CN103529026A - Detection method and kit of illegal cooking oil as well as production method of detection color comparison card - Google Patents
Detection method and kit of illegal cooking oil as well as production method of detection color comparison card Download PDFInfo
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- CN103529026A CN103529026A CN201310498935.2A CN201310498935A CN103529026A CN 103529026 A CN103529026 A CN 103529026A CN 201310498935 A CN201310498935 A CN 201310498935A CN 103529026 A CN103529026 A CN 103529026A
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Abstract
The invention discloses a detection method and a kit of illegal cooking oil as well as a production method of a detection color comparison card. The detection method comprises the following steps: dripping detected oil onto a silica gel plate, spreading the detected oil on the silica gel plate through a developing agent, spraying a color developing agent for color development, comparing the color of the detected oil with a standard color comparison card, observing if the detected oil exceeds the standard according to the motion trail and the color change, and if the detected oil exceeds the standard, judging that the detected oil is fried illegal cooking oil, wherein the developing agent comprises petroleum ether, ethyl ether and acetic acid in a volume ratio of (60-70) to (20-30) to (5-10). The detection kit comprises the standard color comparison card, the G-type silica gel plate, the developing agent and the color developing agent. The method adopts a simple operation process and can be used for quickly and accurately identifying the illegal cooking oil.
Description
Technical field
The present invention relates to the technical field that waste oil detects, espespecially a kind of detection method of waste oil is, the detection kit of waste oil and waste oil detect the method for making with colorimetric card.
Background technology
Waste oil is food and drink waste residue leftovers, the dishwashing detergent water of the inflow trench collected by illegal retailer, the grease obtaining through simple oil and water separation, filtration, decolouring supervisor, its physical and chemical indexes as: acid value, peroxide value etc. are with respect to normal edible oil severe overweight, wherein contain the noxious materials such as aldehyde, ketone, aflatoxins, to health greatly infringement again.
In general, waste oil is divided into following three classes: the one, after surpassing regulation and require for the oily access times of fried food, then be reused or toward wherein adding the oil of reusing after some fresh oils; The 2nd, by the greasy floating thing in sewer or by the leftovers of hotel, restaurant, leftovers (common name swill) through simply processing, the oil extracting; The 3rd, by the oil of output after the processing such as pork inferior, haslet, pigskin and refinement.
The index that waste oil detects at present mainly contains moisture, index of refraction, saponification number, acid value, carbonyl value, volatile components, conductivity etc., therefore detection method is also a lot, such as: solidifying point detection method, transparency detection method, viscosity measurements method, refracting power detection method, the spectrum property surveyed detection method etc., but these detection methods all utilize instrument to detect according to character, exist a lot of deficiencies, as complicated in detecting step, checkout equipment expensive, consuming time long, lack applicability, inconvenient execute-in-place etc. widely.
In view of above-mentioned deficiency, this case inventor, for frying type waste oil, find the specific index that has more broad applicability, and by the least possible detection index, shirtsleeve operation flow process is as far as possible differentiated fast and accurately waste oil, and this case produces therefrom.
Summary of the invention
It is a kind of simple to operate that technical matters to be solved by this invention is to provide, and can detect fast, effectively, accurately the method for waste oil, the kit of detection waste oil.
Technical matters to be solved by this invention is to provide a kind of waste oil to detect the method for making of the colorimetric card of use.
For solving the problems of the technologies described above, technical solution of the present invention is:
A detection method for waste oil, will detect oil droplet on silica gel plate, by developping agent, make detection oil launch on silica gel plate, spray afterwards chromogenic reagent, whether comparison standard color comparison card, observe detection oil according to hangover and change color and exceed standard, if exceeded standard, is judged as frying type waste oil; The proportioning of described developping agent is sherwood oil by volume: ether: acetic acid is 60 ~ 70:20 ~ 30:5 ~ 10.
The proportioning of described developping agent is sherwood oil by volume: ether: acetic acid is 70:25:5.
Described developer is the chloroformic solution of 0.1 ~ 1% iodine.
Described silica gel plate is selected G type silica gel plate.
A detection kit for waste oil, comprises standard color comparison card, G type silica gel plate, developping agent and developer; The proportioning of described developping agent is sherwood oil by volume: ether: acetic acid is 60 ~ 70:20 ~ 30:5 ~ 10; Described developer is the chloroformic solution of 0.1 ~ 1% iodine.
Waste oil detects the method for making with colorimetric card, comprises the steps:
(1) preparation of frying oil: a certain amount of without fried fresh food crude oil to adding in cooking fryer, add again deep fried foodstuff to carry out fried, after fried ten minutes, stop fried and cooling ten minutes, so circulation is for twice one-period, during frying, note the stirring of deep fried foodstuff, after one-period, make oil temperature return back to room temperature; Afterwards, take out deep fried foodstuff, then add new deep fried foodstuff, repeat above operation, carry out the next cycle; So repeat several cycles, obtain frying oil;
(2) determine the critical oil sample of carbonyl valency: above-mentioned frying oil is mixed with the crude oil without frying, after dilution different multiples, obtain some oil samples, measure the carbonyl valency of each oil sample, obtain making typical curve after a series of data; According to the standard of the contained carbonyl valency of the frying oil of this curve and standard code, determine the dilution ratio of carbonyl valency when standard critical value;
(3) determining of standard color comparison card: crude oil and frying oil are diluted to the some oil samples of acquisition according to a certain percentage, in this oil sample, need to comprise the dilution ratio of above-mentioned carbonyl price when critical value, each oil sample is launched with developping agent on silica gel plate, obtain a series of colors after spraying developer; Choose the colour developing result of all or part of oil sample as the collection of illustrative plates standard of colorimetric card, be made into standard color comparison card;
The proportioning of described developping agent is sherwood oil by volume: ether: acetic acid is 60 ~ 70:20 ~ 30:5 ~ 10; Described developer is the chloroformic solution of 0.1 ~ 1% iodine; Described silica gel plate is selected G type silica gel plate.
Adopt after such scheme, the present invention selects this characteristic index of carbonyl valency in edible vegetable oil frying process, by developping agent, launch, use again chromogenic reagent, according to the standard color comparison card of hangover and change color and making in advance, compare afterwards, determine whether frying type waste oil, and developping agent and developer are determined best proportioning according to reality.The method has adopted shirtsleeve operation flow process, can differentiate fast and accurately waste oil.
Accompanying drawing explanation
Carbonyl valency curve map when Fig. 1 is frying oil of the present invention and crude oil dilution different proportion;
Fig. 2 is the asynchronous colour developing result schematic diagram of crude oil of the present invention and frying oil ratio;
Fig. 3 is the schematic diagram of standard color comparison card of the present invention.
Embodiment
Below the invention will be further described.
Disclosed is a kind of detection method of waste oil, to detect oil droplet on silica gel plate, by developping agent, make detection oil launch on silica gel plate, spray afterwards chromogenic reagent, comparison standard color comparison card, according to hangover and change color, observe detection oil and whether exceed standard, if exceeded standard, be judged as frying type waste oil.
The proportioning of described developping agent is sherwood oil by volume: ether: acetic acid is 60 ~ 70:20 ~ 30:5 ~ 10.Wherein best proportioning can be: sherwood oil: ether: acetic acid is 70:25:5.
Described silica gel plate can be selected G type silica gel plate.
Described developer can be selected the chloroformic solution of molybdophosphate ethanolic solution or iodine, but selecting the color that molybdophosphate ethanolic solution shows is aubergine, and color is darker, is difficult for observing.Therefore, through great many of experiments, select the chloroformic solution of 0.1 ~ 1% iodine, be revealed as yellow, and shade is moderate, easily observe.
The present invention also discloses a kind of detection kit of waste oil, comprises standard color comparison card, G type silica gel plate, developping agent and developer; Described developping agent adopts the best proportioning in said method, i.e. volume ratio sherwood oil: ether: acetic acid is 70:25:5.Can certainly adopt other proportionings in said method.Described developer can be the chloroformic solution of 0.1 ~ 1% iodine, the chloroformic solution of optimal selection 0.5% iodine.
Above-mentioned standard colorimetric calorie requirement completes in advance, and method for making is as follows:
1, the preparation of frying oil:
A certain amount of without fried fresh food crude oil to adding in cooking fryer, add again deep fried foodstuff to carry out fried, after fried ten minutes, stop fried and cooling ten minutes, so circulation is for twice one-period, during frying, note the stirring of deep fried foodstuff, after one-period, make oil temperature return back to room temperature; Afterwards, take out deep fried foodstuff, then add new deep fried foodstuff, repeat above operation, carry out the next cycle; So repeat 9 cycles, obtain frying oil.
2, determine the critical oil sample of carbonyl valency:
Above-mentioned frying oil is mixed with the crude oil without frying, after dilution different multiples, obtain some oil samples, measure the carbonyl valency of each oil sample, obtain making typical curve after a series of data, as shown in Figure 1.Method and the step of measuring carbonyl valency are the ordinary skill in the art, at this, are not described in detail.According to the carbonyl valency in regulation edible vegetable oil frying process in GB7102.1, should be less than or equal to 50meq/kg, according to curve shown in Fig. 1, determine that carbonyl valency is when 50meq/kg, the dilution ratio of crude oil and frying oil is 5.4:1, that is to say, when this ratio, be just positioned at the critical value of standard.
3, determining of standard color comparison card:
Crude oil and frying oil are diluted according to a certain percentage, and dilution ratio is followed successively by 1:0,9:1,8:1,7:1,6:1,5.4:1,4:1,3:1,2:1,1:1,0:1.Obtain 11 oil samples, each oil sample is launched with developping agent on silica gel plate, obtain a series of colors (as shown in Figure 2) after spraying developer.Described silica gel plate adopts G type silica gel plate, and described developping agent and developer all can adopt the above.
The colour developing result of the oil sample when ratio of choosing crude oil and frying oil is 1:0,9:1,7:1,5.4:1,3:1,1:1,0:1, as the collection of illustrative plates standard of colorimetric card, is made into standard color comparison card (as shown in Figure 3).
The above, be only preferred embodiment of the present invention, not technical scope of the present invention is imposed any restrictions, thus variation or the modification in every case according to claim of the present invention and instructions, done, within all should belonging to the scope that patent of the present invention contains.
Claims (6)
1. the detection method of a waste oil, it is characterized in that: will detect oil droplet on silica gel plate, by developping agent, make detection oil launch on silica gel plate, spray afterwards chromogenic reagent, comparison standard color comparison card, according to hangover and change color, observe detection oil and whether exceed standard, if exceeded standard, be judged as frying type waste oil; The proportioning of described developping agent is sherwood oil by volume: ether: acetic acid is 60 ~ 70:20 ~ 30:5 ~ 10.
2. the detection method of waste oil according to claim 1, is characterized in that: the proportioning of described developping agent is sherwood oil by volume: ether: acetic acid is 70:25:5.
3. the detection method of waste oil according to claim 1, is characterized in that: described developer is the chloroformic solution of 0.1 ~ 1% iodine.
4. the detection method of waste oil according to claim 1, is characterized in that: described silica gel plate is selected G type silica gel plate.
5. a detection kit for waste oil, is characterized in that: comprise standard color comparison card, G type silica gel plate, developping agent and developer; The proportioning of described developping agent is sherwood oil by volume: ether: acetic acid is 60 ~ 70:20 ~ 30:5 ~ 10; Described developer is the chloroformic solution of 0.1 ~ 1% iodine.
6. waste oil detects the method for making with colorimetric card, it is characterized in that comprising the steps:
(1) preparation of frying oil: a certain amount of without fried fresh food crude oil to adding in cooking fryer, then it is fried to add deep fried foodstuff to carry out, after fried ten minutes, stop fried and cooling ten minutes, so circulation is one-period twice, after one-period, makes oil temperature return back to room temperature; Afterwards, take out deep fried foodstuff, then add new deep fried foodstuff, repeat above operation, carry out the next cycle; So repeat several cycles, obtain frying oil;
(2) determine the critical oil sample of carbonyl valency: above-mentioned frying oil is mixed with the crude oil without frying, after dilution different multiples, obtain some oil samples, measure the carbonyl valency of each oil sample, obtain making typical curve after a series of data; According to the standard of the contained carbonyl valency of the frying oil of this curve and standard code, determine the dilution ratio of carbonyl valency when standard critical value;
(3) determining of standard color comparison card: crude oil and frying oil are diluted to the some oil samples of acquisition according to a certain percentage, in this oil sample, need to comprise the dilution ratio of above-mentioned carbonyl price when critical value, each oil sample is launched with developping agent on silica gel plate, obtain a series of colors after spraying developer; Choose the colour developing result of all or part of oil sample as the collection of illustrative plates standard of colorimetric card, be made into standard color comparison card;
The proportioning of described developping agent is sherwood oil by volume: ether: acetic acid is 60 ~ 70:20 ~ 30:5 ~ 10; Described developer is the chloroformic solution of 0.1 ~ 1% iodine; Described silica gel plate is selected G type silica gel plate.
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Cited By (5)
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| CN104459010A (en) * | 2014-12-13 | 2015-03-25 | 广西科技大学 | Method for qualitatively detecting waste cooking oil |
| CN104749176A (en) * | 2015-03-25 | 2015-07-01 | 淮海工学院 | New device and method for rapidly identifying illegal cooking oil by use of discoloration method |
| CN104807775A (en) * | 2015-01-29 | 2015-07-29 | 湖南省农产品加工研究所 | NIR spectrum analysis model and method used for identifying frying oil quality |
| CN105911056A (en) * | 2016-06-16 | 2016-08-31 | 时树松 | Grain fatty acid value detection reagent, detection method and application of detection reagent |
| CN110736742A (en) * | 2018-07-18 | 2020-01-31 | 郑州科技学院 | fried vegetable oil quality identification color developing agent and preparation method and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104459010A (en) * | 2014-12-13 | 2015-03-25 | 广西科技大学 | Method for qualitatively detecting waste cooking oil |
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| CN104807775A (en) * | 2015-01-29 | 2015-07-29 | 湖南省农产品加工研究所 | NIR spectrum analysis model and method used for identifying frying oil quality |
| CN104749176A (en) * | 2015-03-25 | 2015-07-01 | 淮海工学院 | New device and method for rapidly identifying illegal cooking oil by use of discoloration method |
| CN105911056A (en) * | 2016-06-16 | 2016-08-31 | 时树松 | Grain fatty acid value detection reagent, detection method and application of detection reagent |
| CN110736742A (en) * | 2018-07-18 | 2020-01-31 | 郑州科技学院 | fried vegetable oil quality identification color developing agent and preparation method and application thereof |
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Application publication date: 20140122 |