CN105092567B - Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol - Google Patents
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol Download PDFInfo
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- CN105092567B CN105092567B CN201510416292.1A CN201510416292A CN105092567B CN 105092567 B CN105092567 B CN 105092567B CN 201510416292 A CN201510416292 A CN 201510416292A CN 105092567 B CN105092567 B CN 105092567B
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Abstract
The present invention relates to a kind of three link detection reagent cards of clenobuterol hydrochloride-Ractopamine-salbutamol.The three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol includes reagent strip and shell, the reagent strip is contained in the shell, the reagent strip includes protective film, bottom plate and set on the bottom plate and it is sequentially connected the sample absorbent of setting, colloidal gold pad, nitrocellulose filter and water absorption pad, the protective film is set to a side surface of the nitrocellulose filter, the colloidal gold pad is coated with anti-Clenizole Hydrochloride antibody, anti- salbutamol antibody and anti-Anti-ractopamine antibody, the nitrocellulose filter contains the three detections line being sequentially arranged at intervals and a nature controlling line.The three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention can effectively avoid air environment factor to the chromogenic impact of detection line on reagent card, have the advantages that detection effect is good and can apply to quantitative detection.
Description
Technical field
The present invention relates to beta-receptor agonist detection technique fields, and in particular to a kind of clenobuterol hydrochloride-Lake DOPA
Three link detection reagent card of amine-salbutamol.
Background technique
Beta-receptor agonist is a kind of artificial synthesized adrenoceptor agonists, including clenobuterol hydrochloride, Sha Ding
Amine alcohol and Ractopamine, also known as be clenbuterol hydrochloride, it is usually used in preventing and treating the pulmonary diseases such as asthma and pulmonary emphysema.When its dosage reaches
When to 5-10 times of therapeutic dose, has the function of adjusting nutriment flow direction and redistribute, the synthesis of body muscle can be made to increase,
Fat deposition is reduced.If large dosage of use can significantly improve feed conversion rate and lean meat percentage in husbandry sector, but also can simultaneously
The residual of clenbuterol hydrochloride in animal food is caused, toxicological effect can be generated to internal organs such as liver kidneys after eating, palpitaition, head occur
Bitterly, the symptoms such as dizzy and muscle chatter even will cause death so as to cause the generation of the mankind's poisoning when serious.Therefore,
Countries in the world government generally all forbids the feed addictive for using clenobuterol hydrochloride as farming animals.The Ministry of Agriculture of China is 2002
Also clearly forbid adding the excitement of the beta-receptors such as clenobuterol hydrochloride in feed in No. 235 bulletin of publication on December 24, in
Agent, and provide that the beta-receptors such as clenobuterol hydrochloride agonist residual quantity must not be in animal food and detect.Therefore, effectively
Detection method is the key that one of beta-receptors agonist abuse such as control clenobuterol hydrochloride, it is necessary to which research is highly sensitive quickly
The method for detecting the beta-receptors agonists such as clenobuterol hydrochloride.
Currently, the method for the beta-receptors agonist such as common detection clenobuterol hydrochloride mainly has gas chromatography-mass spectrography, height
Effect liquid phase chromatogram method, enzyme-linked immunization and colloidal gold immunity chromatography etc..Wherein, colloidal gold immunity chromatography is based on immunochromatography
Technical know-how, by preparing immune-gold labeled reagent card, to beta-receptors such as clenobuterol hydrochlorides in animals urine or tissue samples
Agonist is detected, and whether there is the beta-receptors agonists such as clenobuterol hydrochloride in animality meat product with this to qualitatively judge
Residual.But existing technology is usually individually to beta-receptor agonist three kinds of ingredient clenobuterol hydrochlorides, salbutamols respectively
With in Ractopamine one of or combine detected two-by-two, and it is rare to the three the relevant technologies and production that are detected simultaneously
Product.
In addition, existing utilize the beta-receptors agonist detection reagent card such as clenobuterol hydrochloride of colloidal gold immunity chromatography
Have the shortcomings that detection sensitivity is not high and may not apply to quantitative testing device.The reason is that due to reagent card colour developing portion
Divide and be directly exposed in air, thus easily cause influence of the environmental factor to reagent card, to cause detection line on reagent card
Color it is unstable, cause profound color with it is extremely light when by naked eyes determine the case where being likely to occur false positive and false negative hair
It is raw, it causes to judge by accident, to influence the food safety of the people；In addition, when needing to the excitement of the beta-receptors such as clenobuterol hydrochloride
When agent residual carries out quantitative detection, since the beta-receptors agonist gold-immunochromatographyreagent reagent for assay card such as current clenobuterol hydrochloride exists
Drawbacks described above, cause environmental factor to reagent card detection line color influence so that reagent card detection line color it is unstable
And distortion can be further amplified when reading data in distortion situation aggravation, the photoelectric sensor based on this reagent card detection line color
Effect, be finally reflected quantitative judgement numerically, will have serious inaccuracy, thus cause to be commonly used
The beta-receptors agonist detection reagent card such as clenobuterol hydrochloride can not be applied to quantitative detection or qualitative detection is ineffective.
Summary of the invention
Exist to solve the beta-receptors agonists such as above-mentioned clenobuterol hydrochloride detection reagent card vulnerable to air environment factor
It influences and causes the color of detection line on reagent card unstable, it is bad so as to cause detection effect and quantitative detection can not be applied to
The technical issues of, the present invention provides a kind of air environment factor that can effectively avoid to the chromogenic impact of detection line on reagent card, inspection
Survey effect is good and can apply to the three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol of quantitative detection.
The present invention provides a kind of three link detection reagent cards of clenobuterol hydrochloride-Ractopamine-salbutamol, including examination
The shell of agent item and tool accommodating space, the reagent strip are contained in the shell, and the reagent strip includes protective film, bottom plate and sets
In the bottom plate and it is sequentially connected sample absorbent, colloidal gold pad, nitrocellulose filter and the water absorption pad of setting, the protective film
Set on a side surface of the nitrocellulose filter, the colloidal gold pad is coated with anti-Clenizole Hydrochloride antibody, desertification butylamine
Alcohol antibody and anti-Anti-ractopamine antibody, the nitrocellulose filter contain the three detections line being sequentially arranged at intervals and a matter
Control line, the three detections line is arranged close to the colloidal gold pad, be respectively coated with clenobuterol hydrochloride protein conjugate,
The detection line of salbutamol protein conjugate and Ractopamine protein conjugate, the nature controlling line are coated with sheep or rabbit-anti
Mouse antibody and the close water absorption pad.
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention it is a kind of compared with
In good embodiment, the bottom plate is support plate made of PVC material.
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention it is a kind of compared with
In good embodiment, the sample absorbent includes the groove set on one end, and described colloidal gold pad one end is located in the sample
In the groove of absorption pad.
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention it is a kind of compared with
In good embodiment, the colloidal gold pad includes the slot set on one end, and described nitrocellulose filter one end is located in the glue
In the slot of body gold pad.
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention it is a kind of compared with
In good embodiment, the water absorption pad includes the socket set on one end, and the nitrocellulose filter other end is located in the suction
In the socket of water cushion.
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention it is a kind of compared with
In good embodiment, the shell is bar shaped shell open at one end and inner hollow, and the open end is for the reagent strip
It is inserted and removed from；The shell includes the drop sample window and display window through the setting of one side surface, described in the drop sample window face
Sample absorbent is arranged, the setting of nitrocellulose filter described in the display window face.
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention it is a kind of compared with
In good embodiment, the shell further includes the cutting ferrule set on its outer surface, to contain the storage of the card of sample information.
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention it is a kind of compared with
In good embodiment, the card comprising sample information is bar code or two dimensional code card.
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention it is a kind of compared with
In good embodiment, the protective film is the anti-oxidation polyethylene protective film of air insulated.
Compared to the prior art, clenobuterol hydrochloride provided by the invention-three joint inspection of Ractopamine-salbutamol test
Agent card has the advantages that
One, protective film is added by the side surface in nitrocellulose filter, so that nitrocellulose filter can effectively avoid
Air environment factor is to the chromogenic impact of detection line on nitrocellulose filter, so that colour developing is more stable, better effect is convenient
It is accurate to qualitatively judge testing result；And make three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol can also
Applied to quantitative detection, it is more true and reliable to quantitatively judge data；
Two, adding by protective film, so that clenobuterol hydrochloride-three link detection reagent of Ractopamine-salbutamol
Card avoids the influence of environmental factor, and storage time is more long, convenient transportation and it is non-at-scene provide in real time as a result, can will test after
Reagent card, which is concentrated, to be saved, and analysis is uniformly processed, reduces workload, reduces human time's cost；
Three, by the way that sample absorbent is designed as one end with groove, colloidal gold pad one end is located in sample absorbent
In groove；Colloidal gold pad is designed as one end with slot, and nitrocellulose filter one end is located in the slot of colloidal gold pad；Water suction
Pad is designed as one end with socket, and the nitrocellulose filter other end is located in the socket of water absorption pad；So that sample to be tested instills
After sample absorbent, sandwich design effectively promotes chromatographic effect so that antigen, antibody and water chromatography, permeate and migrate more
Advantage significantly improves the accuracy of testing result；
Four, by designing cutting ferrule in housing outer surface, for storing bar coded cards or two dimensional code containing sample information
Card, so that testing result and sample information accurately correspond to, reduces and intersects conveniently in pattern detection to the reading of sample information
The risk of judgement；
Five, by using bottom plate made of PVC material and using air insulated protection against oxidation made of polythene material
Film, reduces the cost of three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol, and improves durability
And storage time；
Six, it is successfully realized three kinds of clenobuterol hydrochloride, Ractopamine, salbutamol beta-receptor agonists for the first time simultaneously
Detection, and detection effect is good and can carry out quantitative analysis using quantitative detection instrument.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing, in which:
Fig. 1 is the appearance of three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention
Figure；
Fig. 2 is the reagent strip of three link detection reagent cards of clenobuterol hydrochloride-Ractopamine-salbutamol shown in Fig. 1
Schematic cross-section；
Fig. 3 is the reagent strip of three link detection reagent cards of clenobuterol hydrochloride-Ractopamine-salbutamol shown in Fig. 2
Top view.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that the described embodiments are merely a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts all other
Embodiment shall fall within the protection scope of the present invention.
Also referring to Fig. 1, Fig. 2 and Fig. 3, wherein Fig. 1 is clenobuterol hydrochloride-Ractopamine-provided by the invention
The outside drawing of three link detection reagent card of salbutamol；Fig. 2 is clenobuterol hydrochloride-Ractopamine-salbutamol three shown in Fig. 1
The schematic cross-section of the reagent strip of link detection reagent card；Fig. 3 is clenobuterol hydrochloride-Ractopamine-salbutamol shown in Fig. 2
The top view of the reagent strip of three link detection reagent cards.The clenobuterol hydrochloride-three joint inspection of Ractopamine-salbutamol test
Agent card 1 includes reagent strip 11 and the shell 13 for having accommodating space, and the reagent strip 11 is contained in the shell 13.
The reagent strip 11 includes bottom plate 111 and sample absorbent 112, colloidal gold pad 113, nitrocellulose filter 114, inhales
Water cushion 115 and protective film 116.The sample absorbent 112, colloidal gold pad 113, nitrocellulose filter 114 and water absorption pad 115 according to
Secondary connection is simultaneously set to the bottom plate 111, and the protective film 116 is set to a side surface of the nitrocellulose filter 114.
The bottom plate 111 is support plate made of PVC material, has preferable impermeable ability, buffer action and reflective
Effect.
The sample absorbent 112 is glass fibre membrane, has preferable animals urine sample, feed sample and tissue sample
This absorbability, the sample-adding for sample to be tested.The sample absorbent 112 includes the groove 1121 set on one end, institute
It states 113 one end of colloidal gold pad to be located in the groove 1121 of the sample absorbent, by the design of the groove 1121, increase
The contact area of the sample absorbent 112 and the colloidal gold pad 113, is conducive to be loaded sample and passes through the sample absorbance
The chromatography of pad 112 penetrates into the colloidal gold pad 113.
The colloidal gold pad 113 is the glass fibre membrane containing colloid gold particle, and colloid gold particle pan coating has can
To identify clenobuterol hydrochloride, salbutamol and the antibody of Ractopamine.The colloidal gold pad 113 includes being set to one end
Slot 1131, described 114 one end of nitrocellulose filter are located in the slot 1131 of the colloidal gold pad, pass through the slot
1131 design increases the contact area of the colloidal gold pad 113 and the nitrocellulose filter 114, it is logical to be conducive to sample
It crosses the chromatography of colloidal gold pad 113 and penetrates into the nitrocellulose filter 114.
Three detections line 1141,1142,1143 and one of the nitrocellulose filter 114 containing successively spaced and parallel arrangement
Nature controlling line 1145, wherein detection line 1141 is to be coated with the detection line of clenobuterol hydrochloride protein conjugate, detection line
1142 be the detection line for being coated with salbutamol protein conjugate, and detection line 1143 is to be coated with Ractopamine protein idol
Join the detection line of object, three is arranged close to 113 side of colloidal gold pad, and the nature controlling line 1145 is coated with sheep or rabbit-anti mouse is anti-
Body is simultaneously arranged close to 115 side of water absorption pad, by the nitrocellulose filter 114 whether to qualitatively judge detection sample
Remain folic acid Clenbuterol, specifically:
If 1) three detections line 1141,1142,1143 and nature controlling line 1145 show aubergine simultaneously, indicate that result is yin
Property, illustrate to be detected clenobuterol hydrochloride, salbutamol and Ractopamine content in sample and is not above permissible value (3ng/
ml)；
2) if display color, nature controlling line 1145 do not show that aubergine, testing result are to detection line 1141,1142,1143
The positive shows to be detected clenobuterol hydrochloride, salbutamol and Ractopamine content in sample while being more than permissible value (3ng/
ml)；
If 3) display color, detection line 1143 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1141,1142,
Testing result is the positive, and showing to be detected clenobuterol hydrochloride and salbutamol content in sample is more than permissible value (3ng/ml),
And Ractopamine content is not above permissible value (3ng/ml)；
If 4) display color, detection line 1142 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1141,1143,
Testing result is the positive, and showing to be detected clenobuterol hydrochloride and Ractopamine content in sample is more than permissible value (3ng/
Ml), and salbutamol content is not above permissible value (3ng/ml)；
If 5) display color, detection line 1141 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1142,1143,
Testing result is the positive, and showing to be detected salbutamol and Ractopamine content in sample is more than permissible value (3ng/ml), and
Clenobuterol hydrochloride content is not above permissible value (3ng/ml)；
If 6) display color, detection line 1142,1143 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1141,
Testing result is the positive, and showing to be detected clenobuterol hydrochloride content in sample is more than permissible value (3ng/ml), and salbutamol
Permissible value (3ng/ml) is not above with Ractopamine content；
If 7) display color, detection line 1141,1143 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1142,
Testing result is the positive, and showing to be detected salbutamol content in sample is more than permissible value (3ng/ml), and clenobuterol hydrochloride
Permissible value (3ng/ml) is not above with Ractopamine content；
If 8) display color, detection line 1141,1142 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1143,
Testing result is the positive, and showing to be detected Ractopamine content in sample is more than permissible value (3ng/ml), and hydrochloric acid Ke Lunte
Sieve and salbutamol content are not above permissible value (3ng/ml)；
In addition, being provided the phenomenon that the aubergine that detection line 1141,1142,1143 occurs can show shade
Observing time in (within 1 month), no matter detection line shade is determined as negative findings；
9) if detection line 1141,1142,1143 and nature controlling line 1145 do not develop the color, test crash or test paper are shown
Failure.
The water absorption pad 115 includes the socket 1151 set on one end, and 114 other end of nitrocellulose filter is located in
In the socket 1151 of the water absorption pad, by the design of the socket 1151, it is fine with the nitric acid to increase the water absorption pad 115
The contact area for tieing up plain film 114 is conducive to moisture chromatography in sample and penetrates into the water absorption pad 115.
The protective film 116 is the anti-oxidation polyethylene protective film of air insulated, is set to the nitrocellulose filter 114 and institute
State the opposite flank of 111 contact surface of bottom plate.It acts as so that the nitrocellulose filter 114 can effectively avoid air ring
Border factor is to the chromogenic impact of detection line 1141 on the nitrocellulose filter, so that colour developing is more stable, more longlasting, effect
More preferably, convenient accurate qualitative judgement testing result, and the validity period of the reagent strip 11 is significantly extended.
The shell 13 is bar shaped shell open at one end and inner hollow, and the open end is for the reagent strip 11
It is inserted and removed from.The shell 13 includes the drop sample window 131 and display window 133 through the setting of one side surface, the drop sample window
Sample absorbent 112 described in 131 faces is arranged, and nitrocellulose filter 114 described in 133 face of display window is arranged.Pass through institute
It states drop sample window 131 and carries out sample-adding operation, testing result is observed by the display window 133.
The shell 13 further includes the cutting ferrule 135 set on its outer surface, to contain the storage of the card of sample information, institute
Stating the card comprising sample information is bar code or two dimensional code card, the typing and export of convenience sample information.
The preparation method of the three link detection reagent card 1 of clenobuterol hydrochloride-Ractopamine-salbutamol:
1) laboratory apparatus and reagent:
PVC board, glass fibre membrane, nitrocellulose filter, cutting machine, desk centrifuge, vacuum freeze drier etc.；Hydrochloric acid
Clenbuterol standard items, sheep or rabbit anti-mouse antibody (Sigma company), anti-salbutamol antibody, resist salt resistance acid Ke Lunte wheel antibody
Anti-ractopamine antibody and clenobuterol hydrochloride-bovine serum albumin conjugate, salbutamol-bovine serum albumin conjugate, Lake
Dopamine-bovine serum albumin conjugate by experiment synthesize, macrogol, polyethylene glycol, glycine, bovine serum albumin, 1%
Gold chloride, 1% sodium citrate, Tris-HCL buffer (PH=7.5) and distilled water etc..
2) preparation of colloidal gold solution:
It takes 500mL distilled water in reagent bottle, the gold chloride of 2500 μ L is first added dropwise, is heated to boiling, then be added dropwise into 2500 μ
L sodium citrate, observation solution colour variation are cold after small fire heats 5-10 minutes when it gradually becomes red from aubergine
But, then to be stored in 4 DEG C of refrigerators stand-by.
3) preparation of colloidal gold pad:
5mL Tris-HCL buffer and 20 μ L salt resistance acid Ke Lunte is taken to take turns antibody, anti-salbutamol antibody and anti-Lay respectively
Gram dopamine antibody is mixed and is shaken up, and is added 50mL colloidal gold solution, is placed 5-10 minutes in ice bath later, and will mixing
Liquid PH is adjusted to 8.5 or more；After standing 30-40 minutes, 600 μ L bovine serum albumins are continuously added, stand 5-10 minutes later, storage
In centrifuge tube, and with 12000r/min centrifugal treating, red precipitate is generated, then washed liquid washs 2-3 times, by red precipitate
It drops evenly and the colloidal gold pad is made in glass fibre membrane, freezen protective is stand-by；
By clenobuterol hydrochloride-bovine serum albumin conjugate, salbutamol-bovine serum albumin conjugate and Lake DOPA
Parallel interval is sprayed on nitrocellulose filter respectively for amine-bovine serum albumin conjugate and sheep/rabbit anti-mouse igg antibody, using true
Vacuum freecing-dry machine carries out low-temperature vacuum drying, saves stand-by.
4) encapsulation of reagent strip:
Stand-by nitrocellulose filter is saved before installing on PVC bottom plate, before nitrocellulose filter installation
The case where it is first blockaded, is realized using 25g/L bovine serum albumin and 1.5g glycine, non-specific binding is eliminated；
Then one layer of anti-oxidation polyethylene of air insulated is assembled in the opposite flank of nitrocellulose filter and the PVC base plate contact surface
Protective film；And then successively assemble sample absorbent, colloidal gold pad and water absorption pad；Add finally, carrying out cutting using cutting machine
The reagent strip of 6cm × 1cm is made in work, dries spare.
5) preparation of reagent card:
The bar shaped shell open at one end and inner hollow of a collection of producer's customization, hollow space size and the reagent are provided
Size is adapted, and the reagent strip is inserted into and is engaged by the open end of shell；The shell includes running through one side table
The drop sample window and display window of face setting, sample absorbent described in the drop sample window face are arranged, nitre described in the display window face
The setting of acid cellulose film, finally carries out sealing packaging, so far clenobuterol hydrochloride-Ractopamine-the salbutamol three
Detection reagent blocking is standby to be finished, and low temperature seal environment is stored in, spare.
The application method of the three link detection reagent card 1 of clenobuterol hydrochloride-Ractopamine-salbutamol:
1) preparation of sample:
Urine specimen: random sampling a certain amount of livestock urine 3000r/min centrifugally operated 5 minutes, takes 100 μ L of supernatant
Detection, detection liquid extension rate are 1；
Feed sample: the 0.1M HCL solution of 20ml, concussion behaviour is added in the feed sample of random sampling 2g homogeneity processing
Make 5 minutes, 3000r/mmin centrifugally operated 10 minutes, takes supernatant 2ml that the 1M NaOH solution of 40 μ L, then 3000r/ is added
Mmin centrifugally operated 10 minutes, 100 μ L supernatants is taken to be detected, detection liquid extension rate is 10；
Tissue samples: random sampling 5g crushes tissue samples, and the trichloroacetic acid of 12ml 3% is added, and concussion is mixed by inversion,
3000r/mmin centrifugally operated 10 minutes；It takes supernatant 6ml to add 6ml isopropanol-ethyl acetate mixtures, vibrates 10 minutes, it is quiet
It sets 5 minutes；3000r/mmin centrifugally operated 10 minutes again take 100 μ L supernatants to be detected, and detection liquid extension rate is 5；
2) sample-adding detection:
The three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol prepared is taken out, in room temperature ring
Lower 25 DEG C of -35 DEG C of progress pattern detections in border；
Respectively by clenobuterol hydrochloride, salbutamol and Ractopamine titer 0ng/ml, 1ng/ml, 3ng/ml,
The drop sample window in reagent card is added dropwise in 5ng/ml, 7ng/ml, 9ng/ml respectively, carries out color observation as a control group；It will be to test sample
This drips sample in the drop sample window of reagent card respectively, and 3 drop sample to be tested liquid are successively added dropwise in each drop sample window；
3) result judgement:
Color observation is carried out by the display window of reagent card, initial characterization is carried out to the color of sample to be tested detection card and is sentenced
It is disconnected, to determine in sample to be tested whether contain clenobuterol hydrochloride, salbutamol and Rct opamine residue, specifically:
If a) three detections line 1141,1142,1143 and nature controlling line 1145 show aubergine simultaneously, indicate that result is yin
Property, illustrate to be detected clenobuterol hydrochloride, salbutamol and Ractopamine content in sample and is not above permissible value (3ng/
ml)；
If b) display color, nature controlling line 1145 do not show that aubergine, testing result are to detection line 1141,1142,1143
The positive shows to be detected clenobuterol hydrochloride, salbutamol and Ractopamine content in sample while being more than permissible value (3ng/
ml)；
If c) display color, detection line 1143 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1141,1142,
Testing result is the positive, and showing to be detected clenobuterol hydrochloride and salbutamol content in sample is more than permissible value (3ng/ml),
And Ractopamine content is not above permissible value (3ng/ml)；
If d) display color, detection line 1142 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1141,1143,
Testing result is the positive, and showing to be detected clenobuterol hydrochloride and Ractopamine content in sample is more than permissible value (3ng/
Ml), and salbutamol content is not above permissible value (3ng/ml)；
If e) display color, detection line 1141 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1142,1143,
Testing result is the positive, and showing to be detected salbutamol and Ractopamine content in sample is more than permissible value (3ng/ml), and
Clenobuterol hydrochloride content is not above permissible value (3ng/ml)；
If f) display color, detection line 1142,1143 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1141,
Testing result is the positive, and showing to be detected clenobuterol hydrochloride content in sample is more than permissible value (3ng/ml), and salbutamol
Permissible value (3ng/ml) is not above with Ractopamine content；
If g) display color, detection line 1141,1143 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1142,
Testing result is the positive, and showing to be detected salbutamol content in sample is more than permissible value (3ng/ml), and clenobuterol hydrochloride
Permissible value (3ng/ml) is not above with Ractopamine content；
If h) display color, detection line 1141,1142 and nature controlling line 1145 do not show aubergine simultaneously to detection line 1143,
Testing result is the positive, and showing to be detected Ractopamine content in sample is more than permissible value (3ng/ml), and hydrochloric acid Ke Lunte
Sieve and salbutamol content are not above permissible value (3ng/ml)；
In addition, being provided the phenomenon that the aubergine that detection line 1141,1142,1143 occurs can show shade
Observing time in (within 1 month), no matter detection line shade is determined as negative findings；
If i) detection line 1141,1142,1143 and nature controlling line 1145 do not develop the color, test crash or test paper are shown
Failure.
In addition, can be also compared by the color with control group, further substantially rational judgment is carried out, is understood to be measured
The estimation numerical value of sample clenobuterol hydrochloride, salbutamol and Rct opamine residue.
4) test analysis:
Sample to be tested, salt are detected using the three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol
The rate of recovery of Clenbuterol, salbutamol and Ractopamine is 89.0%-95.5%.Therefore, the clenobuterol hydrochloride-
Three link detection reagent card detection accuracy of Ractopamine-salbutamol is higher, and the extensive detection that can satisfy actual sample is wanted
It asks.
In addition, further test discovery, the clenobuterol hydrochloride-three link detection reagent of Ractopamine-salbutamol
Color after card sample-adding be able to maintain it is constant for a long time, as long as longest storage life can reach 1 month, Color Color without significant change,
Prompt the three link detection reagents card can store more long, and avoid previous reagent card using when need detection process and analysis
The drawbacks of process synchronous progress simultaneously.
Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol provided by the invention has with following
Beneficial effect:
One, protective film 116 is added by the side surface in nitrocellulose filter 114, so that nitrocellulose filter 114 can
Effectively avoid air environment factor to the chromogenic impact of detection line on nitrocellulose filter 114, so that colour developing is more stable, effect
Fruit is more preferably, convenient accurately to qualitatively judge testing result；And detection reagent card is made also to can apply to quantitative detection, quantitatively judge number
According to more true and reliable；
Two, adding by protective film 116, so that detection reagent card avoids the influence of environmental factor, storage time is more long,
Convenient transportation and it is non-at-scene provided in real time as a result, reagent card after can will test is concentrated saves, analysis, reduction work is uniformly processed
It measures, reduces human time's cost；
Three, by the way that sample absorbent 112 is designed as one end with groove 1121,113 one end of colloidal gold pad is located in sample
In the groove 1121 of this absorption pad 112；Colloidal gold pad 113 is designed as one end with slot 1131,114 one end of nitrocellulose filter
It is located in the slot 1131 of colloidal gold pad 113；Water absorption pad 115 is designed as one end with socket 1151, nitrocellulose filter 114
The other end is located in the socket 1151 of water absorption pad 115；So that there is sandwich design after sample to be tested instills sample absorbent 112
Effect promoted chromatographic effect so that antigen, antibody and water chromatography, permeate and migrate more advantage, significantly improve testing result
Accuracy；
Four, by designing cutting ferrule 135 in 13 outer surface of shell, for storing the bar coded cards containing sample information or two
Code card is tieed up, conveniently in pattern detection to the reading of sample information, so that testing result and sample information accurately correspond to, is reduced
Intersect the risk determined；
Five, by using bottom plate made of PVC material and using air insulated protection against oxidation made of polythene material
Film 116, reduces the cost of detection reagent card, and improves durability and storage time；
Six, it is successfully realized three kinds of clenobuterol hydrochloride, Ractopamine, salbutamol beta-receptor agonists for the first time simultaneously
Detection, and detection effect is good and can carry out quantitative analysis using quantitative detection instrument.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright specification and accompanying drawing content is applied directly or indirectly in other relevant skills
Art field, is included within the scope of the present invention.
Claims (6)
1. a kind of three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol, including reagent strip and tool receiving sky
Between shell, the reagent strip is contained in the shell, and the reagent strip includes bottom plate and set on the bottom plate and is sequentially connected
Sample absorbent, colloidal gold pad, nitrocellulose filter and the water absorption pad of setting, which is characterized in that the reagent strip further includes protecting
Cuticula, the protective film are set to a side surface of the nitrocellulose filter, and the colloidal gold pad is coated with salt resistance acid Ke Lunte
Luo Kangti, anti-salbutamol antibody and anti-Anti-ractopamine antibody, the nitrocellulose filter contain three be sequentially arranged at intervals
Detection line and a nature controlling line, the three detections line are arranged close to the colloidal gold pad, are respectively coated with hydrochloric acid gram human relations
The detection line of special sieve protein conjugate, salbutamol protein conjugate and Ractopamine protein conjugate, the matter
Control line is coated with sheep or rabbit anti-mouse antibody and close to the water absorption pad, and the sample absorbent includes the groove set on one end,
Described colloidal gold pad one end is located in the groove of the sample absorbent, and the colloidal gold pad includes inserting set on one end
Slot, described nitrocellulose filter one end are located in the slot of the colloidal gold pad, and the water absorption pad includes being set to one end
Socket, the nitrocellulose filter other end are located in the socket of the water absorption pad.
2. three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol according to claim 1, special
Sign is that the bottom plate is support plate made of PVC material.
3. three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol according to claim 1, special
Sign is that the shell is bar shaped shell open at one end and inner hollow, and the open end is used for the insertion of the reagent strip
And taking-up；The shell includes the drop sample window and display window through the setting of one side surface, sample described in the drop sample window face
Absorption pad is arranged, the setting of nitrocellulose filter described in the display window face.
4. three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol according to claim 3, special
Sign is that the shell further includes the cutting ferrule set on its outer surface, to contain the storage of the card of sample information.
5. three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol according to claim 4, special
Sign is that the card comprising sample information is bar code or two dimensional code card.
6. three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol according to claim 1, special
Sign is that the protective film is the anti-oxidation polyethylene protective film of air insulated.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510416292.1A CN105092567B (en) | 2015-07-15 | 2015-07-15 | Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol |
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| CN201510416292.1A CN105092567B (en) | 2015-07-15 | 2015-07-15 | Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol |
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| CN107132356B (en) * | 2017-04-27 | 2019-02-01 | 北京航空航天大学 | A kind of colloidal gold test paper card and preparation method thereof detecting bladder cancer |
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