CN219449706U - Online sterile sampling device of reactor - Google Patents
Online sterile sampling device of reactor Download PDFInfo
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- CN219449706U CN219449706U CN202320676690.7U CN202320676690U CN219449706U CN 219449706 U CN219449706 U CN 219449706U CN 202320676690 U CN202320676690 U CN 202320676690U CN 219449706 U CN219449706 U CN 219449706U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E30/00—Energy generation of nuclear origin
- Y02E30/30—Nuclear fission reactors
Abstract
The utility model discloses an online sterile sampling device for a reactor, and belongs to the technical field of sterile sampling. The device comprises a reactor, wherein the lower side wall of the reactor is connected with a sampling valve, the sampling valve is provided with two outlets, the two outlets are respectively connected with a first main pipeline and a second main pipeline, the first main pipeline is connected with a first branch pipeline and a second branch pipeline, the end part of the first branch pipeline is provided with a pure steam inlet, and the end part of the second branch pipeline is provided with a cleaning port; the second main pipeline is connected with a third branch pipeline and a fourth branch pipeline, the end part of the third branch pipeline is provided with a condensate water discharge port, and the end part of the fourth branch pipeline is provided with a waste discharge port. The sterilization temperature can be accurately controlled through the control system, the sequential switching steps of a plurality of valves are controlled by the control system, and the sterilization device is convenient, practical, high in working efficiency, capable of saving a large amount of manpower, avoiding human errors, capable of realizing online sterilization and capable of performing sterile sampling for a plurality of times.
Description
Technical Field
The utility model relates to an online sterile sampling device for a reactor, and belongs to the technical field of sterile sampling.
Background
In the course of cell culture or fermentation, it is necessary to sample the bioreactor or fermenter to obtain various important test data in order to achieve effective control of the individual reaction stages. When a sample is extracted from a bioreactor or a fermenter, the sampling device needs to be strictly sterilized to ensure the safe operation of the reaction process and the sterility of the operation process of the sampling device, thereby preventing the contamination of the materials in the bioreactor or the fermenter by the foreign bacteria introduced from the external environment. Currently, aseptic sampling is performed in a bioreactor or fermenter using a sampling device that can be sterilized on-line.
However, the existing aseptic sampling devices have the following disadvantages: 1. the sampling link is manually controlled by manpower, the sterilization temperature cannot be accurately controlled, the number of valves to be controlled is large, the steps are controlled sequentially, errors are easy to occur, the switch of each valve needs to be manually operated, and the working efficiency is low; 2. the on-line cleaning cannot be realized, the sampling valve is disassembled to perform off-line cleaning after the batch production is finished, and the cleaning cannot be performed in a sampling gap; 3. the sampling bottle cannot be replaced in the batch production process, and multiple sterile sampling cannot be performed.
Disclosure of Invention
In order to solve the problems, the utility model provides the reactor on-line sterile sampling device, which can accurately control the sterilization temperature through a control system, and the sequential switching steps of a plurality of valves are controlled by the control system.
The utility model aims to provide an online sterile sampling device of a reactor, which comprises the reactor, wherein the lower side wall of the reactor is connected with a sampling valve, the sampling valve is provided with two outlets, the two outlets are respectively connected with a first main pipeline and a second main pipeline, the first main pipeline is connected with a first branch pipeline and a second branch pipeline, the end part of the first branch pipeline is provided with a pure steam inlet, and the end part of the second branch pipeline is provided with a cleaning port; the second main pipeline is connected with a third branch pipeline and a fourth branch pipeline, the end part of the third branch pipeline is provided with a condensate water discharge port, and the end part of the fourth branch pipeline is provided with a waste discharge port.
In one embodiment of the utility model, the pure steam inlet is connected with a pure steam source, the cleaning port is connected with a cleaning pipeline, the condensed water discharge port is connected with a condensed water discharge pipeline, and the waste discharge port is connected with a waste discharge pipeline.
In one embodiment of the utility model, the first main pipeline is provided with a first manual valve, and the second main pipeline is connected with a second manual valve.
In one embodiment of the utility model, the first branch pipeline is provided with a first valve, and the second branch pipeline is provided with a second valve.
In one embodiment of the utility model, a third valve and a temperature detector are arranged on the second main pipeline, the temperature detector is used for detecting the temperature in the second main pipeline, and the temperature detector is positioned on the second main pipeline between the third valve and the third branch pipeline and the fourth branch pipeline.
In one embodiment of the utility model, a fourth valve and a drain valve are arranged on the third branch pipeline, and the drain valve is positioned between the fourth valve and the condensate water discharge port.
In one embodiment of the present utility model, a fifth valve is disposed on the fourth branch pipe.
In one embodiment of the present utility model, the system further comprises a control system, wherein the control system is in data connection with the first valve, the second valve, the third valve, the fourth valve, the fifth valve and the temperature detector.
In one embodiment of the present utility model, the first valve, the second valve, the third valve, the fourth valve and the fifth valve are pneumatic valves or electromagnetic valves.
In one embodiment of the utility model, one end of the second manual valve is connected with a second main pipeline between the third valve and the sampling valve, and the other end of the second manual valve is provided with a sampling port which is externally connected with a sampling bottle.
Advantageous effects
(1) The sterilization before sampling and the cleaning after sampling are controlled by the control system, the sterilization temperature can be accurately controlled by the control system, the sequential switching steps of a plurality of valves are controlled by the control system, and the sterilization device is convenient and practical, has high working efficiency, saves a large amount of manpower and avoids human errors.
(2) The utility model can automatically control the cleaning by the control system after the batch production is finished, and even can automatically clean the sample in the gap, thereby realizing real online cleaning and online sterilization.
(3) The utility model can carry out sterile sampling for a plurality of times in the batch production process, and can carry out sterile sampling again by automatically controlling the control system to sterilize after each time of replacing a new sterile sampling bottle.
Drawings
FIG. 1 is a schematic diagram of the reactor on-line aseptic sampling device of the present utility model.
In the figure: 1. a reactor; 2. a pure steam inlet; 3. a cleaning port; 4. a first main pipe; 5. a first branch pipe; 6. a second branch pipe; 7. a second main pipe; 8. a third branch pipe; 9. a fourth branch pipe; 10. a condensed water discharge port; 11. a waste discharge port; 12. a sampling valve; 13. a first manual valve; 14. a second manual valve; 15. a sampling port; 16. a temperature detector; 17. a drain valve; 18. a first valve; 19. a second valve; 20. a third valve; 21. a fourth valve; 22. and a fifth valve.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the present utility model, unless explicitly specified and limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
As shown in fig. 1, the embodiment provides an online sterile sampling device for a reactor, which comprises a reactor 1, wherein a sampling valve 12 is connected to the lower side wall of the reactor 1, the sampling valve 12 is provided with two outlets, the two outlets are respectively connected with a first main pipeline 4 and a second main pipeline 7, the first main pipeline 4 is connected with a first branch pipeline 5 and a second branch pipeline 6, the end part of the first branch pipeline 5 is provided with a pure steam inlet 2, and the end part of the second branch pipeline 6 is provided with a cleaning port 3; the second main pipeline 7 is connected with a third branch pipeline 8 and a fourth branch pipeline 9, a condensate water discharge port 10 is formed in the end portion of the third branch pipeline 8, and a waste discharge port 11 is formed in the end portion of the fourth branch pipeline 9.
Optionally, the pure steam inlet 2 is connected with a pure steam source, the cleaning port 3 is connected with a cleaning pipeline, the condensed water discharge port 10 is connected with a condensed water discharge pipeline, and the waste discharge port 11 is connected with a waste discharge pipeline.
Optionally, a first manual valve 13 is disposed on the first main pipe 4, and a second manual valve 14 is connected to the second main pipe 7.
Optionally, the first branch pipe 5 is provided with a first valve 18, and the second branch pipe 6 is provided with a second valve 19.
Optionally, a third valve 20 and a temperature detector 16 are arranged on the second main pipeline 7, the temperature detector 16 is used for detecting the temperature in the second main pipeline 7, and the temperature detector 16 is positioned on the second main pipeline 7 between the third valve 20 and the third branch pipeline 8 and the fourth branch pipeline 9; one end of the second manual valve 14 is connected with the second main pipeline 7 between the third valve 20 and the sampling valve 12, the other end of the second manual valve is provided with a sampling port 15, and the sampling port 15 is externally connected with a sampling bottle. When the second manual valve 14 is opened, the material in the reactor 1 flows out through the second manual valve 14 and the sampling port 15 to be sampled while flowing from the second main pipe 7 to the third valve 20.
Optionally, a fourth valve 21 and a drain valve 17 are provided on the third branch pipe 8, and the drain valve 17 is located between the fourth valve 21 and the condensate drain port 10.
Optionally, a fifth valve 22 is provided on the fourth branch pipe 9.
Optionally, a control system is also included, which is in data connection with the first valve 18, the second valve 19, the third valve 20, the fourth valve 21, the fifth valve 22 and the temperature detector 16. The indication of the temperature detector 16 may be read by the control system, or the opening and closing of the valve may be controlled by the control system.
Optionally, the first valve 18, the second valve 19, the third valve 20, the fourth valve 21 and the fifth valve 22 are pneumatic valves or solenoid valves, preferably pneumatic valves.
Alternatively, the material of the reactor 1 may be stainless steel, or other materials, which are not limited in particular.
The working principle of the utility model is as follows: when sampling is needed, the first manual valve 13 is kept in a closed state, the sampling valve 12 is opened, the material in the reactor 1 flows through the sampling valve 12 to the second manual valve 14 at the sampling port 15, the second manual valve 14 is opened, the material in the reactor 1 can be taken out, and after sampling, the sampling valve 12 is closed first, and then the second manual valve 14 is closed. Before the pipeline sterilization before sampling starts, the second manual valve 14 needs to be connected with the sampling bottle, sterilization is completed in the sterilization pot, and the second manual valve 14 is in a closed state after the sterilization is completed, so that the second manual valve 14 and the sampling bottle are in a sterile state.
On-line sterilization of the tubing prior to sampling: the sampling valve 12 and the second manual valve 14 are kept in a closed state, and the first manual valve 13 is opened. The control system opens the first valve 18 on the first branch pipe 5 connected to the source of pure steam, and simultaneously opens the third valve 20 on the second main pipe 7 and the fourth valve 21 on the third branch pipe 8. Pure steam flows through the valve cavity of the double-port sampling valve 12, flows to the third branch pipe 8 through the second main pipe 7, and when the steam passes through the drain valve 17 on the third branch pipe 8, gaseous steam is trapped, and water after steam condensation is released from the condensate water discharge port 10 to the condensate water discharge pipe. While the temperature detector 16 will detect the temperature downstream of the second mains 7 and feed it back to the control system. The control system determines the length of time for sterilization based on the indication of the temperature detector 16 and automatically closes all valves after sterilization is completed. After the temperature is naturally cooled, the sampling step is started.
Before each sampling, the pre-sterilized second manual valve 14 and the sampling bottle are connected, and after the pipeline is sterilized on line, the pipeline can be sampled. Multiple samples can be taken with such a cycle multiple times.
In addition, when the sampling valve 12 needs to be cleaned, the sampling valve 12 and the second manual valve 14 are kept in a closed state, the first manual valve 13 is opened, the control system opens the second valve 19 on the second branch pipeline 6, and cleaning water flows through the valve cavities of the double-port sampling valve 12 from the cleaning port 3, the second branch pipeline 6 and the first main pipeline 4 in sequence, so that the valve cavities in the sampling valve 12 are cleaned. The control system controls the third valve 20 and the fifth valve 22 to be opened, the fourth valve 21 is kept in a closed state, and the cleaned wastewater is discharged through the second main pipeline 7, the fourth branch pipeline 9 and the waste discharge port 11, so that the cleaning process is completed.
While the utility model has been described with reference to the preferred embodiments, it is not limited thereto, and various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the utility model as defined in the appended claims.
Claims (10)
1. The on-line sterile sampling device for the reactor is characterized by comprising the reactor, wherein the lower side wall of the reactor is connected with a sampling valve, the sampling valve is provided with two outlets, the two outlets are respectively connected with a first main pipeline and a second main pipeline, the first main pipeline is connected with a first branch pipeline and a second branch pipeline, the end part of the first branch pipeline is provided with a pure steam inlet, and the end part of the second branch pipeline is provided with a cleaning port; the second main pipeline is connected with a third branch pipeline and a fourth branch pipeline, the end part of the third branch pipeline is provided with a condensate water discharge port, and the end part of the fourth branch pipeline is provided with a waste discharge port.
2. The reactor on-line sterile sampling device of claim 1, wherein the pure steam inlet is connected to a pure steam source, the purge port is connected to a purge line, the condensate drain port is connected to a condensate drain line, and the waste drain port is connected to a waste drain line.
3. The reactor online sterile sampling device according to claim 1 or 2, wherein a first manual valve is arranged on the first main pipeline, and a second manual valve is connected to the second main pipeline.
4. An on-line sterile sampling device for a reactor according to claim 3, wherein the first branch conduit is provided with a first valve and the second branch conduit is provided with a second valve.
5. The reactor on-line aseptic sampling device of claim 4, wherein a third valve and a temperature detector are disposed on the second main conduit, the temperature detector is configured to detect a temperature in the second main conduit, and the temperature detector is disposed on the second main conduit between the third valve and the third and fourth branch conduits.
6. The reactor on-line aseptic sampling device of claim 5, wherein a fourth valve and a drain valve are disposed on the third branch conduit, the drain valve being located between the fourth valve and the condensate drain.
7. The reactor on-line aseptic sampling device of claim 6, wherein a fifth valve is provided on the fourth branch conduit.
8. The reactor online aseptic sampling device of claim 7, further comprising a control system in data connection with the first valve, second valve, third valve, fourth valve, fifth valve, and temperature detector.
9. The reactor online sterile sampling device according to claim 8, wherein the first, second, third, fourth and fifth valves are pneumatic or solenoid valves.
10. The reactor on-line aseptic sampling device of claim 5, wherein one end of the second manual valve is connected with a second main pipeline between the third valve and the sampling valve, and the other end is provided with a sampling port, and the sampling port is externally connected with a sampling bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320676690.7U CN219449706U (en) | 2023-03-30 | 2023-03-30 | Online sterile sampling device of reactor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320676690.7U CN219449706U (en) | 2023-03-30 | 2023-03-30 | Online sterile sampling device of reactor |
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| CN219449706U true CN219449706U (en) | 2023-08-01 |
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| CN202320676690.7U Active CN219449706U (en) | 2023-03-30 | 2023-03-30 | Online sterile sampling device of reactor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117402716A (en) * | 2023-09-27 | 2024-01-16 | 中国医学科学院医学生物学研究所 | Automatic sterilizing wastewater aseptic sampling device and working method thereof |
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2023
- 2023-03-30 CN CN202320676690.7U patent/CN219449706U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117402716A (en) * | 2023-09-27 | 2024-01-16 | 中国医学科学院医学生物学研究所 | Automatic sterilizing wastewater aseptic sampling device and working method thereof |
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