CN103487386B - A kind of detection method of nitrate content in liquid food - Google Patents
A kind of detection method of nitrate content in liquid food Download PDFInfo
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Abstract
The detection method of nitrate content in liquid food belongs to a field of detection of food safety, is specifically related to a kind of detection method of nitrate content in liquid food; First the method is set up content of nitrite and is measured curve, then configures food liquid detection solution, is measured the corresponding relation of curve, obtain food liquid content of nitrite to be measured by the red layer gray-scale value of food liquid detection solution image and content of nitrite; The present invention provide not only a kind of new content of nitrite assay method, and adopts machine vision means, the method for indirect inspection content of nitrite, easy to operate, is convenient to popularize, therefore also has the fast advantage of measuring speed.
Description
Technical field
The detection method of nitrate content in liquid food belongs to a field of detection of food safety, is specifically related to a kind of detection method of nitrate content in liquid food.
Background technology
Nitrite, the general name of a class mineral compound.Main finger sodium nitrite, sodium nitrite is white to pale yellow powder or graininess, and taste is micro-salty, soluble in water.Outward appearance and flavour are all similar to salt, and widely use in industry, building industry, also allow to use as colour former limitation in meat product.
But, some illegal retailers, in order to the object of getting a profit by fair means or foul, in food, add nitrite goods, cause food-safety problem.Nitrite directly acts on the blood vessel of people, peripheral vasodilation can be caused, and methemoglobin can be formed with haemoglobin in blood, occur dizzy, the Nausea and vomiting even clinical symptoms such as blood pressure drops or shock, the nitrite eating 0.3 ~ 0.5 gram can cause poisoning even dead.More seriously, nitrite is typical carcinogenic substance simultaneously.Therefore, in order to ensure that consumer is healthy, be necessary to carry out strict detection and control to smoked sauce content of nitrite in foods.
Traditional nitrite detection method step is complicated, and length consuming time, and patent of invention " a kind of method of Raw Milk nitrite ", application number: 201010141449.1, can content of nitrite in Raw Milk, this method, can be used for other food liquid of direct-detection.But this method can only detect whether contain nitrite goods, and can not determine its exact level.
Summary of the invention
In order to solve the problem, the present invention devises a kind of detection method of nitrate content in liquid food, provide not only a kind of new content of nitrite assay method, and adopt machine vision means, the method of indirect inspection content of nitrite, easy to operate, be convenient to popularize, therefore also there is the fast advantage of measuring speed.
The object of the present invention is achieved like this:
A detection method for nitrate content in liquid food, comprises the following steps:
Step a, set up content of nitrite measure curve
Get sodium nitrite 1mg, 2mg, 3mg, 4mg and 5mg respectively, be dissolved in the tartaric acid solution that 100ml concentration is 20%, 100ml concentration be 0.5% sulfanilic acid solution and 100ml concentration be the mixed liquor of the hydrochloride naphthodiamide solution of 0.3%, in 5 mixed liquors, pour distilled water to solution quality respectively into is 1kg, after fully mixed shaking, the pink of different gray scale will be there is in solution, with colored CCD or CMOS to solution imaging, get red layer, record its gray-scale value, and draw gray-scale value-sodium nitrite figure-of-merit curve;
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, be the tartaric acid solution of 20% with 100ml concentration, 100ml concentration be 0.5% sulfanilic acid solution and 100ml concentration be 0.3% hydrochloride naphthodiamide solution mixing, pouring distilled water to solution quality again into is 1kg, after fully mixed shaking, with colored CCD or CMOS to solution imaging, get red layer, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
According to the gray-scale value that step b obtains, gray-scale value-sodium nitrite figure-of-merit curve that contrast step a obtains, calculates the content of sodium nitrite contained by 0.5kg food liquid to be measured.
The detection method of above-mentioned a kind of nitrate content in liquid food, also comprises steps d: content of sodium nitrite × 2 obtained by step c, obtains the content of sodium nitrite contained by 1kg food liquid to be measured.
The detection method of above-mentioned a kind of nitrate content in liquid food, the gray-scale value in described step a-sodium nitrite figure-of-merit curve, adjacent 2 is straight line connection.
The detection method of above-mentioned a kind of nitrate content in liquid food, the gray-scale value in described step a-sodium nitrite figure-of-merit curve is 5 Function Fitting curves.
The detection method of above-mentioned a kind of nitrate content in liquid food, can also adopt in step a and step b 50ml concentration be 40% tartaric acid solution, 50ml concentration be 1% sulfanilic acid solution and 50ml concentration be 0.6% hydrochloride naphthodiamide solution.
The detection method of above-mentioned a kind of nitrate content in liquid food, can also adopt in step a and step b 200ml concentration be 10% tartaric acid solution, 200ml concentration be 0.25% sulfanilic acid solution and 200ml concentration be 0.15% hydrochloride naphthodiamide solution.
The tartaric acid solution adopted in step b, sulfanilic acid solution and hydrochloride naphthodiamide solution are identical with the tartaric acid solution adopted in step a, sulfanilic acid solution and hydrochloride naphthodiamide solution.
The detection method of above-mentioned a kind of nitrate content in liquid food, when step a performed once, the method only includes:
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, be the tartaric acid solution of 20% with 100ml concentration, 100ml concentration be 0.5% sulfanilic acid solution and 100ml concentration be 0.3% hydrochloride naphthodiamide solution mixing, pouring distilled water to solution quality again into is 1kg, after fully mixed shaking, with colored CCD or CMOS to solution imaging, get red layer, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
According to the gray-scale value that step b obtains, gray-scale value-sodium nitrite figure-of-merit curve that contrast step a obtains, judges the content of sodium nitrite contained by 0.5kg food liquid to be measured.
The detection method of above-mentioned a kind of nitrate content in liquid food, described food liquid is milk, wine, beverage, congee, boiling hot, medicine soup.
First detection method due to nitrate content in liquid food of the present invention is set up content of nitrite and is measured curve, configure food liquid again and detect solution, measured the corresponding relation of curve by the red layer gray-scale value of food liquid detection solution image and content of nitrite, obtain food liquid content of nitrite to be measured; The present invention provide not only a kind of new content of nitrite assay method, and adopts machine vision means, the method for indirect inspection content of nitrite, easy to operate, is convenient to popularize, therefore also has the fast advantage of measuring speed.
Accompanying drawing explanation
Fig. 1 is the detection method process flow diagram of nitrate content in liquid food of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the invention is described in further detail.
Specific embodiment one
The detection method of the nitrate content in liquid food of the present embodiment, as shown in Figure 1, the method comprises the following steps process flow diagram:
Step a, set up content of nitrite measure curve
Get sodium nitrite 1mg respectively, 2mg, 3mg, 4mg and 5mg, be dissolved in the tartaric acid solution that 100ml concentration is 20%, 100ml concentration be 0.5% sulfanilic acid solution and 100ml concentration be the mixed liquor of the hydrochloride naphthodiamide solution of 0.3%, in 5 mixed liquors, pour distilled water to solution quality respectively into is 1kg, after fully mixed shaking, the pink of different gray scale will be there is in solution, with colored CCD or CMOS to solution imaging, get red layer, record its gray-scale value, and draw gray-scale value-sodium nitrite figure-of-merit curve, two-point defined line adjacent in gray-scale value-sodium nitrite figure-of-merit curve connects,
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, be the tartaric acid solution of 20% with 100ml concentration, 100ml concentration be 0.5% sulfanilic acid solution and 100ml concentration be 0.3% hydrochloride naphthodiamide solution mixing, pouring distilled water to solution quality again into is 1kg, after fully mixed shaking, with colored CCD or CMOS to solution imaging, get red layer, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
According to the gray-scale value that step b obtains, gray-scale value-sodium nitrite figure-of-merit curve that contrast step a obtains, judges the content of sodium nitrite contained by 0.5kg food liquid to be measured.
Such as, when sodium nitrite quality is respectively 1mg, 2mg, 3mg, 4mg and 5mg, gray-scale value corresponding to step a is respectively 10,20,32,46,60, the gray-scale value obtained as step b is 15, just between step a gray-scale value 10 and 20, therefore obtained sodium nitrite is between 1mg and 2mg, is 1.5mg, i.e. 1.5mg/ jin.
Specific embodiment two
The present embodiment and specific embodiment one unlike, the present embodiment also comprises steps d: content of sodium nitrite × 2 obtained by step c, obtains the content of sodium nitrite contained by 1kg food liquid to be measured.Utilize the data of specific embodiment one, finally detecting the content of sodium nitrite obtained is 3mg/kg.
Specific embodiment three
The present embodiment and specific embodiment one unlike, gray-scale value in the present embodiment step a-sodium nitrite figure-of-merit curve, be 5 Function Fitting curves, although 5 Function Fitting calculated amount are slightly large, the situation that the relation between gray-scale value and sodium nitrite quality of being more suitable for is linearly poor.
Specific embodiment four
The present embodiment and different being of embodiment before, can also adopt in step a and step b 50ml concentration be 40% tartaric acid solution, 50ml concentration be 1% sulfanilic acid solution and 50ml concentration be 0.6% hydrochloride naphthodiamide solution.The tartaric acid solution adopted in step b, sulfanilic acid solution and hydrochloride naphthodiamide solution are identical with the tartaric acid solution adopted in step a, sulfanilic acid solution and hydrochloride naphthodiamide solution.
Specific embodiment five
The present embodiment and different being of embodiment before, can also adopt in step a and step b 200ml concentration be 10% tartaric acid solution, 200ml concentration be 0.25% sulfanilic acid solution and 200ml concentration be 0.15% hydrochloride naphthodiamide solution.The tartaric acid solution adopted in step b, sulfanilic acid solution and hydrochloride naphthodiamide solution are identical with the tartaric acid solution adopted in step a, sulfanilic acid solution and hydrochloride naphthodiamide solution.
Specific embodiment six
The detection method of the nitrate content in liquid food of the present embodiment, when step a performed once, the method only includes:
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, be the tartaric acid solution of 20% with 100ml concentration, 100ml concentration be 0.5% sulfanilic acid solution and 100ml concentration be 0.3% hydrochloride naphthodiamide solution mixing, pouring distilled water to solution quality again into is 1kg, after fully mixed shaking, with colored CCD or CMOS to solution imaging, get red layer, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
According to the gray-scale value that step b obtains, gray-scale value-sodium nitrite figure-of-merit curve that contrast step a obtains, judges the content of sodium nitrite contained by 0.5kg food liquid to be measured.
The detection method of the nitrate content in liquid food of above embodiment, described food liquid comprises milk, wine, beverage, congee, boiling hot, medicine soup.
The present invention is not limited to above-mentioned preferred forms, and anyone should learn the structure change or method improvement made under enlightenment of the present invention, and every have identical or close technical scheme with the present invention, all falls within protection scope of the present invention.
Claims (3)
1. a detection method for nitrate content in liquid food, is characterized in that: comprise the following steps:
Step a, set up content of nitrite measure curve
Get sodium nitrite 1mg, 2mg, 3mg, 4mg and 5mg respectively, be dissolved in the tartaric acid solution that 100ml concentration is 20%, 100ml concentration be 0.5% sulfanilic acid solution and 100ml concentration be the mixed liquor of the hydrochloride naphthodiamide solution of 0.3%, in 5 mixed liquors, pour distilled water to solution quality respectively into is 1kg, after fully mixed shaking, the pink of different gray scale will be there is in solution, with colored CCD or CMOS to solution imaging, get red layer, record its gray-scale value, and draw gray-scale value-sodium nitrite figure-of-merit curve;
Described gray-scale value-sodium nitrite figure-of-merit curve is 5 Function Fitting curves;
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, be the tartaric acid solution of 20% with 100ml concentration, 100ml concentration be 0.5% sulfanilic acid solution and 100ml concentration be 0.3% hydrochloride naphthodiamide solution mixing, pouring distilled water to solution quality again into is 1kg, after fully mixed shaking, with colored CCD or CMOS to solution imaging, get red layer, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
According to the gray-scale value that step b obtains, gray-scale value-sodium nitrite figure-of-merit curve that contrast step a obtains, calculates the content of sodium nitrite contained by 0.5kg food liquid to be measured.
2. the detection method of a kind of nitrate content in liquid food according to claim 1, is characterized in that: when step a performed once, the method only includes:
Step b, configuration food liquid detect solution
Get food liquid 0.5kg to be measured, be the tartaric acid solution of 20% with 100ml concentration, 100ml concentration be 0.5% sulfanilic acid solution and 100ml concentration be 0.3% hydrochloride naphthodiamide solution mixing, pouring distilled water to solution quality again into is 1kg, after fully mixed shaking, with colored CCD or CMOS to solution imaging, get red layer, record its gray-scale value;
Step c, calculate food liquid content of nitrite to be measured
According to the gray-scale value that step b obtains, gray-scale value-sodium nitrite figure-of-merit curve that contrast step a obtains, calculates the content of sodium nitrite contained by 0.5kg food liquid to be measured.
3. the detection method of a kind of nitrate content in liquid food according to claim 1, is characterized in that: described food liquid is milk, wine, beverage, congee, boiling hot, medicine soup.
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| JPH0650952A (en) * | 1992-07-29 | 1994-02-25 | Shimadzu Corp | Nitrous acid ion analyzer |
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| CN1255671C (en) * | 2002-08-02 | 2006-05-10 | 上海数康生物科技有限公司 | Multiple-marknig-object biological chip signal analyzing systems |
| CN101893575A (en) * | 2010-04-08 | 2010-11-24 | 新疆农业大学 | Method for rapidly testing nitrite in fresh milk |
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| CN101852740A (en) * | 2010-05-16 | 2010-10-06 | 丁宗庆 | Colorimetric trace analysis method of solvent extraction enrichment-solid matrix imaging |
| CN103063663A (en) * | 2012-12-24 | 2013-04-24 | 大连大学 | Testing method for total phosphorus content in soil based on image analysis |
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