CN201477101U - Transferrin and hemoglobin combined detection board - Google Patents
Transferrin and hemoglobin combined detection board Download PDFInfo
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- CN201477101U CN201477101U CN2009201579317U CN200920157931U CN201477101U CN 201477101 U CN201477101 U CN 201477101U CN 2009201579317 U CN2009201579317 U CN 2009201579317U CN 200920157931 U CN200920157931 U CN 200920157931U CN 201477101 U CN201477101 U CN 201477101U
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- haemoglobin
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- transferrins
- test strip
- monoclonal antibody
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Abstract
The utility model relates to a transferrin and hemoglobin combined detection board, belonging to the field of medical detection. The detection board comprises an upper shell, a lower shell and test strips; two sample adding holes and two observation windows are arranged on the surface of the upper shell; the two test strips are arranged between the upper shell and the lower shell; grooves corresponding to the test strips are arranged inside the lower shell; and the two test strips are respectively a transferring detection test strip and a hemoglobin detection test strip. Each detection test strip comprises a substrate, a water absorption plate, a nitrocellulose membrane, a color grain storage pad containing transferrin monoclonal antibodies or hemoglobin monoclonal antibodies, and a sample liquid adsorption layer, wherein the nitrocellulose membrane is provided with a transferrin monoclonal antibody or hemoglobin monoclonal antibody detection line and a goat anti-mouse polyclonal antibody control line which are separated from each other. The detection board can detect the hemoglobin and the transferrin contained in the excrement at the same time and can remarkably improve the positive detection rate of hemorrhagic diseases in the alimentary canal.
Description
Technical field
The utility model relates to a kind of check-out console, relate in particular to a kind of transferrins, haemoglobin joint-detection plate, it is hemorrhage to judge whether alimentary canal has by the content of transferrins in the mensuration ight soil and haemoglobin, can help the colorectal cancer early prediction, belongs to medical detection range.
Background technology
The detection of fecal occult blood has very significant values for the hemorrhage diagnosis in chronic digestible road and the screening of large intestine malignant tumour.The method that detection at present commonly used is just occulted blood is mainly two kinds of chemical method and immunizations.
The ultimate principle of chemical method is, hemosiderin in the haemoglobin partly has the effect of catalysis peroxide breakdown, and the hydrogen peroxide in the energy catalytic reagent decomposes the release new ecological oxygen, what of haemoglobin the above-mentioned chromogen substance of oxidation and colour generation, the depth of colour generation have reacted.Chemical method is easy to detect, the result shows fast, clear, but because ight soil composition difference is very big, there is very big-difference in each laboratory concrete operations details difference with the chemical method testing result in actual applications, and lack accuracy and specificity, false positive reaction often occurs.
The index that immunization detects is haemoglobin and transferrins.The immunization of haemoglobin monoclonal antibody measuring is compared with traditional chemical method, and sensitivity, specificity have all had large increase, and the influence of unable to take food thing or related drugs, has been widely used in having a medical check-up of clinical and batch crowd.But stabilized hemoglobin is poor, and Chang Yin is subjected to the mucus composition influence that the effect of intestinal bacterium and big intestinal mucosa produce and false negative reaction appears in sex change.Transferrins mainly is present in the blood plasma, exists hardly in healthy people's ight soil, also can be used as the detection index of fecal occult blood.The susceptibility of transferrins detection method and specificity and haemoglobin detect quite, but stability is apparently higher than haemoglobin, and it is long and be decomposed or destroy the false negative result that the back occurs to overcome haemoglobin hold-up time in intestines and stomach in the tested sample.Present domestic immunization detects more with haemoglobin, be easy to cause upper gastrointestinal bleeding to fail to pinpoint a disease in diagnosis.Therefore, application combined immunization detection method detects haemoglobin and the transferrins in the ight soil simultaneously, and the positive rate of digestion being said courageous and upright disease is significantly increased.
Summary of the invention
The purpose of this utility model is to overcome the deficiencies in the prior art, and a kind of transferrins, haemoglobin joint-detection plate are provided, thereby detects when realizing transferrins and haemoglobin, improves the positive rate of hemorrhage of digestive tract disease greatly.
In order to solve the problems of the technologies described above, the utility model is achieved through the following technical solutions:
The utility model relates to a kind of transferrins, haemoglobin joint-detection plate, comprise upper casing, lower casing and test strips, the upper casing surface is provided with two wells and two view windows, two test strips place between upper casing and the lower casing, lower casing inside is provided with and the corresponding groove of test strips, and wherein two test strips are respectively transferrins test strip and haemoglobin test strip.
The transferrins test strip is made up of base plate, water sucting plate, nitrocellulose filter, the colored particle storage pad and the sample liquid-adsorption layer that contain the transferrins monoclonal antibody, is loaded with the transferrins monoclonal antibody detection line and the sheep anti mouse polyclonal antibody control line that are separated from each other on the nitrocellulose filter.Wherein colored particle can be a kind of colloid gold particle, collargol particle, CI particle, magnetic-particle, dye granule, latex particle or fluorescent grain, preferred colloid gold particle or latex particle.
The haemoglobin test strip is made up of base plate, water sucting plate, nitrocellulose filter, the colored particle storage pad and the sample liquid-adsorption layer that contain the haemoglobin monoclonal antibody, is loaded with the haemoglobin monoclonal antibody detection line and the sheep anti mouse polyclonal antibody control line that are separated from each other on the described nitrocellulose filter.Wherein colored particle can be a kind of colloid gold particle, collargol particle, CI particle, magnetic-particle, dye granule, latex particle or fluorescent grain, preferred colloid gold particle or latex particle.
Because adopt technique scheme, transferrins provided by the utility model, haemoglobin joint-detection plate have such beneficial effect: 1, high specificity, highly sensitive, easily store, need not the technical skill personnel operation, do not need instrument and equipment, and readability as a result; 2, can detect transferrins and haemoglobin simultaneously, improve the positive rate of hemorrhage of digestive tract disease greatly.
Description of drawings
Fig. 1 is a schematic appearance of the present utility model;
Fig. 2 is an exploded view of the present utility model;
Fig. 3 is the outside drawing of test strip in the utility model;
Fig. 4 is the exploded view of test strip in the utility model;
Fig. 5 is the negative findings figure of test strip in the utility model;
Fig. 6 is the positive findings figure of test strip in the utility model.
Description of reference numerals: 1, upper casing, 2, lower casing, 3, well, 4, view window, 5, groove, 6, the transferrins test strip, 7, haemoglobin test strip, 8, base plate, 9, water sucting plate, 10, nitrocellulose filter, 11, colored particle storage pad, 12, sample liquid-adsorption layer, 13, detection line, 14, control line
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is described in further detail:
Embodiment 1:
A kind of transferrins, haemoglobin joint-detection plate, comprise upper casing 1, lower casing 2 and test strips, upper casing 1 surface is provided with two wells 3 and two view windows 4, two test strips place between upper casing 1 and the lower casing 2, lower casing 2 inside are provided with and the corresponding groove 5 of test strips, and wherein two test strips are respectively transferrins test strip 6 and haemoglobin test strip 7.
Transferrins test strip 6 is made up of base plate 8, water sucting plate 9, nitrocellulose filter 10, the colored particle storage pad 11 and the sample liquid-adsorption layer 12 that contain the transferrins monoclonal antibody, base plate 8 middle parts are nitrocellulose filter 10, are loaded with the transferrins monoclonal antibody detection line 13 and the sheep anti mouse polyclonal antibody control line 14 that are separated from each other on the nitrocellulose filter 10.In base plate 8 one end terminations is water sucting plate 9, other end termination is a sample liquid-adsorption layer 12, nitrocellulose filter 10 two ends overlap mutually with the colored particle storage pad 11 that contains the transferrins monoclonal antibody with water sucting plate 9 respectively and are connected, and are pressed with sample liquid-adsorption layer 12 on colored particle storage pad 11.Wherein, colored particle is a colloid gold particle, and corresponding colored particle storage pad is gold mark pad.
During detection, sample is splashed into two wells respectively, because the capillarity sample will move to water sucting plate along test strips, when moving to gold mark pad, transferrins antigen in the sample (or haemoglobin antigen) forms compound with transferrins monoclonal antibody label probe (or haemoglobin monoclonal antibody label probe) the generation specific bond that the gold mark fills up, when moving to detection line, transferrins antigen in the sample (or haemoglobin antigen) combines with transferrins monoclonal antibody (or haemoglobin monoclonal antibody) in the detection line again and forms the double-antibody sandwich immune complex, therefore its collaurum is stranded on the detection line, form a red stripes (T line), the transferrins monoclonal antibody (or haemoglobin monoclonal antibody) of remaining mark collaurum can continue to move, because it derives from mouse, therefore can combine with the sheep anti mouse polyclonal antibody and form an obvious colour band (C line), promptly no matter whether contain transferrins (or haemoglobin) in the sample, gold mark probe all can combine delay with the sheep anti mouse polyclonal antibody of bag quilt on the nitrocellulose filter, and it is red that control line is shown.Therefore, when on the transferrins test strip, seeing detection line (T line) and control line (C line), the content that proves transferrins in the detected sample is greater than 40ng/ml, the result is positive, only when control line (C line) locates to occur a colour band, the result is negative, and the content that transferrins in the detected sample is described is less than 40ng/ml; When on the haemoglobin test strip, seeing detection line (T line) and control line (C line), the content that proves haemoglobin in the detected sample is greater than 0.2 μ g/ml, the result is positive, only when control line (C line) locates to occur a colour band, the result is negative, and the content that haemoglobin in the detected sample is described is less than 0.2 μ g/ml; Any one test strips shows positive findings in transferrins test strip and the haemoglobin test strip, and it is hemorrhage to show that all alimentary canal has, and has improved the positive rate of hemorrhage of digestive tract disease so greatly.
Embodiment 2:
A kind of transferrins, haemoglobin joint-detection plate, comprise upper casing 1, lower casing 2 and test strips, upper casing 1 surface is provided with two wells 3 and two view windows 4, two test strips place between upper casing 1 and the lower casing 2, lower casing 2 inside are provided with and the corresponding groove 5 of test strips, and wherein two test strips are respectively transferrins test strip 6 and haemoglobin test strip 7.
Transferrins test strip 6 is made up of base plate 8, water sucting plate 9, nitrocellulose filter 10, the colored particle storage pad 11 and the sample liquid-adsorption layer 12 that contain the transferrins monoclonal antibody, base plate 8 middle parts are nitrocellulose filter 10, are loaded with the transferrins monoclonal antibody detection line 13 and the sheep anti mouse polyclonal antibody control line 14 that are separated from each other on the nitrocellulose filter 10.In base plate 8 one end terminations is water sucting plate 9, other end termination is a sample liquid-adsorption layer 12, nitrocellulose filter 10 two ends overlap mutually with the colored particle storage pad 11 that contains the transferrins monoclonal antibody with water sucting plate 9 respectively and are connected, and are pressed with sample liquid-adsorption layer 12 on colored particle storage pad 11.Wherein, colored particle is a latex particle, and corresponding colored particle storage pad is the latex pad.
It is identical with detection principle among the embodiment 1 to detect principle.
Claims (4)
1. a transferrins, haemoglobin joint-detection plate, comprise upper casing (1), lower casing (2) and test strips, upper casing (1) surface is provided with two wells (3) and two view windows (4), two test strips place between upper casing (1) and the lower casing (2), lower casing (2) inside is provided with and the corresponding groove of test strips (5), it is characterized in that: described two test strips are respectively transferrins test strip (6) and haemoglobin test strip (7).
2. transferrins according to claim 1, haemoglobin joint-detection plate, it is characterized in that: described transferrins test strip (6) is made up of base plate (8), water sucting plate (9), nitrocellulose filter (10), the colored particle storage pad (11) and the sample liquid-adsorption layer (12) that contain the transferrins monoclonal antibody, is loaded with the transferrins monoclonal antibody detection line (13) and the sheep anti mouse polyclonal antibody control line (14) that are separated from each other on the described nitrocellulose filter (10).
3. transferrins according to claim 1, haemoglobin joint-detection plate, it is characterized in that: described haemoglobin test strip (7) is made up of base plate (8), water sucting plate (9), nitrocellulose filter (10), the colored particle storage pad (11) and the sample liquid-adsorption layer (12) that contain the haemoglobin monoclonal antibody, is loaded with the haemoglobin monoclonal antibody detection line (13) and the sheep anti mouse polyclonal antibody control line (14) that are separated from each other on the described nitrocellulose filter (10).
4. according to claim 2 or 3 described transferrinss, haemoglobin joint-detection plate, it is characterized in that: described colored particle can be a kind of colloid gold particle, collargol particle, CI particle, magnetic-particle, dye granule, latex particle or fluorescent grain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201579317U CN201477101U (en) | 2009-06-04 | 2009-06-04 | Transferrin and hemoglobin combined detection board |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201579317U CN201477101U (en) | 2009-06-04 | 2009-06-04 | Transferrin and hemoglobin combined detection board |
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| CN201477101U true CN201477101U (en) | 2010-05-19 |
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| CN2009201579317U Expired - Lifetime CN201477101U (en) | 2009-06-04 | 2009-06-04 | Transferrin and hemoglobin combined detection board |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103529207A (en) * | 2013-10-17 | 2014-01-22 | 天津中新科炬生物制药有限公司 | Combined device for rapidly and quantitatively detecting multiple tumor markers |
| CN104515859A (en) * | 2014-12-17 | 2015-04-15 | 杭州慧缘泰医疗器械有限公司 | Hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit and preparation method and detection method thereof |
| CN105467124A (en) * | 2015-12-18 | 2016-04-06 | 厦门稀土材料研究所 | Colloid gold duplex test strip for detecting fecal occult blood and preparation method thereof |
| CN105911276A (en) * | 2016-07-12 | 2016-08-31 | 中国人民解放军南京军区福州总医院 | Seven-link gold label detection card and preparation method thereof |
| CN106290824A (en) * | 2016-10-19 | 2017-01-04 | 中国科学院电子学研究所 | Two-parameter fan-shaped immunity magnetosphere analysis strip |
| CN106546744A (en) * | 2015-09-17 | 2017-03-29 | 上海透景生命科技股份有限公司 | By the method and corresponding reagent box of fecal hemoglobin, transferrins and PKM2 joint-detection assessing colorectal cancers |
| CN108020665A (en) * | 2016-10-31 | 2018-05-11 | 联华生技股份有限公司 | Set group and sampling gimmick are tested in upper and lower digestive system cancer screening |
| CN114200143B (en) * | 2020-09-18 | 2024-04-02 | 上海透景诊断科技有限公司 | Application of hemoglobin and transferrin in detecting digestive tract hemorrhage |
-
2009
- 2009-06-04 CN CN2009201579317U patent/CN201477101U/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103529207A (en) * | 2013-10-17 | 2014-01-22 | 天津中新科炬生物制药有限公司 | Combined device for rapidly and quantitatively detecting multiple tumor markers |
| CN104515859A (en) * | 2014-12-17 | 2015-04-15 | 杭州慧缘泰医疗器械有限公司 | Hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit and preparation method and detection method thereof |
| CN106546744A (en) * | 2015-09-17 | 2017-03-29 | 上海透景生命科技股份有限公司 | By the method and corresponding reagent box of fecal hemoglobin, transferrins and PKM2 joint-detection assessing colorectal cancers |
| CN105467124A (en) * | 2015-12-18 | 2016-04-06 | 厦门稀土材料研究所 | Colloid gold duplex test strip for detecting fecal occult blood and preparation method thereof |
| CN105911276A (en) * | 2016-07-12 | 2016-08-31 | 中国人民解放军南京军区福州总医院 | Seven-link gold label detection card and preparation method thereof |
| CN106290824A (en) * | 2016-10-19 | 2017-01-04 | 中国科学院电子学研究所 | Two-parameter fan-shaped immunity magnetosphere analysis strip |
| CN108020665A (en) * | 2016-10-31 | 2018-05-11 | 联华生技股份有限公司 | Set group and sampling gimmick are tested in upper and lower digestive system cancer screening |
| CN114200143B (en) * | 2020-09-18 | 2024-04-02 | 上海透景诊断科技有限公司 | Application of hemoglobin and transferrin in detecting digestive tract hemorrhage |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20100519 |
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| CX01 | Expiry of patent term |