CN205333518U - Making wine zymotic fluid yeast vigor on -line measuring device based on raman spectroscopy - Google Patents
Making wine zymotic fluid yeast vigor on -line measuring device based on raman spectroscopy Download PDFInfo
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- CN205333518U CN205333518U CN201620031217.3U CN201620031217U CN205333518U CN 205333518 U CN205333518 U CN 205333518U CN 201620031217 U CN201620031217 U CN 201620031217U CN 205333518 U CN205333518 U CN 205333518U
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Abstract
The utility model discloses a making wine zymotic fluid yeast vigor on -line measuring device based on raman spectroscopy, including pump, sample cell, three -way valve, flow -through cell, laser instrument, optic fibre, raman probe, speculum, spectrum appearance, computer, the laser instrument is popped one's head in in the raman through the fiber connection, the head of raman probe is connected with the first surface of sample cell, the speculum is located on the second surface of sample cell, the first surface is parallel to each other with the second surface, the spectrum appearance passes through communication interface and connects in the computer, the sample cell is made by transparent material. The utility model discloses realize among the brewing process that zymotic fluid yeast vigor carries out on -line measuring, make distillery and optimize fermentation process and have an important meaning instructing, the utility model discloses can measure zymotic fluid yeast vigor accurate, fast.
Description
Technical field
This utility model relates to the Detection & Controling field of brewing fermentation process, particularly to the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum。
Background technology
Fermentation is one of link of mostly important in wine brewing process, most critical, and the quality of fermentation liquid yeast quality is directly connected to that the yield of its tunning is how many and quality。The quality of yeast is generally evaluated by activity and vigor, and activity is the viability of phalangeal cell, and yeast activity refers to the physiological status of yeast cells, it is possible to be interpreted as that living cells is to the adaptive capacity of environment and metabolic capacity。Yeast activity is the key factor affecting fermenting property and taste, yeast only stable, high vigor, just can brew high-quality wine。Therefore, how to realize the on-line checking to fermentation liquid yeast activity, be always up the emphasis that liquor industry is concerned about and is studied。
Traditional yeast activity measures mainly blood counting chamber culture method, vigor titrimetry, acidifying amylograph, the absorption process of oxygen, CO2Method for releasing, magnesium ion method for releasing, methylene blue reducing process etc.。But all there is some defects and deficiency in these methods, as sensitivity is low；Most assay methods are off-line measurement, may introduce some miscellaneous bacterias, affect measurement result in measurement process；The detection time is longer, causes that the feedback information obtained relatively lags behind, it is impossible to reflect the change of yeast activity in time, thus the monitor in real time that impact is to sweat；Sometimes also need to add some fluorescent dyes, be highly detrimental to the on-line checking of production process。
In brewing fermentation field, due to the complexity and diversity of fermentation broth contents, develop the device for fermentation broth contents detection at present considerably less, it does not have a kind of device can realize the on-line checking of fermentation liquid yeast activity in brewing fermentation process。
Raman spectrum is as a kind of spectral detection means received significant attention in recent years; there is quick, efficient, nondestructive analysis, without pre-treatment, the advantage such as pollution-free, be successfully applied to the fields such as petrochemical industry, medical science, pharmacy, environmental conservation, archaeology qualification, food safety。But this method is so far all without the quantitative analysis being applied to yeast activity, more not used for the on-line checking of fermentation liquid yeast activity in wine brewing process。
Utility model content
In order to overcome disadvantages mentioned above and the deficiency of prior art, the purpose of this utility model is in that to provide a kind of brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum, fermentation liquid yeast activity in wine brewing process can be carried out on-line checking, fermentation liquid yeast activity can be measured accurately and rapidly。
The purpose of this utility model is achieved through the following technical solutions:
Based on the brewing fermentation liquid yeast activity on-line measuring device of Raman spectrum, including pump, sample cell, three-way valve, flow cell, laser instrument, optical fiber, Raman probe, reflecting mirror, spectrogrph, computer；
The arrival end of described pump is connected with the sample tap of fermentation tank, and delivery side of pump end is connected with the injection port of sample cell；The outlet of described sample cell is connected with the import of three-way valve, and two outlets of described three-way valve are connected with fermentation tank and flow cell respectively；
Described laser instrument is connected to Raman probe by optical fiber, and the head of described Raman probe is connected with the first surface of sample cell, and described reflecting mirror is located on the second surface of described sample cell, and described first surface and second surface are parallel to each other；Described spectrogrph is connected to computer by communication interface；Described sample cell is made of clear material；
Described Raman probe is connected with spectrogrph by optical fiber。
The described brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum, also includes display；Described computer is connected to display by COM1。
Described laser instrument is visible laser or infrared light laser instrument。
Described Raman probe is the Raman probe of focus adjustable。
Described sample cell is made up of quartz material。
Based on the brewing fermentation liquid yeast activity online test method of Raman spectrum, comprise the following steps:
(1) opening pump, three-way valve leads to flow cell, and fermentation liquid to be measured is introduced sample cell；
(2) after sample cell fills fermentation liquid gradually, open laser instrument, gathered the Raman spectrum data of fermentation liquid by Raman probe；After Raman spectrum data is carried out denoising, smooth, baseline correction, extract the characteristic spectrum of nucleic acid, protein and lipid based on successive projection algorithm；
(3) fermentation liquid in sample cell circulates pond through three-way valve, collects training sample, measure fermentation liquid yeast activity in flow cell by the method for vigor titration in flow cell；
(4) with the intensity at nucleic acid, protein and lipid profile peak for independent variable, the fermentation liquid yeast activity measured with the method for vigor titration, for dependent variable, sets up the Quantitative Analysis Model of yeast activity and nucleic acid, protein and lipid profile peak intensity with chemometrics method；
(5) three-way valve leads to fermentation tank, fermentation liquid circulates between fermentation tank and sample cell, Raman spectrum data by Raman probe Real-time Collection fermentation liquid, after Raman spectrum data is carried out denoising, smooth, baseline correction, extract the characteristic spectrum of nucleic acid, protein and lipid based on successive projection algorithm；
(6) the prediction yeast activity of the Quantitative Analysis Model described in characteristic spectrum step (4) of nucleic acid, protein and the lipid that step (5) is obtained。
The described brewing fermentation liquid yeast activity online test method based on Raman spectrum, carries out following steps after step (6):
(7) predicting the outcome of step (6) is shown over the display。
Principle of the present utility model is:
Certain proportionate relationship is there is in the raman scattering intensity that the basis of Raman spectrum quantitative analysis is test analyte with its concentration。And the change of the characteristic peak that the change of fermentation liquid yeast activity is relevant to its nucleic acid, protein and lipid is directly related in brewing fermentation process。Therefore, it can, by Raman spectrum on-line measuring device, set up Quantitative Analysis Model in conjunction with chemometrics method, it is achieved to the on-line checking of fermentation liquid yeast activity in wine brewing process。
Compared with prior art, this utility model has the following advantages and beneficial effect:
(1) device of the present utility model is airtight, circulation, so both can avoid other microorganisms pollution to yeast, and can avoid again the wasting of resources (except training sample collection)。
(2) this utility model can replace conventional offline detection method, it is achieved the on-line checking of fermentation liquid yeast activity。
(3) test process of the present utility model need not destroy sample, contact sample, it is not necessary to adds any fluorescent dye, belongs to " green " analytical technology。
(4) apparatus structure of the present utility model is compact, and volume is little, and device is applicable not only to brewing industry and produces, and is also applied for laboratory room small-sized fermenting experiment。
(5) device of the present utility model is safeguarded fairly simple, it is possible to long time continuous working。
(6) in actual production process, it is possible to constantly update training sample, to guarantee accuracy and the robustness of calibration model。
Accompanying drawing explanation
Fig. 1 is this utility model schematic diagram based on the brewing fermentation liquid yeast activity on-line measuring device of Raman spectrum。
Fig. 2 is this utility model flow chart based on the brewing fermentation liquid yeast activity online test method of Raman spectrum。
Detailed description of the invention
Below in conjunction with embodiment, this utility model is described in further detail, but embodiment of the present utility model is not limited to this。
Embodiment
The brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum of the present embodiment, including pump 3, sample cell 4, three-way valve 5, flow cell 6, laser instrument 7, optical fiber 8, Raman probe 9, reflecting mirror 10, spectrogrph 11, computer 12 and display 13。
The arrival end of described pump 3 is connected with fermentation tank 1 (built-in fermentation liquid 2) sample tap, the port of export of pump 3 is connected with the injection port of sample cell 4, the outlet of described sample cell 4 is connected with the import of three-way valve 5, two outlets of described three-way valve 5 are connected with fermentation tank 1 and flow cell 6 respectively, and described flow cell 6 is used for placing treats fermentation liquid that conventional method is measured or for discharging the fermentation liquid being trapped in sample cell；
Described laser instrument 7 is connected with Raman probe 9 by optical fiber 8, and the head of described Raman probe 9 is connected with the first surface of sample cell 4, and described reflecting mirror 10 is located on the second surface of described sample cell 4, and described first surface and second surface are parallel to each other；Sample cell 4 is used for placing the fermentation liquid treating Raman spectroscopy scans；
Described Raman probe 9 is connected to spectrogrph 11 by another optical fiber 8, described spectrogrph 11 is connected to computer 12 by communication interface, described computer 12 is connected to display 13 by COM1, and described display 13 is for the display of fermentation liquid yeast activity level。
In the present embodiment, laser instrument is semiconductor laser, and output light is visible or infrared light。The focal length of described Raman probe can regulate, it is ensured that during measurement, light beam can accurately focus on measured zone；Described display is light-emitting diode display。
The brewing fermentation liquid yeast activity online test method based on Raman spectrum of the present embodiment, comprises the following steps:
(1) opening pump, three-way valve leads to flow cell, and fermentation liquid to be measured slowly introduces sample cell；
(2) after sample cell fills fermentation liquid gradually, open laser instrument, the Raman spectrum data of fermentation liquid is gathered by Raman probe, by software WIRE3.3, Raman spectrum initial data is carried out the pretreatment such as denoising, smooth, baseline correction, extract the characteristic spectrum relevant to nucleic acid, protein and lipid based on successive projection algorithm；
(3) fermentation liquid in sample cell circulates pond through three-way valve, collects a number of training sample in flow cell, measures fermentation liquid yeast activity in flow cell by the method for vigor titration；
(4) with the intensity at nucleic acid, protein and lipid profile peak for independent variable, the fermentation liquid yeast activity measured with the method for vigor titration, for dependent variable, sets up the Quantitative Analysis Model of yeast activity and nucleic acid, protein and lipid profile peak intensity with chemometrics method；
(5) three-way valve leads to fermentation tank, fermentation liquid circulates between fermentation tank and sample cell, Raman spectrum data by Raman probe Real-time Collection fermentation liquid, after Raman spectrum data is carried out denoising, smooth, baseline correction, extract the characteristic spectrum of nucleic acid, protein and lipid based on successive projection algorithm；
(6) the prediction yeast activity of the Quantitative Analysis Model described in characteristic spectrum step (4) of nucleic acid, protein and the lipid that step (5) is obtained；
(7) predicting the outcome of step (6) is shown over the display。
Above-described embodiment is this utility model preferably embodiment; but embodiment of the present utility model is also not restricted by the embodiments; other any without departing from the change made under spirit of the present utility model and principle, modification, replacement, combination, simplification; all should be the substitute mode of equivalence, be included within protection domain of the present utility model。
Claims (4)
1. based on the brewing fermentation liquid yeast activity on-line measuring device of Raman spectrum, it is characterised in that include pump, sample cell, three-way valve, flow cell, laser instrument, optical fiber, Raman probe, reflecting mirror, spectrogrph, computer；
The arrival end of described pump is connected with the sample tap of fermentation tank, and delivery side of pump end is connected with the injection port of sample cell；The outlet of described sample cell is connected with the import of three-way valve, and two outlets of described three-way valve are connected with fermentation tank and flow cell respectively；
Described laser instrument is connected to Raman probe by optical fiber, and the head of described Raman probe is connected with the first surface of sample cell, and described reflecting mirror is located on the second surface of described sample cell, and described first surface and second surface are parallel to each other；Described spectrogrph is connected to computer by communication interface；Described sample cell is made of clear material；
Described Raman probe is connected with spectrogrph by optical fiber。
2. the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum according to claim 1, it is characterised in that also include display；Described computer is connected to display by COM1。
3. the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum according to claim 1, it is characterised in that described laser instrument is visible laser or infrared light laser instrument。
4. the brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum according to claim 1, it is characterised in that described Raman probe is the Raman probe of focus adjustable。
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548138A (en) * | 2016-01-13 | 2016-05-04 | 华南理工大学 | On-line wine making fermentation liquid yeast activity detection device and method based on Raman spectrum |
| CN113008784A (en) * | 2019-12-19 | 2021-06-22 | 山东省科学院生物研究所 | Optical fiber device and method for detecting reducing sugar |
| IT202000012610A1 (en) * | 2020-05-27 | 2021-11-27 | Univ Degli Studi Di Firenze | DEVICE AND PROCEDURE FOR THE ANALYSIS OF BODY FLUID SAMPLES |
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2016
- 2016-01-13 CN CN201620031217.3U patent/CN205333518U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548138A (en) * | 2016-01-13 | 2016-05-04 | 华南理工大学 | On-line wine making fermentation liquid yeast activity detection device and method based on Raman spectrum |
| CN105548138B (en) * | 2016-01-13 | 2018-04-13 | 华南理工大学 | The detection method of brewing fermentation liquid yeast activity on-line measuring device based on Raman spectrum |
| CN113008784A (en) * | 2019-12-19 | 2021-06-22 | 山东省科学院生物研究所 | Optical fiber device and method for detecting reducing sugar |
| IT202000012610A1 (en) * | 2020-05-27 | 2021-11-27 | Univ Degli Studi Di Firenze | DEVICE AND PROCEDURE FOR THE ANALYSIS OF BODY FLUID SAMPLES |
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