CN105424696A - Detection test paper for fast distinguishing fresh degree of rice and detection method - Google Patents
Detection test paper for fast distinguishing fresh degree of rice and detection method Download PDFInfo
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- CN105424696A CN105424696A CN201610001843.2A CN201610001843A CN105424696A CN 105424696 A CN105424696 A CN 105424696A CN 201610001843 A CN201610001843 A CN 201610001843A CN 105424696 A CN105424696 A CN 105424696A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N2021/786—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour with auxiliary heating for reaction
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Abstract
The invention discloses detection test paper for fast distinguishing the fresh degree of rice and a detection method. The detection test paper for fast distinguishing the fresh degree of rice is a test strip dried in air after being dipped in detection liquid, wherein the detection liquid contains colorless pinkish red, mercuric chloride and sulfuric acid, and the colorless pinkish red is prepared by introducing SO2 into a pinkish red solution until the solution becomes colorless. During detection, a user only needs to add water into rice manufactured from unhusked rice, steam the rice in a sealed mode, detect steam with the test paper and observe the color change of the test paper, if the test paper keeps colorless, it is indicated that the rice is fresh, and the redder the test paper is, the more stale the rice is. The distinguishing accuracy rate of the method is high, the accuracy of distinguishing the fresh degree of the rice is larger than 90%, in the distinguishing process, the method and operation are simple, and the method for qualitatively determining the fresh degree of the rice is reliable and effective.
Description
Technical field
The present invention relates to technical field of food safety detection, the Test paper that particularly a kind of rapid identification paddy is newly old and detection method.
Background technology
Rice is one of maximum staple food grain kind in the whole world, is also Chinese most important staple food grain kind.Its quality quality is directly connected to the food security of common people and healthy.
Rice supplies except consumer except processing then, directly, and major part needs to store with paddy form the lasting supply realizing grain, and storage time, length can reach several years, and short also has some months.One of feature of paddy to store, and under general storage condition, paddy Second Year just starts Deterioration, and some chemical compositions shown as in rice endosperm change.Free fatty acid can increase, starch composition cell membrane hardens, the composition structure of the grain of rice changes thereupon.
The key distinction of new old rice is the rice cooked, and its smell difference is huge, and the amount that this reason is wherein exactly the volatile matter such as aldehydes directly affects the local flavor of rice.The aldehyde material burst size of rice in digestion process that new paddy is produced is low, the distinctive aromatic flavour of rice, and the old rice that the resting period is longer, along with the prolongation of assembly time, aldehyde material (is mainly acetaldehyde, valeral and hexanal) volatile quantity raises gradually, so it is thin out to smell meter fragrance, peculiar smell or even mould obviously spoiled.
The mouthfeel of old rice and nutritive value are obviously poor than new rice, and many illegal dealer, in order to improve profit margin, pretend to be new rice after just the old rice polishing of outmoded paddy processing being waxed, cause being difficult in adoption process identify, thus obtain unlawful interests.The inspection of the new Chen Chengdu of paddy is a difficult problem for puzzlement food and beverage enterprise and food enterprise always.Based on present market situation, in the urgent need to finding the newly old way of a kind of quick detection rice.
Chinese patent application publication No. CN103364515A(date of publication 2013.10.23) disclose a kind of method detecting aldehyde material in rice, that the method adopting head space to derive solid-phase microextraction-gas chromatography combined with mass spectrometry detects, although accuracy is better, but need specific installation and environment, general place is difficult to meet, and detection time is long, detects and once need nearly two hours.Chinese patent application publication No. CN103454272A(date of publication 2013.12.18) disclose a kind of test paper of quick judgement rice new-old degree, be infiltrate the test strips having the hydrochloric acid-methanol solution of DNPH.
Summary of the invention
The object of the present invention is to provide a kind of Test paper easy to use, can judge the rice storage time by observing dye levels, Detection accuracy is high, present invention also offers a kind of detection method, simple to operate, application scenarios is almost unrestricted, and detection rates is fast.
The Test paper of rapid identification rice new-old provided by the invention, be the test strips of drying after immersion detection liquid, described detection liquid contains colourless magenta, mercuric chloride and sulfuric acid; Described colourless magenta passes into SO in fuchsin solution
2form to colourless.
The aldehydes release characteristics of the present invention's application rice identifies the new old property of paddy, and then qualitatively judges the storage time of paddy or rice.By studying the detection of aldehyde material in rice, finding the method that can detect, distinguish the new Chen Chengdu of paddy raw material, being convenient to the newness degree of rapid identification paddy in adoption process.
The new old property of paddy distinguishes the burst size mainly referring to the aldehyde material of rice in digestion process (acetaldehyde, valeral, hexanal).The aldehyde material burst size of outmoded paddy is comparatively large, and by aldehyde material agent for capturing mercuric chloride, under the catalytic action of sulfuric acid, aldehyde radical and sulphuric dioxide generation oxidation reaction, consume sulphuric dioxide, just reverted to redness before this by the fuchsin solution that sulphuric dioxide is bleached.Aldehydes quality is more, and the sulphuric dioxide that reaction consumes is more, and solution colour is redder.Fresh paddy, because aldehyde material burst size is very little, substantially can not react sulphuric dioxide, therefore fuchsin solution also can not revert to redness.During rice storage, its aldehyde material burst size can change, and therefore, can qualitatively judge newness degree or the storage time of paddy according to the depth degree of magenta color (depth of test paper redness).
Preferably, the Test paper that above-mentioned rapid identification rice is newly old, described detection liquid is mixed by colourless fuchsin solution, mercuric chloride solution and the sulfuric acid solution volume ratio according to 1:1:5 ~ 10, wherein pinkish red in colourless fuchsin solution mass concentration is 0.01%, in mercuric chloride solution, the concentration of mercuric chloride is 0.02 ~ 0.08mol/L, and in sulfuric acid solution, the concentration of sulfuric acid is 0.02 ~ 0.08mol/L.
Preferably, the Test paper that above-mentioned rapid identification rice is newly old, in described mercuric chloride solution, the concentration of mercuric chloride is 0.05mol/L, and in sulfuric acid solution, the concentration of sulfuric acid is 0.05mol/L; The volume ratio of colourless fuchsin solution, mercuric chloride solution and sulfuric acid solution is 1:1:10.
Preferably, the Test paper that above-mentioned rapid identification rice is newly old, described test strips is filter paper, and dip time is 1 ~ 2 minute, dries in the cool.Preferred dipping 1 minute.
The method that a kind of rapid identification paddy provided by the invention is newly old, it is characterized in that, get appropriate paddy processing and become edible rice, then add water boiling in closed container, in order to the Test paper test vapor that upper arbitrary described rapid identification paddy is newly old, test paper still keeps colourless, shows that rice is fresh, and test paper color is redder shows that the storage time is more of a specified duration.
Preferably, in the method that above-mentioned rapid identification paddy is newly old, described boiling temperature is 100 ~ 110 DEG C, digestion time 5 ~ 15 minutes, and period keeps container closure, and steam does not leak, and container inner pressure controls at 0.02 ~ 0.05MPa.
Preferably, in the method for above-mentioned rapid identification rice new-old, boiling temperature is 105 DEG C, and digestion time is 10 minutes.Because pressure in boiling container is a little more than atmospheric pressure, so keep the temperature of 105 DEG C to be easy to, boiling can make the aldehydes composition major part in rice discharge for 10 minutes, meets testing requirement completely.
Preferably, in the method for above-mentioned rapid identification rice new-old, after boiling terminates, after decrease temperature and pressure, test paper test is carried out again.
Various solution of the present invention, solvent is water.
Compared with prior art, the present invention has following beneficial effect:
Test strips of the present invention is easy to make, and detection reagent sulphuric dioxide used, magenta, mercury oxide and sulfuric acid are all commercially available conventional reagent, and raw material is easy to get with low cost, only needs filter paper impregnation naturally to dry in detection reagent during making.Detection method also only need rice the to add water steam that produces after boiling is fumigated test paper and is observed color change, without the need to by any other instrument, also do not need to be strict with to the technical merit of operating personnel, and application scenarios is almost unrestricted, detection speed is fast, and testing result accuracy rate is high.Using ELISA test strip paddy of the present invention newly old, differentiate that accuracy rate is greater than 90%, is a kind of very reliably and effectively qualitative method determining the new Chen Chengdu of paddy.
Embodiment
In order to make those skilled in the art person understand the present invention program better, below in conjunction with preferred embodiment, the present invention is described in further detail.
The preparation of Test paper:
1) fuchsin solution of 0.01% mass concentration passes into sulfur dioxide gas, until solution becomes colorless;
2) 0.05mol/L mercuric chloride solution and 0.05mol/L sulfuric acid solution is prepared respectively;
3) by colourless fuchsin solution and mercuric chloride solution, sulfuric acid solution is according to for subsequent use as detecting liquid after the volume ratio mixing of 1:1:10;
4) quantitative filter paper to be immersed in the detection liquid of step 3) about 1 minute, then to take out cool place and dry, with scissors cutting into about 5 mm wides, the test paper of 3 centimeter length, low temperature, dry, sealed storage is for subsequent use.
Respectively sample some by fresh paddy (shelf time is less than 1 month) and outmoded paddy (shelf time is about 6 months, 1 year, 2 years), shelling, removes plumule, is prepared into rice sample to be measured some.
Embodiment one:
1) getting the storage time is that rice sample prepared by the paddy of 1 month is about 100g, puts into heatable closed container, adds hot water simultaneously and be about 150g;
2) heat, adjustment temperature is 105 DEG C, cooking rice about 10 minutes, and period keeps container closure, and steam does not leak;
3) lower the temperature, to atmospheric pressure state, with tweezers, test paper is clamped, put into from the aperture that container covers, after about 1 minute, take out, observe the change of test paper color;
5) test paper color is several unchanged, and show that surveyed rice sample is fresh paddy really, testing result shows that this kind of detection method is credible.
Embodiment two:
1) getting the storage time is that rice sample prepared by the paddy of 6 months is about 100g, puts into heatable closed container, adds hot water simultaneously and be about 150g;
2) heat, adjustment temperature is 105 DEG C, cooking rice about 10 minutes, and period keeps container closure, and steam does not leak;
3) lower the temperature, to atmospheric pressure state, with tweezers, test paper is clamped, put into from the aperture that container covers, after about 1 minute, take out, observe the change of test paper color;
5) test paper changes slightly as light red, shows that surveyed rice sample is comparatively fresh, and testing result shows that this kind of detection method is credible.
Embodiment three:
1) getting the storage time is that rice sample prepared by the paddy of 1 year is about 100g, puts into heatable closed container, adds hot water simultaneously and be about 150g;
2) heat, adjustment temperature is 105 DEG C, cooking rice about 10 minutes, and period keeps container closure, and steam does not leak;
3) lower the temperature, to atmospheric pressure state, with tweezers, test paper is clamped, put into from the aperture that container covers, after about 1 minute, take out, observe the change of test paper color;
5) test paper becomes redness, and show that surveyed rice sample is longer for storage time, freshness is not much of that.Testing result shows that this kind of detection method is credible.
Embodiment four:
1) getting the storage time is that rice sample prepared by the paddy of 2 years is about 100g, puts into heatable closed container, adds hot water simultaneously and be about 150g;
2) heat, adjustment temperature is 105 DEG C, cooking rice about 10 minutes, and period keeps container closure, and steam does not leak;
3) lower the temperature, to atmospheric pressure state, with tweezers, test paper is clamped, put into from the aperture that container covers, after about 1 minute, take out, observe the change of test paper color;
5) test paper color transition is peony, shows that surveyed rice sample is very long for storage time, belongs to outmoded paddy.Testing result shows that this kind of detection method is credible.
The explanation of above embodiment just understands core concept of the present invention for helping.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improve and modify and also fall in the protection domain of the claims in the present invention.
Claims (8)
1. the Test paper that rapid identification paddy is newly old, is characterized in that, be test strips dry after immersion detection liquid, described detection liquid contains colourless magenta, mercuric chloride and sulfuric acid; Described colourless magenta passes into SO in fuchsin solution
2form to colourless.
2. the Test paper that rapid identification paddy according to claim 1 is newly old, it is characterized in that, described detection liquid is mixed by colourless fuchsin solution, mercuric chloride solution and the sulfuric acid solution volume ratio according to 1:1:5 ~ 10, wherein pinkish red in colourless fuchsin solution mass concentration is 0.01%, in mercuric chloride solution, the concentration of mercuric chloride is 0.02 ~ 0.08mol/L, and in sulfuric acid solution, the concentration of sulfuric acid is 0.02 ~ 0.08mol/L.
3. the Test paper that rapid identification paddy according to claim 2 is newly old, is characterized in that, in described mercuric chloride solution, the concentration of mercuric chloride is 0.05mol/L, and in sulfuric acid solution, the concentration of sulfuric acid is 0.05mol/L; The volume ratio of colourless fuchsin solution, mercuric chloride solution and sulfuric acid solution is 1:1:10.
4. the Test paper that the rapid identification paddy according to Claims 2 or 3 is newly old, is characterized in that, described test strips is filter paper, and dip time is 1 ~ 2 minute, dries in the cool.
5. the method that a rapid identification paddy is newly old, it is characterized in that, get appropriate paddy processing and become edible rice, then add water boiling in closed container, by the Test paper test vapor that the arbitrary described rapid identification paddy of claim 1 ~ 4 is newly old, test paper still keeps colourless, shows that rice is fresh, and test paper color is redder shows that the storage time is more of a specified duration.
6. the method that rapid identification paddy according to claim 5 is newly old, is characterized in that, described boiling temperature is 100 ~ 110 DEG C, digestion time 5 ~ 15 minutes, and period keeps container closure, and steam does not leak, and container inner pressure controls at 0.02 ~ 0.05MPa.
7. the method that rapid identification paddy according to claim 6 is newly old, is characterized in that, boiling temperature is 105 DEG C, and digestion time is 10 minutes.
8. the method that the rapid identification paddy according to claim 6 or 7 is newly old, is characterized in that, carry out test paper test again after boiling terminates after decrease temperature and pressure.
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| CN201610001843.2A CN105424696B (en) | 2016-01-05 | 2016-01-05 | Rapid identification paddy new old Test paper and detection method |
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| CN201610001843.2A CN105424696B (en) | 2016-01-05 | 2016-01-05 | Rapid identification paddy new old Test paper and detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113049529A (en) * | 2021-03-15 | 2021-06-29 | 湖南省水稻研究所 | Construction method and application of KNN model for detecting rice year |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1641349A (en) * | 2005-01-10 | 2005-07-20 | 江苏省无锡江大大学科技园有限公司 | Reagent for detecting rice new-old degree and its detecting method |
| CN202599849U (en) * | 2012-06-06 | 2012-12-12 | 力德力诺生物技术(北京)有限公司 | Detection pipe used for quickly detecting rice freshness |
| CN103454272A (en) * | 2013-09-13 | 2013-12-18 | 李欣 | Test paper for rapidly judging rice freshness and application of test paper |
| CN104677901A (en) * | 2015-03-31 | 2015-06-03 | 北京普赞生物技术有限公司 | Cereal detection reagent as well as detection method and application thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1641349A (en) * | 2005-01-10 | 2005-07-20 | 江苏省无锡江大大学科技园有限公司 | Reagent for detecting rice new-old degree and its detecting method |
| CN202599849U (en) * | 2012-06-06 | 2012-12-12 | 力德力诺生物技术(北京)有限公司 | Detection pipe used for quickly detecting rice freshness |
| CN103454272A (en) * | 2013-09-13 | 2013-12-18 | 李欣 | Test paper for rapidly judging rice freshness and application of test paper |
| CN104677901A (en) * | 2015-03-31 | 2015-06-03 | 北京普赞生物技术有限公司 | Cereal detection reagent as well as detection method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 童孟良 等: "无机阻燃剂的研究进展", 《化工文摘》 * |
| 鞠洪波: "阻燃材料发展现状与趋势分析", 《绿色科技》 * |
| 魏奉群: "《医学化学》", 30 June 1992 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113049529A (en) * | 2021-03-15 | 2021-06-29 | 湖南省水稻研究所 | Construction method and application of KNN model for detecting rice year |
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