CN103424402A - Body fluid sulfydryl detection kit - Google Patents
Body fluid sulfydryl detection kit Download PDFInfo
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- CN103424402A CN103424402A CN2013102743112A CN201310274311A CN103424402A CN 103424402 A CN103424402 A CN 103424402A CN 2013102743112 A CN2013102743112 A CN 2013102743112A CN 201310274311 A CN201310274311 A CN 201310274311A CN 103424402 A CN103424402 A CN 103424402A
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Abstract
The invention discloses a body fluid sulfydryl detection kit which comprises phosphotungstic acid, first buffer solution, mercuric chloride, first test paper and second test paper, wherein test paper becomes the first test paper after being soaked in phosphotungstic acid solution and then dried, other test paper becomes the second test paper after being soaked in mercuric chloride and the first buffer solution and then dried, the first test paper and the second test paper are adhered or close to each other in other ways to form compound test paper; body fluid is dripped on the first test paper, and the body fluid and reagents on the first test paper penetrate into the second test paper to detect the sulfydryl of the body fluid. All reagents components are compounded during the body fluid sulfydryl detection process, unstable chemical reagents separate the first buffer solution from phosphotungstic acid, which interact, through drying stabilization and mechanical structure design, so that the stability of the reagents at the normal temperature is improved greatly, any auxiliary reagents and devices are not required during the detection process, the speed is high, the operation is simple, and the large-scale population screening operation can be performed quickly.
Description
Technical field
The invention belongs to rapid in-vitro diagnostic kit field, be specifically related to a kind of body fluid sulfydryl detection kit.
Background technology
The cervical cancer pathogenesis rate occupies first of women's cancer, at present, the method of the diagnosis and detection for cervix cancer in clinical practice has: the inspection of cervix Scrape method, iodine test, uterine neck and cervical canal biopsy, vaginoscopy, conization, TCT detect, high-risk HPV detects etc., but it is the shortcomings such as testing process professional technique conditional request complicated, that detect is high, the time is long, expense is high that these detection methods and technology all exist, can only detect and use in specialist hospital, be difficult to extensively promote in the crowd and use." the portable long-acting fast urine detection of SH kit " of the people such as Lin Genchun invention is although be quick, an easy method, but kit still exists and forms the deficiencies such as structure is more complicated, operation steps is many, and the reagent holding time in the mentioned reagent box is short, poor stability.
Summary of the invention
The object of the invention is to overcome the prior art defect, a kind of body fluid sulfydryl detection kit is provided.
Technical scheme of the present invention is as follows:
A kind of body fluid sulfydryl detection kit, comprise phosphotungstic acid, the first damping fluid and mercuric chloride and first, second test paper, test paper becomes the first test paper after soaking the Salkowski's solution drying, another test paper is the second test paper after soaking mercuric chloride and the first damping fluid drying, first, second test paper laminating or alternate manner is mutual to forming compound test paper, body fluid is dripped on the first test paper, and the reagent of body fluid and the first test paper penetrates on the second test paper, detects the sulfydryl of body fluid.
In a preferred embodiment of the invention, described compound test paper comprises the first compound test paper, the described first compound test paper comprises one first connected epimere test paper and one first hypomere test paper, described the first epimere test paper is made after soaking the first damping fluid drying, and described the first hypomere test paper is made after soaking phosphotungstic acid and the second damping fluid drying.
Preferably, described compound test paper also comprises the second compound test paper, the described second compound test paper paper bag is drawn together one second connected epimere test paper and one second hypomere test paper, described the second epimere test paper is made after soaking the first damping fluid and mercuric chloride drying, and described the second hypomere test paper is made after soaking phosphotungstic acid, mercuric chloride and the second damping fluid drying.
Preferably, also comprise a panel and a base plate, described base plate is provided with the first groove and the second groove of installing respectively the first compound test paper and the second compound test paper, on described panel, corresponding the first groove offers the first well and the first colour developing hole, and corresponding the second groove is provided with the second well and the second colour developing hole.
Preferably, described the first well below is the first epimere test paper, and described the first below, colour developing hole is the first hypomere test paper; Described the second well below is the second epimere test paper, and described the second below, colour developing hole is the second hypomere test paper.
Preferably, between described the first epimere test paper and the first hypomere test paper, also establish a compartmented; Yet establish a compartmented between described the second epimere paper and described the second hypomere paper.
In a preferred embodiment of the invention, described compound test paper comprises the first compound test paper, the described first compound test paper comprises the first upper strata test paper and the first lower floor's test paper fit together, described the first upper strata test paper is made after soaking the first damping fluid drying, and described first lower floor's test paper is made after soaking phosphotungstic acid and the second damping fluid drying.
Preferably, described compound test paper also comprises the second compound test paper, the described second compound test paper comprises the second upper strata test paper and the second lower floor's test paper fit together, described the second upper strata test paper is made after soaking the first damping fluid and mercuric chloride drying, and described second lower floor's test paper is made after soaking phosphotungstic acid, mercuric chloride and the second damping fluid drying.
Preferably, also comprise a panel and a base plate, offer the first colour developing hole and the second colour developing hole on base plate, on panel, corresponding the first colour developing hole and the second colour developing hole offer respectively the first well and the second well, the first compound test paper is located between the first colour developing hole and the first well, and the second compound test paper is located between the second colour developing hole and the second well.
Preferably, described the first well below is the first upper strata test paper, and the below of described first lower floor's test paper is the first colour developing hole; Described the second well below is the second upper strata test paper, and the below of described second lower floor's test paper is the second colour developing hole.
Preferably, between described the first upper strata test paper and first lower floor's test paper, also establish a separate layer; Yet establish a separate layer between described the second upper paper and described second lower floor's paper.
Another technical scheme of the present invention is as follows:
A kind of body fluid detection kit, comprise the first reagent, the second reagent and first, second test paper, test paper becomes the first test paper after soaking the first reagent drying, another test paper is the second test paper after soaking the second reagent drying, first, second test paper laminating or alternate manner is mutual to forming compound test paper, body fluid is splashed on the first test paper, and the reagent of body fluid and the first test paper penetrates on the second test paper, detects the material of body fluid.
In a preferred embodiment of the invention, described the first reagent, the second reagent are single dose or are complex reagent.
A technical scheme more of the present invention is as follows:
A kind of body fluid detection kit, comprise the first reagent, the second reagent and first, second adsorbed layer, adsorbed layer becomes the first adsorbed layer after soaking the first reagent drying, another adsorbed layer is the second adsorbed layer after soaking the second reagent drying, first, second adsorbed layer laminating or alternate manner is mutual to forming the composite adsorption layer, body fluid is splashed on the first adsorbed layer, and the reagent on body fluid and the first adsorbed layer penetrates on the second adsorbed layer, detects the material of body fluid.
The invention has the beneficial effects as follows:
1, the present invention is compounded with all reagent compositions of body fluid sulfydryl testing process, unsettled chemical reagent is stablized and separated the first damping fluid and the phosphotungstic acid that can react to each other with Design of Mechanical Structure by mummification, reagent stability is at normal temperatures improved greatly, checking process is without any auxiliary reagent and equipment, only need to splash into respectively a body fluid sample at the first well and the second well, after 10 minutes, observations gets final product, speed is fast, simple to operate, can carry out fast large-scale Mass screening work;
2, of the present invention simple in structure, with low cost, be applicable to suitability for industrialized production.
The accompanying drawing explanation
The structural representation of the panel that Fig. 1 is embodiments of the invention 1
The structural representation of the base plate that Fig. 2 is embodiments of the invention 1;
The front elevation of the first compound test paper that Fig. 3 is embodiments of the invention 1;
Fig. 4 is the cut-open view of Fig. 3 along the A-A line;
The front elevation of the second compound test paper that Fig. 5 is embodiments of the invention 1;
Fig. 6 is the cut-open view of Fig. 5 along the B-B line.
The structural representation of the panel that Fig. 7 is embodiments of the invention 2
The structural representation of the base plate that Fig. 8 is embodiments of the invention 2;
The front elevation of the first compound test paper that Fig. 9 is embodiments of the invention 2;
Figure 10 is the cut-open view of Fig. 9 along the C-C line;
The front elevation of the second compound test paper that Figure 11 is embodiments of the invention 2;
Figure 12 is the cut-open view of Figure 11 along the D-D line.
Embodiment
Below by embodiment in connection with accompanying drawing, technical scheme of the present invention is further detailed and describes.
As depicted in figs. 1 and 2, a kind of body fluid sulfydryl detection kit, a panel 3 and a base plate 4 together with comprising one first compound test paper 1, one second compound test paper 2 and being installed in up and down by buckle, base plate 4 is provided with the first groove 43 and the second groove 44 of installing respectively the first compound test paper 1 and the second compound test paper 2, on described panel 3, corresponding the first groove 43 offers the first well 31 and the first colour developing hole 41, and corresponding the second groove 44 is provided with the second well 32 and the second colour developing hole 42; Described panel forms kit together with being installed in up and down by bonding or buckle with base plate, or described panel side embeds base plate generate a reagent box.
As shown in Figure 3 and Figure 4, the described first compound test paper 1 comprises the hard sheet 13 of one first paper, the hard sheet 13 of this first paper is provided with the first hypomere test paper 12 that soaks dry the first epimere test paper 11 formed and form with phosphotungstic acid, the second damping fluid immersion drying with the first damping fluid partly overlapped together, preferably between the first epimere test paper and the first hypomere test paper, also establishes a compartmented; Described the first well 31 belows are the first epimere test paper 11, and described the first colour developing 41 belows, hole are the first hypomere test paper 12.
As shown in Figure 5 and Figure 6, the described second compound test paper 2 comprises the hard sheet 23 of one second paper, the hard sheet 23 of this second paper is provided with the second hypomere test paper 23 that soaks dry the second epimere test paper 21 formed and form with phosphotungstic acid, the second damping fluid and mercuric chloride immersion drying with the first damping fluid, mercuric chloride partly overlapped together, preferably between the second epimere test paper and the second hypomere test paper, also establishes a compartmented; Described the second well 32 belows are the second epimere test paper 21, and described the second colour developing 42 belows, hole are the second hypomere test paper 22.
Using method of the present invention is as follows:
Splash into respectively women's body fluid sample to be detected at the first well 31 and the second well 32, observe the blueness reaction in the first colour developing hole 41 and the second colour developing hole 42 after 10 minutes, when the blue depth in the first colour developing hole 41 and the second colour developing hole 42 when consistent, negative result, show not have evidence explanation detected person to suffer from cervix cancer or body precancerous lesion (CIN) occur; When the blueness in the first colour developing hole 41 is darker than the blueness in the second colour developing hole 42, positive result, show that the detected person may suffer from generation precancerous lesion (CIN) or the rear disease relapse for the treatment of in cervix cancer, body, can advise that the detected person receives further inspection to make a definite diagnosis.
As shown in Figure 7 and Figure 8, a kind of body fluid sulfydryl detection kit, a panel 3 and a base plate 4 together with comprising one first compound test paper 1, one second compound test paper 2 and being installed in up and down, offer the first colour developing hole 41 and the second colour developing hole 42 on base plate 4, on panel 3, corresponding the first colour developing hole 41 and the second colour developing hole 42 offer respectively the first well 31 and the second well 32, the first compound test paper 1 is pasted and is connected between the first colour developing hole 41 and the first well 31, and the second compound test paper 2 is pasted and is connected between the second colour developing hole 42 and the second well 32; Described panel, the bonding kit that forms of base plate, or described panel side embeds base plate generate a reagent box.
As shown in Figure 9 and Figure 10, the described first compound test paper 1 comprises overlapping with the first damping fluid, soaking dry the first upper strata test paper 11 formed and soak the dry first lower floor's test paper 12 formed with phosphotungstic acid and the second damping fluid of fitting together, described the first well below is the first upper strata test paper, and the below of described first lower floor's test paper is the first colour developing hole; Preferably, also be provided with a separate layer between described the first upper strata test paper and first lower floor's test paper.
As shown in Figure 11 and Figure 12, the described second compound test paper 2 comprises overlapping with the first damping fluid, mercuric chloride, soaking dry the second upper strata test paper 21 formed and soak the dry second lower floor's test paper 22 formed with phosphotungstic acid, the second damping fluid and mercuric chloride of fitting together, described the second well below is the second upper strata test paper, and the below of described second lower floor's test paper is the second colour developing hole; Preferably, also be provided with a separate layer between described the second upper strata test paper and second lower floor's test paper.
The using method of this kit is as follows:
Splash into respectively a female urine fluid samples to be detected at the first well 31 and the second well 32, observe the blueness reaction in the first colour developing hole 41 and the second colour developing hole 42 after 10 minutes, when the blue depth in the first colour developing hole 41 and the second colour developing hole 42 when consistent, negative result, show not have evidence explanation detected person to suffer from cervix cancer or body precancerous lesion (CIN) occur; When the blueness in the first colour developing hole 41 is darker than the blueness in the second colour developing hole 42, positive result, show that the detected person may suffer from generation precancerous lesion (CIN) or the rear disease relapse for the treatment of in cervix cancer, body, can advise that the detected person receives further inspection to make a definite diagnosis.
Embodiment 3
A kind of body fluid detection kit, comprise the first reagent, the second reagent and first, second test paper, test paper becomes the first test paper after soaking the first reagent drying, another test paper is the second test paper after soaking the second reagent drying, first, second test paper laminating or alternate manner is mutual to forming compound test paper, body fluid is splashed on the first test paper, and the reagent of body fluid and the first test paper penetrates on the second test paper, detects the material of body fluid.Wherein, described the first reagent, the second reagent are single dose or are complex reagent.
A kind of body fluid detection kit, comprise the first reagent, the second reagent and first, second adsorbed layer, adsorbed layer becomes the first adsorbed layer after soaking the first reagent drying, another adsorbed layer is the second adsorbed layer after soaking the second reagent drying, first, second adsorbed layer laminating or alternate manner is mutual to forming the composite adsorption layer, body fluid is splashed on the first adsorbed layer, and the reagent on body fluid and the first adsorbed layer penetrates on the second adsorbed layer, detects the material of body fluid.
The above, be only preferred embodiment of the present invention, therefore can not limit according to this scope of the invention process, the equivalence done according to the scope of the claims of the present invention and description changes and modifies, and all should still belong in the scope that the present invention contains.
Claims (14)
1. a body fluid sulfydryl detection kit, comprise phosphotungstic acid, the first damping fluid and mercuric chloride and first, second test paper, it is characterized in that: test paper becomes the first test paper after soaking the Salkowski's solution drying, another test paper is the second test paper after soaking mercuric chloride and the first damping fluid drying, first, second test paper laminating or alternate manner is mutual to forming compound test paper, body fluid is dripped on the first test paper, and the reagent of body fluid and the first test paper penetrates on the second test paper, detects the sulfydryl of body fluid.
2. a kind of body fluid sulfydryl detection kit as claimed in claim 1, it is characterized in that: described compound test paper comprises the first compound test paper, the described first compound test paper comprises one first connected epimere test paper and one first hypomere test paper, described the first epimere test paper is made after soaking the first damping fluid drying, and described the first hypomere test paper is made after soaking phosphotungstic acid and the second damping fluid drying.
3. a kind of body fluid sulfydryl detection kit as claimed in claim 2, it is characterized in that: described compound test paper also comprises the second compound test paper, the described second compound test paper paper bag is drawn together one second connected epimere test paper and one second hypomere test paper, described the second epimere test paper is made after soaking the first damping fluid and mercuric chloride drying, and described the second hypomere test paper is made after soaking phosphotungstic acid, mercuric chloride and the second damping fluid drying.
4. a kind of body fluid sulfydryl detection kit as claimed in claim 3, it is characterized in that: also comprise a panel and a base plate, described base plate is provided with the first groove and the second groove of installing respectively the first compound test paper and the second compound test paper, on described panel, corresponding the first groove offers the first well and the first colour developing hole, and corresponding the second groove is provided with the second well and the second colour developing hole.
5. a kind of body fluid sulfydryl detection kit as claimed in claim 4 is characterized in that: described the first well below is the first epimere test paper, and described the first below, colour developing hole is the first hypomere test paper; Described the second well below is the second epimere test paper, and described the second below, colour developing hole is the second hypomere test paper.
6. a kind of body fluid sulfydryl detection kit as claimed in claim 2 or claim 3, is characterized in that: between described the first epimere test paper and the first hypomere test paper, also establish a compartmented; Yet establish a compartmented between described the second epimere paper and described the second hypomere paper.
7. a kind of body fluid sulfydryl detection kit as claimed in claim 1, it is characterized in that: described compound test paper comprises the first compound test paper, the described first compound test paper comprises the first upper strata test paper and the first lower floor's test paper fit together, described the first upper strata test paper is made after soaking the first damping fluid drying, and described first lower floor's test paper is made after soaking phosphotungstic acid and the second damping fluid drying.
8. a kind of body fluid sulfydryl detection kit as claimed in claim 7, it is characterized in that: described compound test paper also comprises the second compound test paper, the described second compound test paper comprises the second upper strata test paper and the second lower floor's test paper fit together, described the second upper strata test paper is made after soaking the first damping fluid and mercuric chloride drying, and described second lower floor's test paper is made after soaking phosphotungstic acid, mercuric chloride and the second damping fluid drying.
9. a kind of body fluid sulfydryl detection kit as claimed in claim 8, it is characterized in that: also comprise a panel and a base plate, offer the first colour developing hole and the second colour developing hole on base plate, on panel, corresponding the first colour developing hole and the second colour developing hole offer respectively the first well and the second well, the first compound test paper is located between the first colour developing hole and the first well, and the second compound test paper is located between the second colour developing hole and the second well.
10. a kind of body fluid sulfydryl detection kit as claimed in claim 9 is characterized in that: described the first well below is the first upper strata test paper, and the below of described first lower floor's test paper is the first colour developing hole; Described the second well below is the second upper strata test paper, and the below of described second lower floor's test paper is the second colour developing hole.
11. a kind of body fluid sulfydryl detection kit, is characterized in that: between described the first upper strata test paper and first lower floor's test paper, also establish a separate layer as claimed in claim 7 or 8; Yet establish a separate layer between described the second upper paper and described second lower floor's paper.
A 12. body fluid detection kit, comprise the first reagent, the second reagent and first, second test paper, it is characterized in that becoming the first test paper after test paper soaks the first reagent drying, another test paper is the second test paper after soaking the second reagent drying, first, second test paper laminating or alternate manner is mutual to forming compound test paper, body fluid is splashed on the first test paper, and the reagent of body fluid and the first test paper penetrates on the second test paper, detects the material of body fluid.
13. a kind of body fluid detection kit according to claim 12, is characterized in that described the first reagent, the second reagent are single dose or are complex reagent.
A 14. body fluid detection kit, comprise the first reagent, the second reagent and first, second adsorbed layer, it is characterized in that becoming the first adsorbed layer after adsorbed layer soaks the first reagent drying, another adsorbed layer is the second adsorbed layer after soaking the second reagent drying, first, second adsorbed layer laminating or alternate manner is mutual to forming the composite adsorption layer, body fluid is splashed on the first adsorbed layer, and the reagent on body fluid and the first adsorbed layer penetrates on the second adsorbed layer, detects the material of body fluid.
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| CN201310274311.2A CN103424402B (en) | 2013-07-02 | 2013-07-02 | A kind of body fluid sulfydryl detection kit |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104089953A (en) * | 2014-07-19 | 2014-10-08 | 青岛贝尔奥生物科技有限公司 | Method for rapidly determining sulfydryl in urine |
| CN104181155A (en) * | 2014-08-10 | 2014-12-03 | 成都煦华生物科技有限公司 | Kit and preparation method thereof |
| CN106468715A (en) * | 2016-06-21 | 2017-03-01 | 安徽康乐美生物科技有限公司 | A kind of cervical cancer urine detection reagent and its preparation method and application |
| CN108051431A (en) * | 2017-11-27 | 2018-05-18 | 深圳瑞达生物股份有限公司 | Thiol compound vitro detection reagent, preparation method and the compound test paper of detection |
| CN114441523A (en) * | 2022-02-10 | 2022-05-06 | 梅宗泉 | Preparation method of kit for detecting sulfhydryl metabolites in urine |
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| CN202092960U (en) * | 2011-05-17 | 2011-12-28 | 李志华 | Cervical carcinoma fast inspection kit |
| CN102944550A (en) * | 2012-10-15 | 2013-02-27 | 上海谱尼测试技术有限公司 | Detection kit for calcium peroxide in flour and detection method for calcium peroxide |
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| CN106468715A (en) * | 2016-06-21 | 2017-03-01 | 安徽康乐美生物科技有限公司 | A kind of cervical cancer urine detection reagent and its preparation method and application |
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