11 valves 11,
By cleaning solution b, generally organic washing solvent, it is pumped into mixing pit by triple valve A and B, empties mixing pit again, finally
Whole system is being cleaned with cleaning solution a, mixing pit is being emptied, has completed the cleaning of dilution system.
By taking reduced sugar detection in microorganism fermentation tank fermentation process as an example：
Automatic sampling system equipment in Fig. 1 is connected to fermentation tank middle and lower part sample tap when sampling starts and passes through PLC automatic controls system
System, control K1 valves, K3 valves, K4 valves, K6 valves, K8 valves are opened, and fermentation broth sample, will by fermentation tank autogenou pressure
Liquid is pressed into sampling system, and retaining samples in sampling pipe are discharged by K3 valves, K6 valves, after a period of time, close
K3 valves, K6 valves, liquid filter by fore filter 13 by pressure, obtain pretreated fermentation broth sample（Filtering
Sample is stored in the U-shaped filter screen of filter）, K4 valves, K8 valves are finally closed, completes to sample and be filtered sample
Processing.
The zymotic fluid pre-processed is diluted, the first peristaltic pump 22 is opened and K9 valves 9, liquid passes through triple valve A
And B, until sample enters sewage draining exit, closes the first peristaltic pump 22 and K9 valves 9, by triple valve by the ports A1-A0-B0-B1
The port A1, B1 of A and B is switched to the port A2, B2, while retaining the sample in triple valve A0-B0 pipelines, opens K12 valves, leads to
Enter compressed air, shifts the sample in A0-B0 pipelines onto by C2-C0-D0-D1-A2-B2 mouthfuls by triple valve C, D, A, B
Mixing pit 24 is completed sample amounts and is added；Then the connection mouth of triple valve C and D are switched to the ports C1 and D2, it is compacted opens second
Dynamic pump 23, dilution water enters blowdown from the connection mouth C1-C0-D0-D2 of triple valve C and D, is then shut off the second peristaltic pump 23,
C1, D2 mouth of triple valve C and D are switched to C2, D1 mouthfuls, while retaining the dilution water in triple valve C0-D0 pipelines, opens K12
Valve 12, is passed through compressed air, will be in pipeline between C0-D0 by C2-C0-D0-D1-A2-B2 mouthfuls by triple valve C, D, A, B
Dilution water shift mixing pit 24 onto, complete being quantitatively adding for dilution water；After sample and dilution water mix in mixing pit, obtain
Diluent concentration can be within the scope of the Concentration Testing of reduced sugar sensor, can be by adjusting between A0-B0 between pipeline and C0-D0
The length of pipeline, to adjust diluted multiple, so as to complete the on-line checking of sample.After having detected, the result of feedback passes through
Parameter conversion is crossed, the concentration of reduced sugar in zymotic fluid is obtained.
The system for completing detection is cleaned, and is prepared for detection next time.Since ferment tank needs to prevent
The only infection of miscellaneous bacteria, therefore with tank connected sampling system of fermenting, need the cleaning for preventing dye miscellaneous bacteria that setting is stringent.
It is first turned on K3, K6 valve, sample remaining in pipeline is emptied, then open K2, K4, K7 valve, steam is passed through, to residual sample
It is rinsed, and sterilization treatment is carried out to entire sampling system, after sterilizing, close K2, K7 valve, open K5 valves, lead to
Enter aseptic compressed air, entire pipeline is dried up and cooled down, finally close K3, K5, K6, K8 valve, completes automatic sampling
The washing and sterilizing of system.
Remaining sample can influence the accuracy of the testing result of next group sample in automatic dilution system, but not with hair
Fermentation tank is directly connected to, therefore cleaning process does not have sterile requirement, the first peristaltic pump 22 and K10 valves 10 is opened, by cleaning solution a
（Generally distilled water）It is pumped into blowdown and mixing pit by triple valve A and B, while by the emptying rate of liquid in mixing pit 24,
K10 valves 10 are then shut off, K11 valves 11 are opened, by cleaning solution b（Generally organic washing solvent）It is pumped by triple valve A and B
Enter into mixing pit, empty mixing pit again, finally cleaning whole system with cleaning solution a, emptied mixing pit, completes dilution
The cleaning of system.
Particular embodiments described above has carried out further in detail the purpose of the present invention, technical solution and advantageous effect
It describes in detail bright, it should be understood that the above is only a specific embodiment of the present invention, is not intended to restrict the invention, it is all
Within the spirit and principles in the present invention, any modification, equivalent substitution, improvement and etc. done should be included in the guarantor of the present invention
Within the scope of shield.
Claims (12)
1. a kind of fermentation tank on-line period dilution system, it is characterised in that：Including sampling system, dilution system and control system；
Sampling system one end is connect with reactor, and the other end is connect with dilution system；
The sampling system includes sampling pipe（18）, for obtaining sample from reactor and conveying sample；
The dilution system includes sample fed unit to be diluted, sample amounts unit to be diluted, dilution fed unit, dilution
Liquid dosing unit, mixing pit（24）；
Sample fed unit to be diluted is used to the sample in sampling system being fed to dilution system；
Sample amounts unit to be diluted includes the triple valve A of two connections（33）, triple valve B（43）, the ports triple valve A0（30）
Connect the ports triple valve B0（40）, the ports triple valve A1（31）Connect sample fed unit to be diluted, the ports triple valve B2（42）
Connect mixing pit, the ports triple valve B1（41）Blowdown；
Dilution fed unit is used to dilution feeding dilution system；
Dilution dosing unit includes the triple valve C of two connections（53）, triple valve D（63）, the ports triple valve C0（50）Connection
The ports triple valve D0（60）, the ports triple valve C1（51）Connect dilution fed unit, the ports triple valve D1（61）Connect threeway
The ports valve A2（32）, the ports triple valve D2（62）Blowdown.
2. fermentation tank on-line period dilution system according to claim 1, it is characterised in that：The triple valve A（33）With
Triple valve B（43）Between the ports A0-B0 between connecting pipe length it is variable；Triple valve C（53）, triple valve D（63）Between
The length of connecting pipe is variable between the ports C0-D0.
3. fermentation tank on-line period dilution system according to claim 1 or 2, it is characterised in that：The sampling pipe
（18）End be additionally provided with filter（13）, the filter（13）Bottom end, which is equipped with, carries K6 valves（6）Blow-off pipe two（15）；
Sampling pipe positioned at filter upstream（18）Equipped with K1 valves（1）With K4 valves（4）, band is set between K1 valves and K4 valves
There are K3 valves（3）Blow-off pipe one（14）, the sampling pipe top of filter downstream is connected with K8 valves（8）Exhaust pipe
Road.
4. fermentation tank on-line period dilution system according to claim 3, it is characterised in that：It is also set in the sampling system
There is compressed air pipe one（19）And jet chimney（21）；One beginning of the compressed air pipe is equipped with K5 valves（5）, its pipeline
End and K1 valves（1）With K4 valves（4）Between sampling pipe（18）Connection；Jet chimney（21）Equipped with K2 valves（2）'s
Lateral and K5 valves（5）The compressed air pipe one in downstream（19）Connection, jet chimney（21）It is another to be equipped with K7 valves（7）
Lateral and filter（13）The sampling pipe in downstream is connected and is communicated with discharge duct.
5. fermentation tank on-line period dilution system according to claim 1 or 2, it is characterised in that：The sample to be diluted
Fed unit includes sample supply conduit to be diluted and the first peristaltic pump for being located on the pipeline（22）, the first peristaltic pump upstream
Sample supply conduit to be diluted is additionally provided with K9 valves（9）.
6. fermentation tank on-line period dilution system according to claim 4, it is characterised in that：The sample to be diluted is fed
Unit includes sample supply conduit to be diluted and the first peristaltic pump for being located on the pipeline（22）, waiting for for the first peristaltic pump upstream be dilute
It releases sample supply conduit and is additionally provided with K9 valves（9）.
7. fermentation tank on-line period dilution system according to claim 6, it is characterised in that：First peristaltic pump（22）Upstream
It is additionally provided with Cleaning pipes one（71）With Cleaning pipes two（72）.
8. fermentation tank on-line period dilution system according to claim 1 or 2, it is characterised in that：Dilution fed unit
Including dilution supply conduit and the second peristaltic pump being located on the pipeline（23）.
9. fermentation tank on-line period dilution system according to claim 1, it is characterised in that：The control system is PLC
Control system.
10. a kind of technique including the fermentation tank on-line period dilution system described in claim 1-9 any claims, packet
It includes：
（1）Sampling, sampling system are sampled from reactor, and sample enters sampling pipe（18）In；
（2）Dilution is quantitative, and sample in sampling system is fed dilution system by sample fed unit to be diluted, and sample passes through triple valve
A（33）With triple valve B（43）Be input to triple valve B1 port blowdowns, close sample fed unit to be diluted, switching three-way valve A and
The ports triple valve B, while retaining the sample in triple valve A0-B0 pipelines；It is passed through compressed air, it will be between retention triple valve A0-B0
Sample input mixing pit in pipeline（24）, complete being quantitatively adding for sample；Dilution fed unit is by dilution from triple valve C
（53）With triple valve D（63）Triple valve D2 port blowdowns are input to, dilution fed unit, switching three-way valve C and threeway are closed
The ports valve D, while the dilution in triple valve C0-D0 pipelines is retained, it is passed through compressed air, it will be in pipeline between triple valve C0-D0
Dilution shift mixing pit onto, complete being quantitatively adding for dilution；
（3）Hybrid detection, sample and dilution to be diluted are in mixing pit（24）It is biochemical into being detected in detection sensor after mixing
Index, and then obtain final result.
11. the technique of fermentation tank on-line period dilution system according to claim 10, it is characterised in that：Step（1）In
Enter sampling system using the liquid dead weight or sampling pump sampling of reactor.
12. a kind of technique including the fermentation tank on-line period dilution system described in claim 7, including：
（1）Sampling, sampling system are sampled from reactor, are carried out the pre-cleaning filtering of sample, are controlled K1 valves（1）, K3 valves
（3）, K4 valves（4）, K6 valves（6）, K8 valves（8）It opens, retaining samples is discharged；Sample to be diluted is through filter（13）It crosses
Filter, obtains pretreated sample, finally closes K4 valves（4）, K8 valves（8）, complete to sample and place be filtered to sample
Reason.
（2）Dilution is quantitative, and the sample after filtration treatment is fed dilution system by sample fed unit to be diluted, and sample passes through threeway
Valve A（33）With triple valve B（43）It is input to the ports triple valve B1（41）Sample fed unit to be diluted, switching three are closed in blowdown
The ports port valve A and B, while retaining the sample between triple valve A0-B0 in pipeline；It is passed through compressed air, triple valve A0-B0 will be retained
Between sample input mixing pit in pipeline, complete being quantitatively adding for sample；Dilution fed unit is by dilution from triple valve C
（53）With triple valve D（63）It is input to the ports triple valve D2（62）Dilution fed unit, switching three-way valve C and D are closed in blowdown
Port retains the dilution in triple valve C0-D0 pipelines, compressed air is passed through, by the dilution in pipeline between triple valve C0-D0
It shifts mixing pit onto, completes being quantitatively adding for dilution；
（3）Hybrid detection, sample and dilution to be diluted are in mixing pit（24）It is biochemical into being detected in detection sensor after mixing
Index, and then obtain final result.
（4）Washing and sterilizing empties remaining sample by blow-off pipe, opens K2 valves, K4 valves, K7 valves, and logical steam flush is gone out
Bacterium, sterilizing terminate, and close K2 valves, K7 valves, open K5 valves, lead to aseptic compressed air, to entire pipeline carry out drying and
Cooling finally closes K3, K5, K6, K8 valve, completes the washing and sterilizing of automatic sampling system；
First peristaltic pump（22）Cleaning solution a is pumped into blowdown and mixing pit by triple valve A and triple valve B（24）In, emptying
Mixing pit；Cleaning solution b is pumped by triple valve A and triple valve B in mixing pit again, empties mixing pit again, finally with
Cleaning solution a cleans entire dilution system, empties mixing pit, completes the cleaning of dilution system.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110672375A (en) * | 2019-10-15 | 2020-01-10 | 上海宝钢工业技术服务有限公司 | Online sampling and cleaning system for electrotinning solution |
| CN111141724A (en) * | 2020-03-19 | 2020-05-12 | 茅台学院 | On-line detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum |
| CN113375980A (en) * | 2021-05-08 | 2021-09-10 | 上海环境卫生工程设计院有限公司 | Online sampling system and sampling method for wet garbage anaerobic digestion material |
| CN115184441A (en) * | 2022-07-26 | 2022-10-14 | 山东艾科森特生物科技有限公司 | Online detection device and method for multi-channel enzyme electrode in fermentation production process |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1081254A (en) * | 1991-11-22 | 1994-01-26 | 科特电子公司 | Fluid metering, mixing, transmission valve member and use the analytic system of this assembly |
| JPH06307994A (en) * | 1993-03-17 | 1994-11-04 | Sumitomo Metal Mining Co Ltd | Dilution equipment and automatic sampling system for high concentration liquid |
| JPH09304248A (en) * | 1996-05-10 | 1997-11-28 | Dkk Corp | Weighing apparatus and dilution apparatus |
| CN1402009A (en) * | 2002-09-27 | 2003-03-12 | 江南大学 | Sampling diluting device for bio-reaction process in-line testing system |
| CN2634481Y (en) * | 2003-05-08 | 2004-08-18 | 山东省科学院中日友好生物技术研究中心 | On-line automatic detector for reduced sugar |
| CN103698157A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Special sampling device for fermentation tank |
| CN103712902A (en) * | 2013-12-31 | 2014-04-09 | 鞍山钢铁集团公司 | Hematology analyzer adopting single negative pressure source and pipeline flow regulator |
| CN205049400U (en) * | 2015-10-14 | 2016-02-24 | 郑州富铭环保科技有限公司 | A diluting device that is used for quality of water on line analyzer survey high concentration water sample |
| CN107449882A (en) * | 2017-07-24 | 2017-12-08 | 东华工程科技股份有限公司 | A kind of high-concentration sewage COD on-line monitoring systems |
| CN206736244U (en) * | 2017-05-12 | 2017-12-12 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of lactobacillus-fermented sampler |
| CN107607596A (en) * | 2017-08-08 | 2018-01-19 | 福建农林大学 | A kind of online test method and device of the sulfate pulping process paper pulp kappa number based on flow analysis |
| CN208632519U (en) * | 2018-06-15 | 2019-03-22 | 山东天力能源股份有限公司 | A kind of fermentor on-line period dilution system |
-
2018
- 2018-06-15 CN CN201810617368.0A patent/CN108753576A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1081254A (en) * | 1991-11-22 | 1994-01-26 | 科特电子公司 | Fluid metering, mixing, transmission valve member and use the analytic system of this assembly |
| JPH06307994A (en) * | 1993-03-17 | 1994-11-04 | Sumitomo Metal Mining Co Ltd | Dilution equipment and automatic sampling system for high concentration liquid |
| JPH09304248A (en) * | 1996-05-10 | 1997-11-28 | Dkk Corp | Weighing apparatus and dilution apparatus |
| CN1402009A (en) * | 2002-09-27 | 2003-03-12 | 江南大学 | Sampling diluting device for bio-reaction process in-line testing system |
| CN2634481Y (en) * | 2003-05-08 | 2004-08-18 | 山东省科学院中日友好生物技术研究中心 | On-line automatic detector for reduced sugar |
| CN103698157A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Special sampling device for fermentation tank |
| CN103712902A (en) * | 2013-12-31 | 2014-04-09 | 鞍山钢铁集团公司 | Hematology analyzer adopting single negative pressure source and pipeline flow regulator |
| CN205049400U (en) * | 2015-10-14 | 2016-02-24 | 郑州富铭环保科技有限公司 | A diluting device that is used for quality of water on line analyzer survey high concentration water sample |
| CN206736244U (en) * | 2017-05-12 | 2017-12-12 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of lactobacillus-fermented sampler |
| CN107449882A (en) * | 2017-07-24 | 2017-12-08 | 东华工程科技股份有限公司 | A kind of high-concentration sewage COD on-line monitoring systems |
| CN107607596A (en) * | 2017-08-08 | 2018-01-19 | 福建农林大学 | A kind of online test method and device of the sulfate pulping process paper pulp kappa number based on flow analysis |
| CN208632519U (en) * | 2018-06-15 | 2019-03-22 | 山东天力能源股份有限公司 | A kind of fermentor on-line period dilution system |
Non-Patent Citations (2)
| Title |
|---|
| 杨海麟等: "一种新型在线级联稀释系统的研究", 《现代科学仪器》, vol. 5, pages 19 - 22 * |
| 杨海麟等: "生物反应过程在线检测系统的研制", 《无锡轻工大学学报》, vol. 22, no. 1, pages 33 - 37 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110672375A (en) * | 2019-10-15 | 2020-01-10 | 上海宝钢工业技术服务有限公司 | Online sampling and cleaning system for electrotinning solution |
| CN111141724A (en) * | 2020-03-19 | 2020-05-12 | 茅台学院 | On-line detection device for saccharomyces cerevisiae fermentation broth yeast activity based on Raman spectrum |
| CN113375980A (en) * | 2021-05-08 | 2021-09-10 | 上海环境卫生工程设计院有限公司 | Online sampling system and sampling method for wet garbage anaerobic digestion material |
| CN115184441A (en) * | 2022-07-26 | 2022-10-14 | 山东艾科森特生物科技有限公司 | Online detection device and method for multi-channel enzyme electrode in fermentation production process |
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