CN201522495U - A, B, and O blood-type detecting card - Google Patents
A, B, and O blood-type detecting card Download PDFInfo
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- CN201522495U CN201522495U CN2009202222364U CN200920222236U CN201522495U CN 201522495 U CN201522495 U CN 201522495U CN 2009202222364 U CN2009202222364 U CN 2009202222364U CN 200920222236 U CN200920222236 U CN 200920222236U CN 201522495 U CN201522495 U CN 201522495U
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- antibody
- blood group
- test card
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Abstract
The utility model discloses an A, B, and O blood-type detecting card, belonging to the technical field of blood-type detection. The A, B, and O blood-type detecting card comprises a carrier and an antibody layer fixed on the carrier, wherein the antibody layer is made up of at least two letter-shaped antibody imprints. The detecting card is simple and convenient to operate, can directly read the blood-type result, shortens the detecting time, and can avoid the errors produced by anthropogenic factors; and in the utility model, the antibody is fixed on the carrier, thereby facilitating the conservation and transportation, thoroughly overcoming inconvenience caused by antibody solution in the prior art, and being easily popularized and applied.
Description
Technical field
The utility model relates to a kind of abo blood group test card, belongs to blood group detection technique field.
Background technology
Blood group detects mainly according to the blood group positive definite form, and blood group positive definite form at present is according to the agglutinating reaction of blood group antibody with corresponding red cell antigens always.
Abo blood group is determined as present modal blood group and detects: the red blood cells of type A surface contains A antigen, and blood serum contains B antibody; The Type B erythrocyte surface contains B antigen, contains A antibody in the blood serum; AB type erythrocyte surface contains AB antigen, does not contain AB antibody in the blood serum; 0 type blood surface does not contain AB antigen, contains AB antibody in the blood serum.The agglutinating reaction that this antigen-antibody forms comes sentence read result by the medical professional according to blood grouping reagent.
The blood group positive definite form detects and mainly to comprise for 4 steps: 1, add blood grouping reagent on reaction vessel or flat board, 2, add a small amount of person under inspection's whole blood, 3, with both mixings, 4, sentence read result.Blood group detects and the result judges and must be finished by the medical professional, and (the reagent position is obscured/remembered unclear, sample add wrong position etc.) causes blood group misjudgment as a result to cause serious transfusion reaction even death because multiple human factor.
Testing process need be used antibody-solutions, and the storage temperature of antibody-solutions is generally 4 degrees centigrade, to prevent inefficacy.There is big difficulty in liquid antibody reagent aspect transportation and preservation, especially (for example open-air) usually can can't obtain testing result accurately because antibody lost efficacy when using in the environment of not stablizing refrigerating equipment.
For simplifying the blood group testing process, detection mode easily is provided, people have carried out following trial:
Chinese patent application 200320121646.2 (publication number is 2669196) discloses a kind of detection abo blood group test card.The blood group card is made of the antibody membrane of band groove flat carrier and groove inner drying, and antibody membrane is respectively anti-A, anti-B and panagglutinin.The transparent non-setting adhesive film is used to cover blood group card mark district and the detection zone that detection finishes, and is used to paste archives.Test card, transparent non-setting adhesive film and the drying agent aluminium foil bag of packing into becomes a kit.This test card room temperature preservation, the result is accurate, can effectively control the effect phase, has made things convenient for layman's use, and the result preserves and files conveniently, and single part of mensuration can be carried.Be mainly used in hospital, army, department of the public security organs abo blood group of going out and detect, the individual also can carry out the blood group self check.
The blood group identification card of Chinese patent application 03215617.0 (publication number is 2605574), a kind of easy blood group identification card is disclosed, the integrated circuit board shape, its integrated circuit board is provided with the evaluation pit, pit is provided with identification marking, preferably will identify three of pit designs, and be respectively anti-A and identify that pit, anti-B identify that pit and RH identify pit; Use the papery integrated circuit board, can have to set up defences on the surface of identifying pit at integrated circuit board and ooze rete.The utility model is simple in structure, use easy to operate, the result quick and precisely, cost is low again, is easy to penetration and promotion and uses.
Chinese patent ZL 200620112928.X (publication number is 2901313) discloses a kind of abo blood group reverse type kit, comprise three air-tight bottles, the A type, Type B and O type red blood cell reagent, air-tight bottle is a plastic dropping bottle, by transparent plastic bottle, plastic bottle closure, dropper and sealing gasket are formed, the A type, Type B and O type red blood cell reagent are by the A type, Type B and O type red blood cell and rbc preservative are formed, the A type, Type B and the erythrocytic concentration of O type are 4%, the A type, Type B and O type red blood cell are the red blood cells after filtering by leucocyte filter, and rbc preservative is to use medicine and distilled water formulated according to special ratios.The term of validity of the present utility model was not less than 3 months, the utility model has the advantages that easy to use, the term of validity long, highly sensitive, abo blood group reverse type kit that accuracy is good, can diagnose abo blood group clinically exactly.
Above improvement all can't thoroughly eliminate because the interpretation mistake that the people causes for maloperation.Therefore, need at present a kind ofly can significantly reduce blood group interpretation error rate, simple more, the conspicuous blood type test card of result of operation.
The utility model content
The technical problems to be solved in the utility model is: provide a kind of easy to use, the novel blood type test card that reliable results is obvious.
For achieving the above object, the utility model adopts following design proposal:
A kind of abo blood group test card is made up of carrier and the antibody layer that is fixed on the carrier, and antibody layer is made up of the antibody trace of two letter shapes at least.
A kind of abo blood group test card is made up of carrier and the antibody layer that is fixed on the carrier, and described antibody layer is made up of at least two detecting units, and each detecting unit is made up of the antibody trace of two letter shapes at least.This test card is many person-portions test card, can realize simultaneously a plurality of persons under inspection being carried out blood group determination.
The antibody trace of described single-letter shape is different letter shapes.
The antibody trace of described single-letter shape is alphabetical A shape and letter b shape.
Antibody in the described antibody layer is the golden labeling antibody of polyclonal antibody, monoclonal antibody or colloid gold label, the high specificity of monoclonal antibody and golden labeling antibody wherein, but the sensitivity of intensified response.Polyclonal antibody and monoclonal antibody all can be bought by commercial sources and obtain, and also can adopt known polyclonal antibody or MONOCLONAL ANTIBODIES SPECIFIC FOR method to make.
Described antibody trace is detected by blood group and makes with antibody, and detection antibody wherein is anti-A antibody and anti-B antibody.The letter shapes of antibody trace is alphabetical accordingly with the blood group of this antibody trace generation antigen-antibody reaction.The antibody trace of letter A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.
Described carrier is selected from a kind of in cardboard, glass sheet, plastic plate, tunica fibrosa or the resin plate; Described plastic plate is selected from a kind of in the polymer sheets such as polystyrene, tygon, Polyvinylchloride, polypropylene or polycarbonate; Described tunica fibrosa is selected from a kind of in fibroin protein film, polylactic acid membrane glass fibre membrane, cellulose acetate membrane or the nitrocellulose filter; Described resin plate is selected from a kind of in PVC, ABS, epoxy resin or the phenolics plate.
The local surfaces of described carrier one side surface or a side has radian.
The local surfaces of described carrier one side surface or a side has radian, is convex-surface type or concave structure.
The outer periphery of described convex-surface type structure is fluted.Have in the structure of convex-surface type, antibody layer is positioned at the convex surface place, and the blood with antibody response does not leave the antibody layer surface automatically, can reduce the interference that the result judges.
Described blood type test card is provided with wrappage outward, is selected from plastic sheeting, aluminium foil or the paper one or more.Described plastic sheeting, aluminium foil or paper all are normally used wrappage in drug packaging.Can use the homogenous material packing, for example only be to use plastic film packaging or use In Aluminium Foil Packing, also can use multiple material packing, for example use In Aluminium Foil Packing earlier, again in its outside wrapper.
The utility model blood type test card using method and criterion: add a small amount of person under inspection's whole blood on blood type test card, normally 1-2 drips whole blood, shakes up the back according to the red letter that shows on the blood type test card, just can know the blood group result intuitively.The red alphabetical A that for example shows on the blood type test card represents that person under inspection's blood group is an A type blood, show that red letter b represents that person under inspection's blood group is a Type B blood, show that simultaneously red alphabetical A and letter b represent that person under inspection's blood group is an AB type blood, do not show that any letter judges that its blood group is the O type.
The utility model has the advantages that: the utility model on carrier, needn't re-use the antibody-solutions that refrigerates in the prior art with antibody immobilization, has thoroughly solved a transportation and a refrigeration difficult problem of carrying antibody-solutions, and has greatly simplified detecting operation.The utility model is simple to operate, only need droplets of whole blood is added in reaction place, shake a little, can be according to the direct interpretation blood group result of the letter that manifests behind the erythrocyte aggregation, only need just direct knowledge of result of 2 steps, greatly shortened detection time, the blood group misjudgment that can also avoid human factor to cause, for example contingent blood grouping reagent adds mistake, adds mistakes such as anti-, that blood group result misreads in traditional blood group detecting operation.The utility model is with a wide range of applications as detecting blood group in Blood Center, hospital, accident, battlefield medical treatment, backcountry rescue or family.
The utility model is described in further detail below in conjunction with the drawings and specific embodiments; it is not qualification to utility model; according to prior art well known in the art; embodiment of the present utility model is not limited to this; therefore all this areas of being made according to this paper disclosure be equal to replacement, all belong to protection domain of the present utility model.
Description of drawings
Fig. 1 is first kind of structural representation of the utility model, and carrier is a planar structure.
Fig. 2 is the longitudinal diagram of first kind of structure of the utility model.
Fig. 3 is second kind of structural representation of the utility model, and carrier has concave surface.
Fig. 4 is the longitudinal diagram of second kind of structure of the utility model.
Fig. 5 is the third structural representation of the utility model, and carrier has convex surface and groove.
Fig. 6 is the longitudinal diagram of the third structure of the utility model.
Fig. 7 is the synoptic diagram of person-time blood type test card.
Embodiment
Consult illustrated in figures 1 and 2ly, the abo blood group test card is made up of with antibody layer 2 plane slide carrier 1 and the detection that is fixed on the slide carrier 1, and antibody layer 2 is made up of the antibody trace of two single-letter shapes.
The letter shapes of antibody trace is respectively alphabetical A and letter b, and the antibody trace of alphabetical A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.
Consult Fig. 3 and shown in Figure 4, the abo blood group test card is made up of with antibody layer 2 cardboard carrier 1 and the detection that is fixed on the cardboard carrier 1, and antibody layer 2 is made up of the antibody trace of two single-letter shapes.
The letter shapes of antibody trace is respectively alphabetical A and letter b, and the antibody trace of alphabetical A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.
The surveyed area of cardboard carrier 1 has concave structure 3, and the antibody trace is positioned at concave structure 3 places.
Consult Fig. 5 and shown in Figure 6, the abo blood group test card is made up of with antibody layer 2 cardboard carrier 1 and the detection that is fixed on the cardboard carrier 1, and antibody layer 2 is made up of the antibody trace of two single-letter shapes.
The letter shapes of antibody trace is respectively alphabetical A and letter b, and the antibody trace of alphabetical A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.
The surveyed area of cardboard carrier 1 has convex configuration 3, and the antibody trace is positioned at convex configuration 3 places, and being peripherally installed with of convex configuration 3 is fluted 4, can collect unreacted blood.
Consult shown in Figure 7ly, the abo blood group test card is made up of cardboard carrier 1 and the antibody layer 2 that is fixed on the cardboard carrier 1, and antibody layer 2 is made up of several detecting units, and each detecting unit is made up of the antibody trace of two single-letter shapes.
The letter shapes of the antibody trace in each detecting unit is respectively alphabetical A and letter b, and the antibody trace of alphabetical A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.
The surveyed area of cardboard carrier 1 has convex configuration 3, and antibody layer is positioned at convex configuration 3 places, and being peripherally installed with of convex configuration 3 is fluted 4, is used to collect unreacted blood.
This abo blood group test card can realize that simultaneously many people being carried out blood group detects, and the edge blank space can be done the usefulness of detection record.
Embodiment 5: adopt cardboard to produce the utility model blood type test card as carrier
Adopt the production method of medical glass slide glass as the blood type test card of carrier
A, select for use medical glass slide glass (slide) as carrier, its washing methods is to soak diel with 1% HCl solution, scrubs with commercially available washing agent again, washes repeatedly with clear water then, uses distilled water flushing at last 1～2 time.After filter is done, in drying box, under 90 ℃ of temperature, carry out dry 2h again, be cooled to room temperature then.
B, the 3-aminopropyl triethoxysilane of selling with Shandong Province Qufu City China flourish new chemical materials company limited on the cleaning carrier are handled, and take out slide with this reagent after with the slide submergence, and drying is standby;
C, spray the antibody trace of alphabetical A and B shape with corresponding antibodies on treated carrier, the antibody trace of alphabetical A shape adopts the anti-A antibody spraying, and the antibody trace of letter b shape adopts anti-B antibody spraying, spend the night (placing 0.5-24 hour); (antibody is available from Shanghai blood biomedical limited liability company, and commodity are by name: anti-A, anti-B blood grouping reagent)
D, remove not and carrier-bound unnecessary antibody, dry with phosphate buffer.
E, the blood type test card that obtains pack with the aluminium pool, and many person-portions or single part all can.
Use the embodiment 1 described single part of abo blood group test card of the method production of embodiment 5 to carry out 100 person-times of detections, the result shows, the testing result that non-medical professional uses this blood type test card to obtain is identical with the testing result that present medical professional adopts traditional blood type testing methods to obtain, non-medical professional uses this blood type test card detection speed fast again, produces cost in batches and can be lower than existing traditional blood type testing methods.Therefore, be very beneficial for wide popularization and application.
Claims (10)
1. abo blood group test card is characterized in that: be made up of carrier and the antibody layer that is fixed on the carrier, antibody layer is made up of the antibody trace of two letter shapes at least.
2. a kind of abo blood group test card according to claim 1, it is characterized in that: described antibody layer is made up of at least two detecting units, and each detecting unit is made up of the antibody trace of two letter shapes at least.
3. a kind of abo blood group test card according to claim 1 and 2 is characterized in that: the antibody trace of described letter shapes is different letter shapes.
4. a kind of abo blood group test card according to claim 3 is characterized in that: the antibody trace of described letter shapes is alphabetical A shape and letter b shape.
5. a kind of abo blood group test card according to claim 3 is characterized in that: described antibody trace is detected by blood group and makes with antibody, and the antibody trace of alphabetical A shape is an anti-A antibody, and the antibody trace of letter b shape is an anti-B antibody.
6. a kind of abo blood group test card according to claim 1 and 2 is characterized in that: described carrier is selected from a kind of in cardboard, glass sheet, plastic plate, tunica fibrosa or the resin plate; Described plastic plate is selected from a kind of in polystyrene, tygon, Polyvinylchloride, polypropylene or the polycarbonate plate; Described tunica fibrosa is selected from a kind of in fibroin protein film, polylactic acid membrane glass fibre membrane, cellulose acetate membrane or the nitrocellulose filter; Described resin plate is selected from a kind of in PVC, ABS, epoxy resin or the phenolics plate.
7. a kind of abo blood group test card according to claim 1 and 2 is characterized in that: the local surfaces of described carrier one side surface or a side has radian.
8. a kind of abo blood group test card according to claim 7 is characterized in that: the local surfaces of described carrier one side surface or a side has radian, is convex-surface type or concave structure.
9. a kind of abo blood group test card according to claim 7, it is characterized in that: the outer periphery of described convex-surface type structure is fluted.
10. a kind of abo blood group test card according to claim 1 and 2, it is characterized in that: described blood type test card is provided with wrappage outward, is selected from plastic sheeting, aluminium foil or the paper one or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202222364U CN201522495U (en) | 2009-08-28 | 2009-08-28 | A, B, and O blood-type detecting card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202222364U CN201522495U (en) | 2009-08-28 | 2009-08-28 | A, B, and O blood-type detecting card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201522495U true CN201522495U (en) | 2010-07-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009202222364U Expired - Fee Related CN201522495U (en) | 2009-08-28 | 2009-08-28 | A, B, and O blood-type detecting card |
Country Status (1)
| Country | Link |
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| CN (1) | CN201522495U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103884849A (en) * | 2014-04-06 | 2014-06-25 | 邵超鹏 | Display manner of determination results of blood type and blood type antibody and manufacturing method |
| CN104569444A (en) * | 2014-12-22 | 2015-04-29 | 合肥天一生物技术研究所 | Forward and reverse ABO typing and RhD blood type detecting card |
-
2009
- 2009-08-28 CN CN2009202222364U patent/CN201522495U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103884849A (en) * | 2014-04-06 | 2014-06-25 | 邵超鹏 | Display manner of determination results of blood type and blood type antibody and manufacturing method |
| CN104569444A (en) * | 2014-12-22 | 2015-04-29 | 合肥天一生物技术研究所 | Forward and reverse ABO typing and RhD blood type detecting card |
| CN104569444B (en) * | 2014-12-22 | 2016-03-30 | 合肥天一生物技术研究所 | A kind of positive reverse type of ABO and RhD blood type test card |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100707 Termination date: 20140828 |
|
| EXPY | Termination of patent right or utility model |