CN204405675U - A kind of mycoplasma pneumoniae IgM antibody test card - Google Patents
A kind of mycoplasma pneumoniae IgM antibody test card Download PDFInfo
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- CN204405675U CN204405675U CN201420811000.5U CN201420811000U CN204405675U CN 204405675 U CN204405675 U CN 204405675U CN 201420811000 U CN201420811000 U CN 201420811000U CN 204405675 U CN204405675 U CN 204405675U
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Abstract
The utility model provide a kind of easy and simple to handle, testing cost is low, the chlamydia pneumoniae IgM antibody test card of highly sensitive, high specificity, it comprises plastics and gets stuck and test paper part, describedly get stuck for flat rectangular parallelepiped, the upper surface that gets stuck is provided with view window and well successively, test paper is fixed on inside of getting stuck, and described view window and well are all sealed with removable plastic dividing plate.After have employed the utility model scheme, provide a kind of mycoplasma pneumoniae IgM antibody test card, test strips is not polluted before the funnel-shaped design of removable plastic dividing plate and well ensure that application of sample, do not cause cross pollution during application of sample, can know and observe test result intuitively.Meanwhile, the utility model is simple to operate, react quick, highly sensitive, specificity is good, can realize the detection to mycoplasma pneumoniae, for clinically to the preliminary examination of suspicious mycoplasma pneumoniae infection crowd.
Description
Technical field
The utility model belongs to the category that Medical Immunology detects, and relates to a kind of test card of mycoplasma pneumoniae IgM antibody.
Background technology
Mycoplasma pneumoniae (Mycoplasma Pneumoniae, MP) is the important pathogen body causing childrens respiratory tract infection disease, can cause after infection comprise heart, the extrapulmonary complication of multi viscera that central nervous system unifies urinary system and system.According to statistics, from 0.6%-47% not etc., MP accounts for 70% of community-acquired pneumonia in children cause of disease, is most Susceptible population, and has the trend of reach with 4-20 year for the incidence of disease of MP.The four seasons all can fall ill, and decline multiple with resistibility in winter in spring, can cycle eruption and prevalence.
The morbidity of the pathogenesis of MP mainly airway epithelial cell absorption mechanism: MP is mainly by droplet transmission, after pathogen is entered by respiratory tract, combine closely at mucous membrane surface and the epithelial neuraminic acid acceptor of respiratory mucosa and adhere to, thus causing the destruction of mucosal epithelium, this is the principal causative mode of MP.If the neuraminic acid acceptor of artificial suppression mucosal epithelial cells, the epithelial cell damage caused by MP can greatly weaken.Meanwhile, MP has similar antigenic component to mucomembranous epithelial cell film, escape the immunosurveillance (being called " MP immunologic escape ") of host cell, firmly be adsorbed on mucomembranous surface, not easy-clear, and its toxic metabolic products can be discharged, as ammonia, hydrogen peroxide, proteinase and neurotoxin etc., the pathology of corresponding site can be caused.There is scholar once from the blood of MP respiratory tract infection person, to be directly separated to MP, also have scholar to damage bleb liquid and pleural effusion from skin and cultivate MP, prove that MP arrives any organ-tissue of whole body by hematogenous spread.
The clinical manifestation of MP is varied, and without classical symptom, X ray sign lacks specificity, is difficult to cause infection to differentiate mutually with other pathogen.As can not Timeliness coverage take effective control measure, may be in progress as respiratory tract infection, bronchitis, pneumonia and extrapulmonary complication.IgM antibody is the specific antibody of early diagnosis mycoplasma pneumoniae.MP first produces IgM antibody in body after infecting, and generally within one week, occur after infection, 3-4 week reaches peak, reduces gradually later, and 12-16 week turns out cloudy.And IgG antibody will symptom occur after within 7-10 days, just can be detected, therefore MP IgG antibody cannot be applied to MP infect early diagnosis, be used for retrospective diagnosis.The early diagnosis of MP and early treatment can shorten the course of disease, prevent the generation of complication, and laboratory examination is the important diagnosis basis that MP infects.
The laboratory diagnostic method of current use has MP to be separated cultivation, Serologic detection and nuclease PCR etc.Be separated the goldstandard that cultivation is diagnose infections, but complex operation, length consuming time, technical requirement is high; Nuclease PCR needs specific installation, expensive, and Grass-roots Hospital is more difficult, and therefore both are all not suitable for the clinical principium diagnosis that MP infects.Serologic detection comprises cold agglutination test, complement fixation test (CFT), gelatin particle agglutination, ELISA method, MG streptococcus agglutination test, colloidal gold immunity chromatography and immunofluorescence technique.Wherein colloidal gold immunity chromatography and ELISA method are the effective means of detection mycoplasma pneumoniae relatively more conventional in domestic laboratory method, ELISA method detects needs professional and equipment, use underaction, and colloidal gold immunity chromatography has quick detection in 5 minutes, simple operation and other advantages, is applicable to, to the early stage preliminary examination of suspicious mycoplasma pneumoniae infection, detecting positive case, bind nucleic acid PCR test or cell chulture do further validation test, to clarify a diagnosis again.
Summary of the invention
Technical problem to be solved in the utility model is to provide a kind of chlamydia pneumoniae IgM antibody test card, can not pollute watch window and well before application of sample, can mycoplasma pneumoniae IgM antibody in qualitative detection human serum sample, can be used for the auxiliary diagnosis of clinical mycoplasma pneumoniae infection.There is the advantages such as easy and simple to handle, testing cost is low, highly sensitive, high specificity, necessary emergency treatment can be carried out in the outlying mountain area of medical condition deficiency, basic hospital, clinic.
The utility model is by the following technical solutions: it comprises plastics and gets stuck and test paper part, describedly get stuck for flat rectangular parallelepiped, the upper surface that gets stuck is provided with view window and well successively, test paper is fixed on inside of getting stuck, described view window and well are all sealed with removable plastic dividing plate, described well and view window be provided with removable plastic dividing plate and funnel-shaped design have prevent application of sample before or effect that in application of sample process, test strips is polluted.
Described get stuck comprise get stuck and under get stuck, above get stuck for panel, under get stuck for base plate, the test side of panel is provided with one section of polished surface.
Get stuck on described and under get stuck by buckle close, the upper surface that gets stuck on described is provided with two inward recess, one for bottom opening size be the infundibulate well of 6mm*2.5mm, one for bottom opening size be the rectangular view window of 15mm*3mm, the sample that the hopper design of well can prevent disposable overload from causing expose described under to get stuck the point-like groove that plastic boss that inside is provided with starlike distribution forms, described point-like groove, in order to fixing test strips, prevents the skew of test strips.
On the basis adopting technique scheme, the utility model can also adopt following further scheme:
Nitrocellulose filter, colloidal gold pad and sample pad that described test paper 2 comprises plastic backings and pastes successively in plastic backings.
Described test paper 2 is fixed in point-like groove 6, is disposed with sample application zone, reaction zone, suction zones, and wherein view window 3 is corresponding with the position of reaction zone, and well 4 is corresponding with the position of sample application zone.
Detection line on described nitrocellulose filter is coated with mouse-anti people-IgM μ chain antibody, nature controlling line is coated with anti-MP antibody.
After have employed the utility model scheme, provide a kind of mycoplasma pneumoniae IgM antibody test card, test strips is not polluted before the funnel-shaped design of removable plastic dividing plate and well ensure that application of sample, do not cause cross pollution during application of sample, can know and observe test result intuitively.Meanwhile, the utility model is simple to operate, react quick, highly sensitive, specificity is good, can realize the detection to mycoplasma pneumoniae, for clinically to the preliminary examination of suspicious mycoplasma pneumoniae infection crowd.
Accompanying drawing explanation
Fig. 1 is general structure schematic diagram of the present utility model.
Fig. 2 is the inner side figure of the utility model base plate.
Fig. 3 is the inner side figure of the utility model panel.
Embodiment
Below in conjunction with drawings and Examples, the utility model is further elaborated.
The utility model comprises plastics and to get stuck 1 and test paper 2 part, 1 is got stuck for flat rectangular parallelepiped described in it is characterized in that, 1 upper surface that gets stuck is provided with view window 3 and well 4 successively, test paper 2 is fixed on 1 inside of getting stuck, described view window 3 and well 4 are all sealed with removable plastic dividing plate 5, described get stuck 1 comprise get stuck and under get stuck, get stuck on described and under get stuck by buckle close, the upper surface that gets stuck on described is provided with two inward recess, one for bottom opening size be the infundibulate well 4 of 6mm*2.5mm, one for bottom opening size be the rectangular view window 3 of 15mm*3mm, get stuck under described inside be provided with starlike distribution plastic boss form point-like groove 6, described point-like groove (6) is in order to fixing test strips, the sample that the hopper design of well can prevent disposable overload from causing exposes, get stuck under described inside be provided with starlike distribution plastic boss form point-like groove (6), described point-like groove (6) is in order to fixing test strips, prevent the skew of test strips.
Described plastic casing be divided into get stuck and under get stuck, i.e. panel and base plate.Described panel and base plate inner face are provided with sags and crests and tongue and groove, can realize the tight fastening of two plates.On the get stuck test side of (panel) be provided with one section of polished cross-sections, easy-to-hold, test strips is fixed in the point-like plastic channel of base plate, good stability, and test strips is plastic backings, nitrocellulose filter, colloidal gold pad, sample pad, adsorptive pads from the bottom to top respectively.
Test paper 2 is fixed in point-like groove 6, is disposed with sample application zone, reaction zone, suction zones, and wherein view window 3 is corresponding with the position of reaction zone, and well 4 is corresponding with the position of sample application zone.
Embodiment:
A kind of mycoplasma pneumoniae IgM antibody test card, as shown in Figure 1, comprise plastic casing 1 and immuno-chromatographic test paper strip 2, shell 1 is flat rectangular body, base plate and panel are fitted together to by salient point and groove, panel is divided into test lead and handheld terminal, test lead is provided with well 4 and view window 3, test strips 2 is fixed in base plate by bottom plate groove 6, described well 4 and view window 3 removable plastic baffle plate 5 seal, pull open plastic dam during application of sample and carry out application of sample, test, observation test result, avoid the front pollution to test strips of application of sample or the improper cross pollution caused of application of sample, handheld terminal establishes one section of polished cross-sections, have anti-skidding, the function of easy-to-hold.
See inside base plate, the salient point of distribution of being scattered by varying in size forms groove in order to fixing test strips, test strips 2 is not easily offset, immuno-chromatographic test paper strip 2 comprise set gradually sample application zone, reaction zone, suction zones, wherein sample application zone is corresponding with well 4 position on panel, reaction zone is corresponding with the view window 3 on panel, and there are p-wire T line and nature controlling line C line in reaction zone.
Immuno-chromatographic test paper strip 2 comprises plastic backings, sample pad, colloidal gold pad, nitrocellulose filter and adsorptive pads, and plastic backings is pasted with nitrocellulose filter, colloidal gold pad, sample pad, adsorptive pads successively.Nitrocellulose filter has bag by nature controlling line and detection line, view window 3 is corresponding with the position of nitrocellulose filter, and well 4 is corresponding with the position of sample pad.Wherein nature controlling line is that the anti-Mp antibody be coated on nitrocellulose filter is formed, and wrapping tested survey line is that the mouse-anti people-IgM μ chain antibody be coated on nitrocellulose filter is formed, using the MP recombinant antigen of colloid gold label as cue mark thing.
During detection, serum sample is chromatography under capillary effect.During as contained MP IgM antibody in tested sample, gold mark MP recombinant antigen is combined with MP IgM antibody and forms compound, be combined with the mouse-anti people-IgM μ chain antibody being fixed on detection line place in chromatography process and form " Au-MP-Ag-MP-IgM-mouse-anti people-IgM μ chain antibody " centre-fills, thus occur an aubergine band at detection zone (T); Otherwise there is not aubergine band in detection zone (T).No matter whether there is MP antibody in tested sample, compound all can continue upwards chromatography and, to control zone (C), occur an aubergine band with anti-MP antibody response.The aubergine band that control zone (C) presents judges the whether normal standard of chromatography process, simultaneously also as the inner quality standard of reagent.
More than the utility model describe and should not be considered to restriction of the present utility model.To repeatedly apparent amendment of the present utility model and substitute, be also included within scope of the present utility model.
Claims (4)
1. a mycoplasma pneumoniae IgM antibody test card, it comprises plastics and gets stuck (1) and test paper (2) part, (1) is got stuck for flat rectangular parallelepiped described in it is characterized in that, (1) upper surface that gets stuck is provided with view window (3) and well (4) successively, it is inner that test paper (2) is fixed on get stuck (1), described view window (3) and well (4) are all sealed with removable plastic dividing plate (5), described get stuck (1) comprise get stuck and under get stuck, on get stuck for panel, under get stuck for base plate, get stuck on described and under get stuck by buckle close, the upper surface that gets stuck on described is provided with two inward recess, one for bottom opening size be the infundibulate well (4) of 6mm*2.5mm, one for bottom opening size be the rectangular view window (3) of 15mm*3mm, get stuck under described inside be provided with starlike distribution plastic boss form point-like groove (6), described point-like groove (6) is in order to fixing test strips, the test side of panel is provided with one section of polished surface.
2. a kind of mycoplasma pneumoniae IgM antibody test card according to claim 1, is characterized in that nitrocellulose filter, colloidal gold pad and sample pad that described test paper (2) comprises plastic backings and pastes successively in plastic backings.
3. a kind of mycoplasma pneumoniae IgM antibody test card according to claim 1 and 2, it is characterized in that described test paper (2) is fixed in point-like groove (6), be disposed with sample application zone, reaction zone, suction zones, wherein view window (3) is corresponding with the position of reaction zone, and well (4) is corresponding with the position of sample application zone.
4. a kind of mycoplasma pneumoniae IgM antibody test card according to claim 2, is characterized in that the detection line on described nitrocellulose filter being coated with mouse-anti people-IgM μ chain antibody, nature controlling line is coated with anti-MP antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420811000.5U CN204405675U (en) | 2014-12-18 | 2014-12-18 | A kind of mycoplasma pneumoniae IgM antibody test card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420811000.5U CN204405675U (en) | 2014-12-18 | 2014-12-18 | A kind of mycoplasma pneumoniae IgM antibody test card |
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| CN204405675U true CN204405675U (en) | 2015-06-17 |
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| CN201420811000.5U Active CN204405675U (en) | 2014-12-18 | 2014-12-18 | A kind of mycoplasma pneumoniae IgM antibody test card |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106404754A (en) * | 2016-06-30 | 2017-02-15 | 深圳市亚辉龙生物科技股份有限公司 | A chlamydiae pneumoniae IgM chemiluminescent immunoassay kit and a preparing method thereof |
| CN106525836A (en) * | 2016-12-07 | 2017-03-22 | 无锡艾科瑞思产品设计与研究有限公司 | Detection method and detection card for macrodantin metabolite in milk products |
| CN110133264A (en) * | 2019-06-11 | 2019-08-16 | 上海雄图生物科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed |
-
2014
- 2014-12-18 CN CN201420811000.5U patent/CN204405675U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106404754A (en) * | 2016-06-30 | 2017-02-15 | 深圳市亚辉龙生物科技股份有限公司 | A chlamydiae pneumoniae IgM chemiluminescent immunoassay kit and a preparing method thereof |
| CN106525836A (en) * | 2016-12-07 | 2017-03-22 | 无锡艾科瑞思产品设计与研究有限公司 | Detection method and detection card for macrodantin metabolite in milk products |
| CN110133264A (en) * | 2019-06-11 | 2019-08-16 | 上海雄图生物科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed |
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