CN208537564U - A kind of A rotavirus/Adenovirus Antigen detection kit - Google Patents
A kind of A rotavirus/Adenovirus Antigen detection kit Download PDFInfo
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- CN208537564U CN208537564U CN201820156875.4U CN201820156875U CN208537564U CN 208537564 U CN208537564 U CN 208537564U CN 201820156875 U CN201820156875 U CN 201820156875U CN 208537564 U CN208537564 U CN 208537564U
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Abstract
The utility model describes a kind of A rotavirus/Adenovirus Antigen detection kit and its component, setting detection card in kit, component includes sample collecting device, the sample collecting device includes adopting just bottle and adopting just stick, it adopts just bottle and is provided with buffer, the swab for adopting just stick and including centre with adopting the swivel that just bottle rotation connects, the broken tip and bottom end that top is set, swab end two sides is all oval, and it is uniformly distributed several salient points, there is broken tip on the just stick top of adopting, and a siphunculus is formed after fractureing.The traditional operation bring that the utility model avoids pollutes cumbersome with sub-sampling again.Have the function of quick, easy, do not need special instrument and equipment, is suitable for the insufficient outlying mountain area of medical condition, basic hospital clinic, family, bedside detection etc..It has a good application prospect in technical field of immunoassay.
Description
Technical field
The utility model belongs to technical field of immunoassay, is related to a kind of A rotavirus/Adenovirus Antigen detection reagent
Box.
Background technique
Rotavirus (Rotavirus, abbreviation RV) is a kind of diplornavirus, belongs to Reoviridae.Wheel
Shape virus finished Sharp (Ruth Bishop) discovery by the Rose in Australia for the first time in 1973, the same period cause 50% infant because
Severe diarrhea and the case of hospitalization.Rotavirus is produced by the protection of intestinal mucosal barrier cells (enterocyte) of infection and small intestine connection
Raw enterotoxin (enterotoxin) leads to enterogastritis, causes serious diarrhea, or even dead.
Infant infection diarrhea caused by A rotavirus mostly occurs in autumn and winter, is mainly passed with fecal oral route
It broadcasts, can also directly be propagated by person to person, contaminated articles are propagated indirectly.Clinical manifestation can be transient mild diarrhea, can also
To be severe gastro-enteritis, cause de- water and rock-soil coupling, shock even threat to life.
Intestinal adenovirus category Adenoviridae is one of the main pathogen for leading to infant's virus diarrhea.Intestinal adenovirus
It is that Flewett is found in the stool sample of electricity consumption sem observation acute gastroenteritis infant for the first time.Intestinal adenovirus infection is in the whole world
Property distribution, have been reported that all over the world.Research finds that the Acute Infantile Diarrhea as caused by intestinal adenovirus accounts for 1.1% ~ 12.0%.
Research shows that adenovirus enteritis, without obvious seasonal characteristic, whole year can fall ill, route of transmission is mainly that mouth-excrement is propagated.Enteron aisle gland
Viral main infringement 5 years old or less children, 85% or more intestinal adenovirus the infected are 3 years old hereinafter, 70% intestinal adenovirus the infected
For 2 years old or less children, domestic morbidity in studies have shown that adenovirus diarrhea 2 years old or less accounted for the 86.7% of total morbidity number of cases.
Intestinal adenovirus was infected with 10 days or so incubation periods, and cardinal symptom is diarrhea after the onset, be in watery stool or loose stools,
Amount is more or less.Defecation frequency can up to 8~9 times/day, and the course of disease general 4~8 days.Most of patients has fever with vomiting, minority
(atypia adenovirus infection patient fever is more), about 20% adenovirus enteritis patient has respiratory symptom.The adenovirus enteritis state of an illness
Generally relatively light, minority has serious dehydration or needs hospitalization with other systems infection.
Clinically mainly there are cell isolated culture, gel electrophoresis, ferment for the detection method of A rotavirus at present
Plain immunochromatographic method, RT-PCR method, latex agglutination test.Detection method for adenovirus mainly has: cell isolated culture,
Hemagglutination-inhibition test, latex agglutination test, fluorescent immune method, enzyme-linked immunization.
After rotavirus enters human body, can largely it rise in value in the villus cell matter of enteron aisle, so that villus cell is destroyed, and
Human body can be discharged with excrement with the cell that necrosis falls off.Villus cell will lead to acute diarrhea, collective's damage after considerable damage
Dehydration point and electrolyte, serious person may occur in which the clinical symptoms such as acid poisoning, dehydration and shock.Answered after childhood infection rotavirus and
When diagnosis and treatment, life danger is likely to occur after otherwise largely losing electrolyte and body fluid, so clinical want the detection of rotavirus
Ask answer it is quick and accurate.
If cannot be early diagnosed whether if bacterium or virus infection in diarrhea, it is considered that diarrhea is drawn by bacterium infection
It rises, then blindly using or be transfused antibiotic to infant can also aggravate disease not only without positive effect.Because of antibiosis
Element use cause normal intestinal flora to be lacked of proper care, for virus duplication create condition, be diarrhea the more control the more heavy so that
Dehydration, or even life danger occurs.Virus can survive a couple of days in dry conditions, and the wet environment lower time-to-live is longer, infection
Clinical manifestation difference is very big afterwards, and it is different diarrhea can be dehydrated from laxativeness to lethal.
The detection kit for being commonly used for detection rotavirus and adenovirus in the market is not furnished with sample collecting device generally,
After usually being collected with common disposable stool cup, it is dissolved in the pipe equipped with dilution with cotton swab picking, sufficiently uses liquid relief after reaction
Rifle or Dispette absorption are added drop-wise on detection card, and so multiple mobile sample may cause sample in moving process
Pollution, the contact surface of cotton swab picking than relatively limited, during being drawn by Dispette may residual miscellaneous fecal sample, to rear
Continuous detection card reaction adversely affects.Clinically mainly there is cell separation training for the detection method of A rotavirus at present
Support method, gel electrophoresis, ferment immunochromatographic method, RT-PCR method, latex agglutination test.Detection method for adenovirus is main
Have: cell isolated culture, hemagglutination-inhibition test, latex agglutination test, fluorescent immune method, enzyme-linked immunization.
But the detection reagent component for being commonly used for detection rotavirus and adenovirus in the market is not adopted generally equipped with sample
Acquisition means are dissolved in the pipe equipped with dilution, sufficiently after reaction after usually being collected with common disposable stool cup with cotton swab picking
It is added drop-wise on detection gets stuck with liquid-transfering gun or Dispette absorption, so multiple mobile sample, it may in moving process
The pollution of sample is caused, the contact surface of cotton swab picking, may residual miscellaneous excrement during being drawn by Dispette than relatively limited
Sample adversely affects subsequent detection reaction of getting stuck.
Summary of the invention
The technical problem to be solved by the utility model is to provide a kind of A rotavirus/Adenovirus Antigen detection reagents
Box avoids the problem of repeatedly transhipment sample pollutes.
For this purpose, the utility model uses following technical scheme:
Setting A rotavirus/Adenovirus Antigen detection card, the component include sample collection dress in the kit
It sets, the sample collecting device includes adopting just bottle and adopting just stick, and the just bottle of adopting is provided with buffer, described to adopt during just stick includes
Between with the swab of adopting the swivel of just bottle rotation connection, the broken tip and bottom end that top is set, the swab end two
Face is all oval, and is uniformly distributed several salient points, carries out multidraw to different location during conveniently adopting just, puts back to after sampling
Just bottle is adopted equipped with buffer, adopts the hollow plastic on just stick top by fractureing after completely dissolution, liquid therefrom vacancy after inversion
Dropwise addition process is realized in outflow；
There is broken tip on the just stick top of adopting, and a siphunculus is formed after fractureing；Detection card include shell and
Immuno-chromatographic test paper strip, the immuno-chromatographic test paper strip, the immuno-chromatographic test paper strip include plastic backings and are arranged in plastics
The nitrocellulose filter for the setting successively up pasted on backing, colloidal gold pad, water absorption pad, sample pad.
On the basis of using above technical scheme, the utility model can also be using scheme further below:
Setting sample-adding window and watch window, the test strips are divided into sample application zone, reaction zone and water suction, institute on the shell
It is corresponding with sample-adding window to state sample application zone, the reaction zone is corresponding with watch window, and the reaction zone is provided with p-wire T line
And nature controlling line C line.
The sample-adding window and the watch window are rounded corner, have do not puncture, pattern is beautiful, sample-adding uniformly and on
Under get stuck button bit more closely.On the surface text that gets stuck do silk-screen processing, there is permanent, friction not fade feature；The shell table
Face text does silk-screen processing.
The watch window is equipped with the independent A rotavirus instruction area not interfered and adenovirus instruction area.
The p-wire includes detection line T1 and detection line T2, and detection line T1 is the anti-wheel being coated on nitrocellulose filter
Shape viral monoclonal antibodies are constituted, and detection line T2 is the anti-adenovirus monoclonal antibody being coated on nitrocellulose filter.
Corresponding immunochromatography reagent position is divided into three regions under the observation window, is rotavirus instruction respectively
Area, adenovirus instruction area and internal control indicate that area, internal control instruction area are presented aubergine and indicate that product is effective, rotavirus instruction
Area, gland shape virus instruction area are presented aubergine and indicate in sample whether contain rotavirus, adenovirus.
It is described to adopt just bottle and adopt just stick and screw connection.
Kit described in the utility model is formed by detecting to get stuck with sample collecting device, detects the interior immunochromatography that gets stuck
Reagent strip can detect two kinds of ingredients simultaneously, and sample collection device can realize that sample reacts in a closed system, not by outer
Boundary's factor influences.It is clear and accurate to read result, while having many advantages, such as that easy to operate, high sensitivity, specificity are good, is suitable for doctor
The insufficient outlying mountain area for the treatment of condition, basic hospital, personal detection or family's detection etc., product market prospects are good.
Detailed description of the invention
Fig. 1 is that A rotavirus/Adenovirus Antigen detects the figure that gets stuck.
Fig. 2 is to detect the interior immuno-chromatographic test paper strip front schematic diagram that gets stuck.
Fig. 3 is to detect the interior immuno-chromatographic test paper strip side view that gets stuck.
Fig. 4 is sample collecting device front elevation.
Specific embodiment
Below in conjunction with drawings and examples, the present invention will be further described in detail.
As shown in Figure 1, the utility model includes cuboid plastic shell 1 and immuno-chromatographic test paper strip 2, shell 1 divides for face
Plate and bottom plate two parts, by upper and lower concave-convex card slot come button bit, test strips 2 are fixed on by bottom plate groove to get stuck in 1.On panel
Equipped with sample-adding window 6 and watch window 7, it is equipped with rotavirus in watch window 7 and indicates area 3, adenovirus instruction area 4 and internal control refer to
Show area 5.
Immuno-chromatographic test paper strip 2 includes sample application zone 8, reaction zone 9, the suction zones 10 set gradually, wherein sample application zone 8 and face
6 position of sample-adding window on plate is corresponding, and reaction zone 9 is corresponding with watch window 7, and reaction zone has two p-wire T lines and matter
Control line C line.As shown in Figure 2.
As shown in figure 3, side is seen, immuno-chromatographic test paper strip 2 includes plastic backings 11, sample pad 15, colloidal gold pad 14, nitre
Acid cellulose film 13 and water absorption pad 12, plastic backings 11 be successively pasted with from bottom to top nitrocellulose filter 13, colloidal gold pad 14,
Sample pad 15, water absorption pad 12.There are coating nature controlling line and detection line on nitrocellulose filter.
Wherein nature controlling line C is that the sheep anti-mouse igg polyclonal antibody being coated on nitrocellulose filter is constituted, and detection line T1 is
The anti-rotavirus monoclonal antibody being coated on nitrocellulose filter is constituted, and detection line T2 is to be coated on nitrocellulose filter
Anti- adenovirus monoclonal antibody.
Corresponding immunochromatography reagent item (2) position is divided into three regions under the watch window (7), is A groups respectively
Rotavirus indicates that area (3), adenovirus instruction area (4) and internal control indicate area (5), the corresponding detection line T in three regions when detection
With nature controlling line C using present aubergine as react mark.
Detection shell is a flat rectangular body, including plastic shell, immuno-chromatographic test paper strip two parts, and plastics get stuck two
End, sample-adding window and watch window are rounded corner, have and do not puncture, pattern beauty, are loaded uniformly and get stuck button bit more up and down
It is close.Upper case surface text does silk-screen processing, has permanent, friction not fade feature.Immuno-chromatographic test paper strip, test strips by
Under it is supreme be plastic backings, nitrocellulose filter, colloidal gold pad, sample pad, water absorption pad respectively.
Specifically, the plastic shell is divided into two pieces, i.e. panel and bottom plate up and down.The panel and bottom plate inner face are equipped with recessed
Salient point and tongue and groove, it can be achieved that two plates close fastening, get stuck button bit up and down more closely.The survey of the immuno-chromatographic test paper strip
The anti-A rotavirus monoclonal antibody of coating mouse and adenovirus monoclonal antibody on line are tried, is coated with sheep anti-mouse igg on nature controlling line,
Gold mark rotavirus monoclonal antibody and gold mark adenovirus monoclonal antibody in colloidal gold pad.
A rotavirus described in the utility model/Adenovirus Antigen detection reagent component, once can be same after sample-adding
The Indicator Reaction of A rotavirus and adenovirus is carried out in root test strips simultaneously, reaction result can be observed by observation window, whole
It only need to be once loaded in a detection process, overcoming leads to the non-uniform disadvantage of sample content by being loaded twice, and can understand straight
That sees observes test result.
Meanwhile the utility model provides a kind of matched sample collecting device, adopting just stick bottom end is with certain area
Ellipsoid, be uniformly distributed salient point above, mainly conveniently adopt just during to different location carry out multidraw.The device with
Common sampling cotton swab or swab are compared, expand sampling area can multidraw, fractureed after sampling by device top, allow processing
Good sample is added drop-wise on reagent gets stuck by fracture, the device avoids the pollution of traditional operation bring and again sub-sampling
It is cumbersome.
When detection, just 17 ellipsoid of stick, 18 multipoint acquisition fecal sample is adopted in sample collection device, is put into after acquisition
Adopting in just bottle 16 equipped with buffer keeps closed by screw thread, and reaction in a moment, fractures and adopts the tip 19 on just stick top, by it
It is inverted and 3 drop samples is added dropwise into sample-adding window 6.Sample is chromatographed under capillary effect by sample application zone-reaction zone-suction zones direction.Such as
When containing wheel virus antigen in tested sample, gold mark rotavirus antibody forms compound in conjunction with wheel virus antigen,
" Au-Rota-Ab-Rota-Ag-Rota- is formed in conjunction with the anti-rotavirus antibody being fixed at detection line in chromatography process
Ab " centre-fills, to an aubergine band occur in detection zone T1；Conversely, detection zone T1 does not occur aubergine band.Together
No matter reason such as is detected to be detected in sample in sample and contains adenovirus, and gold mark adenovirus antibody is formed again in conjunction with Adenovirus Antigen
Object is closed, forms " Au-Adeno-Ab-Adeno- in conjunction with the Anti-adenovirus antibody being fixed at detection line in chromatography process
Ag-Adeno-Ab " centre-fills, to an aubergine band occur in detection zone T2；Conversely, detection zone T2 do not occur it is purplish red
Vitta band.Regardless of whether compound can all continue up chromatography to control zone C, with sheep anti mouse there are Rota or Adeno antigen
There is an aubergine band in IgG reaction.The aubergine band that control zone C is presented is to judge whether chromatography process is normally marked
Standard, while also as the inner quality standard of reagent.
Although the content of the utility model is discussed in detail through the foregoing embodiment, it should be appreciated that on
Stating description should not be considered as limitations of the present invention.After those skilled in the art have read above content, for
The multiple modifications and substitutions of the utility model will be apparent, therefore be also included in the scope of the utility model.
Claims (6)
1. a kind of A rotavirus/Adenovirus Antigen detection kit, it is characterised in that A groups of colyliforms are arranged in the kit
Virus/Adenovirus Antigen detection card, the kit includes sample collecting device, and the sample collecting device includes adopting just bottle
(16) and just stick (17) are adopted, the just bottle (16) of adopting is provided with buffer, and the just stick (17) of adopting includes that centre is revolved with just bottle is adopted
Turn the swab (18) of the swivel of connection, the broken tip (19) and bottom end that top is set, swab (18) end two
Face is all oval, and is uniformly distributed several salient points, and described just stick (17) top of adopting has broken tip (19), is formed after fractureing
A piece siphunculus；
The detection card includes shell (1) and immuno-chromatographic test paper strip (2), the immuno-chromatographic test paper strip (2), the immune layer
Analysis test strips (2) includes plastic backings (11) and the cellulose nitrate that the setting successively up pasted on plastic backings (11) is arranged
Plain film (13), colloidal gold pad (14), water absorption pad (12), sample pad (15).
2. a kind of A rotavirus/Adenovirus Antigen detection kit according to claim 1, it is characterised in that described
Setting sample-adding window (6) and watch window (7), the test strips are divided into sample application zone (8), reaction zone (9) and suction zones on shell
(10), the sample application zone (8) is corresponding with sample-adding window (6), and the reaction zone (9) is corresponding with watch window (7), described anti-
Area (9) is answered to be provided with p-wire T line and nature controlling line C line.
3. a kind of A rotavirus/Adenovirus Antigen detection kit according to claim 2, it is characterised in that described
It is loaded window (6) and the watch window (7) is rounded corner, the case surface text does silk-screen processing.
4. a kind of A rotavirus/Adenovirus Antigen detection kit according to claim 2, it is characterised in that described
Watch window (7) is equipped with independent A rotavirus instruction area (3) and adenovirus instruction area (4) that do not interfere.
5. a kind of A rotavirus/Adenovirus Antigen detection kit according to claim 2, it is characterised in that described
P-wire includes detection line T1 and detection line T2, and detection line T1 is the anti-rotavirus monoclonal being coated on nitrocellulose filter
Antibody is constituted, and detection line T2 is the anti-adenovirus monoclonal antibody being coated on nitrocellulose filter.
6. a kind of A rotavirus/Adenovirus Antigen detection kit according to claim 1, it is characterised in that described
It adopts just bottle (16) and adopts just stick (17) and screw connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820156875.4U CN208537564U (en) | 2018-01-30 | 2018-01-30 | A kind of A rotavirus/Adenovirus Antigen detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820156875.4U CN208537564U (en) | 2018-01-30 | 2018-01-30 | A kind of A rotavirus/Adenovirus Antigen detection kit |
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| Publication Number | Publication Date |
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| CN208537564U true CN208537564U (en) | 2019-02-22 |
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| CN201820156875.4U Active CN208537564U (en) | 2018-01-30 | 2018-01-30 | A kind of A rotavirus/Adenovirus Antigen detection kit |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110018313A (en) * | 2019-04-10 | 2019-07-16 | 江苏硕世生物科技股份有限公司 | A kind of A group wheel virus antigen test strip, kit and preparation method thereof |
| CN110940806A (en) * | 2019-11-18 | 2020-03-31 | 广东医科大学附属医院 | Adenovirus and rotavirus quantum dot joint detection test strip and preparation method and application thereof |
| CN112274185A (en) * | 2019-07-22 | 2021-01-29 | 大连中孚生物工程有限公司 | Excrement sample collection method and auxiliary excrement sample collection device |
| WO2021203254A1 (en) * | 2020-04-08 | 2021-10-14 | 杭州莱和生物技术有限公司 | Test strip carrier plate, test device and manufacturing method therefor |
-
2018
- 2018-01-30 CN CN201820156875.4U patent/CN208537564U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110018313A (en) * | 2019-04-10 | 2019-07-16 | 江苏硕世生物科技股份有限公司 | A kind of A group wheel virus antigen test strip, kit and preparation method thereof |
| CN112274185A (en) * | 2019-07-22 | 2021-01-29 | 大连中孚生物工程有限公司 | Excrement sample collection method and auxiliary excrement sample collection device |
| CN110940806A (en) * | 2019-11-18 | 2020-03-31 | 广东医科大学附属医院 | Adenovirus and rotavirus quantum dot joint detection test strip and preparation method and application thereof |
| WO2021203254A1 (en) * | 2020-04-08 | 2021-10-14 | 杭州莱和生物技术有限公司 | Test strip carrier plate, test device and manufacturing method therefor |
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