CN111707819B - AFB1, ZEN and DON three-index quantitative chromatographic test strip - Google Patents
AFB1, ZEN and DON three-index quantitative chromatographic test strip Download PDFInfo
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- CN111707819B CN111707819B CN202010575359.7A CN202010575359A CN111707819B CN 111707819 B CN111707819 B CN 111707819B CN 202010575359 A CN202010575359 A CN 202010575359A CN 111707819 B CN111707819 B CN 111707819B
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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Abstract
The invention provides a AFB, ZEN, DON three-index quantitative chromatographic test strip, and relates to the technical field of reagent detection. This AFB, ZEN, DON three index ration chromatographic test strip, including bottom welt, one side at bottom welt top is provided with the sample pad, the center at bottom welt top is provided with the bottom chromatographic membrane, the top fixedly connected with middle layer chromatographic membrane of bottom chromatographic membrane, the top fixedly connected with top layer chromatographic membrane of middle layer chromatographic membrane, one side fixedly connected with that the sample pad was kept away from at the top of bottom welt is with the corresponding pad that absorbs water of bottom chromatographic membrane, middle layer chromatographic membrane and top layer chromatographic membrane, bottom chromatographic membrane, middle layer chromatographic membrane and top layer chromatographic membrane are kept away from one side that the colloidal gold combined pad all is provided with the quality control line. Through designing bottom chromatographic membrane, middle layer chromatographic membrane and top layer chromatographic membrane, can carry out short-term test to aflatoxin B1 (AFB 1), zearalenone (ZEN) and vomitoxin (DON), convenient and fast is worth popularizing greatly.
Description
Technical Field
The invention relates to the technical field of reagent detection, in particular to an AFB1, ZEN and DON three-index quantitative chromatographic test strip.
Background
Aflatoxin B1 (abbreviated as AFB 1) is a derivative of dihydrofuran oxanaphthalene ortho-ketone, contains a double furan ring and an oxanaphthalene ortho-ketone (coumarin), aflatoxin B1 is one of the most cancerogenic of known chemical substances, aflatoxin B1 has strong toxicity to people and a plurality of animals, the toxic effect is mainly damage to liver, most of aflatoxin B1 is seen in natural food, the harmfulness is also the most strong, and the national general administration for quality inspection stipulates that aflatoxin B1 is one of the necessary inspection items of most foods; corn gibberellin (ZEN) mainly pollutes corn, wheat, rice, barley, millet, oat and other grains, wherein the positive detection rate of the corn is 45%, and the highest toxic content can reach 2909mg/kg; the detection rate of wheat is 20%, the toxic content is 0.364-11.05 mg/kg, the heat resistance of zearalenone is high, the zearalenone is completely destroyed after being treated for 1h at 110 ℃, the zearalenone has estrogen-like effect, can cause acute and chronic poisoning of animals, cause abnormal animal reproductive function and even death, can cause great economic loss to animal farm, and is a metabolite of zearalenone; vomitoxin (DON), also known as Deoxynivalenol (DON), is known as 3 alpha, 7 alpha, 15-trihydroxy Fusarium graminearum-9-en-8-one, belongs to a trichothecene compound, has a certain harm effect on human bodies because of being capable of causing vomiting of pigs, and has a three-level carcinogen as a European classification standard.
For the detection of aflatoxin B1 (AFB 1), zearalenone (ZEN) and vomitoxin (DON), the traditional detection mode adopts different detection test strips to detect separately, and the detection is carried out by adopting the different detection test strips, so that the time and the labor are wasted, the operation and the carrying are very inconvenient, and the detection efficiency and the accuracy are low, therefore, a new AFB1, ZEN and DON quantitative chromatographic test strip with three indexes is developed.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides the AFB1, ZEN and DON quantitative chromatographic test strip, which solves the problems that the traditional detection mode adopts different detection test strips to detect separately, the detection is time-consuming and labor-consuming, the operation and the carrying are very inconvenient, and the detection efficiency and the accuracy are low.
(II) technical scheme
In order to achieve the above purpose, the invention is realized by the following technical scheme: the utility model provides a AFB, ZEN, DON three index ration chromatography test strip, includes bottom welt, one side at bottom welt top is provided with the sample pad, the center at bottom welt top is provided with the bottom chromatographic membrane, the top fixedly connected with middle layer chromatographic membrane of bottom chromatographic membrane, the top fixedly connected with top layer chromatographic membrane of middle layer chromatographic membrane, one side of sample pad is provided with the corresponding colloidal gold bond pad with bottom chromatographic membrane, middle layer chromatographic membrane and surface layer chromatographic membrane, one side fixedly connected with that the sample pad was kept away from at the top of bottom welt is corresponding with bottom chromatographic membrane, middle layer chromatographic membrane and surface layer chromatographic membrane absorbs water to fill up, one side that the colloidal gold bond pad was kept away from to bottom chromatographic membrane, middle layer chromatographic membrane and surface layer chromatographic membrane all is provided with the quality control line.
Preferably, the bottom liner is made of PVC material.
Preferably, the bottom chromatographic membrane, the middle chromatographic membrane and the surface chromatographic membrane are fixedly connected through pressing.
Preferably, the absorbent pad is made of absorbent cotton material.
Preferably, a first detection line corresponding to the quality control line is arranged on one side of the bottom chromatographic membrane.
Preferably, a second detection line corresponding to the quality control line is arranged at the center of the middle chromatographic film.
Preferably, a third detection line corresponding to the quality control line is arranged on one side of the surface chromatographic membrane.
Preferably, a plurality of quality control lines are all arranged in an overlapping structure, and a plurality of quality control lines are all positioned on the same vertical straight line.
Working principle: when the AFB1, ZEN and DON three-index quantitative chromatographic test strip is used, a sample can be mixed and dripped on the sample pad 2, the bottom layer chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5 are formed by pressing, so that the fixed connection function can be achieved, the whole volumes of the bottom layer chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5 can be reduced, the water absorption pad 7 corresponding to the bottom layer chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5 is fixedly connected to one side of the top of the bottom lining plate 1, which is far away from the sample pad 2, the water absorption pad 7 made of a water absorption cotton material has a better water absorption function, and therefore, the mixed liquid dripped on the sample pad 2 can be uniformly absorbed in the bottom layer chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5 under the water absorption function of the water absorption pad 7, a first detection line 8 corresponding to a quality control line 11 is arranged on one side of the bottom chromatographic membrane 3, the bottom chromatographic membrane 3 and the first detection line 8 are used for detecting aflatoxin B1 (AFB 1), when the mixed solution contains the aflatoxin B1, the first detection line 8 turns red, so that the effect of rapid detection of the aflatoxin B1 (AFB 1) is realized, a second detection line 9 corresponding to the quality control line 11 is arranged at the center of the middle chromatographic membrane 4, the middle chromatographic membrane 4 and the second detection line 9 are used for detecting Zearalenone (ZEN), when the mixed solution contains the Zearalenone (ZEN), the second detection line 9 turns red, so that the effect of rapid detection of the Zearalenone (ZEN) is realized, a third detection line 10 corresponding to the quality control line 11 is arranged on one side of the surface chromatographic membrane 5, the surface chromatographic membrane 5 and the third detection line 10 are used for detecting vomitoxin (DON), when the mixed solution contains vomitoxin (DON), the third detection line 10 turns red, so that the effect of rapid detection of vomitoxin (DON) is realized, the multiple quality control lines 11 arranged in an overlapping structure can turn red after adsorbing the mixed solution to be detected, and the multiple quality control lines 11 positioned on the same vertical straight line can turn red after adsorbing the mixed solution containing aflatoxin B1 (AFB 1), zearalenone (ZEN) or vomitoxin (DON), so that the detection effectiveness is ensured.
(III) beneficial effects
The invention provides an AFB1, ZEN and DON three-index quantitative chromatographic test strip. The beneficial effects are as follows:
1. the AFB1, ZEN and DON quantitative chromatographic test strip can utilize the same test strip to complete the rapid detection of different toxins through a simple combined sample rapid detection structure, is convenient to operate and carry, and improves the detection efficiency.
2. According to the AFB1, ZEN and DON quantitative chromatographic test strip, the bottom chromatographic membrane, the middle chromatographic membrane and the surface chromatographic membrane are designed, so that aflatoxin B1 (AFB 1), zearalenone (ZEN) and vomit toxin (DON) can be rapidly detected, and the rapid and convenient detection is realized, so that the working efficiency of the rapid and convenient quantitative chromatographic test strip is greatly improved, and the rapid and convenient quantitative test strip is worthy of being widely popularized.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic top view of a chromatographic carrier according to the invention;
FIG. 3 is a schematic front view of the chromatographic carrier according to the invention.
Wherein, 1, a bottom lining plate; 2. a sample pad; 3. a bottom chromatographic membrane; 4. a middle layer chromatographic membrane; 5. a surface layer chromatographic membrane; 6. a colloidal gold bonding pad; 7. a water absorbing pad; 8. a first detection line; 9. a second detection line; 10. a third detection line; 11. and a quality control line.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples:
as shown in fig. 1-3, the embodiment of the invention provides an AFB1, ZEN and DON three-index quantitative chromatographic test strip, which comprises a bottom liner plate 1, wherein a sample pad 2 is arranged on one side of the top of the bottom liner plate 1, a bottom chromatographic membrane 3 is arranged in the center of the top of the bottom liner plate 1, a middle layer chromatographic membrane 4 is fixedly connected to the top of the bottom chromatographic membrane 3, a surface layer chromatographic membrane 5 is fixedly connected to the top of the middle layer chromatographic membrane 4, a colloidal gold bonding pad 6 corresponding to the bottom chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5 is arranged on one side of the sample pad 2, a water absorbing pad 7 corresponding to the bottom layer chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5 is fixedly connected to one side of the top of the bottom liner plate 1, and one sides of the bottom layer chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5, which are away from the colloidal gold bonding pad 6, and a quality control line 11 is arranged.
The bottom lining board 1 is made of PVC material, and the bottom lining board 1 made of PVC material not only can play a role in supporting and fixing, but also can enable the structure of the test strip to be firmer and stretch-proof.
The bottom chromatographic membrane 3, the middle chromatographic membrane 4 and the surface chromatographic membrane 5 are fixedly connected through pressing, and the bottom chromatographic membrane 3, the middle chromatographic membrane 4 and the surface chromatographic membrane 5 are formed through pressing, so that the fixed connection function can be achieved, and meanwhile, the whole volumes of the bottom chromatographic membrane 3, the middle chromatographic membrane 4 and the surface chromatographic membrane 5 can be reduced.
The water absorption pad 7 is made of water absorption cotton material, and the water absorption pad 7 made of water absorption cotton material has better water absorption effect, so that the mixed liquid dropped on the sample pad 2 can be uniformly absorbed in the bottom chromatographic membrane 3, the middle chromatographic membrane 4 and the surface chromatographic membrane 5 under the water absorption effect of the water absorption pad 7.
One side of the bottom chromatographic membrane 3 is provided with a first detection line 8 corresponding to a quality control line 11, the bottom chromatographic membrane 3 and the first detection line 8 are used for detecting aflatoxin B1 (AFB 1), and when the mixed solution contains aflatoxin B1, the first detection line 8 turns red, so that the effect of rapid detection of the aflatoxin B1 (AFB 1) is realized.
The center of the middle layer chromatographic membrane 4 is provided with a second detection line 9 corresponding to a quality control line 11, the middle layer chromatographic membrane 4 and the second detection line 9 are used for detecting Zearalenone (ZEN), and when the mixed solution contains Zearalenone (ZEN), the second detection line 9 turns red, so that the effect of rapidly detecting the Zearalenone (ZEN) is realized.
One side of the surface chromatographic film 5 is provided with a third detection line 10 corresponding to the quality control line 11, the surface chromatographic film 5 and the third detection line 10 are used for detecting vomitoxin (DON), and when the mixed solution contains the vomitoxin (DON), the third detection line 10 turns red, so that the effect of rapidly detecting the vomitoxin (DON) is realized.
The quality control lines 11 are all arranged in an overlapping structure, the quality control lines 11 are all located on the same vertical straight line, the quality control lines 11 arranged in the overlapping structure can turn red after adsorbing mixed liquid to be detected, and the quality control lines 11 located on the same vertical straight line can turn red no matter after adsorbing mixed liquid containing aflatoxin B1 (AFB 1), zearalenone (ZEN) or vomit toxin (DON), so that the detection effectiveness is ensured.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (2)
1. The utility model provides an AFB1, ZEN, DON three index quantitative chromatographic test strip, includes bottom welt (1), its characterized in that: the device is characterized in that a sample pad (2) is arranged on one side of the top of the bottom lining plate (1), a bottom chromatographic membrane (3) is arranged in the center of the top of the bottom lining plate (1), a middle layer chromatographic membrane (4) is fixedly connected to the top of the bottom chromatographic membrane (3), a surface layer chromatographic membrane (5) is fixedly connected to the top of the middle layer chromatographic membrane (4), a colloidal gold bonding pad (6) corresponding to the bottom chromatographic membrane (3), the middle layer chromatographic membrane (4) and the surface layer chromatographic membrane (5) is arranged on one side of the sample pad (2), a water absorbing pad (7) corresponding to the bottom chromatographic membrane (3), the middle layer chromatographic membrane (4) and the surface layer chromatographic membrane (5) is fixedly connected to one side of the top of the bottom lining plate (1) away from the sample pad (2), and quality control lines (11) are arranged on one sides of the bottom chromatographic membrane (3), the middle layer chromatographic membrane (4) and the surface layer chromatographic membrane (5) away from the colloidal gold bonding pad (6);
the bottom lining board (1) is made of PVC material;
the bottom chromatographic membrane (3), the middle chromatographic membrane (4) and the surface chromatographic membrane (5) are fixedly connected through pressing;
the water absorption pad (7) is made of a water absorption cotton material;
a first detection line (8) corresponding to the quality control line (11) is arranged on one side of the bottom chromatographic membrane (3);
the center of the middle chromatographic membrane (4) is provided with a second detection line (9) corresponding to the quality control line (11);
one side of the surface chromatographic membrane (5) is provided with a third detection line (10) corresponding to the quality control line (11).
2. The AFB1, ZEN and DON three-index quantitative chromatographic test strip according to claim 1, wherein the test strip is characterized in that: the quality control lines (11) are all arranged in an overlapping structure, and the quality control lines (11) are all positioned on the same vertical straight line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202010575359.7A CN111707819B (en) | 2020-06-22 | 2020-06-22 | AFB1, ZEN and DON three-index quantitative chromatographic test strip |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010575359.7A CN111707819B (en) | 2020-06-22 | 2020-06-22 | AFB1, ZEN and DON three-index quantitative chromatographic test strip |
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| CN111707819A CN111707819A (en) | 2020-09-25 |
| CN111707819B true CN111707819B (en) | 2023-07-21 |
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| JP2009216696A (en) * | 2008-02-12 | 2009-09-24 | Fujifilm Corp | Measurement kit crossed with developing directions of two developing liquids and allowing development from different directions, and immunochromatography method |
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| JP5610389B2 (en) * | 2010-11-05 | 2014-10-22 | 国立大学法人北陸先端科学技術大学院大学 | Immunochromatographic analysis strip and immunochromatographic analysis method |
| CN204330774U (en) * | 2015-01-15 | 2015-05-13 | 福建出入境检验检疫局检验检疫技术中心 | Bacillus tuberculosis bovis immunochromatographydetecting detecting test strip |
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| CN205353089U (en) * | 2015-11-18 | 2016-06-29 | 深圳市通量检测科技有限公司 | Detection apparatus for clenobuterol hydrochloride |
| CN205384281U (en) * | 2015-11-30 | 2016-07-13 | 江苏衡谱分析检测技术有限公司 | A trapezoidal chromatography test paper strip for detecting multicomponent pesticide residue |
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