CN106872462A - The developer and application method of quick detection whiteness flour wheat seed resource high - Google Patents
The developer and application method of quick detection whiteness flour wheat seed resource high Download PDFInfo
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- CN106872462A CN106872462A CN201710216498.9A CN201710216498A CN106872462A CN 106872462 A CN106872462 A CN 106872462A CN 201710216498 A CN201710216498 A CN 201710216498A CN 106872462 A CN106872462 A CN 106872462A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
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Abstract
The invention discloses the developer and application method of quick detection whiteness flour wheat seed resource high, belong to wheat flour whiteness detection technique field.The present invention adjusts phenol solution concentration on the basis of using phenol reagent, adds the solution of certain density cupric sulfate pentahydrate, two kinds of various concentrations to be mixed into developer immersion seed, by seed coat staining reaction, identifies whiteness flour Wheat Germplasm Resources high.The complex large-sized instrument of task is not needed, detection Flour whiteness has been moved to field from laboratory simultaneously, seed can be whenever and wherever possible gathered in big field using the present invention, being then placed in small beaker directly carries out staining reaction and detect whiteness flour Wheat Germplasm Resources high.
Description
Technical field
The invention belongs to whiteness flour wheat seed detection technique field high, a kind of quick detection whiteness flour high is refered in particular to small
The developer of wheat germ plasm resource
Background technology
Whiteness is one of flower characters primary sensory's index, and China consumption of resident person has higher requirements to Flour whiteness, city
Flour whiteness requirement more than 80 on field, more in whiteness wheat breed breeding process high is also that the germplasm for screen whiteness high is provided
Source, i.e., Flour whiteness is more than 80.It is whiteness instrument measure, near-infrared reflection to determine the conventional method of wheat flour whiteness at present
Instrument, the detection of visual near-infrared reflection instrument, also have using phenol immersion the wheat seed 15min, Ran Houqu that mass concentration is 1.0%
Go out to be placed on and be covered with the culture dish that mass concentration is the filter paper that 1% phenol wet is crossed, covered on culture dish lid and equally use phenol
The filter paper for moistening, puts in 37 DEG C of incubator, 25min is dyeed, according to coloration result, indirect detection Flour whiteness.
Although the method for existing detection Flour whiteness can detect Flour whiteness, there are many weak points, whiteness
Instrument determine Flour whiteness shortcoming be：Sample preparation is cumbersome, and testing expenses are high, and the time is more long, and required instrument is more, and these instrument
Device is all expensive, while detecting that whiteness needs the amount of seed larger.Near-infrared reflection instrument and visual near-infrared reflection instrument are determined
Flour whiteness must determine the chemical score that test substance is constituted to come by chemical analysis method, then by Chemical Measurement
Software sets up model, and the near infrared spectrum of unknown sample is input into built model could be predicted to the property of sample.Together
When in order to improve the applicability of model, constantly model is safeguarded and is updated, expend substantial amounts of manpower, energy, financial resources；Profit
The method for coordinating drying box INDIRECTLY IDENTIFIED Flour whiteness with phenol reagent, step is relatively complicated, and the time is slightly long, while needing price
Expensive drying box.
The present invention adjusts phenol solution concentration on the basis of using phenol reagent, adds certain density five water sulfuric acid
Copper, the solution of two kinds of various concentrations is mixed into developer immersion seed, by seed coat staining reaction, identifies whiteness flour high
Wheat Germplasm Resources.The complex large-sized instrument of task is not needed, while detection Flour whiteness has been moved to field from laboratory,
Seed can be whenever and wherever possible gathered in big field using the present invention, then be placed in small beaker that directly to carry out staining reaction detection high
Whiteness flour Wheat Germplasm Resources.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of quick inspection of one kind saved detection time, reduce testing cost
The developer of whiteness flour Wheat Germplasm Resources high is surveyed, can faster detect that whiteness flour high is small in laboratory and big field
Wheat germ plasm resource.Invention reagent composition is as follows：
Reagent 1, configuration quality concentration is 3.0% phenol solution：Weigh during 3g phenol is dissolved into 100ml water and be
3.0% phenol solution；
Reagent 2：The cupric sulfate pentahydrate solution of 1mol/L is configured, CuSO is weighed4(H2O)525g is dissolved into 100ml water i.e.
It is the cupric sulfate pentahydrate solution of 1mol/L；
Wherein reagent 1 and the volume ratio of reagent 2 is 20：(0.15-0.2).
A kind of method using above-mentioned developer quick detection whiteness flour Wheat Germplasm Resources high, enters as steps described below
OK：
(1) reagent is configured：
Reagent 1, configuration quality concentration is 3.0% phenol solution：Weigh during 3g phenol is dissolved into 100ml water and be
3.0% phenol solution；
Reagent 2：The cupric sulfate pentahydrate solution of 1mol/L is configured, CuSO is weighed4(H2O)525g is dissolved into 100ml water i.e.
It is the cupric sulfate pentahydrate solution of 1mol/L.
(2) number takes germ plasm resource：
Number takes every part Wheat Germplasm Resources 50-100, during seed is put into carton or small beaker, is repeated 3 times.
(3) developer is configured：
3.0% reagent 1 (1.0% phenol solution) 20ml is first added toward culture dish, (the 1mol/L of reagent 2 is subsequently adding
CuSO4(H2O)5Solution) 0.15-0.2ml mixings, as developer.
(4) chromogenic reaction.In seed to be detected immersion developer, (20 DEG C) are soaked seed 25 minutes under normal temperature.
(5) coloration result is observed.
Immersion starts to detect seed coat color for 25 minutes afterwards.
(6) colour developing identification.
Standard of perfection：(1) compared with untreated wheat seed resource, the achromophil seed of Wheat Species skin is whiteness value
(elegant demeanour instruments and meters Co., Ltd, digital display whiteness instrument, model are pleased 80 with Shanghai:SBDY-1 carries out result verification, and the result is shown in
Table 1) more than wheat seed resource, such seed resource for whiteness wheat seed resource (2) high dye it is light brown or brown
It is the wheat seed resource of low whiteness wheat seed resource, i.e. whiteness value below 79.
The present invention is convenient and swift, time saving quick.Do not need instrument and equipment, need not fix experiment place, required article compared with
Few, minute only needs 25min.The accuracy rate of the above method is 96%.
Advantages of the present invention：
1. technical method is simple, convenient and swift, without instrument and equipment, it is only necessary to medicine, culture dish.
2. detect quick, required time is short, and whole process is only needed 25 minutes.
3. it is simple to operate, can be detected in field.
4. cost needed for detecting is cheaper.
Brief description of the drawings
Fig. 1 is the chromogenic reaction picture using developer of the present invention treatment Wheat Germplasm Resources Z1-Z24；
Fig. 2 is the chromogenic reaction picture using developer of the present invention treatment Wheat Germplasm Resources Z25-Z48；
Fig. 3 is the chromogenic reaction picture using developer of the present invention treatment Wheat Germplasm Resources Z49-Z72；
Fig. 4 is the chromogenic reaction picture using developer of the present invention treatment wheat seed resource Z73-Z96；
Fig. 5 is the chromogenic reaction picture using developer of the present invention treatment Wheat Germplasm Resources Z97-Z120；
Fig. 6 is the chromogenic reaction picture using developer of the present invention treatment Wheat Germplasm Resources Z121-Z128.
Specific embodiment
Particular content of the invention is specifically described below in conjunction with example, while with application for a patent for invention (application number:
201610526685.2) superiority of the invention is illustrated to contrast：
1. with 20 parts of wheat breed (strain) resources of random selection in seed resource storehouse as material to be tested
(1) application for a patent for invention (application number:CN201610526685.2 the method for) detecting whiteness flour seed resource high
Step is：
1. reagent is configured：1% phenol solution (1g phenol is dissolved in 100ml water).
2. number takes Wheat Germplasm Resources 100, and 15min is soaked with 1.0% phenol.
3. the seed for soaking takes out to be placed on and is covered with the culture dish of the filter paper that 1% phenol wet is crossed (ventral groove is downward), trains
The same filter paper crossed with phenol wet of covering on ware lid is supported, culture dish is covered.
4. and then culture dish is put in 37 DEG C of incubator, dyes 25min, observe coloration result.
Standard of perfection：(1) achromophil seed is whiteness high (whiteness value is more than 80) wheat breed seed.
(2) it is (whiteness value is below 79) low whiteness wheat breed seed to dye light brown or brown.
(2) method of the present invention：A kind of developer of quick detection whiteness flour wheat seed resource high detects whiteness high
Under the method and step of flour seed resource enters：
1. reagent one is configured：3.0% phenol solution, reagent two：Configure the CuSO of 1mol/L4(H2O)5Solution；Then first
The 20ml of 3.0% reagent one is added toward culture dish, the 0.15ml of reagent two is added, is mixed and is developer to be measured.
2. number takes Wheat Germplasm Resources：Number takes every part of Wheat Germplasm Resources 100, seed is put into developer and is submerged.
3. soak 25 minutes at normal temperatures.Observation coloration result.
Standard of perfection：(1) compared with untreated wheat seed resource, the achromophil seed of Wheat Species skin is whiteness value
(elegant demeanour instruments and meters Co., Ltd, digital display whiteness instrument, model are pleased 80 with Shanghai:SBDY-1 has carried out result verification, and accuracy rate exists
96% the result is shown in Table the wheat seed resource more than 1), and such seed resource is dyed for whiteness wheat seed resource (2) high
Light brown or brown is the wheat seed resource of low whiteness wheat seed resource, i.e. whiteness value below 79.
2. the advantage implemented using the present invention
The present invention reduces the ring reacted in being put into culture dish and incubator after immersion wheat resource 15 minutes and immersion
Section, the link of reduction equally reduces the use of filter paper, culture dish, incubator or drying box.Compared to existing technology, detection time contracting
It is as short as 25 minutes, saves a large amount of valuable times of scientific research personnel.The use of incubator, filter paper, culture dish is reduced, it is also just relative
The recruitment of whiteness detection and the input of various goods and materials are reduced, so as to reduce testing cost；Because detection only needs to exist in advance
After laboratory has configured developer, the experiment of detection Flour whiteness can be moved to field from laboratory, planted harvesting
In the season of matter resource, seed can be whenever and wherever possible gathered in big field, then only need to be put into the small beaker for filling developer
Staining reaction directly is carried out, whiteness flour Wheat Germplasm Resources high can be just detected within 25 minutes.It is time saving and energy saving using the method, side
Just it is efficient.
Specifically detect that the method for whiteness flour Wheat Germplasm Resources high is as follows using the present invention：
Material：128 parts of Wheat Germplasm Resources of random selection
Medicine：Phenol, CuSO4(H2O)5
Utensil：Culture dish, filter paper
Method and step：
First, reagent is configured：
Reagent 1, the phenol solution of configuration 3.0%：Weigh as 3.0% phenol during 3 grams of phenol are dissolved into 100ml water molten
Liquid；
Reagent 2：Configure the CuSO of 1mol/L4(H2O)5Solution：Weigh CuSO4(H2O)525 grams are dissolved into 100ml water i.e.
It is the cupric sulfate pentahydrate solution of 1mol/L.
2nd, number takes Wheat Germplasm Resources：Number takes every part of Wheat Germplasm Resources 100, and seed is put into culture dish, repeats
Three times.
3rd, developer is configured：First toward adding 3.0% reagent 1 (1.0% phenol solution) 20ml, Ran Houjia in culture dish
Enter (the 1mol/L CuSO of reagent 24(H2O)5Solution) 0.15ml.Soak 25 minutes at normal temperatures.
4th, coloration result is observed.Coloration result see the table below 1；The accuracy rate of the method is 96%.
Result verification
(elegant demeanour instruments and meters Co., Ltd, model are pleased with digital display whiteness instrument in Shanghai:SBDY-1 Wheat Species) are processed to developer
Matter resource results are verified that digital display whiteness instrument determines Z1-Z128 germ plasm resource Flour whiteness, verifies this developer measurement result
Reliability.
Whiteness instrument verification method step is as follows：
Take above-mentioned 128 parts of wheat seeds
(1) wheat seed resource each 300g of net seed is weighed, moisture is determined after drying 60min at 130-133 DEG C.
(2) seed is carried out into wet process, its water content is reached 15.0%.
(3) it is milled, calculates flour extraction.Flour is ground to be placed 1 week in laboratory.
(4) measuring brightness：Weigh flour digital display whiteness instrument and determine Z1-Z128 wheat seed Flour whiteness, and record white
Angle value, measuring brightness the results are shown in Table 1.
Table 1
3. the method application in practice.
(1) whiteness Wheat Germplasm Resources high are screened.The method can be used for wheat breeding scientific research institution of universities and kind
The Wheat Germplasm Resources of the seed selections such as sub- research and development department whiteness wheat breed high are quickly screened.
(2) flour enterprises quickly screen whiteness wheat breed high.Flour mill is in purchase wheat breed and Flour production mistake
The quick whiteness for understanding purchase wheat in journey, to provide reference with powder.
(3) wheat breed improvement.Germ plasm resource with whiteness as criteria for classification is poor, is whiteness genetic improvement in practice
Difficulty；The Wheat Germplasm Resources of whiteness high can be screened with quick detection in this approach, excavates that Flour whiteness is high, that quality is excellent is small
Wheat seeds matter, by seed selection whiteness new variety of wheat high, can meet the requirement of breeding and people to flour-made food whiteness high.And
Can be few in raw material, in the case that the used time is short, the wheat flour of whiteness high is quickly measured, for the detection of this index of whiteness high provides pole
It is big convenient.
(4) application during Wheat Cultivars.During the Single-plant selection in Wheat Cultivars key epoch, often
Grain weight is less, can just identify the wheat individual plant or strain of whiteness high with minimal amount of seed with the method.
4. the developer determines the advantage of whiteness
1. it is convenient and swift, it is time saving quick.Medicine, culture dish are only needed to, tweezers minute only needs 25min.
2. operating method is simple and easy to apply.The method is easy to grasp for basic unit's scientific research breeding and little company breeder, while
Expense is relatively low.It is easy to be promoted the use of in basic unit.
3. it is with low cost.Phenol price is 20 yuan of 1 bottle of 500g, can at least be used 500 times.Each cost is 0.04 yuan.
Instrument article needed for determining is tested simultaneously few so that determine whiteness cost of consumption lower.
Claims (3)
1. the developer of a kind of quick detection whiteness flour Wheat Germplasm Resources high, it is characterised in that composition is as follows：
Reagent 1, configuration quality concentration is 3.0% phenol solution：Weigh during 3g phenol is dissolved into 100ml water and be 3.0% phenol
Solution；
Reagent 2：The cupric sulfate pentahydrate solution of 1mol/L is configured, CuSO is weighed4(H2O)525g is in being dissolved into 100ml water
The cupric sulfate pentahydrate solution of 1mol/L；
Wherein reagent 1 and the volume ratio of reagent 2 is 20：（0.15-0.2）.
2. small using a kind of above-mentioned developer quick detection whiteness flour high of quick detection whiteness flour Wheat Germplasm Resources high
The method of wheat germ plasm resource, it is characterised in that carry out as steps described below：
（1）Configuration reagent：
Reagent 1, configuration quality concentration is 3.0% phenol solution：Weigh during 3g phenol is dissolved into 100ml water and be 3.0% phenol
Solution；
Reagent 2：The cupric sulfate pentahydrate solution of 1mol/L is configured, CuSO is weighed4(H2O)525g is in being dissolved into 100ml water
The cupric sulfate pentahydrate solution of 1mol/L；
（2）Number takes germ plasm resource：
Number takes every part Wheat Germplasm Resources 50-100, during seed is put into carton or small beaker, is repeated 3 times；
（3）Configuration developer：
3.0% reagent 1 is first added toward culture dish（1.0% phenol solution）20ml, is subsequently adding reagent 2（1mol/L CuSO4
(H2O)5Solution）0.15-0.2ml is mixed, as developer；
（4）Chromogenic reaction；Seed to be detected is immersed in developer, under normal temperature（20℃）Immersion seed 25 minutes；
（5）Observation coloration result；
Immersion starts to detect seed coat color for 25 minutes afterwards；
（6）Colour developing identification.
3. the method for a kind of quick detection according to claim 2 whiteness flour Wheat Germplasm Resources high, it is characterised in that
Standard of perfection is：（1）Compared with untreated wheat seed resource, the achromophil seed of Wheat Species skin is whiteness value 80
（Elegant demeanour instruments and meters Co., Ltd, digital display whiteness instrument, model are pleased with Shanghai:SBDY-1 carries out result verification, and the result is shown in Table 1）
Wheat seed resource above, such seed resource is whiteness wheat seed resource high（2）Dye light brown or brown for low
The wheat seed resource of whiteness wheat seed resource, i.e. whiteness value below 79.
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| CN201710216498.9A CN106872462A (en) | 2017-04-05 | 2017-04-05 | The developer and application method of quick detection whiteness flour wheat seed resource high |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110082348A (en) * | 2019-06-21 | 2019-08-02 | 福建农林大学 | A kind of method of rice Zn content rapid dyeing identification |
| CN110095329A (en) * | 2019-06-06 | 2019-08-06 | 山东农业大学 | A kind of colouring method of quick measurement wheat seed purity |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198406A (en) * | 2016-07-05 | 2016-12-07 | 河南科技学院 | A kind of method of quick detection high whiteness flour wheat seed |
-
2017
- 2017-04-05 CN CN201710216498.9A patent/CN106872462A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198406A (en) * | 2016-07-05 | 2016-12-07 | 河南科技学院 | A kind of method of quick detection high whiteness flour wheat seed |
Non-Patent Citations (2)
| Title |
|---|
| BANERJEE , S.K.: "Modified phenol test for the varietal identification of wheat seed", 《SEED SCIENCE AND TECHNOLOGY》 * |
| 尹燕枰，董学会: "《种子学实验技术》", 28 February 2008, 中国农业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110095329A (en) * | 2019-06-06 | 2019-08-06 | 山东农业大学 | A kind of colouring method of quick measurement wheat seed purity |
| CN110082348A (en) * | 2019-06-21 | 2019-08-02 | 福建农林大学 | A kind of method of rice Zn content rapid dyeing identification |
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Application publication date: 20170620 |