CN101893575A - Method for rapidly testing nitrite in fresh milk - Google Patents
Method for rapidly testing nitrite in fresh milk Download PDFInfo
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- CN101893575A CN101893575A CN 201010141449 CN201010141449A CN101893575A CN 101893575 A CN101893575 A CN 101893575A CN 201010141449 CN201010141449 CN 201010141449 CN 201010141449 A CN201010141449 A CN 201010141449A CN 101893575 A CN101893575 A CN 101893575A
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- nitrite
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Abstract
The invention provides a method for rapidly testing nitrite in fresh milk. The method comprises the following steps: adding 3-6ml of milk sample in a colorimetric tube, then adding 1-2ml of 20% tartaric acid solution and 1-2ml of 0.5% sulfanilic acid solution in turn, shaking evenly, adding 1-2ml of 0.3% N(1-naphty1)-ethylenediamine dihydrochloride solution, wherein the lower dosage of 20% tartaric acid solution is 1ml, the lower dosage of 0.5% sulfanilic acid solution is 1ml, and the lower dosage of 0.3% N(1-naphty1)-ethylenediamine dihydrochloride solution is 1ml; and observing after fully shaking, wherein if different shades of pink appears in the colorimetric tube, the sample is proved to contain nitrite. The size of the selected colorimetric tube with a stopper is 15-20ml. The lower limit detection value of the method is 0.1mu g/ml; and the effects of the method are that the dosage is small, the preservation time is long and the detection sensitivity is high. The reagent kit prepared by the method is more convenient to use.
Description
Technical field
The present invention relates to the quality testing of fresh milk, especially judge and contain nitrite composition, for ensureing its food security, it is more convenient that the method is made the checkbox use.
Background technology
Along with growth in the living standard, milk has been can not obtain scarce important component part during people live.Milk is rich in protein, fat, carbohydrates, trace element and vitamin etc., and edible milk can be built up health, and improves intelligence, therefore is subjected to liking of consumers in general deeply.But in order to reap staggering profits, the lawless person adds nitrite in fresh milk be common occurrence.Nitrite directly acts on people's blood vessel, can cause peripheral vasodilation, and can form methemoglobin with haemoglobin in blood, occur dizzy, feel sick, vomiting even clinical symptoms such as blood pressure drops or shock.In order to ensure the healthy of consumer, must carry out strict detection to mixing of Ruzhong nitrite, determine the price with matter for the cow's milk purchasing station simultaneously objective basis is provided.Have literature search to disclose: " method for quick of fresh milk adulterant " patent (CN87103531A) can detect kinds more than 20 such as sulfate in the milk, nitrite, washing powder, advertisement are white, zinc paste, sucrose, urine, coating and mingle material.Design of the present invention is different with it is that reality detection method is efficiently adopted more in the detection of nitrite composition, and then constantly promotes the level of scientific management and the technological means in milk industry market.
Summary of the invention
The objective of the invention is to: contain the method for nitrite in the fast detecting fresh milk, operation at normal temperatures is not only easy but also accurate, and particularly the use of generate a reagent checkbox will be more quick.
The object of the present invention is achieved like this: the method for nitrite in a kind of fast detecting fresh milk; get color comparison tube and add milk sample 3-6ml; add 1-2ml 20% tartaric acid solution then successively; the sulfanilic acid solution of 1-2ml 0.5%, shake up the hydrochloride naphthodiamide that the back adds 1-2ml 0.3%; The dosage of its lower limit is formulated as the tartaric acid solution of 1ml 20%, the sulfanilic acid solution of 1ml 0.5%, the hydrochloride naphthodiamide solution of 1ml 0.3%; Shake observation through fully mixing, the pink of different depth occurs, show to be mixed with nitrite in the sample.
The method of nitrite in the described detection fresh milk, the color-comparison tube specification of selecting for use is 15-20ml.
The method of nitrite in the described detection fresh milk, determined detection lower limit are 0.1 μ g/ml, and its effect is that consumption is little, and the holding time is long, measure highly sensitive.
The method of nitrite in the described detection fresh milk, made reagent detects box, and it is more convenient to use.
The method of nitrite in the described detection fresh milk, the raw material of selecting for use is the commercially available prod.
The present invention conceives mechanism: in acid solution, and NO2
-After sulfanilic acid diazotising, be coupled to peach azo-compound with hydrochloride naphthodiamide again.Therefore the interpolation of reagent is in proper order: first tartarize solution, add sulfanilic acid solution again, and add hydrochloride naphthodiamide after shaking up.
This reagent test method is compared in the effect dative of the present invention, fills a prescription to be tartrate (C
4H
8O
6) 89g, anhydrous sulfanilic acid (C
6H
4(NH
2) (SO
3H)) 10g, alpha-naphthylamine 1g through careful porphyrize, fully mix the Griess reagent that uses, and when consumption was 0.3g, best results, the lower limit of detection were 0.133 μ g/ml, apparently higher than the standard of this detection method.
The characteristics of detection method of the present invention are preferred tartaric acid solution, the best percentage composition and the consumption of sulfanilic acid solution, hydrochloride naphthodiamide; The lower control limit value promptly reaches the design effect of fast detecting, sees Table 1.
Table 1 nitrite detects determining of lower limit
As can be seen from Table 1: be limited to 0.1 μ g/ml under the detection of this reaction system.
The method that contains nitrite in the fast detecting fresh milk of the present invention not only for the cow's milk purchase provides the foundation of determining the price with matter, simultaneously to the science law enforcement, and detects fast, shows technical progress.
Embodiment
Embodiment
Get color comparison tube and add milk sample 5ml, add 1.0ml 20% tartaric acid solution then successively, the sulfanilic acid solution of 1.5ml 0.5% shakes up the hydrochloride naphthodiamide that the back adds 1.3ml 0.3%; The tartaric acid solution that is formulated as 1ml 20% of its lower limit dosage, the sulfanilic acid solution of 1ml 0.5%, the hydrochloride naphthodiamide solution of 1ml 0.3%; Shake observation through fully mixing, the pink of different depth occurs, show to be mixed with nitrite in the sample.
Wherein:
1, determining of tartaric acid solution consumption sees Table 2.
Table 2
Color developing effect shows in the above-mentioned table: the tartaric acid solution consumption of determining 1ml 20% is good.
2, determining of sulfanilic acid consumption sees Table
23.
Table 3
Color developing effect shows in the above-mentioned table: determine that 0.5% sulfanilic acid solution usage is 1ml.
3, determining of hydrochloride naphthodiamide consumption sees Table 4.
Table 4
The color developing effect of above-mentioned table shows: determine that 0.3% hydrochloride naphthodiamide consumption is 1ml.
The present invention adopts the screening of the consumption of this prescription, and purpose makes blank and mingle the sample color distinction more remarkable, and is easy to most judge, and can save reagent etc.
Claims (5)
1. the method for nitrite in the fast detecting fresh milk, it is characterized in that: get color comparison tube and add milk sample 3-6ml, add 1-2ml 20% tartaric acid solution then successively, the sulfanilic acid solution of 1-2ml 0.5% shakes up the hydrochloride naphthodiamide that the back adds 1-2ml 0.3%; The dosage of its lower limit is formulated as the tartaric acid solution of 1ml 20%, the sulfanilic acid solution of 1ml 0.5%, the hydrochloride naphthodiamide solution of 1ml 0.3%; Shake observation through fully mixing, the pink of different depth occurs, show to be mixed with nitrite in the sample.
2. according to the method for nitrite in the described detection fresh milk of claim 1, it is characterized in that: the color-comparison tube specification of selecting for use is 15-20ml.
3. according to the method for nitrite in the described detection fresh milk of claim 1, it is characterized in that: determined detection lower limit is 0.1 μ g/ml, and its effect is that consumption is little, and the holding time is long, measures highly sensitive.
4. according to the method for nitrite in the described detection fresh milk of claim 1, it is characterized in that: method therefor is made into reagent and detects box, and it is more convenient to use.
5. according to the method for nitrite in the described detection fresh milk of claim 1, it is characterized in that: the raw material of selecting for use is the commercially available prod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010141449 CN101893575A (en) | 2010-04-08 | 2010-04-08 | Method for rapidly testing nitrite in fresh milk |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010141449 CN101893575A (en) | 2010-04-08 | 2010-04-08 | Method for rapidly testing nitrite in fresh milk |
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| CN101893575A true CN101893575A (en) | 2010-11-24 |
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| CN 201010141449 Pending CN101893575A (en) | 2010-04-08 | 2010-04-08 | Method for rapidly testing nitrite in fresh milk |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018232A (en) * | 2011-09-20 | 2013-04-03 | 内蒙古蒙牛乳业（集团）股份有限公司 | Detection method for sulfite in milk |
| CN103487434A (en) * | 2013-10-14 | 2014-01-01 | 无锡艾科瑞思产品设计与研究有限公司 | Method for detecting whether nitrite content in smoked sauce food exceeds standard |
| CN103487386A (en) * | 2013-10-14 | 2014-01-01 | 无锡艾科瑞思产品设计与研究有限公司 | Method for detecting nitrate content in liquid food |
| CN103529031A (en) * | 2013-11-01 | 2014-01-22 | 内蒙古农业大学 | Semi-solid-type nitrite rapid detection tube and method for preparing same |
| CN104280383A (en) * | 2013-07-02 | 2015-01-14 | 云南健牛生物科技有限公司 | Rapid detection method for nitrites in blood and urine |
| CN104407097A (en) * | 2014-11-28 | 2015-03-11 | 苏州东辰林达检测技术有限公司 | Detection reagent and method of nitrite |
| CN104458737A (en) * | 2014-12-29 | 2015-03-25 | 贝因美婴童食品股份有限公司 | Semi-quantitative nitrite rapid detecting composition and application thereof |
| CN105628634A (en) * | 2016-03-02 | 2016-06-01 | 山东安博仪器股份有限公司 | Reagent for detecting nitrite in food and nitrite detection method |
| CN105651768A (en) * | 2015-12-29 | 2016-06-08 | 内蒙古蒙牛乳业（集团）股份有限公司 | Color developing agent composition and application thereof as well as method for detecting nitritein sample |
| CN106596540A (en) * | 2016-12-20 | 2017-04-26 | 江苏食品药品职业技术学院 | Chromogenic agent and watered cow milk determination method |
| CN109827921A (en) * | 2019-03-12 | 2019-05-31 | 闫宏涛 | A kind of NO3-N and NO2-N assay analoids |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101290323A (en) * | 2008-05-20 | 2008-10-22 | 内蒙古蒙牛乳业（集团）股份有限公司 | Cow milk and milk powder nitrite qualitative checking method and reagent kit |
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| CN101290323A (en) * | 2008-05-20 | 2008-10-22 | 内蒙古蒙牛乳业（集团）股份有限公司 | Cow milk and milk powder nitrite qualitative checking method and reagent kit |
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| 《GB 5009.33-85食品中亚硝酸盐与硝酸盐的测定方法》 19850516 卫生部食品卫生监督检验所 食品中亚硝酸盐与硝酸盐的测定方法 1-4 1-5 , 1 * |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018232A (en) * | 2011-09-20 | 2013-04-03 | 内蒙古蒙牛乳业（集团）股份有限公司 | Detection method for sulfite in milk |
| CN104280383A (en) * | 2013-07-02 | 2015-01-14 | 云南健牛生物科技有限公司 | Rapid detection method for nitrites in blood and urine |
| CN103487434A (en) * | 2013-10-14 | 2014-01-01 | 无锡艾科瑞思产品设计与研究有限公司 | Method for detecting whether nitrite content in smoked sauce food exceeds standard |
| CN103487386A (en) * | 2013-10-14 | 2014-01-01 | 无锡艾科瑞思产品设计与研究有限公司 | Method for detecting nitrate content in liquid food |
| CN103529031A (en) * | 2013-11-01 | 2014-01-22 | 内蒙古农业大学 | Semi-solid-type nitrite rapid detection tube and method for preparing same |
| CN104407097A (en) * | 2014-11-28 | 2015-03-11 | 苏州东辰林达检测技术有限公司 | Detection reagent and method of nitrite |
| CN104458737A (en) * | 2014-12-29 | 2015-03-25 | 贝因美婴童食品股份有限公司 | Semi-quantitative nitrite rapid detecting composition and application thereof |
| CN105651768A (en) * | 2015-12-29 | 2016-06-08 | 内蒙古蒙牛乳业（集团）股份有限公司 | Color developing agent composition and application thereof as well as method for detecting nitritein sample |
| CN105628634A (en) * | 2016-03-02 | 2016-06-01 | 山东安博仪器股份有限公司 | Reagent for detecting nitrite in food and nitrite detection method |
| CN106596540A (en) * | 2016-12-20 | 2017-04-26 | 江苏食品药品职业技术学院 | Chromogenic agent and watered cow milk determination method |
| CN109827921A (en) * | 2019-03-12 | 2019-05-31 | 闫宏涛 | A kind of NO3-N and NO2-N assay analoids |
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Open date: 20101124 |