CN209260070U - A kind of Novel free instrument nucleic acid on-site detection card - Google Patents
A kind of Novel free instrument nucleic acid on-site detection card Download PDFInfo
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- CN209260070U CN209260070U CN201920841315.7U CN201920841315U CN209260070U CN 209260070 U CN209260070 U CN 209260070U CN 201920841315 U CN201920841315 U CN 201920841315U CN 209260070 U CN209260070 U CN 209260070U
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- nucleic acid
- filter membrane
- elastic container
- fluid passage
- acid extraction
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Abstract
The utility model belongs to nucleic acid detection technique field, it detects and blocks more particularly to a kind of Novel free instrument nucleic acid on-site, including card body, sample pretreatment device, the card body is equipped with nucleic acid extraction unit, nucleic acid amplification detection unit, it is characterized in that, the nucleic acid extraction unit is equipped with injection port, nucleic acid extraction module, the nucleic acid extraction module is successively sealedly connected with reagent storage extrusion system from top to bottom, one-way pressure valve, filter membrane switching system, waste liquid tank, the injection port and the nucleic acid extraction module are tightly connected, the nucleic acid extraction unit and the nucleic acid amplification detection unit are tightly connected.Whole processes such as nucleic acid extraction, amplification, detection are integrated into a detection card by the utility model detection card, are supported, and only need layman's simple operations without instrument, that is, are realized the field quick detection from sample to result.
Description
Technical field
The utility model belongs to nucleic acid detection technique field, and in particular to a kind of Novel free instrument nucleic acid on-site detection card.
Background technique
Nucleic acid detection technique largely needs to rely on instrument at present, and to sample treatment, detection environment and peopleware
It is required that all very high.Primary conventional detection of nucleic acids, from sample is obtained to nucleic acid purification, detection architecture configuration is reacted to result and sentences
It reads, generally requires a few hours to the longer time.These factors all limit nucleic acid detection technique quickly detection immediately at the scene
Realization.
There is a small amount of research to report the exploration of the nucleic acid on-site detection method for exempting from instrument at present, but these methods are true
Just it is applied to on-site test, there is problems: (1) based on the methods of test paper amplification-microballoon/colloidal gold/film hybridization colour developing
Nucleic acid detection method be although able to achieve the nucleic acid amplification for exempting from instrument and detection of nucleic acids the results show that but generally requiring to extract in advance
Purified sample of nucleic acid, can not be directly in complicated and diversified all types of sample nucleic acid detections on site.(2) based on hand-held
Formula filter membrane method or paramagnetic particle method extract nucleic acid, then dock subsequent cracking, reaction, detection and etc. (reacted with paper base, freeze-dried reagent
It is more for redissolving reaction) technology may be implemented to exempt from extraction-amplification-testing process of instrument, but it is cumbersome, being easy step more
Error, while pollution risk is big, it is also difficult to it is widely applied.
Utility model content
The utility model aim is solution the above-mentioned problems in the prior art, provides a kind of Novel free instrument core
Sour on-site test card.Whole processes such as nucleic acid extraction, amplification, detection are integrated into a detection card by the detection card, are not necessarily to
Any instrument is relied on, and only needs layman's simple operations, that is, realizes the field quick detection from sample to result.
In order to solve the above technical problems, the utility model adopts the following technical solution:
A kind of Novel free instrument nucleic acid on-site detection card, including card body, sample pretreatment device, the card body are equipped with nucleic acid
Extraction unit, nucleic acid amplification detection unit, the nucleic acid extraction unit are equipped with injection port, nucleic acid extraction module, and the nucleic acid mentions
Modulus block is successively sealedly connected with reagent storage extrusion system, one-way pressure valve, filter membrane switching system, waste liquid tank from top to bottom,
The injection port and the nucleic acid extraction module are tightly connected, and the nucleic acid extraction unit and the nucleic acid amplification detection unit are close
Envelope connection.
The filter membrane switching system is equipped with fluid passage, sliding rail and the filter membrane being mounted on sliding rail and pushes
Device, the part that the filter membrane pusher is located inside filter membrane switching system are loaded with filter membrane.
The sample pretreatment device is liquid container, and prepackage is used for the pretreated reaction solution of nucleic acid extraction.
The reagent storage extrusion system is equipped with elastic container, the liquid extruder track above the elastic container
And with its mating liquid extruder being mounted on the liquid extruder track.
Further, the liquid extruder is to the elastic container direction protrusion, positioned at the side of the elastic container.
Further, the elastic container quantity is n, and wherein n is the integer of > 0, and the fluid passage quantity is institute
The n+1 of elastic container quantity is stated, the filter membrane is parallel to the cross section of the fluid passage and can pass through the fluid passage.
Further, when n=2, the reagent storage extrusion system is successively arranged the first elastic container, second from left to right
Elastic container, first elastic container is interior to be full of cleaning solution or other nucleic acid extraction related liquids, second elastic container
Full of nucleic acid eluents, the filter membrane switching system be equipped with 3 the first not connected fluid passages, second liquid channel,
Third fluid passage, the first fluid passage first half are communicated with the injection port, latter half and the waste liquid tank phase
It is logical；Second liquid channel first half is communicated with the one-way pressure valve on first elastic container, latter half
It is communicated with the waste liquid tank；The one-way pressure valve on the third fluid passage first half and second elastic container
It communicates, latter half is communicated with the nucleic acid amplification detection unit.
Further, the sliding rail be equipped with the one-to-one position location of the fluid passage, guarantee filter membrane most
The liquid of the fluid passage is come into contact in a large area, reaction sufficiently, reduces liquid leakage loss.Liquid container further, institute
Stating reaction solution is lysate.
Further, the liquid container is test tube, centrifuge tube, bottle, any one in PCR pipe.
Further, the liquid extruder track perpendicular to the elastic container and is firmly connected with it.
Further, the reagent storage extrusion system is equipped with elastic container, is preinstalled with liquid relevant to nucleic acid extraction.
Further, the nucleic acid amplification unit is equipped with reaction chamber.
Further, the nucleic acid amplification unit is equipped with temperature control device, and the reaction chamber is contacted with temperature control device.Further
Ground, the number of chambers are n, and n is the integer greater than 0.
Preferably, the number of chambers is 1, and reaction chamber is preinstalled with amplification reaction system and detection reaction system.
Preferably, the number of chambers is 2, and the first reaction chamber is preinstalled with the amplification reaction system, the second reaction
Room is preinstalled with the detection reaction system, is connect between second reaction chamber and the first reaction chamber by valve or transmission device
Touching.
Preferably, amplification reaction system is the reaction system of isothermal duplication.The isothermal duplication is ring mediated isothermal amplification
Technology (LAMP), rolling circle amplification (RCA), relies on unwindase DNA isothermal at the amplification (NASBA) dependent on nucleic acid sequence
One of amplification (HDA), chain substitution amplification (SDA), recombination polymeric enzymatic amplification (RPA).
Further, the amplification reaction system is liquid, powder, appointing in freeze-drying reaction system on other media
It anticipates one kind.
Further, the other media are the scraps of paper, all-glass paper, plastic sheet, any one in film, preferably glass
Glass fibrous paper.
Further, second reaction chamber is visualization transparent sealing chamber, and second reaction chamber is equipped with detection body
System.
Further, the detection architecture is the principles such as colloidal gold method, fluorescence method, metachromasia, hybrid method, microballoon method
Any one in detection architecture, preferably colloidal gold method.
Further, the temperature control device is exothermic material or electric heater unit.
Further, the exothermic material is any one in iron powder, quick lime and a variety of exothermic material mixtures.
The utility model uses above-mentioned technical proposal, has the advantages that
1, original sample is added from injection port or after the sample of sample pre-treatment device processing, passes through switching filter membrane switching
System filter membrane position and mobile liquid extruder, drive liquid in elastic container to pass sequentially through filter membrane, complete nucleic acid extraction process；
Extract obtained nucleic acid and be directly entered nucleic acid amplification detection unit and carry out augmentation detection, without using any instrument can be completed from
It extracts amplification and arrive a whole set of foranalysis of nucleic acids process detected again, scene can be obtained testing result, easy to operate, inexpensive,
It is portable, it avoids pollution simultaneously.
2, applied widely, it can be used for a variety of disease detections or genetic test based on foranalysis of nucleic acids, flexibly configurable mentions
System response liquid port number is taken, and different amplification of arranging in pairs or groups, testing principle complete extraction, the amplification, testing process of nucleic acid.
Detailed description of the invention
Fig. 1 is the card body structural schematic diagram that the utility model exempts from that instrument nucleic acid on-site detects card preferred embodiment；
Fig. 2 shows for the filter membrane switching system internal structure that the utility model exempts from the detection card preferred embodiment of instrument nucleic acid on-site
It is intended to.
In figure: 1- injection port, 2- filter membrane switching system, 3- waste liquid tank, 4- filter membrane pusher, the first elastic container of 5-, 6-
Liquid extruder, 7- one-way pressure valve, 8- liquid extruder track, the second elastic container of 9-, 10- fluidic connection channels, 11-
One reaction chamber, 12- valve, the second reaction chamber of 13-, 14-temperature control devices, 21- the first fluid passage first half, the first liquid of 22-
Body channel latter half, 23- second liquid channel latter half, 24- third fluid passage latter half, 25- sliding rail,
The first position location 41-, the second position location 42-, 43- third position location.
Specific embodiment
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, it can also be obtained according to these attached drawings other attached drawings.
Embodiment 1.
As shown in Figs. 1-2, a kind of Novel free instrument nucleic acid on-site detection card, including card body, sample pretreatment device, sample
Pretreating device is test tube, is preinstalled with reaction solution, and the cracking for sample is handled.
Card body is equipped with nucleic acid extraction unit, nucleic acid amplification detection unit.
Nucleic acid extraction unit is equipped with injection port (1), nucleic acid extraction module, and successively sealing connects nucleic acid extraction module from top to bottom
It is connected to reagent storage extrusion system, one-way pressure valve (7), filter membrane switching system (2), waste liquid tank (3), injection port (1) and nucleic acid
Extraction module is tightly connected, and nucleic acid extraction unit and nucleic acid amplification detection unit are tightly connected by fluidic connection channels (10).
Filter membrane switching system (2) is equipped with 3 not connected fluid passages, sliding rail (25) and is mounted on sliding
Filter membrane pusher (4) on track (25), filter membrane pusher (4) are located at the internal part of filter membrane switching system (2) and are loaded with filter membrane.
Reagent storage extrusion system is set there are two elastic container, two liquid extruder tracks above elastic container
(8) and with its mating liquid extruder (6) being mounted on liquid extruder track (8), liquid extruder track (8) is vertical
In elastic container and it is firmly connected with it.
Reagent storage extrusion system is successively arranged the first elastic container (5), the second elastic container (9) from left to right, and first
Cleaning solution or other nucleic acid extraction related liquids are full of in elastic container (5), the second elastic container (9) is full of eluent.Liquid
Extruder (6) is located at the left side of the first elastic container (5) to elastic container protrusion.
3 not connected fluid passages are additionally provided in filter membrane switching system (2), filter membrane is parallel to the fluid passage
Cross section, the first fluid passage first half (21) are communicated with injection port (1), the first fluid passage latter half (22) and waste liquid
Slot (3) communicates；Second logical fluid passage first half is communicated with the one-way pressure valve (7) on the first elastic container (5), the second liquid
Body channel latter half (23) is communicated with waste liquid tank (3)；The third fluid passage first half and second elastic container
(9) one-way pressure valve (7) on communicates, and third fluid passage latter half (24) is communicated with the nucleic acid amplification detection unit,
Push filter membrane pusher (4) filter membrane can be realized successively to switch along track in each fluid passage, to the nucleic acid on filter membrane into
Row cleaning, elution.Sliding rail (25) is equipped with and one-to-one 3 position locations of fluid passage, guarantee filter membrane largest face
Product touches reaction solution, and reaction is abundant.
Nucleic acid amplification unit is equipped with the first reaction chamber (11) for nucleic acid amplification and the second reaction for detection of nucleic acids
Room (13) and for reaction the temperature control device (14) of preference temperature is provided.First reaction chamber (11) is preinstalled with amplification reaction system,
Preferably, it is preinstalled with the reaction system of recombination polymeric enzymatic amplification (RPA).Second reaction chamber (13) is preinstalled with based on colloidal gold principle
The test strips for detecting nucleic acid are contacted between the second reaction chamber (13) and the first reaction chamber (11) by valve (12), realize nucleic acid
Transfer.
The nucleic acid extraction unit of the utility model is based on filter membrane method nucleic acid extraction Technology design, filter membrane method nucleic acid rapidly extracting
Technology extracts the nucleic acid of various samples, the molecular diagnosis for nucleic acid using the pellosil of energy specific adsorption nucleic acid.
Filter membrane method nucleic acid rapidly extracting technology mainly utilizes pellosil in the case where with high salt and low pH value, can specific adsorption core
Acid；And the characteristics of nucleic acid can be discharged when less salt and high pH value obtain, by change flow through pellosil liquid PH and from
Sub- concentration, quickly and efficiently to carry out nucleic acid extraction.
The use step of the present embodiment are as follows: 1, cracking: sample and lysate are mixed well in sample pre-treatment device；
2, it adsorbs: the mixed liquor in sample pretreatment device being transferred to detection card by injection port (1), filter membrane initial position is first
Position location (41), mixed liquor flows through the first fluid passage first half (21) and is contacted with filter membrane, on filter membrane pusher (4)
Filter membrane the nucleic acid in mixed liquor is adsorbed, waste liquid is transferred to waste liquid tank by the first fluid passage latter half (22)；
3, it cleans: shifting filter membrane pusher (4) onto the second position location (42), liquid extruder (6) is pushed from left to right, by first
Cleaning solution in elastic container (5) is squeezed out through one-way pressure valve (7), and to clean the nucleic acid being adsorbed on filter membrane, waste liquid is by the
Two fluid passage latter halfs (23) are transferred to waste liquid tank (3)；4, it elutes: shifting filter membrane pusher (4) onto third position location
(43), continue to the right to push liquid extruder (6), to squeeze out the eluent in the second elastic container (9), on filter membrane
Nucleic Acid Elution gets off to flow through third fluid passage latter half (24), fluidic connection channels (10), and it is anti-that nucleic acid is transferred to first
Answer room (11).5, expand: the nucleic acid eluted is reacted with reaction solution, realizes the amplification of nucleic acid；6, it detects: after the completion of amplification,
Valve (12) are opened, nucleic acid is transferred to the second reaction chamber (13) and carries out detection reading result.
Embodiment 2.
A kind of Novel free instrument nucleic acid on-site detection card, including card body, sample pretreatment device, sample pretreatment device be
Test tube is preinstalled with reaction solution, and the cracking for sample is handled.
Card body is equipped with nucleic acid extraction unit, nucleic acid amplification detection unit.
Nucleic acid extraction unit is equipped with injection port (1), nucleic acid extraction module, and successively sealing connects nucleic acid extraction module from top to bottom
It is connected to reagent storage extrusion system, one-way pressure valve (7), filter membrane switching system (2), waste liquid tank (3), injection port (1) and nucleic acid
Extraction module is tightly connected, and nucleic acid extraction unit and nucleic acid amplification detection unit are tightly connected by fluidic connection channels (10).
Filter membrane switching system (2) is equipped with 2 fluid passages, sliding rail (25) and is mounted on sliding rail (25)
Filter membrane pusher (4), filter membrane pusher (4) is located at the internal part of filter membrane switching system (2) and is loaded with filter membrane.
Reagent stores extrusion system and squeezes equipped with the elastic container equipped with eluent, 1 liquid above elastic container
Out device track (8) and with its mating liquid extruder (6) being mounted on liquid extruder track (8).Liquid extruder (6)
To elastic container protrusion, positioned at the side of elastic container.
Filter membrane switching system (2) is equipped with 2 not connected fluid passages, and filter membrane is parallel to the cross section of fluid passage
And fluid passage can be passed through, the first fluid passage first half (21) is communicated with injection port (1), latter half and waste liquid tank (3)
It communicates；Second liquid channel first half is communicated with the one-way pressure valve (7) on elastic container, and latter half and nucleic acid amplification are examined
Unit (13) are surveyed to communicate.Sliding rail (25) is equipped with and the one-to-one position location of fluid passage, guarantee filter membrane largest face
Product touches reaction solution, and reaction is abundant.
Nucleic acid amplification unit is equipped with the reaction chamber for nucleic acid amplification detection, is preinstalled with the reaction system and inspection of isothermal duplication
Reaction system is surveyed, realizes the amplification and detection of nucleic acid.
Above description is only a specific implementation of the present invention, but the protection scope of the utility model is not limited to
In this, any change or replacement expected without creative work be should be covered within the scope of the utility model.
Therefore, the protection scope of the utility model should be determined by the scope of protection defined in the claims.
Claims (8)
1. a kind of Novel free instrument nucleic acid on-site detection card, including card body, sample pretreatment device, the card body are mentioned equipped with nucleic acid
Take unit, nucleic acid amplification detection unit, which is characterized in that the nucleic acid extraction unit is equipped with injection port, nucleic acid extraction module, institute
It states nucleic acid extraction module and is successively sealedly connected with reagent storage extrusion system, one-way pressure valve, filter membrane switching system, waste liquid tank,
The injection port and the nucleic acid extraction module are tightly connected, and the nucleic acid extraction unit and the nucleic acid amplification detection unit are close
Envelope connection.
2. Novel free instrument nucleic acid on-site detection card as described in claim 1, which is characterized in that in the filter membrane switching system
Equipped with fluid passage, sliding rail and the filter membrane pusher being mounted on sliding rail, the filter membrane pusher is located at filter membrane
Part inside switching system is loaded with filter membrane.
3. Novel free instrument nucleic acid on-site detection card as described in claim 1, which is characterized in that the sample pretreatment device
For liquid container, prepackage is used for the pretreated reaction solution of nucleic acid extraction.
4. Novel free instrument nucleic acid on-site detection card as claimed in claim 2, which is characterized in that the reagent storage squeezes out system
System is equipped with elastic container, the liquid extruder track above the elastic container and mating is mounted on the liquid with its
Liquid extruder on extruder track.
5. Novel free instrument nucleic acid on-site as claimed in claim 4 detection card, which is characterized in that the liquid extruder is to institute
Elastic container direction protrusion is stated, positioned at the side of the elastic container.
6. Novel free instrument nucleic acid on-site as claimed in claim 4 detection card, which is characterized in that the elastic container quantity is
N, wherein n is the integer of > 0, and the fluid passage quantity is the n+1 of the elastic container quantity, and the filter membrane is parallel to institute
It states the cross section of fluid passage and the fluid passage can be passed through.
7. Novel free instrument nucleic acid on-site detection card as claimed in claim 6, which is characterized in that when n=2, the reagent storage
Extrusion system is successively arranged the first elastic container, the second elastic container from left to right, is full of cleaning in first elastic container
Liquid or nucleic acid extraction related liquid, second elastic container are full of nucleic acid eluents, and the filter membrane switching system is equipped with 3
Not connected the first fluid passage, second liquid channel, third fluid passage, the first fluid passage first half and institute
It states injection port to communicate, latter half is communicated with the waste liquid tank；Second liquid channel first half and first elasticity
The one-way pressure valve on container communicates, and latter half is communicated with the waste liquid tank；The third fluid passage first half
It is communicated with the one-way pressure valve on second elastic container, latter half is communicated with the nucleic acid amplification detection unit.
8. the Novel free instrument nucleic acid on-site as described in 2,4,5,6,7 any one of claim detects card, which is characterized in that institute
Sliding rail is stated to be equipped with and the one-to-one position location of the fluid passage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920841315.7U CN209260070U (en) | 2019-06-05 | 2019-06-05 | A kind of Novel free instrument nucleic acid on-site detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920841315.7U CN209260070U (en) | 2019-06-05 | 2019-06-05 | A kind of Novel free instrument nucleic acid on-site detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209260070U true CN209260070U (en) | 2019-08-16 |
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ID=67585706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920841315.7U Active CN209260070U (en) | 2019-06-05 | 2019-06-05 | A kind of Novel free instrument nucleic acid on-site detection card |
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| CN (1) | CN209260070U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113881560A (en) * | 2021-11-15 | 2022-01-04 | 深圳市研元生物科技有限公司 | Nucleic acid detecting plate embedded in gene chip |
-
2019
- 2019-06-05 CN CN201920841315.7U patent/CN209260070U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113881560A (en) * | 2021-11-15 | 2022-01-04 | 深圳市研元生物科技有限公司 | Nucleic acid detecting plate embedded in gene chip |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191009 Address after: 510000 Unit 406, Building A01, 78 Luntou Road, Haizhu District, Guangzhou City, Guangdong Province Co-patentee after: Guangzhou universal Junan Biotechnology Co., Ltd. Patentee after: Guangzhou Universal Lihua Technology Co., Ltd. Address before: 510000 Unit 406, Building A01, 78 Luntou Road, Haizhu District, Guangzhou City, Guangdong Province Patentee before: Guangzhou Universal Lihua Technology Co., Ltd. |