CN116355744A - Detection kit for synchronously detecting various porcine infectious disease viruses and application - Google Patents
Detection kit for synchronously detecting various porcine infectious disease viruses and application Download PDFInfo
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- CN116355744A CN116355744A CN202310609689.7A CN202310609689A CN116355744A CN 116355744 A CN116355744 A CN 116355744A CN 202310609689 A CN202310609689 A CN 202310609689A CN 116355744 A CN116355744 A CN 116355744A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to the field of infectious virus detection, and particularly discloses a detection kit for synchronously detecting various porcine infectious viruses and application thereof.
Description
Technical Field
The invention relates to the field of infectious virus detection, and particularly discloses a detection kit for synchronously detecting various porcine infectious viruses and application thereof.
Background
The virus is a non-cell life form, it is formed from a nucleic acid long chain and protein shell, the virus has no self-metabolism mechanism and no enzyme system, so that the virus can leave host cell, and can be formed into chemical substance which has no life activity and can not be independently self-propagated, once it is entered into host cell, it can utilize the material and energy in cell and the ability of replication, transcription and translation, and can produce new-generation virus identical to that of it according to the genetic information contained in its own nucleic acid, and the kit is a box for holding chemical reagents for detecting chemical component, medicine residue and virus kind, etc., usually the detection kit contains test tube, chemical reagent for extracting nucleic acid and inspection reagent card for detecting virus.
The detection reagents in the detection reagent cards for detecting different viruses are different, if the detection reagents are mixed together, the efficacy can be lost, meanwhile, the traditional detection reagent cards can only drip nucleic acid into one detection reagent card when dripping the accounting extracting solution, so that multiple viruses cannot be detected at the same time, multiple repeated operations are needed, multiple viruses are detected, the time is wasted, and meanwhile, the phenomenon of reagent pollution is more easily caused by multiple operations, so that the detection result is inaccurate.
In order to solve the above-mentioned problems, it is necessary to provide a detection kit for synchronously detecting various porcine infectious disease viruses and an application thereof.
Disclosure of Invention
The invention provides a detection kit capable of detecting various porcine infectious disease viruses simultaneously, so that the occurrence of reagent pollution caused by the fact that the kit is wasted due to repeated operation for many times is prevented, and application of the detection kit.
The technical scheme provided by the invention is as follows: the utility model provides a detect reagent box for synchronous detection of multiple pig infectious disease virus, includes reagent pick-up plate, the reagent pick-up plate has arranged a plurality of side by side, set up the detection groove on the reagent pick-up plate, the fixed mounting has different detection membranes that contain different antigen diagnostic reagent in the detection groove in a plurality of reagent pick-up plates, is connected by the connecting plate between every two reagent pick-up plates that side by side, the connecting plate is arranged the both ends about the reagent pick-up plate, just the both ends of connecting plate are articulated with two adjacent reagent pick-up plates respectively, are provided with the solid mechanism that keeps two reagent pick-up plates parallel expansion between two adjacent reagent pick-up plates, are provided with the through-flow mechanism that is connected with the detection membrane between all reagent pick-up plates.
Preferably, the two reagent detecting plates are fixedly provided with synchronous gears at the hinging positions of the two reagent detecting plates and the synchronous gears on the same side of the two adjacent reagent detecting plates are meshed with each other.
Preferably, the fixing mechanism comprises a fixing rod, fixing clamping rods and a baffle plate, the fixing rod is rotatably erected on one of the reagent detection plates, the fixing clamping rods which are connected with plastics are fixedly connected to the left side and the right side of the reagent detection plate respectively, protrusions are machined at the opposite ends of the fixing clamping rods, two identical fixing clamping rods are correspondingly arranged on the other adjacent reagent detection plate, and the baffle plate is fixedly connected to one end, away from the fixing clamping rods, of the other reagent detection plate.
Preferably, the flow-through mechanism comprises flow-through grooves, inclined grooves and flow-through pipes, wherein the flow-through grooves are formed in the same position along the front-back direction in a penetrating manner, the inclined grooves which are inclined and are used for connecting the flow-through grooves with the detection films are formed in the reagent detection plates, the upper ends of the reagent detection plates positioned at the forefront ends are provided with inflow inlets which are communicated with the flow-through grooves corresponding to the flow-through grooves, and the flow-through pipes are connected between the flow-through grooves on the two adjacent reagent detection plates.
Preferably, the left side and the right side of the inner wall of the draft tube are provided with notches.
Preferably, a guide rod is erected in the inclined groove, and the guide rod looks like a through groove.
Preferably, the two adjacent reagent detection plates are provided with a tube closing mechanism at the position corresponding to the through flow tube, the tube closing mechanism comprises a tube closing slide plate, a jacking spring and a tube closing roller, the reagent detection plates are provided with tube closing grooves at the position corresponding to the through flow tube, the tube closing slide plate is connected in a sliding manner in the tube closing grooves, the jacking spring is fixedly connected between the tube closing slide plate and the bottom surface of the tube closing grooves, and the exposed ends of the tube closing slide plates on the two adjacent reagent detection plates are connected with the tube closing roller in a common rotation manner.
Preferably, a supporting mechanism is arranged at the forefront end of the forefront reagent detection plate; the supporting mechanism comprises a supporting rod and a blocking rod, the left side and the right side of the forefront end of the reagent detection plate are respectively hinged with one supporting rod, the reagent detects and is being close to the below fixed connection blocking lever at its left and right sides both ends at the prop up the pole, prop up the pole and be made for plastics, and prop up the pole and be two segmentation forms, and be great sliding connection of friction between the two sections.
Preferably, an annular breaking groove is formed in one end, close to the reagent detection plate, of the supporting rod.
The invention also provides application of the detection kit for synchronously detecting the porcine infectious disease viruses, which is applied to synchronous detection of the porcine infectious disease viruses and comprises the following steps of;
s1: all the reagent detection plates are in an unfolded state through the fixing mechanism;
s2: injecting the nucleic acid extraction solution into the detection membrane on each reagent detection plate by a through-flow mechanism;
s3: and judging whether the porcine infectious virus is positive or not by detecting the display condition on the membrane.
The beneficial effects of the invention are as follows:
(1) According to the invention, all reagent detection plates are in an unfolded state through the orientation fixing mechanism, and the nucleic acid extracting solution is injected into the detection film on each reagent detection plate through the circulation mechanism, so that a plurality of infectious viruses are detected simultaneously.
(2) According to the invention, the notches are formed in the left side and the right side of the inner wall of the draft tube, so that the bending of the draft tube and the mutual adhesion of the inner wall of the draft tube are facilitated, and the detection reagent in each reagent detection plate is in an isolated state, so that the mutual pollution is prevented.
(3) According to the invention, the reagent detection plate is in an inclined state by rotating the supporting rod and supporting the supporting rod by using the blocking rod, so that the nucleic acid extracting solution can flow in the through flow groove and the through flow pipe.
Drawings
The invention will be further described with reference to the drawings and examples.
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is an enlarged schematic view of the structure A in FIG. 1 according to the present invention;
FIG. 3 is an enlarged schematic view of the structure of FIG. 1B according to the present invention;
FIG. 4 is an enlarged schematic view of the structure of FIG. 1 at C in accordance with the present invention;
FIG. 5 is a schematic view of a flow mechanism according to the present invention;
FIG. 6 is a schematic view of the installation position of the tube closing mechanism of the present invention;
FIG. 7 is an enlarged schematic view of the structure of FIG. 6D according to the present invention;
FIG. 8 is a schematic view of the structure of the supporting rod of the present invention;
FIG. 9 is an enlarged schematic view of the structure of FIG. 8 at E in accordance with the present invention;
fig. 10 is a schematic cross-sectional view of a draught tube according to the present invention.
In the figure:
1. a reagent detection plate; 11. a detection groove; 12. a detection membrane; 13. a connecting plate; 14. an inflow port; 15. a closed tube groove; 131. a synchronizing gear;
2. a direction fixing mechanism; 21. a directional fixing rod; 22. a fixing clamp rod; 23. a baffle;
3. a through-flow mechanism; 31. a flow through groove; 32. an inclined groove; 33. a draft tube; 34. a guide rod;
4. a tube closing mechanism; 41. a closed tube slide plate; 42. a pressing spring; 43. a tube closing roller;
5. a prop-up mechanism; 51. a prop up rod; 511. breaking the groove; 52. blocking lever.
Detailed Description
The invention is further described in connection with the following detailed description in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
Embodiment one:
as shown in fig. 1, 2 and 5, the invention provides a detection kit for synchronously detecting various porcine infectious disease viruses, which comprises a plurality of reagent detection plates 1, wherein the reagent detection plates 1 are arranged side by side, detection grooves 11 are formed in the reagent detection plates 1, different detection films 12 containing different antigen diagnosis reagents are fixedly arranged in the detection grooves 11 in the plurality of reagent detection plates 1, each two parallel reagent detection plates 1 are connected by a connecting plate 13, the connecting plates 13 are arranged at the left end and the right end of each reagent detection plate 1, two ends of each connecting plate 13 are respectively hinged with two adjacent reagent detection plates 1, a fixing mechanism 2 for keeping the two reagent detection plates 1 spread in parallel is arranged between the two adjacent reagent detection plates 1, a through-flow mechanism 3 connected with the detection films 12 is arranged between all the reagent detection plates 1, as shown in fig. 1, 2 and 3, a synchronous gear 131 is fixedly arranged at the hinge joint of each two reagent detection plates 1 and the two adjacent reagent detection plates 1 are meshed with each other by the synchronous gear 131 on the same side of each reagent detection plate 1.
The components of the detection reagents in the detection films 12 of each reagent detection plate 1 are different, when the reagent detection plates 1 are used, all the reagent detection plates 1 are in an unfolded state through the fixing mechanism 2, the nucleic acid extracting solution is injected into the detection films 12 on each reagent detection plate 1 through the through-flow mechanism 3, and whether the porcine infectious virus is positive or not is judged through the display condition on the detection films 12, so that a plurality of infectious viruses are detected simultaneously, and in the unfolding process, the adjacent reagent detection plates 1 can be in a synchronous unfolded state through the action of the synchronous gear 131, so that all the reagent detection plates 1 are prevented from being in a bent state.
As shown in fig. 1 and 2, the fixing mechanism 2 includes a fixing rod 21, a fixing clamping rod 22 and a baffle 23, the fixing rod 21 is rotatably mounted on one of the reagent detecting boards 1, the left and right sides of the reagent detecting board 1 are respectively and fixedly connected with a fixing clamping rod 22 with plastic connection, the opposite ends of the fixing clamping rod 22 are processed with protrusions, two identical fixing clamping rods 22 are correspondingly arranged on the adjacent other reagent detecting board 1, and the baffle 23 is fixedly connected with one end, away from the fixing clamping rod 21, of the fixing clamping rod 22 of the other reagent detecting board 1.
When the reagent detecting plate 1 is secured in the unfolded state by the fixing mechanism 2, the fixing rod 21 is taken out from the two fixing connecting rods of the reagent detecting plate 1 rotatably connected with the fixing rod 21, is clamped between the two fixing clamping rods 22 of the other adjacent reagent detecting plate 1, and is blocked by the baffle plate 23, so that the adjacent two reagent detecting plates 1 are in the unfolded state.
As shown in fig. 5 and 10, the flow-through mechanism 3 includes a flow-through groove 31, an inclined groove 32 and a flow-through pipe 33, each reagent detecting plate 1 is provided with the flow-through groove 31 penetrating back and forth at the same position, the reagent detecting plate 1 is provided with the inclined groove 32 connecting the flow-through groove 31 with the detecting film 12, the upper end of the reagent detecting plate 1 located at the forefront end is provided with the inflow port 14 corresponding to the flow-through groove 31 and communicated with the flow-through groove 31, and the flow-through pipes 33 are connected between the flow-through grooves 31 on two adjacent reagent detecting plates 1.
It should be noted that, by adding the nucleic acid extracting solution into the inflow port 14, the nucleic acid extracting solution flows through the through flow groove 31 to the inclined groove 32, and finally contacts with the detection membrane 12, and flows through the through flow pipe 33 to the through flow groove 31 in the next detection kit, and by providing notches on the left and right sides of the inner wall of the through flow pipe 33, when all the reagent detection plates 1 are in the undeployed state, the bending of the through flow pipe 33 and the mutual adhesion of the inner walls of the through flow pipe 33 are facilitated, so that the detection reagents in each reagent detection plate 1 are in the isolated state, and the mutual pollution is prevented, the guide rod 34 is erected in the inclined groove 32, and the guide rod 34 looks like the through flow groove 31, so that the nucleic acid extracting solution is facilitated to flow into the inclined groove 32.
Embodiment two:
as shown in fig. 6 and 7, the two adjacent reagent detecting boards 1 are provided with a tube closing mechanism 4 at the position corresponding to the through-flow tube 33, the tube closing mechanism 4 comprises a tube closing slide plate 41, a top pressure spring 42 and a tube closing roller 43, the reagent detecting boards 1 are provided with a tube closing groove 15 at the position corresponding to the through-flow tube 33, the tube closing slide plate 41 is connected in the tube closing groove 15 in a sliding manner, the top pressure spring 42 is fixedly connected between the tube closing slide plate 41 and the bottom surface of the tube closing groove 15, and the exposed ends of the tube closing slide plates 41 on the two adjacent reagent detecting boards 1 are connected with the tube closing roller 43 in a rotating manner.
When all the reagent detecting plates 1 are in the undeployed state, the pressure is applied to the closed tube slide 41 by the pressing spring 42, so that the closed tube roller 43 is pressed against the draft tube 33, thereby further increasing the tightness of the draft tube 33.
As shown in fig. 1, 4 and 8, the foremost end of the foremost reagent detection plate 1 is provided with a lifting mechanism 5; the supporting mechanism 5 comprises a supporting rod 51 and a blocking rod 52, wherein the left side and the right side of the foremost end of the reagent detection plate 1 are respectively hinged with the supporting rod 51, the reagent detection is fixedly connected with the blocking rod below the supporting rod 51 which is closer to the left end and the right end of the supporting rod, the supporting rod 51 is made of plastics, the supporting rod 51 is in a two-section shape, and the two sections are in sliding connection with larger friction.
It should be noted that, when the nucleic acid extracting solution is dripped, the supporting rod 51 is rotated and the blocking rod 52 is used to prop against the supporting rod 51, so that the reagent detecting plate 1 is in an inclined state, thereby being beneficial to the flow of the nucleic acid extracting solution in the through flow groove 31 and the through flow pipe 33, as shown in fig. 9, the annular breaking groove 511 is formed at one end of the supporting rod 51, which is close to the reagent detecting plate 1, so that when the dripping of the nucleic acid extracting solution is completed, the supporting rod 51 can be broken through the breaking groove 511, thereby being convenient for the level of the reagent detecting plate 1 and the subsequent detecting operation.
The invention also provides an application of the detection kit for synchronously detecting various porcine infectious disease viruses, which comprises the following steps:
s1: all the reagent detection boards 1 are in a unfolded state through the fixing mechanism 2, and in the unfolding process, the adjacent reagent detection boards 1 can be in a synchronous unfolding state through the action of the synchronous gears 131, so that all the reagent detection boards 1 are prevented from being in a bent state;
s2: injecting a nucleic acid extracting solution into the detection membrane 12 on each reagent detection plate 1 through the flow-through mechanism 3, wherein in the process, the nucleic acid extracting solution flows to the inclined groove 32 through the flow-through groove 31 by adding the nucleic acid extracting solution into the inflow port 14, finally contacts with the detection membrane 12, and flows to the flow-through groove 31 in the next detection kit through the flow-through pipe 33;
s3: the presence or absence of a swine infectious virus is judged by detecting the presence of a positive swine infectious virus on the membrane 12.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the foregoing examples, and that the foregoing description and description are merely illustrative of the principles of this invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A detection kit for synchronously detecting a plurality of porcine infectious disease viruses, which comprises a reagent detection plate (1);
the reagent detection board (1) has arranged a plurality of side by side, offer detection groove (11), its characterized in that on reagent detection board (1):
different detection films (12) containing different antigen diagnostic reagents are fixedly arranged in the detection grooves (11) in the plurality of reagent detection plates (1), each two parallel reagent detection plates (1) are connected by a connecting plate (13), the connecting plates (13) are arranged at the left end and the right end of each reagent detection plate (1), the two ends of each connecting plate (13) are respectively hinged with two adjacent reagent detection plates (1), a fixing mechanism (2) for keeping the two reagent detection plates (1) to be unfolded in parallel is arranged between the two adjacent reagent detection plates (1), a through-flow mechanism (3) connected with the detection films (12) is arranged between all the reagent detection plates (1),
the fixing mechanism (2) comprises a fixing rod (21), a fixing clamping rod (22) and a baffle plate (23);
the fixing rod (21) is rotatably arranged on one of the reagent detection plates (1), the left side and the right side of the reagent detection plate (1) are respectively and fixedly connected with a fixing clamping rod (22) which is connected with plastics, the opposite ends of the fixing clamping rods (22) are provided with bulges, the other adjacent reagent detection plate (1) is correspondingly provided with the same two fixing clamping rods (22), and one end, far away from the fixing clamping rods (21), of the fixing clamping rod (22) of the other reagent detection plate (1) is fixedly connected with a baffle plate (23);
the flow-through mechanism (3) comprises a flow-through groove (31), an inclined groove (32) and a flow-through pipe (33),
each reagent detection plate (1) is provided with a through flow groove (31) in a penetrating mode along the front and back at the same position, the reagent detection plate (1) is provided with an inclined groove (32) which is inclined and connects the through flow groove (31) with the detection film (12), the upper end of the reagent detection plate (1) positioned at the forefront end is provided with an inflow port (14) which is communicated with the through flow groove (31) corresponding to the through flow groove (31), and the through flow grooves (31) of two adjacent reagent detection plates (1) are connected by a through flow pipe (33).
2. A test kit for simultaneous detection of multiple swine infectious disease viruses according to claim 1, wherein two reagent test plates (1) are fixedly mounted with a synchronizing gear (131) at a hinge joint with the connecting plate (13), and the synchronizing gears (131) on the same side of two adjacent reagent test plates (1) are engaged with each other.
3. The detection kit for synchronously detecting the porcine infectious disease viruses according to claim 2, wherein the left side and the right side of the inner wall of the draft tube (33) are provided with notches.
4. A test kit for simultaneous detection of a plurality of swine infectious disease viruses according to claim 1 or 3, wherein a deflector rod (34) is mounted in the inclined groove (32), and the deflector rod (34) extends into the flow-through groove (31).
5. The detection kit for synchronously detecting the porcine infectious disease viruses according to claim 1, wherein the two adjacent reagent detection plates (1) are provided with a closed tube mechanism (4) at positions corresponding to the through tubes (33), the closed tube mechanism (4) comprises a closed tube sliding plate (41), a jacking spring (42) and a closed tube roller (43), the reagent detection plates (1) are provided with closed tube grooves (15) at positions corresponding to the through tubes (33), the closed tube sliding plate (41) is connected in a sliding manner in the closed tube grooves (15), the jacking spring (42) is fixedly connected between the closed tube sliding plate (41) and the bottom surfaces of the closed tube grooves (15), and the exposed ends of the closed tube sliding plates (41) on the two adjacent reagent detection plates (1) are connected with the closed tube roller (43) in a common rotation manner.
6. The detection kit for synchronously detecting the porcine infectious disease viruses according to claim 1, wherein the foremost reagent detection plate (1) is provided with a supporting mechanism (5), the supporting mechanism (5) comprises supporting rods (51) and blocking rods (52), the left side and the right side of the foremost end of the reagent detection plate (1) are respectively hinged with one supporting rod (51), the reagent detection is fixedly connected with blocking bars below the lower parts of the supporting rods (51) which are closer to the left end and the right end of the supporting rods, the supporting rods (51) are made of plastics, the supporting rods (51) are two sections, and sliding connection with larger friction is arranged between the two sections.
7. The detection kit for synchronously detecting a plurality of porcine infectious disease viruses according to claim 6, wherein one end of the supporting rod (51) close to the reagent detection plate (1) is provided with an annular breaking groove (511).
8. Use of a detection kit for simultaneous detection of a plurality of porcine infectious disease viruses according to any of claims 1-7, for simultaneous detection of a plurality of porcine infectious disease viruses, comprising the steps of;
s1: all the reagent detection plates (1) are in an unfolded state through the fixing mechanism (2);
s2: injecting the nucleic acid extraction solution into the detection membrane (12) on each reagent detection plate (1) through the flow-through mechanism (3);
s3: and judging whether the swine infectious virus is positive or not by detecting the display condition on the membrane (12).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310609689.7A CN116355744B (en) | 2023-05-29 | 2023-05-29 | Detection kit for synchronously detecting various porcine infectious disease viruses and application |
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| CN202310609689.7A CN116355744B (en) | 2023-05-29 | 2023-05-29 | Detection kit for synchronously detecting various porcine infectious disease viruses and application |
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| CN116355744B CN116355744B (en) | 2023-09-12 |
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