CN201489004U - Card for testing gold mark of double labeling object for nasopharyngeal carcinoma - Google Patents
Card for testing gold mark of double labeling object for nasopharyngeal carcinoma Download PDFInfo
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- CN201489004U CN201489004U CN2009202224336U CN200920222433U CN201489004U CN 201489004 U CN201489004 U CN 201489004U CN 2009202224336 U CN2009202224336 U CN 2009202224336U CN 200920222433 U CN200920222433 U CN 200920222433U CN 201489004 U CN201489004 U CN 201489004U
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Abstract
The utility model provides a card for testing the gold mark of a double labeling object for nasopharyngeal carcinoma, and belongs to the field of medical diagnostic equipment. The card comprises a base card and a face card which can be doubled up; a sampling zone, a reagent reaction zone and a water absorption zone of the base card are sequentially arranged on a reaction surface of the base card from left to right; and a left water absorption zone, a window zone, a gold mark antibody zone and a right water absorption zone are sequentially arranged on a reaction surface of the face card from left to right. The utility model is characterized in that detection lines are arranged on the reagent reaction zone; detection lines are coated with nasopharyngeal carcinoma mark antigenic protein; the window zone corresponds to the reagent reaction zone of the base card; and the gold mark antibody zone corresponds to one area of the water absorption zone of the base card, which is close to one end of the reagent reaction zone. The card is made based on the immunity zone analysis technology, has the advantages of good specificity and sensitivity, easy operation, fast and convenient detection, wide market space and convenience for popularization and application, and is particularly suitable for large-scale screening.
Description
Technical field
The utility model relates to the nasopharyngeal carcinoma detectable, particularly a kind of nasopharyngeal carcinoma double-tagging thing gold label test card.
Background technology
(nasopharyngeal carcinoma NPC) is the cancer that occurs in the pharynx nasalis cell to nasopharyngeal carcinoma, and its pathology is run initially in the mucous membrane of nasopharynx epithelium, upwards can invade and basis cranii, can be transferred to cervical lymph node downwards, and the multiple age is between 35-50 year.According to World Health Organization's statistics, the population that the whole world is subjected to nasopharyngeal carcinoma morbidity threat surpasses 400,000,000, but concentrates on southern china, Taiwan, Hong Kong and south east asia above 80% nasopharyngeal carcinoma patient.Present case is Most patients late period when being made a definite diagnosis, even through treatment, five year survival rate only is below 20%.If the energy early diagnosis is in time treated, then 5 years survival rates can be brought up to more than 80%.As seen diagnosis of nasopharyngeal carcinoma is the main approach that the patient obtains long term survival.
The test card of the detection various diseases that the golden labeling antibody of a lot of employings makes is arranged at present, and it is portable, simple to operate that test card has, even need not hospital and can carry out tentative diagnosis to self, also can be used for extensive examination disease.As the patent No. be, 200520006609.6. name is called " collaurum carcinoma of urinary bladder detectable bar " and has just adopted collaurum mark technology, detect the bacterium keratin in the urine, thereby the judgement carcinoma of urinary bladder, but reagent strip is collaurum, and antibody is fixed on the reagent strip, adopted simple immunity district to analyse technology, colloid golden watch antibody is analysed detection line to coated antibody with liquid to be measured district, and other foreign proteins in the liquid to be measured form serious noise for testing process, influence detection sensitivity and specificity.Early detection for nasopharyngeal carcinoma, the reagent strip series products that is not fit to early detection at present, because early detection is valued for susceptibility specificity height, and these test-strips change bag into by nasopharyngeal carcinoma labelled antigen or antibody at present, be difficult to reach the purpose of early detection, false negative is higher than regular meeting.When having discovered that some can be used for wrapping by the significant antibody of reagent strip or antigen, more require special test-strips to bring into play the measuring ability of these marks.
Experimental study and clinical practice for many years is verified, and the generation of ebv infection and nasopharyngeal carcinoma is closely related.Epstein-Barr virus is a kind of gamma herpes viruses, and this virus is carried near 95% adult in the whole world, and Epstein-Barr virus is activated and infects the pharynx nasalis cell, when entering the cracking replication status latent period, causes pharynx nasalis cell generation abnormal division hyperplasia to cause canceration.Have antibody repertoire among the nasopharyngeal carcinoma patients serum, generally assess the risk factor of suffering from nasopharyngeal carcinoma, for the diagnosis and the examination of nasopharyngeal carcinoma provides effective detection index by detecting serum Epstein-Barr virus antibody at multiple Epstein-Barr virus antigen.Because anti-VCA/IgG antibody early occurs at the Epstein-Barr virus acute infection period, can keep high level or rising all the life, thereby become the good index of Epstein-Barr virus acute infection or previous infection.Though the VCA/IgG antibody testing method is very sensitive to ebv infection, not special to nasopharyngeal carcinoma, because the high VCA-IgG that tires is also shown in monocytosis,mononucleosis, Burkitt ' s lymthoma, and immunosuppressant patient or the like.Rta is the encoding transcription activator protein of the immediate early gene BRLF1 that just expressed when just having entered the cracking replication status of Epstein-Barr virus, application number: in 200610113403 the patent, open Rta albumen is that Epstein-Barr virus enters essential active element of cracking replication status and only expression when nasopharyngeal carcinoma takes place.A large amount of clinical testings has shown that the anti-Rta/IgG antibody that detects in the blood has high specificity to the diagnosis nasopharyngeal carcinoma, and many indexs use in conjunction is tended in nasopharyngeal carcinoma serodiagnosis at present.Wuzhou, Guangxi Cai Yonglin of institute of oncology etc. once to Epstein-Barr virus capsid antigen (VCA), early antigen (EA), nuclear antigen (NA1) and cracking immediately the antibody various combination of 4 kinds of Epstein-Barr virus antigens such as early antigen Rta the diagnostic value of nasopharyngeal carcinoma was made the evaluation Journal of Sex Research, the result shows, single index, the susceptibility of VCA antibody is the highest, and all the other susceptibilitys of 3 are similar; And the specificity of Rta antibody is the highest, and the specificity of other 3 kinds of antibody is close.Passing judgment in the various index assembled schemes with logistic regression model associating ROC curve, find that antiviral capsid antigen VCA and the anti-antibody of early antigen Rta immediately have good complementation when the serodiagnosis nasopharyngeal carcinoma, thereby diagnosis usefulness is the highest in the combination of other pairs index, be to be the best of breed index, help to improve diagnosis efficiency.From the angle of cost-benefit, the immune globulin antibody of VCA and Rta should be the clinical serodiagnosis of nasopharyngeal carcinoma one of the most effective combination.
Though according to above-mentioned achievement in research, people can check in early days to nasopharyngeal carcinoma, but be difficult to break away from the interference of non-specific antibody in the test serum based on the detection that the enzyme linked immunoassay principle is advanced, and complex steps, to the reagent requirement strictness, detection efficiency is low, is not suitable for extensive high efficiency early screening work.
The utility model content
The utility model is according to the blank in above-mentioned field, provide a kind of based on colloid gold label machine and the oppositely immune nasopharyngeal carcinoma colloid gold label antibody test card of analysing technology and designing of distinguishing, characteristics with high specific and sensitivity, easy operating are suitable for the early stage nasopharyngeal carcinoma of efficient examination.
Nasopharyngeal carcinoma double-tagging thing gold label test card, but comprise the end card and the face card of doubling, be followed successively by sample application zone, reagent reacting district and end card suction zones on the reaction surface of card of the described end from left to right; Be followed successively by left suction zones on the reaction surface of described card from left to right, viewfinder area, Jin Biaokangtiqu and right suction zones, it is characterized in that, described reagent reacting district is provided with and is coated with nasopharyngeal carcinoma sign antigen protein on detection line, the described detection line, described viewfinder area is corresponding to the reagent reacting district of card of the described end, and described Jin Biaokangtiqu is corresponding to the zone of end card suction zones near reagent reacting district one end.
Described sample application zone cladding material is a glass fibre, and described reagent reacting district cladding material is a nitrocellulose filter, and described suction zones cladding material is a cotton starch plate.
Described golden labeling antibody district cladding material is the collaurum bond pad that is fixed with the anti-people's antibody of rabbit of collaurum particulate mark.
Described detection line left side also has bag by the nature controlling line of goat anti-rabbit antibody.
Described detection line right side also has visual mark line.
Include in the described glass fibre can with protein bound coloured dyestuff, described coloured dyestuff refers to 0.4% trypan blue.
Described nasopharyngeal carcinoma sign antigen protein refers to VCA antigen or Rta antigen.
Described nasopharyngeal carcinoma sign antigen protein refers to VCA antigen and Rta antigen, and described detection line comprises two, and the detection line bag in the left side is by VCA antigen, and the detection line bag in the right is by Rta antigen.
Described reagent reacting head of district 25mm, wide 4.5mm; Described application of sample head of district 10mm, wide 4.5mm; End card suction head of district 15mm, wide 4.5mm; Described form head of district 25mm, wide 3mm; Gold labeling antibody head of district 5mm, wide 4.5mm; Described left and right suction zones, long 10mm, wide 4.5mm.
Described card and end card reaction surface are with one side and link side by side.
The utility model nasopharyngeal carcinoma double-tagging thing gold label test card adopts the indirect immunochromatography technique of anti-stream, has got rid of the interference of non-specific antibody to test effectively.The right suction zones place chromatography that earlier damping fluid (PBS) is added in the face card during mensuration redissolves golden labeling antibody to golden labeling antibody district people, the sample application zone that then test serum is added in end card is in district's effect of analysing of film and moves to right-hand member down, if there is in the test serum antibody that combines with the sign antigentic specificity to exist, then with the reagent reacting district on the nasopharyngeal carcinoma labelled antigen that is coated on the detection line combine the formation antigen antibody complex.Etc. serum to be measured by chromatography to reagent reacting district the right along the time, at end Ka Xishuiquchu with damping fluid, existing side by side soon, face card reaction surface is combined on the end card reaction surface, the powerful water sorption at suction zones place, a face card left side makes the liquid reversed flow in the reagent reacting district, non-specific antibody and irrelevant thing are analysed to the sample application zone direction zones of inversions of end card in the test serum, the anti-human immunoglobulin(HIg) antibody of gold mark rabbit that comes from face Ka Jinbiaokangtiquchu combines with antigen antibody complex on the detection line subsequently, then can see that from viewfinder area detection line is the peony lines, then experimental result is positive; If do not contain specific antibody to be measured in the blood preparation, just can not form antigen-antibody complex at the detection line place, the anti-human immunoglobulin(HIg) antibody of gold mark rabbit then can not be fixed on detection line position on the film, so the peony lines do not occur at the detection line place, test findings is negative, and this test card has been got rid of the interference of non-specific antibody to test effectively.Since golden labeling antibody combine with antigen-antibody compound each on a card, treat just to react after antigen-antibody reaction is fully with basic antibody, improved the sensitivity of detection, and the design analysed of zones of inversions has been discharged the interference of non-specific antibody effectively.
The material that sample application zone of the present utility model, reagent reacting district, suction zones and golden labeling antibody district material therefor can meet with well known to those skilled in the art and these regional functions, the glass fibre that the utility model preferably adopts is as the sample application zone material, nitrocellulose filter is as reagent reacting district material, cotton paddle board is as the suction zones material, and Jin Biaokangtiqu adopts collaurum bond pad.
The utility model preferably is provided with a bag by the nature controlling line of goat anti-rabbit antibody in detection line left side, when golden labeling antibody direction chromatography during, should take on a red color at nature controlling line to nature controlling line, otherwise the inefficacy of explanation test card, nature controlling line can prevent the testing result false negative.
The utility model also preferentially is provided with a visual mark line on detection line the right, as reminding observer's test serum to be arrived the edge, the right in reagent reacting district by chromatography, needs immediately to drip phosphate buffer in the suction zones of end card.
The utility model preferably dissolves in glass fibre and protein bound coloured dyestuff, can be the chromatography situation that the observer easily observes liquid to be measured.
The utility model adopts the nasopharyngeal carcinoma sign antigen VCA antigen and/or the Rta antigen of research at present, and the antigen coated test card of VCA is used for early prevention, wraps by the test card of Rta antigen because early diagnosis.
The utility model further preferred two kinds of antigens wraps simultaneously by same test card, can be used for early diagnosis and patient to be measured is carried out patient's condition classification by testing result, being convenient to the patient takes further measures, for example, VCA detection of antigens line only on the test card, two detection lines are set, the detection line bag in the left side is by VCA antigen, the detection line bag in the right is by Rta antigen, make the utility model become the double-tagging test card, it is high-risk that the result who detects can be divided into nasopharyngeal carcinoma with detected crowd, middle danger and 3 subgroups of low danger, thus instruct the doctor to take the different measures of following up a case by regular visits to.Our test card with VCA/IgG as elementary examination thing, if testing result only VCA/IgG present the positive and then be considered as low danger.If VCA/IgG and Rta/IgG are risk factor and high-risk degree in being considered as of the positive, so both simplified working routine, reduced workload, and reach good sensitivity, improve the specificity that detects, the examination crowd can also be divided into different dangerous levels, improve the work efficiency of crowd's nasopharyngeal carcinoma examination greatly, practice has very big value to the nasopharyngeal carcinoma mass survey.
The utility model nasopharyngeal carcinoma double-tagging thing gold label test card is preferably made following specification: described reagent reacting head of district 25mm, wide 4.5mm; Described application of sample head of district 10mm, wide 4.5mm; End card suction head of district 15mm, wide 4.5mm; Described form head of district 25mm, wide 3mm; Gold labeling antibody head of district 5mm, wide 4.5mm; Described left and right suction zones, long 10mm, wide 4.5mm, portable, economy is attractive in appearance in one.
The end of the present utility model card can be two cards that separate with the face card, Yi Bian but also can make the doubling hinge card that links, when making the hinge card, after needs guaranteed that the reaction surface of end card and face card is superimposed, the left end of end card and face card was to left end, and right-hand member is to right-hand member.
Comprehensive, the utility model is used to check nasopharyngeal carcinoma, sensitivity and specificity are good, and be simple in structure, easy to use, detect quick and operating personnel do not need technical training, instrument and equipment that need not be special, stable reagent, be convenient to preserve, be " POCT (point of care test, POCT) " Perfected process is particularly suited for extensive examination, the market space is wide, and is easy to utilize.
Description of drawings
The face card reaction surface schematic top plan view of Figure 1A the utility model nasopharyngeal carcinoma double-tagging thing gold label test card.
The end card reaction surface schematic top plan view of Figure 1B the utility model nasopharyngeal carcinoma double-tagging thing gold label test card.
The face card sectional schematic diagram of Fig. 2 A the utility model nasopharyngeal carcinoma double-tagging thing gold label test card.
The end card sectional schematic diagram of Fig. 2 B the utility model nasopharyngeal carcinoma double-tagging thing gold label test card.
Wherein: 2-left side suction zones, 3-viewfinder area, 4-Jin Biaokangtiqu, the right suction zones of 5-, 6-sample application zone, 7-nature controlling line, 8-detection line, 9-reagent reacting district, 10-mark line, card suction zones at the bottom of the 11-.
Embodiment
Embodiment 1.
As Figure 1A, Figure 1B, shown in Fig. 2 A and Fig. 2 B, the utility model nasopharyngeal carcinoma double-tagging thing gold label test card, but comprise the end card and the face card of doubling, be followed successively by sample application zone 6, reagent reacting district 9 and end card suction zones 11 on the reaction surface of card of the described end from left to right; Be followed successively by left suction zones 2 on the reaction surface of described card from left to right, viewfinder area 3, golden labeling antibody district 4 and right suction zones 5, it is characterized in that, described reagent reacting district 9 is provided with and is coated with nasopharyngeal carcinoma sign antigen protein on detection line 8, the described detection line 8, described viewfinder area 3 is corresponding to the reagent reacting district 9 of card of the described end, and described golden labeling antibody district 4 is corresponding to the zone of end card suction zones 11 near reagent reacting district 9 one ends.The utility model adopts the indirect immunochromatography technique of anti-stream, has got rid of the interference of non-specific antibody to test effectively.Golden labeling antibody is redissolved in right suction zones 5 places and 4 places, golden labeling antibody district that earlier damping fluid (PBS) are added in the face card during mensuration, the sample application zone 6 that then test serum is added in end card is under the chromatography effect of film to the right, and bottom card suction zones 11 ends move, if there is in the test serum antibody to be measured to exist, then with in the reagent reacting district 9 is coated on nasopharyngeal carcinoma labelled antigen on the detection line 8 and combines and form antigen antibody complex.Treat test serum by chromatography to the right in reagent reacting district 9 along the time, 11 places add the PBS damping fluid in end card suction zones, existing side by side soon, face card reaction surface is combined on the end card reaction surface, the powerful water sorption at suction zones 2 places, a face card left side makes the liquid reversed flow in the reagent reacting district 9, non-specific antibody and irrelevant thing are to the reverse chromatography of sample application zone 6 directions of end card in the test serum, the anti-human immunoglobulin(HIg) antibody of gold mark rabbit that comes from the golden labeling antibody district 4 of face card combines with antigen antibody complex on the detection line 8 subsequently, then can see that from viewfinder area 3 detection line 8 is the peony lines, then experimental result is positive; If do not conform to specific antibody to be measured in the blood preparation, just can not form antigen-antibody complex at detection line 8 places, the anti-human immunoglobulin(HIg) antibody of gold mark rabbit then can not be fixed on detection line 8 positions on the film, so the peony lines do not occur at detection line 8 places, test findings is negative.
The material that sample application zone 6 of the present utility model, reagent reacting district 9, suction zones and golden labeling antibody district 4 material therefors can meet with well known to those skilled in the art and these regional functions, the utility model preferably adopts the material of glass fibre as sample application zone 6, nitrocellulose filter is as reagent reacting district 9 materials, cotton paddle board is as the material of suction zones on end card and the face card, and golden labeling antibody district 4 adopts collaurum bond pad.
The utility model preferably is provided with a bag by the nature controlling line 7 of goat anti-rabbit antibody in detection line 8 left sides, when the reverse chromatography of golden labeling antibody in golden labeling antibody district 4 during to nature controlling line 7, nature controlling line 7 should take on a red color, otherwise the inefficacy of explanation test card, nature controlling line 7 can prevent the testing result false negative.
The utility model also preferentially is provided with a visual mark line 10 on detection line 8 the right, as reminding observer's test serum to be arrived the right in reagent reacting district 9 by chromatography along also dripping phosphate buffers in end card suction zones 11.
The utility model preferably dissolves in the glass fibre of sample application zone 6 and protein bound coloured dyestuff, can make the observer easily observe the chromatography situation of test serum.
The utility model adopts the nasopharyngeal carcinoma sign antigen VCA antigen and/or the Rta antigen of research at present, and the antigen coated test card of VCA is used for early prevention, wraps by the test card of Rta antigen because early diagnosis.
The utility model further preferred two kinds of antigens wraps simultaneously by same test card, can be used for early prevention and diagnosis and patient to be measured is carried out patient's condition classification by testing result, being convenient to the patient takes further measures, two detection lines 8 are set, the detection line bag in the left side is by VCA antigen, and the detection line bag in the right is by Rta antigen.Make the utility model become the double-tagging test card, the result of detection can be divided into detected crowd high-risk, the middle danger of nasopharyngeal carcinoma and 3 subgroups of low danger, thereby instructs the doctor to take the different measures of following up a case by regular visits to.Our test card with VCA/IgG as elementary examination thing, if testing result only VCA/IgG present the positive and then be considered as low danger.If VCA/IgG and Rta/IgG are risk factor and high-risk degree in being considered as of the positive.So both simplify working routine, reduced workload, and reached good sensitivity, improve the specificity that detects, the examination crowd can also be divided into different dangerous levels, improve the work efficiency of crowd's nasopharyngeal carcinoma examination greatly, practice has very big value to the nasopharyngeal carcinoma mass survey.
The utility model nasopharyngeal carcinoma double-tagging thing gold label test card is preferably made following specification: described reagent reacting district 9 long 25mm, wide 4.5mm; Described sample application zone 6 long 10mm, wide 4.5mm; End card suction zones 11 long 15mm, wide 4.5mm; Described viewfinder area 3 long 25mm, wide 3mm; Gold labeling antibody district 4 long 5mm, wide 4.5mm; Described left and right suction zones 2,5 long respectively 10mm, wide 4.5mm.Portable, economy is attractive in appearance in one.
The end of the present utility model card can be two cards that separate with the face card, Yi Bian but also can make the doubling hinge card that links, when making the hinge card, after needs guaranteed that the reaction surface of end card and face card is superimposed, the left end of end card and face card was to left end, and right-hand member is to right-hand member.
Use of the present utility model:
Before the use, test card and test serum all need return to room temperature, keep flat desktop.Earlier damping fluid (PBS) being added in the right suction zones 5 of face card and the anti-human immunoglobulin(HIg) antibody of rabbit that 4 places, golden labeling antibody district make the gold grain mark during mensuration redissolves, then the test serum sample is added in sample application zone 6 places of end card, albumen in its serum is also moved to end card suction zones 11 ends under the chromatography effect of film with the combination of trypan blue dyestuff, if there is in the serum antibody VCA/IgG to be measured to exist, then combines and form antigen antibody complex-1 with the VCA antigen of detection line 8 places, left side bag quilt on the nitrocellulose filter.If when having antibody Rta/IgG to be measured to exist in the sample, just with nitrocellulose filter on the Rta antigen of detection line 8 places, the right bag quilt combine formation antigen antibody complex-2; When treating that the trypan blue dyestuff is drawn to mark line 10 places, at end card suction zones 11 places with damping fluid PBS, existing side by side soon, the face card covers on the end card, the sample application zone 6 of maintenance end card and the left suction zones 2 of face card are at one end, the powerful water sorption at suction zones 2 places, a left side makes the liquid reversed flow on the nitrocellulose filter, non-specific antibody and irrelevant thing are to the reverse chromatography in sample application zone 6 places in the serum, the 4 anti-human immunoglobulin(HIg) antibody of gold mark rabbit that come combine with antigen antibody complex-1 and-2 from golden labeling antibody district subsequently, at two detection line places the peony lines appear respectively, the excessive anti-human immunoglobulin(HIg) antibody of gold mark rabbit chromatography wine-colored nature controlling line 7 occurs to nature controlling line 7 places and goat anti-rabbit immunoglobulin antibodies.If do not contain specific antibody to be measured in the serum specimen, just can not form antigen-antibody complex at detection line 8 places, the anti-human immunoglobulin(HIg) antibody of gold mark rabbit then can not be fixed on the detection line 8, so the peony lines do not appear in 8 places at detection line, test findings is negative, and the peony lines still appear in nature controlling line 7.
Concrete steps are as follows:
(1) 200 μ L damping fluids (PBS) are added in right suction zones 5 and 4 places, golden labeling antibody district,
(2) add sample blood, serum or a blood plasma (25-50ul) to sample application zone 6 places,
(3) then add 50 μ L damping fluids (PBS), pick up counting at sample application zone 6 places,
When (4) observing liquid to be measured above mark line 10, close end card and face card,
Read test findings in (5) 5 minutes, aubergine is taken out of existing, and test result should be read in the time of 5-10 minute, and it is invalid to judge after 20 minutes,
(6) this testing result reference:
Positive (+) two peonys are taken out of existing, and one is positioned at right side detection line 8, and another is positioned at nature controlling line 7, and testing result shows: contain Rta/IgG antibody in the sample, level is higher than normal value.
Claims (10)
1. nasopharyngeal carcinoma double-tagging thing gold label test card, but comprise the end card and the face card of doubling, be followed successively by sample application zone, reagent reacting district and end card suction zones on the reaction surface of card of the described end from left to right; Be followed successively by left suction zones on the reaction surface of described card from left to right, viewfinder area, Jin Biaokangtiqu and right suction zones, it is characterized in that, described reagent reacting district is provided with and is coated with nasopharyngeal carcinoma sign antigen protein on detection line, the described detection line, described viewfinder area is corresponding to the reagent reacting district of card of the described end, and described Jin Biaokangtiqu is corresponding to the zone of end card suction zones near reagent reacting district one end.
2. nasopharyngeal carcinoma double-tagging thing gold label test card according to claim 1 is characterized in that described sample application zone cladding material is a glass fibre, and described reagent reacting district cladding material is a nitrocellulose filter, and described suction zones cladding material is a cotton starch plate.
3. nasopharyngeal carcinoma double-tagging thing gold label test card according to claim 1 is characterized in that described golden labeling antibody district cladding material is the collaurum bond pad that is fixed with the anti-people's antibody of rabbit of collaurum particulate mark.
4. according to claim 1 or 2 or 3 described nasopharyngeal carcinoma double-tagging thing gold label test cards, it is characterized in that described detection line left side also is provided with the nature controlling line that is coated with goat anti-rabbit antibody.
5. according to claim 1 or 2 or 3 described nasopharyngeal carcinoma double-tagging thing gold label test cards, it is characterized in that described detection line right side also has visual mark line.
6. according to claim 2 or 3 described nasopharyngeal carcinoma double-tagging thing gold label test cards, it is characterized in that, include in the described glass fibre can with protein bound coloured dyestuff, described coloured dyestuff refers to 0.4% trypan blue.
7. according to claim 1 or 2 or 3 described nasopharyngeal carcinoma double-tagging thing gold label test cards, it is characterized in that: described nasopharyngeal carcinoma sign antigen protein refers to VCA antigen or Rta antigen.
8. according to claim 1 or 2 or 3 described nasopharyngeal carcinoma double-tagging thing gold label test cards, it is characterized in that described nasopharyngeal carcinoma sign antigen protein refers to VCA antigen and Rta antigen, described detection line comprises two, the detection line bag in the left side is by VCA antigen, and the detection line bag in the right is by Rta antigen.
9. according to claim 1 or 2 or 3 described nasopharyngeal carcinoma double-tagging thing gold label test cards, it is characterized in that: described reagent reacting head of district 25mm, wide 4.5mm; Described application of sample head of district 10mm, wide 4.5mm; End card suction head of district 15mm, wide 4.5mm; Described form head of district 25mm, wide 3mm; Gold labeling antibody head of district 5mm, wide 4.5mm; Described left and right suction zones, long 10mm, wide 4.5mm.
10. according to claim 1 or 2 or 3 described nasopharyngeal carcinoma double-tagging thing gold label test cards, it is characterized in that: described card and end card links side by side and reaction surface with the one side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202224336U CN201489004U (en) | 2009-09-07 | 2009-09-07 | Card for testing gold mark of double labeling object for nasopharyngeal carcinoma |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202224336U CN201489004U (en) | 2009-09-07 | 2009-09-07 | Card for testing gold mark of double labeling object for nasopharyngeal carcinoma |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201489004U true CN201489004U (en) | 2010-05-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009202224336U Expired - Lifetime CN201489004U (en) | 2009-09-07 | 2009-09-07 | Card for testing gold mark of double labeling object for nasopharyngeal carcinoma |
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| CN (1) | CN201489004U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103293319A (en) * | 2013-05-22 | 2013-09-11 | 苏州市马尔泰新材料有限公司 | Gold-labeled test paper for rapidly diagnosing carcinoma jaw |
| CN103308695A (en) * | 2013-05-22 | 2013-09-18 | 苏州市马尔泰新材料有限公司 | Cysteine-rich secretory protein 1 (CRISP1) kit for cheek carcinoma diagnosis |
| CN103308693A (en) * | 2013-05-22 | 2013-09-18 | 苏州市马尔泰新材料有限公司 | Application of secretory protein 1 rich in cysteine in medical apparatus |
| CN103308694A (en) * | 2013-05-22 | 2013-09-18 | 苏州市马尔泰新材料有限公司 | Gold-labeled kit for salivary gland carcinoma diagnosis |
-
2009
- 2009-09-07 CN CN2009202224336U patent/CN201489004U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103293319A (en) * | 2013-05-22 | 2013-09-11 | 苏州市马尔泰新材料有限公司 | Gold-labeled test paper for rapidly diagnosing carcinoma jaw |
| CN103308695A (en) * | 2013-05-22 | 2013-09-18 | 苏州市马尔泰新材料有限公司 | Cysteine-rich secretory protein 1 (CRISP1) kit for cheek carcinoma diagnosis |
| CN103308693A (en) * | 2013-05-22 | 2013-09-18 | 苏州市马尔泰新材料有限公司 | Application of secretory protein 1 rich in cysteine in medical apparatus |
| CN103308694A (en) * | 2013-05-22 | 2013-09-18 | 苏州市马尔泰新材料有限公司 | Gold-labeled kit for salivary gland carcinoma diagnosis |
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