CN112362648A

CN112362648A - Integrated test paper and preparation process thereof

Info

Publication number: CN112362648A
Application number: CN202011236155.7A
Authority: CN
Inventors: 王忠堂
Original assignee: Accuragen Inc
Current assignee: Accuragen Inc
Priority date: 2020-10-30
Filing date: 2020-10-30
Publication date: 2021-02-12
Anticipated expiration: 2040-10-30
Also published as: CN112362648B; CN114002210A; CN114002209A

Abstract

An integrated test paper is composed of base plate, baffle array, reagent block lattice and graphic code, and features that the reagent blocks are separated by baffles, the reagent blocks or reagent are loaded in special pen core, which is composed according to planar layout.

Description

Integrated test paper and preparation process thereof

Technical Field

The invention relates to the technical field of medical detection, in particular to integrated detection test paper and a preparation process thereof.

Background

The test paper is used as a classical solid-phase detection technology for detecting human body and natural samples, implementing component analysis and partially replacing a complex and expensive liquid-phase or gas-phase detection technology.

Based on the consideration of simultaneously satisfying the reading results of instruments and naked eyes, the reagent blocks of the test paper are generally designed to have larger sizes, the number of the reagent blocks contained in one test paper is limited, and meanwhile, based on the consideration of avoiding the interference among different reagent blocks, the reagent block spacing is designed to be larger, and the number of the reagent blocks contained in one test paper is more limited. If a plurality of indexes are detected, a large number of test paper and a large number of samples are needed, so that reagents and samples are wasted, and therefore, the application and popularization of test paper detection are limited.

In hospital blood examination, the total number of biochemical blood indicators, immunological indicators and cancer indicators is as high as hundreds, and a large amount of blood samples need to be repeatedly extracted, and a plurality of complex and expensive detection devices are used, so that a large amount of medical resources are consumed. If hundreds of blood biochemical indexes, immunity indexes and cancer indexes are detected on one piece of test paper containing hundreds of micro detection reagent blocks, a small amount of blood samples are extracted, hundreds of index detection results can be obtained through one-time detection, the blood detection efficiency of hospitals is improved, and the waste of medical resources is reduced.

In view of this, we provide an integrated test paper, which is prepared by a printing process, wherein hundreds of reagent blocks are arranged on one test paper, each reagent block detects one or more indexes, physical partitions are arranged among the reagent blocks, so that mutual interference among the reagent blocks is avoided, and the test result is accurately interpreted by means of computer vision and a specific algorithm.

Disclosure of Invention

The invention aims to provide an integrated test paper and a preparation process thereof, wherein hundreds of reagent blocks are arranged on one test paper, each reagent block is used for detecting one or more indexes, a few samples are realized, hundreds of index detection results can be obtained by one-time detection, the medical service efficiency is improved, and the resource waste is reduced.

The invention adopts the following technical scheme:

the invention provides integrated test paper which comprises a substrate, a baffle plate array, a reagent block dot matrix and a graphic code, wherein the baffle plate array is arranged on the substrate; the base plate is a reagent block lattice and a graphic code carrier and comprises a front side and a back side, and the front side is provided with a baffle plate array, a reagent block lattice and a graphic code; the baffles are arranged on the front surface of the substrate and are arranged in an array, the thickness of each baffle is not more than 0.5mm, the height of each baffle is not less than the thickness of a reagent block, the length and the width of each baffle depend on the size of the contained reagent block, and the baffles are used for partitioning and surrounding the reagent blocks so as to avoid the contact of reagent components among the reagent blocks, reagent reaction products and detection samples and reagents from overflowing; the reagent block is arranged on the front surface of the substrate and distributed in the array space of the baffle in a lattice manner, the types of the reagent block include but are not limited to dry chemical detection test paper, immunological detection test paper and chip test paper, the structure of the reagent block at least comprises one of a sample pad, a reagent combination pad, a reaction pad, a water absorption pad, a back plate and a protective film, and the detection reagent combination comprises a single item detection reagent combination and a multi item detection reagent combination; the graphic code is arranged at the blank end of the substrate, the types of the graphic code include but are not limited to a bar code, a two-dimensional code, a three-dimensional code and a chip, the graphic code bears basic information corresponding to the integrated test paper, and the basic information includes test paper production date, quality guarantee period, types and detection item data of each detection site.

Preferably, the chip test strip includes, but is not limited to, gene chip, protein chip, biochip, and chemical chip.

Preferably, the multi-item detection reagent combination integrates two or more item detection reagents into the same reagent block, different item detection substrates show different colors, or different item detection substrates show the same color, wherein different item detection substrates show reagent blocks of the same color, migration and aggregation positions of components to be detected on the reagent blocks are different, so that the same reagent block can analyze multiple components, when different item detection substrates show different colors, the two or more colors are developed together, the original single color intensity is analyzed according to a three-primary-color principle and an algorithm, the developed color values of the different item detection substrates are obtained respectively, and qualitative or quantitative analysis is performed on the different item detection substrates.

Preferably, the detection site detection item data comprises detection principles, inspection methods, notes, detection conditions, blank controls, standard controls, and algorithm schemes under actual detection conditions of detection items.

The invention also provides a preparation process of the integrated test paper, which comprises the steps of substrate preparation, reagent block printing, drying, quality inspection and packaging;

the preparation method of the substrate comprises the steps of designing a plane layout according to a detection project scheme, 3D modeling, designing a 3D mold, opening the mold and manufacturing the substrate comprising the baffle array structure, wherein the layout comprises a blank end, a baffle array and a reagent block dot matrix;

wherein, the reagent preparation is according to the detection project setting scheme, and detection reagents are prepared, and the reagents comprise but are not limited to enzyme, reaction substrate, chromogenic luminescent substrate, buffer solution, labeled antigen, labeled antibody, probe and stabilizer; the preparation process of the reagent block comprises the steps of preparing a dry chemical detection reagent block, preparing an immunological detection reagent block and preparing a chip reagent block, wherein the process of preparing the dry chemical detection reagent block comprises the steps of preparing a large piece of test paper, soaking the large piece of test paper into an adsorption reagent, drying the large piece of test paper, cutting the large piece of test paper into small pieces of reagent blocks, and loading the reagent blocks into a solid pen core, so that a large number of solid pen cores containing the test paper blocks of different detection items are prepared, each solid pen core comprises a pen tube, a reagent block, a bayonet and a cushion pad, wherein the upper end of the solid pen core is provided with a reagent block outlet, the bayonet is connected with a base station, the cushion pad is arranged in the bayonet, the lower end of the cushion pad is connected with a propelling device, the propelling device pushes the reagent blocks in the solid pen core to the upper end of the cushion pad through the cushion pad, so that the, preparing a reaction pad by coating an antibody or an antigen of an object to be detected with a large nitrocellulose membrane, preparing a sample pad, a water absorption pad, a back plate and a protective film, cutting the sample pad, a combination pad, the reaction pad, the water absorption pad and the protective film into strips, splicing and combining the sample pad, the combination pad, the reaction pad and the water absorption pad in sequence, adhering the strips to the back plate, adhering the protective film, covering the combination pad, the reaction pad and the water absorption pad with the protective film, preparing strip-shaped combination test paper, cutting the strip-shaped combination test paper into reagent blocks, and filling the reagent blocks into solid pen cores, thus preparing a large number of solid pen cores with different detection items;

the reagent block printing comprises designing a plane layout, typesetting, gluing a substrate and printing to obtain an integrated test paper finished product, removing the integrated test paper finished product, transferring a new substrate to a base station, printing, and performing the above circulation operation to prepare the integrated test paper in batches; the plane layout diagram is a reagent block lattice arrangement combination scheme of the integrated detection test paper; the typesetting process flow comprises the steps of arranging a solid refill array on a base station according to a plane layout, fixing and connecting a propelling end of a propelling device and a solid refill cushion pad; the substrate gluing process flow comprises the steps of gluing a front baffle array of a substrate, then wiping the upper edge of the baffle and gluing, and reserving a dot matrix site of a reagent block for gluing; the printing process flow comprises the steps that a glued substrate is transferred to a base station, reagent block dot matrix sites on the front surface of the substrate correspond to solid refill arrays on the base station, a pushing device pushes a buffer pad, the buffer pad discharges reagent blocks from the solid refills, the reagent blocks correspond to the solid refill dot matrix sites on the base station one by one according to a plane layout, the reagent blocks are adhered to the corresponding reagent block dot matrix sites of the substrate, meanwhile, a pattern code is printed to a blank end of the substrate by adopting a silk-screen printing technology to manufacture complete integrated test paper, and different types of integrated test paper are manufactured by designing different plane layout diagrams, typesetting and printing; the buffer cushion is internally provided with a pressure sensor, the buffer cushion pushes the reagent block to the substrate in the process that the propelling device pushes the buffer cushion, the built-in pressure sensor monitors the resistance between the reagent block and the substrate in real time and feeds the resistance between the reagent block and the substrate back to the control device of the propelling device, so that the control device makes a decision in time and automatically controls the propelling device to operate.

Preferably, the reagent block is loaded into the solid pen core, and the cut reagent block is accurately loaded into the solid pen core by a micro-processing technology combined with a robot technology.

Preferably, the reaction pad is coated with antigens or antibodies of a plurality of analytes, and can be used for simultaneously detecting and analyzing a plurality of analytes by the reagent block.

Preferably, the preparation process of the integrated test paper further comprises the steps of cutting a large piece of test paper into small test paper blocks, loading the test paper blocks into the solid pen core, soaking the test paper blocks into the adsorption reagent, drying, and preparing a large number of solid pen cores for specific detection items for preparing the integrated test paper.

Preferably, the preparation process of the integrated test paper further comprises cutting a large piece of test paper into small test paper blocks, sticking the test paper blocks to the reagent block dot matrix sites of the substrate by adopting a printing process, manufacturing the substrate containing the test paper block dot matrix, arranging different types of reagents in the liquid pen cores arranged in the typesetting array, infiltrating and adsorbing specific types of reagents in the test paper blocks at the substrate test paper block dot matrix sites in an ink-jet mode in a one-to-one correspondence manner, drying, and manufacturing a large amount of integrated test paper.

Preferably, the reagent block printing process flow further comprises the steps of gluing the substrate for pretreatment, so that the substrate is glued and carries charges, carrying opposite charges on the reagent blocks in the solid pen core in an intelligent control mode during printing, enabling the reagent blocks to be separated from the solid pen core in a one-to-one correspondence mode, attaching the reagent blocks to reagent block sites on the substrate, and manufacturing a large amount of integrated test paper through the subsequent steps of appropriate pressurization, drying treatment and the like.

Preferably, the propelling device comprises the screw system, the screw system comprises propelling ends, a support plate, an intelligent control motor and slider complex and a screw upright post, wherein the propelling ends are arranged on the support plate in an array mode, the distance between the propelling ends on the support plate is adjustable to meet the typesetting requirement, the support plate is connected with the intelligent control motor and slider complex, the intelligent control motor and slider complex moves along the screw upright post, and the screw upright post is fixed on the base station to further control the moving direction and distance of the propelling ends; according to actual needs, the base station can be connected with one or more lead screw systems so as to meet the requirements of pushing reagent blocks in the solid pen core to different distances.

Preferably, the propulsion device further comprises a pneumatic propulsion system, the pneumatic propulsion system comprises an intelligent control gas compressor, a gas pipeline, an intelligent control gas valve and gas nozzles, high-pressure gas generated by the intelligent control gas compressor passes through the gas pipeline and branches thereof and reaches the gas nozzles arranged in an array, and each gas nozzle is provided with one intelligent control gas valve; when the intelligent control gas compressor is started, the intelligent control gas valve is opened, high-pressure gas enters a bayonet at the lower end of the solid refill through the gas nozzle, the gas pressure pushes reagent blocks in the solid refill to be discharged from an outlet of the solid refill, the reagent blocks are pasted on a dot matrix site of the reagent blocks of the substrate, the pressure sensor in the buffer cushion senses that resistance between the reagent blocks and the substrate reaches a preset value, the intelligent control gas valve is closed, and the intelligent control gas compressor is stopped to obtain integrated detection test paper.

Preferably, the quality inspection comprises the steps of identifying whether the reagent block of the integrated test paper falls off or not, missing the reagent block and missing the graphic code by computer vision, and sampling the accuracy and the sensitivity of the detection result of the batch inspection integrated test paper.

Compared with the prior art, the invention has the beneficial effects that:

(1) the detection test paper is highly integrated, and high-efficiency multi-item detection is implemented

The test paper in the prior art is based on the consideration that the test paper can be simultaneously interpreted by an instrument and naked eyes, and the interference between reagents in different reagent blocks and the overflow of a sample are avoided, the size and the distance between the reagent blocks of the designed test paper are large, and the number of the reagent blocks arranged on one test paper is severely limited; in addition, in the prior art, reagent blocks with different reaction principles and reaction conditions are integrated in the same test paper, which leads to conflict of detection technical schemes.

The test paper of the invention has the advantages that the baffle array is arranged on the front surface of the substrate, different reagent blocks are separated by the baffle, and the height of the baffle is slightly higher than the thickness of the reagent blocks, so that the interference between the reagents in different reagent blocks and the overflow of a sample are avoided, the distance between the reagent blocks is reduced to a micron level, in addition, different reagent block lattice areas are arranged on one piece of test paper, the integration of the reagent blocks with different reaction principles and reaction conditions on the same piece of test paper becomes possible, hundreds of reagent blocks are arranged on the same piece of test paper, and hundreds of indexes are simultaneously detected. By means of micro-processing technology, robot technology and computer vision micro-recognition result interpretation technology, the sizes of the reagent blocks and the graphic codes are reduced to millimeter and micron levels, hundreds of reagent blocks are arranged on one piece of test paper with common size, and the number of detection indexes of the same piece of test paper is greatly increased.

(2) The accurate and efficient preparation of the integrated test paper by typesetting and printing processes

The size of a reagent block of the test paper in the prior art is large, and the reagent block can be pasted on a test paper substrate by means of a manual scheme or a robot scheme.

The test paper is prepared by adopting millimeter-sized and micron-sized reagent blocks, the special pen cores filled with the reagent blocks or reagents are typeset according to a pre-designed plane layout diagram only by means of a robot scheme, the reagent blocks or the reagents in the special pen cores are printed on a substrate by means of a charge force, mechanical or pneumatic propulsion device, the reagent blocks or the reagents are equivalent to printing ink of a printing art, the special pen cores are equivalent to single lettering boards of the printing art, the special pen cores are equivalent to a movable letter master board collected by the single lettering boards of the printing art, the substrate is equivalent to paper of the printing art, and the reagent blocks or the reagents in different types and different forms are efficiently and accurately pasted or sprayed to corresponding point positions of the test paper substrate to form a reagent block dot matrix on the substrate.

(3) The pen core is manufactured by two technical schemes, adopts delicate design, meets the requirements of different printing processes, and is used for preparing integrated detection test paper

One is as follows: the solid refill comprises a refill tube, reagent blocks, a buffer cushion and bayonets, wherein each solid refill is provided with one reagent block, the upper end of each solid refill is provided with a reagent block outlet, the bayonets at the lower end of each solid refill are connected with a base station, a propelling device adopts a screw system, an electric charge system or a pneumatic system, intelligent control is realized, the reagent blocks in the solid refills are pushed towards the outlets, the reagent blocks at the uppermost part in the solid refills are pasted to a base plate, integrated detection test paper is prepared, a pressure sensor in the buffer cushion collects pressure data between the reagent blocks and the base plate, and pressure data information is fed back to the propelling.

The second is that: the liquid pen core comprises a pen tube, reagents, an intelligent pump and a touch nozzle, wherein each liquid pen core is filled with a detection reagent, one end of each liquid pen core is a detection reagent nozzle and is connected with an intelligent control pump device, the reagents in the liquid pen cores are printed on test paper blocks of corresponding reagent block lattice sites on a substrate in an ink-jet mode, the amount of the reagents sprayed is accurately controlled, and integrated detection test paper is prepared.

Drawings

In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without inventive labor. Furthermore, these descriptions should not be construed as limiting the embodiments.

FIG. 1 is a schematic diagram of an integrated test strip substrate and a baffle array structure;

FIG. 2 is a schematic view of a baffle unit construction;

FIG. 3 is a schematic structural diagram of the integrated test paper including a substrate, a baffle array, a reagent block lattice, and a graphic code;

FIG. 4 is a schematic view of an integrated test paper structure with a baffle array and a reagent block lattice arranged in two regions;

FIG. 5 is a schematic view of an integrated test paper structure with three zones provided with a baffle array and a reagent block lattice;

FIG. 6 is a schematic diagram of the flow of the reagent block for immunological detection and the structure thereof;

FIG. 7 is a schematic sectional view of the reagent block for immunological detection;

FIG. 8 is a schematic diagram of a manufacturing process and structure of a solid refill;

FIG. 9 is a schematic diagram of a typesetting structure of the solid pen refill at a base platform;

FIG. 10 is a schematic diagram of a structure of an integrated test paper printed on a solid refill;

FIG. 11 is a schematic diagram of a liquid cartridge filled with a reagent;

FIG. 12 is a schematic view of a structure of an integrated test paper printed on a liquid refill;

FIG. 13 is a flow chart of the process for preparing the integrated test strip;

FIG. 14 is a flow chart of a substrate preparation process;

FIG. 15 is a flow chart of a dry chemistry test reagent block manufacturing process;

FIG. 16 is a flow chart of a manufacturing process of an immunological detection reagent block;

FIG. 17 is a process flow chart of the solid refill typesetting, printing and manufacturing integrated test paper;

FIG. 18 is a flow chart of the process of printing and manufacturing integrated test paper for solid refill;

FIG. 19 is a flow chart of other solid refill manufacturing processes;

FIG. 20 is a flow chart of the process for preparing typesetting printing integrated test paper for liquid pen refills.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, the present invention is further illustrated below with reference to specific examples. It is to be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention, which is to be given the full breadth of the appended claims and any and all equivalent modifications thereof which may occur to those skilled in the art upon reading the present specification.

It should be noted that the terms of directions such as up, down, left, right, front, back, side, etc. in the present embodiment are only relative concepts or reference to the normal use state of the product, and should not be considered as limiting.

As shown in FIGS. 1-20, the embodiment of the present invention discloses an integrated test strip and a process for preparing the same.

Referring to fig. 1, 2 and 3, the integrated test strip includes a substrate 1, a baffle 2 array, a reagent block 4 dot matrix and a graphic code 3. In order to show the structural key points of the embodiment of the invention simply and clearly, in the illustration, the array and the dot matrix of the baffle 2 array and the dot matrix of the reagent block 4 are exemplified by an array and a dot matrix of three rows and five columns, in fact, the number of rows and the number of columns of the array and the dot matrix of the reagent block 4 of the baffle 2 array can be set to be several, dozens or hundreds according to the requirement, and one master graphic code 3 and three auxiliary graphic codes 3-1 are exemplified, in fact, a plurality of master graphic codes 3 and auxiliary graphic codes 3-1 can be set according to the requirement.

The substrate 1 comprises a front surface 1-4 and a back surface 1-3, the front surface 1-4 comprises a blank end 1-1 and a testing end 1-2, the blank end 1-1 is provided with a main graphic code 3 and an auxiliary graphic code 3-1, the testing end 1-2 is provided with a baffle 2 array and a reagent block 4 dot matrix, and the back surface 1-3 is smooth and flat. The graphic code 3 and the auxiliary graphic code 3-1 are arranged on the blank end 1-1 of the substrate 1, and also can be arranged on the upper edge of the wall 2-1-1 of the baffle 2 or on the edge of the testing end 1-2, and the types of the graphic codes comprise bar codes, two-dimensional codes, three-dimensional codes and chips, and bear basic information corresponding to the integrated test paper.

The baffle 2 is arranged on the front surface 1-4 of the base plate 1, a plurality of baffle 2 units are arranged in a combined mode to form an array of baffles 2, the thickness of the wall 2-1-1 of the baffle 2 is not more than 0.5mm, the height of the wall 2-1-1 of the baffle 2 is not less than the thickness of the accommodated reagent block 4, the length and the width of the wall 2-1-1 of the baffle 2 depend on the size of the accommodated reagent block 4, and the area 2-1-2 surrounded by the wall 2-1-1 of the baffle 2 is used for accommodating the reagent block 4. The array of the baffle 2 and the lattice of the reagent block 4 form a one-to-one corresponding site relation which is used for dividing and surrounding the reagent block 4, and avoiding the contact of reagent components and reagent reaction products among the reagent blocks 4 and the overflow of a detection sample and a reagent.

Referring to fig. 4, a blank end 1-1 is arranged in the center of a front surface 1-4 of a substrate 1, test ends 1-2A and 1-2B are respectively arranged on two sides of the front surface 1-4 of the substrate 1, the blank end 1-1 is used for printing a main graphic code 3 and an auxiliary graphic code 3-1 of the test ends 1-2A and 1-2B, and the test ends 1-2A and 1-2B are respectively provided with a baffle 2 array and a reagent block 4 dot matrix for setting two types of detection items with large differences, such as dry chemical detection, immunological detection, chip detection and other detection, so that different intervention steps, such as heating, refrigeration, moisture preservation, drying, reagent addition, special light irradiation and special wavelength color collection and analysis, can be applied to two different partitions of the same piece of detection paper.

Referring to fig. 5, a front surface 1-4 of a substrate 1 is respectively provided with a testing end 1-2A, a blank end 1-1A, a testing end 1-2B, a blank end 1-1B, and a testing end 1-2C from left to right, the blank end 1-1A and the blank end 1-1B are used for printing a main graphic code 3 and an auxiliary graphic code 3-1 of the testing end 1-2A, the testing end 1-2B, and the testing end 1-2C are respectively provided with a baffle 2 array and a reagent block 4 array for setting three types of detection items with large differences, such as a dry chemical detection type, an immunological detection type, a chip detection type, and other detection types, which are beneficial to applying different intervention steps on three different partitions of the same piece of detection paper, such as heating, refrigeration, moisture preservation, drying, reagent addition, special light irradiation and special wavelength color collection and analysis.

By analogy, according to the types and characteristics of detection items, the partitions and the number of the test ends 1-2 and the blank ends 1-1 can be flexibly set on the front surface 1-4 of the substrate 1, and the partitions and the number of the arrays of the baffles 2 and the arrays of the reagent blocks 4 can be flexibly set, so that the detection requirements are met.

Referring to fig. 1, 3, 6 and 7, the reagent blocks 4 are disposed on the front surface 1-4 of the substrate 1 and are distributed in a lattice shape in the space of the area 2-1-2 surrounded by the array of the baffles 2. The types of the reagent block 4 include, but are not limited to, dry chemical detection reagent block, immunological detection reagent block, and chip reagent block, and the structure of the reagent block 4 at least includes one of a sample pad 10, a reagent combination pad 20, a reaction pad 30, a water absorption pad 40, a back plate 50, a protective film 60, and the like. The reagent block 4 comprises a reagent combination mode including single-item detection and multi-item detection, wherein a reagent substrate of the single-item detection shows a single color and is used for single component analysis and detection, a reagent of the multi-item detection comprises two or more items of detection reagents which are integrated into the same reagent block 4, different items of detection substrates show different colors, such as dry chemical detection test paper, more than two colors, the original single color intensity is analyzed according to the principle of three primary colors and an algorithm, specific component color development values are respectively obtained and are respectively subjected to qualitative or quantitative detection and analysis, or the substrates show the same color, such as immunological detection test paper and chip test paper, but migration distances or aggregation positions of components to be detected of different items on the reagent block 4 are different, so that the same reagent block 4 can analyze multiple components.

As shown in fig. 1-18, one embodiment of the present invention provides a process for preparing an integrated test strip, wherein the process 1000 comprises the following steps:

1100: preparing a substrate 1, wherein the preparation comprises the preparation of the substrate 1 and a baffle 2 array on the substrate 1;

1200: preparing a reagent, namely preparing a detection reagent and auxiliary reagents including but not limited to enzyme, reaction substrates, reaction intermediates, reaction primers, chromogenic luminescent substrates, buffer solution, labeled antigen, labeled antibody, nucleotide, polypeptide, DNA, RNA, probe and stabilizer according to a detection item setting scheme;

1300: preparing a reagent block 4, including but not limited to preparing a dry chemical detection reagent block 4, preparing an immunological detection reagent block 4 and preparing a chip reagent block 4, blending the reagent in the step 1200 into the test paper, preparing a small reagent block 4, and filling the reagent block 4 into the solid pen core 300;

1400: printing reagent blocks 4, namely typesetting the reagent blocks in the solid pen core 300 manufactured in the step 1300 on a base platform, transferring the reagent blocks to the substrate 1 prepared in the step 1100 in a printing mode, forming a reagent block 4 dot matrix in a baffle 2 array of the substrate 1, and simultaneously printing graphic codes 3;

1500: drying treatment, wherein the drying treatment comprises normal temperature drying treatment, low temperature drying treatment and vacuum low temperature drying treatment, so that the reagent block 4 is firmly adhered to the substrate 1 to obtain a finished product of the integrated test paper;

1600: quality inspection, which comprises detecting whether the reagent block 4 falls off or leaks from the dot matrix site of the reagent block 4 or not, detecting the loss of the graphic code 3 and detecting the accuracy, sensitivity and stability of the sampling batch inspection reagent block 4 by adopting computer vision identification, and providing a basis for improving the process flow;

1700: packaging, including single integrated test strip packaging to exclude air and moisture, and if necessary, enclosing a stabilizer or inert gas.

Referring to fig. 1 and 14, a substrate 1 preparation process 1100 includes the following steps:

1110: designing a plane layout according to the requirements of a detection project setting scheme, wherein the design of the plane layout comprises the positions and the number of blank ends 1-1, the positions and the number of detection ends 1-2 and the positions and the number of graphic codes 3, the appearance, the size and the arrangement mode of baffles 2 of a baffle 2 array and detection project type planning corresponding to corresponding reagent block 4 dot matrixes;

1120: according to the plan layout diagram of the step 1110, 3D modeling is carried out, and a 3D mold is designed, wherein the 3D mold comprises a substrate 1 and baffle 2 array integrated mold, a substrate 1 mold and a baffle 2 array mold;

1130: opening a mold according to the 3D modeling of step 1120;

1140: performing trial production on the mold, correcting the mold and shaping the mold;

1150: integrally molding a substrate 1 and a baffle 2 array integrated die to manufacture the substrate 1, wherein the substrate 1 is made of materials such as PVC, PE, carbon fiber, glass fiber and the like;

1160-1: manufacturing a substrate 1 by using a substrate 1 mould;

1160-2: manufacturing a baffle 2 array by using a baffle 2 array mould;

1170: bonding and combining the substrate 1 manufactured in the step 1160-1 and the baffle plate 2 array manufactured in the step 1160-2;

1180: or manufacturing the substrate 1 and the baffle 2 array by 3D printing according to the 3D modeling of the step 1120;

1190: step 1150, integrally forming, step 1170, adhering and combining the substrate 1 and the baffle 2 array, and step 1180, performing 3D printing on the substrate 1 and the baffle 2 array, and obtaining finished products of the substrate 1 and the baffle 2 array.

Referring to fig. 8, 9 and 15, the dry chemistry detection reagent block 4 manufacturing process 1310 includes the following steps:

1311: preparing a large piece of test paper and a reagent;

1312: soaking the large piece of test paper 200 in the step 1311 with an adsorption reagent;

1313: drying the large test paper 200 adsorbing the reagent in the step 1312, wherein the treatment modes comprise vacuum low-temperature drying and vacuum normal-temperature drying;

1314: cutting the dried large test paper 200 in the step 1313 into small reagent blocks 4;

1315: the reagent block 4 manufactured in the step 1314 is loaded into the solid refill 300, the solid refill 300 is manufactured by micromachining technology and robot technology, and comprises a pen tube 300-1, a reagent block 4, a cushion 300-2, a bayonet 300-3 and an outlet 300-4, the upper end of the solid refill is the outlet 300-4 of the reagent block 4, the lower end of the solid refill is the bayonet 300-3 connected with the base of the base table 400, the upper end of the bayonet 300-3 is the cushion 300-2, a pressure sensor is arranged in the cushion 300-2, the cushion 300-2 is connected with a propelling end 500-1 of a lead screw system 500 of the base table 400, the cushion 300-2 can move along the pen tube 300-1 within a certain damping range, the propelling end 500-1 of the lead screw system 500 pushes the cushion 300-2 to push the reagent block 4 in the solid refill 300 to the outlet 300-4, so that the uppermost reagent block 4 in the solid pen core 300 is discharged;

1316: the large piece of test paper 200 in step 1311 is soaked and adsorbed with different kinds of reagents to produce a large number of solid refills 300 containing test paper blocks 4 for different detection items.

Referring to fig. 6, 7, 8, 9 and 16, the process 1320 of manufacturing the immunological detection reagent block 4 includes the following steps:

1321: preparing a large sample pad 10, preparing a large binding pad 20 coated with a labeled antibody, a large absorbent pad 40, a large backboard 50 and a large protective film 60, wherein the labeled antibody is usually labeled by colloidal gold or is combined with a chromogenic substrate reagent by enzyme labeling, and the large protective film 60 is usually made of a water-repellent transparent material;

1322: preparing a large piece of nitrocellulose membrane to coat the antibody or antigen of the object to be detected, manufacturing a large piece of reaction pad 30, coating the large piece of reaction pad 30 with the antigen or antibody of various objects to be detected, and manufacturing a reagent block 4 for simultaneously detecting various objects to be detected, wherein the nitrocellulose membrane can also be replaced by other materials;

1323: cutting the large sample pad 10, the large bonding pad 20, the large reaction pad 30, the large absorbent pad 40, the large backboard 50 and the large protective film 60 obtained in the step 1321 into strip-shaped sample pads 10-1, bonding pads 20-1, reaction pads 30-1, absorbent pads 40-1, backboard 50-1 and protective film 60-1, wherein the width of the sample pads 10, the large bonding pads 20, the large reaction pads 30-1, the large backboard 50-1 and the protective film 60-1 is determined by the size of the reagent block 4 preset in the plan layout;

1324: splicing and combining the strip-shaped sample pad 10-1, the combination pad 20-1, the reaction pad 30-1 and the water absorption pad 40-1 which are manufactured in the step 1323 according to the plane sequence of the sample pad 10-1, the combination pad 20-1, the reaction pad 30-1 and the water absorption pad 40-1 from top to bottom, and adhering the spliced and combined sample pad, the combination pad, the reaction pad and the water absorption pad to the strip-shaped back plate 50-1 to manufacture a strip-shaped complex 600;

1325: the strip-shaped compound body 600 manufactured in the step 1324 is attached with a strip-shaped protective film 60-1, the protective film 60-1 covers the strip-shaped combination pad 20-1, the reaction pad 30-1 and the water absorption pad 40-1, and the strip-shaped sample pad 10-1 is kept in a naked state;

1326: obtaining different kinds of strip-shaped complex test paper 100 by coating antibodies or antigens of different kinds of objects to be tested;

1227: cutting the strip-shaped composite test paper 100 manufactured in the step 1326 into reagent blocks 4;

1228: filling the reagent block 4 prepared in the step 1327 into the solid pen refill 300;

1229: by changing the type of the labeled antibody coated by the 1321 large combined pad 20 and the type of the antibody or antigen of the substance to be detected coated by the 1322 large nitrocellulose membrane, a large number of solid pen cores 300 containing reagent blocks 4 with different detection items are manufactured.

Referring to fig. 9 and 17, reagent block printing 1400 includes the steps of:

1410: designing a reagent block 4 dot matrix plane layout diagram, a graphic code 3 plane layout diagram and a substrate 1 baffle 2 array plane diagram of the integrated test paper according to requirements;

1420: typesetting, namely arranging the solid pen cores 300 to a base station by referring to the reagent block 4 dot matrix plane layout diagram, the graphic code 3 plane layout diagram and the substrate 1 baffle 2 array plane diagram designed in the step 1410, and specifically comprising the following three sub-steps:

1420-1: preparing a solid pen core 300, and respectively filling different reagent blocks 4 into the solid pen core 300 for standby;

1420-2: preparing a base 400, and arranging the solid refills 300 prepared in the mounting step 1420-1 on the base 400 in an array;

1420-3: the solid pen core 300 prepared in the step 1420-1 is coupled to the propelling end 500-1 of the lead screw system 500 of the base 400 through the bayonet 300-3 at the lower end thereof; the lead screw system 500 comprises a pushing end 500-1, a supporting plate 500-2, an intelligent control motor and slider complex 500-3 and a lead screw upright 500-4, wherein the pushing end 500-1 is arranged on the supporting plate 500-2 in an array mode, the pushing end 500-1 can adjust the distance on the supporting plate 500-2 to meet the typesetting requirement, the supporting plate 500-2 is connected with the intelligent control motor and slider complex 500-3, the intelligent control motor and slider complex 500-3 moves along the lead screw upright 500-4, the lead screw upright 500-4 is fixed on the base 400, and further, the moving direction and distance of the pushing end 500-1 are controlled; a pressure sensor is arranged in a cushion pad 300-2 of the solid pen core 300 and used for sensing the resistance between the reagent block 4 and the substrate 1 and feeding back the resistance between the reagent block 4 and the substrate 1 to the intelligent control motor-slider complex 500-3, so that a controller of the intelligent control motor makes a decision timely and automatically controls the operation of the intelligent control motor-slider complex 500-3; according to actual needs, the base 400 can be connected with one or more lead screw systems 500 to meet the requirements of different propelling distances of the reagent blocks 4 in the solid pen core 300; in addition, the cushion 300-2 of the solid refill 300 can be additionally provided with a spring device so as to reduce the strict limitation requirement on the propelling precision of the screw rod system 500;

1430: gluing the substrate 1, namely gluing the glue on the testing end 1-2 of the front surface 1-4 of the substrate 1, then erasing the glue on the upper edge of the wall 2-1-1 of the baffle 2, and reserving the glue for containing the dot matrix sites of the reagent blocks 4;

1440: printing, namely conveying the glued substrate 1 prepared in the step 1430 to the base table 400 prepared in the step 1420, and adhering the reagent blocks 4 in the solid pen core 300 to the dot matrix sites of the reagent blocks 4 of the substrate 1 by virtue of a lead screw system 500 to manufacture integrated detection test paper;

1450: a series of solid pen refills 300 filled with different reagent blocks 4 are prepared by designing different reagent block 4 dot matrix plane layout diagrams, graphic code 3 plane layout diagrams and substrate 1 baffle array plane diagrams of the integrated test paper, combined typesetting is carried out, the reagent blocks 4 are printed on the substrate 1, different types of integrated test paper is obtained, and batch production is carried out.

Referring to fig. 9, 10 and 18, the printing process 1440 includes the following steps:

1441: the coated substrate 1 prepared in step 1430 is transferred to a base 400;

1442: accurately aligning the array of the solid pen cores 300 typeset by the base table 400 according to the plane layout diagram by the dot matrix sites of the reagent blocks 4 on the front surface 1-4 of the gluing substrate 1;

1443: the intelligent motor of the lead screw system 500 is started, the reagent block 4 in the solid pen core 300 is pushed to be discharged from the solid pen core 300 through the pushing end 500-1 and is attached to the dot matrix site of the reagent block 4 of the substrate 1, the pressure sensor arranged in the buffer pad 300-2 senses that the resistance between the reagent block 4 and the substrate 1 reaches a preset value, and the motor of the intelligent control motor-slider complex 500-3 stops;

1444: printing graphic codes to the blank end of the substrate according to a plane layout by adopting a silk-screen technology;

1445: removing the substrate 1 printed with the reagent block 4, receiving a new substrate 1 by the base table 400, performing the printing operation of the reagent block 4, and circulating the steps to prepare the integrated test paper in batches;

1446: designing different plane layout diagrams, typesetting and printing, and preparing different types of integrated test paper.

As shown in fig. 19, another embodiment of the present invention provides a process flow 2000 for manufacturing a solid pen core, comprising the following steps:

2100: preparing a large piece of test paper, a reagent and a blank solid refill 300;

2200: cutting a large piece of test paper into small test paper blocks;

2300: loading the test paper block produced in step 2200 into a blank solid refill 300;

2400: soaking the solid pen core 300 provided with the test paper block manufactured in the step 2300 in an adsorption reagent;

2500: drying the solid pen core 300 manufactured in the step 2400;

2600: manufacturing solid pen cores 300 for specific detection items in batches; according to the requirements, the reagent combination scheme is changed, and the solid pen core 300 with different detection items is manufactured.

Referring to fig. 11, 12 and 20, another embodiment of the present invention provides a process 3000 for preparing an integrated test strip, comprising the following steps:

3100: preparing a large piece of test paper 200, a substrate 1, a reagent and an empty liquid pen core 700; the liquid pen core 700 comprises a pen tube 700-1, a reagent 700-4, an intelligent pump 700-5 and a nozzle 700-6, wherein the intelligent pump 700-5 is started, and the reagent 700-4 contained in the pen tube 700-1 is sprayed out through the nozzle 700-6;

3200: cutting the large piece of test paper 200 into small test paper blocks;

3300: sticking the test paper block manufactured in the step 3200 to the reagent block 4 dot matrix sites of the baffle 2 array of the substrate 1 by adopting the process flow in the step 1440 to manufacture blank integrated test paper;

3400: filling different kinds of reagents into the empty liquid pen core 700 to manufacture the liquid pen core 700; the liquid cartridge 700 is laid out and fixed to the base 400 according to a plan layout previously designed;

3500: placing the blank integrated test paper manufactured in the step 3300 under a base table 400, enabling the reagent blocks 4 lattice sites on the front surface 1-4 of the test paper to be accurately aligned with the nozzles 700-6 of the liquid pen cores 700 arranged in the array, starting an intelligent pump 700-5, spraying the reagent 700-4 filled in the pen tube 700-1 to the test paper blocks on the reagent blocks 4 sites on the detection end 1-2 on the front surface 1-4 of the substrate 1 through the nozzles 700-6, infiltrating and adsorbing the specific type of reagent 700-4 by the test paper blocks, enabling the sprayed reagent to reach a preset dosage, and closing the intelligent pump 700-5;

3600: drying the integrated test paper soaked with the adsorption reagent prepared in the step 3500 to obtain integrated test paper;

3700: according to requirements, different types of reagents are filled in the empty liquid pen cores 700 to manufacture different types of liquid pen cores 700, and the different types of liquid pen cores 700 are arranged, combined and typeset for batch preparation of integrated test paper.

In addition, another embodiment of the present invention provides a reagent block printing process 4000 for replacing the lead screw system 500, comprising the following steps:

4100: pretreating the substrate 1 to enable the substrate 1 to be coated with glue and carry positive charges;

4200: the solid pen cores 300 are typeset and are arranged and fixed on the base platform 400 in an array mode, the pen tubes 300-1, the cushion pads 300-2 and the bayonets 300-3 of the solid pen cores 300 are made of insulating materials, the pen tubes 300-1 are internally provided with a conductive circuit, the base platform 400 is provided with a negative charge generating device, and the internally provided conductive circuit is connected with the negative charge generating device;

4300: transferring the substrate 1 which is manufactured in the step 4100 and carries the positive charge glue coating to a base table 400, so that the reagent blocks 4 lattice sites on the front surface 1-4 of the substrate 1 are accurately aligned with the outlets 300-4 of the solid pen cores 300 arranged in the array;

4400: the negative charge generating device is started, negative charges are transmitted to the reagent block 4 in the solid pen core 300 through the built-in conductive circuit, the reagent block 4 is separated from the solid pen core 300 under the action of charge force, and is adsorbed to the position of the reagent block 4 on the substrate 1 and is bonded, and the operation of printing the reagent block 4 on the substrate 1 is completed;

4500: stopping the operation of the negative charge generating device, discharging the residual reagent block 4 in the solid pen core 300, and removing the integrated detection test paper manufactured in the step 4400;

4600: the integrated test paper manufactured in step 4500 is subjected to appropriate pressurization and drying treatment to obtain a finished integrated test paper product. Of course, the substrate 1 may be pre-treated so that the glue carries negative charges, and accordingly, the base 400 is provided with a positive charge generating device.

In addition, another embodiment of the present invention provides a reagent block printing process 5000 for replacing the lead screw system 500, including the following steps:

5100: the glue-coated substrate 1 prepared in the step 1430 is transferred to a base table 400, the base table 400 is provided with a pneumatic system, the pneumatic system comprises an intelligent control gas compressor, a gas pipeline, an intelligent control gas valve and gas nozzles, high-pressure gas generated by the intelligent control gas compressor passes through the gas pipeline and branches thereof and reaches the gas nozzles arranged in an array, and each gas nozzle is provided with an intelligent control gas valve;

5200: accurately aligning the array of the solid pen cores 300 typeset by the base table 400 according to a plane layout diagram by using the dot matrix sites 4 of the reagent blocks 1-4 on the front surface of the gluing substrate 1, wherein an air seal is arranged in a bayonet 300-3 at the lower end of the solid pen core 300, and the air nozzle is inserted into the air seal;

5300: the intelligent control gas compressor is started, the intelligent control gas valve is opened, high-pressure gas enters a bayonet 300-3 at the lower end of the solid pen core 300 through a gas nozzle, the high-pressure gas pushes a reagent block 4 in the solid pen core 300 to be discharged from an outlet 300-4 of the solid pen core 300 and is adhered to a dot matrix site of the reagent block 4 of the substrate 1, a pressure sensor in the buffer pad 300-2 senses that resistance between the reagent block 4 and the substrate 1 reaches a preset value, the intelligent control gas valve is closed, and the intelligent control gas compressor stops;

5400: and removing the substrate 1 printed with the reagent blocks 4 to obtain the integrated test paper.

This application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. It is intended that the specification and examples be considered as exemplary only, with a scope of the invention being limited only by the following claims.

Claims

1. An integrated test paper, which is characterized by comprising: a substrate, a baffle plate array, a reagent block dot matrix and a graphic code,

the substrate comprises a front side and a back side, and the front side is provided with a baffle array, a reagent block lattice and a graphic code which are a reagent block lattice and a graphic code carrier;

the baffle is used for partitioning and surrounding the reagent blocks, so as to avoid the contact of reagent components and reagent reaction products among the reagent blocks and the overflow of a detection sample and a reagent, the array is arranged on the front side of the substrate, the height of the array is not less than the thickness of the reagent blocks, and the length and the width of the array are determined by the size of the accommodated reagent blocks;

the reagent blocks are arranged on the front surface of the substrate and distributed in the array space of the baffle in a lattice shape, the types of the reagent blocks comprise dry chemical detection test paper blocks, immunological detection test paper blocks and chip test paper blocks, the reagent blocks structurally comprise a sample pad, a reagent combination pad, a reaction pad, a water absorption pad, a back plate and a protective film, and the reagent combination comprises a single item detection reagent combination and a multi-item detection reagent combination;

the graphic code is arranged at the blank end of the substrate, the type of the graphic code comprises a bar code, a two-dimensional code, a three-dimensional code and a chip, and the graphic code is used for bearing basic information corresponding to the integrated test paper, and the basic information comprises the production date, the quality guarantee period, the type and the detection item data of each point of a reagent block dot matrix of the integrated test paper.

2. The integrated test strip of claim 1, wherein the chip test piece includes but is not limited to gene chip, protein chip, biochip, and chemical chip.

3. The integrated test strip of claim 1, wherein the multi-item test reagent combination is formed by integrating two or more items of test reagents into a same reagent block, and the test substrate displays comprise different color displays and a same color display, wherein the same color display is used for performing analytical test according to different migration and aggregation positions of the components to be tested on the reagent block, wherein the different color displays are used for performing common color development, and the original single color intensity is analyzed according to a three-primary-color principle and an algorithm, so as to respectively obtain the color development values of the test substrates of the specific components to be tested, and then the analytical test is performed.

4. The integrated test strip of claim 1, wherein the data of the site detection items includes, but is not limited to, detection principle, detection method, detection conditions, blank control, standard control, and algorithm under actual detection conditions of the detection items.

5. A preparation process of integrated test paper is characterized by comprising the following steps: preparing a substrate, preparing a reagent block, printing the reagent block, drying, inspecting quality and packaging;

the preparation of the substrate comprises designing a plane layout according to a detection project scheme, 3D modeling, designing a 3D mold, opening the mold, and manufacturing the substrate comprising the baffle array structure, wherein the plane layout comprises a blank end, a baffle array, a reagent block dot matrix and a graphic code;

the reagent preparation prepares detection reagents according to a detection project scheme, wherein the detection reagents comprise but are not limited to enzymes, reaction substrates, chromogenic luminescent substrates, buffer solutions, labeled antigens, labeled antibodies, probes and stabilizing agents;

the preparation of the reagent block comprises the preparation of a dry chemical detection reagent block, the preparation of an immunological detection reagent block and the preparation of a chip reagent block; the manufacturing process of the dry chemical detection reagent block comprises the steps of preparing a large piece of test paper, soaking the large piece of test paper with an adsorption reagent, drying the large piece of test paper, cutting the large piece of test paper into small pieces of reagent blocks, and loading the reagent blocks into a solid pen core, so that a large number of solid pen cores filled with test paper blocks of different detection items are manufactured, each solid pen core comprises a pen tube, a reagent block, a buffer cushion and a bayonet, the upper end of each solid pen core is provided with a reagent block outlet, the lower end of each solid pen core is provided with the bayonet and is used for being connected with a base station, the reagent block; the manufacturing process of the immunological detection reagent block comprises the steps of manufacturing a combination pad by coating a labeled antibody with a large piece of test paper, manufacturing a reaction pad by coating the antibody or antigen of an object to be detected with a large piece of nitrocellulose membrane, preparing a sample pad, a water absorption pad, a back plate and a protective film, cutting the sample pad, the combination pad, the reaction pad, the water absorption pad, the back plate and the protective film into strips, splicing and combining the sample pad, the combination pad, the reaction pad and the water absorption pad in sequence, sticking the sample pad, the water absorption pad, the back plate and the protective film, sticking the protective film on the sample pad, the reaction pad and the water absorption pad, manufacturing a strip-shaped test paper complex, further cutting the strip-shaped test paper complex into reagent blocks, and filling the reagent blocks;

the reagent block printing process flow comprises designing a plane layout, typesetting, coating glue on a substrate, printing to obtain an integrated test paper finished product, removing the integrated test paper finished product, transferring a new substrate to a base station, printing, and performing the circulation operation in such a way to prepare the integrated test paper in batches; the plane layout diagram is a reagent block lattice arrangement combination scheme of the integrated detection test paper; the typesetting process flow comprises the steps of arranging a solid refill array on a base station according to a plane layout, fixing and connecting a propelling end of a propelling device and a solid refill cushion pad; the substrate gluing process flow comprises the steps of gluing a front baffle array of a substrate, then wiping the upper edge of the baffle and gluing, and reserving a dot matrix site of a reagent block for gluing; the printing process flow comprises the steps that a glued substrate is transferred to a base station, reagent block dot matrix sites on the front surface of the substrate correspond to solid refill arrays on the base station, a pushing device pushes a buffer pad, the buffer pad discharges reagent blocks from the solid refills, the reagent blocks correspond to the solid refill dot matrix sites on the base station one by one according to a plane layout, the reagent blocks are adhered to the corresponding reagent block dot matrix sites of the substrate, meanwhile, a pattern code is printed to a blank end of the substrate by adopting a silk-screen printing technology to manufacture complete integrated test paper, and different types of integrated test paper are manufactured by designing different plane layout diagrams, typesetting and printing; the buffer cushion is internally provided with a pressure sensor, the buffer cushion pushes the reagent block to the substrate in the process that the propelling device pushes the buffer cushion, the built-in pressure sensor monitors the resistance between the reagent block and the substrate in real time and feeds the resistance between the reagent block and the substrate back to the control device of the propelling device, so that the control device makes a decision in time and automatically controls the propelling device to operate.

6. The process of claim 5, wherein the reagent block is loaded into the solid core by a micro-machining process in combination with robotics to accurately place the cut reagent block into the solid core.

7. The process of claim 5, wherein the reaction pad is coated with antigens or antibodies of a plurality of analytes, and the reagent patch is prepared for simultaneous detection and analysis of the plurality of analytes.

8. The preparation process of claim 5, further comprising cutting a large piece of test paper into small test paper blocks, loading the test paper blocks into a solid pen core, soaking the solid pen core test paper blocks with an adsorption reagent, and drying to prepare the solid pen core with specific detection items for preparing the integrated detection test paper.

9. The process of claim 5, further comprising cutting a large sheet of test paper into small test paper pieces, adhering the test paper pieces to a substrate by a printing process to produce a substrate comprising a matrix of the test paper pieces, arranging and fixing the reagent in the liquid cartridge on the base, impregnating the test paper pieces at the matrix sites of the test paper pieces on the substrate with the adsorbed reagent in a one-to-one correspondence by spraying, and drying to produce the integrated test paper.

10. The manufacturing process of claim 5, wherein the reagent block printing process further comprises pre-treating the substrate to make the substrate carry charges, and during printing, the reagent blocks in the solid pen core carry opposite charges in an intelligent control manner to promote the reagent blocks to be separated from the solid pen core under the driving of the charge force and to be attached to the substrate reagent block matrix sites in a one-to-one correspondence manner.

11. The preparation process according to claim 5, wherein the propulsion device comprises the screw system, the screw system comprises a propulsion end, a support plate, an intelligent control motor and slider complex and a screw upright post, wherein the propulsion end is arranged on the support plate in an array manner, the distance between the propulsion end and the support plate is adjustable to meet the typesetting requirement, the support plate is connected with the intelligent control motor and slider complex, the intelligent control motor and slider complex moves along the screw upright post, and the screw upright post is fixed on the base station to further control the moving direction and distance of the propulsion end; according to actual needs, the base station can be connected with one or more lead screw systems so as to meet the requirements of pushing reagent blocks in the solid pen core to different distances.

12. The process according to claim 5, wherein the propulsion device further comprises a pneumatic propulsion system, the pneumatic propulsion system comprises an intelligent control gas compressor, a gas pipeline, an intelligent control gas valve and gas nozzles, the high-pressure gas generated by the intelligent control gas compressor is led to the gas nozzles arranged in the array through the gas pipeline and the branches thereof, and each gas nozzle is provided with one intelligent control gas valve; when the intelligent control gas compressor is started, the intelligent control gas valve is opened, high-pressure gas enters the lower end bayonet of the solid refill through the gas nozzle, the high-pressure gas pushes the reagent blocks in the solid refill to be discharged from the outlet of the solid refill, the reagent blocks are pasted on the dot matrix sites of the reagent blocks of the base plate, the pressure sensor in the cushion pad senses that the resistance between the reagent blocks and the base plate reaches a preset value, the intelligent control gas valve is closed, and the intelligent control gas compressor is stopped to obtain the integrated detection test paper.