CN103487386A - Method for detecting nitrate content in liquid food - Google Patents
Method for detecting nitrate content in liquid food Download PDFInfo
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- CN103487386A CN103487386A CN201310478133.5A CN201310478133A CN103487386A CN 103487386 A CN103487386 A CN 103487386A CN 201310478133 A CN201310478133 A CN 201310478133A CN 103487386 A CN103487386 A CN 103487386A
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Abstract
The invention discloses a method for detecting the nitrate content in liquid food, belongs to the field of food security detection, and in particular relates to a method for detecting the nitrate content in the liquid food. The method comprises the following steps of firstly, establishing a nitrate content detection curve, secondly, preparing a liquid food detection solution, and acquiring the nitrate content in the liquid food to be detected according to a corresponding relationship of a red coverage gray scale value of the liquid food detection solution and the nitrate content detection curve. The invention not only provides the method for detecting the nitrate content in the liquid food, but also a method for indirectly measuring the nitrate content in a machine view mode, and the method is convenient to operate and convenient to popularize, and has the advantage that the measurement speed is high.
Description
Technical field
A kind of detection method of food liquid nitrite belongs to the food safety detection field, is specifically related to a kind of detection method of food liquid nitrite.
Background technology
Nitrite, the general name of a class mineral compound.Mainly refer to sodium nitrite, sodium nitrite is white to pale yellow powder or graininess, distinguishes the flavor of micro-salty, soluble in water.Outward appearance and flavour are all similar to salt, and in industry, widely use in building industry, in meat product, also allow as the colour former use of limiting the quantity of.
Yet, some illegal retailers, for the purpose of getting a profit by fair means or foul, add the nitrite goods in food, cause food-safety problem.Nitrite directly acts on people's blood vessel, can cause peripheral vasodilation, and can form methemoglobin with haemoglobin in blood, and dizziness occurring, feel sick, vomit the even clinical symptoms such as blood pressure drops or shock, the nitrite of eating 0.3～0.5 gram can cause poisoning even dead.More seriously, nitrite is typical carcinogenic substance simultaneously.Therefore, healthy in order to ensure the consumer, be necessary smoked sauce content of nitrite in foods is carried out to strict detection and control.
Traditional nitrite detection method step complexity, and length consuming time, and patent of invention " a kind of method of Raw Milk nitrite ", application number: 201010141449.1, content of nitrite in can Raw Milk, this method, can be used for other food liquid of direct-detection.Yet this method can only detect whether contain the nitrite goods, and can not determine its accurate content.
Summary of the invention
In order to address the above problem, the present invention has designed a kind of detection method of food liquid nitrite, a kind of new content of nitrite assay method not only is provided, and employing machine vision means, indirectly measure the method for content of nitrite, easy to operate, be convenient to popularize, therefore also there is the fast advantage of measuring speed.
The object of the present invention is achieved like this:
A kind of detection method of food liquid nitrite comprises the following steps:
Step a, set up content of nitrite and measure curve
Get respectively sodium nitrite 1mg, 2mg, 3mg, 4mg and 5mg, be dissolved in the tartaric acid solution that 100ml concentration is 20%, the mixed liquor of the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 100ml concentration is 0.5% and 100ml concentration are 0.3%, pouring distilled water to solution quality into respectively in 5 mixed liquors is 1kg, after abundant mixed shaking, in solution, the pink of different gray scales will appear, with colored CCD or CMOS to the solution imaging, get red figure layer, record its gray-scale value, and draw gray-scale value-sodium nitrite figure-of-merit curve;
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, with the 100ml concentration tartaric acid solution that is 20%, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 100ml concentration is 0.5% and 100ml concentration are 0.3% mixes, pouring distilled water to solution quality into is 1kg again, after abundant mixed shaking, to the solution imaging, get red figure layer with colored CCD or CMOS, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
The gray-scale value obtained according to step b, the gray-scale value that contrast step a obtains-sodium nitrite figure-of-merit curve, calculate the contained content of sodium nitrite of 0.5kg food liquid to be measured.
The detection method of above-mentioned a kind of food liquid nitrite also comprises steps d: the content of sodium nitrite that step c is obtained * 2 obtain the contained content of sodium nitrite of 1kg food liquid to be measured.
The detection method of above-mentioned a kind of food liquid nitrite, the gray-scale value in described step a-sodium nitrite figure-of-merit curve, adjacent 2 connect for straight line.
The detection method of above-mentioned a kind of food liquid nitrite, the gray-scale value in described step a-sodium nitrite figure-of-merit curve, be 5 Function Fitting curves.
The detection method of above-mentioned a kind of food liquid nitrite, can also adopt the tartaric acid solution that 50ml concentration is 40% in step a and step b, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 50ml concentration is 1% and 50ml concentration are 0.6%.
The detection method of above-mentioned a kind of food liquid nitrite, can also adopt the tartaric acid solution that 200ml concentration is 10% in step a and step b, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 200ml concentration is 0.25% and 200ml concentration are 0.15%.
The tartaric acid solution adopted in the tartaric acid solution adopted in step b, sulfanilic acid solution and hydrochloride naphthodiamide solution and step a, sulfanilic acid solution and hydrochloride naphthodiamide solution phase are same.
The detection method of above-mentioned a kind of food liquid nitrite, after step a carried out once, the method only includes:
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, with the 100ml concentration tartaric acid solution that is 20%, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 100ml concentration is 0.5% and 100ml concentration are 0.3% mixes, pouring distilled water to solution quality into is 1kg again, after abundant mixed shaking, to the solution imaging, get red figure layer with colored CCD or CMOS, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
The gray-scale value obtained according to step b, the gray-scale value that contrast step a obtains-sodium nitrite figure-of-merit curve, the contained content of sodium nitrite of judgement 0.5kg food liquid to be measured.
The detection method of above-mentioned a kind of food liquid nitrite, described food liquid is milk, wine, beverage, congee, boiling hot, medicine soup.
Because the detection method model content of nitrite of food liquid nitrite of the present invention is measured curve, configure again food liquid and detect solution, detect the corresponding relation of the red figure layer gray-scale value of solution image and content of nitrite mensuration curve by food liquid, obtain food liquid content of nitrite to be measured; The present invention not only provides a kind of new content of nitrite assay method, and adopts the machine vision means, indirectly measures the method for content of nitrite, easy to operate, is convenient to popularize, and therefore also has the fast advantage of measuring speed.
The accompanying drawing explanation
Fig. 1 is the detection method process flow diagram of food liquid nitrite of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the invention is described in further detail.
Specific embodiment one
The detection method of the food liquid nitrite of the present embodiment, as shown in Figure 1, the method comprises the following steps process flow diagram:
Step a, set up content of nitrite and measure curve
Get respectively sodium nitrite 1mg, 2mg, 3mg, 4mg and 5mg, be dissolved in the tartaric acid solution that 100ml concentration is 20%, the mixed liquor of the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 100ml concentration is 0.5% and 100ml concentration are 0.3%, pouring distilled water to solution quality into respectively in 5 mixed liquors is 1kg, after abundant mixed shaking, in solution, the pink of different gray scales will appear, with colored CCD or CMOS to the solution imaging, get red figure layer, record its gray-scale value, and drafting gray-scale value-sodium nitrite figure-of-merit curve, 2 straight lines adjacent in gray-scale value-sodium nitrite figure-of-merit curve connect,
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, with the 100ml concentration tartaric acid solution that is 20%, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 100ml concentration is 0.5% and 100ml concentration are 0.3% mixes, pouring distilled water to solution quality into is 1kg again, after abundant mixed shaking, to the solution imaging, get red figure layer with colored CCD or CMOS, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
The gray-scale value obtained according to step b, the gray-scale value that contrast step a obtains-sodium nitrite figure-of-merit curve, the contained content of sodium nitrite of judgement 0.5kg food liquid to be measured.
For example, when the sodium nitrite quality is respectively 1mg, 2mg, 3mg, 4mg and 5mg, the corresponding gray-scale value of step a is respectively 10,20,32,46,60, the gray-scale value obtained as step b is 15, just between step a gray-scale value 10 and 20, therefore resulting sodium nitrite is between 1mg and 2mg, is 1.5mg, i.e. 1.5mg/ jin.
Specific embodiment two
The present embodiment is different from specific embodiment one, and the present embodiment also comprises steps d: the content of sodium nitrite that step c is obtained * 2 obtain the contained content of sodium nitrite of 1kg food liquid to be measured.Utilize the data of specific embodiment one, finally detecting the content of sodium nitrite obtained is 3mg/kg.
Specific embodiment three
What the present embodiment was different from specific embodiment one is, gray-scale value in the present embodiment step a-sodium nitrite figure-of-merit curve, be 5 Function Fitting curves, although 5 Function Fitting calculated amount are slightly large, be more suitable for the linear poor situation of relation between gray-scale value and sodium nitrite quality.
Specific embodiment four
The present embodiment and different being of embodiment before, can also adopt the tartaric acid solution that 50ml concentration is 40% in step a and step b, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 50ml concentration is 1% and 50ml concentration are 0.6%.The tartaric acid solution adopted in the tartaric acid solution adopted in step b, sulfanilic acid solution and hydrochloride naphthodiamide solution and step a, sulfanilic acid solution and hydrochloride naphthodiamide solution phase are same.
Specific embodiment five
The present embodiment and different being of embodiment before, can also adopt the tartaric acid solution that 200ml concentration is 10% in step a and step b, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 200ml concentration is 0.25% and 200ml concentration are 0.15%.The tartaric acid solution adopted in the tartaric acid solution adopted in step b, sulfanilic acid solution and hydrochloride naphthodiamide solution and step a, sulfanilic acid solution and hydrochloride naphthodiamide solution phase are same.
Specific embodiment six
The detection method of the food liquid nitrite of the present embodiment, after step a carried out once, the method only includes:
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, with the 100ml concentration tartaric acid solution that is 20%, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 100ml concentration is 0.5% and 100ml concentration are 0.3% mixes, pouring distilled water to solution quality into is 1kg again, after abundant mixed shaking, to the solution imaging, get red figure layer with colored CCD or CMOS, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
The gray-scale value obtained according to step b, the gray-scale value that contrast step a obtains-sodium nitrite figure-of-merit curve, the contained content of sodium nitrite of judgement 0.5kg food liquid to be measured.
The detection method of the food liquid nitrite of above embodiment, described food liquid comprises milk, wine, beverage, congee, boiling hot, medicine soup.
The present invention is not limited to above-mentioned preferred forms, and anyone should learn structural change or the method improvement of making under enlightenment of the present invention, and every have identical or close technical scheme with the present invention, within all falling into protection scope of the present invention.
Claims (9)
1. the detection method of a food liquid nitrite is characterized in that: comprise the following steps:
Step a, set up content of nitrite and measure curve
Get respectively sodium nitrite 1mg, 2mg, 3mg, 4mg and 5mg, be dissolved in the tartaric acid solution that 100ml concentration is 20%, the mixed liquor of the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 100ml concentration is 0.5% and 100ml concentration are 0.3%, pouring distilled water to solution quality into respectively in 5 mixed liquors is 1kg, after abundant mixed shaking, in solution, the pink of different gray scales will appear, with colored CCD or CMOS to the solution imaging, get red figure layer, record its gray-scale value, and draw gray-scale value-sodium nitrite figure-of-merit curve;
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, with the 100ml concentration tartaric acid solution that is 20%, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 100ml concentration is 0.5% and 100ml concentration are 0.3% mixes, pouring distilled water to solution quality into is 1kg again, after abundant mixed shaking, to the solution imaging, get red figure layer with colored CCD or CMOS, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
The gray-scale value obtained according to step b, the gray-scale value that contrast step a obtains-sodium nitrite figure-of-merit curve, calculate the contained content of sodium nitrite of 0.5kg food liquid to be measured.
2. the detection method of a kind of food liquid nitrite according to claim 1, it is characterized in that: also comprise steps d: the content of sodium nitrite that step c is obtained * 2 obtain the contained content of sodium nitrite of 1kg food liquid to be measured.
3. the detection method of a kind of food liquid nitrite according to claim 1 is characterized in that: the gray-scale value in described step a-sodium nitrite figure-of-merit curve, adjacent 2 is that straight line connects.
4. the detection method of a kind of food liquid nitrite according to claim 1, it is characterized in that: the gray-scale value in described step a-sodium nitrite figure-of-merit curve is 5 Function Fitting curves.
5. the detection method of a kind of food liquid nitrite according to claim 1, it is characterized in that: can also adopt the tartaric acid solution that 50ml concentration is 40% in step a and step b, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 50ml concentration is 1% and 50ml concentration are 0.6%.
6. the detection method of a kind of food liquid nitrite according to claim 1, it is characterized in that: can also adopt the tartaric acid solution that 200ml concentration is 10% in step a and step b, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 200ml concentration is 0.25% and 200ml concentration are 0.15%.
7. according to the detection method of the described a kind of food liquid nitrite of claim 5 or 6, it is characterized in that: the tartaric acid solution adopted in the tartaric acid solution adopted in step b, sulfanilic acid solution and hydrochloride naphthodiamide solution and step a, sulfanilic acid solution and hydrochloride naphthodiamide solution phase are same.
8. the detection method of a kind of food liquid nitrite according to claim 1, it is characterized in that: after step a carried out once, the method only includes:
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, with the 100ml concentration tartaric acid solution that is 20%, the hydrochloride naphthodiamide solution that the sulfanilic acid solution that 100ml concentration is 0.5% and 100ml concentration are 0.3% mixes, pouring distilled water to solution quality into is 1kg again, after abundant mixed shaking, to the solution imaging, get red figure layer with colored CCD or CMOS, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
The gray-scale value obtained according to step b, the gray-scale value that contrast step a obtains-sodium nitrite figure-of-merit curve, calculate the contained content of sodium nitrite of 0.5kg food liquid to be measured.
9. the detection method of a kind of food liquid nitrite according to claim 1 is characterized in that: described food liquid is milk, wine, beverage, congee, scald, medicine soup.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277680A (en) * | 2014-07-03 | 2016-01-27 | 北京倍肯华业科技发展有限公司 | Intelligentized immunochromatography and spectral analysis integrated device and analysis method therefor |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61142443A (en) * | 1984-12-17 | 1986-06-30 | Matsushita Electric Ind Co Ltd | Method and apparatus for detecting nitrous acid ion |
| JPH0650952A (en) * | 1992-07-29 | 1994-02-25 | Shimadzu Corp | Nitrous acid ion analyzer |
| CN1320816A (en) * | 2000-04-24 | 2001-11-07 | 攀枝花市卫生防疫站 | Rapid detection method of nitrite in food |
| CN1472527A (en) * | 2002-08-02 | 2004-02-04 | ƽ | Multiple-marknig-object biological chip signal analyzing systems |
| CN101852740A (en) * | 2010-05-16 | 2010-10-06 | 丁宗庆 | Colorimetric trace analysis method of solvent extraction enrichment-solid matrix imaging |
| CN101893575A (en) * | 2010-04-08 | 2010-11-24 | 新疆农业大学 | Method for rapidly testing nitrite in fresh milk |
| CN102650590A (en) * | 2011-02-24 | 2012-08-29 | 北京吉天仪器有限公司 | Method for determining content of nitrogen in nitrate and/or nitrite of water sample and device thereof |
| CN103018232A (en) * | 2011-09-20 | 2013-04-03 | 内蒙古蒙牛乳业（集团）股份有限公司 | Detection method for sulfite in milk |
| CN103063663A (en) * | 2012-12-24 | 2013-04-24 | 大连大学 | Testing method for total phosphorus content in soil based on image analysis |
-
2013
- 2013-10-14 CN CN201310478133.5A patent/CN103487386B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61142443A (en) * | 1984-12-17 | 1986-06-30 | Matsushita Electric Ind Co Ltd | Method and apparatus for detecting nitrous acid ion |
| JPH0650952A (en) * | 1992-07-29 | 1994-02-25 | Shimadzu Corp | Nitrous acid ion analyzer |
| CN1320816A (en) * | 2000-04-24 | 2001-11-07 | 攀枝花市卫生防疫站 | Rapid detection method of nitrite in food |
| CN1472527A (en) * | 2002-08-02 | 2004-02-04 | ƽ | Multiple-marknig-object biological chip signal analyzing systems |
| CN101893575A (en) * | 2010-04-08 | 2010-11-24 | 新疆农业大学 | Method for rapidly testing nitrite in fresh milk |
| CN101852740A (en) * | 2010-05-16 | 2010-10-06 | 丁宗庆 | Colorimetric trace analysis method of solvent extraction enrichment-solid matrix imaging |
| CN102650590A (en) * | 2011-02-24 | 2012-08-29 | 北京吉天仪器有限公司 | Method for determining content of nitrogen in nitrate and/or nitrite of water sample and device thereof |
| CN103018232A (en) * | 2011-09-20 | 2013-04-03 | 内蒙古蒙牛乳业（集团）股份有限公司 | Detection method for sulfite in milk |
| CN103063663A (en) * | 2012-12-24 | 2013-04-24 | 大连大学 | Testing method for total phosphorus content in soil based on image analysis |
Non-Patent Citations (4)
| Title |
|---|
| 庞琳 等: ""基于机器视觉系统测量有色溶液浓度的方法研究"", 《东北电力学院学报》 * |
| 林海棠 等: ""提高CCD 糖液浓度测量系统精度的方法"", 《机电工程》 * |
| 王亚坤: ""基于机器视觉的萃取过程铜离子浓度软测量"", 《中国优秀硕士学位论文全文数据库 信息科技辑》 * |
| 王孟歌，侯苗联: ""奶制品中亚硝酸盐含量的测定"", 《河北化工》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277680A (en) * | 2014-07-03 | 2016-01-27 | 北京倍肯华业科技发展有限公司 | Intelligentized immunochromatography and spectral analysis integrated device and analysis method therefor |
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