CN201302572Y - Acute myocardial infarction assay kit - Google Patents
Acute myocardial infarction assay kit Download PDFInfo
- Publication number
- CN201302572Y CN201302572Y CNU2008201559145U CN200820155914U CN201302572Y CN 201302572 Y CN201302572 Y CN 201302572Y CN U2008201559145 U CNU2008201559145 U CN U2008201559145U CN 200820155914 U CN200820155914 U CN 200820155914U CN 201302572 Y CN201302572 Y CN 201302572Y
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- myocardial infarction
- acute myocardial
- lid
- nitrocellulose filter
- detection kit
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Abstract
The utility model relates to an acute myocardial infarction assay kit including a bottom plate, test strips and a box cover, two mutually parallel slots are formed on the bottom plate thereof, the human cardiac troponin I test strip and the glycogen phosphorylase isoenzyme BB test strip are respectively arranged in the two slots, the slots or bumps corresponding to the box cover are formed on the bottom plate, the bottom plate can be buckled with the box cover, two sample adding holes and two observation windows are formed on the box cover. The acute myocardial infarction assay kit can simultaneously qualitatively detect two indicators of human cardiac troponin I and glycogen phosphorylase isoenzyme BB, simplify the operation and make up for the lack of the traditional diagnostic method of myocardial infarction, therefore the acute myocardial infarction assay kit has great significance for early screening of acute myocardial infarction.
Description
Technical field
The utility model relates to a kind of kit that detects usefulness, is specifically related to a kind of acute myocardial infarction AMI detection kit.
Background technology
Cardiac muscle troponin I (cTnI) is the subunit of cardiac troponin compound, is present on the myocardium myo fibrillation actin filament, and relative molecular mass is 24000, is cardiac muscle cell's its specific structure albumen.Under normal circumstances, the content of cTnI is very low in the blood, when some pathologies cause myocardial cell injury, in the rapid disperse human blood of cTnI, causes that the content of cTnI raises rapidly in the blood.Big quantity research CTnT has been proved to be one of special, the most responsive index of myocardial damage, is diagnosing acute myocardial infarction (acute myocardial infarction, " goldstandard " AMI).
Glycogen phosphorylase (glycogen phosphorylase, GPBB) be glycogenolytic key enzyme, constitute the decomposition of glycogen compound at cardiac muscle cell's sarcoplasmic reticulum, this compound is responsive especially to the decomposition of glycogen that ischemic causes, its formation is changed, form soluble g PBB, permeate through cell membranes enters blood, early diagnosis to acute myocardial ischemia is better than cardiogram, is patient's AMI pectoralgia of finding at present the most responsive index in back 4 hours of showing effect.
Assay method commonly used has radioimmunoassay method, high pressure liquid chromatographic analysis method, Biochemical Analyzer and various diagnostic kit.These methods need instrument and professional's outfit, and during operating cost.Simultaneously traditional fast check reagent bar can only detect an index, and in the practice, often needs to detect simultaneously a plurality of indexs, adopts kit that a plurality of individual events detect and reagent strip to operate respectively and just seems loaded down with trivial details time-consuming.
Summary of the invention
The technical problems to be solved in the utility model is to provide a kind of acute myocardial infarction AMI detection kit that detects human cardiac troponin I/glycogen phosphorylase isoenzyme BB simultaneously, operates more easy.At cTnI and GPBB characteristics to the AMI diagnosis, GPBB and myocardium specificity marker thing cTnI are combined, will have absolute specificity and high susceptibility to the early diagnosis of myocardial ischemia.
For solving the problems of the technologies described above, the utility model has adopted following technical proposals:
A kind of acute myocardial infarction AMI detection kit, comprise base plate, reagent strip and lid, wherein, base plate is provided with two draw-in grooves that are parallel to each other, place human cardiac troponin I detectable bar and glycogen phosphorylase isoenzyme BB detectable bar in two draw-in grooves respectively, base plate is provided with draw-in groove corresponding with lid or projection, and base plate can fasten with lid, and lid is provided with two wells and two observation windows.
Above-mentioned human cardiac troponin I detectable bar comprises bottom bracket, adsorptive pads, nitrocellulose filter, all-glass paper and sample pad, wherein, sample pad, all-glass paper, nitrocellulose filter and adsorptive pads tandem array and being fixed on the bottom bracket successively.In the human cardiac troponin I detectable bar, nitrocellulose filter is corresponding with a watch window of lid, and sample pad is corresponding with a well of lid.Nitrocellulose filter is provided with the detection line that is coated with the cardiac muscle troponin I monoclonal antibody and is coated with the nature controlling line of sheep anti mouse polyclonal antibody, is fixed with cardiac muscle troponin I monoclonal antibody collaurum potpourri on the all-glass paper.Above-mentioned detection line and nature controlling line are parallel to each other, the nearly application of sample nose end of detection line wherein, and nature controlling line is away from the application of sample nose end.
Above-mentioned glycogen phosphorylase isoenzyme BB detectable bar comprises bottom bracket, adsorptive pads, nitrocellulose filter, all-glass paper and sample pad, wherein, sample pad, all-glass paper, nitrocellulose filter and adsorptive pads tandem array and being fixed on the bottom bracket successively.In the glycogen phosphorylase isoenzyme BB detectable bar, nitrocellulose filter is corresponding with a watch window of lid, and described sample pad is corresponding with a well of lid.Nitrocellulose filter is provided with the detection line that is coated with glycogen phosphorylase isoenzyme BB monoclonal antibody and the nature controlling line that is coated with the sheep anti mouse polyclonal antibody, fixing glycogen phosphorylase isoenzyme BB monoclonal antibody collaurum potpourri on the all-glass paper.Above-mentioned detection line and nature controlling line are parallel to each other, the nearly application of sample nose end of detection line wherein, and nature controlling line is away from the application of sample nose end.
The in-vitro diagnosis that the utility model can be used for human cardiac troponin I/glycogen phosphorylase isoenzyme BB detects, and is significant to the early screening of acute myocardial infarction AMI.
Description of drawings
Fig. 1: acute myocardial infarction AMI detectable box plate synoptic diagram
Fig. 2: acute myocardial infarction AMI detection kit lid synoptic diagram
Fig. 3: human cardiac troponin I detectable and glycogen phosphorylase isoenzyme BB detectable bar structural representation
Fig. 4: human cardiac troponin I detectable and glycogen phosphorylase isoenzyme BB detectable bar side view
Embodiment
Acute myocardial infarction AMI detection kit as illustrated in fig. 1 and 2, comprise base plate 1, reagent strip and lid 2, wherein, base plate 1 is provided with two draw-in grooves that are parallel to each other 3,4, place human cardiac troponin I detectable bar 5 and glycogen phosphorylase isoenzyme BB detectable bar 6 in two draw-in grooves respectively, base plate is provided with draw-in groove corresponding with lid or projection, and base plate can fasten with lid, and lid is provided with well 7 and observation window 8.
Human cardiac troponin I detectable bar structure is shown in Fig. 3 and 4, comprise bottom bracket 9, adsorptive pads 10, nitrocellulose filter 11, all-glass paper 12 and sample pad 13, wherein, sample pad 13, all-glass paper 12, nitrocellulose filter 11 and adsorptive pads 10 tandem array and being fixed on the bottom bracket 9 successively.In the human cardiac troponin I detectable bar, nitrocellulose filter 11 is corresponding with the watch window 8 of lid, and sample pad 13 is corresponding with the well 7 of lid.Nitrocellulose filter 11 is provided with detection line 14 that is coated with the cardiac muscle troponin I monoclonal antibody and the nature controlling line 15 that is coated with the sheep anti mouse polyclonal antibody, is fixed with cardiac muscle troponin I monoclonal antibody collaurum potpourri on the all-glass paper 12.Above-mentioned detection line 14 and nature controlling line 15 are parallel to each other, detection line 14 nearly well 7 ends wherein, and nature controlling line 15 is away from well 7 ends.
Above-mentioned glycogen phosphorylase isoenzyme BB detectable bar structure is shown in Fig. 3 and 4, comprise bottom bracket 9, adsorptive pads 10, nitrocellulose filter 11, all-glass paper 12 and sample pad 13, wherein, sample pad 13, all-glass paper 12, nitrocellulose filter 11 and adsorptive pads 10 tandem array and being fixed on the bottom bracket 9 successively.In the glycogen phosphorylase isoenzyme BB detectable bar, nitrocellulose filter 11 is corresponding with the watch window 8 of lid, and described sample pad 13 is corresponding with the well 7 of lid.Nitrocellulose filter 11 is provided with the detection line 14 that is coated with glycogen phosphorylase isoenzyme BB monoclonal antibody and is coated with the nature controlling line 15 of sheep anti mouse polyclonal antibody, fixing glycogen phosphorylase isoenzyme BB monoclonal antibody collaurum potpourri on the all-glass paper 12.Above-mentioned detection line 14 and nature controlling line 15 are parallel to each other, detection line 14 nearly well 7 ends wherein, and nature controlling line 15 is away from well 7 ends.The material that uses in the above-mentioned acute myocardial infarction AMI detection kit makes as follows:
1. MONOCLONAL ANTIBODIES SPECIFIC FOR
With people cardiac muscular tissue is that main raw material(s) extracts purifying cTnI and GPBB respectively as immunogene, and immune BALB/c mouse respectively is through initial immunity and booster immunization repeatedly.Separate the splenocyte of mouse and carry out Fusion of Cells, make hybridoma with SP2/0 myeloma cell.Cultivation filters out anti-cTnI cell line of two strains and the anti-GPBB cell line of two strains.After the injection mouse peritoneal induced ascites, purifying got cardiac muscle troponin I monoclonal antibody I, II and glycogen phosphorylase isoenzyme BB monoclonal antibody I, II.
2. colloid gold particle and monoclonal antibody combines
Cardiac muscle troponin I monoclonal antibody I, glycogen phosphorylase isoenzyme BB monoclonal antibody I is marked at respectively on the red colloid gold particle, is fixed on the all-glass paper drying for standby.
3. bag is by nitrocellulose filter
Nature controlling line is by sheep anti mouse polyclonal antibody bag quilt, and detection line wraps respectively by glycogen phosphorylase isoenzyme BB monoclonal antibody II, cardiac muscle troponin I monoclonal antibody II.
Above-mentioned acute myocardial infarction AMI detection kit using method:
Draw the blood serum sample with the application of sample suction pipe, drip 3 (about 120ul) blood serum samples then in each well of kit.The a different sample of every detection is noted using different suction pipes.Sentence read result in 15 minutes after dripping sample.Only in negative result of red lines of the nature controlling line of watch window (C line) position appearance as a result.Two colour bands occurring in the watch window as a result, promptly red lines, positive result respectively appear in detection line (T line) and nature controlling line (C line) position.Have in the expression sample and surpass myocardium calcium protein I or the glycogen phosphorylase isoenzyme BB existence that detects sensitivity.Nature controlling line (C line) does not occur showing that test result is uncertain, answers retry.
Claims (9)
1. acute myocardial infarction AMI detection kit, comprise base plate, reagent strip and lid, it is characterized in that, base plate is provided with two draw-in grooves that are parallel to each other, place human cardiac troponin I detectable bar and glycogen phosphorylase isoenzyme BB detectable bar in two draw-in grooves respectively, base plate is provided with draw-in groove corresponding with lid or projection, and base plate can fasten with lid, and lid is provided with two wells and two observation windows.
2. acute myocardial infarction AMI detection kit as claimed in claim 1, it is characterized in that, described human cardiac troponin I detectable bar comprises bottom bracket, adsorptive pads, nitrocellulose filter, all-glass paper and sample pad, wherein, sample pad, all-glass paper, nitrocellulose filter and adsorptive pads tandem array and being fixed on the bottom bracket successively.
3. acute myocardial infarction AMI detection kit as claimed in claim 2 is characterized in that, described nitrocellulose filter is corresponding with a watch window of lid, and described sample pad is corresponding with a well of lid.
4. acute myocardial infarction AMI detection kit as claimed in claim 2, it is characterized in that, described nitrocellulose filter is provided with the detection line that is coated with the cardiac muscle troponin I monoclonal antibody and is coated with the nature controlling line of sheep anti mouse polyclonal antibody, is fixed with cardiac muscle troponin I monoclonal antibody collaurum potpourri on the described all-glass paper.
5. acute myocardial infarction AMI detection kit as claimed in claim 4 is characterized in that described detection line and nature controlling line are parallel to each other, the nearly application of sample nose end of detection line wherein, and nature controlling line is away from the application of sample nose end.
6. acute myocardial infarction AMI detection kit as claimed in claim 1, it is characterized in that, described glycogen phosphorylase isoenzyme BB detectable bar comprises bottom bracket, adsorptive pads, nitrocellulose filter, all-glass paper and sample pad, wherein, sample pad, all-glass paper, nitrocellulose filter and adsorptive pads tandem array and being fixed on the bottom bracket successively.
7. acute myocardial infarction AMI detection kit as claimed in claim 6 is characterized in that, described nitrocellulose filter is corresponding with a watch window of lid, and described sample pad is corresponding with a well of lid.
8. acute myocardial infarction AMI detection kit as claimed in claim 6, it is characterized in that, described nitrocellulose filter is provided with the detection line that is coated with glycogen phosphorylase isoenzyme BB monoclonal antibody and the nature controlling line that is coated with the sheep anti mouse polyclonal antibody, fixing glycogen phosphorylase isoenzyme BB monoclonal antibody collaurum potpourri on the described all-glass paper.
9. acute myocardial infarction AMI detection kit as claimed in claim 8 is characterized in that described detection line and nature controlling line are parallel to each other, the nearly application of sample nose end of detection line wherein, and nature controlling line is away from the application of sample nose end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201559145U CN201302572Y (en) | 2008-11-25 | 2008-11-25 | Acute myocardial infarction assay kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201559145U CN201302572Y (en) | 2008-11-25 | 2008-11-25 | Acute myocardial infarction assay kit |
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|---|---|
| CN201302572Y true CN201302572Y (en) | 2009-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNU2008201559145U Expired - Fee Related CN201302572Y (en) | 2008-11-25 | 2008-11-25 | Acute myocardial infarction assay kit |
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| CN (1) | CN201302572Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103930783A (en) * | 2011-11-16 | 2014-07-16 | 皇家飞利浦有限公司 | Particle repulsion to enhance surface contact in magnetic particle immunoassays |
| CN104897631A (en) * | 2015-06-01 | 2015-09-09 | 上海凯创生物技术有限公司 | Near infrared fluorescence detection kit for glycogen phosphorylase isoenzyme BB |
| CN106290883A (en) * | 2015-06-01 | 2017-01-04 | 上海凯创生物技术有限公司 | GPBB colloidal gold method detection kit |
| CN106526176A (en) * | 2016-12-05 | 2017-03-22 | 无锡艾科瑞思产品设计与研究有限公司 | Multifunctional fluorescence-immunochromatography rapid quantitative detection strip |
-
2008
- 2008-11-25 CN CNU2008201559145U patent/CN201302572Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103930783A (en) * | 2011-11-16 | 2014-07-16 | 皇家飞利浦有限公司 | Particle repulsion to enhance surface contact in magnetic particle immunoassays |
| CN104897631A (en) * | 2015-06-01 | 2015-09-09 | 上海凯创生物技术有限公司 | Near infrared fluorescence detection kit for glycogen phosphorylase isoenzyme BB |
| CN106290883A (en) * | 2015-06-01 | 2017-01-04 | 上海凯创生物技术有限公司 | GPBB colloidal gold method detection kit |
| CN104897631B (en) * | 2015-06-01 | 2018-01-09 | 上海凯创生物技术有限公司 | GPBB near-infrared fluorescent method detection kit |
| CN106290883B (en) * | 2015-06-01 | 2018-06-29 | 上海凯创生物技术有限公司 | Glycogen phosphorylase isoenzyme BB colloidal gold method detection kit |
| CN106526176A (en) * | 2016-12-05 | 2017-03-22 | 无锡艾科瑞思产品设计与研究有限公司 | Multifunctional fluorescence-immunochromatography rapid quantitative detection strip |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090902 Termination date: 20111125 |