CN104677901A - Cereal detection reagent as well as detection method and application thereof - Google Patents
Cereal detection reagent as well as detection method and application thereof Download PDFInfo
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- CN104677901A CN104677901A CN201510148156.9A CN201510148156A CN104677901A CN 104677901 A CN104677901 A CN 104677901A CN 201510148156 A CN201510148156 A CN 201510148156A CN 104677901 A CN104677901 A CN 104677901A
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Abstract
The invention provides a cereal detection reagent as well as a detection method and application thereof. The cereal detection reagent comprises the following components in percentage by weight: 0.01%-0.05% of methyl red, 0.03%-0.15% of bromothymol blue, 0.01%-0.03% of mycose, 0.01%-0.05% of sodium hydroxide, 0.001%-0.003% of sodium dodecyl sulfate, 30% by volume of ethanol and the balance of water. The detection method comprises the following steps: (1) weighing proper components, and dissolving, so as to prepare the detection reagent; and (2) putting to-be-detected cereal samples on horizontally laid paper, spraying the detection reagent to the cereals once or twice, and observing the color change of the cereals within 1 minute. The invention provides application of the cereal detection reagent in cereal detection. According to the cereal detection reagent, cereals do not need to be soaked, and the color change of the cereals can be observed by virtue of a direct spraying manner, so that the operation is relatively simple and convenient, and the detection range is wide.
Description
Technical field
The invention belongs to field of detection of food safety, be specifically related to a kind of cereal and detect reagent and detection method thereof and application.
Background technology
Cereal is along with the prolongation of normal storage fresh keeping time, ageing gradually, as food is obviously deteriorated, color and luster is dimmed, fragrance disappears, acidity increases, occur that furancarboxylic acid taste and viscosity decline, water absorbing capacity reduces, retention ability goes down, cook congee the problems such as not thick soup, edible quality reduction.Paddy in storage whether ageing must the fatty acid value of paddy, viscosity, trial test score value, color and luster, smell be detected contrast " grain and oil storage quality decision rule ", wherein there is one to reach freshness index in fatty acid value, viscosity, trial test score value three, be then judged to be grain in storage for years.At present clear and definite national standard be there is no to the storage quality Con trolling index of rice.
The method of existing detection rice new-old degree has TBA determination method, acid base indicator method, enzymatic activity method, pH meter, TBA determination method, pH meter, these method test steps are complicated, complex operation, cannot judge newly oldly to mix rice, need equipment to assist detection, enzymatic activity method and acid base indicator method require that solution now joins first use, are not suitable for on-site law-enforcing and detect and common people's routine use.And above several method is all not suitable for judging polished rice.
Summary of the invention
Therefore, the object of this invention is to provide a kind of cereal and detect reagent and detection method thereof and application, this detection reagent for detect operation simpler, detect more convenient, without the need to other utility appliance, develop the color more stable, homogeneous, thus can more convenient, detect cereal rapidly.
For above-mentioned purpose, technical scheme provided by the invention is as follows:
On the one hand, the invention provides a kind of cereal and detect reagent, described detection reagent comprises each component of following percentage by weight: methyl red 0.01-0.05%, Bromothymol blue 0.03-0.15%, trehalose 0.01-0.03%, NaOH 0.01-0.05%, sodium dodecylsulphonate 0.001-0.003%, ethanol also containing 30 volume %, surplus is water.
Preferably, described detection reagent comprises each component of following percentage by weight: methyl red 0.02%, Bromothymol blue 0.06%, trehalose 0.015%, NaOH 0.03%, sodium dodecylsulphonate 0.001%.
Preferably, described cereal comprises rice and/or millet.
Preferably, described rice comprises new old doping rice and/or polishing rice.
Preferably, described millet is new-old degree doping millet.
On the other hand, the present invention also provides a kind of cereal of the present invention to detect the detection method of reagent, comprises the following steps:
1) weigh methyl red, Bromothymol blue, trehalose, NaOH, sodium dodecylsulphonate and absolute ethyl alcohol by suitable weight percent content, add suitable quantity of water ultrasonic dissolution, obtained each feed composition mixed liquor;
2) then, the NaOH weighing suitable percentage by weight adds in described each feed composition mixed liquor, then the constant volume that adds water, mixing, obtained detection reagent;
2) getting grain sample to be detected is placed on the paper of tiling, under described detection reagent being aimed at cereal sprinkling 1-2, then in 1 minute, observes grain colour change.
Preferably, when described cereal be new old doping rice and/or polishing rice time, spraying green after reagent or the yellowish green grain of rice is new rice, and the orange-red grain of rice is old rice, and green purer expression rice is fresher, and orange more deeply feeling shows that rice is older.
Preferably, when described cereal is new old doping millet, spray after reagent, the red grain of rice is old millet, yellow and millet intrinsic colour be new millet, redness is more deeply felt and is shown that millet is older.
Also on the one hand, the present invention also provides a kind of cereal of the present invention to detect the application of reagent in detecting for cereal.
Preferably, described cereal detects the detection comprising the new detection of old doping rice, the identification of polishing rice and new old doping millet.
Detection reagent of the present invention and detection method do not need cereal immersion treatment, directly adopt the mode of spray, contrast grain colour immediately to change, make operation simpler, detect more convenient, without the need to other utility appliance, save the running time, not only may be used for detecting rice, also can be used for detecting millet, and can perform well in detecting polishing rice; In addition, sodium dodecylsulphonate, due to its good infiltration, wetting, emulsification, can react with the free fatty acid on surface fast after making reagent act on rice surface, and colour developing can be made homogeneous.Trehalose makes colour developing more stable due to functional characters such as its good moisture retention, stability; And this reagent and method not only can detect rice can also detect millet, and sensing range is wide.
In summary of the invention, introduce the concept of a series of reduced form, this will further describe in embodiment part.Content part of the present invention does not also mean that the key feature and essential features that will attempt to limit technical scheme required for protection, does not more mean that the protection domain attempting to determine technical scheme required for protection.
Below in conjunction with accompanying drawing, describe advantages and features of the invention in detail.
Accompanying drawing explanation
Following accompanying drawing of the present invention in this as a part of the present invention for understanding the present invention.Shown in the drawings of embodiments of the present invention and description thereof, be used for explaining principle of the present invention.In the accompanying drawings,
Fig. 1 a is the A rice detected is the result of new rice;
Fig. 1 b is the B rice detected is the result of old rice;
Fig. 1 c is the C rice detected is the result of the rice of new old doping;
Fig. 2 is the D rice detected is the result of polishing rice;
Fig. 3 a is the A millet detected is the result of new rice;
Fig. 3 b is the B millet detected is the result of old rice;
Fig. 3 c is the C millet detected is the result of the millet of new old doping.
Embodiment
In the following description, a large amount of details is provided the present invention can be understood up hill and dale.But those skilled in the art can understand, following description only relates to preferred embodiment of the present invention, and the present invention can be implemented without the need to one or more such details.In addition, in order to avoid obscuring with the present invention, technical characteristics more well known in the art are not described.
Embodiment 1
1) each component detecting below reagent percentage by weight is taken: methyl red 0.02%, Bromothymol blue 0.06%, trehalose 0.015% sodium dodecylsulphonate 0.001%, add absolute ethyl alcohol again, its content is made to account for the final 30 volume % detecting reagent, add water ultrasonic dissolution, obtains each feed composition mixed liquor;
2) then, the NaOH weighing 0.03% percentage by weight adds in described each feed composition mixed liquor, then the constant volume that adds water, mixing, obtained detection reagent.
3) be placed in respectively on the paper of tiling from the three parts of rice samples (every part of rice sample 15-50 grain) of getting commercially available A, B, C rice to be detected, under described detection reagent being aimed at rice sprinkling 1-2, the change of rice color is observed again in 1 minute, result as illustrated by figures 1 a-1 c, wherein in Fig. 1 a, spraying A rice after detecting reagent is green, yellow green (representing green by grey in figure), shows that this rice sample is new rice; In Fig. 1 b, after sprinkling detection reagent, B rice is orange red (representing orange red by white in figure), shows that this rice sample is old rice; In Fig. 1 c, spraying C rice part after detecting reagent is orange red (represent green by grey in figure, white represents orange red) for green or yellow green part, shows that this rice sample is new old doping rice.
In order to verify whether correct three parts of rice samples that above-described embodiment detects detect, adopt " grain of the prior art, oil plant is checked: color and luster, smell, taste identification method " (GB5492-85) method, again from A, B, get three parts of rice samples in C rice to detect, result proves A, B, C rice is respectively new rice, old rice and new old doping rice, consistent with above-described embodiment testing result, and by the rice sample that the method for the prior art detects, there is sensing range narrow, sensitivity is low, reagent needs extemporaneous preparation otherwise the shortcomings such as poor repeatability, illustrate that method of the present invention is better than detection method of the prior art.
Embodiment 2
1) each component detecting below reagent percentage by weight is taken: methyl red 0.01%, Bromothymol blue 0.03%, trehalose 0.03% sodium dodecylsulphonate 0.002%, add absolute ethyl alcohol again, its content is made to account for the final 30 volume % detecting reagent, add water ultrasonic dissolution, obtains each feed composition mixed liquor;
2) then, the NaOH weighing 0.01% percentage by weight adds in described each feed composition mixed liquor, then the constant volume that adds water, mixing, obtained detection reagent;
3) from the D rice of commercially available bright appearance to be detected, getting rice sample to be detected is placed on the paper of tiling, under described detection reagent being aimed at rice sprinkling 1-2, the change of rice color is observed in 1 minute, result as shown in Figure 2, the D rice sprayed after detecting reagent is orange red (representing orange red by white in figure), represents that it is the rice after old rice polishing.
In order to verify whether correct the rice sample that above-described embodiment detects detects, adopt the method for " painting stain grease and paraffin rice checking " (GB/T21309-2007) in prior art, again from D rice, get a rice sample detect, result proves that D rice is rice after polishing, consistent with above-described embodiment testing result.
Embodiment 3
1) each component detecting below reagent percentage by weight is taken: methyl red 0.05%, Bromothymol blue 0.15%, trehalose 0.02%, sodium dodecylsulphonate 0.003%, add absolute ethyl alcohol again, its content is made to account for the final 30 volume % detecting reagent, add water ultrasonic dissolution, obtains each feed composition mixed liquor;
2) then, the NaOH weighing 0.05% percentage by weight adds in described each feed composition mixed liquor, then the constant volume that adds water, mixing, obtained detection reagent; 3) get in commercially available A, B, C millet to be detected and get three parts of millet samples (every part of millet sample 15-50 grain) and be placed in respectively on the paper of tiling, under described detection reagent being aimed at rice sprinkling 1-2, the change of millet color is observed in 1min, result as shown in Figure 3 a, after spraying detection reagent, millet color does not become is still yellow (representing yellow by white in figure), shows that this millet is new rice; As shown in Figure 3 b, spraying millet color after detecting reagent is red (representing red by grey in figure), shows that this millet is old rice; As shown in Figure 3 c, spraying millet color after detecting reagent is part yl moiety redness (represent yellow by white in figure, represent red by grey), shows that this millet sample is the millet of new old doping.
Whether correct in order to verify three parts of millet sample detection that above-described embodiment detects, adopt the method for prior art " cereal products fatty acid value determination method " (GB/T15684-1995), again from A, B, C millet, get three parts of millet samples detect, result proves that A, B, C rice is respectively new rice, old rice and new old doping millet, consistent with above-described embodiment testing result.
Comparative example 1
By the detection method in embodiment 1, A, B, C tri-rice are detected, different lacks sodium dodecylsulphonate for detecting in reagent, result shows the rice colour developing heterogeneity that this detection reagent detects, the new-old degree of these three rice cannot be judged exactly, illustrate that the detection reagent after adding sodium dodecylsulphonate can more adequately detect the new-old degree of rice sample.
Comparative example 2
By the detection method in embodiment 3, A, B, C tri-millet samples are detected, different lacks trehalose for detecting in reagent, result shows, develop the color latter 10 minutes, redness in old rice has takes off phenomenon, colour developing is unstable, illustrates that the detection reagent after adding sodium dodecylsulphonate can more adequately detect the new-old degree of rice sample.
The present invention is illustrated by above-described embodiment, but should be understood that, above-described embodiment just for the object of illustrating and illustrate, and is not intended to the present invention to be limited in described scope of embodiments.In addition it will be appreciated by persons skilled in the art that the present invention is not limited to above-described embodiment, more kinds of variants and modifications can also be made according to instruction of the present invention, within these variants and modifications all drop on the present invention's scope required for protection.Protection scope of the present invention defined by the appended claims and equivalent scope thereof.
Claims (10)
1. a cereal detects reagent, described detection reagent comprises each component of following percentage by weight: methyl red 0.01-0.05%, Bromothymol blue 0.03-0.15%, trehalose 0.01-0.03%, NaOH 0.01-0.05% and sodium dodecylsulphonate 0.001-0.003%, ethanol also containing 30 volume %, surplus is water.
2. cereal according to claim 1 detects reagent, it is characterized in that, described detection reagent comprises each component of following percentage by weight: methyl red 0.02%, Bromothymol blue 0.06%, trehalose 0.015%, NaOH 0.03%, sodium dodecylsulphonate 0.001%.
3. cereal according to claim 1 and 2 detects reagent, and it is characterized in that, described cereal comprises rice and/or millet.
4. cereal according to claim 3 detects reagent, and it is characterized in that, described rice comprises new old doping rice and/or polishing rice.
5. cereal according to claim 3 detects reagent, it is characterized in that, described millet is new-old degree doping millet.
6. cereal according to any one of claim 1 to 5 detects the detection method of reagent, comprises the following steps:
1) weigh methyl red, Bromothymol blue, trehalose, sodium dodecylsulphonate and absolute ethyl alcohol by suitable weight percent content, add suitable quantity of water ultrasonic dissolution, obtained each feed composition mixed liquor;
2) subsequently, the NaOH weighing suitable percentage by weight adds in described each feed composition mixed liquor, then the constant volume that adds water, mixing, obtained detection reagent;
3) getting grain sample to be detected is placed on the paper of tiling, under described detection reagent being aimed at cereal sprinkling 1-2, then in 1 minute, observes grain colour change.
7. cereal according to claim 6 detects the detection method of reagent, it is characterized in that, when described cereal be new old doping rice and/or polishing rice time, after spraying reagent, the green or yellowish green grain of rice is new rice, and the orange-red grain of rice is old rice.
8. cereal according to claim 6 detects the detection method of reagent, it is characterized in that, when described cereal is new old doping millet, after spraying reagent, the red grain of rice is old millet, yellow and millet intrinsic colour be new millet.
9. cereal according to any one of claim 1 to 5 detects the application of reagent in detecting for cereal.
10. application according to claim 9, is characterized in that, described cereal detects the detection comprising the new detection of old doping rice, the identification of polishing rice and new old doping millet.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510148156.9A CN104677901A (en) | 2015-03-31 | 2015-03-31 | Cereal detection reagent as well as detection method and application thereof |
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| CN201510148156.9A CN104677901A (en) | 2015-03-31 | 2015-03-31 | Cereal detection reagent as well as detection method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424696A (en) * | 2016-01-05 | 2016-03-23 | 北京北大明德科技发展有限公司 | Detection test paper for fast distinguishing fresh degree of rice and detection method |
| CN105911056A (en) * | 2016-06-16 | 2016-08-31 | 时树松 | Grain fatty acid value detection reagent, detection method and application of detection reagent |
-
2015
- 2015-03-31 CN CN201510148156.9A patent/CN104677901A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424696A (en) * | 2016-01-05 | 2016-03-23 | 北京北大明德科技发展有限公司 | Detection test paper for fast distinguishing fresh degree of rice and detection method |
| CN105424696B (en) * | 2016-01-05 | 2018-12-28 | 北京北大明德科技发展有限公司 | Rapid identification paddy new old Test paper and detection method |
| CN105911056A (en) * | 2016-06-16 | 2016-08-31 | 时树松 | Grain fatty acid value detection reagent, detection method and application of detection reagent |
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Application publication date: 20150603 |