CN1773260A - Method for measuring nitrate concentration and special reagent kit thereof - Google Patents
Method for measuring nitrate concentration and special reagent kit thereof Download PDFInfo
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- CN1773260A CN1773260A CN 200410090618 CN200410090618A CN1773260A CN 1773260 A CN1773260 A CN 1773260A CN 200410090618 CN200410090618 CN 200410090618 CN 200410090618 A CN200410090618 A CN 200410090618A CN 1773260 A CN1773260 A CN 1773260A
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Abstract
The present invention discloses a method for determining nitrate concentration and its special-purpose kit. Said kit includes reagent A and reagent B. The reagent A is basically formed from (by weight portion) 30-40 portions of citric acid, 4-6 portions of manganese sulfate, 1-3 portions of p-aminobenzene sulfonamide or aminobenzene sulfonic acid and 1-2 portions of N-1-naphthyl ethylenediamine hydrochloride, and the described reagent B basically includes (by weight portion) 30-40 portions of citric acid and 1 portion of zinc powder. The present invention also provides the concrete steps of said method for determining nitrate concentration by using said kit.
Description
Technical field
The present invention relates to a kind of method and dedicated kit thereof of measuring nitrate concentration.
Background technology
At present, the nitrate concentration assay method of laboratory routine has: Pbenoldisulfonic Acid colourimetry, ultraviolet spectrophotometry, flow injection analysis.The Pbenoldisulfonic Acid colourimetry is classical nitrate concentration assay method, but this method complicated operation, minute long (2-3 hour) now seldom adopt; Ultraviolet spectrophotometry, flow injection method are present laboratory nitrate concentration assay methods commonly used, but these two kinds of methods all need expensive instrument.Therefore, the nitrate assay method of laboratory routine be difficult to easy, carry out outdoor mensuration nitrate concentration fast.
Summary of the invention
The method and the dedicated kit thereof that the purpose of this invention is to provide a kind of simple and efficient, sensitive and accurate mensuration nitrate concentration.
The kit of mensuration nitrate concentration provided by the present invention comprises reagent A and reagent B, wherein, described reagent A is made up of the material of following portions by weight substantially: citric acid 30-40, manganese sulfate 4-6, P-aminobenzene-sulfonamide or sulfanilic acid 1-3, N-1-naphthyl ethylenediamine hydrochloride 1-2; Described reagent B is made up of the material of following portions by weight substantially: citric acid 30-40, zinc powder 1-2.
Using the method for this kit measurement nitrate concentration, is to add reagent A and reagent B in liquid to be measured respectively, and described reagent A is identical with the final concentration of described reagent B, is 5-30mg/ml; Shake up, measure light absorption value or check color range concentration in 540nm behind the 4-6min.
Wherein, the final concentration of described reagent A and reagent B is preferably 10mg/ml.
Adopt the inventive method can measure soil extract, described soil extract is that pedotheque be impregnated in CaSO
4Solution obtains.
Described CaSO
4Solution is preferably saturated CaSO
4Solution, described pedotheque and saturated CaSO
4The w/v of solution (grams per milliliter) is 1: 10.During mensuration,, can suitably dilute carrying out soil extract earlier, and then measure wherein nitrate concentration if the color is too dark in colour developing.
The inventive method also can be used for measuring the water nitrate concentration.During mensuration, can be earlier with pressing the juice pincers that plant tissue hydraulic pressure is gone into 2 of little plastic tubes (0.1ml), little plastic tube input is filled in the plastic bottle of 100ml pure water and dilutes, then sampling and measuring.During mensuration,, also can suitably dilute earlier and then measure if the color is too dark in colour developing.
The kit sensitivity of mensuration nitrate concentration provided by the present invention, accuracy height, the holding time is long, and the reagent B for being easy to take place reduction reaction also can preserve more than 3 months at least; Assay method provided by the present invention, the nitrate concentration scope that can detect is wide, NO
3 --N all can directly measure in the 0.1-2mg/kg scope; Each measurement reagent dosage is few, generally only needs about 0.2 gram, and cost of determination is low; Measurement result is accurate, compares with the determined by ultraviolet spectrophotometry value, all reaches the utmost point level of signifiance of linear dependence, and linearly dependent coefficient reaches more than 0.9000; Minute is short, and is easy and simple to handle, can finish in 5-10 minute, can be widely used in the field measuring of nitrate.
Embodiment
Embodiment 1, nitrate concentration are measured the preparation of kit
This kit comprises reagent A and reagent B.
The constituent of reagent A and proportioning are: citric acid (analyzing pure) 30 grams, manganese sulfate (analyzing pure) 6 grams, P-aminobenzene-sulfonamide (analyzing pure) 1 gram, N-1-naphthyl ethylenediamine hydrochloride (analyzing pure) 2 grams.Citric acid is earlier levigate, and other reagent adds, mixes, grinds the back one by one and crosses the 0.5mm sieve, and packing into, dry bottle is airtight to keep in Dark Place.
Reagent B is: citric acid (analyzing pure) 30 grams, zinc powder (analyzing pure) 2 grams.Citric acid is earlier levigate, adds, mixes, grinds the back behind the zinc powder and cross the 0.5mm sieve, and packing into, dry bottle is airtight to keep in Dark Place.
The preparation of embodiment 2, kit
Consisting of of reagent A: citric acid (analyzing pure) 40 grams, manganese sulfate (analyzing pure) 4 grams, sulfanilic acid (analyzing pure) 3 grams, N-1-naphthyl ethylenediamine hydrochloride (analyzing pure) 1 gram.
Reagent B consists of: citric acid (analyzing pure) 40 grams, zinc powder (analyzing pure) 1 gram.
The preparation method of reagent A and reagent B is with embodiment 1.
Nitrate content in embodiment 3, the application kit measurement soil of the present invention
When measuring Soil Nitrate, get fresh soil sample 5g in plastic bottle, add CaSO
4Saturated solution 50ml shook 5 minutes, was filled into small beaker, got 10ml filtrate in color comparison tube, added each 0.1 gram of embodiment 1 gained reagent A, reagent B respectively, shake immediately, after five minutes with spectrophotometer in 540nm place colorimetric, or check color range concentration.Measure again after can suitably diluting if the color is too dark.
It is as shown in table 1 to adopt the inventive method to measure with conventional determined by ultraviolet spectrophotometry result to Beijing area grainfield pedotheque nitrate, the result shows, inventive method measured value and determined by ultraviolet spectrophotometry value are very identical, regression equation is y=0.99x-0.1703 (x, y are respectively ultraviolet spectrophotometry value and the inventive method measured value), r=0.9895 reaches the utmost point level of signifiance of linear dependence.
Table 1. different soils sample adopts the comparison of the inventive method measured value and determined by ultraviolet spectrophotometry value
| The pedotheque numbering | Inventive method measured value (mg/kg) | Determined by ultraviolet spectrophotometry value (mg/kg) |
| 1 2 3 | 6.69 2.92 21.39 | 6.20 3.28 18.65 |
| 4 5 6 7 8 9 10 11 12 | 8.88 3.69 17.29 4.79 37.35 27.22 14.44 50.38 35.12 | 7.33 2.95 22.30 5.12 35.54 23.78 14.97 52.08 33.63 |
Nitrate content in embodiment 4, the application kit measurement plant of the present invention
When measuring plant nitrate, with pressing the juice pincers that vegetables petiole (or fruit) is pressed into 2 of little plastic tubes (0.1ml), then little plastic tube input is filled in the plastic bottle of 100ml pure water, get 10ml solution after shaking up in color comparison tube, add embodiment 2 gained reagent A, each 0.1 gram of B respectively, shake immediately, after five minutes with spectrophotometer in 540nm place colorimetric, or check color range concentration.Measure again after can suitably diluting if the color is too dark.
It is as shown in table 2 to adopt the inventive method to measure with conventional determined by ultraviolet spectrophotometry result to Beijing area vegetable sample nitrate, the result shows, inventive method measured value and determined by ultraviolet spectrophotometry value are coincide better, regression equation is y=1.0601x-294.31 (x, y are respectively ultraviolet spectrophotometry value and the inventive method measured value), r=0.9728 reaches the utmost point level of signifiance of linear dependence.
Table 2. different vegetable earth sample adopts the comparison of the inventive method measured value and determined by ultraviolet spectrophotometry value
| The vegetable sample numbering | Inventive method measured value (mg/kg) | Determined by ultraviolet spectrophotometry value (mg/kg) |
| 1 2 3 4 5 6 7 8 9 10 11 12 | 1934 1357 1241 2368 2553 1620 1491 1400 2400 1600 1400 2400 | 2128 1554 1603 2395 2570 1728 1670 1719 2713 1635 1604 2544 |
Claims (6)
1, a kind of kit of measuring nitrate concentration comprises reagent A and reagent B, wherein, described reagent A is made up of the material of following portions by weight substantially: citric acid 30-40, manganese sulfate 4-6, P-aminobenzene-sulfonamide or sulfanilic acid 1-3, N-1-naphthyl ethylenediamine hydrochloride 1-2; Described reagent B is made up of the material of following portions by weight substantially: citric acid 30-40, zinc powder 1-2.
2, application rights requires the method for 1 described kit measurement nitrate concentration, adds reagent A and reagent B in liquid to be measured respectively, and described reagent A is identical with the final concentration of described reagent B, is 5-30mg/ml; Shake up, measure light absorption value or check color range concentration in 540nm behind the 4-6min.
3, method according to claim 2 is characterized in that: the final concentration of described reagent A and described reagent B is 10mg/ml.
4, according to claim 2 or 3 described methods, it is characterized in that: described liquid to be measured is soil extract, and described soil extract is in CaSO with the pedotheque lixiviate
4Solution obtains.
5, method according to claim 4 is characterized in that: described CaSO
4Solution is saturated CaSO
4Solution; Described pedotheque and saturated CaSO
4The w/v grams per milliliter of solution is 1: 10.
6, according to claim 2 or 3 described methods, it is characterized in that: described liquid to be measured is water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410090618 CN1773260A (en) | 2004-11-09 | 2004-11-09 | Method for measuring nitrate concentration and special reagent kit thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410090618 CN1773260A (en) | 2004-11-09 | 2004-11-09 | Method for measuring nitrate concentration and special reagent kit thereof |
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| CN1773260A true CN1773260A (en) | 2006-05-17 |
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| CN 200410090618 Pending CN1773260A (en) | 2004-11-09 | 2004-11-09 | Method for measuring nitrate concentration and special reagent kit thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103534590A (en) * | 2011-03-15 | 2014-01-22 | 哈克兰格有限责任公司 | Method of determining a concentration of nitrate |
| CN106198425A (en) * | 2016-06-24 | 2016-12-07 | 中国林业科学研究院热带林业实验中心 | A kind of soil nitrate-N detection method |
| CN106353304A (en) * | 2016-08-10 | 2017-01-25 | 浙江大学 | Method and color card for rapid detection of nitrate in food and kit for detecting nitrate in food |
| CN107764815A (en) * | 2017-09-29 | 2018-03-06 | 郑州欧柯奇仪器制造有限公司 | The method of quick detection Nitrate Content in Food |
| CN109827921A (en) * | 2019-03-12 | 2019-05-31 | 闫宏涛 | A kind of NO3-N and NO2-N assay analoids |
-
2004
- 2004-11-09 CN CN 200410090618 patent/CN1773260A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103534590A (en) * | 2011-03-15 | 2014-01-22 | 哈克兰格有限责任公司 | Method of determining a concentration of nitrate |
| CN103534590B (en) * | 2011-03-15 | 2015-11-25 | 哈克兰格有限责任公司 | Measure the method for nitrate concentration |
| CN106198425A (en) * | 2016-06-24 | 2016-12-07 | 中国林业科学研究院热带林业实验中心 | A kind of soil nitrate-N detection method |
| CN106353304A (en) * | 2016-08-10 | 2017-01-25 | 浙江大学 | Method and color card for rapid detection of nitrate in food and kit for detecting nitrate in food |
| CN107764815A (en) * | 2017-09-29 | 2018-03-06 | 郑州欧柯奇仪器制造有限公司 | The method of quick detection Nitrate Content in Food |
| CN109827921A (en) * | 2019-03-12 | 2019-05-31 | 闫宏涛 | A kind of NO3-N and NO2-N assay analoids |
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