CN103454272B - A kind of test paper of quick judgement rice new-old degree and application thereof - Google Patents
A kind of test paper of quick judgement rice new-old degree and application thereof Download PDFInfo
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Abstract
The invention discloses a kind of test paper and application thereof of quick judgement rice new-old degree.Described test paper is prepared by the following method and obtains: under room temperature, blank filter paper is immersed 1 ~ 2min in aldehyde material absorbing agent, then takes out, dries, and is cut into setting size, obtains described test paper.Described test paper is as follows for judging the concrete steps of rice new-old degree: Rice Samples and test paper are put into test bottle, with tinfoil, test bottle is sealed bottleneck, then test bottle is heated 10 ~ 60min in 50 ~ 70 DEG C; Take out test paper, read test result, compared with control stripes: if test paper does not develop the color, then Rice Samples is new rice; If test paper displaing yellow, then Rice Samples is old rice.The present invention carries out the detection of rice new-old degree by prepared test paper, simplifies the method for existing absorption rice volatile matter, synchronously carries out chromogenic reaction, simply, effectively, easily implement, can be widely used in rice processing enterprises.
Description
Technical field
The invention belongs to field of detection of food safety, be specifically related to a kind of test paper and application thereof of quick judgement rice new-old degree.
Background technology
Volatile Aldehyde components content in rice (also claiming rice) is the important indicator evaluating White Rice Taste, freshness, is the study hotspot of recent domestic.Fresh rice and the old rice volatile component content after storing a period of time have bigger difference, and as old rice contains more valeral and acetaldehyde than fresh rice, in old rice, the carboxyl compound of 3 ~ 6 carbon atoms accounts for 30% of total odour component, is 10 times of new rice.These carboxyl compounds play ill-effect to rice smell.Rice is along with the prolongation of normal storage fresh keeping time, and food is obviously deteriorated, and color and luster is dimmed, fragrance disappears, acidity increases, and occurs furancarboxylic acid taste and viscosity declines, water absorbing capacity reduces, retention ability goes down, and cook congee the problems such as not thick soup, edible quality reduction.Therefore, for rice processing enterprises, find a kind of reliable, it is most important that easy means screen fresh raw material.
Summary of the invention
For solving the shortcoming and defect part of prior art, primary and foremost purpose of the present invention is the test paper providing a kind of quick judgement rice new-old degree.
Another object of the present invention is to the application process providing above-mentioned test paper, this test paper carries out the judgement of rice new-old degree based on aldehyde material total content in rice, simple to operate, accuracy is high.
For achieving the above object, the present invention adopts following technical scheme:
A test paper for quick judgement rice new-old degree, this test paper is prepared by the following method and obtains: under room temperature, blank filter paper is immersed 1 ~ 2min in aldehyde material absorbing agent, then takes out, dry in the air 25 ~ 35s, be cut into setting size, obtain described test paper.
The of short duration object of drying of 25 ~ 35s keeps test paper macroscopic moistening, not bone dry; Select directly to dry, one is that general baking temperature may not exceed 30 DEG C because aldehyde material absorbing agent is not suitable for baking oven; Two is for convenience of on-the-spot detection.Described blank filter paper is commercially available ordinary filter paper.
Room temperature of the present invention is 15 DEG C ~ 30 DEG C.
Preferably, described aldehyde material absorbing agent is the hydrochloric acid-methanol solution of DNPH.
Wherein methyl alcohol is as solvent; The effect adding hydrochloric acid keeps acid medium.
Preferably, in described aldehyde material absorbing agent, the concentration of DNPH is 5 ~ 50mg/L, and in aldehyde material absorbing agent, the volume fraction of hydrochloric acid is 1.5%.
In the present invention, the concentration of DNPH is 5 ~ 50mg/L, well can control DNPH pickup on test paper at 40 μ g/10cm
2above, thus ensure the sensitivity of test paper.
Preferably, described test paper is strip, is of a size of (2 ~ 5) cm × (5 ~ 8) cm.
Above-mentioned test paper is applied to and judges rice new-old degree; Concrete steps are as follows:
Step one, gets the test paper that two judge rice new-old degree fast, Rice Samples and test paper A is put into test bottle A, test paper B is put into separately test bottle B;
Step 2, seals bottleneck by test bottle A and test bottle B respectively with tinfoil, then two test bottles is heated 10 ~ 60min in 50 ~ 70 DEG C;
Step 3, take out test paper A and test paper B, read test result, compared with test paper B: if test paper A does not develop the color, then Rice Samples is new rice; If test paper A displaing yellow, then Rice Samples is old rice.
New-old degree described in the present invention refers to the freshness of rice, the new-old degree of rice affects by storage condition and storage time, along with storage time extends, the continuous ageing deterioration of rice, Cooking Quality is caused to decline, the inventive method belongs to carries out preliminary screening method to rice new-old degree, is divided into Xin meter and old rice when detecting new-old degree; During detection, if the color displaing yellow of test paper, illustrate that in Rice Samples, aldehyde material total concentration is not less than 20mg/kg, sample is old rice; If when test paper does not develop the color, illustrate that in Rice Samples, aldehyde material total concentration is lower than 20mg/kg, sample is new rice.
The present invention uses tinfoil to seal bottleneck, can avoid interference volatile matter and produce, and tinfoil to be easyly easy to get, can to reuse.
In test process, the test paper object of blank is to eliminate the environmental pollution that may exist in test environment, the test paper of blank does not develop the color under normal circumstances, if there is yellow, then retests after will getting rid of the aldehyde material pollution of operating environment existence.
Preferably, Rice Samples described in step one is 10 ~ 20g.
Above-mentioned preferred sample quality had not only facilitated detection but also can ensure the sensitivity that detects.
Preferably, the mode by water-bath in step 2 heats.
Adopt the mode of water-bath to detect, sample can be made to be heated more even.
Mechanism of the present invention is: containing 2 in test paper of the present invention, 4-dinitrophenylhydrazine, therefore, if when detected rice is old rice, due to can valeral and acetaldehyde be contained in old rice, and aldehydes mass-energy and 2,4-dinitrophenylhydrazine generates yellow phenylhydrazone, test paper can show yellow, by synchronously carrying out chromogenic reaction, thus effectively judges the new-old degree of rice.Further, verified by DNPH column front derivation-high performance liquid chromatography of the prior art, the minimum aldehyde material concentration that test paper of the present invention can detect is 20mg/kg, is 97.2% with above-mentioned chromatographic testing result coincidence rate.
Concrete verification method is as follows: with the standard series of DNPH solution preparation hexanal, makes its abundant reactor vibrates 10s, high effective liquid chromatography for measuring (test condition: UV detect, wavelength 360nm; Mobile phase, acetonitrile+water (volume ratio is 60:40); C18 post), drawing standard curve; Then the test strips after detection is placed in 2mL acetonitrile 1 ~ 2min, measures 360nm light absorption value, aldehyde material total amount in calculation sample.
Compared with prior art, the present invention has the following advantages and beneficial effect:
The present invention's carrier of commercially available filter paper as aldehyde material absorbing agent, prepare a kind of test paper that can detect rice new-old degree, this test paper preparation technology is simple, easy to detect, highly sensitive.
The present invention carries out the detection of rice new-old degree by prepared test paper, simplifies the method for existing absorption rice volatile matter, synchronously carries out chromogenic reaction, simply, effectively, easily implement, can be widely used in rice processing enterprises.
The testing result of test paper of the present invention, contrast with the fatty acid value content detected in Rice Samples according to the method for GB/T20569-2006, coincidence rate is 95.6%, i.e. old rice displaing yellow result, for described in GB/T20569-2006 slightly should not deposit or severe should not be deposited.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
Step one, immerses the hydrochloric acid-methanol solution (2 of DNPH by blank filter paper under room temperature, 4-dinitrophenylhydrazine concentration is 5mg/L, and the volume fraction of hydrochloric acid is 1.5%) middle 2min, then takes out, dries in the air 30s, cut out lower two test paper being of a size of 2cm × 5cm, obtain test paper A and test paper B;
Step 2, takes the 10g Rice Samples (place of production: Guangdong; Conventional stored condition 2 years), Rice Samples and test paper A are put into 150mL triangular flask A, test paper B is put into separately 150mL triangular flask B;
Two triangular flasks, with tinfoil respectively by two triangular flask sealing bottlenecks, are then placed in 50 DEG C of water-baths and heat 10min by step 3;
Step 4, takes out test paper A and test paper B, read test result, compared with test paper B: test paper A is obviously in yellow, and Rice Samples is old rice.
Testing result is verified:
(1) with the standard series of DNPH solution preparation hexanal, make its abundant reactor vibrates 10s, high effective liquid chromatography for measuring (test condition: UV detect, wavelength 360nm; Mobile phase, acetonitrile+water (volume ratio is 60:40); C18 post), drawing standard curve; Then the test strips after detection is placed in 2mL acetonitrile 1 ~ 2min, measure 360nm light absorption value, calculating aldehyde material total amount 56mg/kg in sample, is old rice of the present invention.Illustrate that the present embodiment detection paper result is correct.
(2) detect the fatty acid value content in Rice Samples according to the method for GB/T20569-2006, detect and obtain fatty acid value in Rice Samples: 38 (mg/100g)/(KOH/ dry), for severe should not be deposited.
The present embodiment detection paper result, contrast with the fatty acid value content detected in Rice Samples according to the method for GB/T20569-2006, result is consistent.
Embodiment 2
Step one, immerses the hydrochloric acid-methanol solution (2 of DNPH by blank filter paper under room temperature, 4-dinitrophenylhydrazine concentration is 25mg/L, and the volume fraction of hydrochloric acid is 1.5%) middle 1.5min, then takes out, dries in the air 25s, cut out lower two test paper being of a size of 3cm × 6cm, obtain test paper A and test paper B;
Step 2, takes the 15g Rice Samples (place of production: Guangdong; Conventional stored condition half a year), Rice Samples and test paper A are put into 150mL triangular flask A, test paper B is put into separately 150mL triangular flask B;
Two triangular flasks, with tinfoil respectively by two triangular flask sealing bottlenecks, are then placed in 60 DEG C of water-baths and heat 20min by step 3;
Step 4, take out test paper A and test paper B, read test result, compared with test paper B: test paper A does not obviously develop the color, Rice Samples is new rice.
Testing result is verified:
(1) with the standard series of DNPH solution preparation hexanal, make its abundant reactor vibrates 10s, high effective liquid chromatography for measuring (test condition: UV detect, wavelength 360nm; Mobile phase, acetonitrile+water (volume ratio is 60:40); C18 post), drawing standard curve; Then the test strips after detection is placed in 2mL acetonitrile 1 ~ 2min, measure 360nm light absorption value, calculating aldehyde material total amount 18mg/kg in sample, is new rice of the present invention.Illustrate that the present embodiment detection paper result is correct.
(2) detect the fatty acid value content in Rice Samples according to the method for GB/T20569-2006, detect and obtain fatty acid value in Rice Samples: 22 (mg/100g)/(KOH/ dry), be advisable and deposit.
The present embodiment detection paper result, contrast with the fatty acid value content detected in Rice Samples according to the method for GB/T20569-2006, result is consistent.
Embodiment 3
Step one, under room temperature, blank filter paper is immersed 2, the hydrochloric acid-methanol solution (2 of 4-dinitrophenylhydrazine, 4-dinitrophenylhydrazine concentration is 50mg/L, the volume fraction of hydrochloric acid is 1.5%) middle 1min, then take out, dry in the air 35s, cut out lower 4 test paper being of a size of 4cm × 8cm, obtain test paper A, test paper B, test paper C and test paper D;
Step 2, take rice (place of production: Guangdong) (Rice Samples A) that 20g newly gathers in the crops respectively, 20g deposits rice (the Rice Samples B of 1 year, Hunan, the place of production), 20g deposits rice (the Rice Samples C of 2 years, Jiangxi, the place of production), then Rice Samples A and test paper A is put into 150mL triangular flask A, Rice Samples B and test paper B is put into 150mL triangular flask B, Rice Samples C and test paper C is put into 150mL triangular flask C, test paper D is put into separately 150mL triangular flask D;
4 triangular flasks, with tinfoil respectively by 4 triangular flask sealing bottlenecks, are then placed in 70 DEG C of water-baths and heat 60min by step 3;
Step 4, take out test paper A, test paper B, test paper C and test paper D, read test result, compared with test paper D: test paper A does not develop the color, test paper B displaing yellow, test paper C shows buff.
Testing result is verified:
(1) with the standard series of DNPH solution preparation hexanal, make its abundant reactor vibrates 10s, high effective liquid chromatography for measuring (test condition: UV detect, wavelength 360nm; Mobile phase, acetonitrile+water (volume ratio is 60:40); C18 post), drawing standard curve; Then test paper A, the test paper B after detection, test paper C are placed in 2mL acetonitrile 1 ~ 2min, measure 360nm light absorption value, calculate aldehyde material total amount in sample A, B, C and be respectively 8mg/kg, 23mg/kg and 61mg/kg, therefore Rice Samples A is new rice of the present invention, Rice Samples B is old rice of the present invention, and Rice Samples C is old rice of the present invention.Illustrate that the present embodiment detection paper result is correct.
(2) detect the fatty acid value content in above-mentioned Rice Samples according to the method for GB/T20569-2006, detect and obtain fatty acid value in Rice Samples A: 16 (mg/100g)/(KOH/ dry), be advisable and deposit, conform to detection paper result; Fatty acid value in Rice Samples B: 27 (mg/100g)/(KOH/ dry), is advisable and deposits; Fatty acid value in Rice Samples C: 38 (mg/100g)/(KOH/ dry), for severe should not be deposited, conforms to detection paper result.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (3)
1. judge an application for the test paper of rice new-old degree fast, it is characterized in that, described test paper is as follows for judging the concrete steps of rice new-old degree:
Step one, gets two test paper, Rice Samples and test paper A is put into test bottle A, test paper B is put into separately test bottle B;
Step 2, seals bottleneck by test bottle A and test bottle B respectively with tinfoil, then two test bottles is heated 10 ~ 60min in 50 ~ 70 DEG C;
Step 3, take out test paper A and test paper B, read test result, compared with test paper B: if test paper A does not develop the color, then Rice Samples is new rice; If test paper A displaing yellow, then Rice Samples is old rice;
Described test paper is prepared by the following method and obtains: under room temperature, blank filter paper is immersed 1 ~ 2min in aldehyde material absorbing agent, then takes out, dry in the air 25 ~ 35s, be cut into setting size, obtain described test paper;
Described aldehyde material absorbing agent is the hydrochloric acid-methanol solution of DNPH;
In described aldehyde material absorbing agent, the concentration of DNPH is 5 ~ 50mg/L, and in aldehyde material absorbing agent, the volume fraction of hydrochloric acid is 1.5%.
2. test paper according to claim 1 is judging the application in rice new-old degree, and it is characterized in that, Rice Samples described in step one is 10 ~ 20g.
3. test paper according to claim 1 is judging the application in rice new-old degree, and it is characterized in that, the mode by water-bath in step 2 heats.
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| CN105424696B (en) * | 2016-01-05 | 2018-12-28 | 北京北大明德科技发展有限公司 | Rapid identification paddy new old Test paper and detection method |
| CN111289508A (en) * | 2018-12-07 | 2020-06-16 | 云南商测质量检验技术服务有限公司 | Acid value rapid test paper and detection method thereof |
| CN112921505A (en) * | 2021-02-24 | 2021-06-08 | 福州大学 | Preparation method of electrospinning blotting chromogenic membrane with high efficiency and selectivity on hexanal |
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| US20060105464A1 (en) * | 2004-11-12 | 2006-05-18 | Weber Jorg Friedrich W | Quantitative determination of analyte |
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| CN202486057U (en) * | 2011-12-28 | 2012-10-10 | 邯郸派瑞电器有限公司 | Test paper for fast formaldehyde detection |
| CN102895837A (en) * | 2012-02-13 | 2013-01-30 | 中国人民解放军海军医学研究所 | Composition used for eliminating compounds of aldehydes and ketones |
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