CN206756845U - A kind of combined colloidal gold immuno-chromatography test paper strip for being used to detect two kinds of antigen - Google Patents
A kind of combined colloidal gold immuno-chromatography test paper strip for being used to detect two kinds of antigen Download PDFInfo
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- CN206756845U CN206756845U CN201720621365.5U CN201720621365U CN206756845U CN 206756845 U CN206756845 U CN 206756845U CN 201720621365 U CN201720621365 U CN 201720621365U CN 206756845 U CN206756845 U CN 206756845U
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Abstract
The utility model discloses a kind of combined colloidal gold immuno-chromatography test paper strip for being used to detect two kinds of antigen.The test strips are made up of folding left and right sides test strips, wherein：Left side test strips include bottom plate and sample pad A, the A albumen gold standard pad, nitrocellulose filter and the blotting paper that are sequentially connected from top to bottom on bottom plate, and nature controlling line A, detection line A and detection line B are provided with described nitrocellulose filter；Right side test strips include bottom plate, the blotting paper positioned at bottom plate upper end and the nitrocellulose filter being sequentially connected from top to bottom positioned at bottom plate lower end, B albumen gold standard pad and sample pad B, visual window is provided among blotting paper and nitrocellulose filter, nature controlling line B is provided with described nitrocellulose filter, the visual window is used to observe detection line A and detection line B.Using the test strips can in a test strips two kinds of foreign proteins of quick detection simultaneously, it is simple to operate, it is convenient, suitable for Fields detection.
Description
Technical field
A kind of colloidal gold immuno-chromatography test paper strip is the utility model is related to, it is more particularly to a kind of to can be used for two kinds of detection simultaneously
The combined colloidal gold immuno-chromatography test paper strip of antigen, the utility model belong to biological technical field.
Background technology
Collaurum is a kind of conventional labelling technique, is to be applied to the one of antigen-antibody using collaurum as tracer label thing
The new immunolabelling technique of kind, there is the advantages of its is unique.In recent years widely used in various biological studies.In addition, glue
The immune quick diagnosis technology of body gold is a kind of solid phase labelling novel immune detection technique, and tracer or colour developing are used as using collaurum
Agent, by colloid gold label on antibody, the reaction principle of antigen-antibody is recycled, gold grain is connected on antigen, works as antigen
When antibody response is reached to a certain degree, there is macroscopic colour developing result.Using colloid gold immune diagnostic techniques, to antibody
Bioactivity influence is small, and the factor of interference detection results is small, is with a wide range of applications.
It is CN200920244114 in Chinese Patent Application No., CN201620065094 patent application, which individually discloses, to be turned
Transgenic soybean, corn and Identification of Rice Foreign Gene detection kit and a kind of combined two-way colloidal gold immuno-chromatography test paper strip.
The structure of test strips disclosed in Application No. CN201620065094 patent application is as shown in Figure 8, it is seen that is in matter in fact
Two kinds of different test strips by device and have been connected in together, the structure of test strips significantly not improved, with this reality
It is different with novel structure principle.Application No. CN200920244114 patent application discloses a kind of genetically engineered soybean, corn
With Identification of Rice Foreign Gene detection standard molecule kit, including a closing box 1 with cover, box is built with sample loading plate 2 and one
Individual Ep pipes 3, structure are only used for lab analysis use as shown in figure 9, the detection kit complex operation, time-consuming.
The content of the invention
The shortcomings that the utility model overcomes in the prior art, there is provided one kind can be with both not synantigens of quick detection
Combined test strips, not two kinds of antigens of synantigen or two kinds of non-homologous species can be detected in allied species.
In order to achieve the above object, the following technological means that the utility model uses：
A kind of combined colloidal gold immuno-chromatography test paper strip for being used to detect two kinds of antigen, it is by the folding left and right sides
Test strips form, wherein：
Left side test strips 14 include bottom plate 17 and sample pad A1, the A egg being sequentially connected from top to bottom on bottom plate
White gold standard pad 2, nitrocellulose filter 6 and blotting paper 7, nature controlling line A 3, detection are provided with described nitrocellulose filter 6
Line A 4 and detection line B 5；Sample pad A 1, A albumen gold standard pad 2, nitrocellulose filter 6 and blotting paper 7 are on junction
Lower superposition；
Right side test strips 15 include bottom plate 18, the blotting paper 8 above bottom plate and below bottom plate on to
Under the nitrocellulose filter 10, B albumen gold standard pad 12 and the sample pad B 13 that are sequentially connected, in blotting paper 8 and cellulose nitrate
Visual window 9 is provided among plain film 10, nature controlling line B 11, the position of the visual window 9 are provided with described nitrocellulose filter 10
Put it is corresponding with detection line A 4 and detection line B 5, for observing detection line A 4 and detection line B 5；Nitrocellulose filter 10,
B albumen gold standard pad 12 and sample pad B 13 are superimposed above and below junction.
Wherein, it is preferred that described nature controlling line A3, detection line A4 and detection line B 5 is parallel to nitrocellulose filter 6
Upper following, nature controlling line A 3 is located at nature controlling line A 3 and detection line B 5 centre close to A albumen gold standard pad 2, detection line A 4.
Wherein, it is preferred that have broken line 16 among described left and right sides test strips, applied on the broken line 16 coated with waterproof
Layer, can effectively obstruct the influence while when liquid is added dropwise to opposite side test strips.
Wherein, it is preferred that described bottom plate 17 and/or bottom plate 18 are PVC bottom plates.
Wherein, it is preferred that described sample pad A 1, sample pad B 13, blotting paper 7 and blotting paper 8 is high water absorption
Paper.
Wherein, it is preferred that described blotting paper 8 is made up of two floor height blotting papers.
Described " high water adsorbing paper " is using blotting paper made of high water-absorption fiber, compared to conventional absorbent paper paper, is had more
High water absorbing properties.Wherein blotting paper 8 is made up of two blotting papers, more stronger than above-mentioned other structures water absorbing properties.
Wherein, it is preferred that answered in described A albumen gold standard pad 2 containing the collaurum-A protein monoclonal antibodies that can be redissolved
Compound, the collaurum-B protein monoclonal antibody compounds that can be redissolved are contained in described B albumen gold standard pad 12.
Wherein, it is preferred that described A albumen is different albumen or two kinds of non-homologous species in allied species from B albumen
Two kinds of albumen.
Wherein, it is preferred that described detection line A 4 is coated with A protein monoclonal antibodies, and described detection line B 5 is coated with
There are B protein monoclonal antibodies, avoid that cross reaction occurs between antigen-antibody.A protein monoclonal antibodies resist with B protein monoclonals
Body identifies different antigenic determinants respectively, and described nature controlling line A 3 is coated with A albumen antiantibodys, described nature controlling line B 11
It is coated with B albumen antiantibodys.
Wherein, it is preferred that the width of described bottom plate is more than sample pad, gold standard pad, nitrocellulose filter and blotting paper
Width.
When carrying out two kinds of antigen detections using test strips of the present utility model, carry out in accordance with the following methods：
By detected sample protein suspending liquid, it is noticeable for albumen in tissue intracellular or caryoplasm it is proposed that making
With the RIPA lysates (P0013K) in the green skies with corresponding protease inhibitor cocktail (P1015) so that suspension
Protein content is higher, increases the sensitivity of test paper.Detected sample protein suspending drop is added to the sample of left side test strips 14
Pad on A, suspension to be measured is flowed to test paper lower zone under capillary chromatography effect, when suspension can by A albumen gold standard pad 2
Collaurum-A protein monoclonal antibody compounds δ is redissolved from glass fibre membrane, collaurum-A protein monoclonal antibodies are compound
Thing δ dissociates into suspension, and it is combined with collaurum-A protein monoclonal antibody compounds δ if Staphylococal Protein A is contained in sample, shape
Into Staphylococal Protein A-A protein monoclonal antibodies-colloidal gold composite β, compound β and A gold-marking regions dissociate into suspension uncombined A
The compound δ of antigen is down migrated under osmosis, and the antiantibody of A albumen is coated with lower section nature controlling line A 3, and free answers
Compound δ is combined with A albumen antiantibodys makes colloidal gold aggregation in nature controlling line A 3 be in chromogenic reaction.Compound β in suspension continues
Down migrate when reaching detection line A 4, due to being fixed with the monoclonal antibody for Staphylococal Protein A on detection line A 4, according to dual anti-
The sandwich principle of body, compound β can be identified by insolubilized antibody combines to form colloidal gold labeled monoclonal antibody-antigen-solid phase monoclonal antibody
Compound, now colloidal gold aggregation chromogenic reaction occurs on detection line A.
After Staphylococal Protein A has been detected, a period of time is stood after left side test strips 14 are dried, B antigens can be detected, on right side
B samples are added dropwise on the sample pad B 13 of the lower zone of test strips 15, where sample under chromatography acts on upward nature controlling line B 11
Region is flowed, when suspension can be by collaurum-B protein monoclonal antibody compound α from glass fibre membrane by B albumen gold standard pad 12
Upper redissolution, collaurum-B protein monoclonal antibody compounds α dissociate into suspension, itself and glue if B antigens are contained in sample
Body gold-B protein monoclonal antibody compounds α is combined, and forms B antigens-B protein monoclonal antibodies-colloidal gold composite γ, compound
Dissociate into the suspension compound α of uncombined B antigens of thing γ and B gold-marking regions is migrated under osmosis toward top, top
B albumen antiantibodys are coated with nature controlling line B 11, free compound α is combined with B albumen antiantibodys makes colloidal gold aggregation in matter
It is in chromogenic reaction to control line B 11.After the discolorations of nature controlling line B 11, right side test strips 15 are covered into test strips to the left by broken line 16
14, the now blotting paper 8 and sample pad B 13, B albumen gold standard pad 12, the nitrocellulose filter of lower section of right side test strips 15 top
10 form countercurrent chromatography in the test strips of left side causes test substance B and compound α, γ originally in right side test strips 15
In left side, the top migration of test paper, in opposite direction with the chromatography of detection A albumen, so as to realize that testing sample can be by detection line B
5, due to being fixed with the monoclonal antibody for B antigens on detection line B 5, according to double-antibody sandwich principle, compound γ can quilt
Insolubilized antibody identification combines to form colloidal gold labeled monoclonal antibody-antigen-solid phase monoclonal antibody complex, and now colloidal gold aggregation exists
Chromogenic reaction occurs on detection line A, and then realizes detection.Need exist for stressing is fixed respectively because of detection line A, B
Be corresponding antigens monoclonal antibody, possess different antigenic determinants, respectively only have specificity to Staphylococal Protein A or B antigens, no
Cross reaction can occur.
More existing test strips are compared with kit detection, and the beneficial effects of the utility model are：
(1) test strips of the present utility model are divided into left and right sides region in structure, and each region includes two kinds respectively
The respective monoclonal antibody of albumen, pass through secondary chromatographic theory (positive reverse).Test paper is cleverly realized from disposably to can
Use repeatly, the utility model provide conveniently approach for family expenses and laboratory quick detection.
(2) it is simple to operate, quickly, two kinds of foreign proteins can be detected simultaneously in a test strips, it is not necessary to special
Instrument and equipment, easily can directly it be detected in the scene such as field.
(3) detection time is short；Result judgement standard is unified, i.e.,：When only chromogenic reaction, corresponding detection occur for nature controlling line
Explanation is negative when chromogenic reaction does not occur for line；When chromogenic reaction occurs for nature controlling line, and chromogenic reaction occurs for corresponding detection line
Explanation is positive；When chromogenic reaction does not occur for nature controlling line, then testing result is invalid.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model (top view)；
Fig. 2 is the structural representation (side view) of left side test strips；
Fig. 3 is the structural representation (side view) of right side test strips；
Fig. 4 is the schematic diagram (top view) that right side test strips are covered by broken line 13 after test strips to the left；
Fig. 5 is A Protein Detection result schematic diagrams, and which show respectively be positive, negative and invalid when testing result
When colour developing situation；
Fig. 6 is B Protein Detection result schematic diagrams, and which show respectively be positive, negative and invalid when testing result
When colour developing situation；
Fig. 7 is the testing result schematic diagram when detecting two kinds of albumen of A, B, and which show respectively when A, B Protein Detection
Result be the positive, A, B Protein Detection result be negative, A Protein Detections result be the positive/B Protein Detections result be feminine gender,
B Protein Detections result is the colour developing situation when positive/A Protein Detections result is negative and invalid；
Fig. 8 is the structural representation of the test strips disclosed in Application No. CN201620065094 patent application；
Fig. 9 is the structural representation of the kit of Application No. CN200920244114 patent application publication.
Description of reference numerals：
In Fig. 1-7：1- sample pads A；2-A albumen gold standard pads；3- nature controlling lines A；4- detection lines A；5- detection lines B；6th, 10- nitre
Acid cellulose film；7- blotting papers；8- blotting papers (two layers)；9- visual windows；11- nature controlling lines B；12-B albumen gold standard pads；13- samples
Pad B；Test strips on the left of 14-；Test strips on the right side of 15-；16- broken lines (have waterproof coating, effectively obstructed while being added dropwise at broken line
To the influence of opposite side test strips during liquid)；17th, 18-PVC bottom plates.
In Fig. 8：A- sample pads, b- gold conjugation pads, a liner plate in two groups of c-, d- nitrocellulose membranes, e- first
Detection line, the detection lines of h- second, the control lines of f- first, the control lines of i- second, g- absorbent filters.
In Fig. 9：1 '-seal box, 2 '-sample loading plate, 3 '-Ep pipes, 4 '-load sample hole.
Embodiment
The utility model is further described with reference to specific embodiment, and the advantages of the utility model and feature will be with
Description and it is apparent.But these embodiments are only exemplary, do not form any restrictions to the scope of the utility model.
It will be understood by those skilled in the art that can be to the utility model skill under without departing from spirit and scope of the present utility model
The details and form of art scheme are modified or replaced, but these modifications and replacement each fall within the scope of protection of the utility model
It is interior.
As shown in Figure 1-Figure 3, a kind of combined colloidal gold immunochromatographimethod for being used to detect two kinds of antigen of the present utility model
Test strips, it (by broken line 16, is there is waterproof coating, effectively obstructed while when liquid is added dropwise pair by foldable at broken line 16
The influence of opposite side test strips) left and right sides test strips composition, wherein：
Left side test strips 14 include bottom plate 17 and the sample pad A 1 being sequentially connected from top to bottom, A eggs on bottom plate
White gold standard pad 2, nitrocellulose filter 6 and blotting paper 7, nature controlling line A 3, detection are provided with described nitrocellulose filter 6
Line A 4 and detection line B 5；Sample pad A 1, A albumen gold standard pad 2, nitrocellulose filter 6 and blotting paper 7 are on junction
Lower superposition；
Right side test strips 15 include bottom plate 18, the blotting paper 8 above bottom plate and below bottom plate on to
Under the nitrocellulose filter 10, B albumen gold standard pad 12 and the sample pad B 13 that are sequentially connected, in blotting paper 8 and cellulose nitrate
Visual window 9 is provided among plain film 10, nature controlling line B 11, the position of the visual window 9 are provided with described nitrocellulose filter 10
Put it is corresponding with detection line A 4 and detection line B 5, for observing detection line A 4 and detection line B 5；Nitrocellulose filter 10,
B albumen gold standard pad 12 and sample pad B 13 are superimposed above and below junction.
Wherein, described nature controlling line A3, detection line A4 and detection line B 5 be parallel to the upper following of nitrocellulose filter 6,
Nature controlling line A 3 is located at nature controlling line A 3 and detection line B 5 centre close to A albumen gold standard pad 2, detection line A 4.
Wherein, there is broken line 16 among described left and right sides test strips, be coated with waterproof coating on the broken line 16, can have
The barrier of effect is while influence when liquid is added dropwise to opposite side test strips.
Wherein, described bottom plate 17 and bottom plate 18 are PVC bottom plates.
Wherein, described sample pad A1, sample pad B 13, blotting paper 7 and blotting paper 8 are high water adsorbing paper.
Wherein, described blotting paper 8 is made up of two floor height blotting papers.
Wherein, the collaurum-A protein monoclonal antibody compounds that can be redissolved, institute are contained in described A albumen gold standard pad 2
Contain the collaurum-B protein monoclonal antibody compounds that can be redissolved in the B albumen gold standard pad 12 stated.
Wherein, described A albumen is two hatching eggs of different albumen or two kinds of non-homologous species in allied species from B albumen
In vain.
Wherein, described detection line A 4 is coated with A protein monoclonal antibodies, and described detection line B 5 is coated with B albumen
Monoclonal antibody, avoid that cross reaction occurs between antigen-antibody.A protein monoclonal antibodies are distinguished with B protein monoclonal antibodies
Different antigenic determinants is identified, described nature controlling line A3 is coated with A albumen antiantibodys, and described nature controlling line B 11 is coated with B
Albumen antiantibody.
Wherein, the width of described bottom plate is more than the width of sample pad, gold standard pad, nitrocellulose filter and blotting paper.
It is illustrated below by taking the detection of transgenic crop as an example：
1st, the preparation of test strips
(1) foldable PVC bottom plates are provided, size is 12mm × 60mm, and there is broken line 16 centre, and PVC bottom plates are equally divided into
Two parts：Left side PVC bottom plates 17 and left side PVC bottom plates 18；
(2) preparation of left side test strips 14
Left side test strips 14 include PVC bottom plates 17 and the sample pad A being sequentially connected from top to bottom on bottom plate 17
1st, A albumen gold standard pad 2, nitrocellulose filter 6 and blotting paper 7, on described nitrocellulose filter 6 be provided with nature controlling line A 3,
Detection line A 4 and detection line B 5；
The coated weight of the monoclonal antibody of the anti-CP4-EPSPS albumen of collaurum-mouse is 5ng-50ng in A albumen gold standard pad 2,
The amount for being coated with sheep anti mouse antiantibody on nitrocellulose filter 6 on nature controlling line A 3 resists for 0.4 μ g-1.2 μ g, detection line A 4 coating mouse
The amount of CP4-EPSPS protein monoclonal antibodies is that 0.4 μ g-1.2 μ g, detection line B 5 the coating anti-PAT/bar protein monoclonals of mouse resist
The amount of body is 0.4 μ g-1.2 μ g.
Sample pad A 1 size is 5mm × 4mm, and the size of A albumen gold standard pad 2 is 3mm × 4mm；Nitrocellulose filter 15
Size be 4mm × 28mm；The size of blotting paper 6 is 4mm × 19mm.Sample pad A 1 and blotting paper 7 are high water adsorbing paper structure
Into
(3) preparation of right side test strips 13
Right side test strips 15 include PVC bottom plates 18, the blotting paper 8 positioned at bottom plate upper end and positioned at bottom plate lower end from upper
Nitrocellulose filter 10, B albumen gold standard pad 12 and the sample pad B 13 being sequentially connected under, it is fine in blotting paper 8 and nitric acid
Tie up and be provided with visual window 9 among plain film 10, nature controlling line B 11 is provided with described nitrocellulose filter 10, the visual window 9
Position is corresponding with detection line A 4 and detection line B 5, for observing detection line A 4 and detection line B 5.
The coated weight of the anti-PAT/bar protein monoclonal antibodies of collaurum-mouse is 5ng-50ng in B albumen gold standard pad 10, nitre
The amount for being coated with sheep anti mouse antiantibody on acid cellulose film 18 on nature controlling line B11 is 0.4 μ g-1.2 μ g.
Sample pad B 13 size is 5mm × 4mm, and the size of B albumen gold standard pad 12 is 3mm × 4mm；Nitrocellulose filter
10 size is 4mm × 10mm；The size of blotting paper 8 is 4mm × 19mm, and the size of visual window 9 is 4mm × 8mm.Sample pad B
13 and blotting paper 8 be high water adsorbing paper form, wherein blotting paper 8 is made up of two floor height blotting papers.
(4) test strips are dried 30min, produced in the case where temperature is 37 DEG C.
2nd, the joint-detection of transgenic crop soybean and rice protein
(1) preparation of protein suspending liquid
In order to improve collecting protein efficiency, we use the RIPA lysates (P0013K) in the green skies and corresponding protease
/ inhibitor mixture (P1015) increases the sensitivity of test paper so that the protein content of suspension is higher；
(2) by soybean sample protein suspending liquid to be detected, it is added drop-wise on sample pad A 1, makes under capillary chromatography effect
Suspension to be measured flows to test paper lower area, when suspension can resist collaurum-CP4-EPSPS monoclonals by A albumen gold standard pad 2
Nanocrystal composition δ redissolves from glass fibre membrane, collaurum -- and CP4-EPSPS monoclonal antibody complex δ dissociates into suspension,
If in sample contain CP4-EPSPS antigens if itself and collaurum -- CP4-EPSPS monoclonal antibody complex δ is combined, formation
CP4-EPSPS--CP4-EPSPS monoclonal antibodies-colloidal gold composite β, compound β dissociate into suspension not with A gold-marking regions
Down migrated under osmosis with reference to CP4-EPSPS compound δ, antiantibody is coated with lower section nature controlling line A 3, it is free
Compound δ is combined with sheep anti mouse antiantibody makes colloidal gold aggregation that chromogenic reaction occur in nature controlling line A3.Compound β in suspension
Continuation is down migrated when reaching detection line A 4, compound due to being fixed with CP4-EPSPS monoclonal antibody on detection line A4
Thing β can be identified by insolubilized antibody combines to form colloidal gold labeled monoclonal antibody-antigen-solid phase monoclonal antibody complex (double antibody folder
The heart), now on detection line A 4 chromogenic reaction occurs for colloidal gold aggregation.
(2) after soybean protein has been detected, a period of time is stood after left side test paper dries, can detect transgenic paddy rice
PAT/bar albumen, transgenic paddy rice PAT/bar protein suspending liquid is added dropwise on the sample pad B 13 of right side area, makees in chromatography
With lower sample, the regions of nature controlling line B 11 are flowed upward, and after the discolorations of nature controlling line B 11, right side test strips 15 are passed through into folding
Line 16 covers test strips 14 (shown in Fig. 4) to the left, due to the blotting paper 8 for having high-strength absorbing above left side so that on right side
Reverse direction chromatography is formd in test strips, so as to realize that testing sample can be by detection line B 5, and then realizes detection, by can
Form 9 observes result.
(3) result judgement
For CP4-EPSPS albumen：When chromogenic reaction occurs for only nature controlling line A, it is anti-that colour developing does not occur for corresponding detection line A
Seasonable explanation is negative；When chromogenic reaction occurs for nature controlling line A, and chromogenic reaction occurs for corresponding detection line A, explanation is positive；When
Chromogenic reaction do not occur for nature controlling line A, and corresponding detection line A chromogenic reaction occurs or when chromogenic reaction does not occur for nature controlling line A, corresponding
Detection line A chromogenic reaction does not occur then testing result is invalid, schematic diagram is as shown in Figure 5.
For PAT/bar albumen：When chromogenic reaction occurs for only nature controlling line B, chromogenic reaction does not occur for corresponding detection line B
When explanation be negative；When chromogenic reaction occurs for nature controlling line B, and chromogenic reaction occurs for corresponding detection line B, explanation is positive；Work as matter
Chromogenic reaction do not occur for control line B, and corresponding detection line B chromogenic reaction occurs or when chromogenic reaction does not occur for nature controlling line B, corresponding
Chromogenic reaction does not occur for detection line B, and then testing result is invalid, and schematic diagram is as shown in Figure 6.
When chromogenic reaction occurs for only nature controlling line A, nature controlling line B, chromogenic reaction does not occur for corresponding detection line A, detection line B
When illustrate that A, B albumen are feminine gender；When chromogenic reaction occurs for nature controlling line A, nature controlling line B, corresponding detection line A, detection line B occur
It is the positive to illustrate A, B albumen during chromogenic reaction；When chromogenic reaction occurs for nature controlling line A, nature controlling line B, corresponding detection line A occurs
Chromogenic reaction and detection line B do not occur illustrating that A albumen is positive, B albumen is negative during chromogenic reaction；When nature controlling line A, nature controlling line
Chromogenic reaction occurs for B, and chromogenic reaction occurs in corresponding detection line B and detection line A does not occur illustrating B albumen for sun during chromogenic reaction
Property, A albumen for feminine gender；When chromogenic reaction does not occur for nature controlling line A, nature controlling line B, then illustrate that testing result is invalid.Schematic diagram is such as
Shown in Fig. 7.
Experiment proves to use ELISA test strip genetically engineered soybean CP4-EPSPS of the present utility model and transgenic paddy rice albumen
PAT/bar, there is preferable anti-jamming effectiveness to complex matrices such as blade, seed and Bulk Grains, can be widely applied to two kinds of differences
The detection of antigenic substance.
Claims (10)
1. a kind of combined colloidal gold immuno-chromatography test paper strip for being used to detect two kinds of antigen, it is characterised in that by a folding left side
Right both sides test strips composition, wherein：
Left side test strips (14) include bottom plate (17) and the sample pad A (1), the A that are sequentially connected from top to bottom on bottom plate
Albumen gold standard pad (2), nitrocellulose filter (6) and blotting paper (7), Quality Control is provided with described nitrocellulose filter (6)
Line A (3), detection line A (4) and detection line B (5)；Sample pad A (1), A albumen gold standard pad (2), nitrocellulose filter (6) and
Blotting paper (7) is superimposed above and below junction；
Right side test strips (15) include bottom plate (18), the blotting paper (8) above bottom plate and below bottom plate from upper
Nitrocellulose filter (10), B albumen gold standard pad (12) and the sample pad B (13) being sequentially connected under, in blotting paper (8) and
Visual window (9) is provided among nitrocellulose filter (10), nature controlling line B (11) is provided with described nitrocellulose filter (10),
The position of the visual window (9) is corresponding with detection line A (4) and detection line B (5), for observing detection line A (4) and detection
Line B (5)；Nitrocellulose filter (10), B albumen gold standard pad (12) and sample pad B (13) are superimposed above and below junction.
2. test strips as claimed in claim 1, it is characterised in that described nature controlling line A (3), detection line A (4) and detection line
Parallel to the upper following of nitrocellulose filter (6), nature controlling line A (3) is located at B (5) close to A albumen gold standard pad (2), detection line A (4)
Nature controlling line A (3) and detection line B (5) centre.
3. test strips as claimed in claim 1, it is characterised in that have broken line (16), institute among described left and right sides test strips
State and waterproof coating is coated with broken line (16).
4. test strips as claimed in claim 1, it is characterised in that described bottom plate (17) and/or bottom plate (18) are PVC bottom plates.
5. test strips as claimed in claim 1, it is characterised in that described sample pad A (1), sample pad B (13), blotting paper
(7) and blotting paper (8) is high water adsorbing paper.
6. test strips as claimed in claim 5, it is characterised in that the blotting paper (8) is made up of two floor height blotting papers.
7. test strips as claimed in claim 1, it is characterised in that contain the glue that can be redissolved in described A albumen gold standard pad (2)
Body gold-A protein monoclonal antibody compounds, the collaurum-B albumen lists that can be redissolved are contained in described B albumen gold standard pad (12)
Clonal antibody compound.
8. test strips as claimed in claim 1, it is characterised in that described A albumen is different eggs in allied species from B albumen
White or two kinds of non-homologous species two kinds of albumen.
9. test strips as claimed in claim 1, it is characterised in that described detection line A (4) is coated with A protein monoclonals and resisted
Body, described detection line B (5) are coated with B protein monoclonal antibodies, and A protein monoclonal antibodies are distinguished with B protein monoclonal antibodies
Different antigenic determinants is identified, described nature controlling line A (3) is coated with A albumen antiantibodys, described nature controlling line B (11) coatings
There are B albumen antiantibodys.
10. test strips as claimed in claim 1, it is characterised in that the width of described bottom plate is more than sample pad, gold standard pad, nitre
The width of acid cellulose film and blotting paper.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720621365.5U CN206756845U (en) | 2017-05-31 | 2017-05-31 | A kind of combined colloidal gold immuno-chromatography test paper strip for being used to detect two kinds of antigen |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720621365.5U CN206756845U (en) | 2017-05-31 | 2017-05-31 | A kind of combined colloidal gold immuno-chromatography test paper strip for being used to detect two kinds of antigen |
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| CN206756845U true CN206756845U (en) | 2017-12-15 |
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