CN103424402B - A kind of body fluid sulfydryl detection kit - Google Patents
A kind of body fluid sulfydryl detection kit Download PDFInfo
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- CN103424402B CN103424402B CN201310274311.2A CN201310274311A CN103424402B CN 103424402 B CN103424402 B CN 103424402B CN 201310274311 A CN201310274311 A CN 201310274311A CN 103424402 B CN103424402 B CN 103424402B
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Abstract
The invention discloses a kind of body fluid sulfydryl detection kit, including phosphotungstic acid, the first buffer and mercuric chloride and first, second reagent paper, reagent paper immersion Salkowski's solution becomes the first reagent paper after being dried, another reagent paper immersion mercuric chloride and the first buffer are the second reagent paper after being dried, first, second reagent paper is fitted or close to each other composition of alternate manner is combined reagent paper, body fluid is dripped on the first reagent paper, the reagent of body fluid and the first reagent paper is penetrated on the second reagent paper, detects the sulfydryl of body fluid.The present invention is compounded with all agent formulations of body fluid sulfydryl detection process, unstable chemical reagent is separated into the first buffer and phosphotungstic acid that can react to each other by mummification is stable with Design of Mechanical Structure, greatly improve reagent stability at normal temperatures, checking process is without the need for any auxiliary reagent and equipment, speed is fast, it is simple to operate, can quickly carry out large-scale Mass screening work.
Description
Technical field
The invention belongs to rapid in-vitro diagnostic kit field, and in particular to a kind of body fluid sulfydryl detection kit.
Background technology
Cervical cancer pathogenesis rate is occupied first of female cancer, at present, clinical practice the detection for cervical cancer and
Diagnostic method has：The inspection of cervix uteri Scrape method, iodine test, cervix uteri and cervical canal biopsy, colposcopy, palace
Conization, TCT detections, high-risk HPV detections etc., but these detection methods and technology all exist be detection process it is complicated,
The professional technique condition of detection has high demands, time length, it is costly the shortcomings of, can only specialist hospital detection use, it is difficult in people
It is widely popularized and uses in group." the portable long-acting fast urine detection of SH test kit " of Lin Genchun et al. inventions is although be one
Quick, the easy method of item, but test kit still suffers from the deficiencies such as composition structure is more complicated, operating procedure is more, and mentioned reagent box
In the reagent holding time is short, stability is poor.
The content of the invention
It is an object of the invention to overcome prior art defect, there is provided a kind of body fluid sulfydryl detection kit.
Technical scheme is as follows：
A kind of body fluid sulfydryl detection kit, including phosphotungstic acid, the first buffer and mercuric chloride and first, second reagent paper,
Reagent paper immersion Salkowski's solution becomes the first reagent paper after being dried, another reagent paper immersion mercuric chloride and the first buffer be dried after for the
Two reagent paper, first, second reagent paper are fitted or close to each other composition of alternate manner are combined reagent paper, body fluid is dripped in the first reagent paper
On, the reagent of body fluid and the first reagent paper is penetrated on the second reagent paper, detects the sulfydryl of body fluid.
In a preferred embodiment of the invention, the compound reagent paper includes the first compound reagent paper, and described first is multiple
Closing reagent paper includes one first connected epimere reagent paper and one first hypomere reagent paper, and the first epimere reagent paper is delayed by immersion first
Rush after liquid is dried and make, the first hypomere reagent paper is made after soaking phosphotungstic acid and the drying of the second buffer.
Preferably, also including the second compound reagent paper, the described second compound reagent paper paper includes connected one to the compound reagent paper
Second epimere reagent paper and one second hypomere reagent paper, the second epimere reagent paper is after soaking the first buffer and mercuric chloride drying
Make, the second hypomere reagent paper is made after soaking the drying of phosphotungstic acid, mercuric chloride and the second buffer.
Preferably, also including a panel and a base plate, the base plate is provided with the first compound reagent paper and second of installing respectively
First groove and the second groove of compound reagent paper, on the panel, the first groove of correspondence offers the first well and the first colour developing
Hole, the second groove of correspondence are provided with the second well and the second colour developing hole.
Preferably, it is the first epimere reagent paper below first well, is the first hypomere below the first colour developing hole
Reagent paper；It is the second epimere reagent paper below second well, is the second hypomere reagent paper below the second colour developing hole.
Preferably, between the first epimere reagent paper and the first hypomere reagent paper, also set a compartmented；The second epimere paper
A compartmented is set between the second hypomere paper yet.
In a preferred embodiment of the invention, the compound reagent paper includes the first compound reagent paper, and described first is multiple
The first upper strata reagent paper and first lower floor's reagent paper that reagent paper includes fitting together are closed, first upper strata reagent paper is by immersion first
Buffer is made after being dried, and first lower floor reagent paper is made after soaking phosphotungstic acid and the drying of the second buffer.
Preferably, also including the second compound reagent paper, the described second compound reagent paper includes fitting together the compound reagent paper
The second upper strata reagent paper and second lower floor's reagent paper, second upper strata reagent paper is after soaking the first buffer and mercuric chloride and being dried
Make, second lower floor reagent paper is made after soaking the drying of phosphotungstic acid, mercuric chloride and the second buffer.
Preferably, also including a panel and a base plate, the first colour developing hole and the second colour developing hole are offered on base plate, on panel
Correspondence first develops the color hole and the second colour developing hole offers the first well and the second well respectively, and the first compound reagent paper is located at the
Between one colour developing hole and the first well, the second compound reagent paper is between the second colour developing hole and the second well.
Preferably, below first well it is the first upper strata reagent paper, the lower section of first lower floor reagent paper is first
Colour developing hole；It is the second upper strata reagent paper below second well, the lower section of second lower floor reagent paper is the second colour developing hole.
Preferably, between first upper strata reagent paper and first lower floor's reagent paper, also set a separate layer；Second upper paper
A separate layer is set yet between second lower floor paper.
Another technical scheme of the present invention is as follows：
A kind of body fluid detection kit, including the first reagent, the second reagent and first, second reagent paper, reagent paper immersion first
Reagent becomes the first reagent paper after being dried, it is the second reagent paper that another reagent paper is soaked after the second reagent is dried, and first, second reagent paper is pasted
Close or close to each other composition of alternate manner is combined reagent paper, body fluid is instilled on the first reagent paper, the reagent of body fluid and the first reagent paper oozes
Enter on the second reagent paper, detect the material of body fluid.
In a preferred embodiment of the invention, the first described reagent, the second reagent are single dose or try for compound
Agent.
The yet another aspect of the present invention is as follows：
A kind of body fluid detection kit, including the first reagent, the second reagent and first, second adsorption layer, adsorption layer immersion
First reagent becomes the first adsorption layer after being dried, and it is the second adsorption layer that another adsorption layer is soaked after the second reagent is dried, by first,
Second adsorption layer fit or alternate manner it is close to each other constitute composite adsorption layer, by body fluid instill the first adsorption layer on, body fluid and
Reagent on first adsorption layer is penetrated on the second adsorption layer, detects the material of body fluid.
The invention has the beneficial effects as follows：
1st, the present invention is compounded with all agent formulations of body fluid sulfydryl detection process, by unstable chemical reagent by dry
Change it is stable the first buffer and phosphotungstic acid that can be reacted to each other is separated with Design of Mechanical Structure, make reagent stablizing at normal temperatures
Property greatly improve, checking process is without the need for any auxiliary reagent and equipment, it is only necessary to distinguish in the first well and the second well
Instill one and drip body fluid sample, after 10 minutes, observe result, speed is fast, simple to operate, can quickly carry out large-scale crowd
Screening；
2nd, simple structure of the invention, with low cost, is adapted to industrialized production.
Description of the drawings
Structural representations of the Fig. 1 for the panel of embodiments of the invention 1
Structural representations of the Fig. 2 for the base plate of embodiments of the invention 1；
Front views of the Fig. 3 for the first compound reagent paper of embodiments of the invention 1；
Fig. 4 is sectional views of the Fig. 3 along line A-A；
Front views of the Fig. 5 for the second compound reagent paper of embodiments of the invention 1；
Fig. 6 is sectional views of the Fig. 5 along line B-B.
Structural representations of the Fig. 7 for the panel of embodiments of the invention 2
Structural representations of the Fig. 8 for the base plate of embodiments of the invention 2；
Front views of the Fig. 9 for the first compound reagent paper of embodiments of the invention 2；
Figure 10 is sectional views of the Fig. 9 along line C-C；
Front views of the Figure 11 for the second compound reagent paper of embodiments of the invention 2；
Figure 12 is sectional views of the Figure 11 along line D-D.
Specific embodiment
Accompanying drawing will be combined below by way of specific embodiment, technical scheme is further detailed and is retouched
State.
Embodiment 1
As depicted in figs. 1 and 2, a kind of body fluid sulfydryl detection kit, including upper one first compound reagent paper 1, one second is again
A panel 3 and a base plate 4 of the reagent paper 2 together with being installed by buckle up and down are closed, base plate 4 is provided with installing respectively first and is combined
First groove 43 and the second groove 44 of reagent paper 1 and the second compound reagent paper 2, on the panel 3, the first groove 43 of correspondence is offered
First well 31 and the first colour developing hole 41, the second groove 44 of correspondence are provided with the second well 32 and the second colour developing hole 42；It is described
Panel forms test kit, or the embedded base plate system in described panel side together with base plate passes through bonding or buckle is installed up and down
Into test kit.
As shown in Figure 3 and Figure 4, the described first compound reagent paper 1 includes one first paper stiff sheet 13, the first paper stiff sheet 13
Be provided with partly overlap together with the first buffer soak be dried formed the first epimere reagent paper 11 and with phosphotungstic acid, second
Buffer immersion is dried the first hypomere reagent paper 12 for being formed, and preferably also sets between the first epimere reagent paper and the first hypomere reagent paper
One compartmented；It is the first epimere reagent paper 11 below first well 31, is the first hypomere below the first colour developing hole 41
Reagent paper 12.
As shown in Figure 5 and Figure 6, the described second compound reagent paper 2 includes one second paper stiff sheet 23, the second paper stiff sheet 23
Be provided with partly overlap together with the first buffer, mercuric chloride immersion be dried formed the second epimere reagent paper 21 and with phosphorus tungsten
The immersion of acid, the second buffer and mercuric chloride is dried the second hypomere reagent paper 23 for being formed, preferably in the second epimere reagent paper and second
A compartmented is set between hypomere reagent paper also；It is the second epimere reagent paper 21 below second well 32, the second colour developing hole
It is the second hypomere reagent paper 22 below 42.
The using method of the present invention is as follows：
Drop women body fluid sample to be detected is respectively dropped in the first well 31 and the second well 32, after 10 minutes
Observation first develops the color the blue reaction in hole 41 and the second colour developing hole 42, when the blueness in the first colour developing hole 41 and the second colour developing hole 42 it is deep
When shallow consistent, be negative findings, that is, show detected person not to be there is evidence that with cervical cancer or precancerous lesion occurs in vivo
（CIN）；When the blueness in the first colour developing hole 41 is deeper than the blueness in the second colour developing hole 42, it is positive findingses, that is, shows tested
Survey person may suffer from cervical cancer, precancerous lesion occurs in vivo（CIN）Or disease relapse after treatment, detected person's reception can be advised
It is further to check to make a definite diagnosis.
Embodiment 2
As shown in Figure 7 and Figure 8, a kind of body fluid sulfydryl detection kit, including upper one first compound reagent paper 1, one second is again
A panel 3 and a base plate 4 of the reagent paper 2 together with installing up and down are closed, the first colour developing hole 41 and the second colour developing on base plate 4, is offered
Hole 42, on panel 3, the first colour developing of correspondence hole 41 and the second colour developing hole 42 offer the first well 31 and the second well respectively
32, the first compound reagent paper 1 is pasted and is connected between the first colour developing hole 41 and the first well 31, and the second compound reagent paper 2 is pasted and connected
It is connected between the second colour developing hole 42 and the second well 32；Described panel, base plate bonding form test kit, or described face
The embedded base plate in plate side makes test kit.
As shown in Figure 9 and Figure 10, the described first compound reagent paper 1 includes overlapping soaking with the first buffer of fitting together
It is dried the first upper strata reagent paper 11 for being formed and first lower floor's reagent paper 12 that formation is dried with phosphotungstic acid and the immersion of the second buffer, institute
It is the first upper strata reagent paper to state below the first well, and the lower section of first lower floor reagent paper is the first colour developing hole；Preferably, it is described
A separate layer is additionally provided between the first upper strata reagent paper and first lower floor's reagent paper.
As is illustrated by figs. 11 and 12, the described second compound reagent paper 2 include overlapping fit together with the first buffer, chlorine
Change hydrargyrum immersion and be dried the second upper strata reagent paper 21 for being formed and soaked with phosphotungstic acid, the second buffer and mercuric chloride and be dried formed the
Two lower floor's reagent paper 22, are the second upper strata reagent paper below second well, and the lower section of second lower floor reagent paper shows for second
Color hole；Preferably, a separate layer is additionally provided between second upper strata reagent paper and second lower floor's reagent paper.
The using method of this test kit is as follows：
Drop women urine specimen to be detected is respectively dropped in the first well 31 and the second well 32, after 10 minutes
Observation first develops the color the blue reaction in hole 41 and the second colour developing hole 42, when the blueness in the first colour developing hole 41 and the second colour developing hole 42 it is deep
When shallow consistent, be negative findings, that is, show detected person not to be there is evidence that with cervical cancer or precancerous lesion occurs in vivo
（CIN）；When the blueness in the first colour developing hole 41 is deeper than the blueness in the second colour developing hole 42, it is positive findingses, that is, shows tested
Survey person may suffer from cervical cancer, precancerous lesion occurs in vivo（CIN）Or disease relapse after treatment, detected person's reception can be advised
It is further to check to make a definite diagnosis.
Embodiment 3
A kind of body fluid detection kit, including the first reagent, the second reagent and first, second reagent paper, reagent paper immersion first
Reagent becomes the first reagent paper after being dried, it is the second reagent paper that another reagent paper is soaked after the second reagent is dried, and first, second reagent paper is pasted
Close or close to each other composition of alternate manner is combined reagent paper, body fluid is instilled on the first reagent paper, the reagent of body fluid and the first reagent paper oozes
Enter on the second reagent paper, detect the material of body fluid.Wherein, the first described reagent, the second reagent are single dose or try for compound
Agent.
Embodiment 4
A kind of body fluid detection kit, including the first reagent, the second reagent and first, second adsorption layer, adsorption layer immersion
First reagent becomes the first adsorption layer after being dried, and it is the second adsorption layer that another adsorption layer is soaked after the second reagent is dried, by first,
Second adsorption layer fit or alternate manner it is close to each other constitute composite adsorption layer, by body fluid instill the first adsorption layer on, body fluid and
Reagent on first adsorption layer is penetrated on the second adsorption layer, detects the material of body fluid.
The above, only presently preferred embodiments of the present invention, therefore the scope of present invention enforcement can not be limited according to this, i.e.,
The equivalence changes made according to the scope of the claims of the present invention and description and modification, all still should belong in the range of the present invention covers.
Claims (14)
1. a kind of body fluid sulfydryl detection kit, including phosphotungstic acid, the first buffer and mercuric chloride and first, second reagent paper, its
It is characterised by：Reagent paper immersion Salkowski's solution becomes the first reagent paper, another reagent paper immersion mercuric chloride and the first buffer after being dried
Be the second reagent paper after drying, compound reagent paper is constituted by first, second reagent paper close to each other, body fluid is dripped on the first reagent paper, body
The reagent of liquid and the first reagent paper is penetrated on the second reagent paper, detects the sulfydryl of body fluid.
2. a kind of body fluid sulfydryl detection kit, including phosphotungstic acid, the first buffer and mercuric chloride and first, second reagent paper, its
It is characterised by：Reagent paper immersion Salkowski's solution becomes the first reagent paper, another reagent paper immersion mercuric chloride and the first buffer after being dried
Be the second reagent paper after drying, compound reagent paper is constituted by first, second reagent paper close to each other, body fluid is dripped on the first reagent paper, body
The reagent of liquid and the first reagent paper is penetrated on the second reagent paper, detects the sulfydryl of body fluid；The compound reagent paper includes that first is combined
Reagent paper and the second compound reagent paper, the described first compound reagent paper include one first connected epimere reagent paper and one first hypomere reagent paper,
The first epimere reagent paper by soak the first buffer be dried after make, the first hypomere reagent paper by soak phosphotungstic acid and
Second buffer is made after being dried, and the described second compound reagent paper includes one second connected epimere reagent paper and the examination of one second hypomere
Paper, the second epimere reagent paper are made after soaking the first buffer and mercuric chloride drying, and the second hypomere reagent paper passes through
Immersion phosphotungstic acid, mercuric chloride and the second buffer are made after being dried.
3. a kind of body fluid sulfydryl detection kit as claimed in claim 2, it is characterised in that：Also include a panel and a bottom
Plate, the base plate are provided with first groove and the second groove of the first compound reagent paper of installing respectively and the second compound reagent paper, described
On panel, the first groove of correspondence offers the first well and the first colour developing hole, and the second groove of correspondence is provided with the second well and the
Two colour developing holes.
4. a kind of body fluid sulfydryl detection kit as claimed in claim 3, it is characterised in that：Below first well it is
First epimere reagent paper, is the first hypomere reagent paper below the first colour developing hole；Try for the second epimere below second well
Paper, is the second hypomere reagent paper below the second colour developing hole.
5. a kind of body fluid sulfydryl detection kit as claimed in claim 2, it is characterised in that：The first epimere reagent paper and
Between one hypomere reagent paper, a compartmented is also set.
6. a kind of body fluid sulfydryl detection kit as claimed in claim 2, it is characterised in that：The second epimere reagent paper and
Between two hypomere reagent paper, a compartmented is additionally provided with.
7. a kind of body fluid sulfydryl detection kit, including phosphotungstic acid, the first buffer and mercuric chloride and first, second reagent paper, its
It is characterised by：Reagent paper immersion Salkowski's solution becomes the first reagent paper, another reagent paper immersion mercuric chloride and the first buffer after being dried
Be the second reagent paper after drying, compound reagent paper is constituted by first, second reagent paper close to each other, body fluid is dripped on the first reagent paper, body
The reagent of liquid and the first reagent paper is penetrated on the second reagent paper, detects the sulfydryl of body fluid；The compound reagent paper includes that first is combined
Reagent paper and the second compound reagent paper, the described first compound reagent paper include the first upper strata reagent paper for fitting together and the examination of the first lower floor
Paper, first upper strata reagent paper are made after soaking the drying of the first buffer, and first lower floor reagent paper is by soaking phosphorus tungsten
Acid and the second buffer are made after being dried, and the described second compound reagent paper is included under the second upper strata reagent paper and second for fitting together
Layer reagent paper, second upper strata reagent paper are made after soaking the first buffer and mercuric chloride drying, second lower floor reagent paper
Made after soaking the drying of phosphotungstic acid, mercuric chloride and the second buffer.
8. a kind of body fluid sulfydryl detection kit as claimed in claim 7, it is characterised in that：Also include a panel and a bottom
Plate, offers the first colour developing hole and the second colour developing hole on base plate, on panel, the first colour developing hole of correspondence and the second colour developing hole are opened respectively
The first well and the second well are provided with, between the first colour developing hole and the first well, second is multiple for the first compound reagent paper
Close reagent paper to develop the color between hole and the second well located at second.
9. a kind of body fluid sulfydryl detection kit as claimed in claim 8, it is characterised in that：Below first well it is
First upper strata reagent paper, the lower section of first lower floor reagent paper is the first colour developing hole；It is the second upper strata below second well
Reagent paper, the lower section of second lower floor reagent paper is the second colour developing hole.
10. a kind of body fluid sulfydryl detection kit as claimed in claim 7, it is characterised in that：First upper strata reagent paper with
Between first lower floor's reagent paper, a separate layer is also set.
A kind of 11. body fluid sulfydryl detection kits as claimed in claim 7, it is characterised in that：Second upper strata reagent paper with
Between second lower floor's reagent paper, a separate layer is also set.
A kind of 12. body fluid detection kit, including the first reagent, the second reagent and first, second reagent paper, it is characterised in that reagent paper
Soaking after the first reagent is dried becomes the first reagent paper, and it is the second reagent paper that another reagent paper is soaked after the second reagent is dried, by first, the
Two reagent paper are close to each other to constitute compound reagent paper, and body fluid is instilled on the first reagent paper, and the reagent of body fluid and the first reagent paper penetrates into the
On two reagent paper, the material of body fluid is detected.
13. a kind of body fluid detection kit according to claim 12, it is characterised in that described the first reagent, the second examination
Agent is single dose or is complex reagent.
A kind of 14. body fluid detection kit, including the first reagent, the second reagent and first, second adsorption layer, it is characterised in that inhale
Attached layer soaks becomes the first adsorption layer after the first reagent is dried, another adsorption layer is soaked after the second reagent is dried for the second absorption
Layer, composition composite adsorption layer first, second adsorption layer close to each other instills body fluid on the first adsorption layer, body fluid and first
Reagent on adsorption layer is penetrated on the second adsorption layer, detects the material of body fluid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310274311.2A CN103424402B (en) | 2013-07-02 | 2013-07-02 | A kind of body fluid sulfydryl detection kit |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310274311.2A CN103424402B (en) | 2013-07-02 | 2013-07-02 | A kind of body fluid sulfydryl detection kit |
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| CN103424402A CN103424402A (en) | 2013-12-04 |
| CN103424402B true CN103424402B (en) | 2017-03-29 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104089953A (en) * | 2014-07-19 | 2014-10-08 | 青岛贝尔奥生物科技有限公司 | Method for rapidly determining sulfydryl in urine |
| CN104181155A (en) * | 2014-08-10 | 2014-12-03 | 成都煦华生物科技有限公司 | Kit and preparation method thereof |
| CN106468715A (en) * | 2016-06-21 | 2017-03-01 | 安徽康乐美生物科技有限公司 | A kind of cervical cancer urine detection reagent and its preparation method and application |
| CN108051431A (en) * | 2017-11-27 | 2018-05-18 | 深圳瑞达生物股份有限公司 | Thiol compound vitro detection reagent, preparation method and the compound test paper of detection |
| CN114441523A (en) * | 2022-02-10 | 2022-05-06 | 梅宗泉 | Preparation method of kit for detecting sulfhydryl metabolites in urine |
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| CN2602374Y (en) * | 2003-02-26 | 2004-02-04 | 林根春 | Uterocarcinoma rapid self-checking agent case |
| CN201438190U (en) * | 2009-07-02 | 2010-04-14 | 林根春 | Portable kit for long-time effective and fast detection of sulfhydryl in body fluids |
| CN202092960U (en) * | 2011-05-17 | 2011-12-28 | 李志华 | Cervical carcinoma fast inspection kit |
| CN102944550A (en) * | 2012-10-15 | 2013-02-27 | 上海谱尼测试技术有限公司 | Detection kit for calcium peroxide in flour and detection method for calcium peroxide |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3341152B2 (en) * | 1998-09-17 | 2002-11-05 | 中部電力株式会社 | Asbestos screening method |
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|---|---|---|---|---|
| CN2602374Y (en) * | 2003-02-26 | 2004-02-04 | 林根春 | Uterocarcinoma rapid self-checking agent case |
| CN201438190U (en) * | 2009-07-02 | 2010-04-14 | 林根春 | Portable kit for long-time effective and fast detection of sulfhydryl in body fluids |
| CN202092960U (en) * | 2011-05-17 | 2011-12-28 | 李志华 | Cervical carcinoma fast inspection kit |
| CN102944550A (en) * | 2012-10-15 | 2013-02-27 | 上海谱尼测试技术有限公司 | Detection kit for calcium peroxide in flour and detection method for calcium peroxide |
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