CN104142398A - Colloidal gold test card for detection of rhodamine B and preparation method and application thereof - Google Patents
Colloidal gold test card for detection of rhodamine B and preparation method and application thereof Download PDFInfo
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- CN104142398A CN104142398A CN201310165213.5A CN201310165213A CN104142398A CN 104142398 A CN104142398 A CN 104142398A CN 201310165213 A CN201310165213 A CN 201310165213A CN 104142398 A CN104142398 A CN 104142398A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Abstract
The invention discloses a colloidal gold test card for detection of rhodamine B, a preparation method thereof and an application method thereof in detection of the rhodamine B, and belongs to the field of food safety detection. The detection card is capable of simple, rapid, specific and sensitive detection of the rhodamine B in food samples.
Description
Technical field
The present invention relates to the rapid detection technical field of food security, particularly, the present invention relates to the preparation method of the colloidal-gold detecting-card of a kind of fast detecting rhodamine B, the colloidal-gold detecting-card making thus and application.
Background technology
Rhodamine B (Rhodamine B) claim again rose red b or basic rhodamine, is commonly called as pollen red, is a kind of fresh pinkish artificial synthetic dyestuffs that have.Rhodamine B has strong fluorescence in solution, as industries such as cell fluorescence coloring agent, coloured glass, characteristic fireworks and firecrackers in laboratory.
Rhodamine B has fat-soluble, is once used as food dyeing and adjuvant, is mainly the coloring agent of paprika and chilli oil.Although country forbids adding rhodamine B in food, from 2011, a lot of rhodamine B food safety affairs have been there are.While having used contaminated flavouring to make food, can cause residually, through evidence rhodamine B, there is potential carcinogenic and mutagenicity.
Therefore, be necessary the rhodamine B in food to detect.Adopting more method is at present high performance liquid chromatography, the method has good sensitivity and specificity, but complex operation, length consuming time, be unsuitable for the detection to batch samples, and need expensive instrument and equipment, testing cost is high, and therefore need to provide a kind of can detect the method for rhodamine B in food quick, easy and delicately.
Summary of the invention
The invention provides the preparation method of the colloidal-gold detecting-card of a kind of fast detecting rhodamine B, its preparation technology is simple, and cost is low, and the colloidal-gold detecting-card making can meet the needs of field quick detection, detect fast, easy to carry, simple to operate, without specific installation and instrument.In addition, the present invention also provides the method for utilizing colloidal-gold detecting-card of the present invention to detect rhodamine B.
On the one hand, the invention provides a kind of preparation method who detects the colloidal-gold detecting-card of rhodamine B, it comprises preparation gold mark pad 8, the preparation of described gold mark pad 8 comprises: with the gold mark albumen working fluid spraying gold mark pad containing the anti-rhodamine B monoclonal antibody of gold mark, it is for example SDS-L of volume ratio 1%～2%, for example SDS-L of volume ratio 1% of 0.5%～2% SDS-L(that wherein said gold mark albumen working fluid comprises volume ratio) and 0.02M phosphate buffer.
In one embodiment, described gold mark albumen working fluid also comprises 0.1 ‰ g/ml PVA, 0.1 ‰ g/ml BSA, 0.3 ‰ g/ml PEG20000 and 5%g/ml sucrose.
In one embodiment, the preparation of the anti-rhodamine B monoclonal antibody of described gold mark comprises:
A. use 0.01%g/ml aqueous solution of chloraurate and 1%g/ml sodium citrate aqueous solution to prepare colloidal gold solution, wherein 0.01%g/ml aqueous solution of chloraurate: 1%g/ml sodium citrate=(10～1000) mL:(0.2～20) mL;
B. use prepared colloidal gold solution mark rhodamine B monoclonal antibody, the colloidal gold solution that wherein used: 0.75mg/mL rhodamine B monoclonal antibody: 1%g/ml PEG20000:10%g/ml BSA=(10～1000) mL:(150～15000) μ g:(1～100) mL:(1～100) mL.
Particularly, according to the consumption of the anti-rhodamine B monoclonal antibody working fluid of the corresponding gold mark of 0.8cm*30cm gold mark pad (the anti-rhodamine B monoclonal antibody of concentrated gold mark is pressed the gold mark albumen working fluid dilution for volume ratio of 1:2～1:5) 0.7mL, with 1mL liquid-transfering gun, gold being marked to anti-rhodamine B monoclonal antibody working fluid, to be evenly sprayed at gold mark pad upper, is placed in after 35 ℃ of vacuum drying standby.
In one embodiment, 0.01%g/ml aqueous solution of chloraurate in colloidal gold solution preparation: 1%g/ml sodium citrate=100mL:2mL, with the colloidal gold solution using in prepared colloidal gold solution mark rhodamine B monoclonal antibody: 0.75mg/mL rhodamine B monoclonal antibody: 1%g/ml PEG20000:10%g/ml BSA=100mL:1.5mg:10mL:10mL.
In one embodiment, described method also comprises prepares nitrocellulose filter 7, the preparation of described nitrocellulose filter 7 is included in and on nitrocellulose filter, sprays rhodamine B antigenic solution and sheep anti-mouse igg solution, form two parallel lines as detection line 10 and nature controlling line 11, wherein rhodamine B antigenic solution is for containing the phosphate buffer of the 0.02M that the pH of rhodamine B antigen is 7.2, and the volume of 10.7mg/mL rhodamine B antigen: the volume of the phosphate buffer of 0.02M is 1:30 to 1:42, 1:35 for example, the 0.02M phosphate buffer that sheep anti-mouse igg solution is 7.2 for the pH containing sheep anti-mouse igg, and the volume of the phosphate buffer of the volume of 7.5mg/mL sheep anti-mouse igg: 0.02M is 1:3 to 1:5, 1:4 for example.
In one embodiment, described method also comprises preparation sample pad 9, and the preparation of described sample pad 9 comprises the 0.05M Tris damping fluid processing sample pad with pH7.4.
In one embodiment, described method also comprises preparation
Thieving paper 6;
Thieving paper 6, nitrocellulose filter 7, gold mark pad 8, sample pad 9 are sticked on base plate 5, form gold label test strip 2;
Gold label test strip 2 is packed in test card housing 1, make the position of sample pad 9 over against the well 3 of test card housing 1, the position of nitrocellulose filter 7 is over against the observation port 4 of test card housing 1.
On the other hand, the invention provides the colloidal-gold detecting-card of the rhodamine B of being prepared by method of the present invention.
Again on the one hand, the invention provides the method that detects the rhodamine B in sample with rhodamine B colloidal-gold detecting-card of the present invention, described method comprises carries out pre-treatment to sample.
In one embodiment, the pre-treatment of described sample comprises:
Take 1g sample, in 50mL centrifuge tube, add 5～10mL acetonitrile, vortex vibration disperses sample completely, add 5mL normal hexane, vortex vibration mixes sample completely again, and 15～30min vibrates under room temperature, after centrifugal, get 1～2mL supernatant, nitrogen dries up, and then adds 1～2mL sample diluting liquid; Vortex vibrates until residual substance fully dissolves.
In detection method of the present invention, result of determination is as follows:
Negative: when Quality Control district shows a red stripes, detection zone also demonstrates a red stripes simultaneously, rapid detection card be take demonstration red line as " ︳ ︳ ", is judged to feminine gender;
Positive: when Quality Control district shows a red stripes, and do not develop the color in detection zone, rapid detection card be take and shown that red line is " ︳ ", is judged to the positive.
Invalid: when Quality Control district does not show red stripes, no matter whether detection zone demonstrates red stripes, and this test card is invalid.
In one embodiment, for detection of sample be thick broad-bean sauce, thick chilli sauce, paprika, chilli oil, chafing dish bottom flavorings.
Test card of the present invention has the following advantages:
1) high specificity, susceptibility is high.This colloidal-gold detecting-card be take the monoclonal antibody of colloid gold label high-affinity and is prepared from as basis, and the two combines by physisorption.Colloid gold label is very little on the specificity of monoclonal antibody and affinity impact, and has higher mark rate.Therefore, test card has stronger specificity and higher susceptibility, detects minimum the limiting the quantity of of rhodamine B and can reach 5ppb.
2) easy, quick, ageing strong.Use colloidal-gold detecting-card, without any other reagent and instrument, can execute-in-place, only need 5～8min detection time, can meet the needs of field quick detection, and easy to carry, simple to operate.
3) result shows image, directly perceived, accurate.Test card is usingd and is shown that reddish brown colo(u)r streak " ︳ " and " ︳ ︳ " are as the testing result positive and negative marker, while showing a brownish red " ︳ " on coated film, be illustrated in and in test sample, contain rhodamine B, while showing two reddish brown colo(u)r streaks " ︳ ︳ ", be illustrated in test sample not containing rhodamine B.Result is judged image, directly perceived, accurate, simple and clear, is not prone to the artificial erroneous judgement such as false positive and false negative.
4) cost saving, applied widely, be convenient to promote.Use test card of the present invention, than declining to a great extent by the expense of instrumental analysis and ELISA kit, the preparation technology of its test card of the present invention is simple, and cost is low, and can preserve at normal temperatures.In addition, test card of the present invention applied widely, can meet different levels personnel needs, comprise laboratory inspection, customs inspection, inspection and quarantine department at different levels, processing enterprise and consumer, it has changed the limitation that must just can be detected by the professional of professional institution the detection of rhodamine B, easy to utilize, there are wide market outlook and obvious economical, societal benefits.
Accompanying drawing explanation
Fig. 1 is the colloidal-gold detecting-card schematic diagram that the present invention detects rhodamine B;
Fig. 2 is the structural representation that the present invention detects gold label test strip 5 described in the colloidal-gold detecting-card of rhodamine B;
Fig. 3 is the left view of Fig. 2.
Embodiment
Key reagents and equipment source
Rhodamine B complete antigen (10.7mg/mL) is purchased from Shanghai wheat bin Bioisystech Co., Ltd with the anti-mouse monoclonal antibody of rhodamine B (7.5mg/mL); Sheep anti-mouse igg is purchased from Shanghai Hong Ju Bioisystech Co., Ltd; Gold mark point sample instrument and cutting cutter are purchased from Jin Biao bio tech ltd, Shanghai.
Embodiment 1 detects the preparation of the colloidal-gold detecting-card of rhodamine B
100 colloidal-gold detecting-cards that detect rhodamine B of take are below example, have described its preparation method.
(1) preparation of nitrocellulose filter 7
Particularly, the 0.02M phosphate buffer dilution that is 7.2 by the volume ratio of 1:35 and 1:4 with pH respectively by rhodamine B antigen (rhodamine B complete antigen) and sheep anti-mouse igg, then with gold mark point sample instrument (particularly, rhodamine B antigenic solution is placed in to pump 3 mark T, sheep anti mouse solution is positioned over to pump 1 mark C, then select manually to pump into 50 μ L at every turn, discharge 50 μ L, the air in emptying pipe; Select operation, open simulation model, then move to film and locate; After determining the position of film, start line continuously, each black mark of rear use of having rule rule irregular place, breakpoint etc., each line keeps the position of film to fix) it is sprayed into respectively to two parallel lines in nitrocellulose filter (long 20～25mm, wide 300mm, thick about 0.3mm, every wide 3mm in the test card finally making,) on, wherein, rhodamine B antigen is as detection line, sheep anti-mouse igg is as nature controlling line, and two lines occupy nitrocellulose filter centre position and at a distance of about 5mm, is then placed in after 35 ℃ of vacuum drying standby.
(2) preparation of sample pad 9
Particularly, according to the consumption of the 0.05M Tris damping fluid 5mL of the corresponding pH7.4 of 2.4cm*30cm sample pad, use the 0.05M Tris damping fluid that 1mL liquid-transfering gun is 7.4 by pH to be evenly sprayed at sample pad (glass fibre membrane, long 17mm, wide 300mm, thick about 0.5mm, every wide 3mm in the test card finally making) upper, be placed in after 25 ℃ of incubations standby.Or, can when treatment capacity is large, sample pad treating fluid be put into special stainless steel square box, sample pad is slowly immersed in treating fluid one by one, to liquid level higher than more than sample pad 3mm; Between sample pad, must not there be bubble; The sample pad number of plies must not be more than 20, are then placed in after 25 ℃ of incubations standby.
(3) preparation of colloidal gold solution
Particularly, the chlorauric acid solution with new system purified water preparation 0.01%g/ml, is placed in conical flask, and put into clean stirrer, with magnetic agitation electric jacket, be heated to boiling, under 150r/min magnetic agitation, the sodium citrate solution that adds rapidly 1%g/ml, keep temperature and rotating speed constant, while becoming shiny red to solution, continue to add after thermal agitation 15min, stop heating, and conical flask is removed from magnetic agitation electric jacket, room temperature is cooling, and 4 ℃ of Refrigerator stores are standby.Wherein, gold chloride: sodium citrate=100mL:2mL.
(4) by the anti-rhodamine B monoclonal antibody of colloidal gold solution mark preparing
Particularly, get the colloidal gold solution of above-mentioned preparation in centrifuge tube, with 0.1M solution of potassium carbonate, regulate pH to 7.4.When stirring, slowly add rhodamine B monoclonal antibody solution (monoclonal antibody of purchase adds with after 10 times of purified water dilutions, and in colloidal gold solution, antibody final concentration is 15 μ g/mL), after all adding and mixing, room temperature is placed 30min.Then the PEG20000 solution that slowly adds 1%g/ml, mixes rear room temperature and places 15min.Then slowly add 10%g/ml BSA solution with saturated free collaurum, then in 4 ℃, the centrifugal 30min of 10000rpm.Centrifugal rear taking-up centrifuge tube, careful supernatant discarded, after precipitating and dissolving with 1/10 volume (centrifugal front colloidal gold solution 1/10) gold mark albumen storage liquid (containing the aqueous solution of 0.1 ‰ g/ml PVA, 0.1 ‰ g/ml BSA, 0.3 ‰ g/ml PEG20000 and 5%g/ml sucrose), 4 ℃ of Refrigerator stores are standby.Wherein, colloidal gold solution: rhodamine B monoclonal antibody: PEG20000:BSA=100mL:1.5mg:10mL:10mL.
(5) preparation of gold mark pad 8
Particularly, according to the anti-rhodamine B monoclonal antibody working fluid of the corresponding gold mark of 0.8cm*30cm gold mark pad, (the anti-rhodamine B monoclonal antibody of concentrated gold mark of above-mentioned preparation is pressed to the gold mark albumen working fluid dilution for volume ratio of 1:4, gold mark albumen working fluid is 0.1 ‰ g/ml PVA, 0.1 ‰ g/ml BSA, 0.3 ‰ g/ml PEG20000, 5%g/ml sucrose and volume ratio are 1% SDS-L, pH is 7.4 0.02M phosphate buffer) consumption of 0.7mL, with 1mL liquid-transfering gun, gold is marked to anti-rhodamine B monoclonal antibody working fluid and be evenly sprayed at gold mark pad (polyester film, long 7～9mm, wide 300mm, thick about 0.5mm, every wide 3mm in the test card finally making) on, be placed in after 35 ℃ of vacuum drying standby.
(6) assembling of gold label test strip 2
Particularly, by base plate 5(PVC offset plate, upper current chart is believed Science and Technology Ltd., long 60mm, wide 300mm, thick about 0.5mm, every wide 3mm in the test card finally making) divide 4 positions from top to bottom, first dried nitrocellulose filter 7 is sticked on to relevant position, again respectively by thieving paper 6(thieving paper, be about 17mm, wide 300mm, thick about 0.5mm, every wide 3mm in the test card finally making) and dried gold mark pad 8 next-door neighbour's nitrocellulose filters 7 stick on base plate 5, and push down a little nitrocellulose filter 7, finally sample pad 9 next-door neighbour's gold mark pads 8 are attached on base plate 5, and push down gold mark and pad 8.With cutting cutter, assembled gold label test strip 2 is cut into 3mm width (the long 60mm of gold label test strip 2 after cutting, wide 3mm, thick about 1.5mm)
Pack the mark test strips 2 assembling into the long 70mm of test card housing 1(, wide 20mm, thick 5mm) in, now, the position of sample pad 9 is over against the well 3 of test card, and the position of nitrocellulose filter 7 is over against the observation port 4 of test card, pack into together with drying agent in aluminium foil bag, pack.
Specifically see Fig. 1 and Fig. 2.The colloidal-gold detecting-card of prepared rhodamine B, it comprises test card housing 1 and gold label test strip 2, has well 3 and observation port 4 on test card housing 1; Gold label test strip 2 is packaged in test card housing 1, gold label test strip 2 comprises base plate 5, on base plate 5, be disposed with thieving paper 6, nitrocellulose filter 7, gold mark pad 8 and sample pad 9, thieving paper 6 and gold mark pad 8 are separately positioned on nitrocellulose filter 7 two ends, junction, two ends at nitrocellulose filter 7, a bit of (1～2mm) pressed and overlapped of thieving paper 6 and gold mark pad 8 is on nitrocellulose filter 7, sample pad 9 is arranged on the other end of gold mark pad 8, and a bit of (1～2mm) pressed and overlapped of sample pad 9 is on gold mark pad 8; On gold mark pad 8, be coated with the anti-rhodamine B monoclonal antibody of gold mark, on nitrocellulose filter 7, being coated with successively material is the detection line 10 of rhodamine B antigen and the nature controlling line 11 that material is sheep anti-mouse igg.
The use of embodiment 2 rhodamine B test card
(1) Sample pretreatment:
Sample to be detected: thick broad-bean sauce, thick chilli sauce, paprika, chilli oil, chafing dish bottom flavorings etc.
Take 1g sample, in 50mL centrifuge tube, add 5mL acetonitrile, vortex vibration disperses sample completely, then adds 5mL normal hexane, and vortex vibration mixes sample completely, 15min vibrates under room temperature, after the centrifugal 5min of room temperature 4000rpm, get 1mL supernatant, nitrogen dries up, and then adds sample diluting liquid (the 0.01M phosphate buffer that pH is 7.4; Vortex vibration is redissolved residual substance, and sampling liquid detects.
(2) use test card
When sample is detected, test card is kept flat, get 80 μ L sample drops and enter well 3, observations in 5～8 minutes.
(3) Analysis of test results
Sheep anti-mouse igg and rhodamine B antigen are sprayed on respectively the nature controlling line 11(C of nitrocellulose filter) and detection line 10(T), testing sample containing rhodamine B moves to the other end according to chromatographic theory through sample pad 9, and successively through nature controlling line 11 and detection line 10, the anti-rhodamine B monoclonal antibody of golden mark being solidificated on gold mark pad 8 plays specific reaction with the rhodamine B in sample, and its rhodamine B on detection line of competition inhibition is combined.Therefore, when rhodamine B is when in sample, concentration is higher than a certain amount, the rhodamine B antibody of colloid gold label and the whole combinations of rhodamine B, thus in detection zone, because can not being combined with rhodamine B antigen, competitive reaction there will not be red stripes.In testing process, owing to lacking antibody antigen competitive reaction, will in detection zone and Quality Control district, there is red stripes in negative sample.
Negative: when Quality Control district shows a red stripes, detection zone also demonstrates a red stripes simultaneously, rapid detection card be take demonstration red line as " ︳ ︳ ", is judged to feminine gender, as shown in Fig. 1 (a).
Positive: when Quality Control district shows a red stripes, and do not develop the color in detection zone, rapid detection card be take and shown that red line is " ︳ ", is judged to the positive, as shown in Fig. 1 (b).
Invalid: when Quality Control district does not show red stripes, no matter whether detection zone demonstrates red stripes, and this test card is invalid, as shown in Fig. 1 (c).
After testing, the testing result of described thick broad-bean sauce, thick chilli sauce, paprika, chilli oil, chafing dish bottom flavorings is as follows.
| ? | Thick broad-bean sauce | Thick chilli sauce | Paprika | Chilli oil | Chafing dish bottom flavorings |
| Blank sample | - | - | - | - | - |
| Add 5ng/mL | + | + | + | + | + |
| Add 10ng/mL | + | + | + | + | + |
(4) impact of SDS-L on testing result in gold mark albumen working fluid
By 1:4, use respectively the anti-rhodamine B monoclonal antibody of the concentrated golden mark of gold mark albumen working fluid dilution of the SDS-L that containing SDS-L with containing volume ratio is not 0.5%, 1% and 2%, after being sprayed at gold mark pad, make test card, then for detection of testing sample, testing result is as shown in the table:
Claims (10)
1. a preparation method who detects the colloidal-gold detecting-card of rhodamine B, it comprises preparation gold mark pad (8), the preparation of described gold mark pad (8) comprising: with the gold mark albumen working fluid spraying gold mark pad containing the anti-rhodamine B monoclonal antibody of gold mark, it is 0.5%～2% SDS-L and the 0.02M phosphate buffer of pH7.4 that wherein said gold mark albumen working fluid comprises volume ratio.
2. method according to claim 1, it is 1%～2% SDS-L and the 0.02M phosphate buffer of pH7.4 that described gold mark albumen working fluid comprises volume ratio.
3. method according to claim 1 and 2, described gold mark albumen working fluid also comprises 0.1 ‰ g/ml PVA, 0.1 ‰ g/ml BSA, 0.3 ‰ g/ml PEG20000 and 5%g/ml sucrose.
4. according to the method in any one of claims 1 to 3, the preparation of the anti-rhodamine B monoclonal antibody of described gold mark comprises:
A. use 0.01%g/ml aqueous solution of chloraurate and 1%g/ml sodium citrate aqueous solution to prepare colloidal gold solution, wherein 0.01%g/ml aqueous solution of chloraurate: 1%g/ml sodium citrate=(10～1000) mL:(0.2～20) mL;
B. use prepared colloidal gold solution mark rhodamine B monoclonal antibody, the colloidal gold solution that wherein used: 0.75mg/mL rhodamine B monoclonal antibody: 1%g/ml PEG20000:10%g/ml BSA=(10～1000) mL:(150～15000) μ g:(1～100) mL:(1～100) mL.
5. according to the method described in any one in claim 1-4, wherein,
In colloidal gold solution preparation, 0.01%g/ml aqueous solution of chloraurate: 1%g/ml sodium citrate=100mL:2mL; With in prepared colloidal gold solution mark rhodamine B monoclonal antibody, the colloidal gold solution using: 0.75mg/mL rhodamine B monoclonal antibody: 1%g/ml PEG20000:10%g/ml BSA=100mL:1.5mg:10mL:10mL.
6. according to the method described in any one in claim 1-5, it also comprises prepares nitrocellulose filter (7), the preparation of described nitrocellulose filter (7) is included in and on nitrocellulose filter, sprays rhodamine B antigenic solution and sheep anti-mouse igg solution, form two parallel lines as detection line (10) and nature controlling line (11), the 0.02M phosphate buffer that wherein rhodamine B antigenic solution is 7.2 for the pH containing rhodamine B antigen, and the volume of 10.7mg/mL rhodamine B antigen: the volume of the phosphate buffer of 0.02M is 1:30 to 1:42, sheep anti-mouse igg solution is for containing the phosphate buffer of the 0.02M that the pH of sheep anti-mouse igg is 7.2, and the volume of the phosphate buffer of the volume of 7.5mg/mL sheep anti-mouse igg: 0.02M is 1:3 to 1:5.
7. method as claimed in claim 6, wherein the volume of the phosphate buffer of the volume of 10.7mg/mL rhodamine B antigen: 0.02M is 1:35, the volume of the phosphate buffer of the volume of 7.5mg/mL sheep anti-mouse igg: 0.02M is 1:4.
8. the method as described in aforementioned any one claim, it also comprises preparation sample pad (9), the preparation of described sample pad (9) comprises the 0.05M Tris damping fluid processing sample pad with pH7.4.
9. the method as described in aforementioned any one claim, it also comprises
Prepare thieving paper (6);
Thieving paper (6), nitrocellulose filter (7), gold mark pad (8), sample pad (9) are sticked on to base plate (5) above, form gold label test strip (2); With
Gold label test strip (2) is packed in test card housing (1), make the position of sample pad (9) over against the well (3) of test card housing (1), the position of nitrocellulose filter (7) is over against the observation port (4) of test card housing (1).
10. the colloidal-gold detecting-card of the rhodamine B that in claim 1-9 prepared by the method described in any one.
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| CN105929167A (en) * | 2016-04-29 | 2016-09-07 | 山东省农业科学院畜牧兽医研究所 | Detection test paper for beta-epinephrine receptor stimulant and preparation method of detection test paper |
| CN108627506A (en) * | 2018-03-29 | 2018-10-09 | 黑龙江八农垦大学 | The preparation method of rhodamine B colloid Buddha's warrior attendant carbon test strip in a kind of food |
| CN113189338A (en) * | 2021-04-30 | 2021-07-30 | 三峡大学 | Rhodamine B colloidal gold detection test strip and preparation method and application thereof |
| CN117147885A (en) * | 2023-11-01 | 2023-12-01 | 广东省大湾区华南理工大学聚集诱导发光高等研究院 | Reagent for jointly determining multiple drugs in hair and preparation method thereof |
| CN117147885B (en) * | 2023-11-01 | 2024-01-12 | 广东省大湾区华南理工大学聚集诱导发光高等研究院 | Reagent for jointly determining multiple drugs in hair and preparation method thereof |
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