CN105044366A - Novel reagent testing card of clenobuterol hydrochloride - Google Patents
Novel reagent testing card of clenobuterol hydrochloride Download PDFInfo
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- CN105044366A CN105044366A CN201510416606.8A CN201510416606A CN105044366A CN 105044366 A CN105044366 A CN 105044366A CN 201510416606 A CN201510416606 A CN 201510416606A CN 105044366 A CN105044366 A CN 105044366A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2430/00—Assays, e.g. immunoassays or enzyme assays, involving synthetic organic compounds as analytes
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Abstract
The invention relates to a novel reagent testing card of clenobuterol hydrochloride. The novel reagent testing card of clenobuterol hydrochloride comprises a reagent strip and a housing with a containing space, wherein the reagent strip is accepted in the housing. The reagent strip includes a protective film, a baseboard, and includes a sample absorbing pad, a colloidal gold pad, a nitrocellulose membrane, and a water absorbing pad, which are connected in sequence and arranged on the baseboard. The protective film is arranged on one side of the nitrocellulose membrane. The colloidal gold pad coats a clenobuterol hydrochloride antibody. The nitrocellulose membrane is provided with a test line and a quality control line, wherein the test line coats a clenobuterol hydrochloride protein conjugate and is arranged near the colloidal gold pad. The quality control pad coats sheep or rabbit anti-mouse antibody and is near the water absorbing pad. The novel reagent testing card of clenobuterol hydrochloride effectively prevents air environmental factors against affecting color development of the test line, and has advantages of good test effect and quantitative test application.
Description
Technical field
Clenobuterol hydrochloride detection technique field of the present invention, is specifically related to a kind of new salts Clenbuterol and detects reagent card.
Background technology
Clenobuterol hydrochloride, has another name called for clenbuterol hydrochloride, is a kind of β of Prof. Du Yucang
2adrenoceptor agonists, is usually used in preventing and treating the pulmonary disease such as asthma and pulmonary emphysema.When its using dosage reaches the 5-10 times of therapeutic dose, have the effect regulating nutriment to flow to and redistribute, body muscle can be made to synthesize to be increased, and fat deposition reduces.If heavy dose of use can significantly improve feed conversion rate and lean meat percentage in husbandry sector, but also can cause the residual of clenbuterol hydrochloride in animal food simultaneously, toxicological effect can be produced to internal organs such as liver kidneys after edible, there is the symptoms such as palpitaition, headache, dizzy and muscle chatter, thus cause the generation of mankind's poisoning, even can cause death time serious.Therefore, countries in the world government generally all forbids with the feed addictive of clenobuterol hydrochloride as farming animals.Also clearly forbid adding clenobuterol hydrochloride in feed in No. 235 bulletin that the Ministry of Agriculture of China issued on Dec 24th, 2002, and be defined in Determination of Clenbuterol in animal food and must not be and detect.Therefore, effective detection method is one of key controlling clenobuterol hydrochloride abuse, is necessary the method studying high sensitivity Rapid Detection of Clenbuterol.
At present, the conventional method detecting clenobuterol hydrochloride mainly contains gas chromatography-mass spectrography, high performance liquid chromatography, euzymelinked immunosorbent assay (ELISA) and colloidal gold immunity chromatography etc.Wherein, colloidal gold immunity chromatography is theoretical based on immunochromatography technique, by preparing immune-gold labeled reagent card, the clenobuterol hydrochloride in animals urine or tissue samples being detected, qualitatively judging in animality meat product whether there is clenobuterol hydrochloride residue with this.This reagent card has easy and simple to handle, that detection is quick and expense is low advantage, and thus, detection or sample primary dcreening operation stage are widely used at the scene.
But, existing utilize the clenobuterol hydrochloride of colloidal gold immunity chromatography to detect reagent card to have detection sensitivity high and can not be applied to the shortcoming of qualitative detection device.Its reason is, because reagent card coloured moiety is directly exposed in air, thus very easily cause environmental factor on the impact of reagent card, thus cause the color of detection line on reagent card unstable, cause and rely on naked eyes to judge to occur that false positive and false-negative situation occur at profound look with time extremely light, cause erroneous judgement, thus affect the food security of the people, in addition, when needs quantitatively detect clenobuterol hydrochloride residue, due to the above-mentioned defect that current clenobuterol hydrochloride gold-immunochromatographyreagent reagent for assay card exists, cause environmental factor on the impact of reagent card detection line color, make instability and the aggravation of distortion situation of reagent card detection line color, can the effect of further magnification distortion when reading data based on the photoelectric sensor of this reagent card detection line color, finally be reflected in quantitative judgement numerically, serious inaccuracy will be there is, thus cause the clenobuterol hydrochloride generally used at present to detect reagent card and cannot be applied to quantitatively detection or qualitative detection poor effect.
Summary of the invention
Detect reagent card and exist to solve above-mentioned clenobuterol hydrochloride and be subject to the impact of air ambient factor and cause the color of detection line on reagent card unstable, thus do not cause Detection results good and the technical matters quantitatively detected cannot be applied to, the invention provides a kind of air ambient factor of can effectively avoiding to the chromogenic impact of detection line on reagent card, Detection results is good and can be applied to the new salts Clenbuterol quantitatively detected and detect reagent card.
The invention provides a kind of new salts Clenbuterol and detect reagent card, comprise the housing of reagent strip and tool receiving space, described reagent strip is contained in described housing, described reagent strip comprises diaphragm, base plate is also connected the sample absorbent of setting successively with being located at described base plate, colloidal gold pad, nitrocellulose filter and adsorptive pads, a side surface of described nitrocellulose filter is located at by described diaphragm, described colloidal gold pad is coated with anti-antibody of clenbuteral hydrochloride, described nitrocellulose filter is provided with detection line and nature controlling line, described detection line is coated with clenobuterol hydrochloride protein conjugate and arranges near described colloidal gold pad, described nature controlling line is coated with sheep or rabbit anti-mouse antibody also close described adsorptive pads.
Detect at new salts Clenbuterol provided by the invention in a kind of preferred embodiment of reagent card, described base plate is the back up pad that PVC material is made.
Detect at new salts Clenbuterol provided by the invention in a kind of preferred embodiment of reagent card, described sample absorbent comprises the groove being located at its one end, and described colloidal gold pad one end is located in the groove of described sample absorbent.
Detect at new salts Clenbuterol provided by the invention in a kind of preferred embodiment of reagent card, described colloidal gold pad comprises the slot being located at its one end, and described nitrocellulose filter one end is located in the slot of described colloidal gold pad.
Detect at new salts Clenbuterol provided by the invention in a kind of preferred embodiment of reagent card, described adsorptive pads comprises the socket being located at its one end, and the described nitrocellulose filter other end is located in the socket of described adsorptive pads.
Detect at new salts Clenbuterol provided by the invention in a kind of preferred embodiment of reagent card, described housing is one end open and the bar shaped housing of inner hollow, and described openend is used for insertion and the taking-up of described reagent strip; Described housing comprises droplet sample window and the display window that run through one side surface and arrange, and described sample window is just arranged described sample absorbent, and described display window is just arranged described nitrocellulose filter.
Detect at new salts Clenbuterol provided by the invention in a kind of preferred embodiment of reagent card, described housing also comprises the cutting ferrule being located at its outside surface, for depositing the card comprising sample information.
Detect in a kind of preferred embodiment of reagent card at new salts Clenbuterol provided by the invention, described in comprise sample information card be bar code or Quick Response Code card.
Detect at new salts Clenbuterol provided by the invention in a kind of preferred embodiment of reagent card, described diaphragm is the anti-oxidation polyethylene protective film of air insulated.
Compared to prior art, new salts Clenbuterol provided by the invention detects reagent card and has following beneficial effect:
One, diaphragm is set up by the side surface at nitrocellulose filter, nitrocellulose filter is made can effectively to avoid air ambient factor to the chromogenic impact of detection line on nitrocellulose filter, thus making colour developing more stable, effect is better, conveniently accurately qualitatively judges testing result; And make clenobuterol hydrochloride detection reagent card can also be applied to quantitative detection, quantitatively judge data more true and reliable;
Setting up two, by diaphragm, make clenobuterol hydrochloride detection reagent card avoid the impact of environmental factor, the storage time is more of a specified duration, convenient transport and non-at-scenely provide result in real time, can concentrate the reagent card after detecting and preserve, unified Treatment Analysis, reduce workload, reduce human time's cost;
Three, have groove by sample absorbent is designed to one end, colloidal gold pad one end is located in the groove of sample absorbent; Colloidal gold pad is designed to one end and has slot, and nitrocellulose filter one end is located in the slot of colloidal gold pad; Adsorptive pads is designed to one end and has socket, and the nitrocellulose filter other end is located in the socket of adsorptive pads; Sample to be tested is instilled after sample absorbent, and sandwich design effectively promotes chromatographic effect, makes the chromatography of antigen, antibody and water, permeates and divide a word with a hyphen at the end of a line and have more advantage, significantly improve the accuracy of testing result;
Four, by housing outer surface design cutting ferrule, for depositing bar coded cards containing sample information or Quick Response Code card, convenient when pattern detection to the reading of sample information, make testing result and sample information accurately corresponding, reduce the risk of intersecting and judging;
Five, by the air insulated protection against oxidation film adopting the base plate made of PVC material and adopt polythene material to make, reduce the cost that clenobuterol hydrochloride detects reagent card, and improve durability and storage time.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the embodiment of the present invention, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings, wherein:
Fig. 1 is the outside drawing that new salts Clenbuterol provided by the invention detects reagent card;
Fig. 2 is the schematic cross-section that the Clenbuterol of new salts shown in Fig. 1 detects the reagent strip of reagent card;
Fig. 3 is the vertical view that the Clenbuterol of new salts shown in Fig. 2 detects the reagent strip of reagent card.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making other embodiments all obtained under creative work prerequisite, belong to the scope of protection of the invention.
See also Fig. 1, Fig. 2 and Fig. 3, wherein Fig. 1 is the outside drawing that new salts Clenbuterol provided by the invention detects reagent card; Fig. 2 detects the schematic cross-section of the reagent strip of reagent card for the Clenbuterol of new salts shown in Fig. 1; Fig. 3 detects the vertical view of the reagent strip of reagent card for the Clenbuterol of new salts shown in Fig. 2.Described new salts Clenbuterol detects the housing 13 that reagent card 1 comprises reagent strip 11 and tool receiving space, and described reagent strip 11 is contained in described housing 13.
Described reagent strip 11 comprises base plate 111 and sample absorbent 112, colloidal gold pad 113, nitrocellulose filter 114, adsorptive pads 115 and diaphragm 116.Described sample absorbent 112, colloidal gold pad 113, nitrocellulose filter 114 and adsorptive pads 115 connect successively and are located at described base plate 111, and a side surface of described nitrocellulose filter 114 is located at by described diaphragm 116.
The back up pad that described base plate 111 is made for PVC material, has preferably impermeable ability, buffer action and reflection action.
Described sample absorbent 112 comprises the groove 1121 being located at its one end, described colloidal gold pad 113 one end is located in the groove 1121 of described sample absorbent, by the design of described groove 1121, increase described sample absorbent 112 and the contact area of described colloidal gold pad 113, be conducive to application of sample sample and penetrate into described colloidal gold pad 113 by described sample absorbent 112 chromatography.Preferably, described sample absorbent 112 is glass fibre membrane, has the receptivity of preferably animals urine sample, feed sample and tissue samples, for the application of sample of sample to be tested.
Described colloidal gold pad 113 comprises the slot 1131 being located at its one end, described nitrocellulose filter 114 one end is located in the slot 1131 of described colloidal gold pad, by the design of described slot 1131, increase described colloidal gold pad 113 and the contact area of described nitrocellulose filter 114, be conducive to sample and penetrate into described nitrocellulose filter 114 by described colloidal gold pad 113 chromatography.Preferably, described colloidal gold pad 113 is the glass fibre membrane containing colloid gold particle, and its colloid gold particle pan coating has the antibody that can identify clenobuterol hydrochloride.
Described nitrocellulose filter 114 is containing detection line 1141 and nature controlling line 1143, described detection line 1141 is coated with clenobuterol hydrochloride protein conjugate, and arrange near described colloidal gold pad 113 side, described nature controlling line 1143 is coated with sheep or rabbit anti-mouse antibody is also arranged near described adsorptive pads 115 side, folic acid Clenbuterol whether is remained in order to qualitatively judge detection sample by described nitrocellulose filter 114, be specially: positive (+): only an aubergine band appears in nature controlling line 1143, occur without aubergine band in detection line 1141, positive findings shows, clenobuterol hydrochloride concentration in sample is more than threshold value (3ng/ml), negative (-): two aubergine bands occur, article one, be positioned at detection line 1141, another is positioned at nature controlling line 1143, negative findings shows, clenobuterol hydrochloride concentration in sample in threshold value (3ng/ml) below, the phenomenon of shade can be shown, within the observing time of regulation (within 1 month), no matter this colour band shade is all judged to be negative findings at the aubergine band of detection line 1141, invalid: aubergine band does not appear in nature controlling line 1143, prompting test crash or test paper lost efficacy.
Described adsorptive pads 115 comprises the socket 1151 being located at its one end, described nitrocellulose filter 114 other end is located in the socket 1151 of described adsorptive pads, by the design of described socket 1151, increase described adsorptive pads 115 and the contact area of described nitrocellulose filter 114, be conducive to moisture chromatography in sample and penetrate into described adsorptive pads 115.
Described diaphragm 116 is the anti-oxidation polyethylene protective film of air insulated, is located at the opposite flank of described nitrocellulose filter 114 and described base plate 111 surface of contact.It act as: make described nitrocellulose filter 114 can effectively avoid air ambient factor to the chromogenic impact of detection line 1141 on described nitrocellulose filter, thus make colour developing more stable, more lasting, effect is better, conveniently accurately qualitatively judge testing result, and make the term of validity significant prolongation of described reagent strip 11.
Described housing 13 is one end open and the bar shaped housing of inner hollow, and described openend is used for insertion and the taking-up of described reagent strip 11.Described housing 13 comprises droplet sample window 131 and the display window 133 that run through one side surface and arrange, and described sample window 131 is just arranged described sample absorbent 112, and described display window 133 is just arranged described nitrocellulose filter 114.Carry out application of sample operation by described sample window 131, observe testing result by described display window 133.
Described housing 13 also comprises the cutting ferrule 135 being located at its outside surface, for depositing the card comprising sample information.The described card comprising sample information is bar code or Quick Response Code card, the typing of convenience sample information and derivation.
Described new salts Clenbuterol detects the preparation method of reagent card 1:
1) experimental apparatus and reagent:
PVC board, glass fibre membrane, nitrocellulose filter, cutting machine, desk centrifuge, vacuum freeze drier etc.; Clenobuterol hydrochloride standard items, sheep or rabbit anti-mouse antibody (Sigma company), anti-hydrochloric acid Ke Lunte wheel antibody and clenobuterol hydrochloride-bovine serum albumin conjugate (CLB-BSA) are by experiment synthesis, macrogol, polyglycol, glycocoll, bovine serum albumin, 1% gold chloride, 1% sodium citrate, Tris-HCL damping fluid (PH=7.5) and distilled water etc.
2) preparation of colloidal gold solution:
Get 500mL distilled water in reagent bottle, first drip the gold chloride of 2500 μ L, be heated to boiling, drip into 2500 μ L sodium citrates again, observe solution colour change, when it gradually becomes redness from aubergine, little fire heating, after 5-10 minute, cool, then it is stand-by to be stored in 4 DEG C of refrigerators.
3) preparation of colloidal gold pad:
Get 5mLTris-HCL damping fluid and the anti-hydrochloric acid Ke Lunte of 20 μ L to take turns antibody and mix and shake up, then add 50mL colloidal gold solution, in ice bath, place 5-10 minute afterwards, and mixed liquor PH is adjusted to more than 8.5; Leave standstill after 30-40 minute, continue to add 600 μ L bovine serum albumins, leave standstill 5-10 minute afterwards, be stored in centrifuge tube, and with 12000r/min centrifugal treating, produce red precipitate, then through cleansing solution washing 2-3 time, evenly dripped by red precipitate and make described colloidal gold pad in glass fibre membrane, freezen protective is stand-by;
CLB-BSA and sheep/rabbit anti-mouse igg antibody are sprayed on nitrocellulose filter, utilize vacuum freeze drier to carry out low-temperature vacuum drying, preserve stand-by.
4) encapsulation of reagent strip:
PVC base plate before installing preserve stand-by nitrocellulose filter, before described nitrocellulose filter is installed, it is first blockaded, utilize 25g/L bovine serum albumin and 1.5g glycocoll to realize, eliminate the situation of non-specific binding; Then the anti-oxidation polyethylene protective film of one deck air insulated is assembled in the opposite flank of nitrocellulose filter and described PVC base plate contact surface; Afterwards, more successively sample absorbent, colloidal gold pad and adsorptive pads is assembled; Finally, utilize cutting machine to carry out cutting processing, make the reagent strip of 6cm × 1cm, dry for subsequent use.
5) preparation of reagent card:
The one end open providing a collection of producer to customize and the bar shaped housing of inner hollow, hollow space size and described reagent strip size adapt, and inserted by described reagent strip and engage by the openend of housing; Described housing comprises droplet sample window and the display window that run through one side surface and arrange, described sample window is just arranged described sample absorbent, described display window is just arranged described nitrocellulose filter, finally carry out sealing packaging, so far described new salts Clenbuterol detection reagent card is prepared complete, be stored in low temperature seal environment, for subsequent use.
Described new salts Clenbuterol detects the using method of reagent card 1:
1) preparation of sample:
Urine specimen: a certain amount of livestock urine of random sampling, 3000r/min centrifugally operated 5 minutes, gets supernatant 100 μ L and detects, and detecting liquid extension rate is 1;
Feed sample: the feed sample of random sampling 2g homogeneity process, add the 0.1MHCL solution of 20ml, concussion operation 5 minutes, 3000r/mmin centrifugally operated 10 minutes, get the 1MNaOH solution that supernatant 2ml adds 40 μ L, 3000r/mmin centrifugally operated 10 minutes again, gets 100 μ L supernatants and detects, and detecting liquid extension rate is 10;
Tissue samples: random sampling 5g pulverizes tissue samples, adds the trichloroacetic acid of 12ml3%, concussion puts upside down mixing, 3000r/mmin centrifugally operated 10 minutes; Get supernatant 6ml and add 6ml isopropyl alcohol-ethyl acetate mixtures, vibrate 10 minutes, leave standstill 5 minutes; 3000r/mmin centrifugally operated 10 minutes again, gets 100 μ L supernatants and detects, and detecting liquid extension rate is 5;
2) application of sample detects:
The new salts Clenbuterol prepared is detected reagent card take out, under room temperature environment, 25 DEG C-35 DEG C are carried out pattern detection;
Clenobuterol hydrochloride titer 0ng/ml, 1ng/ml, 3ng/ml, 5ng/ml, 7ng/ml, 9ng/ml are dripped respectively the sample window in reagent card, carry out color observation as a control group; Sample to be tested is dripped respectively sample in a sample window of reagent card, each sample window drips 3 sample to be tested liquid successively;
3) result judges:
Color observation is carried out by the display window of reagent card, initial characterization judgement is carried out to the color of sample to be tested test card, whether to determine in sample to be tested containing clenobuterol hydrochloride residue, be specially: positive (+): only an aubergine band appears in nature controlling line 1143, occur without aubergine band in detection line 1141, positive findings shows, the clenobuterol hydrochloride concentration in sample is more than threshold value (3ng/ml); Negative (-): two aubergine bands occur, article one, be positioned at detection line 1141, another is positioned at nature controlling line 1143, negative findings shows, clenobuterol hydrochloride concentration in sample in threshold value (3ng/ml) below, the phenomenon of shade can be shown, within the observing time of regulation (within 1 month), no matter this colour band shade is all judged to be negative findings at the aubergine band of detection line 1141; Invalid: aubergine band does not appear in nature controlling line 1143, prompting test crash or test paper lost efficacy.
In addition, also by comparing with the color of control group, carrying out further roughly rational judgment, understanding the estimation numerical value of sample to be tested clenobuterol hydrochloride residue.
4) test analysis:
Utilize described new salts Clenbuterol to detect reagent card 1 and detect sample to be tested, the recovery of clenobuterol hydrochloride is 90.5%-96.5%, as shown in the table.Therefore, it is higher that described new salts Clenbuterol detects reagent card 1 accuracy of detection, can meet the extensive testing requirement of actual sample.
The determination of recovery rates table of table 1 clenobuterol hydrochloride
In addition, test discovery further, the colour band after described new salts Clenbuterol detects reagent card 1 application of sample can keep constant for a long time, and most long storage life can reach 1 month, and colour band color is without significant change.
Clenobuterol hydrochloride provided by the invention detects reagent card and has following beneficial effect:
One, diaphragm is set up by the side surface at nitrocellulose filter, nitrocellulose filter is made can effectively to avoid air ambient factor to the chromogenic impact of detection line on nitrocellulose filter, thus making colour developing more stable, effect is better, conveniently accurately qualitatively judges testing result; And make clenobuterol hydrochloride detection reagent card can also be applied to quantitative detection, quantitatively judge data more true and reliable;
Setting up two, by diaphragm, make clenobuterol hydrochloride detection reagent card avoid the impact of environmental factor, the storage time is more of a specified duration, convenient transport and non-at-scenely provide result in real time, can concentrate the reagent card after detecting and preserve, unified Treatment Analysis, reduce workload, reduce human time's cost;
Three, have groove by sample absorbent is designed to one end, colloidal gold pad one end is located in the groove of sample absorbent; Colloidal gold pad is designed to one end and has slot, and nitrocellulose filter one end is located in the slot of colloidal gold pad; Adsorptive pads is designed to one end and has socket, and the nitrocellulose filter other end is located in the socket of adsorptive pads; Sample to be tested is instilled after sample absorbent, and sandwich design effectively promotes chromatographic effect, makes the chromatography of antigen, antibody and water, permeates and divide a word with a hyphen at the end of a line and have more advantage, significantly improve the accuracy of testing result;
Four, by housing outer surface design cutting ferrule, for depositing bar coded cards containing sample information or Quick Response Code card, convenient when pattern detection to the reading of sample information, make testing result and sample information accurately corresponding, reduce the risk of intersecting and judging;
Five, by the air insulated protection against oxidation film adopting the base plate made of PVC material and adopt polythene material to make, reduce the cost that clenobuterol hydrochloride detects reagent card, and improve durability and storage time.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize instructions of the present invention and accompanying drawing content to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical field, be all in like manner included in scope of patent protection of the present invention.
Claims (9)
1. a new salts Clenbuterol detects reagent card, comprise the housing of reagent strip and tool receiving space, described reagent strip is contained in described housing, described reagent strip comprises base plate and is located at described base plate and is connected the sample absorbent of setting successively, colloidal gold pad, nitrocellulose filter and adsorptive pads, it is characterized in that, described reagent strip also comprises diaphragm, a side surface of described nitrocellulose filter is located at by described diaphragm, described colloidal gold pad is coated with anti-antibody of clenbuteral hydrochloride, described nitrocellulose filter is provided with detection line and nature controlling line, described detection line is coated with clenobuterol hydrochloride protein conjugate and arranges near described colloidal gold pad, described nature controlling line is coated with sheep or rabbit anti-mouse antibody also close described adsorptive pads.
2. new salts Clenbuterol according to claim 1 detects reagent card, and it is characterized in that, described base plate is the back up pad that PVC material is made.
3. new salts Clenbuterol according to claim 1 detects reagent card, and it is characterized in that, described sample absorbent comprises the groove being located at its one end, and described colloidal gold pad one end is located in the groove of described sample absorbent.
4. new salts Clenbuterol according to claim 1 detects reagent card, and it is characterized in that, described colloidal gold pad comprises the slot being located at its one end, and one end of described nitrocellulose filter is located in the slot of described colloidal gold pad.
5. new salts Clenbuterol according to claim 4 detects reagent card, and it is characterized in that, described adsorptive pads comprises the socket being located at its one end, and the other end of described nitrocellulose filter is located in the socket of described adsorptive pads.
6. new salts Clenbuterol according to claim 1 detects reagent card, and it is characterized in that, described housing is one end open and the bar shaped housing of inner hollow, and described openend is used for insertion and the taking-up of described reagent strip; Described housing comprises droplet sample window and the display window that run through one side surface and arrange, and described sample window is just arranged described sample absorbent, and described display window is just arranged described nitrocellulose filter.
7. new salts Clenbuterol according to claim 6 detects reagent card, and it is characterized in that, described housing also comprises the cutting ferrule being located at its outside surface, for depositing the card comprising sample information.
8. new salts Clenbuterol according to claim 7 detects reagent card, it is characterized in that, described in comprise sample information card be bar code or Quick Response Code card.
9. new salts Clenbuterol according to claim 1 detects reagent card, and it is characterized in that, described diaphragm is the anti-oxidation polyethylene protective film of air insulated.
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| CN201510416606.8A CN105044366A (en) | 2015-07-15 | 2015-07-15 | Novel reagent testing card of clenobuterol hydrochloride |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442976A (en) * | 2016-08-31 | 2017-02-22 | 杭州泰熙生物技术有限公司 | Method for making clenbuterol hydrochloride detection kit |
| CN107132356A (en) * | 2017-04-27 | 2017-09-05 | 北京航空航天大学 | A kind of colloidal gold test paper card for detecting carcinoma of urinary bladder and preparation method thereof |
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| CN106442976A (en) * | 2016-08-31 | 2017-02-22 | 杭州泰熙生物技术有限公司 | Method for making clenbuterol hydrochloride detection kit |
| CN106442976B (en) * | 2016-08-31 | 2018-08-03 | 杭州泰熙生物技术有限公司 | The production method that clenobuterol hydrochloride detects box |
| CN107132356A (en) * | 2017-04-27 | 2017-09-05 | 北京航空航天大学 | A kind of colloidal gold test paper card for detecting carcinoma of urinary bladder and preparation method thereof |
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