CN108548695A - A kind of automatic sampling system being suitable for medicine bioengineering reactor and its application - Google Patents
A kind of automatic sampling system being suitable for medicine bioengineering reactor and its application Download PDFInfo
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
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Abstract
The invention discloses a kind of automatic sampling systems being suitable for medicine bioengineering reactor, including detection components, control assembly, control system；The detection components include temperature sensor and pressure transmitter；The control assembly includes sampling bench, pneumatic diaphragm valve, manual diaphragm valve；The control system connects the detection components and the control assembly, the detection components send the data-signal detected to the control system, the data processing system of the control system handles the data-signal, the control system treated data signal transmission gives the control assembly, and controls the control assembly.The present invention collects detection, data processing and data and is transmitted as one, it can be according to multiple bioreactor difference sampling requirements, realize computer automation sampling control, effectively improve sampling efficiency and sampling quality, the success rate for improving biological sampling, the situation of fermentation field sampling can only be arrived by hand by changing medicine bioengineering reactor for a long time.
Description
Technical field
The invention belongs to technical field of pharmaceutical biotechnology, more particularly to a kind of automatic sampling being suitable for medicine bioengineering reactor
System and its application.
Background technology
In the production process of medicine bioengineering fermentation, biochemical sampling process is to correctly judging that biochemical process microbial status has
One of essential component part of important and production process.
However, large-scale Biopharmaceutical Enterprises still have laboratory employee to each bioreactor one by one into pedestrian at present
Work samples, and efficiency is very low, and easy tos produce microbial contamination, can meet high standard hygienic requirements there is an urgent need for a kind of and is suitable for curing
The automatic sampling system of medicine bioreactor.
Invention content
The present invention provides a kind of automatic sampling system being suitable for medicine bioengineering reactor, collection detection, data processing sum number
According to the automatic concentration sampling system that transmission is integrated, traditional sampling method is combined with industrial automation, focuses on pharmaceutical industry
The characteristics of high hygienic requirements, by the automatic valve on control system, sampling craft pipeline, to which system can be according to multiple reactions
Device difference sampling requirement realizes that computer automates sampling control simultaneously, is effectively improved sampling efficiency and sampling quality, carries
The success rate of high biological sampling, the situation of live hand sample can only be arrived by hand by changing medicine bioengineering for a long time.
Technical scheme is as follows：
A kind of automatic sampling system being suitable for medicine bioengineering reactor, including detection components, control assembly, control system
System；
The detection components include temperature sensor and pressure transmitter；
The control assembly includes sampling bench, pneumatic diaphragm valve, manual diaphragm valve；
The control system connects the detection components and the control assembly, the data that the detection components will detect
Signal sends the control system to, and the data processing system of the control system handles the data-signal, the control system
System treated data signal transmission gives the control assembly, and controls the control assembly.
Preferably, man-machine interface, sampling pipe, sampling bench manual diaphragm valve, sampling pipe are provided on the sampling bench
Terminal boring cover；Detection components and control assembly are passed through network connection to the control system by the man-machine interface.
Preferably, pneumatic sampling valve is provided on the side wall of bioreactor, the pneumatic sampling valve outlet end connection takes
Sample pipeline is connect with the sampling bench manual diaphragm valve on sampling bench, before the pneumatic sampling valve input end and a sampling valve
Pneumatic diaphragm valve connects；Main pipe rail is rinsed in one sterilization of pneumatic diaphragm valve input end connection before the sampling valve.
Preferably, the pneumatic sampling valve is set to the pot sidewall lower part that anti-biology answers device, and the control system is counted automatically
Calculate the pneumatic sampling valve opening time and filtrated air liftout time；To have the function of reducing material amounts, avoid
Bioreactor and concentration sampling bench are distant, sample the relatively large number of problem of inventory.
Preferably, it includes the hot water injection's pipeline, steam flush pipeline and nothing being arranged in parallel that main pipe rail is rinsed in the sterilization
Bacterium air douche pipeline is provided with hot water pneumatic diaphragm valve on hot water injection's pipeline, is arranged on the steam flush pipeline
There is steam pneumatic diaphragm valve, filtrated air pneumatic diaphragm valve is provided on the filtrated air flushing line；The sterilization is rinsed
Least significant end of the main pipe rail through bioreactor group is provided with sterilization and rinses main pipe rail blowdown pneumatic diaphragm valve and sterilization flushing manifold
Road terminal temperature sensor.
Preferably, the automatic sampling system is the concentration sampling system of multiple bioreactors, on bioreactor
The pneumatic sampling valve is connected by pipeline with the sampling bench, and described take is provided on the sampling pipe of the sampling bench
Sample platform manual diaphragm valve can screw the sampling pipe terminal boring cover by hand by sampling bench manual diaphragm valve outlet end.
Preferably, the man-machine interface is a live tablet computer, to operating personnel on the live tablet computer
Select tank number, sampling/mode of operation and confirmation operation.Operating personnel can select a certain sampling mode, select multiple biologies anti-
It answers device to be carried out at the same time sampling, different bioreactor difference sampling operation modes can also be selected to carry out while sampling operation, people
Optional mode of operation includes " full-automatic biochemical sampling ", " physics and chemistry sampling, " " simple sampling line sterilization ", " list on machine interface
Pure sampling line cleaning ".
Preferably, the set-up of control system is to be connect with biological reactor process process automatic control system.The control
System processed can be fully immersed into biological reactor process process master control system, passed through by cable access network based on ethernet interchanger
Other of interchanger and bioreactor automatic control system host computer are seamlessly connected to form biological medicine production management and control one
Body system.
Preferably, it is anti-in the fermentation tank, vaccine and monoclonal of biofermentation that the present invention also provides the automatic sampling systems
The application of the cell suspension cultures bioreactor of body use of large-scale production.
Compared with prior art, beneficial effects of the present invention are as follows：
The present invention collects detection, data processing and data and is transmitted as one, can be sampled and be wanted according to multiple bioreactor differences
It asks, realizes computer automation sampling control, effectively improve sampling efficiency and sampling quality, improve the success rate of biological sampling,
The situation of field sampling can only be arrived by hand by changing medicine bioengineering for a long time；
The pneumatic sampling valve of the present invention is set to the lower sidewall of bioreactor, by the way of lower sampling, need not take
Sample pipeline stretches into bioreactor, greatly reduces the contaminated risk of bioreactor, reduces the demand to detergent, both
The steam consumption that the water consumption and bioreactor sterilization of bioreactor cleaning can be lowered, can also meet the health of high standard
It is required that；
Certainly, it implements any of the products of the present invention and does not necessarily require achieving all the advantages described above at the same time.
Description of the drawings
Fig. 1 is the automatic sampling platform principle schematic of the embodiment of the present invention；
Fig. 2 is the sampling work principle schematic of the embodiment of the present invention；
Fig. 3 is the embodiment of the present invention bioreactor sampling work principle schematic；
Fig. 4 is the automatic sampling system topological diagram of the present invention；
Fig. 5 is the medicine bioengineering fermentation control integration system topological diagram of the present invention；
It is marked in figure：1- sampling bench；The scenes 2- tablet computer；3- hot water injection's indicator lights；4- wet sterilization indicator lights；5-
Indicator light can be sampled；6- sampling pipes；7- sampling bench manual diaphragm valves；8- sampling pipe terminal boring covers；9- sterilizations are rinsed total
Pipeline；Pneumatic diaphragm valve before 10- sampling valves；The pneumatic sampling valves of 11-；12- fermentation tank sampling line terminal temperature sensors；13-
Hot water pneumatic diaphragm valve；14- steam pneumatic diaphragm valves；15- filtrated air pneumatic diaphragm valves；16- sterilizes manifold pressure transmitter；
Main pipe rail temperature sensor is rinsed in 17- sterilizations；Main pipe rail blowdown pneumatic diaphragm valve is rinsed in 18- sterilizations；Main pipe rail is rinsed in 19- sterilizations
Terminal temperature sensor；20- fermentation control system host computers；21- printers；22- interchangers；23-DCS control systems.
Specific implementation mode
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate this hair
It is bright, rather than limit protection scope of the present invention.Those skilled in the art change according to what the present invention made in practical applications
Into and adjustment, still fall within protection scope of the present invention.
In order to better illustrate the present invention, the present invention is described in detail below with attached drawing.
As shown in Figure 1, Figure 2 and Figure 3, a kind of automatic sampling system being suitable for medicine bioengineering reactor, including detection group
Part, control assembly, control system；
The detection components include temperature sensor and pressure transmitter；
The control assembly includes sampling bench, pneumatic diaphragm valve, manual diaphragm valve；
The control system connects the detection components and the control assembly, the data that the detection components will detect
Signal sends the control system to, and the data processing system of the control system handles the data-signal, the control system
System treated data signal transmission gives the control assembly, and controls the control assembly.
Specifically, being provided with man-machine interface, sampling pipe 6, sampling bench manual diaphragm valve 7, probe tube on the sampling bench 1
Road terminal boring cover 8；Detection components and control assembly are passed through network connection to the control system by the man-machine interface.
Pneumatic sampling valve 11 is provided on bioreactor side wall, 11 outlet end of pneumatic sampling valve connects probe tube
Road is connect with the sampling bench manual diaphragm valve 7 on sampling bench 1, before 11 input end of pneumatic sampling valve and a sampling valve
Pneumatic diaphragm valve 10 connects；Main pipe rail 9 is rinsed in 10 input end of pneumatic diaphragm valve connection, one sterilization before the sampling valve.
Further, the pneumatic sampling valve 11 is set to bioreactor lower sidewall, and the control system is counted automatically
Calculate 11 opening time of pneumatic sampling valve and filtrated air liftout time；To have the function of reducing material amounts, avoid
It bioreactor and concentrates sampling bench 1 distant, samples inventory relatively large number of problem.
Further, it is described sterilization rinse main pipe rail 9 include be arranged in parallel hot water injection's pipeline, steam flush pipeline and
Filtrated air flushing line, is provided with hot water pneumatic diaphragm valve 13 on hot water injection's pipeline, on the steam flush pipeline
It is provided with steam pneumatic diaphragm valve 14, filtrated air pneumatic diaphragm valve 15 is provided on the filtrated air flushing line；It is described
Sterilization rinses end of the main pipe rail 9 after bioreactor tank battery and is provided with 18 He of sterilization flushing main pipe rail blowdown pneumatic diaphragm valve
Main pipe rail terminal temperature sensor 19 is rinsed in sterilization.Optionally, as needed, several indicator lights are provided on the sampling bench 1,
Each bioreactor is correspondingly arranged on hot water injection's indicator light 3, wet sterilization indicator light 4, can sample indicator light 5, to divide
Unlatching/closed state of hot water pneumatic diaphragm valve 13, steam pneumatic diaphragm valve 14, filtrated air pneumatic diaphragm valve 15 is not shown,
Sampling process is better understood by indicator light.
Further, the automatic sampling system is set as the concentration sampling of multiple bioreactors, on bioreactor
The pneumatic sampling valve 11 be connected with the sampling bench 1 by pipeline, be arranged on the sampling pipe 6 of the sampling bench 1
There is the sampling bench manual diaphragm valve 7, the sampling pipe can be screwed by 7 outlet end of sampling bench manual diaphragm valve by hand
Terminal boring cover 8.It suspends it is appreciated that the automatic sampling system is set as multiple fermentation tanks, multiple seeding tanks or multiple cells
Cultivate the concentration sampling of the reactor of biology.
Further, the man-machine interface is a live tablet computer 2, to operating personnel in the live tablet computer
Tank number, sampling/mode of operation and confirmation operation are selected on 2.Operating personnel can select a certain sampling mode, select multiple biologies
Reactor is carried out at the same time sampling, and the different sampling operation modes of different bioreactors can also be selected to be sampled behaviour simultaneously
Make, optional mode of operation includes " full-automatic biochemical sampling " in man-machine interface, " physics and chemistry sampling, " " simple sampling line kills
Bacterium ", " simple sampling line cleaning ".
Further, the set-up of control system is to be connect with biological reactor process process automatic control system.It is described
Control system can be fully immersed into other master control systems, pass through interchanger and other by cable access network based on ethernet interchanger
Automatic control system host computer is seamlessly connected to form biological medicine production control integration system.Described in it is appreciated that certainly
Autocontrol system is set as connecting with ferment tank automatic control system or the automatic control system is set as outstanding with cell
The reactor reaction control system connection of floating culture biology.
The automatic sampling system is thin the fermentation tank of biofermentation, vaccine and monoclonal antibody use of large-scale production
The application of born of the same parents' suspension culture bioreactors can meet biofermentation fermentation tank, vaccine and monoclonal antibody large-scale production
High standard hygienic requirements of the cell suspension cultures bioreactor used to sampling system.
The present invention is described in detail with reference to specific embodiments of the present invention.
Embodiment
As shown in Figure 1, Figure 2 and Figure 3, a kind of automatic sampling system being suitable for medicine bioengineering, including a sampling bench 1 and with
The pneumatic sampling valve 11 that fermentation tank mutually welds, pneumatic diaphragm valve 10 before sampling valve；
Concentrated setting has live tablet computer 2, sampling pipe 6, sampling bench hand-operated valve, sampling pipe terminal on sampling bench 1
Boring cover 8, each fermentation tank sampling line terminal temperature sensor 12；Optionally, sampling bench is stainless steel sampling bench；
Component on fermentation tank includes pneumatic diaphragm valve 10 before pneumatic sampling valve 11, sampling valve.
Component includes hot water pneumatic diaphragm valve 13, steam pneumatic diaphragm on its sterilization cleaning manifold of the automatic sampling system
Valve 14, filtrated air pneumatic diaphragm valve 15, sterilization manifold pressure transmitter 16, sterilization are rinsed main pipe rail temperature sensor 17, are killed
Bacterium rinses main pipe rail blowdown pneumatic diaphragm valve 18, main pipe rail terminal temperature sensor 19 is rinsed in sterilization and the connection of these equipment
Pipeline；Be suitable in the present embodiment the valve of the automatic sampling system of medicine bioengineering fermentation using pneumatic sampling valve and hygiene-type every
Film valve also can use pneumatic ball valve, the present invention not to be limited according to the height of product hygienic requirements certainly.
As shown in figure 4, the present invention includes Data Detection, input, processing and transmission process.Live tablet computer 2 is for true
Recognize, flow for displaying picture and process data.Live tablet computer 2 is used as execute-in-place unit, and can individually and control system
Connection, becomes single automation sampling system；Optionally, set-up of control system of the invention is DCS control systems 23.
As shown in figure 5, single automation sampling subsystem can completely be melted by cable access network based on ethernet interchanger 22
Enter the master control system that ferments, medicine bioengineering fermentation management and control one is formed by interchanger 22 and fermentation control system host computer 20
Body system.Optionally, printer 21 can also be connected.
Operating personnel 2 selection tanks number and sampling/mode of operation and confirmation operation on tablet computer at the scene.Certainly with fermentation tank
When autocontrol system is combined into one, the scene tablet computer 2 can also be opened mandate if necessary, and operating personnel can
To carry out fermentation operation on the live tablet computer 2 of sampling bench.
The course of work of the present invention
One, cleaning program is initialized
When all setting completely, needs to clean the progress such as manifold and its valve being connected to sampling line, sterilization equipment
Cleaning, it is ensured that without sundries such as welding slags inside pipeline.
Specific method
The manually opened stainless steel of step 1 concentrates all sampling bench manual diaphragm valves 7 on sampling bench 1, unscrewing sampling bench manual
Sampling pipe terminal boring cover 8 on diaphragm valve 7；
Step 2 opens sterilization and rinses main pipe rail blowdown pneumatic diaphragm valve 18, opens hot water pneumatic diaphragm valve 13, so first
Manifold hot water injections are rinsed to sterilization, rinsing main pipe rail terminal temperature sensor 19 when sterilization detects that temperature reaches 85 degree and starts
It counts, rinses a period of time, it is assumed that can be set as 3min；
Pneumatic diaphragm valve 10 before the sampling valve of step 3 first fermentation tank of unlatching, closes sterilization and rinses main pipe rail blowdown gas
Dynamic diaphragm valve 18, hot water are flowed out from the sampling pipe of first fermentation tank, and fermentation tank sampling line terminal temperature sensor 12 is surveyed
85 degree are reached to temperature to start counting up, and rinse a period of time, it is assumed that can be set as 5min；In flushing process crawl open and
Close pneumatic sampling valve 11 so that the sampling head of pneumatic sampling valve 11 is also cleaned；
First fermentation tank sampling pipe hot water scavenging period of step 4 arrives, then before the sampling valve for opening second fermentation tank
Pneumatic diaphragm valve 10 closes pneumatic diaphragm valve 10 before the sampling valve of first fermentation tank, sampling of the hot water from second fermentation tank
Pipeline flows out, and fermentation tank sampling line terminal temperature sensor 12 measures 85 degree of temperature arrival and starts counting up, and rinses a period of time,
Assuming that can be set as 5min；Crawl opens and closes pneumatic sampling valve 11 in flushing process so that pneumatic sampling valve 11
Sampling head is also cleaned；And so on, the sampling line of all fermentation tanks is cleaned one time with hot water；
Step 5 closes hot water pneumatic diaphragm valve 13, opens sterilization and rinses main pipe rail blowdown pneumatic diaphragm valve 18, closes last
Pneumatic diaphragm valve 10 before the sampling valve of the fermentation tank of cleaning；Discharge sterilization rinses the hot water in main pipe rail for a period of time；It opens and steams
Vapour pneumatic diaphragm valve 14, it is assumed that sterilization is set as 10min；Main pipe rail terminal temperature sensor 19, which is rinsed, when sterilization detects temperature
It reaches 100 degree to start counting up, the wet sterilization time that manifold is rinsed in sterilization arrives, then opens gas before the sampling valve of first fermentation tank
Dynamic diaphragm valve 10 closes sterilization and rinses main pipe rail blowdown pneumatic diaphragm valve 18, sampling pipe stream of the steam from first fermentation tank
Cross, fermentation tank sampling line terminal temperature sensor 12 measure temperature reach 100 degree start counting up, it is assumed that set sterilizing time as
10min, the time is to then crawl opens and closes pneumatic sampling valve 11 in flushing process so that the sampling head of pneumatic sampling valve 11
Also it is sterilized；
The first fermentation tank sampling pipe wet sterilization time of step 6 arrives, then before the sampling valve for opening second fermentation tank
Pneumatic diaphragm valve 10 closes pneumatic diaphragm valve 10 before the sampling valve of first fermentation tank, sampling of the steam from second fermentation tank
Pipeline flows out, and fermentation tank sampling line terminal temperature sensor 12 measures 100 degree of temperature arrival and starts counting up, and sterilizing time can be set
It is set to 10min；Crawl opens and closes pneumatic sampling valve 11 in sterilization process so that the sampling head of pneumatic sampling valve 11 also obtains
To cleaning；Sterilizing time is to the sterilization for then going to next fermentation tank；Operating personnel close the fermentation tank for just terminating sterilization at this time
Sampling bench manual diaphragm valve 7, screw sampling pipe terminal boring cover 8；And so on, until by the sampling line of all fermentation tanks
With wet sterilization one time；
Step 7 terminates when the sterilization of the last one fermentation tank sampling line, and steam off pneumatic diaphragm valve 14, closing is finally killed
Pneumatic diaphragm valve 10 before the sampling valve of the fermentation tank of bacterium；Operating personnel close just terminate sterilization fermentation tank sampling bench manually every
Film valve 7 screws sampling pipe terminal boring cover 8.
Two, the biochemical sampling method of automatic sampling system
Step 1 operating personnel concentrate 1 manually opened sampling bench manual diaphragm valve 7 of sampling bench in stainless steel, unscrew probe tube
Road terminal boring cover 8；
Operating personnel click " tank number selection " button to step 2 on tablet computer 2 at the scene, pass through pull-down menu selection need
The tank number to be operated；Clicking operation requires button, and such as " full-automatic biochemical sampling ", " physics and chemistry sampling, " " simple sampling line kills
Bacterium ", " simple sampling line cleaning " button, here we click " full-automatic biochemical sampling " button；
Step 3 clicks " startup " button, and the require information of operating personnel is transmitted DCS control systems by live tablet computer 2
23；
Step 4 robot control system(RCS) starts to automate sampling operation, by taking first fermentation tank carries out full-automatic biochemical sampling as an example；
System automatically turns on sterilization and rinses main pipe rail blowdown pneumatic diaphragm valve 18, opens steam pneumatic diaphragm valve 14, at this time stainless steel collection
Bright 4 of wet sterilization indicator light on middle sampling bench 1 prompts the current automatic running flow of operating personnel；Assuming that manifold sterilizations are set
It is set to 2min, rinsing main pipe rail terminal temperature sensor 19 when sterilization detects that temperature reaches 100 degree and starts counting up, and sterilization is rinsed
The wet sterilization time of manifold arrives, then opens pneumatic diaphragm valve 10 before the sampling valve of first fermentation tank, closes sterilization and rinses always
Pipeline blowdown pneumatic diaphragm valve 18, steam is flowed through from the sampling pipe of first fermentation tank, fermentation tank sampling line terminal temperature
Sensor 12 measures 100 degree of temperature arrival and starts counting up；
Step 5 sets sampling pipe sterilizing time as 10min, and gate time arrives, then steam off pneumatic diaphragm valve
14, open filtrated air pneumatic diaphragm valve 15, with filtrated air start to sampling line purge steam condensed water while, reduce
The temperature of sampling pipe, when fermentation tank sampling line terminal temperature sensor 12 detects that temperature less than 30 degree, closes sterile sky
Gas pneumatic diaphragm valve 15 closes pneumatic diaphragm valve 10 before sampling valve；
Step 6 wet sterilization indicator light extinguishes 4, can sample the flicker of indicator light 5 and light, remind operating personnel's sampling, this
When, operating personnel are connected on conical flask is sampled under sampling bench manual diaphragm valve 7, until sampling zymotic fluid；
Step 7 is automatically controlled opens pneumatic sampling valve automatically according to the executeaaafunction value the time required to obtaining normal sampling amount
After 11, pneumatic sampling valve 11 is closed, opens pneumatic diaphragm valve 10 before sampling valve, crawl opens filtrated air pneumatic diaphragm valve 15
2~3 times so that sterilization rinses main pipe rail 9 and carries certain minute-pressure so that the zymotic fluid indentation sampling conical flask sampled；
After the zymotic fluid sampled needed for step 8 enters sampling conical flask, operating personnel can tampon beyond the Great Wall；
Step 9 at this time hot water injection's indicator light 3 grow it is bright, control system automatically turn on hot water pneumatic diaphragm valve 13 to probe tube
Road hot water injection；
Step 10 terminates as fermentation tank sampling line hot water injection, closes the fermentation tank of hot water pneumatic diaphragm valve 13 and sterilization
Sampling valve before pneumatic diaphragm valve 10；Hot water injection's indicator light 3 extinguishes, and operating personnel close taking for the fermentation tank for just terminating sterilization
Sample platform manual diaphragm valve 7 screws sampling pipe terminal boring cover 8；Sampling conical flask can be held and carry out microorganism to laboratory
Analysis.
Three, the physics and chemistry sampling method of automatic sampling system
Step 1 operating personnel concentrate 1 manually opened sampling bench manual diaphragm valve 7 of sampling bench in stainless steel, unscrew probe tube
Road terminal boring cover 8；
Operating personnel click " tank number selection " button to step 2 on tablet computer 2 at the scene, pass through pull-down menu selection need
The tank number to be operated, clicking operation " physics and chemistry sampling ", button；
Step 3 clicks " startup " button, and the require information of operating personnel is transmitted DCS control systems by live tablet computer 2
23；
Step 4 at this time light by hot water injection's indicator light 3, and robot control system(RCS) automatically turns on hot water pneumatic diaphragm valve 13 to probe tube
Road hot water injection；
Step 5 sets sampling pipe hot water injection's time as 2min, and gate time arrives, then closes hot water pneumatic diaphragm
Valve 13 opens filtrated air pneumatic diaphragm valve 15, while starting to purge hot water to sampling line with filtrated air, reduces sampling
The temperature of pipeline, when fermentation tank sampling line terminal temperature sensor 12 detects that temperature less than 30 degree, closes filtrated air gas
Dynamic diaphragm valve 15 closes pneumatic diaphragm valve 10 before sampling valve；
Step 6 hot water injection indicator light 3 extinguishes, and can sample the flicker of indicator light 5 and light, remind operating personnel's sampling, this
When, operating personnel are connected on conical flask is sampled under sampling bench manual diaphragm valve 7, until sampling zymotic fluid；
Step 7 is automatically controlled opens pneumatic sampling valve automatically according to the executeaaafunction value the time required to obtaining normal sampling amount
After 11, pneumatic sampling valve 11 is closed, opens pneumatic diaphragm valve 10 before sampling valve, crawl opens filtrated air pneumatic diaphragm valve 15
2~3 times so that sterilization rinses main pipe rail 9 and carries certain minute-pressure so that the zymotic fluid indentation sampling conical flask sampled；
After the zymotic fluid sampled needed for step 8 enters sampling conical flask, operating personnel can tampon beyond the Great Wall；
Step 9 at this time hot water injection's indicator light 3 grow it is bright, robot control system(RCS) automatically turn on hot water pneumatic diaphragm valve 13 to probe tube
Road hot water injection；
Step 10 terminates as fermentation tank sampling line hot water injection, closes the fermentation tank of hot water pneumatic diaphragm valve 13 and sterilization
Sampling valve before pneumatic diaphragm valve 10；Hot water injection's indicator light 3 extinguishes, and operating personnel close taking for the fermentation tank for just terminating sterilization
Sample platform manual diaphragm valve 7 screws sampling pipe terminal boring cover 8, can hold sampling conical flask and carry out microorganism to laboratory
Analysis.
Medicine bioengineering ferments control integration system can be with each workshop of full factory, such as extraction workshop, solvent recovery workshop, storage
The robot control system(RCS) in the workshops such as tank field organically combines, and becomes a part for full factory's synthesis robot control system(RCS).Fermentation plant Redundant Ethernet
Other substations of interchanger and host computer are connected to form entirety with optical fiber.Host computer not only have check tank area,
Solvent tank field, the function of extracting workshop situation also have and receive the data that other inter-vehicle communications come, handle it, analyze and send out
Send function.
Tablet computer 2 and fermentation control system host computer 20 can check extraction workshop tank, solvent recycling at the scene
Workshop tank, tank area current storage capacity situation, it is contemplated that the difference of workshop property, live tablet computer 2 and ferment control system
System host computer 20 cannot interfere, the operation of the valve, pump that manipulate other workshops.
Above-described embodiment is the application in the fermentation tank sampling of biofermentation, certainly also can be by above-mentioned automatic sampling system
Applied to the automatic sampling of the cell suspension cultures bioreactor used in vaccine and monoclonal antibody large-scale production, also may be used
Automatic sampling applied to other biological reactor.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to preferably explain the present invention
Principle and practical application, to enable skilled artisan to be best understood by and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (9)
1. a kind of automatic sampling system being suitable for medicine bioengineering reactor, which is characterized in that including detection components, control group
Part, control system；
The detection components include temperature sensor and pressure transmitter；
The control assembly includes sampling bench, pneumatic diaphragm valve, manual diaphragm valve；
The control system connects the detection components and the control assembly, the data-signal that the detection components will detect
Send the control system to, the data processing system of the control system handles the data-signal, at the control system
Data signal transmission after reason gives the control assembly, and controls the control assembly.
2. automatic sampling system according to claim 1, which is characterized in that be provided on the sampling bench man-machine interface,
Sampling pipe, sampling bench manual diaphragm valve, sampling pipe terminal boring cover；The man-machine interface requires operation to pass through network transmission
To the control system, the control system is judged according to the data that the detection components detect, the control system
It controls the control assembly and executes automation sampling operation.
3. automatic sampling system according to claim 2, which is characterized in that be provided on the side wall of bioreactor pneumatic
Sampling valve, the pneumatic sampling valve outlet end connect sampling line, are connect with the sampling bench manual diaphragm valve on sampling bench,
The pneumatic sampling valve input end is connect with pneumatic diaphragm valve before a sampling valve；Pneumatic diaphragm valve input end connects before the sampling valve
It connects a sterilization and rinses main pipe rail.
4. automatic sampling system according to claim 3, which is characterized in that the pneumatic sampling valve is set to biological respinse
Device lower sidewall, the control system calculate the pneumatic sampling valve opening time and filtrated air liftout time automatically.
5. automatic sampling system according to claim 3, which is characterized in that the sterilization is rinsed main pipe rail and set including parallel connection
Hot water injection's pipeline, steam flush pipeline and the filtrated air flushing line set are provided with hot water on hot water injection's pipeline
Pneumatic diaphragm valve is provided with steam pneumatic diaphragm valve on the steam flush pipeline, is arranged on the filtrated air flushing line
There is filtrated air pneumatic diaphragm valve；The sterilization rinses least significant end of the main pipe rail through bioreactor group and is provided with sterilization flushing always
Main pipe rail terminal temperature sensor is rinsed in pipeline blowdown pneumatic diaphragm valve and sterilization.
6. automatic sampling system according to claim 3, which is characterized in that the automatic sampling system is that multiple biologies are anti-
The concentration sampling system of device is answered, the pneumatic sampling valve on bioreactor is connected by pipeline with the sampling bench, described
It is provided with the sampling bench manual diaphragm valve on the sampling pipe of sampling bench, passes through the sampling bench manual diaphragm valve outlet
End can screw the sampling pipe terminal boring cover by hand.
7. automatic sampling system according to claim 1, which is characterized in that the man-machine interface is a live tablet electricity
Brain selects tank number, sampling/mode of operation and confirmation operation to operating personnel on the live tablet computer.
8. automatic sampling system according to claim 1, which is characterized in that the set-up of control system is and biological respinse
Device technical process automatic control system connects.
9. claim 1-8 any one of them automatic sampling system is in the fermentation tank, vaccine and monoclonal antibody of biofermentation
The application of the cell suspension cultures bioreactor of use of large-scale production.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810136410.7A CN108548695A (en) | 2018-02-09 | 2018-02-09 | A kind of automatic sampling system being suitable for medicine bioengineering reactor and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810136410.7A CN108548695A (en) | 2018-02-09 | 2018-02-09 | A kind of automatic sampling system being suitable for medicine bioengineering reactor and its application |
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| CN110455586A (en) * | 2019-08-30 | 2019-11-15 | 英诺维尔智能科技（苏州）有限公司 | A kind of continuous sampling method suitable for the production of scale biological products |
| WO2020238769A1 (en) * | 2019-05-24 | 2020-12-03 | 南京金斯瑞生物科技有限公司 | Biological reaction apparatus and method for performing biological detection on basis of apparatus |
| GB2609490A (en) * | 2021-08-05 | 2023-02-08 | Oribiotech Ltd | Outlet valve for a container |
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Application publication date: 20180918 |