CN102944550A - Detection kit for calcium peroxide in flour and detection method for calcium peroxide - Google Patents
Detection kit for calcium peroxide in flour and detection method for calcium peroxide Download PDFInfo
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- CN102944550A CN102944550A CN2012103888326A CN201210388832A CN102944550A CN 102944550 A CN102944550 A CN 102944550A CN 2012103888326 A CN2012103888326 A CN 2012103888326A CN 201210388832 A CN201210388832 A CN 201210388832A CN 102944550 A CN102944550 A CN 102944550A
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- calper calcium
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Abstract
The present invention provides a detection kit for calcium peroxide in flour, which is composed of plastic sample spoons, plastic droppers, test tubes, a measuring cylinder, a test tube rack, a standard colorimetric card, a chromogenic reagent, pH test strips, reaction reagents, and a wash bottle. Tests are carried out on the samples by observing the different colors being shown and contrasting with the standard colorimetric card to perform qualitative and semi-quantitative detections. The detection method for calcium peroxide in flour includes: adding dilute acid into flour to react, adjusting the pH value to neutral with an alkaline solution, adding a mixed solution including a peroxidase-containing reagent, developing color, comparing with the standard colorimetric card to obtain the qualitative and semi-quantitative result. The detection kit and the detection method of the invention have the advantages of easy usage, fast color developing, relatively loose detection conditions, high sensitivity and low cost, and are suitable for on-site rapid screening assays.
Description
Technical field
The present invention relates to detect with kit and application thereof, especially relate to the kit of calper calcium peroxide in a kind of Fast Measurement flour and to the assay method of artificial admixture calper calcium peroxide quantitative and semi-quantitative in the flour.
Background technology
Calper calcium peroxide is light yellow crystalline powder, and molecular weight 72.08 is widely used in the industries such as food, chemical industry, weaving.The fields such as after-ripening, the flour that is usually used in improving wheat flour brightens, flour gluten fortifier, flour decolorant, the bleaching of bread improver edible oil.For chewiness and the mouthfeel that increases wheat flour, the flour industry generally adds benzoyl peroxide, calper calcium peroxide as wheat flour gluten fortifier, whitening agent.China forbade adding benzoyl peroxide, calper calcium peroxide [seven (office) issues " about cancelling the bulletin of food additives benzoyl peroxide, calper calcium peroxide " (2011 No. 4) such as the Ministry of Public Health] in Flour production from 1 day May in 2011.
Calper calcium peroxide is added in the flour, and long-term eating may be carcinogenic, can produce very large harm to health.And some Flour production producer may add excessively benzoyl peroxide or calper calcium peroxide in violation of rules and regulations for the slaking of flour decolouring, quickening flour.In existing national standard, industry standard and the open source literature, also do not detect method and the quick measuring reagent box of calper calcium peroxide in the flour.Therefore, set up one fast and accurately in the flour calper calcium peroxide qualitative and the semiquantitative determination method is significant.
Summary of the invention
The object of the invention provides a kind of kit to calper calcium peroxide fast detecting in the flour, and uses this kit carries out quantitative and semi-quantitative to calper calcium peroxide in the flour assay method.For this reason, the present invention adopts following technical scheme:
The calper calcium peroxide quick measuring reagent box is characterized in that in the flour
(1) plastic sample spoon is filled approximately 2g of one flat spoonful of flour quality;
(2) be with graduated disposable plastic dropper 3mL;
(3) 50mL tool plug glass color comparison tube;
(4) 50mL glass or plastic cylinder;
(5) test tube rack, the diameter in hole is fit to 50mL tool plug glass color comparison tube;
(6) standard color comparison card, standard color comparison card are the serial colour atla that pale red to peony changes in gradient, the content of the calper calcium peroxide of all corresponding its representative below every;
(7) peroxidase chromogenic reagent;
(8) wide pH value test paper (1-14) and accurate pH test paper (6.4-8.0);
(9) reaction reagent: the reagent of three plastic bottles, every bottle amasss 500-1000mL, is respectively 1% dilute sulfuric acid, 20% dilute sodium hydroxide, 0.1% NaOH;
(10) 500mL plastic squeeze bottle.
The application of calper calcium peroxide quick detection kit in the flour, its concrete steps are:
(1) get one spoonful of sample, add 1% dilute sulfuric acid 20mL, vortex mixed is even, fully reacts 10min;
(2) add 20% sodium hydroxide solution and regulate pH between the 5-6, then with 0.1% sodium hydroxide solution regulator solution pH value to 7.0 with the wide pH value test paper.
(3) add peroxidase chromogenic reagent solution 2mL, constant volume behind the colour developing 5-10min, is observed color to 50mL under the room temperature.
(4) with gained color and standard color comparison card contrast.
Step (3) peroxidase reagent mix solution is made of matching while using composite reagent.The A bottle contains horseradish peroxidase 2000U (unit of activity); The B bottle contains phosphate buffer (pH=7.6) 200mL of the 0.2mol/L of 4-AA 0.00154mol/L; The C bottle contains 0.022mol/L phenol solution 200mL.During use, first B and C solution are fully mixed, then the enzyme among the A is dissolved in the mixed liquor, shake gently, enzyme is dissolved fully, be the peroxidase reagent solution, enzyme activity 10U (unit of activity)/mL.
The result judges, if the sample solution nondiscolouring does not then have the admixture calper calcium peroxide, belongs to qualified flour; If solution has change color, then for being mixed with calper calcium peroxide.Although benzoyl peroxide also is flour processing whitening agent commonly used, belongs to strong oxidizer, under the condition of the method, do not affect testing result containing under benzoyl peroxide 1000mg/kg and the following content condition.
Peroxide is crossed the method for making of calcium examination criteria colorimetric card in the flour:
(1) accurately takes by weighing 10g and do not contain the flour of calper calcium peroxide as blank matrix, the calper calcium peroxide standard items that add accurate weighing are mixed with the serial mark-on content of calper calcium peroxide, and adding respectively the 50mL mass concentration is 1% dilute sulfuric acid, the vortex mixing fully reacts 10min.
(2) adjust pH value to 7.0 with 20% and 0.1% sodium hydroxide solution.
(3) add peroxidase composite reagent solution, constant temperature colour developing 20min is cooled to room temperature in 37 ℃ of water-baths, is settled to 50mL.
(4) standard solution with colour developing gathers photo, and one group of photochrome of graded is made standard color comparison card.The depth according to color is come quantitative and semi-quantitative.
The blank matrix mark-on concentration gradients scope of preparing in the method for making (1) is preferably between 20mg/kg-1000mg/kg, such as 20mg/kg, 50mg/kg, 100mg/kg, 200mg/kg, 500mg/kg, 1000mg/kg.
Beneficial effect:
1) method of calper calcium peroxide in kit method fast qualitative and the half-quantitative detection flour, method is simple, quick, highly sensitive, the result is distinct.
2) utilize the detection kit of calper calcium peroxide in the flour of the present invention, be convenient to estimate qualitative and on-the-spot rapid screening and measure.
3) detection kit and the detection method thereof of calper calcium peroxide in the flour of the present invention do not need other annex instruments, and easy operating is practical.
Description of drawings
Calper calcium peroxide standard color comparison card in Fig. 1 flour
Embodiment
The below is described in further details the present invention.
Embodiment 1:
The preparation of standard color comparison card
Analytical procedure: the wheat flour sample 10g that accurate weighing does not contain the calper calcium peroxide whitening agent (is accurate to 0.01g in the 100mL color comparison tube, and then take by weighing respectively high purity reagent calper calcium peroxide 0mg, 0.20mg, 0.50mg, 1.00mg, 2.00mg, 5.00mg, 10.00mg and join in the color comparison tube, and add successively 1% dilute sulfuric acid 50mL, the vortex mixing at room temperature reacts 10min.Sodium hydroxide solution with 20% and 0.1% is adjusted pH value to 7.0, add 3mL enzyme reagent solution, with the intermediate water constant volume to 100mL, constant temperature 10min in 37 ℃ of water-baths of horizontal shaking table, be cooled to room temperature, namely get serial blank matrix standard solution, corresponding per kilogram flour contains calper calcium peroxide 0mg, 20mg, 50mg, 100mg, 200mg, 500mg, 1000mg respectively.Take a picture for the solution of colour developing, gather color and be made into standard color comparison card (seeing Fig. 1).
Embodiment 2:
The calper calcium peroxide quick measuring reagent box
Kit is equipped with: the plastic sample spoon, 2, fill approximately 2g of one flat spoonful of flour quality; 3mL is with graduated disposable plastic dropper, 100; 50mL tool plug glass color comparison tube, 30; The 50mL plastic cylinder, 1; Test tube rack, 1,20 holes, the diameter in hole is fit to 50mL tool plug glass color comparison tube; Standard color comparison card, 1, for containing blank and pale red to 6 serial vittas that change in gradient of peony, the content of the calper calcium peroxide of its representative of mark below every; Wide pH value test paper (1-14), 2 boxes; Accurate pH test paper (6.4-8.0), 2 boxes; Reaction reagent: the reagent of three kinds of plastic bottles is denoted as 1% dilute sulfuric acid, 20% dilute sodium hydroxide, 0.1% NaOH, every bottled 500mL.The 500mL plastic squeeze bottle, in-built distilled water, 1.
Embodiment 3:
Analytical procedure: get one spoonful of certain brand dumpling flour in the 50mL color comparison tube, add 1% dilute sulfuric acid 20mL, at room temperature react 10min, after being cooled to room temperature, adjust pH value to 7.0 with 20% and 0.1% sodium hydroxide solution, add 2mL enzyme reagent solution, be settled to scale with intermediate water, shake up.Behind the colour developing 5min, observe color under the room temperature.Sample is peach turbid solution, illustrates in this sample to contain calper calcium peroxide.Through contrasting with standard color comparison card, the standard vitta color of test solution color and 100mg/kg approaches, and illustrates that calper calcium peroxide detects in this sample, and content is about 100mg/kg.
Above-described embodiment is only for explanation the present invention, and is not to be restriction to patent of the present invention; Should be pointed out that for those of ordinary skill in the art, conceive scope in the situation that do not break away from the present invention, can also make various variations and modification, these all belong to protection scope of the present invention; Therefore, all equalizations of doing with claim scope of the present invention change and modify, and all should belong to the coverage of claim of the present invention.
Claims (3)
1. calper calcium peroxide quick measuring reagent box in the flour is characterized in that, the box that is comprised of plastic sample spoon, plastic dropper, test tube, graduated cylinder, test tube rack, standard color comparison card, peroxidase chromogenic reagent, pH test paper, reaction reagent is housed; Wherein reaction reagent is 1% dilute sulfuric acid, 20% dilute sodium hydroxide, 0.1% NaOH, and standard color comparison card is the serial colour atla that pale red to peony changes in gradient.
2. method according to claim 1, it is characterized in that, the pink of standard color comparison card is for being divided into from shallow to deep 6-8 class, and be 20mg/kg to 1000mg/kg to the calper calcium peroxide concentration of the corresponding mark of different colours representative, such as 20mg/kg, 50mg/kg, 100mg/kg, 200mg/kg, 500mg/kg, 1000mg/kg.
3. the application of calper calcium peroxide quick measuring reagent box in the flour is characterized in that, gets one flat spoonful of sample, adds 1% dilute sulfuric acid 20mL, and vortex mixed is even, fully reacts 10min; Regulate pH value to 7.0 with sodium hydroxide solution; Add peroxidase chromogenic reagent 2mL, constant volume, the 5-10min that develops the color under the room temperature with gained color and standard color comparison card contrast, determines the content range of calper calcium peroxide in the flour.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103424402A (en) * | 2013-07-02 | 2013-12-04 | 福建省明溪海天蓝波生物技术有限公司 | Body fluid sulfydryl detection kit |
| CN103983643A (en) * | 2014-06-03 | 2014-08-13 | 河南工业大学 | Kit for rapidly detecting calcium peroxide in wheat flour and detecting method of kit |
| CN103983591A (en) * | 2014-06-03 | 2014-08-13 | 河南工业大学 | Method for rapidly detecting content of calcium peroxide in wheat flour |
| CN106596526A (en) * | 2015-10-20 | 2017-04-26 | 谱尼测试集团江苏有限公司 | Detection method for calcium peroxide content in flour |
| CN109444121A (en) * | 2018-10-16 | 2019-03-08 | 河南工业大学 | The detection kit and detection method of calper calcium peroxide in a kind of flour |
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| US4478942A (en) * | 1981-02-27 | 1984-10-23 | Fuji Photo Film Co., Ltd. | Quantitative analysis film and a method for colorimetric analysis using the same |
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| CN101034065A (en) * | 2006-03-07 | 2007-09-12 | 桑华春 | Reagent kit for quickly testing protein content of milk powder and method for making same |
| CN101393158A (en) * | 2008-10-23 | 2009-03-25 | 上海交通大学 | Biology sensing enzyme detection electrode for hydrogen peroxide and detection method thereof |
| CN101639445A (en) * | 2009-09-07 | 2010-02-03 | 福建省洪诚生物药业有限公司 | Method for detecting blood pyruvate in vitro by using chemiluminescence method |
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Patent Citations (7)
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| US4251629A (en) * | 1977-07-29 | 1981-02-17 | Wako Pure Chemical Industries, Ltd. | Determination of hydrogen peroxide |
| US4478942A (en) * | 1981-02-27 | 1984-10-23 | Fuji Photo Film Co., Ltd. | Quantitative analysis film and a method for colorimetric analysis using the same |
| CN1502990A (en) * | 2002-10-31 | 2004-06-09 | 世诺临床诊断制品株式会社 | Reagent for testing calcium in sample and test method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103424402A (en) * | 2013-07-02 | 2013-12-04 | 福建省明溪海天蓝波生物技术有限公司 | Body fluid sulfydryl detection kit |
| CN103424402B (en) * | 2013-07-02 | 2017-03-29 | 福建省明溪海天蓝波生物技术有限公司 | A kind of body fluid sulfydryl detection kit |
| CN103983643A (en) * | 2014-06-03 | 2014-08-13 | 河南工业大学 | Kit for rapidly detecting calcium peroxide in wheat flour and detecting method of kit |
| CN103983591A (en) * | 2014-06-03 | 2014-08-13 | 河南工业大学 | Method for rapidly detecting content of calcium peroxide in wheat flour |
| CN106596526A (en) * | 2015-10-20 | 2017-04-26 | 谱尼测试集团江苏有限公司 | Detection method for calcium peroxide content in flour |
| CN109444121A (en) * | 2018-10-16 | 2019-03-08 | 河南工业大学 | The detection kit and detection method of calper calcium peroxide in a kind of flour |
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| CN102944550B (en) | 2015-07-15 |
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Address after: 201613 2nd Floor, Building 7, 99 Wenxiang East Road, Songjiang District, Shanghai Patentee after: PONY TESTING INTERNATIONAL GROUP SHANGHAI CO., LTD. Address before: 2004-233 4th Floor, Building 35, 680 Guiping Road, Xuhui District, Shanghai Patentee before: Shanghai Pony Testing Technology Co., Ltd. |