CN105424696B - Rapid identification paddy new old Test paper and detection method - Google Patents
Rapid identification paddy new old Test paper and detection method Download PDFInfo
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- CN105424696B CN105424696B CN201610001843.2A CN201610001843A CN105424696B CN 105424696 B CN105424696 B CN 105424696B CN 201610001843 A CN201610001843 A CN 201610001843A CN 105424696 B CN105424696 B CN 105424696B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N2021/786—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour with auxiliary heating for reaction
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Abstract
The invention discloses a kind of rapid identification paddy new old Test paper and detection method, the new old Test paper of the rapid identification paddy is the test strips dried after immersion detection liquid, and the detection liquid contains colourless magenta, mercury chloride and sulfuric acid；The colourless magenta is that SO is passed through in fuchsin solution2It is formed to colourless.When detection, it is only necessary to rice made of paddy is added to the color change of water-stop boiling then test paper detection steam observation test paper, test paper color still maintains colourless, show paddy be it is fresh, test paper color is redder to show that paddy is older.Method of the invention differentiates that accuracy rate is high, and new old the differentiations accuracy rate of paddy is greater than 90%, and in identification process, method is simple, easy to operate, is one kind very reliably and effectively method of the qualitative new Chen Chengdu of determining paddy.
Description
Technical field
The present invention relates to technical field of food safety detection, the new old Test paper of especially a kind of rapid identification paddy and
Detection method.
Background technique
Rice is one of maximum staple food grain kind in the whole world, and the most important staple food grain kind of China.Its quality quality is straight
Connect the food safety and health for being related to common people.
Rice is most of to need to store realization grain in the form of paddy other than processing for the year, directly feeding consumer
The lasting supply of food, storage time is long up to several years, and short also has some months.The feature of paddy first is that should not store, one
As under storage condition paddy second year begin to Deterioration, some chemical components shown as in rice endosperm change.
Free fatty acid will increase, cell membrane hardens starch composition, the composed structure of the grain of rice changes therewith.
The main distinction of new old rice is the rice cooked, and smell difference is huge, and among these the reason of be exactly aldehyde
The amount of the volatile materials such as class directly affects the flavor of rice.The aldehyde material of the rice of new paddy production during the cooking process
Burst size is low, the distinctive aromatic flavour of rice, and resting period longer old rice, with the extension of assembly time, aldehyde material
(predominantly acetaldehyde, valeral and hexanal) volatile quantity gradually rises, so it is thin out to smell meter fragrance, the peculiar smell taste that even goes mouldy is bright
It is aobvious.
The mouthfeel and nutritive value of old rice are obviously poorer than new rice, and many illegal retailers are in order to improve profit margin, just
New rice will be pretended to be after the old rice polishing waxing of outmoded paddy processing, causes to be difficult to identify that in adoption process and, to obtain non-
Method interests.The inspection of the new Chen Chengdu of paddy is always to perplex the problem of food and beverage enterprise and food enterprise.Based on present market shape
Condition, there is an urgent need to find a kind of new old method of quickly detection rice.
103364515 A(date of publication 2013.10.23 of Chinese patent application publication No. CN) disclose a kind of detection rice
The method of middle aldehyde material, be detected using the method for the derivative solid phase microextraction-gas chromatography combined with mass spectrometry of head space, although
Accuracy is preferable, but needs special installation and environment, and general place is difficult to meet, and detection time is long, detects primary need
Nearly two hours.103454272 A(date of publication 2013.12.18 of Chinese patent application publication No. CN) disclose one kind quickly
The test paper for determining rice new-old degree, is the test strips for being impregnated with the hydrochloric acid-methanol solution of 2,4-dinitrophenylhydrazine.
Summary of the invention
The purpose of the present invention is to provide a kind of Test papers convenient to use, can determine whether rice by observation dye levels
Rice storage time, Detection accuracy is high, and easy to operate the present invention also provides a kind of detection method, application scenarios are virtually limitless
System, detection rates are fast.
The Test paper of rapid identification rice new-old provided by the invention, for the test strips dried after immersion detection liquid, institute
It states detection liquid and contains colourless pinkish red, mercury chloride and sulfuric acid；The colourless magenta is that SO is passed through in fuchsin solution2To colourless
At.
The aldehydes release characteristics of present invention application rice identify the new old property of paddy, and then qualitatively judge paddy or big
The storage time of rice.It is studied by the detection to aldehyde material in rice, finds and be able to detect, distinguish that paddy raw material is newly old
The method of degree, convenient for the newness degree of the rapid identification paddy in adoption process.
Newly old property differentiation is primarily referred to as releasing for aldehyde material (acetaldehyde, valeral, the hexanal) of rice during the cooking process to paddy
High-volume.The aldehyde material burst size of outmoded paddy is larger, by aldehyde material agent for capturing mercury chloride, in the catalytic action of sulfuric acid
Under, oxidation reaction occurs for aldehyde radical and sulfur dioxide, consumes sulfur dioxide, is just restored by the fuchsin solution that sulfur dioxide is bleached before this
At red.Aldehydes quality is more, and the sulfur dioxide for reacting consumption is more, and solution colour is redder.Fresh paddy, due to
Aldehyde material burst size very little will not react sulfur dioxide substantially, therefore fuchsin solution will not revert to red.Rice storage
Period, aldehyde material burst size can change, therefore, can be according to the depth degree (depth of test paper red of magenta color
Shallowly) come qualitatively judge paddy newness degree either storage time.
Preferably, the new old Test paper of above-mentioned rapid identification rice, the detection liquid is by colourless fuchsin solution, mercury chloride
Solution and sulfuric acid solution are mixed according to the volume ratio of 1:1:5 ~ 10, wherein mass concentration pinkish red in colourless fuchsin solution is
0.01%, the concentration of mercury chloride is 0.02 ~ 0.08mol/L in mercuric chloride solution, in sulfuric acid solution the concentration of sulfuric acid be 0.02 ~
0.08mol/L。
Preferably, the new old Test paper of above-mentioned rapid identification rice, the concentration of mercury chloride is in the mercuric chloride solution
0.05mol/L, the concentration of sulfuric acid is 0.05mol/L in sulfuric acid solution；Colourless fuchsin solution, mercuric chloride solution and sulfuric acid solution
Volume ratio is 1:1:10.
Preferably, the new old Test paper of above-mentioned rapid identification rice, the test strips are filter paper, and dip time is 1 ~ 2
Minute, it dries in the cool.It is preferred that dipping 1 minute.
The new old method of a kind of rapid identification paddy provided by the invention, which is characterized in that take appropriate paddy processing at can
Edible rice, then plus water boiling in closed container, with the new old Test paper of the rapid identification paddy of any description above
Test vapor, test paper still keep colourless, show that rice is fresh, and test paper color is redder to show that storage time is more long.
Preferably, in the new old method of above-mentioned rapid identification paddy, the boiling temperature is 100 ~ 110 DEG C, digestion time 5
~ 15 minutes, container closing is during which kept, steam does not leak, and container inner pressure is controlled in 0.02 ~ 0.05MPa.
Preferably, in the method for above-mentioned rapid identification rice new-old, boiling temperature is 105 DEG C, and digestion time is 10 minutes.
Since pressure is slightly above atmospheric pressure in boiling container, so 105 DEG C of temperature is kept to be easy to, rice can be made within boiling 10 minutes
In aldehydes ingredient largely release, fully meet testing requirements.
Preferably, it in the method for above-mentioned rapid identification rice new-old, is tried again after decrease temperature and pressure after boiling
Paper test.
Various solution of the present invention, solvent are water.
Compared with prior art, the invention has the following advantages:
Test strips of the invention are easy to make, and detection reagent sulfur dioxide, magenta, mercury oxide and sulfuric acid used are all
Commercially available conventional reagent, raw material are easy to get low in cost, and when production only needs filter paper impregnation in detection reagent naturally dry i.e.
It can.Detection method also only needs to add rice the steam generated after water boiling to fumigate test paper observation color change, without appointing
What other instruments, does not need to be strict with to the technical level of operator yet, and application scenarios are almost without limitation, detection speed
Degree is fast, and accuracy rate of testing result is high.It is newly old using test strips detection paddy of the invention, differentiate that accuracy rate is greater than 90%, is one
Kind very reliably and effectively method of the qualitative new Chen Chengdu of determining paddy.
Specific embodiment
In order to enable those skilled in the art to better understand the solution of the present invention, below with reference to preferred embodiment
The present invention is described in further detail.
The preparation of Test paper:
1) fuchsin solution of 0.01% mass concentration is passed through sulfur dioxide gas, until solution becomes colorless；
2) 0.05mol/L mercuric chloride solution and 0.05mol/L sulfuric acid solution are prepared respectively；
3) by colourless fuchsin solution and mercuric chloride solution, sulfuric acid solution is used as detection after mixing according to the volume ratio of 1:1:10
Liquid is spare；
4) quantitative filter paper is immersed in the detection liquid of step 3) about 1 minute, then takes out cool place and dry, is cut with scissors
At about 5 mm wides, the test paper of 3 centimeter lengths, low temperature is dry, and sealed storage is spare.
Fresh paddy (storage phase was less than 1 month) and outmoded paddy (storage phase is about 6 months, 1 year, 2 years) are respectively sampled
Several, shelling removes plumule, it is several to be prepared into rice sample to be measured.
Embodiment one:
1) the rice sample about 100g for taking the paddy that storage time is 1 month to prepare, is put into heatable closed container
In, while hot water about 150g is added；
2) it heats, adjustment temperature is 105 DEG C, cooking rice about 10 minutes, during which keeps container closure, steam does not leak；
3) cool down, until normal pressure state, test paper is clamped, be put into from the aperture that container covers with tweezers, about 1 minute
Afterwards, it takes out, observes test paper color change；
5) test paper color is there are few variation, and showing surveyed rice sample really is fresh paddy, and testing result shows this
Kind detection method is credible.
Embodiment two:
1) the rice sample about 100g for taking the paddy that storage time is 6 months to prepare, is put into heatable closed container
In, while hot water about 150g is added；
2) it heats, adjustment temperature is 105 DEG C, cooking rice about 10 minutes, during which keeps container closure, steam does not leak；
3) cool down, until normal pressure state, test paper is clamped, be put into from the aperture that container covers with tweezers, about 1 minute
Afterwards, it takes out, observes test paper color change；
5) test paper slightly changes as light red, shows that surveyed rice sample is more fresh, testing result shows this
Kind detection method is credible.
Embodiment three:
1) the rice sample about 100g for taking the paddy that storage time is 1 year to prepare, is put into heatable closed container
In, while hot water about 150g is added；
2) it heats, adjustment temperature is 105 DEG C, cooking rice about 10 minutes, during which keeps container closure, steam does not leak；
3) cool down, until normal pressure state, test paper is clamped, be put into from the aperture that container covers with tweezers, about 1 minute
Afterwards, it takes out, observes test paper color change；
5) test paper becomes red, shows that surveyed rice sample storage time is longer, freshness is not much of that.Inspection
It surveys the result shows that such detection method is credible.
Example IV:
1) the rice sample about 100g for taking the paddy that storage time is 2 years to prepare, is put into heatable closed container,
Hot water about 150g is added simultaneously；
2) it heats, adjustment temperature is 105 DEG C, cooking rice about 10 minutes, during which keeps container closure, steam does not leak；
3) cool down, until normal pressure state, test paper is clamped, be put into from the aperture that container covers with tweezers, about 1 minute
Afterwards, it takes out, observes test paper color change；
5) test paper color transition is peony, shows that surveyed rice sample storage time is very long, belongs to outmoded rice
Paddy.Testing result shows that such detection method is credible.
The above description of the embodiments is only for helping to understand the core idea of the present invention.It should be pointed out that for this technology
For the those of ordinary skill in field, without departing from the principle of the present invention, several improvement can also be carried out to the present invention
And modification, these improvements and modifications also fall within the scope of protection of the claims of the present invention.
Claims (8)
1. the new old Test paper of rapid identification paddy, which is characterized in that for test strips dry after immersion detection liquid, the inspection
It surveys liquid and contains colourless pinkish red, mercury chloride and sulfuric acid；The colourless magenta is that SO is passed through in fuchsin solution2It is formed to colourless.
2. the new old Test paper of rapid identification paddy according to claim 1, which is characterized in that the detection liquid is by nothing
Color fuchsin solution, mercuric chloride solution and sulfuric acid solution are mixed according to the volume ratio of 1:1:5 ~ 10, wherein colourless fuchsin solution
The mass concentration of middle magenta is 0.01%, and the concentration of mercury chloride is 0.02 ~ 0.08mol/L, sulphur in sulfuric acid solution in mercuric chloride solution
The concentration of acid is 0.02 ~ 0.08mol/L.
3. the new old Test paper of rapid identification paddy according to claim 2, which is characterized in that the mercuric chloride solution
The concentration of middle mercury chloride is 0.05mol/L, and the concentration of sulfuric acid is 0.05mol/L in sulfuric acid solution；Colourless fuchsin solution, mercury chloride
The volume ratio of solution and sulfuric acid solution is 1:1:10.
4. the new old Test paper of rapid identification paddy according to claim 2 or 3, which is characterized in that the test strips
For filter paper, dip time is 1 ~ 2 minute, is dried in the cool.
5. a kind of new old method of rapid identification paddy, which is characterized in that take appropriate paddy processing at edible rice, then plus
Water boiling in closed container, with the new old Test paper test vapor of any rapid identification paddy of claim 1 ~ 4,
Test paper still keeps colourless, shows that rice is fresh, and test paper color is redder to show that storage time is more long.
6. the new old method of rapid identification paddy according to claim 5, which is characterized in that the boiling temperature is 100 ~
110 DEG C, digestion time 5 ~ 15 minutes, during which keep container closing, steam do not leak, container inner pressure control 0.02 ~
0.05MPa。
7. the new old method of rapid identification paddy according to claim 6, which is characterized in that boiling temperature is 105 DEG C, is steamed
Boiling the time is 10 minutes.
8. the new old method of rapid identification paddy according to claim 6 or 7, which is characterized in that pass through after boiling
Test paper test is carried out after decrease temperature and pressure again.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610001843.2A CN105424696B (en) | 2016-01-05 | 2016-01-05 | Rapid identification paddy new old Test paper and detection method |
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| CN201610001843.2A CN105424696B (en) | 2016-01-05 | 2016-01-05 | Rapid identification paddy new old Test paper and detection method |
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| CN105424696B true CN105424696B (en) | 2018-12-28 |
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| CN113049529A (en) * | 2021-03-15 | 2021-06-29 | 湖南省水稻研究所 | Construction method and application of KNN model for detecting rice year |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1641349A (en) * | 2005-01-10 | 2005-07-20 | 江苏省无锡江大大学科技园有限公司 | Reagent for detecting rice new-old degree and its detecting method |
| CN202599849U (en) * | 2012-06-06 | 2012-12-12 | 力德力诺生物技术(北京)有限公司 | Detection pipe used for quickly detecting rice freshness |
| CN103454272A (en) * | 2013-09-13 | 2013-12-18 | 李欣 | Test paper for rapidly judging rice freshness and application of test paper |
| CN104677901A (en) * | 2015-03-31 | 2015-06-03 | 北京普赞生物技术有限公司 | Cereal detection reagent as well as detection method and application thereof |
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2016
- 2016-01-05 CN CN201610001843.2A patent/CN105424696B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1641349A (en) * | 2005-01-10 | 2005-07-20 | 江苏省无锡江大大学科技园有限公司 | Reagent for detecting rice new-old degree and its detecting method |
| CN202599849U (en) * | 2012-06-06 | 2012-12-12 | 力德力诺生物技术(北京)有限公司 | Detection pipe used for quickly detecting rice freshness |
| CN103454272A (en) * | 2013-09-13 | 2013-12-18 | 李欣 | Test paper for rapidly judging rice freshness and application of test paper |
| CN104677901A (en) * | 2015-03-31 | 2015-06-03 | 北京普赞生物技术有限公司 | Cereal detection reagent as well as detection method and application thereof |
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